diff --git a/wiki/pages/results.html b/wiki/pages/results.html index 43fbaea330e74be4cbb61207d40a6609bb28d9ec..9650ddb61daf05907accc10147c22d8de4936d2c 100644 --- a/wiki/pages/results.html +++ b/wiki/pages/results.html @@ -23,10 +23,10 @@ <div class="row" style="text-align: center; padding: 10px;"> <div class="col-4 content" style="margin: 0 auto; cursor: pointer;" onclick="showResultsResilin('pmk')"> - <h2 style="padding: 10px;">PMK...</h2> + <h2 style="padding: 10px;">pMK1</h2> </div> <div class="col-4 content" style="margin: 0 auto; cursor: pointer;" onclick="showResultsResilin('oligo')"> - <h2 style="padding: 10px;">Oligo-Methode</h2> + <h2 style="padding: 10px;">Repeatigo Method</h2> </div> </div> @@ -34,17 +34,33 @@ <div class="row" id="pmk"> <div class="col-lg-6"> <p> - <b>pMK1 With Four Resilin Repeats</b><br> - For the specific properties of our hydrogel, we needed to synthesize resilin consisting of 32–64 repeats to meet our requirements. A simple way to achieve this was by using the pMK1 vector, which was kindly provided by Elke Deuerling. By designing repeats flanked by two type IIS endonucleases, it is theoretically possible to generate as many repeats as needed for the intended application. <br> - The design of our repeats (RE4) flanked by BsmBI and BsaI recognition sites was successfully completed. These restriction sites were additionally flanked by SacI and NdeI to facilitate ligation into the pMK1 vector. The pMK1 vector and the RE4 fragment, which was provided by IDT, were both digested with the appropriate restriction enzymes. <br> - Subsequently, RE4 was successfully cloned into the pMK1 vector, resulting in the plasmid pMK1_RE4. The results were confirmed through restriction digestion (Fig. 1) and sequencing. - + <b>pMK1 with four Resilin repeats</b><br> + For the specific properties of our hydrogel, we needed to synthesize resilin consisting of 32–64 repeats to meet our requirements. A simple way to achieve this was by using the pMK1 vector, which was kindly provided by Elke Deuerling. By designing repeats flanked by two type IIS endonucleases, it is theoretically possible to generate as many repeats as needed for the intended application. + <br> + The design of our repeats (RE4) flanked by BsmBI and BsaI recognition sites was successfully completed. These restriction sites were additionally flanked by SacI and NdeI to facilitate ligation into the pMK1 vector. The pMK1 vector and the RE4 fragment, which was provided by IDT, were both digested with the appropriate restriction enzymes. + <br> + Subsequently, RE4 was successfully cloned into the pMK1 vector, resulting in the plasmid pMK1_RE4. The results were confirmed through restriction digestion (see Fig. 1) and sequencing. + </p> </div> <div class="col-lg-6"> <div style="text-align: center;"> - <img src="https://static.igem.wiki/teams/5077/results-1.svg" alt="" style="width: 50%;"> - <p>Fig. 1: 1 % agarose gel after double digest of pMK1 and pMK1_R4 with SacI and BsaI. Lane 2 and 5 with digested pMK1 shows two bands at around 3000 bp and 600 bp. Lane 3-5 and 6-9 with digested pMK1_R4 show two bands each at around 3000 bp and 200 bp. + <img src="https://static.igem.wiki/teams/5077/result/figure-1.svg" alt="" style="width: 50%;"> + <p> + Fig. 1: 1 % agarose gel after double digest of pMK1 and pMK1_R4 with SacI and BsaI. Lane 2 and 5 with digested pMK1 shows two bands at around 3000 bp and 600 bp. Lane 3-5 and 6-9 with digested pMK1_R4 show two bands each at around 3000 bp and 200 bp. + </p> + </div> + </div> + <div class="col-lg-6"> + <p> + Additionally the insertion was confirmed by Sanger sequencing (see Fig. 2). The sequencing also showed that there where no mutionas in the RE4 sequence. </p> + </div> + <div class="col-lg-6"> + <div style="text-align: center;"> + <img src="https://static.igem.wiki/teams/5077/result/figure-2.webp" alt="" style="width: 50%;"> + <p> + Fig. 2: Sequencing result of the RE4 region in the pMK1_RE4 Plasmid. On A is shown the first halve of the RE4 insertion and on B is shown the second half of the RE4 insertion. + </p> </div> </div> </div>