diff --git a/docs/.vuepress/components/IterativeCycle.vue b/docs/.vuepress/components/IterativeCycle.vue index db7e9af8c6fdc40742ad2884b695a2639a2ec9b9..8867f89eb49e4db2cdea596241c2f81ecd61daa0 100644 --- a/docs/.vuepress/components/IterativeCycle.vue +++ b/docs/.vuepress/components/IterativeCycle.vue @@ -184,13 +184,13 @@ export default { <p>The indophenol blue test is used to detect the presence of ammonia to check the functionality of the nitrite reductase. It exploits the electrophilicity of the ammonium ion to link to phenol molecules, thus creating a conjugated system that is able to absorb in the red region of the visible light, yielding a deep-blue dye. The reaction starts by treating a sample suspected to contain ammonium with methanol, keeping the ammonia in its cationic form, and sodium hypochlorite to convert the ammonium in chloramine gas, which will stay dissolved in the methanol solution. The chloramine in methanol solution is then added to a mixture containing phenol to yield a chloramine to phenol molar ratio of 1:2 and sodium nitroprusside, which will serve as a catalyst. The reaction mixture then turns blue to signal that it has come to an end (Indophenol reaction).</p> <!-- Image with figure caption --> <figure style="text-align: center; margin: 20px 0;"> - <img src="https://static.igem.wiki/teams/5306/engineering/indophenol-reaction-scheme.webp" alt="Indophenol Test" style="width:100%; height:auto;" /> + <img src="https://static.igem.wiki/teams/5306/engineering/indophenol-formation-park-et-al-2009.png" alt="Indophenol Test" style="width:100%; height:auto;" /> <figcaption style="font-style: italic;">Figure 19. Indophenol blue reaction scheme. Image sourced from Sasongko, A. (2018).</figcaption> </figure> <p>The concentration of both nitrites or ammonia can then be measured by measuring the absorbance of the solution and comparing to a previously made calibration curve to know exactly the amount of substrate measured.</p> <!-- Image with figure caption --> <figure style="text-align: center; margin: 20px 0;"> - <img src="https://static.igem.wiki/teams/5306/engineering/beer-lambert-s-law.webp" alt="Beer Lambert Law" style="width:100%; height:auto;" /> + <img src="https://static.igem.wiki/teams/5306/engineering/beer-lambert-law.jpg" alt="Beer Lambert Law" style="width:100%; height:auto;" /> <figcaption style="font-style: italic;">Figure 20. Beer-Lambert law. Image sourced from Bhuyan, S., (2024)..</figcaption> </figure> `