From 217de5857f651bf3ba3534007b3f1c7f504907dd Mon Sep 17 00:00:00 2001
From: Aadityanshu Abhinav <aaadityanshu@gmail.com>
Date: Wed, 2 Oct 2024 12:56:01 +0000
Subject: [PATCH] Update file notebook.html
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wiki/pages/notebook.html | 2 +-
1 file changed, 1 insertion(+), 1 deletion(-)
diff --git a/wiki/pages/notebook.html b/wiki/pages/notebook.html
index 1d5e1c8..27bb46b 100644
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<h3 style="text-align:justify"><b>Bacteria</b></h3>
<ul style="text-align:justify">
<li>We found a protocol which used a defined differential media - NBRISSM. We decided to swap the magnesium trisilicate suggested by the media with calcium aluminosilicate to suit the requirements of our studies.</li>
- <li>n order to make NBRISSM media as per the protocol, we prepared hydroxyapatite in our lab after consulting with experts from the Materials Science Lab at IIT Madras.</li>
+ <li>In order to make NBRISSM media as per the protocol, we prepared hydroxyapatite in our lab after consulting with experts from the Materials Science Lab at IIT Madras.</li>
<li>We inoculated <em>P. fluorescens</em> in our modified NBRISSM media with different BCP (indicator) concentrations and placed it in a shaking incubator at 30°C and observed every 24 hours for any color change.</li>
<li>We established an experimental protocol for inoculating <em>P. fluorescens</em> in the Martian soil simulant after consulting Prof. K Chandraraj, a soil microbiology expert at IIT Madras.
We created a cell suspension of <em>P. fluorescens</em> in fresh LB and poured in martian soil simulant. The flask with inoculated soil was kept in a shaking incubator at 30°C. 1 gm of this soil was used the next day to count CFU. We noticed contamination while observing CFU.</li>
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