diff --git a/static/css/menu.css b/static/css/menu.css index 01972f3cd28c9f64dd028f205b1e43f63fe457a1..f3fbd5273a4347e1538bb181b838d5d2e2a916d6 100644 --- a/static/css/menu.css +++ b/static/css/menu.css @@ -183,6 +183,7 @@ -ms-transform: translateY(-100%); -o-transform: translateY(-100%); overflow: auto; + padding: 2%; } .menu__responsive.active { @@ -227,6 +228,7 @@ background-color: transparent; border: none; z-index: 1; + padding: 2%; } .btn-close-menu-resp i { @@ -259,7 +261,7 @@ .menu__options-resposive ul li { list-style: none; text-align: center; - width: 30%; + width: 50%; } .menu__options-resposive ul li a { diff --git a/static/css/proyect/contribution.css b/static/css/proyect/contribution.css index 3c7d1454f6012f151df32588940de4563615b38b..66d13c817ec82a3fafb04347aabbe9c2528cf2bb 100644 --- a/static/css/proyect/contribution.css +++ b/static/css/proyect/contribution.css @@ -25,3 +25,35 @@ } } + +.content__parts > p a{ + font-weight: var(--font-bold); + color: var(--color-black); +} + +.table-parts-overview { + width: 100%; + margin-bottom: 20px; + text-align: center; + overflow: auto; +} + +.table-parts-overview table { + width: 60%; + margin: auto; + border-collapse: collapse; +} + +.table-parts-overview table th, +.table-parts-overview table td { + width: 25%; + min-width: 200px; + border: 1px solid var(--border-color); + padding: 10px; +} + +.table-parts-overview table th { + background-color: var(--color-primary); + color: var(--color-white); + padding: 10px; +} diff --git a/static/css/proyect/engineering.css b/static/css/proyect/engineering.css index 7c3ad18723c7c7b5a8a93f877938a4c92f390f00..35eee053d19af1a562a7ae9845dd7a9ce48de4bf 100644 --- a/static/css/proyect/engineering.css +++ b/static/css/proyect/engineering.css @@ -374,7 +374,7 @@ .img_engineering{ width: 100%; - max-width: 700px !important; + max-width: 500px !important; } .text__title-normal-ol { diff --git a/static/css/proyect/results.css b/static/css/proyect/results.css index ed7a62151c25d0f26cd23fce76bc9383f22be3c7..bb38b6b61fa5af4f5eb214b54be02c7832ea558b 100644 --- a/static/css/proyect/results.css +++ b/static/css/proyect/results.css @@ -32,6 +32,11 @@ width: 100%; } + +.content__results>p a{ + color: var(--color-black); +} + .text__center-results { text-align: center !important; } diff --git a/static/js/menu.js b/static/js/menu.js index 1e264ffab078afca813dd4c4d2d6b7b60087c32f..657af3ab71dc4c648c8f84190e33f9b1ec7dea94 100644 --- a/static/js/menu.js +++ b/static/js/menu.js @@ -36,6 +36,7 @@ window.addEventListener('resize', activeDesactive); button_navbar.addEventListener('click', function () { if (button_navbar.classList.contains('active')) { menu_responsive.classList.remove('inactive'); + deleteOptionsActive(); menu_responsive.classList.add('active'); } else { menu_responsive.classList.remove('active'); @@ -64,9 +65,18 @@ view_options.forEach((option_view, index) => { } else { sub_menu__options_resposive[index].classList.remove("inactive"); + deleteOptionsActive(); sub_menu__options_resposive[index].classList.add("active"); option_view.children[0].innerHTML = `${nameOptions[index]} <i class="fa-solid fa-chevron-up"></i>`; } }); }); + + +function deleteOptionsActive() { + sub_menu__options_resposive.forEach((option, index) => { + option.classList.remove("active"); + view_options[index].children[0].innerHTML = `${nameOptions[index]} <i class="fa-solid fa-chevron-down"></i>`; + }); +} \ No newline at end of file diff --git a/wiki/pages/contribution.html b/wiki/pages/contribution.html index cc66885515db4ee76f1f1e49b5d624b809ee370c..5928e4b17e9fde5a57856e1cdc1241c5bd99fea4 100644 --- a/wiki/pages/contribution.html +++ b/wiki/pages/contribution.html @@ -18,8 +18,13 @@ endblock %} <!-- section Introduction --> <h1 data-aos="fade-up" data-aos-anchor-placement="center-bottom" data-aos-duration="1000">Introduction</h1> - <p data-aos="fade-up" data-aos-anchor-placement="center-bottom" data-aos-duration="1000">In line with this - educational approach, we also developed a board game based on protocols commonly used by iGEM + <p data-aos="fade-up" data-aos-anchor-placement="center-bottom" data-aos-duration="1000"> + We have added new parts to the registry with information of its sequences and some details from the literature. + The information for each “parts registry†is in <a + href="https://www.google.com/url?q=https://2024.igem.wiki/bolivia/parts&sa=D&source=docs&ust=1727814970412387&usg=AOvVaw3w3Sc66zFhwnzvAwqVjVAu">parts</a> + These parts include inorganic and organic mercury sensors, + reporters and composite parts that improve the selectivity of organic mercury detection. <br><br> + In line with this educational approach, we also developed a board game based on protocols commonly used by iGEM teams for creating a bacterial biosensor. The game is designed to help newcomers to synthetic biology easily understand the steps of this procedure. It can also be used at science fairs and outreach events to teach the general public about the technical processes behind each project in an accessible and engaging way. <br> <br> @@ -29,13 +34,66 @@ endblock %} Mercury Game. We believe this interactive game will help others learn through play and can be valuable for many future iGEM teams, especially as a tool for engaging children in scientific outreach. </p> + <h1>Parts overview</h1> + <div class="table-parts-overview"> + <table> + <thead> + <tr> + <th>New iGEM parts</th> + <th>Type</th> + <th>Part's name</th> + <th>Description</th> + </tr> + </thead> + <tbody> + <tr> + <td><a href="https://parts.igem.org/Part:BBa_K5257010">BBa_K5257010</a></td> + <td>Basic, Sensor</td> + <td>merR<sub>m4-1</sub></td> + <td>aTF repressor-activator sensitive to inorganic and organic mercury</td> + </tr> + <tr> + <td><a href="https://parts.igem.org/Part:BBa_K5257011">BBa_K5257011</a></td> + <td>Basic, Sensor</td> + <td>merR<sub>tn501</sub></td> + <td>aTF repressor-activator sensitive to inorganic mercury</td> + </tr> + <tr> + <td><a href="https://parts.igem.org/Part:BBa_K5257004">BBa_K5257004</a></td> + <td>Basic, Reporter</td> + <td>sfGFP<sub>mChartreuse</sub></td> + <td>Optimized green fluorescent reporter</td> + </tr> + <tr> + <td><a href="https://parts.igem.org/Part:BBa_K5257000">BBa_K5257000</a></td> + <td>Basic, Reporter</td> + <td>mRFP<sub>_Magenta</sub></td> + <td>Magenta fluorescent chromoprotein</td> + </tr> + <tr> + <td><a href="https://parts.igem.org/Part:BBa_K5257025">BBa_K5257025</a></td> + <td>Composite</td> + <td>NOT(TetR,TEV)-mRFP1magenta</td> + <td>Magenta reporter controlled by NOT logic gate.</td> + </tr> + <tr> + <td><a href="https://parts.igem.org/Part:BBa_K5257023">BBa_K5257023</a></td> + <td>Composite</td> + <td>LOT-MerA-GDH</td> + <td>Enzymes on the cell surface, a system for volatilization of Hg<sup>2+</sup> without the need for NADPH. + </td> + </tr> + </tbody> + </table> + </div> <!-- section Mercury Game --> <h1 data-aos="fade-up" data-aos-anchor-placement="center-bottom" data-aos-duration="1000">Mercury Game</h1> <p data-aos="fade-up" data-aos-anchor-placement="center-bottom" data-aos-duration="1000"> Welcome to the Mercury Game!<br> Mercury, widely used in mining, is also a silent threat to the environment and human health. Through this game, - you’ll learn about the dangers of mercury, its impact on ecosystems, and how it accumulates in the food chain.<br><br> + you’ll learn about the dangers of mercury, its impact on ecosystems, and how it accumulates in the food + chain.<br><br> How much do you know about this element? As you progress, you’ll discover important facts about mercury and its influence on our surroundings. The goal is to leave you with a greater understanding of its effects and the need for solutions like our mercury biosensor.<br> <br> diff --git a/wiki/pages/design.html b/wiki/pages/design.html index 5a85bba984fbdf73d301818ebbabbcb8fac48b17..8e81eca558f5c71f3432d1c25182b62ff42320d0 100644 --- a/wiki/pages/design.html +++ b/wiki/pages/design.html @@ -5,8 +5,7 @@ {% block page_content %} <div class="content__title-img" id="inicio"> <h1 class="title__parts">Design</h1> - <img class="zoom" src="https://static.igem.wiki/teams/5257/project/desing/banner-desing-result-2.webp" - alt="Design"> + <img class="zoom" src="https://static.igem.wiki/teams/5257/project/desing/banner-desing-result-2.webp" alt="Design"> </div> <div class="container__desing content_page complete_page my-5 "> <h1>Introduction</h1> @@ -58,11 +57,11 @@ </p> <ul> <li><span class="li__title-bold">Construct 1:</span> Equipped with merRm4-1, which is sensitive to both organic - and inorganic mercury - (Lian et al., 2014). When methylmercury is detected, it activates mRFP_Magenta, producing magenta + and inorganic mercury (Zhu et al. 2023). When methylmercury is detected, it activates mRFP_Magenta, producing magenta fluorescence. TetR blocks this signal in the presence of inorganic mercury (Hg²âº). </li> - <li><span class="li__title-bold">Construct 2:</span> Contains merRtn501, which detects inorganic mercury (Hg²âº) + <li><span class="li__title-bold">Construct 2:</span> Contains merRtn501 (Lian et al., 2014), which detects + inorganic mercury (Hg²âº) and triggers the expression of mChartreuse, producing green fluorescence. TetR is also expressed, binding to the tetO site to prevent magenta fluorescence when Hg²⺠is detected. @@ -139,8 +138,7 @@ Hg²⺠ions are attracted and reduced to Hgâ°. While elemental mercury can enter the cell, it does not activate the reporter gene. The transporter used to localize proteins to the membrane is Lpp-OmpT, which anchors the DogCatcher proteins. DogTags, which bind to DogCatchers, transport proteins to the outer - membrane. Gene fusions Lpp-OmpT-DogCatcher and MerA-DogTag were included on the same plasmid but under - different promoters.<br> + membrane. Gene fusions Lpp-OmpT-DogCatcher and MerA-DogTag were included on the same plasmid.<br> Mercury reductase (MerA), an intracellular enzyme requiring cofactors, is anchored to the outer membrane. To support this, we incorporated a cofactor regeneration system. MerA requires NADPH, which is replenished by glucose dehydrogenase (GDH). GDH uses glucose as its substrate, ensuring a constant @@ -279,6 +277,12 @@ Negative Feedback Circuit. Frontiers in Bioengineering and Biotechnology, 9. <a href="https://doi.org/10.3389/fbioe.2021.799781">https://doi.org/10.3389/fbioe.2021.799781</a> </li> + <li> + Zhu, K., Chen, D., Cai, Y., Zhang, T., Ma, J., Bao, L., & Chen, S. (2023). Engineering the Ultrasensitive + Visual Whole-Cell Biosensors by Evolved MerR and 5′ UTR for Detection of Ultratrace Mercury. Environmental + Science & Technology, 57(44), 16964-16973. + <a href="https://doi.org/10.1021/acs.est.3c04915">https://doi.org/10.1021/acs.est.3c04915</a> + </li> </ul> </div> diff --git a/wiki/pages/engineering.html b/wiki/pages/engineering.html index 95abfd41ef007355421e6b5cfe0015749d79527e..69d37435ceac0980bc11138b755a1615701ccf42 100644 --- a/wiki/pages/engineering.html +++ b/wiki/pages/engineering.html @@ -10,8 +10,8 @@ endblock %} <div class="container_page"> <div class="content__title-img" id="inicio"> <h1 class="title__parts title__parts-biosafety">Engineering Success</h1> - <img class="zoom" src="https://static.igem.wiki/teams/5257/project/engineering/banner-result.webp" - alt="Engineering Success"> + <img class="zoom" src="https://static.igem.wiki/teams/5257/project/engineering/banner-result.webp" + alt="Engineering Success"> </div> <div class="sidebar__content active"> <button class="btn-sidebar"><i class="fa-solid fa-xmark"></i></button> @@ -28,6 +28,8 @@ endblock %} <div class="content_engineering content_page incomplete_page"> <h1 id="overview">Overview</h1> <p class="text__overview"> + <img class="img_engineering" + src="https://static.igem.wiki/teams/5257/project/engineering/overview-result-2-1.webp" alt="overview"> Our mercury biosensor aims to provide an innovative tool for the rapid and precise detection of mercury in water, with a particular focus on organic mercury. By leveraging genetically engineered bacteria, we sought to create a biosensor capable of identifying toxic concentrations of mercury in environmental settings, @@ -37,8 +39,7 @@ endblock %} development process. This iterative approach was instrumental in refining each stage of the biosensor’s evolution. </p> - <img class="img_engineering" - src="https://static.igem.wiki/teams/5257/project/engineering/overview-result-2-1.webp" alt="overview"> + <h1 id="desing">Design</h1> <p>The iGEM Bolivia team developed two innovative strategies for creating a biosensor capable of selectively detecting both organic and inorganic mercury. These designs incorporate genes and promoters optimized for @@ -110,8 +111,10 @@ endblock %} fluorescence as an indicator of organic mercury. <br> <br> In the Presence of Inorganic Mercury (Hg²âº): Inorganic mercury binds to the merRtn501 transcription factor, which triggers the expression of the mChartreuse reporter, emitting green fluorescence. While - inorganic mercury can also bind to MerRm4-1, the expression of the magenta reporter is suppressed by - TetR, a repressor protein encoded by the tetR gene. TetR binds to the tetO site, effectively preventing + inorganic mercury can also bind to MerRm4-1, the reporter is degraded due to a proteolysis mechanism + controlled by the TEV enzyme, this enzyme exposes a degradation tag. At the same time the expression of + the magenta reporter is suppressed by tetR, a repressor protein encoded by the tetR gene. TetR binds to + the tetO site, effectively preventing mRFP_Magenta expression and ensuring that no magenta signal is emitted in response to inorganic mercury. </p> </div> @@ -121,9 +124,9 @@ endblock %} blocked by TetR-mediated repression. </p> <h2>Strategy 2: Selective Mercury Detection via Volatilization of Inorganic Mercury.</h2> - <p>This strategy utilizes a "NOT logic gate" mechanism to differentiate between organic and inorganic mercury, - ensuring that the biosensor is selectively responsive to organic mercury, while inhibiting responses to - inorganic mercury. + <p> + This strategy volatilizes inorganic mercury out of the cell before it enters the biosensor, ensuring that the + biosensor responds selectively to organic mercury. </p> @@ -158,7 +161,7 @@ endblock %} </div> </div> </div> - + <div class="content_strategy-imgs"> <div class="section__img strategy2"> <img src="https://static.igem.wiki/teams/5257/project/engineering/strategy2/4-input-con-hg-2-result.webp" @@ -317,8 +320,10 @@ endblock %} measure the total mercury concentration present in these samples using atomic absorption spectroscopy, ensuring a comprehensive understanding of mercury levels in natural water bodies. </P> - <img src="https://static.igem.wiki/teams/5257/project/engineering/detection-methylmercury-1-result-1.webp" alt=""> - <img src="https://static.igem.wiki/teams/5257/project/engineering/detection-methylmercury-2-result-1.webp" alt=""> + <img src="https://static.igem.wiki/teams/5257/project/engineering/detection-methylmercury-1-result-1.webp" + alt=""> + <img src="https://static.igem.wiki/teams/5257/project/engineering/detection-methylmercury-2-result-1.webp" + alt=""> <h4>Detection of heavy metals and measurement of gfp protein fluorescence.</h4> <h2>Biosensor characterization</h2> @@ -388,7 +393,7 @@ endblock %} MerA-expressing <span class=" coli-cursive"> </div> </div> - + <h2>Phase 2: Complete System Characterization</h2> <p>The full enzyme system will be assessed for its ability to reduce inorganic mercury in the presence and absence of NADPH. After culturing, aliquots will be collected and mercury concentrations in the supernatant diff --git a/wiki/pages/hardware.html b/wiki/pages/hardware.html index 541f6e9925589c7b83b88c763f6990eb67c5a766..a020b9efd00756b788b08107a84df7162225cd71 100644 --- a/wiki/pages/hardware.html +++ b/wiki/pages/hardware.html @@ -289,71 +289,84 @@ accessible to our community.{% endblock %} <table> <thead> <tr> - <th>Price</th> - <th>Quantity</th> <th>Component</th> + <th>Quantity</th> + <th>Price</th> + <th>Total</th> </tr> </thead> <tbody> <tr> - <td>11$</td> - <td>1</td> <td>ESP32 MICROCONTROLLER</td> + <td>1</td> + <td>11$</td> + <td>11$</td> </tr> <tr> - <td>0.5$</td> - <td>1</td> <td>LED UV MT5355-UV</td> + <td>1</td> + <td>0.5$</td> + <td>0.5$</td> </tr> <tr> - <td>10$</td> - <td>1</td> <td>OLED screen 128 X 64</td> + <td>1</td> + <td>10$</td> + <td>10$</td> </tr> <tr> - <td>2$</td> - <td>1</td> <td>ADC Module ADS1117</td> + <td>1</td> + <td>2$</td> + <td>2$</td> </tr> <tr> - <td>1$</td> - <td>1</td> <td>LDR SENSOR</td> + <td>1</td> + <td>1$</td> + <td>1$</td> </tr> <tr> - <td>0.5$</td> - <td>1</td> <td>ON/OFF BUTTON</td> + <td>1</td> + <td>0.5$</td> + <td>0.5$</td> </tr> <tr> - <td>1$</td> - <td>1</td> <td>PUSH BUTTONS</td> + <td>1</td> + <td>1$</td> + <td>1$</td> </tr> <tr> - <td>13$</td> - <td>1</td> <td>battery pack 2600 mAh</td> + <td>1</td> + <td>13$</td> + <td>13$</td> </tr> <tr> - <td>3.5$</td> - <td>1</td> <td>battery charger TP4856</td> + <td>1</td> + <td>3.5$</td> + <td>3.5$</td> </tr> <tr> - <td>12.5$</td> - <td>2</td> <td>3D PRINTED PARTS</td> + <td>2</td> + <td>12.5$</td> + <td>25$</td> </tr> <tr> - <td>10$</td> - <td>1</td> <td>UV BLOCK FILTER</td> + <td>1</td> + <td>10$</td> + <td>10$</td> </tr> <tr> - <td>4$</td> - <td>1</td> <td>CABLES AND ACCESSORIES FOR ASSEMBLY</td> + <td>1</td> + <td>4$</td> + <td>4$</td> </tr> </tbody> </table> @@ -364,16 +377,26 @@ accessible to our community.{% endblock %} <div class="links__appendices"> <span>Programming code</span><br> <ul> - <li><a href="https://static.igem.wiki/teams/5257/project/hardware/codigo-programaci-n/mercurito-code.pdf">Marcurito code</a></li> + <li><a + href="https://static.igem.wiki/teams/5257/project/hardware/codigo-programaci-n/mercurito-code.pdf">Marcurito + code</a></li> </ul> <span>3D models</span><br> <ul> <li><a href="https://static.igem.wiki/teams/5257/project/hardware/3d-models-stl/box.pdf">Box </a> <li><a href="https://static.igem.wiki/teams/5257/project/hardware/3d-models-stl/ensamblaje-1.pdf">Assembly </a> - <li><a href="https://static.igem.wiki/teams/5257/project/hardware/3d-models-stl/fluorometro-experimental.pdf">Experimental fluorometer </a> - <li><a href="https://static.igem.wiki/teams/5257/project/hardware/3d-models-stl/parte-plana-de-tapa-deslizante1.pdf">Flat part of sliding lid </a> - <li><a href="https://static.igem.wiki/teams/5257/project/hardware/3d-models-stl/parte-plana-de-tapa-deslizante1.pdf">Sliding lid </a> - <li><a href="https://static.igem.wiki/teams/5257/project/hardware/3d-models-stl/parte-plana-de-tapa-deslizante1.pdf">Slide lid </a> + <li><a + href="https://static.igem.wiki/teams/5257/project/hardware/3d-models-stl/fluorometro-experimental.pdf">Experimental + fluorometer </a> + <li><a + href="https://static.igem.wiki/teams/5257/project/hardware/3d-models-stl/parte-plana-de-tapa-deslizante1.pdf">Flat + part of sliding lid </a> + <li><a + href="https://static.igem.wiki/teams/5257/project/hardware/3d-models-stl/parte-plana-de-tapa-deslizante1.pdf">Sliding + lid </a> + <li><a + href="https://static.igem.wiki/teams/5257/project/hardware/3d-models-stl/parte-plana-de-tapa-deslizante1.pdf">Slide + lid </a> </ul> diff --git a/wiki/pages/parts.html b/wiki/pages/parts.html index e27349975147de06a2120a8d44333647d0a3d5da..5e3de2732ee2f6086cc7935ce610fb1ca72565a0 100644 --- a/wiki/pages/parts.html +++ b/wiki/pages/parts.html @@ -25,7 +25,7 @@ <th>Function</th> <th>Construct</th> <th>Code</th> - <th>Category</th> + <th>iGEM parts</th> </tr> <tr> <td>Pc<span>429-1</span></td> @@ -59,7 +59,7 @@ <th>Function</th> <th>Construct</th> <th>Code</th> - <th>Category</th> + <th>iGEM parts</th> </tr> <tr> <td>P<span>Tn21</span></td> @@ -93,7 +93,7 @@ <th>Function</th> <th>Construct</th> <th>Code</th> - <th>Category</th> + <th>iGEM parts</th> </tr> <tr> <td>RBS<span>34</span></td> @@ -134,7 +134,7 @@ <th>Function</th> <th>Construct</th> <th>Code</th> - <th>Category</th> + <th>iGEM parts</th> </tr> <tr> <td>Ter<span>L3S2P21</span></td> @@ -175,7 +175,7 @@ <th>Function</th> <th>Construct</th> <th>Code</th> - <th>Category</th> + <th>iGEM parts</th> </tr> <tr> <td>mer<span>Rm4-1</span></td> @@ -202,7 +202,7 @@ <th>Function</th> <th>Construct</th> <th>Code</th> - <th>Category</th> + <th>iGEM parts</th> </tr> <tr> <td>sfGFP<span>mChartreuse</span></td> @@ -229,7 +229,7 @@ <th>Function</th> <th>Construct</th> <th>Code</th> - <th>Category</th> + <th>iGEM parts</th> </tr> <tr> <td>OmpT<span>LOT</span></td> @@ -288,7 +288,7 @@ <th>Function</th> <th>Construct</th> <th>Code</th> - <th>Category</th> + <th>iGEM parts</th> </tr> <tr> <td>G4S<span>linker</span></td> @@ -352,7 +352,7 @@ <th>Function</th> <th>Construct</th> <th>Code</th> - <th>Category</th> + <th>iGEM parts</th> </tr> <tr> <td>NOT(TetR,TEV)-mRFP1magenta</td> diff --git a/wiki/pages/results.html b/wiki/pages/results.html index 4c95a74f6b28285be457d5ddb1eb32a693d51834..2a2359b898969b3707005de51e321e622ed6ce92 100644 --- a/wiki/pages/results.html +++ b/wiki/pages/results.html @@ -156,7 +156,7 @@ <p> We will validate our biosensors by determining the amount of organic mercury in real samples with concentrations already known by atomic absorption spectroscopy (AAS), we expect to obtain a high correlation - between both methods. We expect a high sensitivity of both strategies (əppb), because organic mercury is + between both methods. We expect a high sensitivity of both strategies (ppb), because organic mercury is more liposoluble than inorganic mercury. </p> <div class="content__results-magenta-reporter"> @@ -176,7 +176,7 @@ <p> We propose to deploy our biosensors in a portable, cheap and easy to use platform. To keep the bacteria alive - and active we will use lyophilization or alginate encapsulation (see lab-experiments), our biosensor will be + and active we will use lyophilization or alginate encapsulation (see <a href="https://2024.igem.wiki/bolivia/experiments">lab-experiments</a> ), our biosensor will be inside autoclavable glass tubes with a low volume requirement (1-2ml) of sample. </p> <p> @@ -190,7 +190,7 @@ of mercury present in the samples. The excitation wavelength of the mChartreuse is λex=487nm and the emission wavelength is λem=510nm, while mRFPmagenta has λex=588nm and λem=616nm. After data collection, the bacteria will be inactivated by taking them to the autoclave and will be followed as presented in “Waste and - Disinfection Protocol for the Mercury Biosensor Device†(Safety). + Disinfection Protocol for the Mercury Biosensor Device†(<a href="https://2024.igem.wiki/bolivia/safety">Safety</a> ). </p> <img data-aos="zoom-out-up" class="img-normal-results" src="https://static.igem.wiki/teams/5257/project/results/10-results.webp" alt=""> <p class="text__center-results">Sequence of steps to validate our biosensor in hardware.</p>