diff --git a/.output/nitro.json b/.output/nitro.json
index f773c4470a82e927e8384992f3f0eb057b316845..33000cd4e0431efa87c1e99431987f6b7228220d 100644
--- a/.output/nitro.json
+++ b/.output/nitro.json
@@ -1,5 +1,5 @@
 {
-  "date": "2024-10-02T11:36:35.801Z",
+  "date": "2024-10-02T12:11:32.841Z",
   "preset": "static",
   "framework": {
     "name": "nuxt",
diff --git a/.output/public/200.html b/.output/public/200.html
index c5e0d1bbcbbaf4d8be8b41fdbec1165e4ccc9dd6..6d32304c4564ec28a7d9e74bfa40f247649722d1 100644
--- a/.output/public/200.html
+++ b/.output/public/200.html
@@ -7,13 +7,13 @@
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20px;position:relative}.el-cascader-node.is-selectable.in-active-path{color:var(--el-cascader-menu-text-color)}.el-cascader-node.in-active-path,.el-cascader-node.is-active,.el-cascader-node.is-selectable.in-checked-path{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader-node:not(.is-disabled){cursor:pointer}.el-cascader-node:not(.is-disabled):focus,.el-cascader-node:not(.is-disabled):hover{background:var(--el-cascader-node-background-hover)}.el-cascader-node.is-disabled{color:var(--el-cascader-node-color-disabled);cursor:not-allowed}.el-cascader-node__prefix{left:10px;position:absolute}.el-cascader-node__postfix{position:absolute;right:10px}.el-cascader-node__label{flex:1;overflow:hidden;padding:0 8px;text-align:left;text-overflow:ellipsis;white-space:nowrap}.el-cascader-node>.el-checkbox,.el-cascader-node>.el-radio{margin-right:0}.el-cascader-node>.el-radio .el-radio__label{padding-left:0}.el-cascader{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);display:inline-block;font-size:var(--el-font-size-base);line-height:32px;outline:none;position:relative;vertical-align:middle}.el-cascader:not(.is-disabled):hover .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset;cursor:pointer}.el-cascader .el-input{cursor:pointer;display:flex}.el-cascader .el-input .el-input__inner{cursor:pointer;text-overflow:ellipsis}.el-cascader .el-input .el-input__suffix-inner .el-icon{height:calc(100% - 2px)}.el-cascader .el-input .el-input__suffix-inner .el-icon svg{vertical-align:middle}.el-cascader .el-input .icon-arrow-down{font-size:14px;transition:transform var(--el-transition-duration)}.el-cascader .el-input .icon-arrow-down.is-reverse{transform:rotate(180deg)}.el-cascader .el-input .icon-circle-close:hover{color:var(--el-input-clear-hover-color,var(--el-text-color-secondary))}.el-cascader .el-input.is-focus .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-focus-border-color,var(--el-color-primary)) inset}.el-cascader--large{font-size:14px;line-height:40px}.el-cascader--small{font-size:12px;line-height:24px}.el-cascader.is-disabled .el-cascader__label{color:var(--el-disabled-text-color);z-index:calc(var(--el-index-normal) + 1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper .el-popper__arrow:before{border:var(--el-cascader-menu-border)}.el-cascader__dropdown.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper{box-shadow:var(--el-cascader-menu-shadow)}.el-cascader__tags{box-sizing:border-box;display:flex;flex-wrap:wrap;left:0;line-height:normal;position:absolute;right:30px;text-align:left;top:50%;transform:translateY(-50%)}.el-cascader__tags .el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags .el-tag{align-items:center;background:var(--el-fill-color);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag.el-tag--dark,.el-cascader__collapse-tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__collapse-tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__collapse-tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__collapse-tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__suggestion-panel{border-radius:var(--el-cascader-menu-radius)}.el-cascader__suggestion-list{color:var(--el-cascader-menu-text-color);font-size:var(--el-font-size-base);margin:0;max-height:204px;padding:6px 0;text-align:center}.el-cascader__suggestion-item{align-items:center;cursor:pointer;display:flex;height:34px;justify-content:space-between;outline:none;padding:0 15px;text-align:left}.el-cascader__suggestion-item:focus,.el-cascader__suggestion-item:hover{background:var(--el-cascader-node-background-hover)}.el-cascader__suggestion-item.is-checked{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader__suggestion-item>span{margin-right:10px}.el-cascader__empty-text{color:var(--el-cascader-color-empty);margin:10px 0}.el-cascader__search-input{background:transparent;border:none;box-sizing:border-box;color:var(--el-cascader-menu-text-color);flex:1;height:24px;margin:2px 0 2px 11px;min-width:60px;outline:none;padding:0}.el-cascader__search-input::-moz-placeholder{color:transparent}.el-cascader__search-input::placeholder{color:transparent}.el-check-tag{background-color:var(--el-color-info-light-9);border-radius:var(--el-border-radius-base);color:var(--el-color-info);cursor:pointer;display:inline-block;font-size:var(--el-font-size-base);font-weight:700;line-height:var(--el-font-size-base);padding:7px 15px;transition:var(--el-transition-all)}.el-check-tag:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--primary.is-checked{background-color:var(--el-color-primary-light-8);color:var(--el-color-primary)}.el-check-tag.el-check-tag--primary.is-checked:hover{background-color:var(--el-color-primary-light-7)}.el-check-tag.el-check-tag--primary.is-checked.is-disabled{background-color:var(--el-color-primary-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-checked.is-disabled:hover{background-color:var(--el-color-primary-light-8)}.el-check-tag.el-check-tag--primary.is-disabled{background-color:var(--el-color-info-light-9);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-check-tag.el-check-tag--success.is-checked{background-color:var(--el-color-success-light-8);color:var(--el-color-success)}.el-check-tag.el-check-tag--success.is-checked:hover{background-color:var(--el-color-success-light-7)}.el-check-tag.el-check-tag--success.is-checked.is-disabled{background-color:var(--el-color-success-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-checked.is-disabled:hover{background-color:var(--el-color-success-light-8)}.el-check-tag.el-check-tag--success.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-disabled,.el-check-tag.el-check-tag--success.is-disabled:hover{background-color:var(--el-color-success-light-9)}.el-check-tag.el-check-tag--warning.is-checked{background-color:var(--el-color-warning-light-8);color:var(--el-color-warning)}.el-check-tag.el-check-tag--warning.is-checked:hover{background-color:var(--el-color-warning-light-7)}.el-check-tag.el-check-tag--warning.is-checked.is-disabled{background-color:var(--el-color-warning-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-checked.is-disabled:hover{background-color:var(--el-color-warning-light-8)}.el-check-tag.el-check-tag--warning.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-disabled,.el-check-tag.el-check-tag--warning.is-disabled:hover{background-color:var(--el-color-warning-light-9)}.el-check-tag.el-check-tag--danger.is-checked{background-color:var(--el-color-danger-light-8);color:var(--el-color-danger)}.el-check-tag.el-check-tag--danger.is-checked:hover{background-color:var(--el-color-danger-light-7)}.el-check-tag.el-check-tag--danger.is-checked.is-disabled{background-color:var(--el-color-danger-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-checked.is-disabled:hover{background-color:var(--el-color-danger-light-8)}.el-check-tag.el-check-tag--danger.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-disabled,.el-check-tag.el-check-tag--danger.is-disabled:hover{background-color:var(--el-color-danger-light-9)}.el-check-tag.el-check-tag--error.is-checked{background-color:var(--el-color-error-light-8);color:var(--el-color-error)}.el-check-tag.el-check-tag--error.is-checked:hover{background-color:var(--el-color-error-light-7)}.el-check-tag.el-check-tag--error.is-checked.is-disabled{background-color:var(--el-color-error-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-checked.is-disabled:hover{background-color:var(--el-color-error-light-8)}.el-check-tag.el-check-tag--error.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-disabled,.el-check-tag.el-check-tag--error.is-disabled:hover{background-color:var(--el-color-error-light-9)}.el-check-tag.el-check-tag--info.is-checked{background-color:var(--el-color-info-light-8);color:var(--el-color-info)}.el-check-tag.el-check-tag--info.is-checked:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--info.is-checked.is-disabled{background-color:var(--el-color-info-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-checked.is-disabled:hover{background-color:var(--el-color-info-light-8)}.el-check-tag.el-check-tag--info.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-disabled,.el-check-tag.el-check-tag--info.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-checkbox-button{--el-checkbox-button-checked-bg-color:var(--el-color-primary);--el-checkbox-button-checked-text-color:var(--el-color-white);--el-checkbox-button-checked-border-color:var(--el-color-primary);display:inline-block;position:relative}.el-checkbox-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left-color:transparent;bord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15px;position:relative;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-checkbox-button__inner.is-round{padding:8px 15px}.el-checkbox-button__inner:hover{color:var(--el-color-primary)}.el-checkbox-button__inner [class*=el-icon-]{line-height:.9}.el-checkbox-button__inner [class*=el-icon-]+span{margin-left:5px}.el-checkbox-button__original{margin:0;opacity:0;outline:none;position:absolute;z-index:-1}.el-checkbox-button.is-checked .el-checkbox-button__inner{background-color:var(--el-checkbox-button-checked-bg-color);border-color:var(--el-checkbox-button-checked-border-color);box-shadow:-1px 0 0 0 var(--el-color-primary-light-7);color:var(--el-checkbox-button-checked-text-color)}.el-checkbox-button.is-checked:first-child .el-checkbox-button__inner{border-left-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button.is-disabled .el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large .el-checkbox-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-checkbox-button--large .el-checkbox-button__inner.is-round{padding:12px 19px}.el-checkbox-button--small .el-checkbox-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-checkbox-button--small .el-checkbox-button__inner.is-round{padding:5px 11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) .el-checkbox__original:not(:focus-visible){border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__input.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-checked-icon-color);content:"";display:block;height:2px;left:0;position:absolute;right:0;top:5px;transform:scale(.5)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:after{display:none}.el-checkbox__inner{background-color:var(--el-checkbox-bg-color);border:var(--el-checkbox-input-border);border-radius:var(--el-checkbox-border-radius);box-sizing:border-box;display:inline-block;height:var(--el-checkbox-input-height);position:relative;transition:border-color .25s cubic-bezier(.71,-.46,.29,1.46),background-color .25s cubic-bezier(.71,-.46,.29,1.46),outline .25s cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 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54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 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var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid 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var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker .el-picker-panel__content{margin:0}.el-date-range-picker__header{height:28px;position:relative;text-align:center}.el-date-range-picker__header [class*=arrow-left]{float:left}.el-date-range-picker__header [class*=arrow-right]{float:right}.el-date-range-picker__header div{font-size:16px;font-weight:500;margin-right:50px}.el-date-range-picker__content{box-sizing:border-box;float:left;margin:0;padding:16px;width:50%}.el-date-range-picker__content.is-left{border-right:1px solid var(--el-datepicker-inner-border-color)}.el-date-range-picker__content .el-date-range-picker__header div{margin-left:50px;margin-right:50px}.el-date-range-picker__editors-wrap{box-sizing:border-box;display:table-cell}.el-date-range-picker__editors-wrap.is-right{text-align:right}.el-date-range-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid var(--el-timepicker-inner-border-color,var(--el-border-color-light));box-sizing:border-box;height:36px;line-height:25px;padding:4px;text-align:right}.el-time-panel__btn{background-color:transparent;border:none;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:28px;margin:0 5px;outline:none;padding:0 5px}.el-time-panel__btn.confirm{color:var(--el-timepicker-active-color,var(--el-color-primary));font-weight:800}.el-descriptions{--el-descriptions-table-border:1px solid var(--el-border-color-lighter);--el-descriptions-item-bordered-label-background:var(--el-fill-color-light);box-sizing:border-box;color:var(--el-text-color-primary);font-size:var(--el-font-size-base)}.el-descriptions__header{align-items:center;display:flex;justify-content:space-between;margin-bottom:16px}.el-descriptions__title{color:var(--el-text-color-primary);font-size:16px;font-weight:700}.el-descriptions__body{background-color:var(--el-fill-color-blank)}.el-descriptions__body 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var(--el-menu-base-level-padding)}.horizontal-collapse-transition .el-sub-menu__title 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.el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small .el-radio__inner{height:12px;width:12px}.el-rate{--el-rate-height:20px;--el-rate-font-size:var(--el-font-size-base);--el-rate-icon-size:18px;--el-rate-icon-margin:6px;--el-rate-void-color:var(--el-border-color-darker);--el-rate-fill-color:#f7ba2a;--el-rate-disabled-void-color:var(--el-fill-color);--el-rate-text-color:var(--el-text-color-primary);align-items:center;display:inline-flex;height:32px}.el-rate:active,.el-rate:focus{outline:none}.el-rate__item{color:var(--el-rate-void-color);cursor:pointer;display:inline-block;font-size:0;line-height:normal;position:relative;vertical-align:middle}.el-rate .el-rate__icon{display:inline-block;font-size:var(--el-rate-icon-size);margin-right:var(--el-rate-icon-margin);position:relative;transition:var(--el-transition-duration)}.el-rate .el-rate__icon.hover{transform:scale(1.15)}.el-rate .el-rate__icon .path2{left:0;position:absolute;top:0}.el-rate .el-rate__icon.is-active{color:var(--el-rate-fill-color)}.el-rate__decimal{color:var(--el-rate-fill-color);display:inline-block;overflow:hidden}.el-rate__decimal,.el-rate__decimal--box{left:0;position:absolute;top:0}.el-rate__text{color:var(--el-rate-text-color);font-size:var(--el-rate-font-size);vertical-align:middle}.el-rate--large{height:40px}.el-rate--small{height:24px}.el-rate--small .el-rate__icon{font-size:14px}.el-rate.is-disabled .el-rate__item{color:var(--el-rate-disabled-void-color);cursor:auto}.el-result{--el-result-padding:40px 30px;--el-result-icon-font-size:64px;--el-result-title-font-size:20px;--el-result-title-margin-top:20px;--el-result-subtitle-margin-top:10px;--el-result-extra-margin-top:30px;align-items:center;box-sizing:border-box;display:flex;flex-direction:column;justify-content:center;padding:var(--el-result-padding);text-align:center}.el-result__icon svg{height:var(--el-result-icon-font-size);width:var(--el-result-icon-font-size)}.el-result__title{margin-top:var(--el-result-title-margin-top)}.el-result__title p{color:var(--el-text-color-primary);font-size:var(--el-result-title-font-size);line-height:1.3;margin:0}.el-result__subtitle{margin-top:var(--el-result-subtitle-margin-top)}.el-result__subtitle p{color:var(--el-text-color-regular);font-size:var(--el-font-size-base);line-height:1.3;margin:0}.el-result__extra{margin-top:var(--el-result-extra-margin-top)}.el-result .icon-primary{--el-result-color:var(--el-color-primary);color:var(--el-result-color)}.el-result .icon-success{--el-result-color:var(--el-color-success);color:var(--el-result-color)}.el-result .icon-warning{--el-result-color:var(--el-color-warning);color:var(--el-result-color)}.el-result .icon-danger{--el-result-color:var(--el-color-danger);color:var(--el-result-color)}.el-result .icon-error{--el-result-color:var(--el-color-error);color:var(--el-result-color)}.el-result 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.el-tag__close{color:var(--el-tag-text-color);flex-shrink:0}.el-tag .el-tag__close:hover{background-color:var(--el-tag-hover-color);color:var(--el-color-white)}.el-tag.el-tag--primary{--el-tag-text-color:var(--el-color-primary)}.el-tag.el-tag--success{--el-tag-text-color:var(--el-color-success)}.el-tag.el-tag--warning{--el-tag-text-color:var(--el-color-warning)}.el-tag.el-tag--danger{--el-tag-text-color:var(--el-color-danger)}.el-tag.el-tag--error{--el-tag-text-color:var(--el-color-error)}.el-tag.el-tag--info{--el-tag-text-color:var(--el-color-info)}.el-tag .el-icon{border-radius:50%;cursor:pointer;font-size:calc(var(--el-icon-size) - 2px);height:var(--el-icon-size);width:var(--el-icon-size)}.el-tag 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0;min-width:0}.time-select .el-picker-panel__content{margin:0;max-height:200px}.time-select-item{font-size:14px;line-height:20px;padding:8px 10px}.time-select-item.disabled{color:var(--el-datepicker-border-color);cursor:not-allowed}.time-select-item:hover{background-color:var(--el-fill-color-light);cursor:pointer;font-weight:700}.time-select .time-select-item.selected:not(.disabled){color:var(--el-color-primary);font-weight:700}.el-timeline-item{padding-bottom:20px;position:relative}.el-timeline-item__wrapper{padding-left:28px;position:relative;top:-3px}.el-timeline-item__tail{border-left:2px solid var(--el-timeline-node-color);height:100%;left:4px;position:absolute}.el-timeline-item .el-timeline-item__icon{color:var(--el-color-white);font-size:var(--el-font-size-small)}.el-timeline-item__node{align-items:center;background-color:var(--el-timeline-node-color);border-color:var(--el-timeline-node-color);border-radius:50%;box-sizing:border-box;display:flex;justify-content:center;position:absolute}.el-timeline-item__node--normal{height:var(--el-timeline-node-size-normal);left:-1px;width:var(--el-timeline-node-size-normal)}.el-timeline-item__node--large{height:var(--el-timeline-node-size-large);left:-2px;width:var(--el-timeline-node-size-large)}.el-timeline-item__node.is-hollow{background:var(--el-color-white);border-style:solid;border-width:2px}.el-timeline-item__node--primary{background-color:var(--el-color-primary);border-color:var(--el-color-primary)}.el-timeline-item__node--success{background-color:var(--el-color-success);border-color:var(--el-color-success)}.el-timeline-item__node--warning{background-color:var(--el-color-warning);border-color:var(--el-color-warning)}.el-timeline-item__node--danger{background-color:var(--el-color-danger);border-color:var(--el-color-danger)}.el-timeline-item__node--info{background-color:var(--el-color-info);border-color:var(--el-color-info)}.el-timeline-item__dot{align-items:center;display:flex;justify-content:center;position:absolute}.el-timeline-item__content{color:var(--el-text-color-primary)}.el-timeline-item__timestamp{color:var(--el-text-color-secondary);font-size:var(--el-font-size-small);line-height:1}.el-timeline-item__timestamp.is-top{margin-bottom:8px;padding-top:4px}.el-timeline-item__timestamp.is-bottom{margin-top:8px}.el-timeline{--el-timeline-node-size-normal:12px;--el-timeline-node-size-large:14px;--el-timeline-node-color:var(--el-border-color-light);font-size:var(--el-font-size-base);list-style:none;margin:0}.el-timeline 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var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);display:flex;font-size:var(--el-cascader-menu-font-size)}.el-cascader-panel.is-bordered{border:var(--el-cascader-menu-border);border-radius:var(--el-cascader-menu-radius)}.el-cascader-menu{border-right:var(--el-cascader-menu-border);box-sizing:border-box;color:var(--el-cascader-menu-text-color);min-width:180px}.el-cascader-menu:last-child{border-right:none}.el-cascader-menu:last-child .el-cascader-node{padding-right:20px}.el-cascader-menu__wrap.el-scrollbar__wrap{height:204px}.el-cascader-menu__list{box-sizing:border-box;list-style:none;margin:0;min-height:100%;padding:6px 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1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper 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.el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags 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15px;position:relative;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-checkbox-button__inner.is-round{padding:8px 15px}.el-checkbox-button__inner:hover{color:var(--el-color-primary)}.el-checkbox-button__inner [class*=el-icon-]{line-height:.9}.el-checkbox-button__inner [class*=el-icon-]+span{margin-left:5px}.el-checkbox-button__original{margin:0;opacity:0;outline:none;position:absolute;z-index:-1}.el-checkbox-button.is-checked .el-checkbox-button__inner{background-color:var(--el-checkbox-button-checked-bg-color);border-color:var(--el-checkbox-button-checked-border-color);box-shadow:-1px 0 0 0 var(--el-color-primary-light-7);color:var(--el-checkbox-button-checked-text-color)}.el-checkbox-button.is-checked:first-child .el-checkbox-button__inner{border-left-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button.is-disabled .el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large .el-checkbox-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-checkbox-button--large .el-checkbox-button__inner.is-round{padding:12px 19px}.el-checkbox-button--small .el-checkbox-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-checkbox-button--small .el-checkbox-button__inner.is-round{padding:5px 11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) .el-checkbox__original:not(:focus-visible){border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__input.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-checked-icon-color);content:"";display:block;height:2px;left:0;position:absolute;right:0;top:5px;transform:scale(.5)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:after{display:none}.el-checkbox__inner{background-color:var(--el-checkbox-bg-color);border:var(--el-checkbox-input-border);border-radius:var(--el-checkbox-border-radius);box-sizing:border-box;display:inline-block;height:var(--el-checkbox-input-height);position:relative;transition:border-color .25s cubic-bezier(.71,-.46,.29,1.46),background-color .25s cubic-bezier(.71,-.46,.29,1.46),outline .25s cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.el-checkbox--small .el-checkbox__input.is-indeterminate .el-checkbox__inner:before{top:4px}.el-checkbox.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox:last-of-type{margin-right:0}[class*=el-col-]{box-sizing:border-box}[class*=el-col-].is-guttered{display:block;min-height:1px}.el-col-0{flex:0 0 0%;max-width:0}.el-col-0,.el-col-0.is-guttered{display:none}.el-col-offset-0{margin-left:0}.el-col-pull-0{position:relative;right:0}.el-col-push-0{left:0;position:relative}.el-col-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-1,.el-col-1.is-guttered{display:block}.el-col-offset-1{margin-left:4.1666666667%}.el-col-pull-1{position:relative;right:4.1666666667%}.el-col-push-1{left:4.1666666667%;position:relative}.el-col-2{flex:0 0 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0 75%;max-width:75%}.el-col-18,.el-col-18.is-guttered{display:block}.el-col-offset-18{margin-left:75%}.el-col-pull-18{position:relative;right:75%}.el-col-push-18{left:75%;position:relative}.el-col-19{flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-19,.el-col-19.is-guttered{display:block}.el-col-offset-19{margin-left:79.1666666667%}.el-col-pull-19{position:relative;right:79.1666666667%}.el-col-push-19{left:79.1666666667%;position:relative}.el-col-20{flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-20,.el-col-20.is-guttered{display:block}.el-col-offset-20{margin-left:83.3333333333%}.el-col-pull-20{position:relative;right:83.3333333333%}.el-col-push-20{left:83.3333333333%;position:relative}.el-col-21{flex:0 0 87.5%;max-width:87.5%}.el-col-21,.el-col-21.is-guttered{display:block}.el-col-offset-21{margin-left:87.5%}.el-col-pull-21{position:relative;right:87.5%}.el-col-push-21{left:87.5%;position:relative}.el-col-22{flex:0 0 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16.6666666667%;max-width:16.6666666667%}.el-col-xs-4,.el-col-xs-4.is-guttered{display:block}.el-col-xs-offset-4{margin-left:16.6666666667%}.el-col-xs-pull-4{position:relative;right:16.6666666667%}.el-col-xs-push-4{left:16.6666666667%;position:relative}.el-col-xs-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-xs-5,.el-col-xs-5.is-guttered{display:block}.el-col-xs-offset-5{margin-left:20.8333333333%}.el-col-xs-pull-5{position:relative;right:20.8333333333%}.el-col-xs-push-5{left:20.8333333333%;position:relative}.el-col-xs-6{flex:0 0 25%;max-width:25%}.el-col-xs-6,.el-col-xs-6.is-guttered{display:block}.el-col-xs-offset-6{margin-left:25%}.el-col-xs-pull-6{position:relative;right:25%}.el-col-xs-push-6{left:25%;position:relative}.el-col-xs-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-xs-7,.el-col-xs-7.is-guttered{display:block}.el-col-xs-offset-7{margin-left:29.1666666667%}.el-col-xs-pull-7{position:relative;right:29.1666666667%}.el-col-xs-push-7{left:29.1666666667%;position:relative}.el-col-xs-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xs-8,.el-col-xs-8.is-guttered{display:block}.el-col-xs-offset-8{margin-left:33.3333333333%}.el-col-xs-pull-8{position:relative;right:33.3333333333%}.el-col-xs-push-8{left:33.3333333333%;position:relative}.el-col-xs-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xs-9,.el-col-xs-9.is-guttered{display:block}.el-col-xs-offset-9{margin-left:37.5%}.el-col-xs-pull-9{position:relative;right:37.5%}.el-col-xs-push-9{left:37.5%;position:relative}.el-col-xs-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-xs-10.is-guttered{display:block}.el-col-xs-offset-10{margin-left:41.6666666667%}.el-col-xs-pull-10{position:relative;right:41.6666666667%}.el-col-xs-push-10{left:41.6666666667%;position:relative}.el-col-xs-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-xs-11.is-guttered{display:block}.el-col-xs-offset-11{margin-left:45.8333333333%}.el-col-xs-pull-11{position:relative;right:45.8333333333%}.el-col-xs-push-11{left:45.8333333333%;position:relative}.el-col-xs-12{display:block;flex:0 0 50%;max-width:50%}.el-col-xs-12.is-guttered{display:block}.el-col-xs-offset-12{margin-left:50%}.el-col-xs-pull-12{position:relative;right:50%}.el-col-xs-push-12{left:50%;position:relative}.el-col-xs-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-xs-13.is-guttered{display:block}.el-col-xs-offset-13{margin-left:54.1666666667%}.el-col-xs-pull-13{position:relative;right:54.1666666667%}.el-col-xs-push-13{left:54.1666666667%;position:relative}.el-col-xs-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xs-14.is-guttered{display:block}.el-col-xs-offset-14{margin-left:58.3333333333%}.el-col-xs-pull-14{position:relative;right:58.3333333333%}.el-col-xs-push-14{left:58.3333333333%;position:relative}.el-col-xs-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xs-15.is-guttered{display:block}.el-col-xs-offset-15{margin-left:62.5%}.el-col-xs-pull-15{position:relative;right:62.5%}.el-col-xs-push-15{left:62.5%;position:relative}.el-col-xs-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xs-16.is-guttered{display:block}.el-col-xs-offset-16{margin-left:66.6666666667%}.el-col-xs-pull-16{position:relative;right:66.6666666667%}.el-col-xs-push-16{left:66.6666666667%;position:relative}.el-col-xs-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xs-17.is-guttered{display:block}.el-col-xs-offset-17{margin-left:70.8333333333%}.el-col-xs-pull-17{position:relative;right:70.8333333333%}.el-col-xs-push-17{left:70.8333333333%;position:relative}.el-col-xs-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xs-18.is-guttered{display:block}.el-col-xs-offset-18{margin-left:75%}.el-col-xs-pull-18{position:relative;right:75%}.el-col-xs-push-18{left:75%;position:relative}.el-col-xs-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xs-19.is-guttered{display:block}.el-col-xs-offset-19{margin-left:79.1666666667%}.el-col-xs-pull-19{position:relative;right:79.1666666667%}.el-col-xs-push-19{left:79.1666666667%;position:relative}.el-col-xs-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xs-20.is-guttered{display:block}.el-col-xs-offset-20{margin-left:83.3333333333%}.el-col-xs-pull-20{position:relative;right:83.3333333333%}.el-col-xs-push-20{left:83.3333333333%;position:relative}.el-col-xs-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xs-21.is-guttered{display:block}.el-col-xs-offset-21{margin-left:87.5%}.el-col-xs-pull-21{position:relative;right:87.5%}.el-col-xs-push-21{left:87.5%;position:relative}.el-col-xs-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 0%;max-width:0}.el-col-sm-0.is-guttered{display:none}.el-col-sm-offset-0{margin-left:0}.el-col-sm-pull-0{position:relative;right:0}.el-col-sm-push-0{left:0;position:relative}.el-col-sm-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-sm-1,.el-col-sm-1.is-guttered{display:block}.el-col-sm-offset-1{margin-left:4.1666666667%}.el-col-sm-pull-1{position:relative;right:4.1666666667%}.el-col-sm-push-1{left:4.1666666667%;position:relative}.el-col-sm-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-sm-2,.el-col-sm-2.is-guttered{display:block}.el-col-sm-offset-2{margin-left:8.3333333333%}.el-col-sm-pull-2{position:relative;right:8.3333333333%}.el-col-sm-push-2{left:8.3333333333%;position:relative}.el-col-sm-3{flex:0 0 12.5%;max-width:12.5%}.el-col-sm-3,.el-col-sm-3.is-guttered{display:block}.el-col-sm-offset-3{margin-left:12.5%}.el-col-sm-pull-3{position:relative;right:12.5%}.el-col-sm-push-3{left:12.5%;position:relative}.el-col-sm-4{flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 0%;max-width:0}.el-col-xl-0.is-guttered{display:none}.el-col-xl-offset-0{margin-left:0}.el-col-xl-pull-0{position:relative;right:0}.el-col-xl-push-0{left:0;position:relative}.el-col-xl-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-xl-1,.el-col-xl-1.is-guttered{display:block}.el-col-xl-offset-1{margin-left:4.1666666667%}.el-col-xl-pull-1{position:relative;right:4.1666666667%}.el-col-xl-push-1{left:4.1666666667%;position:relative}.el-col-xl-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-xl-2,.el-col-xl-2.is-guttered{display:block}.el-col-xl-offset-2{margin-left:8.3333333333%}.el-col-xl-pull-2{position:relative;right:8.3333333333%}.el-col-xl-push-2{left:8.3333333333%;position:relative}.el-col-xl-3{flex:0 0 12.5%;max-width:12.5%}.el-col-xl-3,.el-col-xl-3.is-guttered{display:block}.el-col-xl-offset-3{margin-left:12.5%}.el-col-xl-pull-3{position:relative;right:12.5%}.el-col-xl-push-3{left:12.5%;position:relative}.el-col-xl-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-xl-4,.el-col-xl-4.is-guttered{display:block}.el-col-xl-offset-4{margin-left:16.6666666667%}.el-col-xl-pull-4{position:relative;right:16.6666666667%}.el-col-xl-push-4{left:16.6666666667%;position:relative}.el-col-xl-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-xl-5,.el-col-xl-5.is-guttered{display:block}.el-col-xl-offset-5{margin-left:20.8333333333%}.el-col-xl-pull-5{position:relative;right:20.8333333333%}.el-col-xl-push-5{left:20.8333333333%;position:relative}.el-col-xl-6{flex:0 0 25%;max-width:25%}.el-col-xl-6,.el-col-xl-6.is-guttered{display:block}.el-col-xl-offset-6{margin-left:25%}.el-col-xl-pull-6{position:relative;right:25%}.el-col-xl-push-6{left:25%;position:relative}.el-col-xl-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-xl-10.is-guttered{display:block}.el-col-xl-offset-10{margin-left:41.6666666667%}.el-col-xl-pull-10{position:relative;right:41.6666666667%}.el-col-xl-push-10{left:41.6666666667%;position:relative}.el-col-xl-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-xl-11.is-guttered{display:block}.el-col-xl-offset-11{margin-left:45.8333333333%}.el-col-xl-pull-11{position:relative;right:45.8333333333%}.el-col-xl-push-11{left:45.8333333333%;position:relative}.el-col-xl-12{display:block;flex:0 0 50%;max-width:50%}.el-col-xl-12.is-guttered{display:block}.el-col-xl-offset-12{margin-left:50%}.el-col-xl-pull-12{position:relative;right:50%}.el-col-xl-push-12{left:50%;position:relative}.el-col-xl-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid 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12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range 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td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today 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var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td 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var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 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.el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small 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var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel 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.el-overlay-message-box{bottom:0;left:0;overflow:auto;padding:16px;position:fixed;right:0;text-align:center;top:0}.el-overlay.is-message-box .el-overlay-message-box:after{content:"";display:inline-block;height:100%;vertical-align:middle;width:0}.el-message-box.is-draggable .el-message-box__header{cursor:move;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-message-box__header{padding-bottom:var(--el-messagebox-padding-primary)}.el-message-box__header.show-close{padding-right:calc(var(--el-messagebox-padding-primary) + var(--el-message-close-size, 16px))}.el-message-box__title{color:var(--el-messagebox-title-color);font-size:var(--el-messagebox-font-size);line-height:var(--el-messagebox-font-line-height)}.el-message-box__headerbtn{background:transparent;border:none;cursor:pointer;font-size:var(--el-message-close-size,16px);height:40px;outline:none;padding:0;position:absolute;right:0;top:0;width:40px}.el-message-box__headerbtn 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msgbox-fade-in{0%{opacity:0;transform:translate3d(0,-20px,0)}to{opacity:1;transform:translateZ(0)}}.el-message{--el-message-bg-color:var(--el-color-info-light-9);--el-message-border-color:var(--el-border-color-lighter);--el-message-padding:11px 15px;--el-message-close-size:16px;--el-message-close-icon-color:var(--el-text-color-placeholder);--el-message-close-hover-color:var(--el-text-color-secondary);align-items:center;background-color:var(--el-message-bg-color);border-color:var(--el-message-border-color);border-radius:var(--el-border-radius-base);border-style:var(--el-border-style);border-width:var(--el-border-width);box-sizing:border-box;display:flex;gap:8px;left:50%;max-width:calc(100% - 32px);padding:var(--el-message-padding);position:fixed;top:20px;transform:translateX(-50%);transition:opacity var(--el-transition-duration),transform .4s,top 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var(--el-notification-border-color);border-radius:var(--el-notification-radius);box-shadow:var(--el-notification-shadow);box-sizing:border-box;display:flex;overflow:hidden;overflow-wrap:break-word;padding:var(--el-notification-padding);position:fixed;transition:opacity var(--el-transition-duration),transform var(--el-transition-duration),left var(--el-transition-duration),right var(--el-transition-duration),top .4s,bottom var(--el-transition-duration);width:var(--el-notification-width);z-index:9999}.el-notification.right{right:16px}.el-notification.left{left:16px}.el-notification__group{margin-left:var(--el-notification-group-margin-left);margin-right:var(--el-notification-group-margin-right)}.el-notification__title{color:var(--el-notification-title-color);font-size:var(--el-notification-title-font-size);font-weight:700;line-height:var(--el-notification-icon-size);margin:0}.el-notification__content{color:var(--el-notification-content-color);font-size:var(--el-notification-content-font-size);line-height:24px;margin:6px 0 0}.el-notification__content p{margin:0}.el-notification .el-notification__icon{font-size:var(--el-notification-icon-size);height:var(--el-notification-icon-size);width:var(--el-notification-icon-size)}.el-notification 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.el-notification--error{--el-notification-icon-color:var(--el-color-error);color:var(--el-notification-icon-color)}.el-notification-fade-enter-from.right{right:0;transform:translateX(100%)}.el-notification-fade-enter-from.left{left:0;transform:translateX(-100%)}.el-notification-fade-leave-to{opacity:0}.el-overlay{background-color:var(--el-overlay-color-lighter);bottom:0;height:100%;left:0;overflow:auto;position:fixed;right:0;top:0;z-index:2000}.el-overlay .el-overlay-root{height:0}.el-page-header.is-contentful .el-page-header__main{border-top:1px solid var(--el-border-color-light);margin-top:16px}.el-page-header__header{align-items:center;display:flex;justify-content:space-between;line-height:24px}.el-page-header__left{align-items:center;display:flex;margin-right:40px;position:relative}.el-page-header__back{align-items:center;cursor:pointer;display:flex}.el-page-header__left .el-divider--vertical{margin:0 16px}.el-page-header__icon{align-items:center;display:flex;font-size:16px;margin-right:10px}.el-page-header__icon 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.el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large 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li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text 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1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus 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.el-rate__icon.is-active{color:var(--el-rate-fill-color)}.el-rate__decimal{color:var(--el-rate-fill-color);display:inline-block;overflow:hidden}.el-rate__decimal,.el-rate__decimal--box{left:0;position:absolute;top:0}.el-rate__text{color:var(--el-rate-text-color);font-size:var(--el-rate-font-size);vertical-align:middle}.el-rate--large{height:40px}.el-rate--small{height:24px}.el-rate--small .el-rate__icon{font-size:14px}.el-rate.is-disabled .el-rate__item{color:var(--el-rate-disabled-void-color);cursor:auto}.el-result{--el-result-padding:40px 30px;--el-result-icon-font-size:64px;--el-result-title-font-size:20px;--el-result-title-margin-top:20px;--el-result-subtitle-margin-top:10px;--el-result-extra-margin-top:30px;align-items:center;box-sizing:border-box;display:flex;flex-direction:column;justify-content:center;padding:var(--el-result-padding);text-align:center}.el-result__icon svg{height:var(--el-result-icon-font-size);width:var(--el-result-icon-font-size)}.el-result__title{margin-top:var(--el-result-title-margin-top)}.el-result__title p{color:var(--el-text-color-primary);font-size:var(--el-result-title-font-size);line-height:1.3;margin:0}.el-result__subtitle{margin-top:var(--el-result-subtitle-margin-top)}.el-result__subtitle p{color:var(--el-text-color-regular);font-size:var(--el-font-size-base);line-height:1.3;margin:0}.el-result__extra{margin-top:var(--el-result-extra-margin-top)}.el-result .icon-primary{--el-result-color:var(--el-color-primary);color:var(--el-result-color)}.el-result .icon-success{--el-result-color:var(--el-color-success);color:var(--el-result-color)}.el-result .icon-warning{--el-result-color:var(--el-color-warning);color:var(--el-result-color)}.el-result .icon-danger{--el-result-color:var(--el-color-danger);color:var(--el-result-color)}.el-result .icon-error{--el-result-color:var(--el-color-error);color:var(--el-result-color)}.el-result 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background-color;width:0}.el-scrollbar__thumb:hover{background-color:var(--el-scrollbar-hover-bg-color,var(--el-text-color-secondary));opacity:var(--el-scrollbar-hover-opacity,.5)}.el-scrollbar__bar{border-radius:4px;bottom:2px;position:absolute;right:2px;z-index:1}.el-scrollbar__bar.is-vertical{top:2px;width:6px}.el-scrollbar__bar.is-vertical>div{width:100%}.el-scrollbar__bar.is-horizontal{height:6px;left:2px}.el-scrollbar__bar.is-horizontal>div{height:100%}.el-scrollbar-fade-enter-active{transition:opacity .34s ease-out}.el-scrollbar-fade-leave-active{transition:opacity .12s ease-out}.el-scrollbar-fade-enter-from,.el-scrollbar-fade-leave-active{opacity:0}.el-select-dropdown{border-radius:var(--el-border-radius-base);box-sizing:border-box;z-index:calc(var(--el-index-top) + 1)}.el-select-dropdown .el-scrollbar.is-empty .el-select-dropdown__list{padding:0}.el-select-dropdown__empty,.el-select-dropdown__loading{color:var(--el-text-color-secondary);font-size:var(--el-select-font-size);margin:0;padding:10px 0;text-align:center}.el-select-dropdown__wrap{max-height:274px}.el-select-dropdown__list{box-sizing:border-box;list-style:none;margin:0;padding:6px 0}.el-select-dropdown__list.el-vl__window{margin:6px 0;padding:0}.el-select-dropdown__header{border-bottom:1px solid var(--el-border-color-light);padding:10px}.el-select-dropdown__footer{border-top:1px solid var(--el-border-color-light);padding:10px}.el-select-dropdown__item{box-sizing:border-box;color:var(--el-text-color-regular);cursor:pointer;font-size:var(--el-font-size-base);height:34px;line-height:34px;overflow:hidden;padding:0 32px 0 20px;position:relative;text-overflow:ellipsis;white-space:nowrap}.el-select-dropdown__item.is-hovering{background-color:var(--el-fill-color-light)}.el-select-dropdown__item.is-selected{color:var(--el-color-primary);font-weight:700}.el-select-dropdown__item.is-disabled{background-color:unset;color:var(--el-text-color-placeholder);cursor:not-allowed}.el-select-dropdown.is-multiple .el-select-dropdown__item.is-selected:after{background-color:var(--el-color-primary);background-position:50%;background-repeat:no-repeat;border-right:none;border-top:none;content:"";height:12px;mask:url("data:image/svg+xml;utf8,%3Csvg class='icon' width='200' height='200' viewBox='0 0 1024 1024' xmlns='http://www.w3.org/2000/svg'%3E%3Cpath fill='currentColor' d='M406.656 706.944L195.84 496.256a32 32 0 10-45.248 45.248l256 256 512-512a32 32 0 00-45.248-45.248L406.592 706.944z'%3E%3C/path%3E%3C/svg%3E") no-repeat;mask-size:100% 100%;-webkit-mask:url("data:image/svg+xml;utf8,%3Csvg class='icon' width='200' 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.el-select-dropdown__item{padding-left:20px}.el-select{--el-select-border-color-hover:var(--el-border-color-hover);--el-select-disabled-color:var(--el-disabled-text-color);--el-select-disabled-border:var(--el-disabled-border-color);--el-select-font-size:var(--el-font-size-base);--el-select-close-hover-color:var(--el-text-color-secondary);--el-select-input-color:var(--el-text-color-placeholder);--el-select-multiple-input-color:var(--el-text-color-regular);--el-select-input-focus-border-color:var(--el-color-primary);--el-select-input-font-size:14px;--el-select-width:100%;display:inline-block;position:relative;vertical-align:middle;width:var(--el-select-width)}.el-select__wrapper{align-items:center;background-color:var(--el-fill-color-blank);border-radius:var(--el-border-radius-base);box-shadow:0 0 0 1px var(--el-border-color) inset;box-sizing:border-box;cursor:pointer;display:flex;font-size:14px;gap:6px;line-height:24px;min-height:32px;padding:4px 12px;position:relative;text-align:left;transform:translateZ(0);transition:var(--el-transition-duration)}.el-select__wrapper.is-filterable{cursor:text}.el-select__wrapper.is-focused{box-shadow:0 0 0 1px var(--el-color-primary) inset}.el-select__wrapper.is-hovering:not(.is-focused){box-shadow:0 0 0 1px var(--el-border-color-hover) inset}.el-select__wrapper.is-disabled{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-select__wrapper.is-disabled,.el-select__wrapper.is-disabled:hover{box-shadow:0 0 0 1px var(--el-select-disabled-border) inset}.el-select__wrapper.is-disabled.is-focus{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-select__wrapper.is-disabled .el-select__selected-item{color:var(--el-select-disabled-color)}.el-select__wrapper.is-disabled .el-select__caret,.el-select__wrapper.is-disabled .el-tag{cursor:not-allowed}.el-select__prefix,.el-select__suffix{align-items:center;color:var(--el-input-icon-color,var(--el-text-color-placeholder));display:flex;flex-shrink:0;gap:6px}.el-select__caret{color:var(--el-select-input-color);cursor:pointer;font-size:var(--el-select-input-font-size);transform:rotate(0deg);transition:var(--el-transition-duration)}.el-select__caret.is-reverse{transform:rotate(180deg)}.el-select__selection{align-items:center;display:flex;flex:1;flex-wrap:wrap;gap:6px;min-width:0;position:relative}.el-select__selection.is-near{margin-left:-8px}.el-select__selection .el-tag{border-color:transparent;cursor:pointer}.el-select__selection .el-tag.el-tag--plain{border-color:var(--el-tag-border-color)}.el-select__selection .el-tag .el-tag__content{min-width:0}.el-select__selected-item{display:flex;flex-wrap:wrap;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-select__tags-text{line-height:normal}.el-select__placeholder,.el-select__tags-text{display:block;overflow:hidden;text-overflow:ellipsis;white-space:nowrap}.el-select__placeholder{color:var(--el-input-text-color,var(--el-text-color-regular));position:absolute;top:50%;transform:translateY(-50%);width:100%}.el-select__placeholder.is-transparent{color:var(--el-text-color-placeholder);-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-select__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-select__popper.el-popper,.el-select__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-border-color-light)}.el-select__popper.el-popper[data-popper-placement^=top] 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-{"generatedAt":1727868994372,"generateTime":640,"contents":[{"_path":"/drylab-hardware","_dir":"","_draft":false,"_partial":false,"_locale":"","title":"Hardware","description":"This year, the core objective of the BIT team is to develop a initiative detection system for EDCs in aquatic environments based on E.coli biosensors. Traditional detection methods, such as chromatography and immunoassays, require cumbersome procedures like sampling, pre-treatment, and laboratory examination, in addition to expensive laboratory equipment (e.g., chromatographs, microplate reader) and professional experimental techniques. These methods are time-consuming and costly.","body":{"type":"root","children":[{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"This year, the core objective of the BIT team is to develop "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"a initiative detection system for EDCs in aquatic environments based on E.coli biosensors."}]},{"type":"text","value":" Traditional detection methods, such as chromatography and immunoassays, require cumbersome procedures like sampling, pre-treatment, and laboratory examination, in addition to expensive laboratory equipment (e.g., chromatographs, microplate reader) and professional experimental techniques. These methods are time-consuming and costly."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"As EDCs continue to threaten aquatic environments and the quality of drinking water for residents, it is crucial to achieve portable detection and rapid response to EDCs. To meet the requirement, our goal is to develop a low costing, highly integrated, compact, portable, and user-friendly hardware platform that can be combined with smartphone software to automate the cultivation of engineering bacteria and the analysis of fluorescence "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"detection"}]},{"type":"text","value":"."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To ensure that the hardware device truly meets the needs of system construction, we have adopted a typical engineering and project design approach, namely: "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"demand analysis, scheme proposal, feasibility verification, feedback , system improvement, and goal achievement."}]},{"type":"text","value":" This process is an iterative one, and after several cycles, it ultimately meets all the needs and objectives."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/1.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 1 Hardware Design Flowchart"}]}]},{"type":"element","tag":"h2","props":{"id":"demand-analysis"},"children":[{"type":"text","value":"Demand Analysis"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To ensure the stability and accuracy of "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"fluorescence detection"}]},{"type":"text","value":", it is necessary to standardize the detection process and use high sensitive detection units."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Standardization of the detection process requires a continuous and stable chemical and physical environment during the resuscitation of engineering bacteria and fluorescence detection. The standardization of the chemical environment is mainly ensured by the quantified operation of experimental steps (including control of sample volume and the number of engineering bacteria, control of the chemical composition and concentration of the culture medium), and the standardization of the physical environment is mainly ensured by the stability of light source, temperature, and humidity. Among them, light exposure problem has been solved by the sealed and light-proof nature of the device, and the humidity in the sealed environment is mainly determined by temperature. The standardization of the detection process mainly aims to achieve "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"constant temperature control"}]},{"type":"text","value":" of the hardware device."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Since the concentration of "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"EDCs"}]},{"type":"text","value":" in the detection samples is very low，which also refer to the low intensity of fluorescence, coupled with the portability requirements of the equipment, the detection method required must be high sensitive and can be integrated into small devices. Common chemical detection methods cannot meet the requirements, so adopting a more sensitive, portable, and efficient detection method is crucial for the realization of our POCT system."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In order to enable smartphone software to automate the control of hardware devices and visualize the detection results, the construction of hardware devices needs to consider the transmission of data between software and hardware."}]},{"type":"element","tag":"h2","props":{"id":"scheme-proposal"},"children":[{"type":"text","value":"Scheme Proposal"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/2.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 2 First Edition Design Scheme"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/3.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 3 Circuit Diagram"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In the construction of the "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"temperature control module"}]},{"type":"text","value":", we employed an NTC thermistor as the "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"temperature sensor"}]},{"type":"text","value":", utilizing heating FILM and fan as the heat and cold sources respectively. To achieve "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"PID regulation"}]},{"type":"text","value":" for "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"temperature sensing and control"}]},{"type":"text","value":", we used the PWM wave output from the "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"TB6612FNG motor drive"}]},{"type":"text","value":" to adjust the power of the fan and heating FILM, thereby maintaining dynamic temperature stability."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"For the selection of the detection scheme, we comprehensively considered the detection accuracy and the portability of the device, ultimately choosing the "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"fluorescence detection module"}]},{"type":"text","value":". We initially selected the AS7341 as "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"fluorescence sensor"}]},{"type":"text","value":" "},{"type":"element","tag":"span","props":{},"children":[{"type":"text","value":"1"}]},{"type":"text","value":". Concurrently, to ensure consistent fluorescence generation, we redesigned and optimized the optical path."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"After the marked fluorescence is excited by a blue LED, the fluorescence sensor collect and record real-time fluorescence signals, which can be visualized on the mobile software via a "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"communication module"}]},{"type":"text","value":"."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"For the communication module, we used the HC-05 Bluetooth module to connect the hardware system with the mobile software, enabling real-time monitoring of the hardware device's operating status and feedback of the detection results."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In the initial version of the scheme, we used an STM32 chip as the "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"microcontroller Unit(MCU)"}]},{"type":"text","value":", connecting temperature control module, fluorescence detection module, and Bluetooth communication module, ensuring the normal operation of the device through programming instructions."}]},{"type":"element","tag":"mdc-wrap","props":{":class":"table-fig"},"children":[{"type":"element","tag":"table","props":{},"children":[{"type":"element","tag":"thead","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"th","props":{"align":"center"},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/4.png"},"children":[]}]},{"type":"element","tag":"th","props":{"align":"center"},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/5.png"},"children":[]}]}]}]},{"type":"element","tag":"tbody","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{"align":"center"},"children":[{"type":"text","value":"Figure 5:  First Edition Implementation Scheme"}]},{"type":"element","tag":"td","props":{"align":"center"},"children":[{"type":"text","value":"Figure 6: First Edition Equipment Model Diagram"}]}]}]}]}]},{"type":"element","tag":"h3","props":{"id":"feasibility-verification"},"children":[{"type":"text","value":"Feasibility Verification"}]},{"type":"element","tag":"h3","props":{"id":"temperature-stability-verification"},"children":[{"type":"text","value":"Temperature Stability Verification"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To verify the constant temperature effect of the temperature control system, we designed a temperature stability test experiment. When the temperature first reached 37°C, the temperature was recorded every 10 seconds for an additional 60 seconds. From the results shown in Figure 6, it can be concluded that the absolute difference between the average temperature and the set temperature was no greater than 1.1°C, and the temperature curve consistently fluctuated around 37°C. This indicates that the temperature control system can achieve dynamic temperature regulation, capable of maintaining the temperature close to 37°C. However, this temperature control method generates relatively large temperature fluctuations."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["flex","gap-4"]},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/6.png"},"children":[]},{"type":"text","value":" "},{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/7.png"},"children":[]}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 7 Measurement Results of  Initial Scheme's Temperature Stability"}]}]},{"type":"element","tag":"h3","props":{"id":"gradient-fluoresce-in-sodium-fluorescence-detection-verification"},"children":[{"type":"text","value":"Gradient Fluoresce in Sodium Fluorescence Detection Verification"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The AS7341 fluorescence sensor, by integrating filters of different wavelength bands, achieves the function of detecting light intensity (Lux) at specific wavelengths, which is equivalent to detecting the corresponding fluorescence intensity. To verify whether this sensor can correctly reflect the signal from the sample, we designed an experiment to explore the relationship between the concentration gradient of fluoresce in sodium and Lux. We prepared a series of fluoresce in sodium solutions with different gradients in a 2mL centrifuge tube for signal collection using the AS734. The results shown in Figure 8 indicate that the measured Lux values have a good linear correlation with the concentration gradient of fluoresce in sodium, demonstrating that our fluorescence detection module can accurately reflect the concentration of substances and is capable of conducting fluorescence detection."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/8.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 8 Initial Scheme's Fluorescence Verification Fitting Curve"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The above feasibility verification demonstrates that the temperature control module and fluorescence detection module designed in the first version of the scheme can meet the design requirements and are feasible."}]},{"type":"element","tag":"h2","props":{"id":"feedback"},"children":[{"type":"text","value":"Feedback"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We engaged in in-depth discussions with the biology team, design team, and professional instructors regarding our hardware design, obtaining extremely valuable feedback. This feedback is instrumental in guiding our further improvements."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"1.MCU:"}]},{"type":"text","value":" The STM32 requires an external wireless communication module to implement Bluetooth functionality, which increases the complexity of the design."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"2.Temperature Control Module:"}]},{"type":"text","value":" Using the TB6612FNG to drive temperature control presents issues with heat dissipation and difficulties in achieving integration."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"3.Detection Module:"}]},{"type":"text","value":" The scheme of combining a fluorescence sensor with an optical path tends to have fluctuations in the excitation light source, and the optical path alignment is challenging and susceptible to environmental interference."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"4.Biological Requirements:"}]},{"type":"text","value":" The current design only allows for single detection, and frequent changes in the "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"reaction tube"}]},{"type":"text","value":" can affect the stability of the detection environment, ultimately leading to inaccurate results."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In response to the issues raised and the suggestions from the instructors, we have made improvements based on the original design."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/9.png"},"children":[]}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 9 Improved Design Scheme"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Firstly, we replaced the STM32F103C8T6 with the Arduino Nano ESP32 chip as the MCU. The ESP32 features built-in Bluetooth communication capabilities and a rich set of peripheral interfaces. It supports the CMake and Ninja build systems, which will greatly simplify the design complexity. Its widespread adoption globally will also make it more universal and laying a foundation for future teams looking to design similar devices. For temperature control and detection, we replaced the original scheme with the TCM1040 temperature control module and the FM-4A digital fluorescence detection module from LIDE Technology, respectively. Compared to the original scheme, these modules offer more integrated features, further enhancing the accuracy and stability of the platform."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/10.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 10 Improved Design Circuit Diagram"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To meet the demand for high-throughput detection, we proposed two solutions: a microfluidic chip rotation detection platform and a linear slide rail detection platform. After modeling analysis and feasibility analysis, we ultimately chose the linear slide rail detection platform and designed a brand-new enclosure for it."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/11.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 11 Implementation Plan of the New Equipment"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/engineering-success/new.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 12 Model Diagram of the New Equipment"}]}]},{"type":"element","tag":"h3","props":{"id":"goal-achievement"},"children":[{"type":"text","value":"Goal Achievement"}]},{"type":"element","tag":"h4","props":{"id":"final-product"},"children":[{"type":"text","value":"Final Product"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The final system is primarily divided into a "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"sampling area"}]},{"type":"text","value":" and a "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"detection area."}]},{"type":"text","value":" In actual use, the freeze-dried engineering bacteria will be mixed with multiple samples to be tested in separate reaction chambers for cultivation, awaiting detection.Upon receiving operational commands from the software, the device rapidly heats the aluminum alloy temperature-controlled slot in "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"the sampling area"}]},{"type":"text","value":" and maintains a constant temperature, while also providing real-time feedback of the temperature and the operational status of the system to the mobile software interface via Bluetooth. Concurrently, the fluorescence detection module in the detection area begins to sequentially detect the fluorescence signals of each reaction chamber along the slide rail and provides real-time feedback to the software's visual interface. When the engineering bacteria grow to a certain extent, the intensity of the fluorescence signal will reflect the degree of endocrine disruptor effects."}]},{"type":"element","tag":"h5","props":{"id":"functional-testing"},"children":[{"type":"text","value":"Functional Testing"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Temperature Control Capability Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We conducted another temperature stability test on the latest version of the system. The results shown in Figure 12 indicate that the absolute difference between the average temperature and the set temperature is less than 0.3°C, suggesting that the stability of the improved temperature control system has been further enhanced. This can well meet practical requirements."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["flex","gap-4"]},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/13.png"},"children":[]},{"type":"text","value":" "},{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/14.png"},"children":[]}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 13 Measurement Results of Temperature Fluctuation"}]}]},{"type":"element","tag":"h5","props":{"id":"gradient-fluorescein-sodium-fluorescence-detection-verification"},"children":[{"type":"text","value":"Gradient Fluorescein Sodium Fluorescence Detection Verification"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The fluorescence validation test of the latest equipment shows that the tested Relative Fluorescence Units (RFU) values have a good linear relationship with the concentration gradient of fluorescein sodium. This indicates that the new fluorescence detection module can effectively reflect the concentration of substances, meeting the usage requirements."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/15.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 14 Fluorescence Verification Experiment Fitting Curve"}]}]},{"type":"element","tag":"h2","props":{"id":"android-software"},"children":[{"type":"text","value":"Android Software"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We have conducted functional testing on the already designed software. Initially, the software is imported onto an Android phone and the Bluetooth module is connected to check for a normal connection. The software is then opened to inspect whether the UI (User Interface) design is complete. Subsequently, the signals received from the module are used to test the graph plotting functionality to ensure it operates correctly, and the system is optimized based on the results."}]},{"type":"element","tag":"h2","props":{"id":"data-reception-verification"},"children":[{"type":"text","value":"Data Reception Verification"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Signals for initiation are transmitted from the mobile phone to the MCU via Bluetooth. As can be seen from the diagram, the hardware successfully starts up and returns a designated identifier."}]},{"type":"element","tag":"h2","props":{"id":"data-plotting-verification"},"children":[{"type":"text","value":"Data Plotting Verification"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"During operation, the MCU sends data to the mobile phone via Bluetooth. The mobile phone then parses this data and plots graphs of time-temperature and time-fluorescence intensity. The diagrams indicate that the program has successfully implemented its functionalities."}]},{"type":"element","tag":"h2","props":{"id":"improvement"},"children":[{"type":"text","value":"Improvement"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Due to the addition of a linear slide rail for the movement of fluorescence detection in the second version of the hardware design, the signals obtained from the fluorescence detection have also changed. Therefore, the program is required to implement the ability to distinguish between the fluorescence intensity pulse peaks of different channels and to obtain their respective maximum values (theoretically, the fluorescence intensity at the bottom of the tube) for plotting."}]},{"type":"element","tag":"h2","props":{"id":"array-splitting-and-plotting-verification"},"children":[{"type":"text","value":"Array Splitting and Plotting Verification"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We can observe a set of data with multiple pulse peaks returned by the MCU. The software successfully splits this data into several different arrays and obtains the maximum value from each array for plotting."}]},{"type":"element","tag":"h2","props":{"id":"summary"},"children":[{"type":"text","value":"Summary"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The final product and its testing demonstrate that the proactive detection system for EDCs we have developed is compact, portable, and user-friendly. It effectively controls the standardization of cultivation conditions, enabling rapid screening and assessment of EDCs in wastewater, significantly reducing reliance on laboratory equipment and professional personnel. Additionally, the system's high-throughput operation allows for the simultaneous detection of multiple samples, thereby conducting sample testing more efficiently."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In designing the hardware, we employed engineering and project design approaches, taking into account target requirements and user feedback. Our instructor, Professor Li Xiaoqiong, recommended replacing the STM32 chip with the ESP32 chip, which will aid in enhancing the readability and versatility of the design. During testing and actual use, we collected feedback from operators and utilized a high-throughput platform, facilitating users to test multiple samples conveniently."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To facilitate user operation, we have added detailed user instructions in the App and will record demonstration videos for ease of use. At the same time, we have organized detailed system design blueprints and modeling files, as well as detailed component selection and manufacturing methods. You can contact us on the contribution page to obtain the relevant documents, thereby assisting other participants in replicating our device. Our main controller and major systems utilize common integrated units, while other parts are made using industrial processing and 3D printing techniques, making them easy to replicate for reference and implementation by other teams."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["text-2xl"]},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"text","value":"References"}]}]}]},{"type":"element","tag":"mdc-wrap","props":{":class":"reference"},"children":[{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Wang Y, Chen H, Lin K, et al. Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device"},{"type":"element","tag":"span","props":{},"children":[{"type":"text","value":"j"}]},{"type":"text","value":". Nature Communications, 2024, 15(1):3279."}]}]}]}],"toc":{"title":"","searchDepth":1,"depth":3,"links":[{"id":"demand-analysis","depth":2,"text":"Demand Analysis"},{"id":"scheme-proposal","depth":2,"text":"Scheme Proposal","children":[{"id":"feasibility-verification","depth":3,"text":"Feasibility Verification"},{"id":"temperature-stability-verification","depth":3,"text":"Temperature Stability Verification"},{"id":"gradient-fluoresce-in-sodium-fluorescence-detection-verification","depth":3,"text":"Gradient Fluoresce in Sodium Fluorescence Detection Verification"}]},{"id":"feedback","depth":2,"text":"Feedback","children":[{"id":"goal-achievement","depth":3,"text":"Goal Achievement","children":[{"id":"final-product","depth":4,"text":"Final Product"}]}]},{"id":"android-software","depth":2,"text":"Android Software"},{"id":"data-reception-verification","depth":2,"text":"Data Reception Verification"},{"id":"data-plotting-verification","depth":2,"text":"Data Plotting Verification"},{"id":"improvement","depth":2,"text":"Improvement"},{"id":"array-splitting-and-plotting-verification","depth":2,"text":"Array Splitting and Plotting Verification"},{"id":"summary","depth":2,"text":"Summary"}]}},"_type":"markdown","_id":"content:drylab-hardware.md","_source":"content","_file":"drylab-hardware.md","_stem":"drylab-hardware","_extension":"md"},{"_path":"/hp-ihp","_dir":"","_draft":false,"_partial":false,"_locale":"","title":"Integrated Human Practice","description":"","body":{"type":"root","children":[{"type":"element","tag":"h2","props":{"id":"overview"},"children":[{"type":"text","value":"Overview"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The core of HP is to present project designs and products to the community and stakeholders, get feedback, integrate feedback into the project, and constantly adjust and modify the project according to the guidance of the feedback. This year, we proposed the \"BRIEF\" periodic iteration method to complete IHP. The aim is to ensure that projects meet practical needs, continuously improve project performance through feedback, meet the needs of stakeholders, and contribute to society in order to build projects that are good and responsible for the world."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/ihp/brief.jpg"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The \"BRIEF\" concept consists of five phases of IHP implementation, They are Brainstorming, Researching, Insight Integrating, Executing and Adjusting, and Feedback Recollecting five aspects. Among them, Executing and Adjusting the project is a bridge connecting Brainstorming, Researching, Insight Integrating and Feedback Recollecting. Throughout IHP, we build a cycle of finding meaning, reaching out, and connecting our projects to the world."}]},{"type":"element","tag":"h2","props":{"id":"brainstorming"},"children":[{"type":"text","value":"Brainstorming"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Our team is focusing on detecting new pollutants this year. At the beginning of the project, we carried out extensive research on new pollutants, and focused on endocrine disruptors, microplastics, antibiotics, persistent pollutants four, because of their greater harm and detection methods are expensive and difficult to operate."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Since then, endocrine disruptors have been screened according to the difficulty of detection, the harm to the environment and the significance of positive results. In the traditional evaluation system of endocrine disruptors, most of the main methods are to detect their binding with hormone receptors. However, due to the extensive and complex toxic effects of endocrine disruptors and their metabolites on organisms, the traditional detection methods are obviously inadequate."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The result of our brainstorming is to build a gate-based multi-toxicological screening sensor that can simultaneously detect endocrine disrupting effects and DNA damage effects in factory and enterprise products, or unknown samples in the environment."}]},{"type":"element","tag":"h2","props":{"id":"researching"},"children":[{"type":"text","value":"Researching"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"After determining the study of endocrine disruptors, we screened out methods that could detect endocrine disruptors through literature research and conducted feasibility analysis. Finally, a method was established to detect endocrine disruptors by constructing hormone analogues sensitive peptides and coupled with Escherichia coli SOS oxidative damage response system and hrp amplifier for detecting DNA damage effects to construct a gated multi-toxicological effects screening sensor design. In addition, we investigated the contained peptide sequence and found that the number of bases was too large, which would cause a heavy burden on Escherichia coli. Therefore, the components for detecting the two effects were divided into two bacteria, and the LuxI/LuxR system was used for communication. Biosensors use fluorescent protein reports.\nThis is the initial design of our project."}]},{"type":"element","tag":"h2","props":{"id":"insight-integrating"},"children":[{"type":"text","value":"Insight Integrating"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In order to identify the social needs of our project with stakeholders, we contacted environmental testing companies, government departments, factories and wastewater treatment plants to ask about the needs and status of pollutant monitoring. In addition, in order to enhance the professional and scientific nature of the project, we also consult with researchers in related fields to adjust our research direction and research content."}]},{"type":"element","tag":"h3","props":{"id":"government-department"},"children":[{"type":"text","value":"Government Department"}]},{"type":"element","tag":"h4","props":{"id":"bureau-of-ecology-environment-and-environmental-protection"},"children":[{"type":"text","value":"Bureau of Ecology Environment and Environmental Protection"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"On May 23, 2024, our team members approached Ms. Luo, an official of Chongqing Ecological Environment and Environmental Protection Bureau, to consult the social needs of our project.\nFirst of all, we consulted the definition method, detection and registration process and control measures of endocrine disruptors. Luo pointed out that after confirming that a substance is polluting, the government departments will commission professional technical institutions to conduct research and investigation every year, and if the result is ultra-ultra-trace, relatively safe, no further measures will be taken. But if the concentration is high, the government will take measures and test the water quality of the test site or its source to track its possible source. In the analysis of new pollutants, including endocrine disruptors, government departments will conduct toxicological tests on chemical substances based on cutting-edge international research to determine that a substance may be harmful to the human body. This is a very important part of the testing process, because the government departments will concentrate the samples, which will lead to a variety of substances will be detected, and only when these substances are associated with toxicological effects and confirmed to be harmful to the human body, it is necessary to define endocrine disruptors and monitor. In terms of control, the government has corresponding emission standards for well-known endocrine disruptors; For new pollutants such as endocrine disruptors detected, the government will continue to confirm whether they belong to the emission substances already on the control list. For the situation where the pollutant index exceeds the standard, the government department will require the enterprise to take corresponding treatment measures and implement management measures such as regular review in the follow-up to supervise the rectification results."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"As for endocrine disruptors, Luo pointed out that in some studies, all organic compounds were classified as endocrine disruptors under investigation, which is not accurate; The current approach is to study each emerging substance independently and then classify it. The test site of endocrine disruptors is generally in other important water bodies such as drinking water sources, livestock and poultry farms and chemical plants and other emission sources."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Secondly, we proposed a method to determine whether the water quality contains endocrine disruptors by using the biosensor constructed by the engineering bacteria that specifically recognizes endocrine disruptors. Luo first confirmed that in the environmental field, the emergency equipment launched abroad in the past few years does include microbial sensors and fluorescence detection technology to deal with sudden emergency environmental accidents. For endocrine disruptors, the government does not include them in routine testing tasks, but they are regularly included in testing efforts. The government prefers to use more accurate tests to detect endocrine disruptors. Therefore, from this point of view, improving the accuracy and sensitivity of the detection is also very beneficial and important."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In short, Luo affirmed our project's detection method of using highly sensitive and specific biosensors for endocrine disruptors in the environment, which is very necessary and beneficial for government departments to detect endocrine disruptors, and our project is in line with the needs of government departments."}]},{"type":"element","tag":"h3","props":{"id":"environmental-testing-company"},"children":[{"type":"text","value":"Environmental Testing Company"}]},{"type":"element","tag":"h4","props":{"id":"shanghai-environmental-monitoring-technology-co-ltd"},"children":[{"type":"text","value":"Shanghai Environmental Monitoring Technology Co., Ltd"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/ihp/guo.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In order to continue to understand the definition method and testing registration process of new pollutants, on August 1, 2024, we found Mr. Guo, chief engineer of Shanghai Environmental Monitoring Technology Co., Ltd. for consultation."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Guo pointed out that the determination of pollutants is first based on those already found by the country, and their emissions often exceed the prescribed standards, while classification and the formulation of standards are needed. The study of these pollutants needs to determine whether they have harmful effects on the human body through effect analysis, toxicology analysis and process analysis. Secondly, the use of foreign literature research combined with the actual situation in China to develop a standardized detection process to find endocrine disruptors. The definition of most new pollutants, especially endocrine disruptors, is that they are first found to be harmful to human health, and then they are analyzed in detail."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"For the analysis standards of new pollutants such as endocrine disruptors, Guo mentioned that with the development of industry, some new substances will be continuously studied, but whether it is harmful to the human body needs to be determined through a scientific judgment process, and if it is found to be harmful to the human body, it needs to be immediately included in the supervision. According to the requirements of the state, Shanghai has put the new pollutants to be studied into the supervision. Both testing organizations and industry systems are constantly developing methods to detect new pollutants, including endocrine disruptors, and current detection instruments are sophisticated and expensive."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In the subsequent improvement process, the hardware device has undergone a simplified design to achieve the above design goals of portability and miniaturization."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In the communication with Luo and Guo, they both affirmed our thinking and importance of detecting new pollutants such as endocrine pollutants through toxicological effects."}]},{"type":"element","tag":"h3","props":{"id":"factory-and-enterprise"},"children":[{"type":"text","value":"Factory and Enterprise"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Our next step is to understand the current status of the detection and analysis of endocrine disruptors from the perspective of relevant units and companies, as well as the specific needs currently existing, and ask whether there are applicable scenarios for the project. In accordance with Luo's previous guidance, we found a sewage plant and a water plant in Shanghai on August 1, 2024."}]},{"type":"element","tag":"h4","props":{"id":"shanghai-taihe-wastewater-treatment-plant"},"children":[{"type":"text","value":"Shanghai Taihe Wastewater Treatment Plant"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/ihp/liu.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We have found Mr. Liu, director of Shanghai Taihe Sewage Plant. His reply to the above question is that the main pollutants under supervision of the sewage plant are Cod, Bod, ammonia, nitrogen and phosphorus. However, the pollutants under supervision of the sewage plant are to send water samples to testing institutions for testing, and domestic sewage involves few substances with toxicological properties."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"For our project, Mr. Liu said that due to the above reasons, the emergence of relevant technologies is temporarily lacking. Therefore, we should reflect on the use cases of our own projects and focus on providing services to government departments and environmental monitoring agencies."}]},{"type":"element","tag":"h4","props":{"id":"shanghai-nanhui-water-plant"},"children":[{"type":"text","value":"Shanghai Nanhui Water Plant"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/ihp/ma.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In the drinking water industry, we found Mr. Ma, director of Shanghai Nanhui Water Plant, for consultation. In response to the above problems, the director pointed out that the detection of new pollutants in the drinking water industry, including endocrine disruptors, must use more expensive instruments, and the detection requirements and operations are more complicated; There is a need for emergency detection of source water for the use of biosensors. In view of our team's project, the factory director put forward that it can be applied in the rapid emergency monitoring of the above source water, and put forward the suggestions and technical requirements of our products for both qualitative and quantitative testing of drinking water source water."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Therefore, for factories and enterprises, our project has a demand in the rapid emergency monitoring of drinking water source water, and we need to design a gate-based multi-toxicological effect screening sensor that can carry out qualitative and quantitative analysis on the basis of the project and is equipped with portable detection equipment."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In short, the need for endocrine disruptor testing that we investigated is actually confirmed. At present, the detection of new pollutants including endocrine disruptors is more passive, and the method is more heavy on toxicological analysis, and has high cost and cumbersome operation. Therefore, there is a real need for a highly sensitive and specific detection of endocrine disruption and DNA damage in unknown samples, two important toxicological effects, and the hardware of such sensors must be portable and miniaturized."},{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/ihp/projectpropose.png"},"children":[]}]},{"type":"element","tag":"h3","props":{"id":"professional-research-personnel"},"children":[{"type":"text","value":"Professional research personnel"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/ihp/yang.jpg"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We had an in-depth discussion on Modeling with Professor Yang Guanghui of China Agricultural University. He first gave a positive opinion of our idea. As the latest achievement in the field of bioinformatics, Alphafold3 has a high degree of confidence in the prediction of protein structure function, which can effectively guide our research in synthetic biology. But he also points out that the number and length of hydrogen bonds alone cannot accurately measure how folded a protein domain is. The main force in protein folding is hydrophobic interaction. However, there are no effective means to quantitatively measure the degree of protein folding and the strength of hydrophobic interaction. He encouraged us to work in this direction, hoping that we would be able to measure protein folding from other angles."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Inspired by this, and after subsequent investigation, we will use Chimera1.13 software to analyze the hydrophobic interaction of the protein conformation obtained by molecular dynamics simulation (MDS), and measure the hydrophobic interaction intensity by comparing the hydrophobic potential around the recAN end and the C-end, so as to compare the degree of folding of this domain in the protein"}]},{"type":"element","tag":"h2","props":{"id":"feedback-recollecting"},"children":[{"type":"text","value":"Feedback Recollecting"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We plan to bring the prototype design to endocrine disruptor experts and previous stakeholders, and continue to receive their comments and feedback to continue to adapt our project; At the same time, we will also find new innovation points and needs from the feedback, so as to launch a new round of \"BRIEF\" cycle in the future and complete the closed loop."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/ihp/executingandadjusting.png"},"children":[]}]}],"toc":{"title":"","searchDepth":1,"depth":3,"links":[{"id":"overview","depth":2,"text":"Overview"},{"id":"brainstorming","depth":2,"text":"Brainstorming"},{"id":"researching","depth":2,"text":"Researching"},{"id":"insight-integrating","depth":2,"text":"Insight Integrating","children":[{"id":"government-department","depth":3,"text":"Government Department","children":[{"id":"bureau-of-ecology-environment-and-environmental-protection","depth":4,"text":"Bureau of Ecology Environment and Environmental Protection"}]},{"id":"environmental-testing-company","depth":3,"text":"Environmental Testing 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(Mtu)的内含肽RecA。我们想将其放入我们的工程菌中，其中我们需要稳定的RecA intein并将其插入T7 RNAP中，以检测内分泌干扰效应。这就提出了一个有趣的问题，即“我们将RecA插入在哪里才能得到最高效稳定的T7RNAP-intein？“这为使用 Dry Lab 来扩展我们在 Wet Lab 中所做的工作提供了机会，因为鉴于没有评价蛋白质效果的统一方法，我们决定模拟 T7 RNAP的每种可能的插入位点所形成的蛋白质，以及从与雌二醇的结合效果、静电势分析、能量分析等角度综合评估选择最优蛋白质"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"最优蛋白质结构分析"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"1.蛋白质结构预测"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"查阅资料得知，RecA可能的插入位点集中在半胱氨酸旁，于是根据T7 RNAP的肽链得到十二种可能的工程蛋白T7RNAP-intein。并使用alphafold3来进行结构预测。同时作为参照采取……的同源建模方式加以佐证"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"2.雌激素结合效果"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"3.静电势分析"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"pymol的APBS插件能够分析并计算电势分布，而电势分布较为均匀的蛋白质"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"4.能量分析"}]},{"type":"element","tag":"h3","props":{"id":"微控流芯片建模"},"children":[{"type":"text","value":"微控流芯片建模"}]},{"type":"element","tag":"h3","props":{"id":"reference"},"children":[{"type":"text","value":"reference"}]}],"toc":{"title":"","searchDepth":1,"depth":3,"links":[{"id":"models","depth":2,"text":"Models","children":[{"id":"overview","depth":3,"text":"Overview","children":[{"id":"基因层模型","depth":4,"text":"基因层模型："}]},{"id":"蛋白质模型","depth":3,"text":"蛋白质模型："},{"id":"微控流芯片建模","depth":3,"text":"微控流芯片建模"},{"id":"reference","depth":3,"text":"reference"}]}]}},"_type":"markdown","_id":"content:models.md","_source":"content","_file":"models.md","_stem":"models","_extension":"md"},{"_path":"/project-description","_dir":"","_draft":false,"_partial":false,"_locale":"","title":"Description","description":"","body":{"type":"root","children":[{"type":"element","tag":"h2","props":{"id":"background"},"children":[{"type":"text","value":"Background"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Environmental endocrine disruptors (EDCs) are a class of chemical substances that can mimic or block the effects of hormones, affecting the normal development of organisms. These substances include DDT, dioxins, and phthalates, among others."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"..."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"DDT is an insecticide that was once widely used, but due to its harmful effects on the reproductive and nervous systems of organisms, as well as its potential carcinogenicity, its use has been restricted in many countries since the 1970s and 1980s. However, as these substances have already entered the environment in large quantities, their negative effects persist even though their production has stopped for many years. They accumulate in the atmosphere, soil, water bodies, and organisms, posing a natural hazard."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In 2010, the direct social and economic loss caused by exposure to phthalate chemicals in China reached approximately 57.2 billion yuan, with the burden of male infertility being the most severe. Further calculations indicate that the total medical cost for all known EDCs exposure in China is about 429.4 billion yuan, accounting for approximately 1.07% of the annual GDP."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"..."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"All these indicate that we must pay attention to these potential dangers and have a more comprehensive understanding and early screening of environmental endocrine disruptors. Nowadays, with the deepening research on environmental endocrine disruptors, more and more studies are beginning to focus on the relationship between endocrine disruptors and their metabolites and other toxic effects. This superposition of multiple effects provides clues to revealing the molecular mechanisms underlying many diseases."}]},{"type":"element","tag":"h2","props":{"id":"problem"},"children":[{"type":"text","value":"Problem"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In fact, the role of environmental endocrine disruptors in organisms is complex, and the inference of their adverse effects needs to emphasize the adverse outcome pathway (AOP pathway, which describes the logical derivation of causally related events at different levels of biological tissues, leading to adverse effects on human or wildlife health). This requires us to describe the molecular mechanisms behind the impact comprehensively."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Currently, the methods for detecting endocrine disruptors using biological effects mainly include competitive ligand binding assays, fused cell aggregation experiments, and animal cell proliferation analysis experiments. These methods can only evaluate the endocrine disruption effect through the binding effect of hormone receptors when analyzing endocrine disruptors, and cannot judge the involvement of other effects behind the biological damage caused by endocrine disruptors, therefore, they have limitations."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"More and more literature has proven the important role of oxidative damage effects in the damage caused by environmental endocrine disruptors to organisms. For example, exposure to various parabens (PBs) and their metabolites can increase the level of oxidative stress, and the superposition of oxidative damage and endocrine disruption increases the risk of impaired reproductive capacity in organisms. The collaborative analysis of environmental endocrine disruption effects and oxidative damage effects can help us predict the pathways of action of environmental endocrine disruptors on organism damage, thereby assisting us in having a clearer understanding of their damage consequences and providing a reference for regulatory authorities to implement targeted regulatory policies."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Our project is based on this concept and aims to construct microbial sensors through synthetic biology methods to provide multiple detection lines for the assessment of endocrine disruption effects and oxidative damage effects. Not only can we achieve the separate detection of the two effects, but we can also realize the collaborative analysis of the two effects through logic gate detection lines, achieving a rapid, convenient, and comprehensive assessment of environmental endocrine disruptors. Conduct more in-depth research and discussion on the impact mechanism of environmental endocrine disruptors."}]},{"type":"element","tag":"h2","props":{"id":"inspiration"},"children":[{"type":"text","value":"Inspiration"}]},{"type":"element","tag":"h3","props":{"id":"biology"},"children":[{"type":"text","value":"biology"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Initially, we planned to develop a biosensor for detecting environmental endocrine disruptors. To achieve this goal, we studied the characteristics and mechanisms of action of environmental endocrine disruptors and researched the methods for detecting them. Intein peptides are protein analogs found in self-splicing RNA introns, and their insertion can lead to the inactivation of the host protein. Typically, they excise themselves from the host cell after translation, restoring the activity of the host protein. By replacing the homing endonuclease domain in the intein peptide with a hormone receptor binding domain, we can construct a hormone-sensitive intein peptide, which can be used to create recognition elements for endocrine disruption effects. We plan to construct an estrogen-sensitive intein peptide and insert it into T7 RNA Polymerase. T7 RNA Polymerase can specifically activate the T7 promoter, thus regulating the downstream circuit to express the reporter gene upon detection of estrogen mimics through the T7 promoter."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Subsequently, through more in-depth researches, it was found that the effects of environmental endocrine disruptors in organisms are complex. An increasing number of studies have demonstrated the significant role of oxidative stress in the damage caused by environmental endocrine disruptors. For example, exposure to various paraben compounds (PBs) and their metabolites can increase oxidative stress levels. The combined oxidative damage and endocrine disruption can heighten the risk of impaired reproductive capability in organisms. A synergistic analysis of the effects of environmental endocrine disruption and oxidative damage can help us predict the pathways through which these disruptors cause harm to organisms, thereby assisting us in gaining a clearer understanding of their damaging consequences. Therefore, we plan to incorporate DNA damage detection, designing an and gate detection circuit capable of simultaneously detecting endocrine disruption effects and DNA oxidative damage effects."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"DNA damage detection is based on the SOS response mechanism in "},{"type":"element","tag":"em","props":{},"children":[{"type":"text","value":"Escherichia coli"}]},{"type":"text","value":", which is an inducible DNA repair system activated when bacterial DNA is damaged. When bacteria detect DNA damage, the expression levels of genes associated with the SOS response mechanism significantly increase, leading to the expression of proteases that relieve the repression of target proteins, initiating the repair process. We chose the recA promoter to detect DNA damage, when bacteria sense DNA damage, the expression level of this promoter rises significantly, thereby activating downstream reporter genes."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To construct the logic gate, we introduced the hrp system, which consists of the hrpR and hrpS genes along with the HrpL promoter regulated by the sigma54 factor. The hrpL promoter is activated only when both hrpR and hrpS genes are expressed simultaneously."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We plan to insert the hrpR gene downstream of the P recA promoter, which will be activated in response to DNA damage effects; the hrpS gene will be inserted downstream of the P T7 promoter, which will be activated after the peptide-specific binding domain binds to estrogen analogs. The reporter gene egfp will be inserted downstream of the P hrpL promoter, resulting in a two-input AND gate circuit."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"However, the entire circuit is too large, posing significant challenges to the stability of the plasmid and the burden on the engineered bacteria. Therefore, we plan to divide the entire circuit into two parts, each constructed in different engineered bacteria, inserting new components to enable signal transmission between the two engineered bacterias."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Throughout the system, we have always planned to use the egfp gene as the reporter gene. However, this gene requires the presence of oxygen molecules as a cofactor for its luminescence, while the cultivation chamber of our designed hardware is in a low-oxygen environment. As a result, we tested a new reporter gene, BsFbFp, which can express significantly enhanced in vivo fluorescence in both aerobic and anaerobic environments, ensuring that the engineered bacteria can effectively express fluorescent signals in low-oxygen conditions."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Thus, the construction of the biological part of our circuit has been completed."}]},{"type":"element","tag":"h3","props":{"id":"hardware"},"children":[{"type":"text","value":"Hardware"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Point of Care Testing (POCT) is a form of rapid on-site testing/near-patient testing method which has been particularly impactful and is playing an increasingly vital role in the fields of medical diagnostics and environmental health monitoring due to its speed, convenience, and ease of operation. Traditional water contaminant detection technologies are often constrained by the need for costly equipment and a professional laboratory environment. In contrast, novel biosensor detection devices based on compact POCT equipment, when connected to an interactive app for operational control and visual data analysis, can effectively facilitate the rapid and efficient screening of emerging waterborne pollutants. This approach holds broad prospects for application in the field of environmental monitoring and assessment."}]},{"type":"element","tag":"h2","props":{"id":"solution"},"children":[{"type":"text","value":"Solution"}]},{"type":"element","tag":"h3","props":{"id":"biology-1"},"children":[{"type":"text","value":"biology"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To achieve signal communication between the two engineered bacteria, we studied the bacterial quorum sensing mechanism. This mechanism allows bacteria to exchange information and coordinate population behaviors when their numbers reach a certain density, regulating gene expression. When activated, bacteria secrete signal molecules that can freely diffuse across the cell membrane. Once the concentration of these signal molecules in the environment reaches a certain threshold, it induces the expression of specific genes in the bacteria. We introduced the LuxI/R system, where AHL (N-acyl homoserine lactone) is produced catalytically by the expression of the LuxI gene as a signaling molecule. This AHL freely diffuses into the second engineered bacteria and binds with the LuxR protein released by the activation of the LuxR gene, activating the P LuxR promoter to initiate downstream gene expression."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/description/biocircuit-p1.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/description/biocircuit-p2.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/description/biocircuit-p3.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/description/biocircuit-p4.png"},"children":[]}]},{"type":"element","tag":"h3","props":{"id":"hardware-1"},"children":[{"type":"text","value":"Hardware"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In response to the demands for standardized cultivation of engineered bacteria and high-sensitivity detection, we have designed a highly integrated and automated system that encompasses the entire process of bacterial strain revival, cultivation, detection, and analysis. The system utilizes an ESP32 chip as the main controller, interfaces with a temperature control system and a fluorescence detection system, employs a linear slide rail structure for high-throughput detection, and leverages a mobile app for automated device control and visualization of detection results. This system overcomes the limitations of time and space associated with traditional detection equipment, providing a versatile, portable, and highly sensitive solution for the detection of EDCs in aquatic environments across various scenarios."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/description/and-gate.png"},"children":[]}]},{"type":"element","tag":"h2","props":{"id":"reference"},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"text","value":"Reference"}]}]},{"type":"element","tag":"mdc-wrap","props":{":class":"reference"},"children":[{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Cao Y, Li L, Shen KH, Liu JG. Disease burden attributable to endocrine-disrupting chemicals exposure in China: A case study of phthalates. Science of the Total Environment, 2019, Vol. 662(1): 615-621"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Nematollah Asadi, Mahmoud Bahmani, Arash Kheradmand, Mahmoud Rafieian-Kopaei. The Impact of Oxidative Stress on Testicular Function and the Role of Antioxidants in Improving it: A Review. Journal of Clinical and Diagnostic Research, 2017, Vol. 11(5): IE1-IE5"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Ma Ya, Liu Haohao, Wu Jinxia, Yuan Le, Wang Yueqin, Du Xingde, Wang Rui, Marwa Phelisters Wegesa, Petlulu Pavankumar, Chen Xinghai, Zhang Huizhen. The adverse health effects of bisphenol A and related toxicity mechanisms. Environmental Research, 2019, Vol. 176: 108"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Liang R , Zhou J , Liu J. Construction of a Bacterial Assay for Estrogen Detection Based on an Estrogen-Sensitive Intein. Applied & Environmental Microbiology, 2011, 77(7):2488.DOI:10.1128/AEM.02336-10."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Ma Ya, Liu Haohao, Wu Jinxia, Yuan Le, Wang Yueqin, Du Xingde, Wang Rui, Marwa, Phelisters Wegesa, Petlulu, Pavankumar, Chen Xinghai, Zhang Huizhen.The adverse health effects of bisphenol A and related toxicity mechanisms.Environmental Research, 2019, Vol.176: 108575"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"LIANG Jing-jia, GU Ai-hua. Multigenerational and cross-generational effect of environmental endocrine disruptors on reproductive system in male animals. "},{"type":"element","tag":"em","props":{},"children":[{"type":"text","value":"Chinese Journal of Public Health"}]},{"type":"text","value":", 2021, 37(2): 375-380."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Nematollah Asadi, Mahmoud Bahmani, Arash Kheradmand, Mahmoud Rafieian-Kopaei.The Impact of Oxidative Stress on Testicular Function and the Role of Antioxidants in Improving it: A Review.Journal of Clinical and Diagnostic Research, 2017, Vol.11(5): IE1-IE5"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Mondal Shirsha, Ghosh Songita, Bhattacharya Samir, Mukherjee Sutapa. Chronic dietary administration of lower levels of diethyl phthalate induces murine testicular germ cell inflammation and sperm pathologies: Involvement of oxidative stress. Chemosphere, 2019, Vol.229: 443-451"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Maslowska K H, Karolina Makiela‐Dzbenska, Fijalkowska I J. The SOS system: A complex and tightly regulated response to DNA damage. John Wiley & Sons, Ltd, 2019(4). DOI:10.1002/em.22267."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Baojun W, Mauricio B, Martin B. Engineering modular and tunable genetic amplifiers for scaling transcriptional signals in cascaded gene networks. Nucleic Acids Research, 2014(14): 9484-9492. DOI:10.1093/nar/gku593."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Xiao Y, Zou H, Li J, Song T, Lv W, Wang W, Wang Z, Tao S. Impact of quorum sensing signaling molecules in gram-negative bacteria on host cells: current understanding and future perspectives. Gut Microbes. 2022 Jan-Dec, 14(1):2039048. doi: 10.1080/19490976.2022.2039048."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Drepper T, Eggert T, Circolone F, et al. Reporter proteins for in vivo fluorescence without oxygen. Nature Biotechnology, 2007, 25(4):443-445.DOI:10.1038/nbt1293."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Mukherjee A, Schroeder C M, Sikes H D, et al. Flavin-based fluorescent proteins: emerging paradigms in biological imaging. "},{"type":"element","tag":"em","props":{},"children":[{"type":"text","value":"This review comes from a themed issue on Analytical biotechnology"}]},{"type":"text","value":". 2015."}]}]}]}],"toc":{"title":"","searchDepth":1,"depth":3,"links":[{"id":"background","depth":2,"text":"Background"},{"id":"problem","depth":2,"text":"Problem"},{"id":"inspiration","depth":2,"text":"Inspiration","children":[{"id":"biology","depth":3,"text":"biology"},{"id":"hardware","depth":3,"text":"Hardware"}]},{"id":"solution","depth":2,"text":"Solution","children":[{"id":"biology-1","depth":3,"text":"biology"},{"id":"hardware-1","depth":3,"text":"Hardware"}]},{"id":"reference","depth":2,"text":"Reference"}]}},"_type":"markdown","_id":"content:project-description.md","_source":"content","_file":"project-description.md","_stem":"project-description","_extension":"md"},{"_path":"/project-engineering","_dir":"","_draft":false,"_partial":false,"_locale":"","title":"Project Engineering","description":"","body":{"type":"root","children":[{"type":"element","tag":"h2","props":{"id":"biosensor"},"children":[{"type":"text","value":"Biosensor"}]},{"type":"element","tag":"h3","props":{"id":"luminescence"},"children":[{"type":"text","value":"Luminescence"}]},{"type":"element","tag":"h4","props":{"id":"circle-design-reca-bsfbfp"},"children":[{"type":"text","value":"Circle: Design recA-BsFbFp"}]},{"type":"element","tag":"h5","props":{"id":"design"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In order to adapt to the low oxygen environment of the hardware design, we replaced the egfp gene, which strictly requires oxygen molecules as a cofactor for its luminescence, with the reporter gene BsFbFp. This gene can effectively express fluorescent signals in low or anaerobic environments."}]},{"type":"element","tag":"h5","props":{"id":"build"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"BsFbFp circuits","src":"https://static.igem.wiki/teams/5358/engineering-success/bsfbfp-circuits.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 1. BsFbFp circuits"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\tGenetic circuit: Gene recA and BsFbFp were all synthesized by Sangon Biotech according to BIOBRICK standard, and the gene circuit was obtained by PCR and verified by agarose gel electrophoresis."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"build results of BsFbFp ","src":"https://static.igem.wiki/teams/5358/engineering-success/build-results-of-bsfbfp.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 2. Build results of BsFbfP"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Construction of plasmid: The results of the agarose gel electrophoresis successfully showed bands for recA, the vector and BsFbFp, confirming that the gene circuit was successfully connected to the vector."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Ideal Strain: The successfully constructed plasmid was transformed into BL21 competent cells and cultured on LB solid medium with ampicillin."}]},{"type":"element","tag":"h5","props":{"id":"test"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Adding 600μL of bacterial solution and 30μL of ampicillin to 30mL of fresh culture medium, logarithmic growth was performed. Then, NA (Nalidixic Acid) was added in a gradient based on concentration to induce the bacteria. Subsequently, 250μL from each group was added to a anaerobic chamber and incubated on a shaking incubator at 37°C with a speed of 180 r/min. After 2 hours, the OD value and fluorescence intensity were measured."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"RFU-BsFbFp in 10lianpaichip(anaerobic chamber)","src":"https://static.igem.wiki/teams/5358/engineering-success/rfu-bsfbfp-in-10lianpaichip-anaerobic-chamber.jpg"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 3. RFU-BsFbFp in 10lianpaichip(anaerobic chamber)"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​The RFU (Relative Fluorescence Units) of the three groups of engineered bacteria increased linearly with the amount of NA (Nalidixic Acid) added. The results indicate that the fluorescent expression circuit of BsFbFp has been successfully constructed, and the engineered bacteria can effectively express fluorescence in an anaerobic environment."}]},{"type":"element","tag":"h5","props":{"id":"learn"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In conclusion，the plasmid constructed using BsFbFp as a reporter gene can effectively express fluorescence in low-oxygen or anaerobic environments, which is beneficial for the design of the hardware components."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​So we believed that the work in this cycle has been successfully accomplished., and we obtained the engineered bacteria needed for our goals."}]},{"type":"element","tag":"h3","props":{"id":"and-gate-design"},"children":[{"type":"text","value":"AND Gate Design"}]},{"type":"element","tag":"h4","props":{"id":"circle-1-design-pet28-reca-hrp-egfp"},"children":[{"type":"text","value":"Circle 1: Design pET28-recA-HRP-egfp"}]},{"type":"element","tag":"h5","props":{"id":"design-1"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​To construct the and gate, we introduced the hrp system, composed of the hrpR gene and hrpS gene, as well as the HrpL promoter regulated by the sigma54 factor. The hrpL promoter will only be activated when both the hrpR gene and the hrpS gene are expressed simultaneously."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In 2023, the BIT team successfully constructed P recA, the Hrp system, and egfp on the pUC19 plasmid. However, tests showed that the expression level of the circuit constructed using the pUC19 plasmid as a vector was relatively low, significantly deviating from the expected results. Therefore, we plan to switch to the pET28 plasmid, which has a more prominent expression level, to facilitate the synergistic assessment of endocrine disruption effects and oxidative damage effects."}]},{"type":"element","tag":"h5","props":{"id":"build-1"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Genetic circuit: recA-HRP-egfp were synthesized by Sangon Biotech according to BIOBRICK standard， the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir1-1","src":"https://static.igem.wiki/teams/5358/engineering-success/cir1-1.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 4. enzyme digestion result of circle 1"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nThe results of the agarose gel electrophoresis indicated that the vector was successfully digested and the target fragment was successfully amplified by PCR. The ligation was carried out at a 1:4 ratio of vector to target fragment, followed by transformation into DH5α competent cells and verification through agarose gel electrophoresis."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir1-2","src":"https://static.igem.wiki/teams/5358/engineering-success/cir1-2.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 5. build result of circle 1"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nVerification results: In multiple rounds of construction validation experiments, the target fragment showed no band in the agarose gel electrophoresis results, and the restriction enzyme digestion verification indicated self-ligation of the vector, proving that the construction in this cycle has failed."}]},{"type":"element","tag":"h5","props":{"id":"test-1"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Due to the failure of the target plasmid construction in this cycle, it is necessary to analyze the issues present in the plasmid design and construction process and make corrections."}]},{"type":"element","tag":"h5","props":{"id":"learn-1"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​During this construction process, we overlooked the reagent concentration ratio of the vector to the target fragment in our ligation system, which may be a major factor contributing to the failure of ligation. We plan to introduce the concentration factor of the ligation reagents into the ratio to improve the efficiency of ligation."}]},{"type":"element","tag":"h4","props":{"id":"circle-2-adjust-the-ligation-system-and-reconstruct-the-plasmid-pet28-reca-hrp-egfp"},"children":[{"type":"text","value":"Circle 2: Adjust the ligation system and reconstruct the plasmid pET28-recA-HRP-egfp."}]},{"type":"element","tag":"h5","props":{"id":"design-2"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Based on the experience learned from Circle 1, we plan to use a new ligation system, increase the dosage of the target fragment, and improve the ligation efficiency."}]},{"type":"element","tag":"h5","props":{"id":"build-2"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​The construction process used three different ligation systems, with the ratios of vector to target fragment being 1:4, 1:5, and 1:6, respectively. The constructed plasmids were extracted and verified by PCR followed by agarose gel electrophoresis."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir2","src":"https://static.igem.wiki/teams/5358/engineering-success/cir2.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 6. built result of circle 2"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nConstruction of plasmid: In the multi-round adjustment experiments of the ligation system construction, the agarose gel electrophoresis results still did not show a band for the target fragment, indicating that the construction in this cycle was unsuccessful."}]},{"type":"element","tag":"h5","props":{"id":"test-2"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Due to the failure of target plasmid construction in this cycle, testing cannot be carried out."}]},{"type":"element","tag":"h5","props":{"id":"learn-2"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In this cycle, we have corrected the ligation system, removing this influencing factor. Therefore, we suspect that the low partial digestion efficiency at the target sequence enzyme cutting sites may be the cause. This could be due to issues with the setting of protective bases near the enzyme cutting sites when designing the PCR primers, leading to another ligation failure. As such, we plan to redesign the PCR primers and increase the number of protective bases on both sides of the enzyme cutting sites from one or none to three to improve the success rate of the ligation."}]},{"type":"element","tag":"h4","props":{"id":"circle-3-redesign-the-primers-increase-protective-bases-and-reconstruct-pet28-reca-hrp-egfp"},"children":[{"type":"text","value":"Circle 3: Redesign the primers, increase protective bases, and reconstruct pET28-recA-HRP-egfp"}]},{"type":"element","tag":"h5","props":{"id":"design-3"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Based on the experience learned from Circle 2, we have redesigned the PCR primers for the target fragment and added protective bases to them, and we are reconstructing the pET28-recA-HRP-egfp plasmid."}]},{"type":"element","tag":"h5","props":{"id":"build-3"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​After changing to new PCR primers and setting the protective bases on both sides of the restriction enzyme cutting sites to 3, the construction was carried out. Genetic circuits: recA-HRP-egfp were synthesized by Sangon Biotech according the BIOBRICK standard, the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir3","src":"https://static.igem.wiki/teams/5358/engineering-success/cir3.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 7. built result of circle 3"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nThe agarose gel electrophoresis results showed that after redesigning the PCR primers and increasing the number of protective bases on both sides of the restriction enzyme cutting sites from one or none to three, only one band was observed for the target fragment, indicating that the new PCR primers have strong specificity and the target fragment was successfully obtained.\n​\tHowever, due to time constraints, we did not proceed with the subsequent experiments."}]},{"type":"element","tag":"h5","props":{"id":"test-3"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Limited by time, we were unable to complete the reconstruction, and therefore have no test results."}]},{"type":"element","tag":"h5","props":{"id":"learn-3"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In this cycle, the new PCR primers and the addition of protective bases at both ends of the restriction enzyme sites are beneficial for improving the success rate of enzyme ligation, thereby providing an experimental basis for the successful construction of the gate circuit."}]},{"type":"element","tag":"h2","props":{"id":"hardware"},"children":[{"type":"text","value":"Hardware"}]},{"type":"element","tag":"h4","props":{"id":"objectives"},"children":[{"type":"text","value":"Objectives"}]},{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To provide a high-throughput portable reaction vessel."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To provide stable physical conditions for detection."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"High-sensitivity fluorescence detection."}]}]},{"type":"element","tag":"h4","props":{"id":"design-4"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Our entire device mainly consists of three major parts: the outer casing that encases the entire device, the reaction area located in the upper layer, and the detection area situated in the lower layer. In the initial design, we utilized an STM32 chip as the main controller to regulate temperature control, fluorescence detection, and Bluetooth communication functions. In the second iteration, we replaced the main controller with an ESP32 chip that integrates a wireless communication module, and we adopted integrated temperature control and detection modules, which further enhances the system's stability and accuracy. To meet the need for simultaneous detection of multiple samples, we also redesigned the high-throughput reaction chambers, achieving rapid successive detection through a linear slide rail system."}]},{"type":"element","tag":"table","props":{},"children":[{"type":"element","tag":"thead","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"th","props":{},"children":[]},{"type":"element","tag":"th","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Initial Scheme"}]}]}]},{"type":"element","tag":"th","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Final Scheme"}]}]}]},{"type":"element","tag":"th","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Advantages After Improvement"}]}]}]}]}]},{"type":"element","tag":"tbody","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"MCU"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"STM32F103C8T6"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Arduino Nano ESP32"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Integrate Bluetooth communication module, reduce the complexity of the design"}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Temperature Control"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"TB6612FNG"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"TCM1040"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"More precise control of temperature"}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Fluorescence Detection"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"AS7341"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"FM-4A"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Integrate excitation light and fluorescent sensor to make the detection result more stable."}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Reaction Platform"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Single Reaction Tube"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Slide Rail Platform"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Achieve high-throughput detection"}]}]}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["tab-title"]},"children":[{"type":"text","value":"Table 1. Scheme difference"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nIn terms of material selection, we have adopted a black silicone kit and an aluminum alloy temperature-controlled slot as the environment for the centrifuge tubes. This scheme can excellently meet the insulation requirements of the design."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Edition Scheme Process","src":"https://static.igem.wiki/teams/5358/engineering-success/edition-scheme-process.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 8. Edition Scheme Process"}]}]},{"type":"element","tag":"h4","props":{"id":"establishment"},"children":[{"type":"text","value":"Establishment"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\tThe system construction utilized common integrated modules, with the main controller being an ESP32 chip, and temperature control and detection were accomplished using the TCM temperature control module and LIDE Technology's FM series digital fluorescence detection module, respectively. We employed industrial processing techniques to fabricate the aluminum alloy temperature-controlled slots, while the casing and other components were created using 3D printing technology."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Schematic Diagram of Hardware Equipment","src":"https://static.igem.wiki/teams/5358/engineering-success/new.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 9. Schematic Diagram of Hardware Equipment"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Model Diagram of Hardware Equipment","src":"https://static.igem.wiki/teams/5358/engineering-success/model-diagram-of-hardware-equipment.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 10. Model Diagram of Hardware Equipment"}]}]},{"type":"element","tag":"h4","props":{"id":"test-4"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\tAfter finalizing the initial plan, we first carried out feasibility tests to verify the functionality of the fluorescence system and the temperature control system. After assembling the initial version of the model, numerous issues were identified through usage testing and preliminary risk analysis, such as the susceptibility of the fluorescence system to interference and the instability of the temperature control system. Through a cycle of testing, feedback, improvement, and retesting, the final version of the model demonstrated excellent performance across all aspects. Experiments showed that fluorescence identification was highly accurate, and the overall aesthetics were significantly enhanced."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Results of Temperature Control Experiment","src":"https://static.igem.wiki/teams/5358/engineering-success/results-of-temperature-control-experiment.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 11. Results of Temperature Control Experiment"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Results of Flurescence Verification Experiment","src":"https://static.igem.wiki/teams/5358/engineering-success/results-of-flurescence-verification-experiment.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 12. Results of Flurescence Verification Experiment"}]}]},{"type":"element","tag":"h4","props":{"id":"learn-4"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\tDuring the process of designing the device, we encountered various challenges, such as overly complex control systems, insufficient stability, inability to conduct multi-sample testing, and issues with the optical path design. Throughout the real-time testing and discussion phases, we continuously documented various issues, proposed improvement plans, and actively sought help from knowledgeable teachers and consultants. Eventually, we completed a hardware design that met the requirements. Through continuous learning, improvement, and questioning, our expertise in the relevant fields has been greatly boosted."}]},{"type":"element","tag":"h2","props":{"id":"software"},"children":[{"type":"text","value":"Software"}]},{"type":"element","tag":"h4","props":{"id":"objectives-1"},"children":[{"type":"text","value":"Objectives"}]},{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To implement human-machine interaction for information exchange with hardware."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To automatically process data sent by the hardware."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To visualize data."}]}]},{"type":"element","tag":"h4","props":{"id":"design-5"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​The main body is divided into two 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discoveryAgent.finished TO this.onDeviceConnected\n​\n// Start searching for Bluetooth devices\nCALL discoveryAgent.start()\n​\n// Initialize Bluetooth Socket\nCREATE socket AS new BluetoothSocket USING RfcommProtocol\n​\n// Connect button click signal to send STARTSTART command\nCONNECT ui.pushButton.clicked TO this.sendStartSignal\n"}]}]},{"type":"element","tag":"h4","props":{"id":"plotting-module-performs-data-visualization"},"children":[{"type":"text","value":"Plotting module performs data visualization"}]},{"type":"element","tag":"pre","props":{"code":"FUNCTION updateGraphs()\n  // Create canvas\n  CREATE new scene\n  SET scene to graphicsView\n​\n  // Draw temperature graph\n  FOR i FROM 1 TO size of temperatureData - 1 DO\n    ADD line FROM (i-1, temperatureData[i-1]) TO (i, temperatureData[i]) WITH color red\n​\n  // Draw fluorescence intensity graph\n  FOR i FROM 1 TO size of fluorescenceData - 1 DO\n    ADD line FROM (i-1, fluorescenceData[i-1]) TO (i, fluorescenceData[i]) WITH color blue\n​\n  UPDATE graphicsView\nEND FUNCTION\n"},"children":[{"type":"element","tag":"code","props":{"__ignoreMap":""},"children":[{"type":"text","value":"FUNCTION updateGraphs()\n  // Create canvas\n  CREATE new scene\n  SET scene to graphicsView\n​\n  // Draw temperature graph\n  FOR i FROM 1 TO size of temperatureData - 1 DO\n    ADD line FROM (i-1, temperatureData[i-1]) TO (i, temperatureData[i]) WITH color red\n​\n  // Draw fluorescence intensity graph\n  FOR i FROM 1 TO size of fluorescenceData - 1 DO\n    ADD line FROM (i-1, fluorescenceData[i-1]) TO (i, fluorescenceData[i]) WITH color blue\n​\n  UPDATE graphicsView\nEND FUNCTION\n"}]}]},{"type":"element","tag":"h4","props":{"id":"data-segmentation-module"},"children":[{"type":"text","value":"Data segmentation module"}]},{"type":"element","tag":"pre","props":{"code":"FUNCTION splitAndFindMax(dataPoints, zeroThresholdCount)\n    INITIALIZE maxValues AS empty list\n    INITIALIZE currentSegment AS empty list\n    SET 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pET28-recA-HRP-egfp."},{"id":"circle-3-redesign-the-primers-increase-protective-bases-and-reconstruct-pet28-reca-hrp-egfp","depth":4,"text":"Circle 3: Redesign the primers, increase protective bases, and reconstruct pET28-recA-HRP-egfp"}]}]},{"id":"hardware","depth":2,"text":"Hardware","children":[{"id":"objectives","depth":4,"text":"Objectives"},{"id":"design-4","depth":4,"text":"Design"},{"id":"establishment","depth":4,"text":"Establishment"},{"id":"test-4","depth":4,"text":"Test"},{"id":"learn-4","depth":4,"text":"Learn"}]},{"id":"software","depth":2,"text":"Software","children":[{"id":"objectives-1","depth":4,"text":"Objectives"},{"id":"design-5","depth":4,"text":"Design"},{"id":"bluetooth-module-receives-data","depth":4,"text":"Bluetooth module receives data"},{"id":"plotting-module-performs-data-visualization","depth":4,"text":"Plotting module performs data visualization"},{"id":"data-segmentation-module","depth":4,"text":"Data segmentation module"},{"id":"testing","depth":4,"text":"Testing"}]}]}},"_type":"markdown","_id":"content:project-engineering.md","_source":"content","_file":"project-engineering.md","_stem":"project-engineering","_extension":"md"},{"_path":"/project-safety","_dir":"","_draft":false,"_partial":false,"_locale":"","title":"Safety","description":"Synthetic biology has the ability to change the world in amazing ways. From treating cancer to alleviating the climate crisis, we do have the ability to build a better world at iGEM. However, by working with some unpredictable organisms, we must also understand that the good works we create can actually cause harm. When looking at the big picture, we must also understand that we must be carefully every day. As researchers, we need to manage the community and environmental risks.","body":{"type":"root","children":[{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Synthetic biology has the ability to change the world in amazing ways. From treating cancer to alleviating the climate crisis, we do have the ability to build a better world at iGEM. However, by working with some unpredictable organisms, we must also understand that the good works we create can actually cause harm. When looking at the big picture, we must also understand that we must be carefully every day. As researchers, we need to manage the community and environmental risks."}]},{"type":"element","tag":"h2","props":{"id":"the-rules-and-policies-of-the-igem"},"children":[{"type":"text","value":"The rules and policies of the iGEM"}]},{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Release Beyond Containment: You cannot release or deploy any genetically modified organisms outside the lab. You must Check In before bringing any product of synthetic biology outside of the lab."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"White List: You must Check In before beginning experiments with any organism, part, or activity that is not on the White List."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"No Human Experimentation: Testing your project on humans (including yourselves) is strictly prohibited. You must check in before beginning any laboratory experiments involving humans or human samples (including but not limited to blood, DNA, other bodily specimens, and health or psychological outcomes)."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Animal Use: You must Check In and submit an Animal Use Form before beginning any experiments involving animals or animal samples. This includes vertebrates (e.g. rats, mice, guinea pigs, hamsters, amphibians, and fishes) and higher order invertebrates(e.g. cuttlefish, octopus, squid, lobster, bees, or American or German cockroaches)."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Human Subjects Research: All human subjects research (e.g.surveys, interviews, public engagement) you carry out must be done in accordance with relevant laws and regulations, as well as any institutional rules or guidance."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Antimicrobial Resistance: You must Check In before beginning experiments likely to increase the risk posed by antimicrobial resistance (e.g. by using novel resistance factors, adding known factors into new organisms, or resistance factors not previously used in your facility)."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Gene Drive: Gene Drive is not allowed in iGEM projects without a special exception from the Safety & Security Committee."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Coronavirus: Any work using live SARS-CoV-2 virus(the causative agent of COVID-19) must follow World Health Organization (WHO) guidance, in addition to all local and institutional rules. WHO guidance may be stricter than local safety rules. You must Check In before beginning experiments with live SARS-CoV-2 or parts from SARS-CoV-2."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Environmental Samples: As long as parts or organisms are not isolated from them, samples from the environment, the food industry, or other non- traditional suppliers may be used in your experiments You must Check In before using any parts organisms obtained from outside the lab or from non- traditional suppliers."}]}]},{"type":"element","tag":"h2","props":{"id":"project-safety"},"children":[{"type":"text","value":"Project safety"}]},{"type":"element","tag":"h3","props":{"id":"safety-of-project-design"},"children":[{"type":"text","value":"Safety of project design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Our project employs the insertion of RecA intein into T7 RNAP to detect endocrine disruption effects and utilizes the SOS response mechanism of Escherichia coli to detect DNA damage. Additionally, the LuxI/R system is introduced to achieve an AND gate detection of both effects. Furthermore, the Hrp gene regulation system and BsFbFp fluorescent gene are combined to amplify the transcriptional signal and ensure effective signal expression under anaerobic conditions; in the hardware section, we have designed a high-throughput platform for automated sample detection. Throughout our entire experimental process, our team has conducted experiments using qualified E. coli strains such as BL21, DH5a, and MG1655, as well as plasmid vectors pUC19 and pET28. These E. coli strains are classified as Biosafety Level I and have been handled in a sterile environment."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"According to the WHO Laboratory Biosafety Manual, the risks involved in our experiments are Level I (no risk or very low risk)."}]},{"type":"element","tag":"h3","props":{"id":"project-hardware-security"},"children":[{"type":"text","value":"Project Hardware Security"}]},{"type":"element","tag":"ul","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Disposable Reaction Vessels: We utilize disposable PCR tubes as reaction vessels, which means that there is no need for contact with the biological system at the start of the reaction, thus eliminating concerns about genetic modification organism contamination."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"No Direct Contact: Users only need to add the sample liquid to the reaction chamber; unless necessary, it is not recommended to open our device."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Sealed Containers: The reaction chamber is completely sealed, so unless the user intentionally breaks it, there will be no opportunity for the user to come into contact with the reagents inside."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Automated Control: All operations after adding the sample can be controlled automatically through a mobile app, without the need for manual operation of the device."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Modular Design: If the device is damaged, only the specific part needs to be replaced, without the need for disassembly."}]}]},{"type":"element","tag":"h3","props":{"id":"engineering-bacterial-system"},"children":[{"type":"text","value":"Engineering Bacterial System"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Our project utilizes Escherichia coli as an engineered strain, relying on genetically designed circuits to specifically identify trace amounts of endocrine disruptors and elicit a response. In this process, the engineered strain's response is susceptible to factors such as cellular status, physiological conditions, and environmental influences; moreover, there is a possibility of the substances produced by the reaction leaking into the environment."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"safety-hardware","src":"https://static.igem.wiki/teams/5358/safety/hardware.webp"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Our hardware is capable of addressing these challenges. The device is designed using a modular approach. We provide users with sealed reaction vessels, thereby minimizing the need for direct contact with chemical reagents. The device features a light-blocking cover and a constant temperature control system that provide our Escherichia coli with a stable, light-protected, and temperature-controlled environment to match their optimal working conditions."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To prevent contamination of our system and the environment, we have implemented the following designs:"}]},{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"The fluorescence detection module is covered with a black nylon protective sleeve to provide a dark chamber, reducing the interference of background light on the detection results."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"The cover is extended around to prevent substances produced by the reaction from escaping into the atmosphere, ensuring biosafety."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Disposable reaction vessels are used, and they are subjected to specialized biosafety treatment after the detection is completed."}]}]},{"type":"element","tag":"h2","props":{"id":"laboratory-security"},"children":[{"type":"text","value":"Laboratory security"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"All laboratories in China must be accredited by the Office of the Ministry of Environment in accordance with the standard "},{"type":"element","tag":"code","props":{"className":[]},"children":[{"type":"text","value":"LST EN ISO/IEC 17025:2018"}]},{"type":"text","value":". To ensure safety, team members must comply with all essential safety protocols before working in different laboratories. Prior to entering the lab, each team member is required to familiarize themselves with the experimental manual beforehand, and to receive training on experimental safety measures."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"All team members must familiar themselves with the safety features in the lab, such as"}]},{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Fire Extinguisher;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"First Aid Kit;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Biosafety Cabinet;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Flame Retardant Benches;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"the Nearest Safety Shower;"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Wastes after 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These substances are widely found in plastics, pesticides, industrial chemicals, and daily consumer goods. They enter the human body through the food chain or through direct contact, interfering with hormonal balance, and may cause reproductive health problems, developmental disorders, metabolic disorders and even some types of cancer.","body":{"type":"root","children":[{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Endocrine disruptors are a class of chemicals that can interfere with the normal function of the endocrine system, and they may mimic or inhibit the effects of natural hormones, thereby affecting the physiological processes of the body. These substances are widely found in plastics, pesticides, industrial chemicals, and daily consumer goods. They enter the human body through the food chain or through direct contact, interfering with hormonal balance, and may cause reproductive health problems, developmental disorders, metabolic disorders and even some types of cancer."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"When endocrine disruptors affect hormone activity, they may also induce cellular oxidative stress because they increase the generation of reactive oxygen species (ROS), highly reactive chemicals that damage cellular structures, including proteins, lipids, and DNA. Long-term oxidative stress accelerates cellular aging and may also lead to male infertility and the development of multiple chronic diseases. Thus, there is a close link between exposure to endocrine disruptors and cellular oxidative damage, a potential promoter to multiple health problems. Therefore, it is necessary to detect the endocrine interference effects of endocrine disruptors and oxidative damage effects in the relevant location samples."}]},{"type":"element","tag":"h2","props":{"id":"_1-puc19-preca-bsfbfp-bl21"},"children":[{"type":"text","value":"1. pUC19-PrecA-BsFbFp-BL21"}]},{"type":"element","tag":"h3","props":{"id":"strain-construction"},"children":[{"type":"text","value":"Strain construction"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We plan to design a portable microbial sensor, in which the sensor chamber will be maintained in a nearly anoxic state. Under anoxic conditions, the fluorescence intensity of EGFP diminishes, and its detection limit decreases; therefore, traditional EGFP is not the optimal choice for our application. We have constructed the PrecA-BsFbFP circuit, where BsFbFP is an anoxic fluorescent protein that exhibits fluorescence intensity nearly equivalent to that of EGFP under anoxic conditions, while significantly improving the detection limit. Consequently, we will utilize BsFbFP as the expression product of our engineered strain for detection characterization."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We obtained the PrecA-BsFbFp circuit and the recA-eGFP (hereafter referred to as wild) circuit through PCR.The gene circuits were ligated to the pUC19 vector using enzyme ligation and transformed into BL21 competent cells . The transformed cells were spread onto LB solid medium supplemented with ampicillin. Single colonies that grew were selected for amplification and sent to Jinweizhi (Suzhou) Biochemistry Co. for sequencing, and the remaining bacteria were preserved in glycerol."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/fig1.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/bsfbfp.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 1. Results of the enzyme digestion verification experiment for pUC19-PrecA-BsFbFP-BL21."}]}]},{"type":"element","tag":"h3","props":{"id":"characterization-of-experiments"},"children":[{"type":"text","value":"Characterization of experiments"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Firstly, we performed screening of the pUC19-PrecA-BsFbFp-BL21. The transformed wild-type bacteria were cultured on a shaker until the OD value reached 0.2, followed by the addition of NA for induction for 2 h. In aerobic conditions, BsFbFp is significantly less characterized than EGFP, while the RFU is close, and BsFbFp has a high detection limit, indicating that BsFbFp is an excellent choice for characterization in anaerobic conditions."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/o2-fluo.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/o2-rfu.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 2. Characterization results of BsFbFP and EGFP under anoxic and NA gradient injury conditions."}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We have characterized the pUC19-PrecA-BsFbFp-BL21 under anaerobic conditions.The transformed wild-type bacteria were cultured on a shaker until the OD value reached 0.2, followed by the addition of NA for induction for 2 h.As the concentration of NA increased, the fluorescence intensity of engineering strains increased accordingly. Several experiments proved that the RFU value increased with the concentration of NA in the anaerobic environment."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/10-rfu.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/10-rfu-more.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 3. Fluorescence intensity characterization of BsFbFP under NA gradient injury conditions in 10-lianpao chip cultures, expressed as RFU."}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Later, we compared the characterization difference between DH5α strain and BL21 strain, and showed that the BL21 strain has higher sensitivity to injury, reflecting its advantage as a representative strain."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/o2-fluo-more.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 4. Fluorescence characterization gradients of DH5α and BL21 under aerobic and NA gradient injury conditions."}]}]},{"type":"element","tag":"h2","props":{"id":"_2-pet28-preca-hrpr-hrps-phrpl-bsfbfp-bl21"},"children":[{"type":"text","value":"2. pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The gene circuits were ligated to the pET28 vector using enzyme ligation and transformed into BL21 competent cells . The transformed cells were spread onto LB solid medium supplemented with ampicillin.Preserved strains were stored in glycerol."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/hrp.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/2-enzyme-digestion.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 5. Results of the enzyme digestion verification experiment for pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21."}]}]},{"type":"element","tag":"h2","props":{"id":"_3-puc19-pbad-n-t7rnap-er-lbd-intein-c-t7rnap-bsfbfp"},"children":[{"type":"text","value":"3. pUC19-PBAD-N-T7RNAP-ER-LBD intein-C-T7RNAP-BsFbFp"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The gene sequencing conducted by our company has successfully validated the synthesis of the intein of the biological circuit."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/fig3.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/gene-squencing.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 6. Gene sequencing results of the ER-LBD intein."}]}]},{"type":"element","tag":"h2","props":{"id":"_4-psb1c3-pj23100-luxi-bl21-and-psb1c3-plux-luxr-egfp-bl21"},"children":[{"type":"text","value":"4. psB1C3-Pj23100-LuxI-BL21 and psB1C3-Plux-LuxR-EGFP-BL21"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/fig4.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We obtained by PCR the Pj233100, Plux-LuxR, LuxI, and EGFP and ligated it to the psB1C3 plasmid into E. coli BL21. The transformed cells were spread onto LB solid medium supplemented with chloramphenicol. Recombinants were used for characterization experiments and stored in glycerol."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/pcr.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 7. PCR products of the Pj23100-LuxI fragment and the Plux-LuxR-EGFP fragment."}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/lux-digestion.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 8. Enzyme digestion verification of psB1C3-Pj23100-LuxI-BL21 and Plux-LuxR-EGFP-BL21."}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/ligation.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 9. Enzyme Ligation Product of psB1C3-Pj23100-LuxI-BL21 and Plux-LuxR-EGFP-BL21."}]}]}],"toc":{"title":"","searchDepth":1,"depth":3,"links":[{"id":"_1-puc19-preca-bsfbfp-bl21","depth":2,"text":"1. pUC19-PrecA-BsFbFp-BL21","children":[{"id":"strain-construction","depth":3,"text":"Strain construction"},{"id":"characterization-of-experiments","depth":3,"text":"Characterization of experiments"}]},{"id":"_2-pet28-preca-hrpr-hrps-phrpl-bsfbfp-bl21","depth":2,"text":"2. pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21"},{"id":"_3-puc19-pbad-n-t7rnap-er-lbd-intein-c-t7rnap-bsfbfp","depth":2,"text":"3. pUC19-PBAD-N-T7RNAP-ER-LBD intein-C-T7RNAP-BsFbFp"},{"id":"_4-psb1c3-pj23100-luxi-bl21-and-psb1c3-plux-luxr-egfp-bl21","depth":2,"text":"4. psB1C3-Pj23100-LuxI-BL21 and psB1C3-Plux-LuxR-EGFP-BL21"}]}},"_type":"markdown","_id":"content:wetlab-results.md","_source":"content","_file":"wetlab-results.md","_stem":"wetlab-results","_extension":"md"}],"navigation":[{"title":"Hardware","_path":"/drylab-hardware"},{"title":"Integrated Human Practice","_path":"/hp-ihp"},{"title":"Models","_path":"/models"},{"title":"Description","_path":"/project-description"},{"title":"Project Engineering","_path":"/project-engineering"},{"title":"Safety","_path":"/project-safety"},{"title":"Experiment","_path":"/wetlab-experiment"},{"title":"Notebook","_path":"/wetlab-notebook"},{"title":"Parts","_path":"/wetlab-parts"},{"title":"Results","_path":"/wetlab-results"}]}
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+{"generatedAt":1727871091241,"generateTime":804,"contents":[{"_path":"/drylab-hardware","_dir":"","_draft":false,"_partial":false,"_locale":"","title":"Hardware","description":"This year, the core objective of the BIT team is to develop a initiative detection system for EDCs in aquatic environments based on E.coli biosensors. Traditional detection methods, such as chromatography and immunoassays, require cumbersome procedures like sampling, pre-treatment, and laboratory examination, in addition to expensive laboratory equipment (e.g., chromatographs, microplate reader) and professional experimental techniques. These methods are time-consuming and costly.","body":{"type":"root","children":[{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"This year, the core objective of the BIT team is to develop "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"a initiative detection system for EDCs in aquatic environments based on E.coli biosensors."}]},{"type":"text","value":" Traditional detection methods, such as chromatography and immunoassays, require cumbersome procedures like sampling, pre-treatment, and laboratory examination, in addition to expensive laboratory equipment (e.g., chromatographs, microplate reader) and professional experimental techniques. These methods are time-consuming and costly."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"As EDCs continue to threaten aquatic environments and the quality of drinking water for residents, it is crucial to achieve portable detection and rapid response to EDCs. To meet the requirement, our goal is to develop a low costing, highly integrated, compact, portable, and user-friendly hardware platform that can be combined with smartphone software to automate the cultivation of engineering bacteria and the analysis of fluorescence "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"detection"}]},{"type":"text","value":"."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To ensure that the hardware device truly meets the needs of system construction, we have adopted a typical engineering and project design approach, namely: "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"demand analysis, scheme proposal, feasibility verification, feedback , system improvement, and goal achievement."}]},{"type":"text","value":" This process is an iterative one, and after several cycles, it ultimately meets all the needs and objectives."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/1.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 1 Hardware Design Flowchart"}]}]},{"type":"element","tag":"h2","props":{"id":"demand-analysis"},"children":[{"type":"text","value":"Demand Analysis"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To ensure the stability and accuracy of "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"fluorescence detection"}]},{"type":"text","value":", it is necessary to standardize the detection process and use high sensitive detection units."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Standardization of the detection process requires a continuous and stable chemical and physical environment during the resuscitation of engineering bacteria and fluorescence detection. The standardization of the chemical environment is mainly ensured by the quantified operation of experimental steps (including control of sample volume and the number of engineering bacteria, control of the chemical composition and concentration of the culture medium), and the standardization of the physical environment is mainly ensured by the stability of light source, temperature, and humidity. Among them, light exposure problem has been solved by the sealed and light-proof nature of the device, and the humidity in the sealed environment is mainly determined by temperature. The standardization of the detection process mainly aims to achieve "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"constant temperature control"}]},{"type":"text","value":" of the hardware device."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Since the concentration of "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"EDCs"}]},{"type":"text","value":" in the detection samples is very low，which also refer to the low intensity of fluorescence, coupled with the portability requirements of the equipment, the detection method required must be high sensitive and can be integrated into small devices. Common chemical detection methods cannot meet the requirements, so adopting a more sensitive, portable, and efficient detection method is crucial for the realization of our POCT system."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In order to enable smartphone software to automate the control of hardware devices and visualize the detection results, the construction of hardware devices needs to consider the transmission of data between software and hardware."}]},{"type":"element","tag":"h2","props":{"id":"scheme-proposal"},"children":[{"type":"text","value":"Scheme Proposal"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/2.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 2 First Edition Design Scheme"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/3.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 3 Circuit Diagram"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In the construction of the "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"temperature control module"}]},{"type":"text","value":", we employed an NTC thermistor as the "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"temperature sensor"}]},{"type":"text","value":", utilizing heating FILM and fan as the heat and cold sources respectively. To achieve "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"PID regulation"}]},{"type":"text","value":" for "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"temperature sensing and control"}]},{"type":"text","value":", we used the PWM wave output from the "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"TB6612FNG motor drive"}]},{"type":"text","value":" to adjust the power of the fan and heating FILM, thereby maintaining dynamic temperature stability."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"For the selection of the detection scheme, we comprehensively considered the detection accuracy and the portability of the device, ultimately choosing the "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"fluorescence detection module"}]},{"type":"text","value":". We initially selected the AS7341 as "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"fluorescence sensor"}]},{"type":"text","value":" "},{"type":"element","tag":"span","props":{},"children":[{"type":"text","value":"1"}]},{"type":"text","value":". Concurrently, to ensure consistent fluorescence generation, we redesigned and optimized the optical path."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"After the marked fluorescence is excited by a blue LED, the fluorescence sensor collect and record real-time fluorescence signals, which can be visualized on the mobile software via a "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"communication module"}]},{"type":"text","value":"."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"For the communication module, we used the HC-05 Bluetooth module to connect the hardware system with the mobile software, enabling real-time monitoring of the hardware device's operating status and feedback of the detection results."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In the initial version of the scheme, we used an STM32 chip as the "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"microcontroller Unit(MCU)"}]},{"type":"text","value":", connecting temperature control module, fluorescence detection module, and Bluetooth communication module, ensuring the normal operation of the device through programming instructions."}]},{"type":"element","tag":"mdc-wrap","props":{":class":"table-fig"},"children":[{"type":"element","tag":"table","props":{},"children":[{"type":"element","tag":"thead","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"th","props":{"align":"center"},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/4.png"},"children":[]}]},{"type":"element","tag":"th","props":{"align":"center"},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/5.png"},"children":[]}]}]}]},{"type":"element","tag":"tbody","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{"align":"center"},"children":[{"type":"text","value":"Figure 5:  First Edition Implementation Scheme"}]},{"type":"element","tag":"td","props":{"align":"center"},"children":[{"type":"text","value":"Figure 6: First Edition Equipment Model Diagram"}]}]}]}]}]},{"type":"element","tag":"h3","props":{"id":"feasibility-verification"},"children":[{"type":"text","value":"Feasibility Verification"}]},{"type":"element","tag":"h3","props":{"id":"temperature-stability-verification"},"children":[{"type":"text","value":"Temperature Stability Verification"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To verify the constant temperature effect of the temperature control system, we designed a temperature stability test experiment. When the temperature first reached 37°C, the temperature was recorded every 10 seconds for an additional 60 seconds. From the results shown in Figure 6, it can be concluded that the absolute difference between the average temperature and the set temperature was no greater than 1.1°C, and the temperature curve consistently fluctuated around 37°C. This indicates that the temperature control system can achieve dynamic temperature regulation, capable of maintaining the temperature close to 37°C. However, this temperature control method generates relatively large temperature fluctuations."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["flex","gap-4"]},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/6.png"},"children":[]},{"type":"text","value":" "},{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/7.png"},"children":[]}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 7 Measurement Results of  Initial Scheme's Temperature Stability"}]}]},{"type":"element","tag":"h3","props":{"id":"gradient-fluoresce-in-sodium-fluorescence-detection-verification"},"children":[{"type":"text","value":"Gradient Fluoresce in Sodium Fluorescence Detection Verification"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The AS7341 fluorescence sensor, by integrating filters of different wavelength bands, achieves the function of detecting light intensity (Lux) at specific wavelengths, which is equivalent to detecting the corresponding fluorescence intensity. To verify whether this sensor can correctly reflect the signal from the sample, we designed an experiment to explore the relationship between the concentration gradient of fluoresce in sodium and Lux. We prepared a series of fluoresce in sodium solutions with different gradients in a 2mL centrifuge tube for signal collection using the AS734. The results shown in Figure 8 indicate that the measured Lux values have a good linear correlation with the concentration gradient of fluoresce in sodium, demonstrating that our fluorescence detection module can accurately reflect the concentration of substances and is capable of conducting fluorescence detection."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/8.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 8 Initial Scheme's Fluorescence Verification Fitting Curve"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The above feasibility verification demonstrates that the temperature control module and fluorescence detection module designed in the first version of the scheme can meet the design requirements and are feasible."}]},{"type":"element","tag":"h2","props":{"id":"feedback"},"children":[{"type":"text","value":"Feedback"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We engaged in in-depth discussions with the biology team, design team, and professional instructors regarding our hardware design, obtaining extremely valuable feedback. This feedback is instrumental in guiding our further improvements."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"1.MCU:"}]},{"type":"text","value":" The STM32 requires an external wireless communication module to implement Bluetooth functionality, which increases the complexity of the design."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"2.Temperature Control Module:"}]},{"type":"text","value":" Using the TB6612FNG to drive temperature control presents issues with heat dissipation and difficulties in achieving integration."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"3.Detection Module:"}]},{"type":"text","value":" The scheme of combining a fluorescence sensor with an optical path tends to have fluctuations in the excitation light source, and the optical path alignment is challenging and susceptible to environmental interference."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"4.Biological Requirements:"}]},{"type":"text","value":" The current design only allows for single detection, and frequent changes in the "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"reaction tube"}]},{"type":"text","value":" can affect the stability of the detection environment, ultimately leading to inaccurate results."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In response to the issues raised and the suggestions from the instructors, we have made improvements based on the original design."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/9.png"},"children":[]}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 9 Improved Design Scheme"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Firstly, we replaced the STM32F103C8T6 with the Arduino Nano ESP32 chip as the MCU. The ESP32 features built-in Bluetooth communication capabilities and a rich set of peripheral interfaces. It supports the CMake and Ninja build systems, which will greatly simplify the design complexity. Its widespread adoption globally will also make it more universal and laying a foundation for future teams looking to design similar devices. For temperature control and detection, we replaced the original scheme with the TCM1040 temperature control module and the FM-4A digital fluorescence detection module from LIDE Technology, respectively. Compared to the original scheme, these modules offer more integrated features, further enhancing the accuracy and stability of the platform."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/10.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 10 Improved Design Circuit Diagram"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To meet the demand for high-throughput detection, we proposed two solutions: a microfluidic chip rotation detection platform and a linear slide rail detection platform. After modeling analysis and feasibility analysis, we ultimately chose the linear slide rail detection platform and designed a brand-new enclosure for it."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/11.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 11 Implementation Plan of the New Equipment"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/engineering-success/new.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 12 Model Diagram of the New Equipment"}]}]},{"type":"element","tag":"h3","props":{"id":"goal-achievement"},"children":[{"type":"text","value":"Goal Achievement"}]},{"type":"element","tag":"h4","props":{"id":"final-product"},"children":[{"type":"text","value":"Final Product"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The final system is primarily divided into a "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"sampling area"}]},{"type":"text","value":" and a "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"detection area."}]},{"type":"text","value":" In actual use, the freeze-dried engineering bacteria will be mixed with multiple samples to be tested in separate reaction chambers for cultivation, awaiting detection.Upon receiving operational commands from the software, the device rapidly heats the aluminum alloy temperature-controlled slot in "},{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"the sampling area"}]},{"type":"text","value":" and maintains a constant temperature, while also providing real-time feedback of the temperature and the operational status of the system to the mobile software interface via Bluetooth. Concurrently, the fluorescence detection module in the detection area begins to sequentially detect the fluorescence signals of each reaction chamber along the slide rail and provides real-time feedback to the software's visual interface. When the engineering bacteria grow to a certain extent, the intensity of the fluorescence signal will reflect the degree of endocrine disruptor effects."}]},{"type":"element","tag":"h5","props":{"id":"functional-testing"},"children":[{"type":"text","value":"Functional Testing"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Temperature Control Capability Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We conducted another temperature stability test on the latest version of the system. The results shown in Figure 12 indicate that the absolute difference between the average temperature and the set temperature is less than 0.3°C, suggesting that the stability of the improved temperature control system has been further enhanced. This can well meet practical requirements."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["flex","gap-4"]},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/13.png"},"children":[]},{"type":"text","value":" "},{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/14.png"},"children":[]}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 13 Measurement Results of Temperature Fluctuation"}]}]},{"type":"element","tag":"h5","props":{"id":"gradient-fluorescein-sodium-fluorescence-detection-verification"},"children":[{"type":"text","value":"Gradient Fluorescein Sodium Fluorescence Detection Verification"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The fluorescence validation test of the latest equipment shows that the tested Relative Fluorescence Units (RFU) values have a good linear relationship with the concentration gradient of fluorescein sodium. This indicates that the new fluorescence detection module can effectively reflect the concentration of substances, meeting the usage requirements."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/hardware/15.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 14 Fluorescence Verification Experiment Fitting Curve"}]}]},{"type":"element","tag":"h2","props":{"id":"android-software"},"children":[{"type":"text","value":"Android Software"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We have conducted functional testing on the already designed software. Initially, the software is imported onto an Android phone and the Bluetooth module is connected to check for a normal connection. The software is then opened to inspect whether the UI (User Interface) design is complete. Subsequently, the signals received from the module are used to test the graph plotting functionality to ensure it operates correctly, and the system is optimized based on the results."}]},{"type":"element","tag":"h2","props":{"id":"data-reception-verification"},"children":[{"type":"text","value":"Data Reception Verification"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Signals for initiation are transmitted from the mobile phone to the MCU via Bluetooth. As can be seen from the diagram, the hardware successfully starts up and returns a designated identifier."}]},{"type":"element","tag":"h2","props":{"id":"data-plotting-verification"},"children":[{"type":"text","value":"Data Plotting Verification"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"During operation, the MCU sends data to the mobile phone via Bluetooth. The mobile phone then parses this data and plots graphs of time-temperature and time-fluorescence intensity. The diagrams indicate that the program has successfully implemented its functionalities."}]},{"type":"element","tag":"h2","props":{"id":"improvement"},"children":[{"type":"text","value":"Improvement"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Due to the addition of a linear slide rail for the movement of fluorescence detection in the second version of the hardware design, the signals obtained from the fluorescence detection have also changed. Therefore, the program is required to implement the ability to distinguish between the fluorescence intensity pulse peaks of different channels and to obtain their respective maximum values (theoretically, the fluorescence intensity at the bottom of the tube) for plotting."}]},{"type":"element","tag":"h2","props":{"id":"array-splitting-and-plotting-verification"},"children":[{"type":"text","value":"Array Splitting and Plotting Verification"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We can observe a set of data with multiple pulse peaks returned by the MCU. The software successfully splits this data into several different arrays and obtains the maximum value from each array for plotting."}]},{"type":"element","tag":"h2","props":{"id":"summary"},"children":[{"type":"text","value":"Summary"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The final product and its testing demonstrate that the proactive detection system for EDCs we have developed is compact, portable, and user-friendly. It effectively controls the standardization of cultivation conditions, enabling rapid screening and assessment of EDCs in wastewater, significantly reducing reliance on laboratory equipment and professional personnel. Additionally, the system's high-throughput operation allows for the simultaneous detection of multiple samples, thereby conducting sample testing more efficiently."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In designing the hardware, we employed engineering and project design approaches, taking into account target requirements and user feedback. Our instructor, Professor Li Xiaoqiong, recommended replacing the STM32 chip with the ESP32 chip, which will aid in enhancing the readability and versatility of the design. During testing and actual use, we collected feedback from operators and utilized a high-throughput platform, facilitating users to test multiple samples conveniently."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To facilitate user operation, we have added detailed user instructions in the App and will record demonstration videos for ease of use. At the same time, we have organized detailed system design blueprints and modeling files, as well as detailed component selection and manufacturing methods. You can contact us on the contribution page to obtain the relevant documents, thereby assisting other participants in replicating our device. Our main controller and major systems utilize common integrated units, while other parts are made using industrial processing and 3D printing techniques, making them easy to replicate for reference and implementation by other teams."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["text-2xl"]},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"text","value":"References"}]}]}]},{"type":"element","tag":"mdc-wrap","props":{":class":"reference"},"children":[{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Wang Y, Chen H, Lin K, et al. Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device"},{"type":"element","tag":"span","props":{},"children":[{"type":"text","value":"j"}]},{"type":"text","value":". Nature Communications, 2024, 15(1):3279."}]}]}]}],"toc":{"title":"","searchDepth":1,"depth":3,"links":[{"id":"demand-analysis","depth":2,"text":"Demand Analysis"},{"id":"scheme-proposal","depth":2,"text":"Scheme Proposal","children":[{"id":"feasibility-verification","depth":3,"text":"Feasibility Verification"},{"id":"temperature-stability-verification","depth":3,"text":"Temperature Stability Verification"},{"id":"gradient-fluoresce-in-sodium-fluorescence-detection-verification","depth":3,"text":"Gradient Fluoresce in Sodium Fluorescence Detection Verification"}]},{"id":"feedback","depth":2,"text":"Feedback","children":[{"id":"goal-achievement","depth":3,"text":"Goal Achievement","children":[{"id":"final-product","depth":4,"text":"Final Product"}]}]},{"id":"android-software","depth":2,"text":"Android Software"},{"id":"data-reception-verification","depth":2,"text":"Data Reception Verification"},{"id":"data-plotting-verification","depth":2,"text":"Data Plotting Verification"},{"id":"improvement","depth":2,"text":"Improvement"},{"id":"array-splitting-and-plotting-verification","depth":2,"text":"Array Splitting and Plotting Verification"},{"id":"summary","depth":2,"text":"Summary"}]}},"_type":"markdown","_id":"content:drylab-hardware.md","_source":"content","_file":"drylab-hardware.md","_stem":"drylab-hardware","_extension":"md"},{"_path":"/hp-ihp","_dir":"","_draft":false,"_partial":false,"_locale":"","title":"Integrated Human Practice","description":"","body":{"type":"root","children":[{"type":"element","tag":"h2","props":{"id":"overview"},"children":[{"type":"text","value":"Overview"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The core of HP is to present project designs and products to the community and stakeholders, get feedback, integrate feedback into the project, and constantly adjust and modify the project according to the guidance of the feedback. This year, we proposed the \"BRIEF\" periodic iteration method to complete IHP. The aim is to ensure that projects meet practical needs, continuously improve project performance through feedback, meet the needs of stakeholders, and contribute to society in order to build projects that are good and responsible for the world."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/ihp/brief.jpg"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The \"BRIEF\" concept consists of five phases of IHP implementation, They are Brainstorming, Researching, Insight Integrating, Executing and Adjusting, and Feedback Recollecting five aspects. Among them, Executing and Adjusting the project is a bridge connecting Brainstorming, Researching, Insight Integrating and Feedback Recollecting. Throughout IHP, we build a cycle of finding meaning, reaching out, and connecting our projects to the world."}]},{"type":"element","tag":"h2","props":{"id":"brainstorming"},"children":[{"type":"text","value":"Brainstorming"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Our team is focusing on detecting new pollutants this year. At the beginning of the project, we carried out extensive research on new pollutants, and focused on endocrine disruptors, microplastics, antibiotics, persistent pollutants four, because of their greater harm and detection methods are expensive and difficult to operate."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Since then, endocrine disruptors have been screened according to the difficulty of detection, the harm to the environment and the significance of positive results. In the traditional evaluation system of endocrine disruptors, most of the main methods are to detect their binding with hormone receptors. However, due to the extensive and complex toxic effects of endocrine disruptors and their metabolites on organisms, the traditional detection methods are obviously inadequate."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The result of our brainstorming is to build a gate-based multi-toxicological screening sensor that can simultaneously detect endocrine disrupting effects and DNA damage effects in factory and enterprise products, or unknown samples in the environment."}]},{"type":"element","tag":"h2","props":{"id":"researching"},"children":[{"type":"text","value":"Researching"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"After determining the study of endocrine disruptors, we screened out methods that could detect endocrine disruptors through literature research and conducted feasibility analysis. Finally, a method was established to detect endocrine disruptors by constructing hormone analogues sensitive peptides and coupled with Escherichia coli SOS oxidative damage response system and hrp amplifier for detecting DNA damage effects to construct a gated multi-toxicological effects screening sensor design. In addition, we investigated the contained peptide sequence and found that the number of bases was too large, which would cause a heavy burden on Escherichia coli. Therefore, the components for detecting the two effects were divided into two bacteria, and the LuxI/LuxR system was used for communication. Biosensors use fluorescent protein reports.\nThis is the initial design of our project."}]},{"type":"element","tag":"h2","props":{"id":"insight-integrating"},"children":[{"type":"text","value":"Insight Integrating"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In order to identify the social needs of our project with stakeholders, we contacted environmental testing companies, government departments, factories and wastewater treatment plants to ask about the needs and status of pollutant monitoring. In addition, in order to enhance the professional and scientific nature of the project, we also consult with researchers in related fields to adjust our research direction and research content."}]},{"type":"element","tag":"h3","props":{"id":"government-department"},"children":[{"type":"text","value":"Government Department"}]},{"type":"element","tag":"h4","props":{"id":"bureau-of-ecology-environment-and-environmental-protection"},"children":[{"type":"text","value":"Bureau of Ecology Environment and Environmental Protection"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"On May 23, 2024, our team members approached Ms. Luo, an official of Chongqing Ecological Environment and Environmental Protection Bureau, to consult the social needs of our project.\nFirst of all, we consulted the definition method, detection and registration process and control measures of endocrine disruptors. Luo pointed out that after confirming that a substance is polluting, the government departments will commission professional technical institutions to conduct research and investigation every year, and if the result is ultra-ultra-trace, relatively safe, no further measures will be taken. But if the concentration is high, the government will take measures and test the water quality of the test site or its source to track its possible source. In the analysis of new pollutants, including endocrine disruptors, government departments will conduct toxicological tests on chemical substances based on cutting-edge international research to determine that a substance may be harmful to the human body. This is a very important part of the testing process, because the government departments will concentrate the samples, which will lead to a variety of substances will be detected, and only when these substances are associated with toxicological effects and confirmed to be harmful to the human body, it is necessary to define endocrine disruptors and monitor. In terms of control, the government has corresponding emission standards for well-known endocrine disruptors; For new pollutants such as endocrine disruptors detected, the government will continue to confirm whether they belong to the emission substances already on the control list. For the situation where the pollutant index exceeds the standard, the government department will require the enterprise to take corresponding treatment measures and implement management measures such as regular review in the follow-up to supervise the rectification results."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"As for endocrine disruptors, Luo pointed out that in some studies, all organic compounds were classified as endocrine disruptors under investigation, which is not accurate; The current approach is to study each emerging substance independently and then classify it. The test site of endocrine disruptors is generally in other important water bodies such as drinking water sources, livestock and poultry farms and chemical plants and other emission sources."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Secondly, we proposed a method to determine whether the water quality contains endocrine disruptors by using the biosensor constructed by the engineering bacteria that specifically recognizes endocrine disruptors. Luo first confirmed that in the environmental field, the emergency equipment launched abroad in the past few years does include microbial sensors and fluorescence detection technology to deal with sudden emergency environmental accidents. For endocrine disruptors, the government does not include them in routine testing tasks, but they are regularly included in testing efforts. The government prefers to use more accurate tests to detect endocrine disruptors. Therefore, from this point of view, improving the accuracy and sensitivity of the detection is also very beneficial and important."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In short, Luo affirmed our project's detection method of using highly sensitive and specific biosensors for endocrine disruptors in the environment, which is very necessary and beneficial for government departments to detect endocrine disruptors, and our project is in line with the needs of government departments."}]},{"type":"element","tag":"h3","props":{"id":"environmental-testing-company"},"children":[{"type":"text","value":"Environmental Testing Company"}]},{"type":"element","tag":"h4","props":{"id":"shanghai-environmental-monitoring-technology-co-ltd"},"children":[{"type":"text","value":"Shanghai Environmental Monitoring Technology Co., Ltd"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/ihp/guo.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In order to continue to understand the definition method and testing registration process of new pollutants, on August 1, 2024, we found Mr. Guo, chief engineer of Shanghai Environmental Monitoring Technology Co., Ltd. for consultation."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Guo pointed out that the determination of pollutants is first based on those already found by the country, and their emissions often exceed the prescribed standards, while classification and the formulation of standards are needed. The study of these pollutants needs to determine whether they have harmful effects on the human body through effect analysis, toxicology analysis and process analysis. Secondly, the use of foreign literature research combined with the actual situation in China to develop a standardized detection process to find endocrine disruptors. The definition of most new pollutants, especially endocrine disruptors, is that they are first found to be harmful to human health, and then they are analyzed in detail."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"For the analysis standards of new pollutants such as endocrine disruptors, Guo mentioned that with the development of industry, some new substances will be continuously studied, but whether it is harmful to the human body needs to be determined through a scientific judgment process, and if it is found to be harmful to the human body, it needs to be immediately included in the supervision. According to the requirements of the state, Shanghai has put the new pollutants to be studied into the supervision. Both testing organizations and industry systems are constantly developing methods to detect new pollutants, including endocrine disruptors, and current detection instruments are sophisticated and expensive."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In the subsequent improvement process, the hardware device has undergone a simplified design to achieve the above design goals of portability and miniaturization."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In the communication with Luo and Guo, they both affirmed our thinking and importance of detecting new pollutants such as endocrine pollutants through toxicological effects."}]},{"type":"element","tag":"h3","props":{"id":"factory-and-enterprise"},"children":[{"type":"text","value":"Factory and Enterprise"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Our next step is to understand the current status of the detection and analysis of endocrine disruptors from the perspective of relevant units and companies, as well as the specific needs currently existing, and ask whether there are applicable scenarios for the project. In accordance with Luo's previous guidance, we found a sewage plant and a water plant in Shanghai on August 1, 2024."}]},{"type":"element","tag":"h4","props":{"id":"shanghai-taihe-wastewater-treatment-plant"},"children":[{"type":"text","value":"Shanghai Taihe Wastewater Treatment Plant"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/ihp/liu.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We have found Mr. Liu, director of Shanghai Taihe Sewage Plant. His reply to the above question is that the main pollutants under supervision of the sewage plant are Cod, Bod, ammonia, nitrogen and phosphorus. However, the pollutants under supervision of the sewage plant are to send water samples to testing institutions for testing, and domestic sewage involves few substances with toxicological properties."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"For our project, Mr. Liu said that due to the above reasons, the emergence of relevant technologies is temporarily lacking. Therefore, we should reflect on the use cases of our own projects and focus on providing services to government departments and environmental monitoring agencies."}]},{"type":"element","tag":"h4","props":{"id":"shanghai-nanhui-water-plant"},"children":[{"type":"text","value":"Shanghai Nanhui Water Plant"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/ihp/ma.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In the drinking water industry, we found Mr. Ma, director of Shanghai Nanhui Water Plant, for consultation. In response to the above problems, the director pointed out that the detection of new pollutants in the drinking water industry, including endocrine disruptors, must use more expensive instruments, and the detection requirements and operations are more complicated; There is a need for emergency detection of source water for the use of biosensors. In view of our team's project, the factory director put forward that it can be applied in the rapid emergency monitoring of the above source water, and put forward the suggestions and technical requirements of our products for both qualitative and quantitative testing of drinking water source water."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Therefore, for factories and enterprises, our project has a demand in the rapid emergency monitoring of drinking water source water, and we need to design a gate-based multi-toxicological effect screening sensor that can carry out qualitative and quantitative analysis on the basis of the project and is equipped with portable detection equipment."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In short, the need for endocrine disruptor testing that we investigated is actually confirmed. At present, the detection of new pollutants including endocrine disruptors is more passive, and the method is more heavy on toxicological analysis, and has high cost and cumbersome operation. Therefore, there is a real need for a highly sensitive and specific detection of endocrine disruption and DNA damage in unknown samples, two important toxicological effects, and the hardware of such sensors must be portable and miniaturized."},{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/ihp/projectpropose.png"},"children":[]}]},{"type":"element","tag":"h3","props":{"id":"professional-research-personnel"},"children":[{"type":"text","value":"Professional research personnel"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/ihp/yang.jpg"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We had an in-depth discussion on Modeling with Professor Yang Guanghui of China Agricultural University. He first gave a positive opinion of our idea. As the latest achievement in the field of bioinformatics, Alphafold3 has a high degree of confidence in the prediction of protein structure function, which can effectively guide our research in synthetic biology. But he also points out that the number and length of hydrogen bonds alone cannot accurately measure how folded a protein domain is. The main force in protein folding is hydrophobic interaction. However, there are no effective means to quantitatively measure the degree of protein folding and the strength of hydrophobic interaction. He encouraged us to work in this direction, hoping that we would be able to measure protein folding from other angles."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Inspired by this, and after subsequent investigation, we will use Chimera1.13 software to analyze the hydrophobic interaction of the protein conformation obtained by molecular dynamics simulation (MDS), and measure the hydrophobic interaction intensity by comparing the hydrophobic potential around the recAN end and the C-end, so as to compare the degree of folding of this domain in the protein"}]},{"type":"element","tag":"h2","props":{"id":"feedback-recollecting"},"children":[{"type":"text","value":"Feedback Recollecting"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We plan to bring the prototype design to endocrine disruptor experts and previous stakeholders, and continue to receive their comments and feedback to continue to adapt our project; At the same time, we will also find new innovation points and needs from the feedback, so as to launch a new round of \"BRIEF\" cycle in the future and complete the closed loop."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"","src":"https://static.igem.wiki/teams/5358/ihp/executingandadjusting.png"},"children":[]}]}],"toc":{"title":"","searchDepth":1,"depth":3,"links":[{"id":"overview","depth":2,"text":"Overview"},{"id":"brainstorming","depth":2,"text":"Brainstorming"},{"id":"researching","depth":2,"text":"Researching"},{"id":"insight-integrating","depth":2,"text":"Insight Integrating","children":[{"id":"government-department","depth":3,"text":"Government Department","children":[{"id":"bureau-of-ecology-environment-and-environmental-protection","depth":4,"text":"Bureau of Ecology Environment and Environmental Protection"}]},{"id":"environmental-testing-company","depth":3,"text":"Environmental Testing Company","children":[{"id":"shanghai-environmental-monitoring-technology-co-ltd","depth":4,"text":"Shanghai Environmental Monitoring Technology Co., Ltd"}]},{"id":"factory-and-enterprise","depth":3,"text":"Factory and Enterprise","children":[{"id":"shanghai-taihe-wastewater-treatment-plant","depth":4,"text":"Shanghai Taihe Wastewater Treatment Plant"},{"id":"shanghai-nanhui-water-plant","depth":4,"text":"Shanghai Nanhui Water Plant"}]},{"id":"professional-research-personnel","depth":3,"text":"Professional research personnel"}]},{"id":"feedback-recollecting","depth":2,"text":"Feedback Recollecting"}]}},"_type":"markdown","_id":"content:hp-ihp.md","_source":"content","_file":"hp-ihp.md","_stem":"hp-ihp","_extension":"md"},{"_path":"/project-description","_dir":"","_draft":false,"_partial":false,"_locale":"","title":"Description","description":"","body":{"type":"root","children":[{"type":"element","tag":"h2","props":{"id":"background"},"children":[{"type":"text","value":"Background"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Environmental endocrine disruptors (EDCs) are a class of chemical substances that can mimic or block the effects of hormones, affecting the normal development of organisms. These substances include DDT, dioxins, and phthalates, among others."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"..."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"DDT is an insecticide that was once widely used, but due to its harmful effects on the reproductive and nervous systems of organisms, as well as its potential carcinogenicity, its use has been restricted in many countries since the 1970s and 1980s. However, as these substances have already entered the environment in large quantities, their negative effects persist even though their production has stopped for many years. They accumulate in the atmosphere, soil, water bodies, and organisms, posing a natural hazard."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In 2010, the direct social and economic loss caused by exposure to phthalate chemicals in China reached approximately 57.2 billion yuan, with the burden of male infertility being the most severe. Further calculations indicate that the total medical cost for all known EDCs exposure in China is about 429.4 billion yuan, accounting for approximately 1.07% of the annual GDP."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"..."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"All these indicate that we must pay attention to these potential dangers and have a more comprehensive understanding and early screening of environmental endocrine disruptors. Nowadays, with the deepening research on environmental endocrine disruptors, more and more studies are beginning to focus on the relationship between endocrine disruptors and their metabolites and other toxic effects. This superposition of multiple effects provides clues to revealing the molecular mechanisms underlying many diseases."}]},{"type":"element","tag":"h2","props":{"id":"problem"},"children":[{"type":"text","value":"Problem"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In fact, the role of environmental endocrine disruptors in organisms is complex, and the inference of their adverse effects needs to emphasize the adverse outcome pathway (AOP pathway, which describes the logical derivation of causally related events at different levels of biological tissues, leading to adverse effects on human or wildlife health). This requires us to describe the molecular mechanisms behind the impact comprehensively."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Currently, the methods for detecting endocrine disruptors using biological effects mainly include competitive ligand binding assays, fused cell aggregation experiments, and animal cell proliferation analysis experiments. These methods can only evaluate the endocrine disruption effect through the binding effect of hormone receptors when analyzing endocrine disruptors, and cannot judge the involvement of other effects behind the biological damage caused by endocrine disruptors, therefore, they have limitations."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"More and more literature has proven the important role of oxidative damage effects in the damage caused by environmental endocrine disruptors to organisms. For example, exposure to various parabens (PBs) and their metabolites can increase the level of oxidative stress, and the superposition of oxidative damage and endocrine disruption increases the risk of impaired reproductive capacity in organisms. The collaborative analysis of environmental endocrine disruption effects and oxidative damage effects can help us predict the pathways of action of environmental endocrine disruptors on organism damage, thereby assisting us in having a clearer understanding of their damage consequences and providing a reference for regulatory authorities to implement targeted regulatory policies."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Our project is based on this concept and aims to construct microbial sensors through synthetic biology methods to provide multiple detection lines for the assessment of endocrine disruption effects and oxidative damage effects. Not only can we achieve the separate detection of the two effects, but we can also realize the collaborative analysis of the two effects through logic gate detection lines, achieving a rapid, convenient, and comprehensive assessment of environmental endocrine disruptors. Conduct more in-depth research and discussion on the impact mechanism of environmental endocrine disruptors."}]},{"type":"element","tag":"h2","props":{"id":"inspiration"},"children":[{"type":"text","value":"Inspiration"}]},{"type":"element","tag":"h3","props":{"id":"biology"},"children":[{"type":"text","value":"biology"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Initially, we planned to develop a biosensor for detecting environmental endocrine disruptors. To achieve this goal, we studied the characteristics and mechanisms of action of environmental endocrine disruptors and researched the methods for detecting them. Intein peptides are protein analogs found in self-splicing RNA introns, and their insertion can lead to the inactivation of the host protein. Typically, they excise themselves from the host cell after translation, restoring the activity of the host protein. By replacing the homing endonuclease domain in the intein peptide with a hormone receptor binding domain, we can construct a hormone-sensitive intein peptide, which can be used to create recognition elements for endocrine disruption effects. We plan to construct an estrogen-sensitive intein peptide and insert it into T7 RNA Polymerase. T7 RNA Polymerase can specifically activate the T7 promoter, thus regulating the downstream circuit to express the reporter gene upon detection of estrogen mimics through the T7 promoter."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Subsequently, through more in-depth researches, it was found that the effects of environmental endocrine disruptors in organisms are complex. An increasing number of studies have demonstrated the significant role of oxidative stress in the damage caused by environmental endocrine disruptors. For example, exposure to various paraben compounds (PBs) and their metabolites can increase oxidative stress levels. The combined oxidative damage and endocrine disruption can heighten the risk of impaired reproductive capability in organisms. A synergistic analysis of the effects of environmental endocrine disruption and oxidative damage can help us predict the pathways through which these disruptors cause harm to organisms, thereby assisting us in gaining a clearer understanding of their damaging consequences. Therefore, we plan to incorporate DNA damage detection, designing an and gate detection circuit capable of simultaneously detecting endocrine disruption effects and DNA oxidative damage effects."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"DNA damage detection is based on the SOS response mechanism in "},{"type":"element","tag":"em","props":{},"children":[{"type":"text","value":"Escherichia coli"}]},{"type":"text","value":", which is an inducible DNA repair system activated when bacterial DNA is damaged. When bacteria detect DNA damage, the expression levels of genes associated with the SOS response mechanism significantly increase, leading to the expression of proteases that relieve the repression of target proteins, initiating the repair process. We chose the recA promoter to detect DNA damage, when bacteria sense DNA damage, the expression level of this promoter rises significantly, thereby activating downstream reporter genes."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To construct the logic gate, we introduced the hrp system, which consists of the hrpR and hrpS genes along with the HrpL promoter regulated by the sigma54 factor. The hrpL promoter is activated only when both hrpR and hrpS genes are expressed simultaneously."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We plan to insert the hrpR gene downstream of the P recA promoter, which will be activated in response to DNA damage effects; the hrpS gene will be inserted downstream of the P T7 promoter, which will be activated after the peptide-specific binding domain binds to estrogen analogs. The reporter gene egfp will be inserted downstream of the P hrpL promoter, resulting in a two-input AND gate circuit."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"However, the entire circuit is too large, posing significant challenges to the stability of the plasmid and the burden on the engineered bacteria. Therefore, we plan to divide the entire circuit into two parts, each constructed in different engineered bacteria, inserting new components to enable signal transmission between the two engineered bacterias."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Throughout the system, we have always planned to use the egfp gene as the reporter gene. However, this gene requires the presence of oxygen molecules as a cofactor for its luminescence, while the cultivation chamber of our designed hardware is in a low-oxygen environment. As a result, we tested a new reporter gene, BsFbFp, which can express significantly enhanced in vivo fluorescence in both aerobic and anaerobic environments, ensuring that the engineered bacteria can effectively express fluorescent signals in low-oxygen conditions."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Thus, the construction of the biological part of our circuit has been completed."}]},{"type":"element","tag":"h3","props":{"id":"hardware"},"children":[{"type":"text","value":"Hardware"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Point of Care Testing (POCT) is a form of rapid on-site testing/near-patient testing method which has been particularly impactful and is playing an increasingly vital role in the fields of medical diagnostics and environmental health monitoring due to its speed, convenience, and ease of operation. Traditional water contaminant detection technologies are often constrained by the need for costly equipment and a professional laboratory environment. In contrast, novel biosensor detection devices based on compact POCT equipment, when connected to an interactive app for operational control and visual data analysis, can effectively facilitate the rapid and efficient screening of emerging waterborne pollutants. This approach holds broad prospects for application in the field of environmental monitoring and assessment."}]},{"type":"element","tag":"h2","props":{"id":"solution"},"children":[{"type":"text","value":"Solution"}]},{"type":"element","tag":"h3","props":{"id":"biology-1"},"children":[{"type":"text","value":"biology"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"To achieve signal communication between the two engineered bacteria, we studied the bacterial quorum sensing mechanism. This mechanism allows bacteria to exchange information and coordinate population behaviors when their numbers reach a certain density, regulating gene expression. When activated, bacteria secrete signal molecules that can freely diffuse across the cell membrane. Once the concentration of these signal molecules in the environment reaches a certain threshold, it induces the expression of specific genes in the bacteria. We introduced the LuxI/R system, where AHL (N-acyl homoserine lactone) is produced catalytically by the expression of the LuxI gene as a signaling molecule. This AHL freely diffuses into the second engineered bacteria and binds with the LuxR protein released by the activation of the LuxR gene, activating the P LuxR promoter to initiate downstream gene expression."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/description/biocircuit-p1.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/description/biocircuit-p2.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/description/biocircuit-p3.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/description/biocircuit-p4.png"},"children":[]}]},{"type":"element","tag":"h3","props":{"id":"hardware-1"},"children":[{"type":"text","value":"Hardware"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"In response to the demands for standardized cultivation of engineered bacteria and high-sensitivity detection, we have designed a highly integrated and automated system that encompasses the entire process of bacterial strain revival, cultivation, detection, and analysis. The system utilizes an ESP32 chip as the main controller, interfaces with a temperature control system and a fluorescence detection system, employs a linear slide rail structure for high-throughput detection, and leverages a mobile app for automated device control and visualization of detection results. This system overcomes the limitations of time and space associated with traditional detection equipment, providing a versatile, portable, and highly sensitive solution for the detection of EDCs in aquatic environments across various scenarios."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/description/and-gate.png"},"children":[]}]},{"type":"element","tag":"h2","props":{"id":"reference"},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"text","value":"Reference"}]}]},{"type":"element","tag":"mdc-wrap","props":{":class":"reference"},"children":[{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Cao Y, Li L, Shen KH, Liu JG. Disease burden attributable to endocrine-disrupting chemicals exposure in China: A case study of phthalates. Science of the Total Environment, 2019, Vol. 662(1): 615-621"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Nematollah Asadi, Mahmoud Bahmani, Arash Kheradmand, Mahmoud Rafieian-Kopaei. The Impact of Oxidative Stress on Testicular Function and the Role of Antioxidants in Improving it: A Review. Journal of Clinical and Diagnostic Research, 2017, Vol. 11(5): IE1-IE5"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Ma Ya, Liu Haohao, Wu Jinxia, Yuan Le, Wang Yueqin, Du Xingde, Wang Rui, Marwa Phelisters Wegesa, Petlulu Pavankumar, Chen Xinghai, Zhang Huizhen. The adverse health effects of bisphenol A and related toxicity mechanisms. Environmental Research, 2019, Vol. 176: 108"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Liang R , Zhou J , Liu J. Construction of a Bacterial Assay for Estrogen Detection Based on an Estrogen-Sensitive Intein. Applied & Environmental Microbiology, 2011, 77(7):2488.DOI:10.1128/AEM.02336-10."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Ma Ya, Liu Haohao, Wu Jinxia, Yuan Le, Wang Yueqin, Du Xingde, Wang Rui, Marwa, Phelisters Wegesa, Petlulu, Pavankumar, Chen Xinghai, Zhang Huizhen.The adverse health effects of bisphenol A and related toxicity mechanisms.Environmental Research, 2019, Vol.176: 108575"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"LIANG Jing-jia, GU Ai-hua. Multigenerational and cross-generational effect of environmental endocrine disruptors on reproductive system in male animals. "},{"type":"element","tag":"em","props":{},"children":[{"type":"text","value":"Chinese Journal of Public Health"}]},{"type":"text","value":", 2021, 37(2): 375-380."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Nematollah Asadi, Mahmoud Bahmani, Arash Kheradmand, Mahmoud Rafieian-Kopaei.The Impact of Oxidative Stress on Testicular Function and the Role of Antioxidants in Improving it: A Review.Journal of Clinical and Diagnostic Research, 2017, Vol.11(5): IE1-IE5"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Mondal Shirsha, Ghosh Songita, Bhattacharya Samir, Mukherjee Sutapa. Chronic dietary administration of lower levels of diethyl phthalate induces murine testicular germ cell inflammation and sperm pathologies: Involvement of oxidative stress. Chemosphere, 2019, Vol.229: 443-451"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Maslowska K H, Karolina Makiela‐Dzbenska, Fijalkowska I J. The SOS system: A complex and tightly regulated response to DNA damage. John Wiley & Sons, Ltd, 2019(4). DOI:10.1002/em.22267."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Baojun W, Mauricio B, Martin B. Engineering modular and tunable genetic amplifiers for scaling transcriptional signals in cascaded gene networks. Nucleic Acids Research, 2014(14): 9484-9492. DOI:10.1093/nar/gku593."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Xiao Y, Zou H, Li J, Song T, Lv W, Wang W, Wang Z, Tao S. Impact of quorum sensing signaling molecules in gram-negative bacteria on host cells: current understanding and future perspectives. Gut Microbes. 2022 Jan-Dec, 14(1):2039048. doi: 10.1080/19490976.2022.2039048."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Drepper T, Eggert T, Circolone F, et al. Reporter proteins for in vivo fluorescence without oxygen. Nature Biotechnology, 2007, 25(4):443-445.DOI:10.1038/nbt1293."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Mukherjee A, Schroeder C M, Sikes H D, et al. Flavin-based fluorescent proteins: emerging paradigms in biological imaging. "},{"type":"element","tag":"em","props":{},"children":[{"type":"text","value":"This review comes from a themed issue on Analytical biotechnology"}]},{"type":"text","value":". 2015."}]}]}]}],"toc":{"title":"","searchDepth":1,"depth":3,"links":[{"id":"background","depth":2,"text":"Background"},{"id":"problem","depth":2,"text":"Problem"},{"id":"inspiration","depth":2,"text":"Inspiration","children":[{"id":"biology","depth":3,"text":"biology"},{"id":"hardware","depth":3,"text":"Hardware"}]},{"id":"solution","depth":2,"text":"Solution","children":[{"id":"biology-1","depth":3,"text":"biology"},{"id":"hardware-1","depth":3,"text":"Hardware"}]},{"id":"reference","depth":2,"text":"Reference"}]}},"_type":"markdown","_id":"content:project-description.md","_source":"content","_file":"project-description.md","_stem":"project-description","_extension":"md"},{"_path":"/project-engineering","_dir":"","_draft":false,"_partial":false,"_locale":"","title":"Project Engineering","description":"","body":{"type":"root","children":[{"type":"element","tag":"h2","props":{"id":"biosensor"},"children":[{"type":"text","value":"Biosensor"}]},{"type":"element","tag":"h3","props":{"id":"luminescence"},"children":[{"type":"text","value":"Luminescence"}]},{"type":"element","tag":"h4","props":{"id":"circle-design-reca-bsfbfp"},"children":[{"type":"text","value":"Circle: Design recA-BsFbFp"}]},{"type":"element","tag":"h5","props":{"id":"design"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In order to adapt to the low oxygen environment of the hardware design, we replaced the egfp gene, which strictly requires oxygen molecules as a cofactor for its luminescence, with the reporter gene BsFbFp. This gene can effectively express fluorescent signals in low or anaerobic environments."}]},{"type":"element","tag":"h5","props":{"id":"build"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"BsFbFp circuits","src":"https://static.igem.wiki/teams/5358/engineering-success/bsfbfp-circuits.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 1. BsFbFp circuits"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Genetic circuit: Gene recA and BsFbFp were all synthesized by Sangon Biotech according to BIOBRICK standard, and the gene circuit was obtained by PCR and verified by agarose gel electrophoresis."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"build results of BsFbFp ","src":"https://static.igem.wiki/teams/5358/engineering-success/build-results-of-bsfbfp.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 2. Build results of BsFbfP"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Construction of plasmid: The results of the agarose gel electrophoresis successfully showed bands for recA, the vector and BsFbFp, confirming that the gene circuit was successfully connected to the vector."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Ideal Strain: The successfully constructed plasmid was transformed into BL21 competent cells and cultured on LB solid medium with ampicillin."}]},{"type":"element","tag":"h5","props":{"id":"test"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Adding 600μL of bacterial solution and 30μL of ampicillin to 30mL of fresh culture medium, logarithmic growth was performed. Then, NA (Nalidixic Acid) was added in a gradient based on concentration to induce the bacteria. Subsequently, 250μL from each group was added to a anaerobic chamber and incubated on a shaking incubator at 37°C with a speed of 180 r/min. After 2 hours, the OD value and fluorescence intensity were measured."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"RFU-BsFbFp in 10lianpaichip(anaerobic chamber)","src":"https://static.igem.wiki/teams/5358/engineering-success/rfu-bsfbfp-in-10lianpaichip-anaerobic-chamber.jpg"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 3. RFU-BsFbFp in 10lianpaichip(anaerobic chamber)"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​The RFU (Relative Fluorescence Units) of the three groups of engineered bacteria increased linearly with the amount of NA (Nalidixic Acid) added. The results indicate that the fluorescent expression circuit of BsFbFp has been successfully constructed, and the engineered bacteria can effectively express fluorescence in an anaerobic environment."}]},{"type":"element","tag":"h5","props":{"id":"learn"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In conclusion，the plasmid constructed using BsFbFp as a reporter gene can effectively express fluorescence in low-oxygen or anaerobic environments, which is beneficial for the design of the hardware components."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​So we believed that the work in this cycle has been successfully accomplished., and we obtained the engineered bacteria needed for our goals."}]},{"type":"element","tag":"h3","props":{"id":"and-gate-design"},"children":[{"type":"text","value":"AND Gate Design"}]},{"type":"element","tag":"h4","props":{"id":"circle-1-design-pet28-reca-hrp-egfp"},"children":[{"type":"text","value":"Circle 1: Design pET28-recA-HRP-egfp"}]},{"type":"element","tag":"h5","props":{"id":"design-1"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​To construct the and gate, we introduced the hrp system, composed of the hrpR gene and hrpS gene, as well as the HrpL promoter regulated by the sigma54 factor. The hrpL promoter will only be activated when both the hrpR gene and the hrpS gene are expressed simultaneously."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In 2023, the BIT team successfully constructed P recA, the Hrp system, and egfp on the pUC19 plasmid. However, tests showed that the expression level of the circuit constructed using the pUC19 plasmid as a vector was relatively low, significantly deviating from the expected results. Therefore, we plan to switch to the pET28 plasmid, which has a more prominent expression level, to facilitate the synergistic assessment of endocrine disruption effects and oxidative damage effects."}]},{"type":"element","tag":"h5","props":{"id":"build-1"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Genetic circuit: recA-HRP-egfp were synthesized by Sangon Biotech according to BIOBRICK standard， the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir1-1","src":"https://static.igem.wiki/teams/5358/engineering-success/cir1-1.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 4. enzyme digestion result of circle 1"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nThe results of the agarose gel electrophoresis indicated that the vector was successfully digested and the target fragment was successfully amplified by PCR. The ligation was carried out at a 1:4 ratio of vector to target fragment, followed by transformation into DH5α competent cells and verification through agarose gel electrophoresis."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir1-2","src":"https://static.igem.wiki/teams/5358/engineering-success/cir1-2.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 5. build result of circle 1"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nVerification results: In multiple rounds of construction validation experiments, the target fragment showed no band in the agarose gel electrophoresis results, and the restriction enzyme digestion verification indicated self-ligation of the vector, proving that the construction in this cycle has failed."}]},{"type":"element","tag":"h5","props":{"id":"test-1"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Due to the failure of the target plasmid construction in this cycle, it is necessary to analyze the issues present in the plasmid design and construction process and make corrections."}]},{"type":"element","tag":"h5","props":{"id":"learn-1"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​During this construction process, we overlooked the reagent concentration ratio of the vector to the target fragment in our ligation system, which may be a major factor contributing to the failure of ligation. We plan to introduce the concentration factor of the ligation reagents into the ratio to improve the efficiency of ligation."}]},{"type":"element","tag":"h4","props":{"id":"circle-2-adjust-the-ligation-system-and-reconstruct-the-plasmid-pet28-reca-hrp-egfp"},"children":[{"type":"text","value":"Circle 2: Adjust the ligation system and reconstruct the plasmid pET28-recA-HRP-egfp."}]},{"type":"element","tag":"h5","props":{"id":"design-2"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Based on the experience learned from Circle 1, we plan to use a new ligation system, increase the dosage of the target fragment, and improve the ligation efficiency."}]},{"type":"element","tag":"h5","props":{"id":"build-2"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​The construction process used three different ligation systems, with the ratios of vector to target fragment being 1:4, 1:5, and 1:6, respectively. The constructed plasmids were extracted and verified by PCR followed by agarose gel electrophoresis."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir2","src":"https://static.igem.wiki/teams/5358/engineering-success/cir2.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 6. built result of circle 2"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nConstruction of plasmid: In the multi-round adjustment experiments of the ligation system construction, the agarose gel electrophoresis results still did not show a band for the target fragment, indicating that the construction in this cycle was unsuccessful."}]},{"type":"element","tag":"h5","props":{"id":"test-2"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Due to the failure of target plasmid construction in this cycle, testing cannot be carried out."}]},{"type":"element","tag":"h5","props":{"id":"learn-2"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In this cycle, we have corrected the ligation system, removing this influencing factor. Therefore, we suspect that the low partial digestion efficiency at the target sequence enzyme cutting sites may be the cause. This could be due to issues with the setting of protective bases near the enzyme cutting sites when designing the PCR primers, leading to another ligation failure. As such, we plan to redesign the PCR primers and increase the number of protective bases on both sides of the enzyme cutting sites from one or none to three to improve the success rate of the ligation."}]},{"type":"element","tag":"h4","props":{"id":"circle-3-redesign-the-primers-increase-protective-bases-and-reconstruct-pet28-reca-hrp-egfp"},"children":[{"type":"text","value":"Circle 3: Redesign the primers, increase protective bases, and reconstruct pET28-recA-HRP-egfp"}]},{"type":"element","tag":"h5","props":{"id":"design-3"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Based on the experience learned from Circle 2, we have redesigned the PCR primers for the target fragment and added protective bases to them, and we are reconstructing the pET28-recA-HRP-egfp plasmid."}]},{"type":"element","tag":"h5","props":{"id":"build-3"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​After changing to new PCR primers and setting the protective bases on both sides of the restriction enzyme cutting sites to 3, the construction was carried out. Genetic circuits: recA-HRP-egfp were synthesized by Sangon Biotech according the BIOBRICK standard, the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir3","src":"https://static.igem.wiki/teams/5358/engineering-success/cir3.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 7. built result of circle 3"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nThe agarose gel electrophoresis results showed that after redesigning the PCR primers and increasing the number of protective bases on both sides of the restriction enzyme cutting sites from one or none to three, only one band was observed for the target fragment, indicating that the new PCR primers have strong specificity and the target fragment was successfully obtained.\n​\nHowever, due to time constraints, we did not proceed with the subsequent experiments."}]},{"type":"element","tag":"h5","props":{"id":"test-3"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Limited by time, we were unable to complete the reconstruction, and therefore have no test results."}]},{"type":"element","tag":"h5","props":{"id":"learn-3"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In this cycle, the new PCR primers and the addition of protective bases at both ends of the restriction enzyme sites are beneficial for improving the success rate of enzyme ligation, thereby providing an experimental basis for the successful construction of the gate circuit."}]},{"type":"element","tag":"h2","props":{"id":"hardware"},"children":[{"type":"text","value":"Hardware"}]},{"type":"element","tag":"h4","props":{"id":"objectives"},"children":[{"type":"text","value":"Objectives"}]},{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To provide a high-throughput portable reaction vessel."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To provide stable physical conditions for detection."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"High-sensitivity fluorescence detection."}]}]},{"type":"element","tag":"h4","props":{"id":"design-4"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Our entire device mainly consists of three major parts: the outer casing that encases the entire device, the reaction area located in the upper layer, and the detection area situated in the lower layer. In the initial design, we utilized an STM32 chip as the main controller to regulate temperature control, fluorescence detection, and Bluetooth communication functions. In the second iteration, we replaced the main controller with an ESP32 chip that integrates a wireless communication module, and we adopted integrated temperature control and detection modules, which further enhances the system's stability and accuracy. To meet the need for simultaneous detection of multiple samples, we also redesigned the high-throughput reaction chambers, achieving rapid successive detection through a linear slide rail system."}]},{"type":"element","tag":"table","props":{},"children":[{"type":"element","tag":"thead","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"th","props":{},"children":[]},{"type":"element","tag":"th","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Initial Scheme"}]}]}]},{"type":"element","tag":"th","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Final Scheme"}]}]}]},{"type":"element","tag":"th","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Advantages After Improvement"}]}]}]}]}]},{"type":"element","tag":"tbody","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"MCU"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"STM32F103C8T6"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Arduino Nano ESP32"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Integrate Bluetooth communication module, reduce the complexity of the design"}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Temperature Control"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"TB6612FNG"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"TCM1040"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"More precise control of temperature"}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Fluorescence Detection"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"AS7341"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"FM-4A"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Integrate excitation light and fluorescent sensor to make the detection result more stable."}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Reaction Platform"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Single Reaction Tube"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Slide Rail Platform"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Achieve high-throughput detection"}]}]}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["tab-title"]},"children":[{"type":"text","value":"Table 1. Scheme difference"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nIn terms of material selection, we have adopted a black silicone kit and an aluminum alloy temperature-controlled slot as the environment for the centrifuge tubes. This scheme can excellently meet the insulation requirements of the design."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Edition Scheme Process","src":"https://static.igem.wiki/teams/5358/engineering-success/edition-scheme-process.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 8. Edition Scheme Process"}]}]},{"type":"element","tag":"h4","props":{"id":"establishment"},"children":[{"type":"text","value":"Establishment"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​The system construction utilized common integrated modules, with the main controller being an ESP32 chip, and temperature control and detection were accomplished using the TCM temperature control module and LIDE Technology's FM series digital fluorescence detection module, respectively. We employed industrial processing techniques to fabricate the aluminum alloy temperature-controlled slots, while the casing and other components were created using 3D printing technology."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Schematic Diagram of Hardware Equipment","src":"https://static.igem.wiki/teams/5358/engineering-success/new.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 9. Schematic Diagram of Hardware Equipment"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Model Diagram of Hardware Equipment","src":"https://static.igem.wiki/teams/5358/engineering-success/model-diagram-of-hardware-equipment.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 10. Model Diagram of Hardware Equipment"}]}]},{"type":"element","tag":"h4","props":{"id":"test-4"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​After finalizing the initial plan, we first carried out feasibility tests to verify the functionality of the fluorescence system and the temperature control system. After assembling the initial version of the model, numerous issues were identified through usage testing and preliminary risk analysis, such as the susceptibility of the fluorescence system to interference and the instability of the temperature control system. Through a cycle of testing, feedback, improvement, and retesting, the final version of the model demonstrated excellent performance across all aspects. Experiments showed that fluorescence identification was highly accurate, and the overall aesthetics were significantly enhanced."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Results of Temperature Control Experiment","src":"https://static.igem.wiki/teams/5358/engineering-success/results-of-temperature-control-experiment.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 11. Results of Temperature Control Experiment"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Results of Flurescence Verification Experiment","src":"https://static.igem.wiki/teams/5358/engineering-success/results-of-flurescence-verification-experiment.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 12. Results of Flurescence Verification Experiment"}]}]},{"type":"element","tag":"h4","props":{"id":"learn-4"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​During the process of designing the device, we encountered various challenges, such as overly complex control systems, insufficient stability, inability to conduct multi-sample testing, and issues with the optical path design. Throughout the real-time testing and discussion phases, we continuously documented various issues, proposed improvement plans, and actively sought help from knowledgeable teachers and consultants. Eventually, we completed a hardware design that met the requirements. Through continuous learning, improvement, and questioning, our expertise in the relevant fields has been greatly boosted."}]},{"type":"element","tag":"h2","props":{"id":"software"},"children":[{"type":"text","value":"Software"}]},{"type":"element","tag":"h4","props":{"id":"objectives-1"},"children":[{"type":"text","value":"Objectives"}]},{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To implement human-machine interaction for information exchange with hardware."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To automatically process data sent by the hardware."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To visualize data."}]}]},{"type":"element","tag":"h4","props":{"id":"design-5"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​The main body is divided into two 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discoveryAgent.finished TO this.onDeviceConnected\n​\n// Start searching for Bluetooth devices\nCALL discoveryAgent.start()\n​\n// Initialize Bluetooth Socket\nCREATE socket AS new BluetoothSocket USING RfcommProtocol\n​\n// Connect button click signal to send STARTSTART command\nCONNECT ui.pushButton.clicked TO this.sendStartSignal\n"}]}]},{"type":"element","tag":"h4","props":{"id":"plotting-module-performs-data-visualization"},"children":[{"type":"text","value":"Plotting module performs data visualization"}]},{"type":"element","tag":"pre","props":{"code":"FUNCTION updateGraphs()\n  // Create canvas\n  CREATE new scene\n  SET scene to graphicsView\n​\n  // Draw temperature graph\n  FOR i FROM 1 TO size of temperatureData - 1 DO\n    ADD line FROM (i-1, temperatureData[i-1]) TO (i, temperatureData[i]) WITH color red\n​\n  // Draw fluorescence intensity graph\n  FOR i FROM 1 TO size of fluorescenceData - 1 DO\n    ADD line FROM (i-1, fluorescenceData[i-1]) TO (i, fluorescenceData[i]) WITH color blue\n​\n  UPDATE graphicsView\nEND FUNCTION\n"},"children":[{"type":"element","tag":"code","props":{"__ignoreMap":""},"children":[{"type":"text","value":"FUNCTION updateGraphs()\n  // Create canvas\n  CREATE new scene\n  SET scene to graphicsView\n​\n  // Draw temperature graph\n  FOR i FROM 1 TO size of temperatureData - 1 DO\n    ADD line FROM (i-1, temperatureData[i-1]) TO (i, temperatureData[i]) WITH color red\n​\n  // Draw fluorescence intensity graph\n  FOR i FROM 1 TO size of fluorescenceData - 1 DO\n    ADD line FROM (i-1, fluorescenceData[i-1]) TO (i, fluorescenceData[i]) WITH color blue\n​\n  UPDATE graphicsView\nEND FUNCTION\n"}]}]},{"type":"element","tag":"h4","props":{"id":"data-segmentation-module"},"children":[{"type":"text","value":"Data segmentation module"}]},{"type":"element","tag":"pre","props":{"code":"FUNCTION splitAndFindMax(dataPoints, zeroThresholdCount)\n    INITIALIZE maxValues AS empty list\n    INITIALIZE currentSegment AS empty list\n    SET zeroCount TO 0\n​\n    FOR each point IN dataPoints DO\n        IF point.value == 0.0 THEN\n            INCREMENT zeroCount\n            IF zeroCount >= zeroThresholdCount THEN\n                IF currentSegment is not empty THEN\n                    SET maxVal TO maximum value in currentSegment\n                    ADD maxVal TO maxValues\n                    CLEAR currentSegment\n                    SET zeroCount TO 0  // Reset the counter​\n                CONTINUE  // Skip the current point, do not include in currentSegment​\n            END IF\n        ELSE\n            SET zeroCount TO 0  // Reset the counter because a non-zero value was encountered\n        END IF\n​\n        //Add the current point to the currentSegment\n        ADD point TO currentSegment\n    END FOR\n​\n    // Process the last segment\n    IF currentSegment is not empty THEN\n        SET maxVal TO maximum value in currentSegment\n        ADD maxVal TO maxValues\n    END IF\n    RETURN maxValues\nEND FUNCTION\n"},"children":[{"type":"element","tag":"code","props":{"__ignoreMap":""},"children":[{"type":"text","value":"FUNCTION splitAndFindMax(dataPoints, zeroThresholdCount)\n    INITIALIZE maxValues AS empty list\n    INITIALIZE currentSegment AS empty list\n    SET zeroCount TO 0\n​\n    FOR each point IN dataPoints DO\n        IF point.value == 0.0 THEN\n            INCREMENT zeroCount\n            IF zeroCount >= zeroThresholdCount THEN\n                IF currentSegment is not empty THEN\n                    SET maxVal TO maximum value in currentSegment\n                    ADD maxVal TO maxValues\n                    CLEAR currentSegment\n                    SET zeroCount TO 0  // Reset the counter​\n                CONTINUE  // Skip the current point, do not include in currentSegment​\n            END IF\n        ELSE\n            SET zeroCount TO 0  // Reset the counter because a non-zero value was encountered\n        END IF\n​\n        //Add the current point to the currentSegment\n        ADD point TO currentSegment\n    END FOR\n​\n    // Process the last segment\n    IF currentSegment is not empty THEN\n        SET maxVal TO maximum value in currentSegment\n        ADD maxVal TO maxValues\n    END IF\n    RETURN maxValues\nEND FUNCTION\n"}]}]},{"type":"element","tag":"h4","props":{"id":"testing"},"children":[{"type":"text","value":"Testing"}]}],"toc":{"title":"","searchDepth":1,"depth":3,"links":[{"id":"biosensor","depth":2,"text":"Biosensor","children":[{"id":"luminescence","depth":3,"text":"Luminescence","children":[{"id":"circle-design-reca-bsfbfp","depth":4,"text":"Circle: Design recA-BsFbFp"}]},{"id":"and-gate-design","depth":3,"text":"AND Gate Design","children":[{"id":"circle-1-design-pet28-reca-hrp-egfp","depth":4,"text":"Circle 1: Design pET28-recA-HRP-egfp"},{"id":"circle-2-adjust-the-ligation-system-and-reconstruct-the-plasmid-pet28-reca-hrp-egfp","depth":4,"text":"Circle 2: Adjust the ligation system and reconstruct the plasmid pET28-recA-HRP-egfp."},{"id":"circle-3-redesign-the-primers-increase-protective-bases-and-reconstruct-pet28-reca-hrp-egfp","depth":4,"text":"Circle 3: Redesign the primers, increase protective bases, and reconstruct pET28-recA-HRP-egfp"}]}]},{"id":"hardware","depth":2,"text":"Hardware","children":[{"id":"objectives","depth":4,"text":"Objectives"},{"id":"design-4","depth":4,"text":"Design"},{"id":"establishment","depth":4,"text":"Establishment"},{"id":"test-4","depth":4,"text":"Test"},{"id":"learn-4","depth":4,"text":"Learn"}]},{"id":"software","depth":2,"text":"Software","children":[{"id":"objectives-1","depth":4,"text":"Objectives"},{"id":"design-5","depth":4,"text":"Design"},{"id":"bluetooth-module-receives-data","depth":4,"text":"Bluetooth module receives data"},{"id":"plotting-module-performs-data-visualization","depth":4,"text":"Plotting module performs data visualization"},{"id":"data-segmentation-module","depth":4,"text":"Data segmentation module"},{"id":"testing","depth":4,"text":"Testing"}]}]}},"_type":"markdown","_id":"content:project-engineering.md","_source":"content","_file":"project-engineering.md","_stem":"project-engineering","_extension":"md"},{"_path":"/project-safety","_dir":"","_draft":false,"_partial":false,"_locale":"","title":"Safety","description":"Synthetic biology has the ability to change the world in amazing ways. From treating cancer to alleviating the climate crisis, we do have the ability to build a better world at iGEM. However, by working with some unpredictable organisms, we must also understand that the good works we create can actually cause harm. When looking at the big picture, we must also understand that we must be carefully every day. As researchers, we need to manage the community and environmental risks.","body":{"type":"root","children":[{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Synthetic biology has the ability to change the world in amazing ways. From treating cancer to alleviating the climate crisis, we do have the ability to build a better world at iGEM. However, by working with some unpredictable organisms, we must also understand that the good works we create can actually cause harm. When looking at the big picture, we must also understand that we must be carefully every day. As researchers, we need to manage the community and environmental risks."}]},{"type":"element","tag":"h2","props":{"id":"the-rules-and-policies-of-the-igem"},"children":[{"type":"text","value":"The rules and policies of the iGEM"}]},{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Release Beyond Containment: You cannot release or deploy any genetically modified organisms outside the lab. You must Check In before bringing any product of synthetic biology outside of the lab."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"White List: You must Check In before beginning experiments with any organism, part, or activity that is not on the White List."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"No Human Experimentation: Testing your project on humans (including yourselves) is strictly prohibited. You must check in before beginning any laboratory experiments involving humans or human samples (including but not limited to blood, DNA, other bodily specimens, and health or psychological outcomes)."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Animal Use: You must Check In and submit an Animal Use Form before beginning any experiments involving animals or animal samples. This includes vertebrates (e.g. rats, mice, guinea pigs, hamsters, amphibians, and fishes) and higher order invertebrates(e.g. cuttlefish, octopus, squid, lobster, bees, or American or German cockroaches)."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Human Subjects Research: All human subjects research (e.g.surveys, interviews, public engagement) you carry out must be done in accordance with relevant laws and regulations, as well as any institutional rules or guidance."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Antimicrobial Resistance: You must Check In before beginning experiments likely to increase the risk posed by antimicrobial resistance (e.g. by using novel resistance factors, adding known factors into new organisms, or resistance factors not previously used in your facility)."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Gene Drive: Gene Drive is not allowed in iGEM projects without a special exception from the Safety & Security Committee."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Coronavirus: Any work using live SARS-CoV-2 virus(the causative agent of COVID-19) must follow World Health Organization (WHO) guidance, in addition to all local and institutional rules. WHO guidance may be stricter than local safety rules. You must Check In before beginning experiments with live SARS-CoV-2 or parts from SARS-CoV-2."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Environmental Samples: As long as parts or organisms are not isolated from them, samples from the environment, the food industry, or other non- traditional suppliers may be used in your experiments You must Check In before using any parts organisms obtained from outside the lab or from non- traditional suppliers."}]}]},{"type":"element","tag":"h2","props":{"id":"project-safety"},"children":[{"type":"text","value":"Project safety"}]},{"type":"element","tag":"h3","props":{"id":"safety-of-project-design"},"children":[{"type":"text","value":"Safety of project design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Our project employs the insertion of RecA intein into T7 RNAP to detect endocrine disruption effects and utilizes the SOS response mechanism of Escherichia coli to detect DNA damage. 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The core goal is to develop a highly sensitive microbial sensor. The principle of the sensor's response to DNA damage is based on the intein's cleavage and the bacterial SOS response, which initiates downstream transcriptional activity and expresses fluorescent proteins. We will collaboratively develop portable detection hardware to achieve efficient and portable detection outdoors. The hardware will provide an approximately anaerobic environment to ensure stable characterization under anoxic conditions. 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An aluminum alloy temperature-controlled slot houses multiple reaction tubes, with temperature control achieved through a temperature control module."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"l Detection Area: A linear slide rail drives the fluorescence detector to sequentially inspect each reaction tube, enabling high-throughput detection."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The functions of each module are centrally controlled using an MCU chip."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/contribution/hardware-fig2.png"},"children":[]}]},{"type":"element","tag":"div","props":{"style":"text-align: center;"},"children":[{"type":"text","value":"\nFigure 2 Hardware Structure Diagram\n"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The main components selected for the 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This damage can occur through various mechanisms and can lead to a range of cellular consequences if not properly repaired."}]},{"type":"element","tag":"h2","props":{"id":"experiment"},"children":[{"type":"text","value":"Experiment"}]},{"type":"element","tag":"h3","props":{"id":"strain-obtain"},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Strain Obtain"}]}]},{"type":"element","tag":"h4","props":{"id":"gene-acquisition"},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"gene acquisition"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The recA，rbs30，hrpR，hrpS，PhrpL ，eGFP，BsFbFp，PgapA，T7RNAP，VAMER intein，PT7，LuxI and LuxR genes were synthesized by Sangon Biotech according to the standard of biobrick, and the line fragments were obtained through PCR."}]},{"type":"element","tag":"h5","props":{"id":"pcr"},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"PCR"}]}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Material"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Thermocycler, (0.2ml)PCR tube, pipetting gun, pipette tip, 2xPCRmix, upstream primer, downstream primer, sterile water, DNA template."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Protocol"}]},{"type":"element","tag":"ul","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Add to PCR tube (the total system volume: 25 μL): 12.5 μL 2×PCRmix, 9 μL of sterile water, 1.5 μl DNA template, 1 μL reverse primer, 1 μL forward primer;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Put the PCR tube into the thermocycler and execute the program: pre-denaturation at 94°C for 210s; followed by 30 cycles of denaturation at 94°C for 30s, anneal at the range of 52-62°C for 30s, extension at 72°C for 60s;and a final extension at 72°C for 5 min;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"The product is stored at -10°C;"}]}]},{"type":"element","tag":"h5","props":{"id":"agarose-gel-electrophoresis-and-sample-recovery"},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Agarose gel electrophoresis and sample recovery"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Material"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Electric hot water bath, electronic balance, electrophoresis instrument, gel imaging analysis system, pipetting gun, pipette tip, glass bottle, agarose, electrophoresis buffer, nucleic acid dye, DNA loading buffer, DNA precipitation buffer, gel buffer, elution buffer;"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Protocol"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Agarose gel electrophoresis"}]}]},{"type":"element","tag":"ul","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"2% agarose solution: add 30mL of 1X TAE electrophoresis buffer into a glass bottle. then weigh 0.6g of agarose and add it into the bottle;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Heat the mixture(2% agarose solution) to boiling. Add 3μL of fluorescent dye to the heated liquid and mix it completely. Take out the glue box and place a mold in the box. Then pour the mixture into the mold and wait for solidification at room temperature;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Remove the cooled and formed agarose gel and place it in the electrophoresis instrument. Mix the DNA samples with 1/5 of DNA precipitation buffer thoroughly. Then inject the standard samples and the mixture into different holes of the gel. Turn on the electrophoresis instrument and adjust the voltage to 110V for 40 min;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Take out the agarose gel and open the gel imaging analysis instrument to analyze and save the electrophoresis results."}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Sample recovery"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The electrophoresis products were purified by gel recovery method, and the purified materials were using the DNA gel recovery kit of Axygen . The operation steps are as follows :"}]},{"type":"element","tag":"ul","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Open the water bath in advance and set the temperature to 75°C(167°F);"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Use Agarose gel electrophoresis to verify the DNA fragment and observe in the gel imaging system. Cut the agarose gel containing the target band with a knife under the ultraviolet lamp, and record the weight of the 1.5 mL centrifuge tube in advance. After the centrifuge tube is loaded, weigh it again and calculate the gel weight. 100 mg is a gel volume equivalent to 100 μL;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Add 3 gel volume of gel buffer DE-A, mix well and put into 75°C water bath heating 6-8 min, mix it up and down every 2 min during water bath;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Add 0.5 DE-A volume of gel buffer DE-B, uniform mix it, and add 1 gel volume of isopropanol;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Purify the resulting products by centrifugation. Transfer the above solution to an adsorption column and place it in a 2mL centrifuge tube. Centrifuge at 12,000 rpm for 1 min, discard the waste liquid, and place the adsorption column back into the collection tube;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Add 500 μL buffer W1 to the adsorption column, centrifuge at 12,000 rpm for 30s, pour the waste liquid, and place the adsorption column back into the collection tube;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Add 700 μL buffer W2 to the adsorption column, centrifuge at 12,000 rpm for 30s, pour the waste liquid and place the adsorption column back into the collection tube, and repeat the operation once again;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Put the adsorption column in a new 1.5ml centrifuge tube, add 30μl Eluent in the center of the adsorption column membrane, and then centrifuge at the 12,000rpm for 1min after standing at room temperature, from which electrophoretic products were obtained;"}]}]},{"type":"element","tag":"h4","props":{"id":"plasmid-construction"},"children":[{"type":"text","value":"Plasmid Construction"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The obtained gene line fragment and pUC19 empty vector are digested and ligated by enzyme ligation."}]},{"type":"element","tag":"h5","props":{"id":"enzyme-digestion"},"children":[{"type":"text","value":"Enzyme digestion"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Material"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Incubator, (0.2ml)PCR tube, pipetting gun, pipette tip, restriction enzyme, NEB Buffer 3.1, target gene"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Protocol"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"After adding the following enzyme digestion system to a 0.2 mL centrifuge tube, incubate it in an incubator at 37°C for 2 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After incubation, plate 100μL of the transformed cells onto LB agar plates containing 100μg/mL ampicillin resistance. Invert the plates and incubate overnight at 37°C. Pick single colonies and inoculate them into 30mL of antibiotic-free LB broth. Add 30μL of ampicillin and incubate at 37°C with shaking at 180r/min for 12 h. Preserve the cultures in glycerol at -10°C;"}]},{"type":"element","tag":"h5","props":{"id":"plasmid-transformation"},"children":[{"type":"text","value":"Plasmid transformation"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Material"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Vertical clean bench, shaker, water bath, centrifuge, pipetting gun, pipette tip, solid culture dish, alcohol lamp, applicator, ampicillin, LB-free liquid medium, competent cells;"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Protocol"}]},{"type":"element","tag":"ul","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Fill the foam box with about 2/3 of the volume of ice, and take100μL of prepared competent cells. turn on the ultra-clean table UV lamp in advance for 15min for sterilization. After sterilization, light up the alcohol lamp in the ultra-clean table, and transfer 50μL competent cells into a new aseptic centrifuge tube with a pipette beside the alcohol lamp, and add 5μL plasmid solution into it."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"place the centrifuge tube containing the plasmid solution in the ice box for 30min, turn on the water bath in advance and set the temperature at 42°C, heat the centrifuge tube after the ice bath in the water bath at 42°C for 45s, and immediately remove the centrifuge tube and place it in the ice box for 2min to complete the transformation process;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Take out the packaged liquid medium without anti-LB, and add 500μL liquid medium into the centrifuge tube after heating. 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Measure OD600 and fluorescence intensity of bacterial solution by microplate reader;"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Method 2：The bacterial solution was sealed using paraffin oil and DNA damaging agents were added under the paraffin oil page and incubated for 2h in a 37 C incubator. 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Set the wavelength to 600 and measure the absorbance reading."}]},{"type":"element","tag":"h5","props":{"id":"measure-the-fluorescence-intensity"},"children":[{"type":"text","value":"Measure the Fluorescence Intensity"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Transfer 200μL of the bacteria solution to a 96-hole black microplate and place it in a microplate reader. Set the excitation wavelength to 488 nm, emission wavelength to 520 nm, and gain to 75. Measure the fluorescence intensity."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Each group of data was measured three times and averaged."}]}],"toc":{"title":"","searchDepth":1,"depth":3,"links":[{"id":"what-is-endocrine-pollutant","depth":2,"text":"What IS Endocrine Pollutant？"},{"id":"what-is-dna-damage","depth":2,"text":"What IS DNA Damage？"},{"id":"experiment","depth":2,"text":"Experiment","children":[{"id":"strain-obtain","depth":3,"text":"Strain Obtain","children":[{"id":"gene-acquisition","depth":4,"text":"gene acquisition"},{"id":"plasmid-construction","depth":4,"text":"Plasmid Construction"}]},{"id":"characterization-experiment","depth":3,"text":"Characterization experiment","children":[{"id":"preparation","depth":4,"text":"Preparation"},{"id":"experimental-characterization-of-the-anaerobic-environment","depth":4,"text":"Experimental characterization of the anaerobic environment"},{"id":"data-measurement","depth":4,"text":"Data measurement"}]}]}]}},"_type":"markdown","_id":"content:wetlab-experiment.md","_source":"content","_file":"wetlab-experiment.md","_stem":"wetlab-experiment","_extension":"md"},{"_path":"/wetlab-notebook","_dir":"","_draft":false,"_partial":false,"_locale":"","title":"Notebook","description":"","body":{"type":"root","children":[{"type":"element","tag":"h2","props":{"id":"may-june"},"children":[{"type":"text","value":"May-June"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The team members conducted a background survey on the effects of DNA damage and endocrine interference, and designed a specific logical gate line based on this. Our project employs the insertion of RecA intein into T7 RNAP to detect endocrine disruption effects and utilizes the SOS response mechanism of Escherichia coli to detect DNA damage. Additionally, the LuxI/R system is introduced to achieve an AND gate detection of both effects. Furthermore, the Hrp gene regulation system and BsFbFp fluorescent gene are combined to amplify the transcriptional signal and ensure effective signal expression under anaerobic conditions"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/notebook/ahl.png"},"children":[]}]},{"type":"element","tag":"h2","props":{"id":"june-september"},"children":[{"type":"text","value":"June-September"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The experimental verification of the constructed lines is roughly divided into four links."}]},{"type":"element","tag":"h3","props":{"id":"_1-construction-of-reca-bsfbfp"},"children":[{"type":"text","value":"1. Construction of recA-BsFbFp"}]},{"type":"element","tag":"mdc-wrap","props":{":class":"paren-ol"},"children":[{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Plasmid Mini-Preparation (Extraction of the pUC19 Plasmid)"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"PCR Amplification (Amplification of a RecA fragment of approximately 100bp)"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Enzymatic Digestion (Digestion of the plasmid containing BsFbFP with different restriction enzymes)"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Agarose Gel Electrophoresis (Detection of the target fragment for subsequent recovery)"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Gel Extraction (Recovery of the target fragment from the agarose gel)"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"DNA Ligase Mediated Joining of DNA Fragments (Ligation to construct the recombinant plasmid)"}]}]}]},{"type":"element","tag":"h3","props":{"id":"_2-construction-of-reca-hrp-egfp"},"children":[{"type":"text","value":"2. Construction of recA-HRP-egfp"}]},{"type":"element","tag":"mdc-wrap","props":{":class":"paren-ol"},"children":[{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Plasmid Mini-Preparation (Extraction of the pET28 plasmid)"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"PCR Amplification (Amplification of a RecA fragment of approximately 100bp)"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Enzymatic Digestion (Digestion of the plasmid and PCR product with restriction enzymes)"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Agarose Gel Electrophoresis (Detection of the target fragment for subsequent recovery)"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Gel Extraction (Recovery of the target fragment from the agarose gel)"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"DNA Ligase Mediated Joining of DNA Fragments  (Ligation of recA with pET28)"}]}]}]},{"type":"element","tag":"h3","props":{"id":"_3-construction-of-pj23100-luxi-and-pj23100-plux-luxr-egfp"},"children":[{"type":"text","value":"3. 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Intein-mediated sequencing and ligation"}]},{"type":"element","tag":"h4","props":{"id":"_623-626"},"children":[{"type":"text","value":"6.23-6.26"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The initial attempt at constructing recA-BsFbFp was made, with successful enzymatic digestion but no bacterial colonies grown on the solid medium after ligation and cultivation. 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These substances are widely found in plastics, pesticides, industrial chemicals, and daily consumer goods. They enter the human body through the food chain or through direct contact, interfering with hormonal balance, and may cause reproductive health problems, developmental disorders, metabolic disorders and even some types of cancer.","body":{"type":"root","children":[{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Endocrine disruptors are a class of chemicals that can interfere with the normal function of the endocrine system, and they may mimic or inhibit the effects of natural hormones, thereby affecting the physiological processes of the body. These substances are widely found in plastics, pesticides, industrial chemicals, and daily consumer goods. They enter the human body through the food chain or through direct contact, interfering with hormonal balance, and may cause reproductive health problems, developmental disorders, metabolic disorders and even some types of cancer."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"When endocrine disruptors affect hormone activity, they may also induce cellular oxidative stress because they increase the generation of reactive oxygen species (ROS), highly reactive chemicals that damage cellular structures, including proteins, lipids, and DNA. Long-term oxidative stress accelerates cellular aging and may also lead to male infertility and the development of multiple chronic diseases. Thus, there is a close link between exposure to endocrine disruptors and cellular oxidative damage, a potential promoter to multiple health problems. Therefore, it is necessary to detect the endocrine interference effects of endocrine disruptors and oxidative damage effects in the relevant location samples."}]},{"type":"element","tag":"h2","props":{"id":"_1-puc19-preca-bsfbfp-bl21"},"children":[{"type":"text","value":"1. pUC19-PrecA-BsFbFp-BL21"}]},{"type":"element","tag":"h3","props":{"id":"strain-construction"},"children":[{"type":"text","value":"Strain construction"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We plan to design a portable microbial sensor, in which the sensor chamber will be maintained in a nearly anoxic state. Under anoxic conditions, the fluorescence intensity of EGFP diminishes, and its detection limit decreases; therefore, traditional EGFP is not the optimal choice for our application. We have constructed the PrecA-BsFbFP circuit, where BsFbFP is an anoxic fluorescent protein that exhibits fluorescence intensity nearly equivalent to that of EGFP under anoxic conditions, while significantly improving the detection limit. Consequently, we will utilize BsFbFP as the expression product of our engineered strain for detection characterization."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We obtained the PrecA-BsFbFp circuit and the recA-eGFP (hereafter referred to as wild) circuit through PCR.The gene circuits were ligated to the pUC19 vector using enzyme ligation and transformed into BL21 competent cells . The transformed cells were spread onto LB solid medium supplemented with ampicillin. Single colonies that grew were selected for amplification and sent to Jinweizhi (Suzhou) Biochemistry Co. for sequencing, and the remaining bacteria were preserved in glycerol."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/fig1.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/bsfbfp.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 1. Results of the enzyme digestion verification experiment for pUC19-PrecA-BsFbFP-BL21."}]}]},{"type":"element","tag":"h3","props":{"id":"characterization-of-experiments"},"children":[{"type":"text","value":"Characterization of experiments"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Firstly, we performed screening of the pUC19-PrecA-BsFbFp-BL21. The transformed wild-type bacteria were cultured on a shaker until the OD value reached 0.2, followed by the addition of NA for induction for 2 h. In aerobic conditions, BsFbFp is significantly less characterized than EGFP, while the RFU is close, and BsFbFp has a high detection limit, indicating that BsFbFp is an excellent choice for characterization in anaerobic conditions."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/o2-fluo.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/o2-rfu.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 2. Characterization results of BsFbFP and EGFP under anoxic and NA gradient injury conditions."}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We have characterized the pUC19-PrecA-BsFbFp-BL21 under anaerobic conditions.The transformed wild-type bacteria were cultured on a shaker until the OD value reached 0.2, followed by the addition of NA for induction for 2 h.As the concentration of NA increased, the fluorescence intensity of engineering strains increased accordingly. Several experiments proved that the RFU value increased with the concentration of NA in the anaerobic environment."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/10-rfu.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/10-rfu-more.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 3. Fluorescence intensity characterization of BsFbFP under NA gradient injury conditions in 10-lianpao chip cultures, expressed as RFU."}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Later, we compared the characterization difference between DH5α strain and BL21 strain, and showed that the BL21 strain has higher sensitivity to injury, reflecting its advantage as a representative strain."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/o2-fluo-more.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 4. Fluorescence characterization gradients of DH5α and BL21 under aerobic and NA gradient injury conditions."}]}]},{"type":"element","tag":"h2","props":{"id":"_2-pet28-preca-hrpr-hrps-phrpl-bsfbfp-bl21"},"children":[{"type":"text","value":"2. pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The gene circuits were ligated to the pET28 vector using enzyme ligation and transformed into BL21 competent cells . The transformed cells were spread onto LB solid medium supplemented with ampicillin.Preserved strains were stored in glycerol."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/hrp.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/2-enzyme-digestion.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 5. Results of the enzyme digestion verification experiment for pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21."}]}]},{"type":"element","tag":"h2","props":{"id":"_3-puc19-pbad-n-t7rnap-er-lbd-intein-c-t7rnap-bsfbfp"},"children":[{"type":"text","value":"3. pUC19-PBAD-N-T7RNAP-ER-LBD intein-C-T7RNAP-BsFbFp"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The gene sequencing conducted by our company has successfully validated the synthesis of the intein of the biological circuit."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/fig3.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/gene-squencing.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 6. Gene sequencing results of the ER-LBD intein."}]}]},{"type":"element","tag":"h2","props":{"id":"_4-psb1c3-pj23100-luxi-bl21-and-psb1c3-plux-luxr-egfp-bl21"},"children":[{"type":"text","value":"4. psB1C3-Pj23100-LuxI-BL21 and psB1C3-Plux-LuxR-EGFP-BL21"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/fig4.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"We obtained by PCR the Pj233100, Plux-LuxR, LuxI, and EGFP and ligated it to the psB1C3 plasmid into E. coli BL21. The transformed cells were spread onto LB solid medium supplemented with chloramphenicol. Recombinants were used for characterization experiments and stored in glycerol."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/pcr.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 7. PCR products of the Pj23100-LuxI fragment and the Plux-LuxR-EGFP fragment."}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/lux-digestion.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 8. Enzyme digestion verification of psB1C3-Pj23100-LuxI-BL21 and Plux-LuxR-EGFP-BL21."}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"img","src":"https://static.igem.wiki/teams/5358/experiment/ligation.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 9. Enzyme Ligation Product of psB1C3-Pj23100-LuxI-BL21 and Plux-LuxR-EGFP-BL21."}]}]}],"toc":{"title":"","searchDepth":1,"depth":3,"links":[{"id":"_1-puc19-preca-bsfbfp-bl21","depth":2,"text":"1. pUC19-PrecA-BsFbFp-BL21","children":[{"id":"strain-construction","depth":3,"text":"Strain construction"},{"id":"characterization-of-experiments","depth":3,"text":"Characterization of experiments"}]},{"id":"_2-pet28-preca-hrpr-hrps-phrpl-bsfbfp-bl21","depth":2,"text":"2. pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21"},{"id":"_3-puc19-pbad-n-t7rnap-er-lbd-intein-c-t7rnap-bsfbfp","depth":2,"text":"3. pUC19-PBAD-N-T7RNAP-ER-LBD intein-C-T7RNAP-BsFbFp"},{"id":"_4-psb1c3-pj23100-luxi-bl21-and-psb1c3-plux-luxr-egfp-bl21","depth":2,"text":"4. psB1C3-Pj23100-LuxI-BL21 and psB1C3-Plux-LuxR-EGFP-BL21"}]}},"_type":"markdown","_id":"content:wetlab-results.md","_source":"content","_file":"wetlab-results.md","_stem":"wetlab-results","_extension":"md"}],"navigation":[{"title":"Hardware","_path":"/drylab-hardware"},{"title":"Integrated Human Practice","_path":"/hp-ihp"},{"title":"Description","_path":"/project-description"},{"title":"Project Engineering","_path":"/project-engineering"},{"title":"Safety","_path":"/project-safety"},{"title":"Team Contribution","_path":"/team-contribution"},{"title":"Experiment","_path":"/wetlab-experiment"},{"title":"Notebook","_path":"/wetlab-notebook"},{"title":"Parts","_path":"/wetlab-parts"},{"title":"Results","_path":"/wetlab-results"}]}
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-{"_path":"/project-engineering","_dir":"","_draft":false,"_partial":false,"_locale":"","title":"Project Engineering","description":"","body":{"type":"root","children":[{"type":"element","tag":"h2","props":{"id":"biosensor"},"children":[{"type":"text","value":"Biosensor"}]},{"type":"element","tag":"h3","props":{"id":"luminescence"},"children":[{"type":"text","value":"Luminescence"}]},{"type":"element","tag":"h4","props":{"id":"circle-design-reca-bsfbfp"},"children":[{"type":"text","value":"Circle: Design recA-BsFbFp"}]},{"type":"element","tag":"h5","props":{"id":"design"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In order to adapt to the low oxygen environment of the hardware design, we replaced the egfp gene, which strictly requires oxygen molecules as a cofactor for its luminescence, with the reporter gene BsFbFp. This gene can effectively express fluorescent signals in low or anaerobic environments."}]},{"type":"element","tag":"h5","props":{"id":"build"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"BsFbFp circuits","src":"https://static.igem.wiki/teams/5358/engineering-success/bsfbfp-circuits.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 1. BsFbFp circuits"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\tGenetic circuit: Gene recA and BsFbFp were all synthesized by Sangon Biotech according to BIOBRICK standard, and the gene circuit was obtained by PCR and verified by agarose gel electrophoresis."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"build results of BsFbFp ","src":"https://static.igem.wiki/teams/5358/engineering-success/build-results-of-bsfbfp.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 2. Build results of BsFbfP"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Construction of plasmid: The results of the agarose gel electrophoresis successfully showed bands for recA, the vector and BsFbFp, confirming that the gene circuit was successfully connected to the vector."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Ideal Strain: The successfully constructed plasmid was transformed into BL21 competent cells and cultured on LB solid medium with ampicillin."}]},{"type":"element","tag":"h5","props":{"id":"test"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Adding 600μL of bacterial solution and 30μL of ampicillin to 30mL of fresh culture medium, logarithmic growth was performed. Then, NA (Nalidixic Acid) was added in a gradient based on concentration to induce the bacteria. Subsequently, 250μL from each group was added to a anaerobic chamber and incubated on a shaking incubator at 37°C with a speed of 180 r/min. After 2 hours, the OD value and fluorescence intensity were measured."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"RFU-BsFbFp in 10lianpaichip(anaerobic chamber)","src":"https://static.igem.wiki/teams/5358/engineering-success/rfu-bsfbfp-in-10lianpaichip-anaerobic-chamber.jpg"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 3. RFU-BsFbFp in 10lianpaichip(anaerobic chamber)"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​The RFU (Relative Fluorescence Units) of the three groups of engineered bacteria increased linearly with the amount of NA (Nalidixic Acid) added. The results indicate that the fluorescent expression circuit of BsFbFp has been successfully constructed, and the engineered bacteria can effectively express fluorescence in an anaerobic environment."}]},{"type":"element","tag":"h5","props":{"id":"learn"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In conclusion，the plasmid constructed using BsFbFp as a reporter gene can effectively express fluorescence in low-oxygen or anaerobic environments, which is beneficial for the design of the hardware components."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​So we believed that the work in this cycle has been successfully accomplished., and we obtained the engineered bacteria needed for our goals."}]},{"type":"element","tag":"h3","props":{"id":"and-gate-design"},"children":[{"type":"text","value":"AND Gate Design"}]},{"type":"element","tag":"h4","props":{"id":"circle-1-design-pet28-reca-hrp-egfp"},"children":[{"type":"text","value":"Circle 1: Design pET28-recA-HRP-egfp"}]},{"type":"element","tag":"h5","props":{"id":"design-1"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​To construct the and gate, we introduced the hrp system, composed of the hrpR gene and hrpS gene, as well as the HrpL promoter regulated by the sigma54 factor. The hrpL promoter will only be activated when both the hrpR gene and the hrpS gene are expressed simultaneously."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In 2023, the BIT team successfully constructed P recA, the Hrp system, and egfp on the pUC19 plasmid. However, tests showed that the expression level of the circuit constructed using the pUC19 plasmid as a vector was relatively low, significantly deviating from the expected results. Therefore, we plan to switch to the pET28 plasmid, which has a more prominent expression level, to facilitate the synergistic assessment of endocrine disruption effects and oxidative damage effects."}]},{"type":"element","tag":"h5","props":{"id":"build-1"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Genetic circuit: recA-HRP-egfp were synthesized by Sangon Biotech according to BIOBRICK standard， the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir1-1","src":"https://static.igem.wiki/teams/5358/engineering-success/cir1-1.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 4. enzyme digestion result of circle 1"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nThe results of the agarose gel electrophoresis indicated that the vector was successfully digested and the target fragment was successfully amplified by PCR. The ligation was carried out at a 1:4 ratio of vector to target fragment, followed by transformation into DH5α competent cells and verification through agarose gel electrophoresis."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir1-2","src":"https://static.igem.wiki/teams/5358/engineering-success/cir1-2.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 5. build result of circle 1"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nVerification results: In multiple rounds of construction validation experiments, the target fragment showed no band in the agarose gel electrophoresis results, and the restriction enzyme digestion verification indicated self-ligation of the vector, proving that the construction in this cycle has failed."}]},{"type":"element","tag":"h5","props":{"id":"test-1"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Due to the failure of the target plasmid construction in this cycle, it is necessary to analyze the issues present in the plasmid design and construction process and make corrections."}]},{"type":"element","tag":"h5","props":{"id":"learn-1"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​During this construction process, we overlooked the reagent concentration ratio of the vector to the target fragment in our ligation system, which may be a major factor contributing to the failure of ligation. We plan to introduce the concentration factor of the ligation reagents into the ratio to improve the efficiency of ligation."}]},{"type":"element","tag":"h4","props":{"id":"circle-2-adjust-the-ligation-system-and-reconstruct-the-plasmid-pet28-reca-hrp-egfp"},"children":[{"type":"text","value":"Circle 2: Adjust the ligation system and reconstruct the plasmid pET28-recA-HRP-egfp."}]},{"type":"element","tag":"h5","props":{"id":"design-2"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Based on the experience learned from Circle 1, we plan to use a new ligation system, increase the dosage of the target fragment, and improve the ligation efficiency."}]},{"type":"element","tag":"h5","props":{"id":"build-2"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​The construction process used three different ligation systems, with the ratios of vector to target fragment being 1:4, 1:5, and 1:6, respectively. The constructed plasmids were extracted and verified by PCR followed by agarose gel electrophoresis."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir2","src":"https://static.igem.wiki/teams/5358/engineering-success/cir2.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 6. built result of circle 2"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nConstruction of plasmid: In the multi-round adjustment experiments of the ligation system construction, the agarose gel electrophoresis results still did not show a band for the target fragment, indicating that the construction in this cycle was unsuccessful."}]},{"type":"element","tag":"h5","props":{"id":"test-2"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Due to the failure of target plasmid construction in this cycle, testing cannot be carried out."}]},{"type":"element","tag":"h5","props":{"id":"learn-2"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In this cycle, we have corrected the ligation system, removing this influencing factor. Therefore, we suspect that the low partial digestion efficiency at the target sequence enzyme cutting sites may be the cause. This could be due to issues with the setting of protective bases near the enzyme cutting sites when designing the PCR primers, leading to another ligation failure. As such, we plan to redesign the PCR primers and increase the number of protective bases on both sides of the enzyme cutting sites from one or none to three to improve the success rate of the ligation."}]},{"type":"element","tag":"h4","props":{"id":"circle-3-redesign-the-primers-increase-protective-bases-and-reconstruct-pet28-reca-hrp-egfp"},"children":[{"type":"text","value":"Circle 3: Redesign the primers, increase protective bases, and reconstruct pET28-recA-HRP-egfp"}]},{"type":"element","tag":"h5","props":{"id":"design-3"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Based on the experience learned from Circle 2, we have redesigned the PCR primers for the target fragment and added protective bases to them, and we are reconstructing the pET28-recA-HRP-egfp plasmid."}]},{"type":"element","tag":"h5","props":{"id":"build-3"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​After changing to new PCR primers and setting the protective bases on both sides of the restriction enzyme cutting sites to 3, the construction was carried out. Genetic circuits: recA-HRP-egfp were synthesized by Sangon Biotech according the BIOBRICK standard, the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir3","src":"https://static.igem.wiki/teams/5358/engineering-success/cir3.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 7. built result of circle 3"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nThe agarose gel electrophoresis results showed that after redesigning the PCR primers and increasing the number of protective bases on both sides of the restriction enzyme cutting sites from one or none to three, only one band was observed for the target fragment, indicating that the new PCR primers have strong specificity and the target fragment was successfully obtained.\n​\tHowever, due to time constraints, we did not proceed with the subsequent experiments."}]},{"type":"element","tag":"h5","props":{"id":"test-3"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Limited by time, we were unable to complete the reconstruction, and therefore have no test results."}]},{"type":"element","tag":"h5","props":{"id":"learn-3"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In this cycle, the new PCR primers and the addition of protective bases at both ends of the restriction enzyme sites are beneficial for improving the success rate of enzyme ligation, thereby providing an experimental basis for the successful construction of the gate circuit."}]},{"type":"element","tag":"h2","props":{"id":"hardware"},"children":[{"type":"text","value":"Hardware"}]},{"type":"element","tag":"h4","props":{"id":"objectives"},"children":[{"type":"text","value":"Objectives"}]},{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To provide a high-throughput portable reaction vessel."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To provide stable physical conditions for detection."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"High-sensitivity fluorescence detection."}]}]},{"type":"element","tag":"h4","props":{"id":"design-4"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Our entire device mainly consists of three major parts: the outer casing that encases the entire device, the reaction area located in the upper layer, and the detection area situated in the lower layer. In the initial design, we utilized an STM32 chip as the main controller to regulate temperature control, fluorescence detection, and Bluetooth communication functions. In the second iteration, we replaced the main controller with an ESP32 chip that integrates a wireless communication module, and we adopted integrated temperature control and detection modules, which further enhances the system's stability and accuracy. To meet the need for simultaneous detection of multiple samples, we also redesigned the high-throughput reaction chambers, achieving rapid successive detection through a linear slide rail system."}]},{"type":"element","tag":"table","props":{},"children":[{"type":"element","tag":"thead","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"th","props":{},"children":[]},{"type":"element","tag":"th","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Initial Scheme"}]}]}]},{"type":"element","tag":"th","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Final Scheme"}]}]}]},{"type":"element","tag":"th","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Advantages After Improvement"}]}]}]}]}]},{"type":"element","tag":"tbody","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"MCU"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"STM32F103C8T6"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Arduino Nano ESP32"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Integrate Bluetooth communication module, reduce the complexity of the design"}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Temperature Control"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"TB6612FNG"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"TCM1040"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"More precise control of temperature"}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Fluorescence Detection"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"AS7341"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"FM-4A"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Integrate excitation light and fluorescent sensor to make the detection result more stable."}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Reaction Platform"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Single Reaction Tube"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Slide Rail Platform"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Achieve high-throughput detection"}]}]}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["tab-title"]},"children":[{"type":"text","value":"Table 1. Scheme difference"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nIn terms of material selection, we have adopted a black silicone kit and an aluminum alloy temperature-controlled slot as the environment for the centrifuge tubes. This scheme can excellently meet the insulation requirements of the design."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Edition Scheme Process","src":"https://static.igem.wiki/teams/5358/engineering-success/edition-scheme-process.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 8. Edition Scheme Process"}]}]},{"type":"element","tag":"h4","props":{"id":"establishment"},"children":[{"type":"text","value":"Establishment"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\tThe system construction utilized common integrated modules, with the main controller being an ESP32 chip, and temperature control and detection were accomplished using the TCM temperature control module and LIDE Technology's FM series digital fluorescence detection module, respectively. We employed industrial processing techniques to fabricate the aluminum alloy temperature-controlled slots, while the casing and other components were created using 3D printing technology."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Schematic Diagram of Hardware Equipment","src":"https://static.igem.wiki/teams/5358/engineering-success/new.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 9. Schematic Diagram of Hardware Equipment"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Model Diagram of Hardware Equipment","src":"https://static.igem.wiki/teams/5358/engineering-success/model-diagram-of-hardware-equipment.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 10. Model Diagram of Hardware Equipment"}]}]},{"type":"element","tag":"h4","props":{"id":"test-4"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\tAfter finalizing the initial plan, we first carried out feasibility tests to verify the functionality of the fluorescence system and the temperature control system. After assembling the initial version of the model, numerous issues were identified through usage testing and preliminary risk analysis, such as the susceptibility of the fluorescence system to interference and the instability of the temperature control system. Through a cycle of testing, feedback, improvement, and retesting, the final version of the model demonstrated excellent performance across all aspects. Experiments showed that fluorescence identification was highly accurate, and the overall aesthetics were significantly enhanced."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Results of Temperature Control Experiment","src":"https://static.igem.wiki/teams/5358/engineering-success/results-of-temperature-control-experiment.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 11. Results of Temperature Control Experiment"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Results of Flurescence Verification Experiment","src":"https://static.igem.wiki/teams/5358/engineering-success/results-of-flurescence-verification-experiment.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 12. Results of Flurescence Verification Experiment"}]}]},{"type":"element","tag":"h4","props":{"id":"learn-4"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\tDuring the process of designing the device, we encountered various challenges, such as overly complex control systems, insufficient stability, inability to conduct multi-sample testing, and issues with the optical path design. Throughout the real-time testing and discussion phases, we continuously documented various issues, proposed improvement plans, and actively sought help from knowledgeable teachers and consultants. Eventually, we completed a hardware design that met the requirements. Through continuous learning, improvement, and questioning, our expertise in the relevant fields has been greatly boosted."}]},{"type":"element","tag":"h2","props":{"id":"software"},"children":[{"type":"text","value":"Software"}]},{"type":"element","tag":"h4","props":{"id":"objectives-1"},"children":[{"type":"text","value":"Objectives"}]},{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To implement human-machine interaction for information exchange with hardware."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To automatically process data sent by the hardware."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To visualize data."}]}]},{"type":"element","tag":"h4","props":{"id":"design-5"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​The main body is divided into two parts—the Bluetooth reception module and the plotting module."}]},{"type":"element","tag":"h4","props":{"id":"bluetooth-module-receives-data"},"children":[{"type":"text","value":"Bluetooth module receives data"}]},{"type":"element","tag":"pre","props":{"code":"CREATE discoveryAgent AS new BluetoothDeviceDiscoveryAgent\n​\nCONNECT discoveryAgent.deviceDiscovered TO this.deviceDiscovered\nCONNECT discoveryAgent.finished TO this.onDeviceConnected\n​\n// Start searching for Bluetooth devices\nCALL discoveryAgent.start()\n​\n// Initialize Bluetooth Socket\nCREATE socket AS new BluetoothSocket USING RfcommProtocol\n​\n// Connect button click signal to send STARTSTART command\nCONNECT ui.pushButton.clicked TO this.sendStartSignal\n"},"children":[{"type":"element","tag":"code","props":{"__ignoreMap":""},"children":[{"type":"text","value":"CREATE discoveryAgent AS new BluetoothDeviceDiscoveryAgent\n​\nCONNECT discoveryAgent.deviceDiscovered TO this.deviceDiscovered\nCONNECT discoveryAgent.finished TO this.onDeviceConnected\n​\n// Start searching for Bluetooth devices\nCALL discoveryAgent.start()\n​\n// Initialize Bluetooth Socket\nCREATE socket AS new BluetoothSocket USING RfcommProtocol\n​\n// Connect button click signal to send STARTSTART command\nCONNECT ui.pushButton.clicked TO this.sendStartSignal\n"}]}]},{"type":"element","tag":"h4","props":{"id":"plotting-module-performs-data-visualization"},"children":[{"type":"text","value":"Plotting module performs data visualization"}]},{"type":"element","tag":"pre","props":{"code":"FUNCTION updateGraphs()\n  // Create canvas\n  CREATE new scene\n  SET scene to graphicsView\n​\n  // Draw temperature graph\n  FOR i FROM 1 TO size of temperatureData - 1 DO\n    ADD line FROM (i-1, temperatureData[i-1]) TO (i, temperatureData[i]) WITH color red\n​\n  // Draw fluorescence intensity graph\n  FOR i FROM 1 TO size of fluorescenceData - 1 DO\n    ADD line FROM (i-1, fluorescenceData[i-1]) TO (i, fluorescenceData[i]) WITH color blue\n​\n  UPDATE graphicsView\nEND FUNCTION\n"},"children":[{"type":"element","tag":"code","props":{"__ignoreMap":""},"children":[{"type":"text","value":"FUNCTION updateGraphs()\n  // Create canvas\n  CREATE new scene\n  SET scene to graphicsView\n​\n  // Draw temperature graph\n  FOR i FROM 1 TO size of temperatureData - 1 DO\n    ADD line FROM (i-1, temperatureData[i-1]) TO (i, temperatureData[i]) WITH color red\n​\n  // Draw fluorescence intensity graph\n  FOR i FROM 1 TO size of fluorescenceData - 1 DO\n    ADD line FROM (i-1, fluorescenceData[i-1]) TO (i, fluorescenceData[i]) WITH color blue\n​\n  UPDATE graphicsView\nEND FUNCTION\n"}]}]},{"type":"element","tag":"h4","props":{"id":"data-segmentation-module"},"children":[{"type":"text","value":"Data segmentation module"}]},{"type":"element","tag":"pre","props":{"code":"FUNCTION splitAndFindMax(dataPoints, zeroThresholdCount)\n    INITIALIZE maxValues AS empty list\n    INITIALIZE currentSegment AS empty list\n    SET zeroCount TO 0\n​\n    FOR each point IN dataPoints DO\n        IF point.value == 0.0 THEN\n            INCREMENT zeroCount\n            IF zeroCount >= zeroThresholdCount THEN\n                IF currentSegment is not empty THEN\n                    SET maxVal TO maximum value in currentSegment\n                    ADD maxVal TO maxValues\n                    CLEAR currentSegment\n                    SET zeroCount TO 0  // Reset the counter​\n                CONTINUE  // Skip the current point, do not include in currentSegment​\n            END IF\n        ELSE\n            SET zeroCount TO 0  // Reset the counter because a non-zero value was encountered\n        END IF\n​\n        //Add the current point to the currentSegment\n        ADD point TO currentSegment\n    END FOR\n​\n    // Process the last segment\n    IF currentSegment is not empty THEN\n        SET maxVal TO maximum value in currentSegment\n        ADD maxVal TO maxValues\n    END IF\n    RETURN maxValues\nEND FUNCTION\n"},"children":[{"type":"element","tag":"code","props":{"__ignoreMap":""},"children":[{"type":"text","value":"FUNCTION splitAndFindMax(dataPoints, zeroThresholdCount)\n    INITIALIZE maxValues AS empty list\n    INITIALIZE currentSegment AS empty list\n    SET zeroCount TO 0\n​\n    FOR each point IN dataPoints DO\n        IF point.value == 0.0 THEN\n            INCREMENT zeroCount\n            IF zeroCount >= zeroThresholdCount THEN\n                IF currentSegment is not empty THEN\n                    SET maxVal TO maximum value in currentSegment\n                    ADD maxVal TO maxValues\n                    CLEAR currentSegment\n                    SET zeroCount TO 0  // Reset the counter​\n                CONTINUE  // Skip the current point, do not include in currentSegment​\n            END IF\n        ELSE\n            SET zeroCount TO 0  // Reset the counter because a non-zero value was encountered\n        END IF\n​\n        //Add the current point to the currentSegment\n        ADD point TO currentSegment\n    END FOR\n​\n    // Process the last segment\n    IF currentSegment is not empty THEN\n        SET maxVal TO maximum value in currentSegment\n        ADD maxVal TO maxValues\n    END IF\n    RETURN maxValues\nEND FUNCTION\n"}]}]},{"type":"element","tag":"h4","props":{"id":"testing"},"children":[{"type":"text","value":"Testing"}]}],"toc":{"title":"","searchDepth":1,"depth":3,"links":[{"id":"biosensor","depth":2,"text":"Biosensor","children":[{"id":"luminescence","depth":3,"text":"Luminescence","children":[{"id":"circle-design-reca-bsfbfp","depth":4,"text":"Circle: Design recA-BsFbFp"}]},{"id":"and-gate-design","depth":3,"text":"AND Gate Design","children":[{"id":"circle-1-design-pet28-reca-hrp-egfp","depth":4,"text":"Circle 1: Design pET28-recA-HRP-egfp"},{"id":"circle-2-adjust-the-ligation-system-and-reconstruct-the-plasmid-pet28-reca-hrp-egfp","depth":4,"text":"Circle 2: Adjust the ligation system and reconstruct the plasmid pET28-recA-HRP-egfp."},{"id":"circle-3-redesign-the-primers-increase-protective-bases-and-reconstruct-pet28-reca-hrp-egfp","depth":4,"text":"Circle 3: Redesign the primers, increase protective bases, and reconstruct pET28-recA-HRP-egfp"}]}]},{"id":"hardware","depth":2,"text":"Hardware","children":[{"id":"objectives","depth":4,"text":"Objectives"},{"id":"design-4","depth":4,"text":"Design"},{"id":"establishment","depth":4,"text":"Establishment"},{"id":"test-4","depth":4,"text":"Test"},{"id":"learn-4","depth":4,"text":"Learn"}]},{"id":"software","depth":2,"text":"Software","children":[{"id":"objectives-1","depth":4,"text":"Objectives"},{"id":"design-5","depth":4,"text":"Design"},{"id":"bluetooth-module-receives-data","depth":4,"text":"Bluetooth module receives data"},{"id":"plotting-module-performs-data-visualization","depth":4,"text":"Plotting module performs data visualization"},{"id":"data-segmentation-module","depth":4,"text":"Data segmentation module"},{"id":"testing","depth":4,"text":"Testing"}]}]}},"_type":"markdown","_id":"content:project-engineering.md","_source":"content","_file":"project-engineering.md","_stem":"project-engineering","_extension":"md"}
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+{"_path":"/project-engineering","_dir":"","_draft":false,"_partial":false,"_locale":"","title":"Project Engineering","description":"","body":{"type":"root","children":[{"type":"element","tag":"h2","props":{"id":"biosensor"},"children":[{"type":"text","value":"Biosensor"}]},{"type":"element","tag":"h3","props":{"id":"luminescence"},"children":[{"type":"text","value":"Luminescence"}]},{"type":"element","tag":"h4","props":{"id":"circle-design-reca-bsfbfp"},"children":[{"type":"text","value":"Circle: Design recA-BsFbFp"}]},{"type":"element","tag":"h5","props":{"id":"design"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In order to adapt to the low oxygen environment of the hardware design, we replaced the egfp gene, which strictly requires oxygen molecules as a cofactor for its luminescence, with the reporter gene BsFbFp. This gene can effectively express fluorescent signals in low or anaerobic environments."}]},{"type":"element","tag":"h5","props":{"id":"build"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"BsFbFp circuits","src":"https://static.igem.wiki/teams/5358/engineering-success/bsfbfp-circuits.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 1. BsFbFp circuits"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Genetic circuit: Gene recA and BsFbFp were all synthesized by Sangon Biotech according to BIOBRICK standard, and the gene circuit was obtained by PCR and verified by agarose gel electrophoresis."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"build results of BsFbFp ","src":"https://static.igem.wiki/teams/5358/engineering-success/build-results-of-bsfbfp.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 2. Build results of BsFbfP"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Construction of plasmid: The results of the agarose gel electrophoresis successfully showed bands for recA, the vector and BsFbFp, confirming that the gene circuit was successfully connected to the vector."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Ideal Strain: The successfully constructed plasmid was transformed into BL21 competent cells and cultured on LB solid medium with ampicillin."}]},{"type":"element","tag":"h5","props":{"id":"test"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Adding 600μL of bacterial solution and 30μL of ampicillin to 30mL of fresh culture medium, logarithmic growth was performed. Then, NA (Nalidixic Acid) was added in a gradient based on concentration to induce the bacteria. Subsequently, 250μL from each group was added to a anaerobic chamber and incubated on a shaking incubator at 37°C with a speed of 180 r/min. After 2 hours, the OD value and fluorescence intensity were measured."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"RFU-BsFbFp in 10lianpaichip(anaerobic chamber)","src":"https://static.igem.wiki/teams/5358/engineering-success/rfu-bsfbfp-in-10lianpaichip-anaerobic-chamber.jpg"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 3. RFU-BsFbFp in 10lianpaichip(anaerobic chamber)"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​The RFU (Relative Fluorescence Units) of the three groups of engineered bacteria increased linearly with the amount of NA (Nalidixic Acid) added. The results indicate that the fluorescent expression circuit of BsFbFp has been successfully constructed, and the engineered bacteria can effectively express fluorescence in an anaerobic environment."}]},{"type":"element","tag":"h5","props":{"id":"learn"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In conclusion，the plasmid constructed using BsFbFp as a reporter gene can effectively express fluorescence in low-oxygen or anaerobic environments, which is beneficial for the design of the hardware components."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​So we believed that the work in this cycle has been successfully accomplished., and we obtained the engineered bacteria needed for our goals."}]},{"type":"element","tag":"h3","props":{"id":"and-gate-design"},"children":[{"type":"text","value":"AND Gate Design"}]},{"type":"element","tag":"h4","props":{"id":"circle-1-design-pet28-reca-hrp-egfp"},"children":[{"type":"text","value":"Circle 1: Design pET28-recA-HRP-egfp"}]},{"type":"element","tag":"h5","props":{"id":"design-1"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​To construct the and gate, we introduced the hrp system, composed of the hrpR gene and hrpS gene, as well as the HrpL promoter regulated by the sigma54 factor. The hrpL promoter will only be activated when both the hrpR gene and the hrpS gene are expressed simultaneously."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In 2023, the BIT team successfully constructed P recA, the Hrp system, and egfp on the pUC19 plasmid. However, tests showed that the expression level of the circuit constructed using the pUC19 plasmid as a vector was relatively low, significantly deviating from the expected results. Therefore, we plan to switch to the pET28 plasmid, which has a more prominent expression level, to facilitate the synergistic assessment of endocrine disruption effects and oxidative damage effects."}]},{"type":"element","tag":"h5","props":{"id":"build-1"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Genetic circuit: recA-HRP-egfp were synthesized by Sangon Biotech according to BIOBRICK standard， the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir1-1","src":"https://static.igem.wiki/teams/5358/engineering-success/cir1-1.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 4. enzyme digestion result of circle 1"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nThe results of the agarose gel electrophoresis indicated that the vector was successfully digested and the target fragment was successfully amplified by PCR. The ligation was carried out at a 1:4 ratio of vector to target fragment, followed by transformation into DH5α competent cells and verification through agarose gel electrophoresis."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir1-2","src":"https://static.igem.wiki/teams/5358/engineering-success/cir1-2.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 5. build result of circle 1"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nVerification results: In multiple rounds of construction validation experiments, the target fragment showed no band in the agarose gel electrophoresis results, and the restriction enzyme digestion verification indicated self-ligation of the vector, proving that the construction in this cycle has failed."}]},{"type":"element","tag":"h5","props":{"id":"test-1"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Due to the failure of the target plasmid construction in this cycle, it is necessary to analyze the issues present in the plasmid design and construction process and make corrections."}]},{"type":"element","tag":"h5","props":{"id":"learn-1"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​During this construction process, we overlooked the reagent concentration ratio of the vector to the target fragment in our ligation system, which may be a major factor contributing to the failure of ligation. We plan to introduce the concentration factor of the ligation reagents into the ratio to improve the efficiency of ligation."}]},{"type":"element","tag":"h4","props":{"id":"circle-2-adjust-the-ligation-system-and-reconstruct-the-plasmid-pet28-reca-hrp-egfp"},"children":[{"type":"text","value":"Circle 2: Adjust the ligation system and reconstruct the plasmid pET28-recA-HRP-egfp."}]},{"type":"element","tag":"h5","props":{"id":"design-2"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Based on the experience learned from Circle 1, we plan to use a new ligation system, increase the dosage of the target fragment, and improve the ligation efficiency."}]},{"type":"element","tag":"h5","props":{"id":"build-2"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​The construction process used three different ligation systems, with the ratios of vector to target fragment being 1:4, 1:5, and 1:6, respectively. The constructed plasmids were extracted and verified by PCR followed by agarose gel electrophoresis."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir2","src":"https://static.igem.wiki/teams/5358/engineering-success/cir2.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 6. built result of circle 2"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nConstruction of plasmid: In the multi-round adjustment experiments of the ligation system construction, the agarose gel electrophoresis results still did not show a band for the target fragment, indicating that the construction in this cycle was unsuccessful."}]},{"type":"element","tag":"h5","props":{"id":"test-2"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Due to the failure of target plasmid construction in this cycle, testing cannot be carried out."}]},{"type":"element","tag":"h5","props":{"id":"learn-2"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In this cycle, we have corrected the ligation system, removing this influencing factor. Therefore, we suspect that the low partial digestion efficiency at the target sequence enzyme cutting sites may be the cause. This could be due to issues with the setting of protective bases near the enzyme cutting sites when designing the PCR primers, leading to another ligation failure. As such, we plan to redesign the PCR primers and increase the number of protective bases on both sides of the enzyme cutting sites from one or none to three to improve the success rate of the ligation."}]},{"type":"element","tag":"h4","props":{"id":"circle-3-redesign-the-primers-increase-protective-bases-and-reconstruct-pet28-reca-hrp-egfp"},"children":[{"type":"text","value":"Circle 3: Redesign the primers, increase protective bases, and reconstruct pET28-recA-HRP-egfp"}]},{"type":"element","tag":"h5","props":{"id":"design-3"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Based on the experience learned from Circle 2, we have redesigned the PCR primers for the target fragment and added protective bases to them, and we are reconstructing the pET28-recA-HRP-egfp plasmid."}]},{"type":"element","tag":"h5","props":{"id":"build-3"},"children":[{"type":"text","value":"Build"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​After changing to new PCR primers and setting the protective bases on both sides of the restriction enzyme cutting sites to 3, the construction was carried out. Genetic circuits: recA-HRP-egfp were synthesized by Sangon Biotech according the BIOBRICK standard, the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Cir3","src":"https://static.igem.wiki/teams/5358/engineering-success/cir3.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 7. built result of circle 3"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nThe agarose gel electrophoresis results showed that after redesigning the PCR primers and increasing the number of protective bases on both sides of the restriction enzyme cutting sites from one or none to three, only one band was observed for the target fragment, indicating that the new PCR primers have strong specificity and the target fragment was successfully obtained.\n​\nHowever, due to time constraints, we did not proceed with the subsequent experiments."}]},{"type":"element","tag":"h5","props":{"id":"test-3"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Limited by time, we were unable to complete the reconstruction, and therefore have no test results."}]},{"type":"element","tag":"h5","props":{"id":"learn-3"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​In this cycle, the new PCR primers and the addition of protective bases at both ends of the restriction enzyme sites are beneficial for improving the success rate of enzyme ligation, thereby providing an experimental basis for the successful construction of the gate circuit."}]},{"type":"element","tag":"h2","props":{"id":"hardware"},"children":[{"type":"text","value":"Hardware"}]},{"type":"element","tag":"h4","props":{"id":"objectives"},"children":[{"type":"text","value":"Objectives"}]},{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To provide a high-throughput portable reaction vessel."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To provide stable physical conditions for detection."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"High-sensitivity fluorescence detection."}]}]},{"type":"element","tag":"h4","props":{"id":"design-4"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​Our entire device mainly consists of three major parts: the outer casing that encases the entire device, the reaction area located in the upper layer, and the detection area situated in the lower layer. In the initial design, we utilized an STM32 chip as the main controller to regulate temperature control, fluorescence detection, and Bluetooth communication functions. In the second iteration, we replaced the main controller with an ESP32 chip that integrates a wireless communication module, and we adopted integrated temperature control and detection modules, which further enhances the system's stability and accuracy. To meet the need for simultaneous detection of multiple samples, we also redesigned the high-throughput reaction chambers, achieving rapid successive detection through a linear slide rail system."}]},{"type":"element","tag":"table","props":{},"children":[{"type":"element","tag":"thead","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"th","props":{},"children":[]},{"type":"element","tag":"th","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Initial Scheme"}]}]}]},{"type":"element","tag":"th","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Final Scheme"}]}]}]},{"type":"element","tag":"th","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Advantages After Improvement"}]}]}]}]}]},{"type":"element","tag":"tbody","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"MCU"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"STM32F103C8T6"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Arduino Nano ESP32"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Integrate Bluetooth communication module, reduce the complexity of the design"}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Temperature Control"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"TB6612FNG"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"TCM1040"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"More precise control of temperature"}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Fluorescence Detection"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"AS7341"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"FM-4A"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Integrate excitation light and fluorescent sensor to make the detection result more stable."}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"element","tag":"em","props":{},"children":[{"type":"element","tag":"strong","props":{},"children":[{"type":"text","value":"Reaction Platform"}]}]}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Single Reaction Tube"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Slide Rail Platform"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Achieve high-throughput detection"}]}]}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["tab-title"]},"children":[{"type":"text","value":"Table 1. Scheme difference"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​\nIn terms of material selection, we have adopted a black silicone kit and an aluminum alloy temperature-controlled slot as the environment for the centrifuge tubes. This scheme can excellently meet the insulation requirements of the design."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Edition Scheme Process","src":"https://static.igem.wiki/teams/5358/engineering-success/edition-scheme-process.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 8. Edition Scheme Process"}]}]},{"type":"element","tag":"h4","props":{"id":"establishment"},"children":[{"type":"text","value":"Establishment"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​The system construction utilized common integrated modules, with the main controller being an ESP32 chip, and temperature control and detection were accomplished using the TCM temperature control module and LIDE Technology's FM series digital fluorescence detection module, respectively. We employed industrial processing techniques to fabricate the aluminum alloy temperature-controlled slots, while the casing and other components were created using 3D printing technology."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Schematic Diagram of Hardware Equipment","src":"https://static.igem.wiki/teams/5358/engineering-success/new.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 9. Schematic Diagram of Hardware Equipment"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Model Diagram of Hardware Equipment","src":"https://static.igem.wiki/teams/5358/engineering-success/model-diagram-of-hardware-equipment.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 10. Model Diagram of Hardware Equipment"}]}]},{"type":"element","tag":"h4","props":{"id":"test-4"},"children":[{"type":"text","value":"Test"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​After finalizing the initial plan, we first carried out feasibility tests to verify the functionality of the fluorescence system and the temperature control system. After assembling the initial version of the model, numerous issues were identified through usage testing and preliminary risk analysis, such as the susceptibility of the fluorescence system to interference and the instability of the temperature control system. Through a cycle of testing, feedback, improvement, and retesting, the final version of the model demonstrated excellent performance across all aspects. Experiments showed that fluorescence identification was highly accurate, and the overall aesthetics were significantly enhanced."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Results of Temperature Control Experiment","src":"https://static.igem.wiki/teams/5358/engineering-success/results-of-temperature-control-experiment.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 11. Results of Temperature Control Experiment"}]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"img","props":{"alt":"Results of Flurescence Verification Experiment","src":"https://static.igem.wiki/teams/5358/engineering-success/results-of-flurescence-verification-experiment.png"},"children":[]}]},{"type":"element","tag":"p","props":{},"children":[{"type":"element","tag":"span","props":{"className":["fig-title"]},"children":[{"type":"text","value":"Figure 12. Results of Flurescence Verification Experiment"}]}]},{"type":"element","tag":"h4","props":{"id":"learn-4"},"children":[{"type":"text","value":"Learn"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​During the process of designing the device, we encountered various challenges, such as overly complex control systems, insufficient stability, inability to conduct multi-sample testing, and issues with the optical path design. Throughout the real-time testing and discussion phases, we continuously documented various issues, proposed improvement plans, and actively sought help from knowledgeable teachers and consultants. Eventually, we completed a hardware design that met the requirements. Through continuous learning, improvement, and questioning, our expertise in the relevant fields has been greatly boosted."}]},{"type":"element","tag":"h2","props":{"id":"software"},"children":[{"type":"text","value":"Software"}]},{"type":"element","tag":"h4","props":{"id":"objectives-1"},"children":[{"type":"text","value":"Objectives"}]},{"type":"element","tag":"ol","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To implement human-machine interaction for information exchange with hardware."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To automatically process data sent by the hardware."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"To visualize data."}]}]},{"type":"element","tag":"h4","props":{"id":"design-5"},"children":[{"type":"text","value":"Design"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"​The main body is divided into two 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discoveryAgent.finished TO this.onDeviceConnected\n​\n// Start searching for Bluetooth devices\nCALL discoveryAgent.start()\n​\n// Initialize Bluetooth Socket\nCREATE socket AS new BluetoothSocket USING RfcommProtocol\n​\n// Connect button click signal to send STARTSTART command\nCONNECT ui.pushButton.clicked TO this.sendStartSignal\n"}]}]},{"type":"element","tag":"h4","props":{"id":"plotting-module-performs-data-visualization"},"children":[{"type":"text","value":"Plotting module performs data visualization"}]},{"type":"element","tag":"pre","props":{"code":"FUNCTION updateGraphs()\n  // Create canvas\n  CREATE new scene\n  SET scene to graphicsView\n​\n  // Draw temperature graph\n  FOR i FROM 1 TO size of temperatureData - 1 DO\n    ADD line FROM (i-1, temperatureData[i-1]) TO (i, temperatureData[i]) WITH color red\n​\n  // Draw fluorescence intensity graph\n  FOR i FROM 1 TO size of fluorescenceData - 1 DO\n    ADD line FROM (i-1, fluorescenceData[i-1]) TO (i, fluorescenceData[i]) WITH color blue\n​\n  UPDATE graphicsView\nEND FUNCTION\n"},"children":[{"type":"element","tag":"code","props":{"__ignoreMap":""},"children":[{"type":"text","value":"FUNCTION updateGraphs()\n  // Create canvas\n  CREATE new scene\n  SET scene to graphicsView\n​\n  // Draw temperature graph\n  FOR i FROM 1 TO size of temperatureData - 1 DO\n    ADD line FROM (i-1, temperatureData[i-1]) TO (i, temperatureData[i]) WITH color red\n​\n  // Draw fluorescence intensity graph\n  FOR i FROM 1 TO size of fluorescenceData - 1 DO\n    ADD line FROM (i-1, fluorescenceData[i-1]) TO (i, fluorescenceData[i]) WITH color blue\n​\n  UPDATE graphicsView\nEND FUNCTION\n"}]}]},{"type":"element","tag":"h4","props":{"id":"data-segmentation-module"},"children":[{"type":"text","value":"Data segmentation module"}]},{"type":"element","tag":"pre","props":{"code":"FUNCTION splitAndFindMax(dataPoints, zeroThresholdCount)\n    INITIALIZE maxValues AS empty list\n    INITIALIZE currentSegment AS empty list\n    SET 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The operation steps are as follows :"}]},{"type":"element","tag":"ul","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Open the water bath in advance and set the temperature to 75°C(167°F);"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Use Agarose gel electrophoresis to verify the DNA fragment and observe in the gel imaging system. Cut the agarose gel containing the target band with a knife under the ultraviolet lamp, and record the weight of the 1.5 mL centrifuge tube in advance. After the centrifuge tube is loaded, weigh it again and calculate the gel weight. 100 mg is a gel volume equivalent to 100 μL;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Add 3 gel volume of gel buffer DE-A, mix well and put into 75°C water bath heating 6-8 min, mix it up and down every 2 min during water bath;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Add 0.5 DE-A volume of gel buffer DE-B, uniform mix it, and add 1 gel volume of isopropanol;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Purify the resulting products by centrifugation. Transfer the above solution to an adsorption column and place it in a 2mL centrifuge tube. Centrifuge at 12,000 rpm for 1 min, discard the waste liquid, and place the adsorption column back into the collection tube;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Add 500 μL buffer W1 to the adsorption column, centrifuge at 12,000 rpm for 30s, pour the waste liquid, and place the adsorption column back into the collection tube;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Add 700 μL buffer W2 to the adsorption column, centrifuge at 12,000 rpm for 30s, pour the waste liquid and place the adsorption column back into the collection tube, and repeat the operation once again;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Put the adsorption column in a new 1.5ml centrifuge tube, add 30μl Eluent in the center of the adsorption column membrane, and then centrifuge at the 12,000rpm for 1min after standing at room temperature, from which electrophoretic products were obtained;"}]}]},{"type":"element","tag":"h4","props":{"id":"plasmid-construction"},"children":[{"type":"text","value":"Plasmid Construction"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"The obtained gene line fragment and pUC19 empty vector are digested and ligated by enzyme ligation."}]},{"type":"element","tag":"h5","props":{"id":"enzyme-digestion"},"children":[{"type":"text","value":"Enzyme digestion"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Material"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Incubator, (0.2ml)PCR tube, pipetting gun, pipette tip, restriction enzyme, NEB Buffer 3.1, target gene"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Protocol"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"After adding the following enzyme digestion system to a 0.2 mL centrifuge tube, incubate it in an incubator at 37°C for 2 h."}]},{"type":"element","tag":"table","props":{},"children":[{"type":"element","tag":"thead","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"th","props":{},"children":[{"type":"text","value":"Enzyme digestion system"}]},{"type":"element","tag":"th","props":{},"children":[{"type":"text","value":"Volume"}]}]}]},{"type":"element","tag":"tbody","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Plasmid"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"17μL"}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"NEB 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Ligation"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Material"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Incubator, (0.2ml) PCR tubes, pipetting gun, pipette tip, T4 DNA ligase Buffer 10x, Nuclease-Free Water, T4 DNA ligase, vector fragment, target fragment;"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Protocol"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"After adding the following ligation system to a 0.2mL centrifuge tube, incubate it in an incubator at 37°C for 24 h."}]},{"type":"element","tag":"table","props":{},"children":[{"type":"element","tag":"thead","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"th","props":{},"children":[{"type":"text","value":"Connection system"}]},{"type":"element","tag":"th","props":{},"children":[{"type":"text","value":"Volume"}]}]}]},{"type":"element","tag":"tbody","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Vector fragment"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"3μL"}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Target fragment"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"12μL"}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"T4 DNA ligase Buffer"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"2.5μL"}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Nuclease-Free water"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"6.5μL"}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"T4 DNA ligase"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"1μL"}]}]},{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"Total"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"25μL"}]}]}]}]},{"type":"element","tag":"h5","props":{"id":"transformation-and-selection"},"children":[{"type":"text","value":"Transformation and Selection"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Transfer 5μL of the ligation product into 50μL of BL21 competent cells. After incubation, plate 100μL of the transformed cells onto LB agar plates containing 100μg/mL ampicillin resistance. Invert the plates and incubate overnight at 37°C. Pick single colonies and inoculate them into 30mL of antibiotic-free LB broth. Add 30μL of ampicillin and incubate at 37°C with shaking at 180r/min for 12 h. Preserve the cultures in glycerol at -10°C;"}]},{"type":"element","tag":"h5","props":{"id":"plasmid-transformation"},"children":[{"type":"text","value":"Plasmid transformation"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Material"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Vertical clean bench, shaker, water bath, centrifuge, pipetting gun, pipette tip, solid culture dish, alcohol lamp, applicator, ampicillin, LB-free liquid medium, competent cells;"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Protocol"}]},{"type":"element","tag":"ul","props":{},"children":[{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Fill the foam box with about 2/3 of the volume of ice, and take100μL of prepared competent cells. turn on the ultra-clean table UV lamp in advance for 15min for sterilization. After sterilization, light up the alcohol lamp in the ultra-clean table, and transfer 50μL competent cells into a new aseptic centrifuge tube with a pipette beside the alcohol lamp, and add 5μL plasmid solution into it."}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"place the centrifuge tube containing the plasmid solution in the ice box for 30min, turn on the water bath in advance and set the temperature at 42°C, heat the centrifuge tube after the ice bath in the water bath at 42°C for 45s, and immediately remove the centrifuge tube and place it in the ice box for 2min to complete the transformation process;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Take out the packaged liquid medium without anti-LB, and add 500μL liquid medium into the centrifuge tube after heating. Incubate the centrifuge tube in a shaking table at 37°C 180r/min for 1h;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"After removing the centrifuge tube from the shaker, centrifuge it at 7000rpm for 30 s. Carefully aspirate and discard the supernatant (400μL) next to an alcohol lamp. Resuspend the remaining bacterial pellet using a pipetting gun, and based on plasmid resistance, select solid medium containing ampicillin resistance. Using a pipetting gun next to an alcohol lamp, add 100μL of the resuspended bacterial culture onto the surface of the solid medium. Spread evenly using a spreader. Cover the petri dish and invert it, then place it in a 37°C incubator for 12 h;"}]},{"type":"element","tag":"li","props":{},"children":[{"type":"text","value":"Add 30μL of ampicillin to 30mL of antibiotic-free LB liquid medium. Using a pipette tip, pick a single colony and inoculate it into the aforementioned liquid medium. Incubate at 37°C with shaking at 180r/min for 12 h;"}]}]},{"type":"element","tag":"h3","props":{"id":"characterization-experiment"},"children":[{"type":"text","value":"Characterization experiment"}]},{"type":"element","tag":"h4","props":{"id":"preparation"},"children":[{"type":"text","value":"Preparation"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Transfer The bacteria solution cultured overnight to 30mL fresh non-resistant LB medium, with adding 30μL ampicillin, and culture in the shaking table at 37°C and 180r/min until the OD600 value of the bacteria solution is between 0.3 and 0.4;"}]},{"type":"element","tag":"h4","props":{"id":"experimental-characterization-of-the-anaerobic-environment"},"children":[{"type":"text","value":"Experimental characterization of the anaerobic environment"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Method 1：The bacterial solution was added to DNA damaging agent, dropped into anaerobic row and incubated for 2h in a 37 C incubator. Measure OD600 and fluorescence intensity of bacterial solution by microplate reader;"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Method 2：The bacterial solution was sealed using paraffin oil and DNA damaging agents were added under the paraffin oil page and incubated for 2h in a 37 C incubator. Measure OD600 and fluorescence intensity of bacterial solution by microplate reader;"}]},{"type":"element","tag":"table","props":{},"children":[{"type":"element","tag":"thead","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"th","props":{},"children":[{"type":"text","value":"DNA damaging reagent"}]},{"type":"element","tag":"th","props":{},"children":[{"type":"text","value":"Concentration gradient"}]},{"type":"element","tag":"th","props":{},"children":[]}]}]},{"type":"element","tag":"tbody","props":{},"children":[{"type":"element","tag":"tr","props":{},"children":[{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"NA"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"0μL"}]},{"type":"element","tag":"td","props":{},"children":[{"type":"text","value":"1μL 5μL 10μL 15μL 20μL 25μL"}]}]}]}]},{"type":"element","tag":"h4","props":{"id":"data-measurement"},"children":[{"type":"text","value":"Data measurement"}]},{"type":"element","tag":"h5","props":{"id":"measure-the-od600"},"children":[{"type":"text","value":"Measure the OD600"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Transfer 200μL of the bacteria solution to a 96-hole black microplate and place it in a microplate reader. Set the wavelength to 600 and measure the absorbance reading."}]},{"type":"element","tag":"h5","props":{"id":"measure-the-fluorescence-intensity"},"children":[{"type":"text","value":"Measure the Fluorescence Intensity"}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Transfer 200μL of the bacteria solution to a 96-hole black microplate and place it in a microplate reader. Set the excitation wavelength to 488 nm, emission wavelength to 520 nm, and gain to 75. Measure the fluorescence intensity."}]},{"type":"element","tag":"p","props":{},"children":[{"type":"text","value":"Each group of data was measured three times and averaged."}]}],"toc":{"title":"","searchDepth":1,"depth":3,"links":[{"id":"what-is-endocrine-pollutant","depth":2,"text":"What IS Endocrine Pollutant？"},{"id":"what-is-dna-damage","depth":2,"text":"What IS DNA Damage？"},{"id":"experiment","depth":2,"text":"Experiment","children":[{"id":"strain-obtain","depth":3,"text":"Strain Obtain","children":[{"id":"gene-acquisition","depth":4,"text":"gene acquisition"},{"id":"plasmid-construction","depth":4,"text":"Plasmid Construction"}]},{"id":"characterization-experiment","depth":3,"text":"Characterization experiment","children":[{"id":"preparation","depth":4,"text":"Preparation"},{"id":"experimental-characterization-of-the-anaerobic-environment","depth":4,"text":"Experimental characterization of the anaerobic environment"},{"id":"data-measurement","depth":4,"text":"Data measurement"}]}]}]}},"_type":"markdown","_id":"content:wetlab-experiment.md","_source":"content","_file":"wetlab-experiment.md","_stem":"wetlab-experiment","_extension":"md"}
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diff --git a/.output/public/api/_content/query/jxDrjMjVNP.1727868965354.json b/.output/public/api/_content/query/jxDrjMjVNP.1727871061049.json
similarity index 100%
rename from .output/public/api/_content/query/jxDrjMjVNP.1727868965354.json
rename to .output/public/api/_content/query/jxDrjMjVNP.1727871061049.json
diff --git a/.output/public/attributions/_payload.json b/.output/public/attributions/_payload.json
index eae442b8589a746d4256c78bcb8cd9ca782889c7..9a01cccae9610f007b3fc68b99a433f5800e0c88 100644
--- a/.output/public/attributions/_payload.json
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diff --git a/.output/public/attributions/index.html b/.output/public/attributions/index.html
index c349589f9ade90c20c465172e0f06f0f2175acdc..a7ce8f7dc469d616bba7fb9bd64e054aa49eb9ac 100644
--- a/.output/public/attributions/index.html
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.el-alert__description{color:var(--el-color-info)}.el-alert--info.is-dark{background-color:var(--el-color-info);color:var(--el-color-white)}.el-alert--warning{--el-alert-bg-color:var(--el-color-warning-light-9)}.el-alert--warning.is-light{background-color:var(--el-alert-bg-color)}.el-alert--warning.is-light,.el-alert--warning.is-light .el-alert__description{color:var(--el-color-warning)}.el-alert--warning.is-dark{background-color:var(--el-color-warning);color:var(--el-color-white)}.el-alert--error{--el-alert-bg-color:var(--el-color-error-light-9)}.el-alert--error.is-light{background-color:var(--el-alert-bg-color)}.el-alert--error.is-light,.el-alert--error.is-light .el-alert__description{color:var(--el-color-error)}.el-alert--error.is-dark{background-color:var(--el-color-error);color:var(--el-color-white)}.el-alert__content{display:flex;flex-direction:column;gap:4px}.el-alert .el-alert__icon{font-size:var(--el-alert-icon-size);margin-right:8px;width:var(--el-alert-icon-size)}.el-alert 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inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;display:inline-block;position:relative;width:var(--el-input-width)}.el-autocomplete__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-autocomplete__popper.el-popper,.el-autocomplete__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-border-color-light)}.el-autocomplete__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-autocomplete__popper.el-popper[data-popper-placement^=bottom] 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translateX(100%);z-index:var(--el-index-normal)}.el-badge__content.is-fixed.is-dot{right:5px}.el-badge__content.is-dot{border-radius:50%;height:8px;padding:0;right:0;width:8px}.el-badge__content--primary{background-color:var(--el-color-primary)}.el-badge__content--success{background-color:var(--el-color-success)}.el-badge__content--warning{background-color:var(--el-color-warning)}.el-badge__content--info{background-color:var(--el-color-info)}.el-badge__content--danger{background-color:var(--el-color-danger)}.el-breadcrumb{font-size:14px;line-height:1}.el-breadcrumb:after,.el-breadcrumb:before{content:"";display:table}.el-breadcrumb:after{clear:both}.el-breadcrumb__separator{color:var(--el-text-color-placeholder);font-weight:700;margin:0 9px}.el-breadcrumb__separator.el-icon{font-weight:400;margin:0 6px}.el-breadcrumb__separator.el-icon 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.el-button--info:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button{--el-button-font-weight:var(--el-font-weight-primary);--el-button-border-color:var(--el-border-color);--el-button-bg-color:var(--el-fill-color-blank);--el-button-text-color:var(--el-text-color-regular);--el-button-disabled-text-color:var(--el-disabled-text-color);--el-button-disabled-bg-color:var(--el-fill-color-blank);--el-button-disabled-border-color:var(--el-border-color-light);--el-button-divide-border-color:hsla(0,0%,100%,.5);--el-button-hover-text-color:var(--el-color-primary);--el-button-hover-bg-color:var(--el-color-primary-light-9);--el-button-hover-border-color:var(--el-color-primary-light-7);--el-button-active-text-color:var(--el-button-hover-text-color);--el-button-active-border-color:var(--el-color-primary);--el-button-active-bg-color:var(--el-button-hover-bg-color);--el-button-outline-color:var(--el-color-primary-light-5);--el-button-hover-link-text-color:var(--el-color-info);--el-button-active-color:var(--el-text-color-primary);align-items:center;-webkit-appearance:none;background-color:var(--el-button-bg-color);border:var(--el-border);border-color:var(--el-button-border-color);box-sizing:border-box;color:var(--el-button-text-color);cursor:pointer;display:inline-flex;font-weight:var(--el-button-font-weight);height:32px;justify-content:center;line-height:1;outline:none;text-align:center;transition:.1s;-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-button:hover{background-color:var(--el-button-hover-bg-color);border-color:var(--el-button-hover-border-color);color:var(--el-button-hover-text-color);outline:none}.el-button:active{background-color:var(--el-button-active-bg-color);border-color:var(--el-button-active-border-color);color:var(--el-button-active-text-color);outline:none}.el-button:focus-visible{outline:2px 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20px;position:relative}.el-cascader-node.is-selectable.in-active-path{color:var(--el-cascader-menu-text-color)}.el-cascader-node.in-active-path,.el-cascader-node.is-active,.el-cascader-node.is-selectable.in-checked-path{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader-node:not(.is-disabled){cursor:pointer}.el-cascader-node:not(.is-disabled):focus,.el-cascader-node:not(.is-disabled):hover{background:var(--el-cascader-node-background-hover)}.el-cascader-node.is-disabled{color:var(--el-cascader-node-color-disabled);cursor:not-allowed}.el-cascader-node__prefix{left:10px;position:absolute}.el-cascader-node__postfix{position:absolute;right:10px}.el-cascader-node__label{flex:1;overflow:hidden;padding:0 8px;text-align:left;text-overflow:ellipsis;white-space:nowrap}.el-cascader-node>.el-checkbox,.el-cascader-node>.el-radio{margin-right:0}.el-cascader-node>.el-radio .el-radio__label{padding-left:0}.el-cascader{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);display:inline-block;font-size:var(--el-font-size-base);line-height:32px;outline:none;position:relative;vertical-align:middle}.el-cascader:not(.is-disabled):hover .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset;cursor:pointer}.el-cascader .el-input{cursor:pointer;display:flex}.el-cascader .el-input .el-input__inner{cursor:pointer;text-overflow:ellipsis}.el-cascader .el-input .el-input__suffix-inner .el-icon{height:calc(100% - 2px)}.el-cascader .el-input .el-input__suffix-inner .el-icon svg{vertical-align:middle}.el-cascader .el-input .icon-arrow-down{font-size:14px;transition:transform var(--el-transition-duration)}.el-cascader .el-input .icon-arrow-down.is-reverse{transform:rotate(180deg)}.el-cascader .el-input .icon-circle-close:hover{color:var(--el-input-clear-hover-color,var(--el-text-color-secondary))}.el-cascader .el-input.is-focus .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-focus-border-color,var(--el-color-primary)) inset}.el-cascader--large{font-size:14px;line-height:40px}.el-cascader--small{font-size:12px;line-height:24px}.el-cascader.is-disabled .el-cascader__label{color:var(--el-disabled-text-color);z-index:calc(var(--el-index-normal) + 1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper .el-popper__arrow:before{border:var(--el-cascader-menu-border)}.el-cascader__dropdown.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper{box-shadow:var(--el-cascader-menu-shadow)}.el-cascader__tags{box-sizing:border-box;display:flex;flex-wrap:wrap;left:0;line-height:normal;position:absolute;right:30px;text-align:left;top:50%;transform:translateY(-50%)}.el-cascader__tags .el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags .el-tag{align-items:center;background:var(--el-fill-color);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag.el-tag--dark,.el-cascader__collapse-tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__collapse-tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__collapse-tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__collapse-tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__suggestion-panel{border-radius:var(--el-cascader-menu-radius)}.el-cascader__suggestion-list{color:var(--el-cascader-menu-text-color);font-size:var(--el-font-size-base);margin:0;max-height:204px;padding:6px 0;text-align:center}.el-cascader__suggestion-item{align-items:center;cursor:pointer;display:flex;height:34px;justify-content:space-between;outline:none;padding:0 15px;text-align:left}.el-cascader__suggestion-item:focus,.el-cascader__suggestion-item:hover{background:var(--el-cascader-node-background-hover)}.el-cascader__suggestion-item.is-checked{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader__suggestion-item>span{margin-right:10px}.el-cascader__empty-text{color:var(--el-cascader-color-empty);margin:10px 0}.el-cascader__search-input{background:transparent;border:none;box-sizing:border-box;color:var(--el-cascader-menu-text-color);flex:1;height:24px;margin:2px 0 2px 11px;min-width:60px;outline:none;padding:0}.el-cascader__search-input::-moz-placeholder{color:transparent}.el-cascader__search-input::placeholder{color:transparent}.el-check-tag{background-color:var(--el-color-info-light-9);border-radius:var(--el-border-radius-base);color:var(--el-color-info);cursor:pointer;display:inline-block;font-size:var(--el-font-size-base);font-weight:700;line-height:var(--el-font-size-base);padding:7px 15px;transition:var(--el-transition-all)}.el-check-tag:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--primary.is-checked{background-color:var(--el-color-primary-light-8);color:var(--el-color-primary)}.el-check-tag.el-check-tag--primary.is-checked:hover{background-color:var(--el-color-primary-light-7)}.el-check-tag.el-check-tag--primary.is-checked.is-disabled{background-color:var(--el-color-primary-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-checked.is-disabled:hover{background-color:var(--el-color-primary-light-8)}.el-check-tag.el-check-tag--primary.is-disabled{background-color:var(--el-color-info-light-9);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-check-tag.el-check-tag--success.is-checked{background-color:var(--el-color-success-light-8);color:var(--el-color-success)}.el-check-tag.el-check-tag--success.is-checked:hover{background-color:var(--el-color-success-light-7)}.el-check-tag.el-check-tag--success.is-checked.is-disabled{background-color:var(--el-color-success-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-checked.is-disabled:hover{background-color:var(--el-color-success-light-8)}.el-check-tag.el-check-tag--success.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-disabled,.el-check-tag.el-check-tag--success.is-disabled:hover{background-color:var(--el-color-success-light-9)}.el-check-tag.el-check-tag--warning.is-checked{background-color:var(--el-color-warning-light-8);color:var(--el-color-warning)}.el-check-tag.el-check-tag--warning.is-checked:hover{background-color:var(--el-color-warning-light-7)}.el-check-tag.el-check-tag--warning.is-checked.is-disabled{background-color:var(--el-color-warning-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-checked.is-disabled:hover{background-color:var(--el-color-warning-light-8)}.el-check-tag.el-check-tag--warning.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-disabled,.el-check-tag.el-check-tag--warning.is-disabled:hover{background-color:var(--el-color-warning-light-9)}.el-check-tag.el-check-tag--danger.is-checked{background-color:var(--el-color-danger-light-8);color:var(--el-color-danger)}.el-check-tag.el-check-tag--danger.is-checked:hover{background-color:var(--el-color-danger-light-7)}.el-check-tag.el-check-tag--danger.is-checked.is-disabled{background-color:var(--el-color-danger-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-checked.is-disabled:hover{background-color:var(--el-color-danger-light-8)}.el-check-tag.el-check-tag--danger.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-disabled,.el-check-tag.el-check-tag--danger.is-disabled:hover{background-color:var(--el-color-danger-light-9)}.el-check-tag.el-check-tag--error.is-checked{background-color:var(--el-color-error-light-8);color:var(--el-color-error)}.el-check-tag.el-check-tag--error.is-checked:hover{background-color:var(--el-color-error-light-7)}.el-check-tag.el-check-tag--error.is-checked.is-disabled{background-color:var(--el-color-error-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-checked.is-disabled:hover{background-color:var(--el-color-error-light-8)}.el-check-tag.el-check-tag--error.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-disabled,.el-check-tag.el-check-tag--error.is-disabled:hover{background-color:var(--el-color-error-light-9)}.el-check-tag.el-check-tag--info.is-checked{background-color:var(--el-color-info-light-8);color:var(--el-color-info)}.el-check-tag.el-check-tag--info.is-checked:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--info.is-checked.is-disabled{background-color:var(--el-color-info-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-checked.is-disabled:hover{background-color:var(--el-color-info-light-8)}.el-check-tag.el-check-tag--info.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-disabled,.el-check-tag.el-check-tag--info.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-checkbox-button{--el-checkbox-button-checked-bg-color:var(--el-color-primary);--el-checkbox-button-checked-text-color:var(--el-color-white);--el-checkbox-button-checked-border-color:var(--el-color-primary);display:inline-block;position:relative}.el-checkbox-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left-color:transparent;bord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15px;position:relative;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-checkbox-button__inner.is-round{padding:8px 15px}.el-checkbox-button__inner:hover{color:var(--el-color-primary)}.el-checkbox-button__inner [class*=el-icon-]{line-height:.9}.el-checkbox-button__inner [class*=el-icon-]+span{margin-left:5px}.el-checkbox-button__original{margin:0;opacity:0;outline:none;position:absolute;z-index:-1}.el-checkbox-button.is-checked .el-checkbox-button__inner{background-color:var(--el-checkbox-button-checked-bg-color);border-color:var(--el-checkbox-button-checked-border-color);box-shadow:-1px 0 0 0 var(--el-color-primary-light-7);color:var(--el-checkbox-button-checked-text-color)}.el-checkbox-button.is-checked:first-child .el-checkbox-button__inner{border-left-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button.is-disabled .el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large .el-checkbox-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-checkbox-button--large .el-checkbox-button__inner.is-round{padding:12px 19px}.el-checkbox-button--small .el-checkbox-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-checkbox-button--small .el-checkbox-button__inner.is-round{padding:5px 11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) .el-checkbox__original:not(:focus-visible){border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__input.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-checked-icon-color);content:"";display:block;height:2px;left:0;position:absolute;right:0;top:5px;transform:scale(.5)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:after{display:none}.el-checkbox__inner{background-color:var(--el-checkbox-bg-color);border:var(--el-checkbox-input-border);border-radius:var(--el-checkbox-border-radius);box-sizing:border-box;display:inline-block;height:var(--el-checkbox-input-height);position:relative;transition:border-color .25s cubic-bezier(.71,-.46,.29,1.46),background-color .25s cubic-bezier(.71,-.46,.29,1.46),outline .25s cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 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54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 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var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid 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var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker .el-picker-panel__content{margin:0}.el-date-range-picker__header{height:28px;position:relative;text-align:center}.el-date-range-picker__header [class*=arrow-left]{float:left}.el-date-range-picker__header [class*=arrow-right]{float:right}.el-date-range-picker__header div{font-size:16px;font-weight:500;margin-right:50px}.el-date-range-picker__content{box-sizing:border-box;float:left;margin:0;padding:16px;width:50%}.el-date-range-picker__content.is-left{border-right:1px solid var(--el-datepicker-inner-border-color)}.el-date-range-picker__content .el-date-range-picker__header div{margin-left:50px;margin-right:50px}.el-date-range-picker__editors-wrap{box-sizing:border-box;display:table-cell}.el-date-range-picker__editors-wrap.is-right{text-align:right}.el-date-range-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid var(--el-timepicker-inner-border-color,var(--el-border-color-light));box-sizing:border-box;height:36px;line-height:25px;padding:4px;text-align:right}.el-time-panel__btn{background-color:transparent;border:none;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:28px;margin:0 5px;outline:none;padding:0 5px}.el-time-panel__btn.confirm{color:var(--el-timepicker-active-color,var(--el-color-primary));font-weight:800}.el-descriptions{--el-descriptions-table-border:1px solid var(--el-border-color-lighter);--el-descriptions-item-bordered-label-background:var(--el-fill-color-light);box-sizing:border-box;color:var(--el-text-color-primary);font-size:var(--el-font-size-base)}.el-descriptions__header{align-items:center;display:flex;justify-content:space-between;margin-bottom:16px}.el-descriptions__title{color:var(--el-text-color-primary);font-size:16px;font-weight:700}.el-descriptions__body{background-color:var(--el-fill-color-blank)}.el-descriptions__body 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var(--el-menu-base-level-padding)}.horizontal-collapse-transition .el-sub-menu__title 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.el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small .el-radio__inner{height:12px;width:12px}.el-rate{--el-rate-height:20px;--el-rate-font-size:var(--el-font-size-base);--el-rate-icon-size:18px;--el-rate-icon-margin:6px;--el-rate-void-color:var(--el-border-color-darker);--el-rate-fill-color:#f7ba2a;--el-rate-disabled-void-color:var(--el-fill-color);--el-rate-text-color:var(--el-text-color-primary);align-items:center;display:inline-flex;height:32px}.el-rate:active,.el-rate:focus{outline:none}.el-rate__item{color:var(--el-rate-void-color);cursor:pointer;display:inline-block;font-size:0;line-height:normal;position:relative;vertical-align:middle}.el-rate .el-rate__icon{display:inline-block;font-size:var(--el-rate-icon-size);margin-right:var(--el-rate-icon-margin);position:relative;transition:var(--el-transition-duration)}.el-rate .el-rate__icon.hover{transform:scale(1.15)}.el-rate .el-rate__icon .path2{left:0;position:absolute;top:0}.el-rate .el-rate__icon.is-active{color:var(--el-rate-fill-color)}.el-rate__decimal{color:var(--el-rate-fill-color);display:inline-block;overflow:hidden}.el-rate__decimal,.el-rate__decimal--box{left:0;position:absolute;top:0}.el-rate__text{color:var(--el-rate-text-color);font-size:var(--el-rate-font-size);vertical-align:middle}.el-rate--large{height:40px}.el-rate--small{height:24px}.el-rate--small .el-rate__icon{font-size:14px}.el-rate.is-disabled .el-rate__item{color:var(--el-rate-disabled-void-color);cursor:auto}.el-result{--el-result-padding:40px 30px;--el-result-icon-font-size:64px;--el-result-title-font-size:20px;--el-result-title-margin-top:20px;--el-result-subtitle-margin-top:10px;--el-result-extra-margin-top:30px;align-items:center;box-sizing:border-box;display:flex;flex-direction:column;justify-content:center;padding:var(--el-result-padding);text-align:center}.el-result__icon svg{height:var(--el-result-icon-font-size);width:var(--el-result-icon-font-size)}.el-result__title{margin-top:var(--el-result-title-margin-top)}.el-result__title p{color:var(--el-text-color-primary);font-size:var(--el-result-title-font-size);line-height:1.3;margin:0}.el-result__subtitle{margin-top:var(--el-result-subtitle-margin-top)}.el-result__subtitle p{color:var(--el-text-color-regular);font-size:var(--el-font-size-base);line-height:1.3;margin:0}.el-result__extra{margin-top:var(--el-result-extra-margin-top)}.el-result .icon-primary{--el-result-color:var(--el-color-primary);color:var(--el-result-color)}.el-result .icon-success{--el-result-color:var(--el-color-success);color:var(--el-result-color)}.el-result .icon-warning{--el-result-color:var(--el-color-warning);color:var(--el-result-color)}.el-result .icon-danger{--el-result-color:var(--el-color-danger);color:var(--el-result-color)}.el-result .icon-error{--el-result-color:var(--el-color-error);color:var(--el-result-color)}.el-result 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.el-tag__close{color:var(--el-tag-text-color);flex-shrink:0}.el-tag .el-tag__close:hover{background-color:var(--el-tag-hover-color);color:var(--el-color-white)}.el-tag.el-tag--primary{--el-tag-text-color:var(--el-color-primary)}.el-tag.el-tag--success{--el-tag-text-color:var(--el-color-success)}.el-tag.el-tag--warning{--el-tag-text-color:var(--el-color-warning)}.el-tag.el-tag--danger{--el-tag-text-color:var(--el-color-danger)}.el-tag.el-tag--error{--el-tag-text-color:var(--el-color-error)}.el-tag.el-tag--info{--el-tag-text-color:var(--el-color-info)}.el-tag .el-icon{border-radius:50%;cursor:pointer;font-size:calc(var(--el-icon-size) - 2px);height:var(--el-icon-size);width:var(--el-icon-size)}.el-tag 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0;min-width:0}.time-select .el-picker-panel__content{margin:0;max-height:200px}.time-select-item{font-size:14px;line-height:20px;padding:8px 10px}.time-select-item.disabled{color:var(--el-datepicker-border-color);cursor:not-allowed}.time-select-item:hover{background-color:var(--el-fill-color-light);cursor:pointer;font-weight:700}.time-select .time-select-item.selected:not(.disabled){color:var(--el-color-primary);font-weight:700}.el-timeline-item{padding-bottom:20px;position:relative}.el-timeline-item__wrapper{padding-left:28px;position:relative;top:-3px}.el-timeline-item__tail{border-left:2px solid var(--el-timeline-node-color);height:100%;left:4px;position:absolute}.el-timeline-item .el-timeline-item__icon{color:var(--el-color-white);font-size:var(--el-font-size-small)}.el-timeline-item__node{align-items:center;background-color:var(--el-timeline-node-color);border-color:var(--el-timeline-node-color);border-radius:50%;box-sizing:border-box;display:flex;justify-content:center;position:absolute}.el-timeline-item__node--normal{height:var(--el-timeline-node-size-normal);left:-1px;width:var(--el-timeline-node-size-normal)}.el-timeline-item__node--large{height:var(--el-timeline-node-size-large);left:-2px;width:var(--el-timeline-node-size-large)}.el-timeline-item__node.is-hollow{background:var(--el-color-white);border-style:solid;border-width:2px}.el-timeline-item__node--primary{background-color:var(--el-color-primary);border-color:var(--el-color-primary)}.el-timeline-item__node--success{background-color:var(--el-color-success);border-color:var(--el-color-success)}.el-timeline-item__node--warning{background-color:var(--el-color-warning);border-color:var(--el-color-warning)}.el-timeline-item__node--danger{background-color:var(--el-color-danger);border-color:var(--el-color-danger)}.el-timeline-item__node--info{background-color:var(--el-color-info);border-color:var(--el-color-info)}.el-timeline-item__dot{align-items:center;display:flex;justify-content:center;position:absolute}.el-timeline-item__content{color:var(--el-text-color-primary)}.el-timeline-item__timestamp{color:var(--el-text-color-secondary);font-size:var(--el-font-size-small);line-height:1}.el-timeline-item__timestamp.is-top{margin-bottom:8px;padding-top:4px}.el-timeline-item__timestamp.is-bottom{margin-top:8px}.el-timeline{--el-timeline-node-size-normal:12px;--el-timeline-node-size-large:14px;--el-timeline-node-color:var(--el-border-color-light);font-size:var(--el-font-size-base);list-style:none;margin:0}.el-timeline 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router-link-exact-active mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/attributions/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":11,"prerenderedAt":12},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{},"/attributions",1727871092425]</script>
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diff --git a/.output/public/contribution/_payload.json b/.output/public/contribution/_payload.json
index 89ba30e867d336d431f3d11fb4cf8afc72ea1dd4..6bc13c5ad4cd014a647052dc1d1119290d72edbe 100644
--- a/.output/public/contribution/_payload.json
+++ b/.output/public/contribution/_payload.json
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\ No newline at end of file
+[{"data":1,"prerenderedAt":3},["Reactive",2],{},1727871092436]
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diff --git a/.output/public/contribution/index.html b/.output/public/contribution/index.html
index 3550fa02be3770a12c99947c498a599c3285a010..34e3c6ddf35a52ae2f22d9cb863f5e5b3e92aa55 100644
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20px;position:relative}.el-cascader-node.is-selectable.in-active-path{color:var(--el-cascader-menu-text-color)}.el-cascader-node.in-active-path,.el-cascader-node.is-active,.el-cascader-node.is-selectable.in-checked-path{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader-node:not(.is-disabled){cursor:pointer}.el-cascader-node:not(.is-disabled):focus,.el-cascader-node:not(.is-disabled):hover{background:var(--el-cascader-node-background-hover)}.el-cascader-node.is-disabled{color:var(--el-cascader-node-color-disabled);cursor:not-allowed}.el-cascader-node__prefix{left:10px;position:absolute}.el-cascader-node__postfix{position:absolute;right:10px}.el-cascader-node__label{flex:1;overflow:hidden;padding:0 8px;text-align:left;text-overflow:ellipsis;white-space:nowrap}.el-cascader-node>.el-checkbox,.el-cascader-node>.el-radio{margin-right:0}.el-cascader-node>.el-radio .el-radio__label{padding-left:0}.el-cascader{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);display:inline-block;font-size:var(--el-font-size-base);line-height:32px;outline:none;position:relative;vertical-align:middle}.el-cascader:not(.is-disabled):hover .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset;cursor:pointer}.el-cascader .el-input{cursor:pointer;display:flex}.el-cascader .el-input .el-input__inner{cursor:pointer;text-overflow:ellipsis}.el-cascader .el-input .el-input__suffix-inner .el-icon{height:calc(100% - 2px)}.el-cascader .el-input .el-input__suffix-inner .el-icon svg{vertical-align:middle}.el-cascader .el-input .icon-arrow-down{font-size:14px;transition:transform var(--el-transition-duration)}.el-cascader .el-input .icon-arrow-down.is-reverse{transform:rotate(180deg)}.el-cascader .el-input .icon-circle-close:hover{color:var(--el-input-clear-hover-color,var(--el-text-color-secondary))}.el-cascader .el-input.is-focus .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-focus-border-color,var(--el-color-primary)) inset}.el-cascader--large{font-size:14px;line-height:40px}.el-cascader--small{font-size:12px;line-height:24px}.el-cascader.is-disabled .el-cascader__label{color:var(--el-disabled-text-color);z-index:calc(var(--el-index-normal) + 1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper .el-popper__arrow:before{border:var(--el-cascader-menu-border)}.el-cascader__dropdown.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper{box-shadow:var(--el-cascader-menu-shadow)}.el-cascader__tags{box-sizing:border-box;display:flex;flex-wrap:wrap;left:0;line-height:normal;position:absolute;right:30px;text-align:left;top:50%;transform:translateY(-50%)}.el-cascader__tags .el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags .el-tag{align-items:center;background:var(--el-fill-color);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag.el-tag--dark,.el-cascader__collapse-tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__collapse-tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__collapse-tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__collapse-tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__suggestion-panel{border-radius:var(--el-cascader-menu-radius)}.el-cascader__suggestion-list{color:var(--el-cascader-menu-text-color);font-size:var(--el-font-size-base);margin:0;max-height:204px;padding:6px 0;text-align:center}.el-cascader__suggestion-item{align-items:center;cursor:pointer;display:flex;height:34px;justify-content:space-between;outline:none;padding:0 15px;text-align:left}.el-cascader__suggestion-item:focus,.el-cascader__suggestion-item:hover{background:var(--el-cascader-node-background-hover)}.el-cascader__suggestion-item.is-checked{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader__suggestion-item>span{margin-right:10px}.el-cascader__empty-text{color:var(--el-cascader-color-empty);margin:10px 0}.el-cascader__search-input{background:transparent;border:none;box-sizing:border-box;color:var(--el-cascader-menu-text-color);flex:1;height:24px;margin:2px 0 2px 11px;min-width:60px;outline:none;padding:0}.el-cascader__search-input::-moz-placeholder{color:transparent}.el-cascader__search-input::placeholder{color:transparent}.el-check-tag{background-color:var(--el-color-info-light-9);border-radius:var(--el-border-radius-base);color:var(--el-color-info);cursor:pointer;display:inline-block;font-size:var(--el-font-size-base);font-weight:700;line-height:var(--el-font-size-base);padding:7px 15px;transition:var(--el-transition-all)}.el-check-tag:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--primary.is-checked{background-color:var(--el-color-primary-light-8);color:var(--el-color-primary)}.el-check-tag.el-check-tag--primary.is-checked:hover{background-color:var(--el-color-primary-light-7)}.el-check-tag.el-check-tag--primary.is-checked.is-disabled{background-color:var(--el-color-primary-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-checked.is-disabled:hover{background-color:var(--el-color-primary-light-8)}.el-check-tag.el-check-tag--primary.is-disabled{background-color:var(--el-color-info-light-9);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-check-tag.el-check-tag--success.is-checked{background-color:var(--el-color-success-light-8);color:var(--el-color-success)}.el-check-tag.el-check-tag--success.is-checked:hover{background-color:var(--el-color-success-light-7)}.el-check-tag.el-check-tag--success.is-checked.is-disabled{background-color:var(--el-color-success-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-checked.is-disabled:hover{background-color:var(--el-color-success-light-8)}.el-check-tag.el-check-tag--success.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-disabled,.el-check-tag.el-check-tag--success.is-disabled:hover{background-color:var(--el-color-success-light-9)}.el-check-tag.el-check-tag--warning.is-checked{background-color:var(--el-color-warning-light-8);color:var(--el-color-warning)}.el-check-tag.el-check-tag--warning.is-checked:hover{background-color:var(--el-color-warning-light-7)}.el-check-tag.el-check-tag--warning.is-checked.is-disabled{background-color:var(--el-color-warning-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-checked.is-disabled:hover{background-color:var(--el-color-warning-light-8)}.el-check-tag.el-check-tag--warning.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-disabled,.el-check-tag.el-check-tag--warning.is-disabled:hover{background-color:var(--el-color-warning-light-9)}.el-check-tag.el-check-tag--danger.is-checked{background-color:var(--el-color-danger-light-8);color:var(--el-color-danger)}.el-check-tag.el-check-tag--danger.is-checked:hover{background-color:var(--el-color-danger-light-7)}.el-check-tag.el-check-tag--danger.is-checked.is-disabled{background-color:var(--el-color-danger-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-checked.is-disabled:hover{background-color:var(--el-color-danger-light-8)}.el-check-tag.el-check-tag--danger.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-disabled,.el-check-tag.el-check-tag--danger.is-disabled:hover{background-color:var(--el-color-danger-light-9)}.el-check-tag.el-check-tag--error.is-checked{background-color:var(--el-color-error-light-8);color:var(--el-color-error)}.el-check-tag.el-check-tag--error.is-checked:hover{background-color:var(--el-color-error-light-7)}.el-check-tag.el-check-tag--error.is-checked.is-disabled{background-color:var(--el-color-error-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-checked.is-disabled:hover{background-color:var(--el-color-error-light-8)}.el-check-tag.el-check-tag--error.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-disabled,.el-check-tag.el-check-tag--error.is-disabled:hover{background-color:var(--el-color-error-light-9)}.el-check-tag.el-check-tag--info.is-checked{background-color:var(--el-color-info-light-8);color:var(--el-color-info)}.el-check-tag.el-check-tag--info.is-checked:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--info.is-checked.is-disabled{background-color:var(--el-color-info-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-checked.is-disabled:hover{background-color:var(--el-color-info-light-8)}.el-check-tag.el-check-tag--info.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-disabled,.el-check-tag.el-check-tag--info.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-checkbox-button{--el-checkbox-button-checked-bg-color:var(--el-color-primary);--el-checkbox-button-checked-text-color:var(--el-color-white);--el-checkbox-button-checked-border-color:var(--el-color-primary);display:inline-block;position:relative}.el-checkbox-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left-color:transparent;bord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15px;position:relative;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-checkbox-button__inner.is-round{padding:8px 15px}.el-checkbox-button__inner:hover{color:var(--el-color-primary)}.el-checkbox-button__inner [class*=el-icon-]{line-height:.9}.el-checkbox-button__inner [class*=el-icon-]+span{margin-left:5px}.el-checkbox-button__original{margin:0;opacity:0;outline:none;position:absolute;z-index:-1}.el-checkbox-button.is-checked .el-checkbox-button__inner{background-color:var(--el-checkbox-button-checked-bg-color);border-color:var(--el-checkbox-button-checked-border-color);box-shadow:-1px 0 0 0 var(--el-color-primary-light-7);color:var(--el-checkbox-button-checked-text-color)}.el-checkbox-button.is-checked:first-child .el-checkbox-button__inner{border-left-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button.is-disabled .el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large .el-checkbox-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-checkbox-button--large .el-checkbox-button__inner.is-round{padding:12px 19px}.el-checkbox-button--small .el-checkbox-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-checkbox-button--small .el-checkbox-button__inner.is-round{padding:5px 11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) .el-checkbox__original:not(:focus-visible){border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__input.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-checked-icon-color);content:"";display:block;height:2px;left:0;position:absolute;right:0;top:5px;transform:scale(.5)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:after{display:none}.el-checkbox__inner{background-color:var(--el-checkbox-bg-color);border:var(--el-checkbox-input-border);border-radius:var(--el-checkbox-border-radius);box-sizing:border-box;display:inline-block;height:var(--el-checkbox-input-height);position:relative;transition:border-color .25s cubic-bezier(.71,-.46,.29,1.46),background-color .25s cubic-bezier(.71,-.46,.29,1.46),outline .25s cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 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54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 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var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid 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var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker .el-picker-panel__content{margin:0}.el-date-range-picker__header{height:28px;position:relative;text-align:center}.el-date-range-picker__header [class*=arrow-left]{float:left}.el-date-range-picker__header [class*=arrow-right]{float:right}.el-date-range-picker__header div{font-size:16px;font-weight:500;margin-right:50px}.el-date-range-picker__content{box-sizing:border-box;float:left;margin:0;padding:16px;width:50%}.el-date-range-picker__content.is-left{border-right:1px solid var(--el-datepicker-inner-border-color)}.el-date-range-picker__content .el-date-range-picker__header div{margin-left:50px;margin-right:50px}.el-date-range-picker__editors-wrap{box-sizing:border-box;display:table-cell}.el-date-range-picker__editors-wrap.is-right{text-align:right}.el-date-range-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid var(--el-timepicker-inner-border-color,var(--el-border-color-light));box-sizing:border-box;height:36px;line-height:25px;padding:4px;text-align:right}.el-time-panel__btn{background-color:transparent;border:none;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:28px;margin:0 5px;outline:none;padding:0 5px}.el-time-panel__btn.confirm{color:var(--el-timepicker-active-color,var(--el-color-primary));font-weight:800}.el-descriptions{--el-descriptions-table-border:1px solid var(--el-border-color-lighter);--el-descriptions-item-bordered-label-background:var(--el-fill-color-light);box-sizing:border-box;color:var(--el-text-color-primary);font-size:var(--el-font-size-base)}.el-descriptions__header{align-items:center;display:flex;justify-content:space-between;margin-bottom:16px}.el-descriptions__title{color:var(--el-text-color-primary);font-size:16px;font-weight:700}.el-descriptions__body{background-color:var(--el-fill-color-blank)}.el-descriptions__body 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var(--el-menu-base-level-padding)}.horizontal-collapse-transition .el-sub-menu__title 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.el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small .el-radio__inner{height:12px;width:12px}.el-rate{--el-rate-height:20px;--el-rate-font-size:var(--el-font-size-base);--el-rate-icon-size:18px;--el-rate-icon-margin:6px;--el-rate-void-color:var(--el-border-color-darker);--el-rate-fill-color:#f7ba2a;--el-rate-disabled-void-color:var(--el-fill-color);--el-rate-text-color:var(--el-text-color-primary);align-items:center;display:inline-flex;height:32px}.el-rate:active,.el-rate:focus{outline:none}.el-rate__item{color:var(--el-rate-void-color);cursor:pointer;display:inline-block;font-size:0;line-height:normal;position:relative;vertical-align:middle}.el-rate .el-rate__icon{display:inline-block;font-size:var(--el-rate-icon-size);margin-right:var(--el-rate-icon-margin);position:relative;transition:var(--el-transition-duration)}.el-rate .el-rate__icon.hover{transform:scale(1.15)}.el-rate .el-rate__icon .path2{left:0;position:absolute;top:0}.el-rate .el-rate__icon.is-active{color:var(--el-rate-fill-color)}.el-rate__decimal{color:var(--el-rate-fill-color);display:inline-block;overflow:hidden}.el-rate__decimal,.el-rate__decimal--box{left:0;position:absolute;top:0}.el-rate__text{color:var(--el-rate-text-color);font-size:var(--el-rate-font-size);vertical-align:middle}.el-rate--large{height:40px}.el-rate--small{height:24px}.el-rate--small .el-rate__icon{font-size:14px}.el-rate.is-disabled .el-rate__item{color:var(--el-rate-disabled-void-color);cursor:auto}.el-result{--el-result-padding:40px 30px;--el-result-icon-font-size:64px;--el-result-title-font-size:20px;--el-result-title-margin-top:20px;--el-result-subtitle-margin-top:10px;--el-result-extra-margin-top:30px;align-items:center;box-sizing:border-box;display:flex;flex-direction:column;justify-content:center;padding:var(--el-result-padding);text-align:center}.el-result__icon svg{height:var(--el-result-icon-font-size);width:var(--el-result-icon-font-size)}.el-result__title{margin-top:var(--el-result-title-margin-top)}.el-result__title p{color:var(--el-text-color-primary);font-size:var(--el-result-title-font-size);line-height:1.3;margin:0}.el-result__subtitle{margin-top:var(--el-result-subtitle-margin-top)}.el-result__subtitle p{color:var(--el-text-color-regular);font-size:var(--el-font-size-base);line-height:1.3;margin:0}.el-result__extra{margin-top:var(--el-result-extra-margin-top)}.el-result .icon-primary{--el-result-color:var(--el-color-primary);color:var(--el-result-color)}.el-result .icon-success{--el-result-color:var(--el-color-success);color:var(--el-result-color)}.el-result .icon-warning{--el-result-color:var(--el-color-warning);color:var(--el-result-color)}.el-result .icon-danger{--el-result-color:var(--el-color-danger);color:var(--el-result-color)}.el-result .icon-error{--el-result-color:var(--el-color-error);color:var(--el-result-color)}.el-result 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.el-tag__close{color:var(--el-tag-text-color);flex-shrink:0}.el-tag .el-tag__close:hover{background-color:var(--el-tag-hover-color);color:var(--el-color-white)}.el-tag.el-tag--primary{--el-tag-text-color:var(--el-color-primary)}.el-tag.el-tag--success{--el-tag-text-color:var(--el-color-success)}.el-tag.el-tag--warning{--el-tag-text-color:var(--el-color-warning)}.el-tag.el-tag--danger{--el-tag-text-color:var(--el-color-danger)}.el-tag.el-tag--error{--el-tag-text-color:var(--el-color-error)}.el-tag.el-tag--info{--el-tag-text-color:var(--el-color-info)}.el-tag .el-icon{border-radius:50%;cursor:pointer;font-size:calc(var(--el-icon-size) - 2px);height:var(--el-icon-size);width:var(--el-icon-size)}.el-tag 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0;min-width:0}.time-select .el-picker-panel__content{margin:0;max-height:200px}.time-select-item{font-size:14px;line-height:20px;padding:8px 10px}.time-select-item.disabled{color:var(--el-datepicker-border-color);cursor:not-allowed}.time-select-item:hover{background-color:var(--el-fill-color-light);cursor:pointer;font-weight:700}.time-select .time-select-item.selected:not(.disabled){color:var(--el-color-primary);font-weight:700}.el-timeline-item{padding-bottom:20px;position:relative}.el-timeline-item__wrapper{padding-left:28px;position:relative;top:-3px}.el-timeline-item__tail{border-left:2px solid var(--el-timeline-node-color);height:100%;left:4px;position:absolute}.el-timeline-item .el-timeline-item__icon{color:var(--el-color-white);font-size:var(--el-font-size-small)}.el-timeline-item__node{align-items:center;background-color:var(--el-timeline-node-color);border-color:var(--el-timeline-node-color);border-radius:50%;box-sizing:border-box;display:flex;justify-content:center;position:absolute}.el-timeline-item__node--normal{height:var(--el-timeline-node-size-normal);left:-1px;width:var(--el-timeline-node-size-normal)}.el-timeline-item__node--large{height:var(--el-timeline-node-size-large);left:-2px;width:var(--el-timeline-node-size-large)}.el-timeline-item__node.is-hollow{background:var(--el-color-white);border-style:solid;border-width:2px}.el-timeline-item__node--primary{background-color:var(--el-color-primary);border-color:var(--el-color-primary)}.el-timeline-item__node--success{background-color:var(--el-color-success);border-color:var(--el-color-success)}.el-timeline-item__node--warning{background-color:var(--el-color-warning);border-color:var(--el-color-warning)}.el-timeline-item__node--danger{background-color:var(--el-color-danger);border-color:var(--el-color-danger)}.el-timeline-item__node--info{background-color:var(--el-color-info);border-color:var(--el-color-info)}.el-timeline-item__dot{align-items:center;display:flex;justify-content:center;position:absolute}.el-timeline-item__content{color:var(--el-text-color-primary)}.el-timeline-item__timestamp{color:var(--el-text-color-secondary);font-size:var(--el-font-size-small);line-height:1}.el-timeline-item__timestamp.is-top{margin-bottom:8px;padding-top:4px}.el-timeline-item__timestamp.is-bottom{margin-top:8px}.el-timeline{--el-timeline-node-size-normal:12px;--el-timeline-node-size-large:14px;--el-timeline-node-color:var(--el-border-color-light);font-size:var(--el-font-size-base);list-style:none;margin:0}.el-timeline 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hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/contribution/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":11,"prerenderedAt":12},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{},"/contribution",1727871092436]</script>
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diff --git a/.output/public/description/_payload.json b/.output/public/description/_payload.json
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--- a/.output/public/description/_payload.json
+++ b/.output/public/description/_payload.json
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-[{"data":1,"prerenderedAt":328},["Reactive",2],{"content-query-Pcus8P4khp":3},{"_path":4,"_dir":5,"_draft":6,"_partial":6,"_locale":5,"title":7,"description":5,"body":8,"_type":322,"_id":323,"_source":324,"_file":325,"_stem":326,"_extension":327},"/project-description","",false,"Description",{"type":9,"children":10,"toc":306},"root",[11,20,26,31,36,41,45,50,56,61,66,71,76,82,88,93,98,111,116,121,126,131,136,142,147,153,158,163,171,178,185,192,197,202,209,218],{"type":12,"tag":13,"props":14,"children":16},"element","h2",{"id":15},"background",[17],{"type":18,"value":19},"text","Background",{"type":12,"tag":21,"props":22,"children":23},"p",{},[24],{"type":18,"value":25},"Environmental endocrine disruptors (EDCs) are a class of chemical substances that can mimic or block the effects of hormones, affecting the normal development of organisms. These substances include DDT, dioxins, and phthalates, among others.",{"type":12,"tag":21,"props":27,"children":28},{},[29],{"type":18,"value":30},"...",{"type":12,"tag":21,"props":32,"children":33},{},[34],{"type":18,"value":35},"DDT is an insecticide that was once widely used, but due to its harmful effects on the reproductive and nervous systems of organisms, as well as its potential carcinogenicity, its use has been restricted in many countries since the 1970s and 1980s. However, as these substances have already entered the environment in large quantities, their negative effects persist even though their production has stopped for many years. They accumulate in the atmosphere, soil, water bodies, and organisms, posing a natural hazard.",{"type":12,"tag":21,"props":37,"children":38},{},[39],{"type":18,"value":40},"In 2010, the direct social and economic loss caused by exposure to phthalate chemicals in China reached approximately 57.2 billion yuan, with the burden of male infertility being the most severe. Further calculations indicate that the total medical cost for all known EDCs exposure in China is about 429.4 billion yuan, accounting for approximately 1.07% of the annual GDP.",{"type":12,"tag":21,"props":42,"children":43},{},[44],{"type":18,"value":30},{"type":12,"tag":21,"props":46,"children":47},{},[48],{"type":18,"value":49},"All these indicate that we must pay attention to these potential dangers and have a more comprehensive understanding and early screening of environmental endocrine disruptors. Nowadays, with the deepening research on environmental endocrine disruptors, more and more studies are beginning to focus on the relationship between endocrine disruptors and their metabolites and other toxic effects. This superposition of multiple effects provides clues to revealing the molecular mechanisms underlying many diseases.",{"type":12,"tag":13,"props":51,"children":53},{"id":52},"problem",[54],{"type":18,"value":55},"Problem",{"type":12,"tag":21,"props":57,"children":58},{},[59],{"type":18,"value":60},"In fact, the role of environmental endocrine disruptors in organisms is complex, and the inference of their adverse effects needs to emphasize the adverse outcome pathway (AOP pathway, which describes the logical derivation of causally related events at different levels of biological tissues, leading to adverse effects on human or wildlife health). This requires us to describe the molecular mechanisms behind the impact comprehensively.",{"type":12,"tag":21,"props":62,"children":63},{},[64],{"type":18,"value":65},"Currently, the methods for detecting endocrine disruptors using biological effects mainly include competitive ligand binding assays, fused cell aggregation experiments, and animal cell proliferation analysis experiments. These methods can only evaluate the endocrine disruption effect through the binding effect of hormone receptors when analyzing endocrine disruptors, and cannot judge the involvement of other effects behind the biological damage caused by endocrine disruptors, therefore, they have limitations.",{"type":12,"tag":21,"props":67,"children":68},{},[69],{"type":18,"value":70},"More and more literature has proven the important role of oxidative damage effects in the damage caused by environmental endocrine disruptors to organisms. For example, exposure to various parabens (PBs) and their metabolites can increase the level of oxidative stress, and the superposition of oxidative damage and endocrine disruption increases the risk of impaired reproductive capacity in organisms. The collaborative analysis of environmental endocrine disruption effects and oxidative damage effects can help us predict the pathways of action of environmental endocrine disruptors on organism damage, thereby assisting us in having a clearer understanding of their damage consequences and providing a reference for regulatory authorities to implement targeted regulatory policies.",{"type":12,"tag":21,"props":72,"children":73},{},[74],{"type":18,"value":75},"Our project is based on this concept and aims to construct microbial sensors through synthetic biology methods to provide multiple detection lines for the assessment of endocrine disruption effects and oxidative damage effects. Not only can we achieve the separate detection of the two effects, but we can also realize the collaborative analysis of the two effects through logic gate detection lines, achieving a rapid, convenient, and comprehensive assessment of environmental endocrine disruptors. Conduct more in-depth research and discussion on the impact mechanism of environmental endocrine disruptors.",{"type":12,"tag":13,"props":77,"children":79},{"id":78},"inspiration",[80],{"type":18,"value":81},"Inspiration",{"type":12,"tag":83,"props":84,"children":86},"h3",{"id":85},"biology",[87],{"type":18,"value":85},{"type":12,"tag":21,"props":89,"children":90},{},[91],{"type":18,"value":92},"Initially, we planned to develop a biosensor for detecting environmental endocrine disruptors. To achieve this goal, we studied the characteristics and mechanisms of action of environmental endocrine disruptors and researched the methods for detecting them. Intein peptides are protein analogs found in self-splicing RNA introns, and their insertion can lead to the inactivation of the host protein. Typically, they excise themselves from the host cell after translation, restoring the activity of the host protein. By replacing the homing endonuclease domain in the intein peptide with a hormone receptor binding domain, we can construct a hormone-sensitive intein peptide, which can be used to create recognition elements for endocrine disruption effects. We plan to construct an estrogen-sensitive intein peptide and insert it into T7 RNA Polymerase. T7 RNA Polymerase can specifically activate the T7 promoter, thus regulating the downstream circuit to express the reporter gene upon detection of estrogen mimics through the T7 promoter.",{"type":12,"tag":21,"props":94,"children":95},{},[96],{"type":18,"value":97},"Subsequently, through more in-depth researches, it was found that the effects of environmental endocrine disruptors in organisms are complex. An increasing number of studies have demonstrated the significant role of oxidative stress in the damage caused by environmental endocrine disruptors. For example, exposure to various paraben compounds (PBs) and their metabolites can increase oxidative stress levels. The combined oxidative damage and endocrine disruption can heighten the risk of impaired reproductive capability in organisms. A synergistic analysis of the effects of environmental endocrine disruption and oxidative damage can help us predict the pathways through which these disruptors cause harm to organisms, thereby assisting us in gaining a clearer understanding of their damaging consequences. Therefore, we plan to incorporate DNA damage detection, designing an and gate detection circuit capable of simultaneously detecting endocrine disruption effects and DNA oxidative damage effects.",{"type":12,"tag":21,"props":99,"children":100},{},[101,103,109],{"type":18,"value":102},"DNA damage detection is based on the SOS response mechanism in ",{"type":12,"tag":104,"props":105,"children":106},"em",{},[107],{"type":18,"value":108},"Escherichia coli",{"type":18,"value":110},", which is an inducible DNA repair system activated when bacterial DNA is damaged. When bacteria detect DNA damage, the expression levels of genes associated with the SOS response mechanism significantly increase, leading to the expression of proteases that relieve the repression of target proteins, initiating the repair process. We chose the recA promoter to detect DNA damage, when bacteria sense DNA damage, the expression level of this promoter rises significantly, thereby activating downstream reporter genes.",{"type":12,"tag":21,"props":112,"children":113},{},[114],{"type":18,"value":115},"To construct the logic gate, we introduced the hrp system, which consists of the hrpR and hrpS genes along with the HrpL promoter regulated by the sigma54 factor. The hrpL promoter is activated only when both hrpR and hrpS genes are expressed simultaneously.",{"type":12,"tag":21,"props":117,"children":118},{},[119],{"type":18,"value":120},"We plan to insert the hrpR gene downstream of the P recA promoter, which will be activated in response to DNA damage effects; the hrpS gene will be inserted downstream of the P T7 promoter, which will be activated after the peptide-specific binding domain binds to estrogen analogs. The reporter gene egfp will be inserted downstream of the P hrpL promoter, resulting in a two-input AND gate circuit.",{"type":12,"tag":21,"props":122,"children":123},{},[124],{"type":18,"value":125},"However, the entire circuit is too large, posing significant challenges to the stability of the plasmid and the burden on the engineered bacteria. Therefore, we plan to divide the entire circuit into two parts, each constructed in different engineered bacteria, inserting new components to enable signal transmission between the two engineered bacterias.",{"type":12,"tag":21,"props":127,"children":128},{},[129],{"type":18,"value":130},"Throughout the system, we have always planned to use the egfp gene as the reporter gene. However, this gene requires the presence of oxygen molecules as a cofactor for its luminescence, while the cultivation chamber of our designed hardware is in a low-oxygen environment. As a result, we tested a new reporter gene, BsFbFp, which can express significantly enhanced in vivo fluorescence in both aerobic and anaerobic environments, ensuring that the engineered bacteria can effectively express fluorescent signals in low-oxygen conditions.",{"type":12,"tag":21,"props":132,"children":133},{},[134],{"type":18,"value":135},"Thus, the construction of the biological part of our circuit has been completed.",{"type":12,"tag":83,"props":137,"children":139},{"id":138},"hardware",[140],{"type":18,"value":141},"Hardware",{"type":12,"tag":21,"props":143,"children":144},{},[145],{"type":18,"value":146},"Point of Care Testing (POCT) is a form of rapid on-site testing/near-patient testing method which has been particularly impactful and is playing an increasingly vital role in the fields of medical diagnostics and environmental health monitoring due to its speed, convenience, and ease of operation. Traditional water contaminant detection technologies are often constrained by the need for costly equipment and a professional laboratory environment. In contrast, novel biosensor detection devices based on compact POCT equipment, when connected to an interactive app for operational control and visual data analysis, can effectively facilitate the rapid and efficient screening of emerging waterborne pollutants. This approach holds broad prospects for application in the field of environmental monitoring and assessment.",{"type":12,"tag":13,"props":148,"children":150},{"id":149},"solution",[151],{"type":18,"value":152},"Solution",{"type":12,"tag":83,"props":154,"children":156},{"id":155},"biology-1",[157],{"type":18,"value":85},{"type":12,"tag":21,"props":159,"children":160},{},[161],{"type":18,"value":162},"To achieve signal communication between the two engineered bacteria, we studied the bacterial quorum sensing mechanism. This mechanism allows bacteria to exchange information and coordinate population behaviors when their numbers reach a certain density, regulating gene expression. When activated, bacteria secrete signal molecules that can freely diffuse across the cell membrane. Once the concentration of these signal molecules in the environment reaches a certain threshold, it induces the expression of specific genes in the bacteria. We introduced the LuxI/R system, where AHL (N-acyl homoserine lactone) is produced catalytically by the expression of the LuxI gene as a signaling molecule. This AHL freely diffuses into the second engineered bacteria and binds with the LuxR protein released by the activation of the LuxR gene, activating the P LuxR promoter to initiate downstream gene expression.",{"type":12,"tag":21,"props":164,"children":165},{},[166],{"type":12,"tag":167,"props":168,"children":170},"img",{"alt":167,"src":169},"https://static.igem.wiki/teams/5358/description/biocircuit-p1.png",[],{"type":12,"tag":21,"props":172,"children":173},{},[174],{"type":12,"tag":167,"props":175,"children":177},{"alt":167,"src":176},"https://static.igem.wiki/teams/5358/description/biocircuit-p2.png",[],{"type":12,"tag":21,"props":179,"children":180},{},[181],{"type":12,"tag":167,"props":182,"children":184},{"alt":167,"src":183},"https://static.igem.wiki/teams/5358/description/biocircuit-p3.png",[],{"type":12,"tag":21,"props":186,"children":187},{},[188],{"type":12,"tag":167,"props":189,"children":191},{"alt":167,"src":190},"https://static.igem.wiki/teams/5358/description/biocircuit-p4.png",[],{"type":12,"tag":83,"props":193,"children":195},{"id":194},"hardware-1",[196],{"type":18,"value":141},{"type":12,"tag":21,"props":198,"children":199},{},[200],{"type":18,"value":201},"In response to the demands for standardized cultivation of engineered bacteria and high-sensitivity detection, we have designed a highly integrated and automated system that encompasses the entire process of bacterial strain revival, cultivation, detection, and analysis. The system utilizes an ESP32 chip as the main controller, interfaces with a temperature control system and a fluorescence detection system, employs a linear slide rail structure for high-throughput detection, and leverages a mobile app for automated device control and visualization of detection results. 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Disease burden attributable to endocrine-disrupting chemicals exposure in China: A case study of phthalates. Science of the Total Environment, 2019, Vol. 662(1): 615-621",{"type":12,"tag":227,"props":233,"children":234},{},[235],{"type":18,"value":236},"Nematollah Asadi, Mahmoud Bahmani, Arash Kheradmand, Mahmoud Rafieian-Kopaei. The Impact of Oxidative Stress on Testicular Function and the Role of Antioxidants in Improving it: A Review. Journal of Clinical and Diagnostic Research, 2017, Vol. 11(5): IE1-IE5",{"type":12,"tag":227,"props":238,"children":239},{},[240],{"type":18,"value":241},"Ma Ya, Liu Haohao, Wu Jinxia, Yuan Le, Wang Yueqin, Du Xingde, Wang Rui, Marwa Phelisters Wegesa, Petlulu Pavankumar, Chen Xinghai, Zhang Huizhen. The adverse health effects of bisphenol A and related toxicity mechanisms. Environmental Research, 2019, Vol. 176: 108",{"type":12,"tag":227,"props":243,"children":244},{},[245],{"type":18,"value":246},"Liang R , Zhou J , Liu J. Construction of a Bacterial Assay for Estrogen Detection Based on an Estrogen-Sensitive Intein. Applied & Environmental Microbiology, 2011, 77(7):2488.DOI:10.1128/AEM.02336-10.",{"type":12,"tag":227,"props":248,"children":249},{},[250],{"type":18,"value":251},"Ma Ya, Liu Haohao, Wu Jinxia, Yuan Le, Wang Yueqin, Du Xingde, Wang Rui, Marwa, Phelisters Wegesa, Petlulu, Pavankumar, Chen Xinghai, Zhang Huizhen.The adverse health effects of bisphenol A and related toxicity mechanisms.Environmental Research, 2019, Vol.176: 108575",{"type":12,"tag":227,"props":253,"children":254},{},[255,257,262],{"type":18,"value":256},"LIANG Jing-jia, GU Ai-hua. Multigenerational and cross-generational effect of environmental endocrine disruptors on reproductive system in male animals. ",{"type":12,"tag":104,"props":258,"children":259},{},[260],{"type":18,"value":261},"Chinese Journal of Public Health",{"type":18,"value":263},", 2021, 37(2): 375-380.",{"type":12,"tag":227,"props":265,"children":266},{},[267],{"type":18,"value":268},"Nematollah Asadi, Mahmoud Bahmani, Arash Kheradmand, Mahmoud Rafieian-Kopaei.The Impact of Oxidative Stress on Testicular Function and the Role of Antioxidants in Improving it: A Review.Journal of Clinical and Diagnostic Research, 2017, Vol.11(5): IE1-IE5",{"type":12,"tag":227,"props":270,"children":271},{},[272],{"type":18,"value":273},"Mondal Shirsha, Ghosh Songita, Bhattacharya Samir, Mukherjee Sutapa. Chronic dietary administration of lower levels of diethyl phthalate induces murine testicular germ cell inflammation and sperm pathologies: Involvement of oxidative stress. Chemosphere, 2019, Vol.229: 443-451",{"type":12,"tag":227,"props":275,"children":276},{},[277],{"type":18,"value":278},"Maslowska K H, Karolina Makiela‐Dzbenska, Fijalkowska I J. The SOS system: A complex and tightly regulated response to DNA damage. John Wiley & Sons, Ltd, 2019(4). DOI:10.1002/em.22267.",{"type":12,"tag":227,"props":280,"children":281},{},[282],{"type":18,"value":283},"Baojun W, Mauricio B, Martin B. Engineering modular and tunable genetic amplifiers for scaling transcriptional signals in cascaded gene networks. Nucleic Acids Research, 2014(14): 9484-9492. DOI:10.1093/nar/gku593.",{"type":12,"tag":227,"props":285,"children":286},{},[287],{"type":18,"value":288},"Xiao Y, Zou H, Li J, Song T, Lv W, Wang W, Wang Z, Tao S. Impact of quorum sensing signaling molecules in gram-negative bacteria on host cells: current understanding and future perspectives. Gut Microbes. 2022 Jan-Dec, 14(1):2039048. doi: 10.1080/19490976.2022.2039048.",{"type":12,"tag":227,"props":290,"children":291},{},[292],{"type":18,"value":293},"Drepper T, Eggert T, Circolone F, et al. Reporter proteins for in vivo fluorescence without oxygen. Nature Biotechnology, 2007, 25(4):443-445.DOI:10.1038/nbt1293.",{"type":12,"tag":227,"props":295,"children":296},{},[297,299,304],{"type":18,"value":298},"Mukherjee A, Schroeder C M, Sikes H D, et al. Flavin-based fluorescent proteins: emerging paradigms in biological imaging. ",{"type":12,"tag":104,"props":300,"children":301},{},[302],{"type":18,"value":303},"This review comes from a themed issue on Analytical biotechnology",{"type":18,"value":305},". 2015.",{"title":5,"searchDepth":307,"depth":308,"links":309},1,3,[310,312,313,317,321],{"id":15,"depth":311,"text":19},2,{"id":52,"depth":311,"text":55},{"id":78,"depth":311,"text":81,"children":314},[315,316],{"id":85,"depth":308,"text":85},{"id":138,"depth":308,"text":141},{"id":149,"depth":311,"text":152,"children":318},[319,320],{"id":155,"depth":308,"text":85},{"id":194,"depth":308,"text":141},{"id":211,"depth":311,"text":217},"markdown","content:project-description.md","content","project-description.md","project-description","md",1727868995247]
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These substances include DDT, dioxins, and phthalates, among others.",{"type":12,"tag":21,"props":27,"children":28},{},[29],{"type":18,"value":30},"...",{"type":12,"tag":21,"props":32,"children":33},{},[34],{"type":18,"value":35},"DDT is an insecticide that was once widely used, but due to its harmful effects on the reproductive and nervous systems of organisms, as well as its potential carcinogenicity, its use has been restricted in many countries since the 1970s and 1980s. However, as these substances have already entered the environment in large quantities, their negative effects persist even though their production has stopped for many years. They accumulate in the atmosphere, soil, water bodies, and organisms, posing a natural hazard.",{"type":12,"tag":21,"props":37,"children":38},{},[39],{"type":18,"value":40},"In 2010, the direct social and economic loss caused by exposure to phthalate chemicals in China reached approximately 57.2 billion yuan, with the burden of male infertility being the most severe. Further calculations indicate that the total medical cost for all known EDCs exposure in China is about 429.4 billion yuan, accounting for approximately 1.07% of the annual GDP.",{"type":12,"tag":21,"props":42,"children":43},{},[44],{"type":18,"value":30},{"type":12,"tag":21,"props":46,"children":47},{},[48],{"type":18,"value":49},"All these indicate that we must pay attention to these potential dangers and have a more comprehensive understanding and early screening of environmental endocrine disruptors. Nowadays, with the deepening research on environmental endocrine disruptors, more and more studies are beginning to focus on the relationship between endocrine disruptors and their metabolites and other toxic effects. This superposition of multiple effects provides clues to revealing the molecular mechanisms underlying many diseases.",{"type":12,"tag":13,"props":51,"children":53},{"id":52},"problem",[54],{"type":18,"value":55},"Problem",{"type":12,"tag":21,"props":57,"children":58},{},[59],{"type":18,"value":60},"In fact, the role of environmental endocrine disruptors in organisms is complex, and the inference of their adverse effects needs to emphasize the adverse outcome pathway (AOP pathway, which describes the logical derivation of causally related events at different levels of biological tissues, leading to adverse effects on human or wildlife health). This requires us to describe the molecular mechanisms behind the impact comprehensively.",{"type":12,"tag":21,"props":62,"children":63},{},[64],{"type":18,"value":65},"Currently, the methods for detecting endocrine disruptors using biological effects mainly include competitive ligand binding assays, fused cell aggregation experiments, and animal cell proliferation analysis experiments. These methods can only evaluate the endocrine disruption effect through the binding effect of hormone receptors when analyzing endocrine disruptors, and cannot judge the involvement of other effects behind the biological damage caused by endocrine disruptors, therefore, they have limitations.",{"type":12,"tag":21,"props":67,"children":68},{},[69],{"type":18,"value":70},"More and more literature has proven the important role of oxidative damage effects in the damage caused by environmental endocrine disruptors to organisms. For example, exposure to various parabens (PBs) and their metabolites can increase the level of oxidative stress, and the superposition of oxidative damage and endocrine disruption increases the risk of impaired reproductive capacity in organisms. The collaborative analysis of environmental endocrine disruption effects and oxidative damage effects can help us predict the pathways of action of environmental endocrine disruptors on organism damage, thereby assisting us in having a clearer understanding of their damage consequences and providing a reference for regulatory authorities to implement targeted regulatory policies.",{"type":12,"tag":21,"props":72,"children":73},{},[74],{"type":18,"value":75},"Our project is based on this concept and aims to construct microbial sensors through synthetic biology methods to provide multiple detection lines for the assessment of endocrine disruption effects and oxidative damage effects. Not only can we achieve the separate detection of the two effects, but we can also realize the collaborative analysis of the two effects through logic gate detection lines, achieving a rapid, convenient, and comprehensive assessment of environmental endocrine disruptors. Conduct more in-depth research and discussion on the impact mechanism of environmental endocrine disruptors.",{"type":12,"tag":13,"props":77,"children":79},{"id":78},"inspiration",[80],{"type":18,"value":81},"Inspiration",{"type":12,"tag":83,"props":84,"children":86},"h3",{"id":85},"biology",[87],{"type":18,"value":85},{"type":12,"tag":21,"props":89,"children":90},{},[91],{"type":18,"value":92},"Initially, we planned to develop a biosensor for detecting environmental endocrine disruptors. To achieve this goal, we studied the characteristics and mechanisms of action of environmental endocrine disruptors and researched the methods for detecting them. Intein peptides are protein analogs found in self-splicing RNA introns, and their insertion can lead to the inactivation of the host protein. Typically, they excise themselves from the host cell after translation, restoring the activity of the host protein. By replacing the homing endonuclease domain in the intein peptide with a hormone receptor binding domain, we can construct a hormone-sensitive intein peptide, which can be used to create recognition elements for endocrine disruption effects. We plan to construct an estrogen-sensitive intein peptide and insert it into T7 RNA Polymerase. T7 RNA Polymerase can specifically activate the T7 promoter, thus regulating the downstream circuit to express the reporter gene upon detection of estrogen mimics through the T7 promoter.",{"type":12,"tag":21,"props":94,"children":95},{},[96],{"type":18,"value":97},"Subsequently, through more in-depth researches, it was found that the effects of environmental endocrine disruptors in organisms are complex. An increasing number of studies have demonstrated the significant role of oxidative stress in the damage caused by environmental endocrine disruptors. For example, exposure to various paraben compounds (PBs) and their metabolites can increase oxidative stress levels. The combined oxidative damage and endocrine disruption can heighten the risk of impaired reproductive capability in organisms. A synergistic analysis of the effects of environmental endocrine disruption and oxidative damage can help us predict the pathways through which these disruptors cause harm to organisms, thereby assisting us in gaining a clearer understanding of their damaging consequences. Therefore, we plan to incorporate DNA damage detection, designing an and gate detection circuit capable of simultaneously detecting endocrine disruption effects and DNA oxidative damage effects.",{"type":12,"tag":21,"props":99,"children":100},{},[101,103,109],{"type":18,"value":102},"DNA damage detection is based on the SOS response mechanism in ",{"type":12,"tag":104,"props":105,"children":106},"em",{},[107],{"type":18,"value":108},"Escherichia coli",{"type":18,"value":110},", which is an inducible DNA repair system activated when bacterial DNA is damaged. When bacteria detect DNA damage, the expression levels of genes associated with the SOS response mechanism significantly increase, leading to the expression of proteases that relieve the repression of target proteins, initiating the repair process. We chose the recA promoter to detect DNA damage, when bacteria sense DNA damage, the expression level of this promoter rises significantly, thereby activating downstream reporter genes.",{"type":12,"tag":21,"props":112,"children":113},{},[114],{"type":18,"value":115},"To construct the logic gate, we introduced the hrp system, which consists of the hrpR and hrpS genes along with the HrpL promoter regulated by the sigma54 factor. The hrpL promoter is activated only when both hrpR and hrpS genes are expressed simultaneously.",{"type":12,"tag":21,"props":117,"children":118},{},[119],{"type":18,"value":120},"We plan to insert the hrpR gene downstream of the P recA promoter, which will be activated in response to DNA damage effects; the hrpS gene will be inserted downstream of the P T7 promoter, which will be activated after the peptide-specific binding domain binds to estrogen analogs. The reporter gene egfp will be inserted downstream of the P hrpL promoter, resulting in a two-input AND gate circuit.",{"type":12,"tag":21,"props":122,"children":123},{},[124],{"type":18,"value":125},"However, the entire circuit is too large, posing significant challenges to the stability of the plasmid and the burden on the engineered bacteria. Therefore, we plan to divide the entire circuit into two parts, each constructed in different engineered bacteria, inserting new components to enable signal transmission between the two engineered bacterias.",{"type":12,"tag":21,"props":127,"children":128},{},[129],{"type":18,"value":130},"Throughout the system, we have always planned to use the egfp gene as the reporter gene. However, this gene requires the presence of oxygen molecules as a cofactor for its luminescence, while the cultivation chamber of our designed hardware is in a low-oxygen environment. As a result, we tested a new reporter gene, BsFbFp, which can express significantly enhanced in vivo fluorescence in both aerobic and anaerobic environments, ensuring that the engineered bacteria can effectively express fluorescent signals in low-oxygen conditions.",{"type":12,"tag":21,"props":132,"children":133},{},[134],{"type":18,"value":135},"Thus, the construction of the biological part of our circuit has been completed.",{"type":12,"tag":83,"props":137,"children":139},{"id":138},"hardware",[140],{"type":18,"value":141},"Hardware",{"type":12,"tag":21,"props":143,"children":144},{},[145],{"type":18,"value":146},"Point of Care Testing (POCT) is a form of rapid on-site testing/near-patient testing method which has been particularly impactful and is playing an increasingly vital role in the fields of medical diagnostics and environmental health monitoring due to its speed, convenience, and ease of operation. Traditional water contaminant detection technologies are often constrained by the need for costly equipment and a professional laboratory environment. In contrast, novel biosensor detection devices based on compact POCT equipment, when connected to an interactive app for operational control and visual data analysis, can effectively facilitate the rapid and efficient screening of emerging waterborne pollutants. This approach holds broad prospects for application in the field of environmental monitoring and assessment.",{"type":12,"tag":13,"props":148,"children":150},{"id":149},"solution",[151],{"type":18,"value":152},"Solution",{"type":12,"tag":83,"props":154,"children":156},{"id":155},"biology-1",[157],{"type":18,"value":85},{"type":12,"tag":21,"props":159,"children":160},{},[161],{"type":18,"value":162},"To achieve signal communication between the two engineered bacteria, we studied the bacterial quorum sensing mechanism. This mechanism allows bacteria to exchange information and coordinate population behaviors when their numbers reach a certain density, regulating gene expression. When activated, bacteria secrete signal molecules that can freely diffuse across the cell membrane. Once the concentration of these signal molecules in the environment reaches a certain threshold, it induces the expression of specific genes in the bacteria. We introduced the LuxI/R system, where AHL (N-acyl homoserine lactone) is produced catalytically by the expression of the LuxI gene as a signaling molecule. 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Gut Microbes. 2022 Jan-Dec, 14(1):2039048. doi: 10.1080/19490976.2022.2039048.",{"type":12,"tag":227,"props":290,"children":291},{},[292],{"type":18,"value":293},"Drepper T, Eggert T, Circolone F, et al. Reporter proteins for in vivo fluorescence without oxygen. Nature Biotechnology, 2007, 25(4):443-445.DOI:10.1038/nbt1293.",{"type":12,"tag":227,"props":295,"children":296},{},[297,299,304],{"type":18,"value":298},"Mukherjee A, Schroeder C M, Sikes H D, et al. Flavin-based fluorescent proteins: emerging paradigms in biological imaging. ",{"type":12,"tag":104,"props":300,"children":301},{},[302],{"type":18,"value":303},"This review comes from a themed issue on Analytical biotechnology",{"type":18,"value":305},". 2015.",{"title":5,"searchDepth":307,"depth":308,"links":309},1,3,[310,312,313,317,321],{"id":15,"depth":311,"text":19},2,{"id":52,"depth":311,"text":55},{"id":78,"depth":311,"text":81,"children":314},[315,316],{"id":85,"depth":308,"text":85},{"id":138,"depth":308,"text":141},{"id":149,"depth":311,"text":152,"children":318},[319,320],{"id":155,"depth":308,"text":85},{"id":194,"depth":308,"text":141},{"id":211,"depth":311,"text":217},"markdown","content:project-description.md","content","project-description.md","project-description","md",1727871092285]
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.el-button--info:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button{--el-button-font-weight:var(--el-font-weight-primary);--el-button-border-color:var(--el-border-color);--el-button-bg-color:var(--el-fill-color-blank);--el-button-text-color:var(--el-text-color-regular);--el-button-disabled-text-color:var(--el-disabled-text-color);--el-button-disabled-bg-color:var(--el-fill-color-blank);--el-button-disabled-border-color:var(--el-border-color-light);--el-button-divide-border-color:hsla(0,0%,100%,.5);--el-button-hover-text-color:var(--el-color-primary);--el-button-hover-bg-color:var(--el-color-primary-light-9);--el-button-hover-border-color:var(--el-color-primary-light-7);--el-button-active-text-color:var(--el-button-hover-text-color);--el-button-active-border-color:var(--el-color-primary);--el-button-active-bg-color:var(--el-button-hover-bg-color);--el-button-outline-color:var(--el-color-primary-light-5);--el-button-hover-link-text-color:var(--el-color-info);--el-button-active-color:var(--el-text-color-primary);align-items:center;-webkit-appearance:none;background-color:var(--el-button-bg-color);border:var(--el-border);border-color:var(--el-button-border-color);box-sizing:border-box;color:var(--el-button-text-color);cursor:pointer;display:inline-flex;font-weight:var(--el-button-font-weight);height:32px;justify-content:center;line-height:1;outline:none;text-align:center;transition:.1s;-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-button:hover{background-color:var(--el-button-hover-bg-color);border-color:var(--el-button-hover-border-color);color:var(--el-button-hover-text-color);outline:none}.el-button:active{background-color:var(--el-button-active-bg-color);border-color:var(--el-button-active-border-color);color:var(--el-button-active-text-color);outline:none}.el-button:focus-visible{outline:2px 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1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper 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.el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags 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.el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large 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11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) 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cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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79.1666666667%;max-width:79.1666666667%}.el-col-xs-19.is-guttered{display:block}.el-col-xs-offset-19{margin-left:79.1666666667%}.el-col-xs-pull-19{position:relative;right:79.1666666667%}.el-col-xs-push-19{left:79.1666666667%;position:relative}.el-col-xs-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xs-20.is-guttered{display:block}.el-col-xs-offset-20{margin-left:83.3333333333%}.el-col-xs-pull-20{position:relative;right:83.3333333333%}.el-col-xs-push-20{left:83.3333333333%;position:relative}.el-col-xs-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xs-21.is-guttered{display:block}.el-col-xs-offset-21{margin-left:87.5%}.el-col-xs-pull-21{position:relative;right:87.5%}.el-col-xs-push-21{left:87.5%;position:relative}.el-col-xs-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 0%;max-width:0}.el-col-sm-0.is-guttered{display:none}.el-col-sm-offset-0{margin-left:0}.el-col-sm-pull-0{position:relative;right:0}.el-col-sm-push-0{left:0;position:relative}.el-col-sm-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-sm-1,.el-col-sm-1.is-guttered{display:block}.el-col-sm-offset-1{margin-left:4.1666666667%}.el-col-sm-pull-1{position:relative;right:4.1666666667%}.el-col-sm-push-1{left:4.1666666667%;position:relative}.el-col-sm-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-sm-2,.el-col-sm-2.is-guttered{display:block}.el-col-sm-offset-2{margin-left:8.3333333333%}.el-col-sm-pull-2{position:relative;right:8.3333333333%}.el-col-sm-push-2{left:8.3333333333%;position:relative}.el-col-sm-3{flex:0 0 12.5%;max-width:12.5%}.el-col-sm-3,.el-col-sm-3.is-guttered{display:block}.el-col-sm-offset-3{margin-left:12.5%}.el-col-sm-pull-3{position:relative;right:12.5%}.el-col-sm-push-3{left:12.5%;position:relative}.el-col-sm-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-sm-4,.el-col-sm-4.is-guttered{display:block}.el-col-sm-offset-4{margin-left:16.6666666667%}.el-col-sm-pull-4{position:relative;right:16.6666666667%}.el-col-sm-push-4{left:16.6666666667%;position:relative}.el-col-sm-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-sm-5,.el-col-sm-5.is-guttered{display:block}.el-col-sm-offset-5{margin-left:20.8333333333%}.el-col-sm-pull-5{position:relative;right:20.8333333333%}.el-col-sm-push-5{left:20.8333333333%;position:relative}.el-col-sm-6{flex:0 0 25%;max-width:25%}.el-col-sm-6,.el-col-sm-6.is-guttered{display:block}.el-col-sm-offset-6{margin-left:25%}.el-col-sm-pull-6{position:relative;right:25%}.el-col-sm-push-6{left:25%;position:relative}.el-col-sm-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 0%;max-width:0}.el-col-xl-0.is-guttered{display:none}.el-col-xl-offset-0{margin-left:0}.el-col-xl-pull-0{position:relative;right:0}.el-col-xl-push-0{left:0;position:relative}.el-col-xl-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-xl-1,.el-col-xl-1.is-guttered{display:block}.el-col-xl-offset-1{margin-left:4.1666666667%}.el-col-xl-pull-1{position:relative;right:4.1666666667%}.el-col-xl-push-1{left:4.1666666667%;position:relative}.el-col-xl-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-xl-2,.el-col-xl-2.is-guttered{display:block}.el-col-xl-offset-2{margin-left:8.3333333333%}.el-col-xl-pull-2{position:relative;right:8.3333333333%}.el-col-xl-push-2{left:8.3333333333%;position:relative}.el-col-xl-3{flex:0 0 12.5%;max-width:12.5%}.el-col-xl-3,.el-col-xl-3.is-guttered{display:block}.el-col-xl-offset-3{margin-left:12.5%}.el-col-xl-pull-3{position:relative;right:12.5%}.el-col-xl-push-3{left:12.5%;position:relative}.el-col-xl-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-xl-4,.el-col-xl-4.is-guttered{display:block}.el-col-xl-offset-4{margin-left:16.6666666667%}.el-col-xl-pull-4{position:relative;right:16.6666666667%}.el-col-xl-push-4{left:16.6666666667%;position:relative}.el-col-xl-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-xl-5,.el-col-xl-5.is-guttered{display:block}.el-col-xl-offset-5{margin-left:20.8333333333%}.el-col-xl-pull-5{position:relative;right:20.8333333333%}.el-col-xl-push-5{left:20.8333333333%;position:relative}.el-col-xl-6{flex:0 0 25%;max-width:25%}.el-col-xl-6,.el-col-xl-6.is-guttered{display:block}.el-col-xl-offset-6{margin-left:25%}.el-col-xl-pull-6{position:relative;right:25%}.el-col-xl-push-6{left:25%;position:relative}.el-col-xl-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-xl-10.is-guttered{display:block}.el-col-xl-offset-10{margin-left:41.6666666667%}.el-col-xl-pull-10{position:relative;right:41.6666666667%}.el-col-xl-push-10{left:41.6666666667%;position:relative}.el-col-xl-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-xl-11.is-guttered{display:block}.el-col-xl-offset-11{margin-left:45.8333333333%}.el-col-xl-pull-11{position:relative;right:45.8333333333%}.el-col-xl-push-11{left:45.8333333333%;position:relative}.el-col-xl-12{display:block;flex:0 0 50%;max-width:50%}.el-col-xl-12.is-guttered{display:block}.el-col-xl-offset-12{margin-left:50%}.el-col-xl-pull-12{position:relative;right:50%}.el-col-xl-push-12{left:50%;position:relative}.el-col-xl-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px;box-sizing:border-box;height:12px;position:relative;width:280px}.el-color-alpha-slider__bar{background:linear-gradient(to right,hsla(0,0%,100%,0) 0,var(--el-bg-color) 100%);height:100%;position:relative}.el-color-alpha-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-alpha-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-picker.is-focused .el-color-picker__trigger{border-color:var(--el-color-primary)}.el-color-picker.is-disabled .el-color-picker__trigger{cursor:not-allowed}.el-color-picker--large{height:40px}.el-color-picker--large .el-color-picker__trigger{height:40px;width:40px}.el-color-picker--large .el-color-picker__mask{height:38px;width:38px}.el-color-picker--small{height:24px}.el-color-picker--small .el-color-picker__trigger{height:24px;width:24px}.el-color-picker--small .el-color-picker__mask{height:22px;width:22px}.el-color-picker--small .el-color-picker__empty,.el-color-picker--small .el-color-picker__icon{transform:scale(.8)}.el-color-picker__mask{background-color:hsla(0,0%,100%,.7);border-radius:4px;cursor:not-allowed;height:30px;left:1px;position:absolute;top:1px;width:30px;z-index:1}.el-color-picker__trigger{align-items:center;border:1px solid var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker 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5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid 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var(--el-transition-duration)}.dialog-fade-leave-active .el-overlay-dialog{animation:dialog-fade-out var(--el-transition-duration)}@keyframes dialog-fade-in{0%{opacity:0;transform:translate3d(0,-20px,0)}to{opacity:1;transform:translateZ(0)}}@keyframes dialog-fade-out{0%{opacity:1;transform:translateZ(0)}to{opacity:0;transform:translate3d(0,-20px,0)}}@keyframes modal-fade-in{0%{opacity:0}to{opacity:1}}@keyframes modal-fade-out{0%{opacity:1}to{opacity:0}}.el-divider{position:relative}.el-divider--horizontal{border-top:1px var(--el-border-color) var(--el-border-style);display:block;height:1px;margin:24px 0;width:100%}.el-divider--vertical{border-left:1px var(--el-border-color) var(--el-border-style);display:inline-block;height:1em;margin:0 8px;position:relative;vertical-align:middle;width:1px}.el-divider__text{background-color:var(--el-bg-color);color:var(--el-text-color-primary);font-size:14px;font-weight:500;padding:0 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.ttb{transform:translate(0)}.el-drawer__sr-focus:focus{outline:none!important}.el-drawer__header{align-items:center;color:#72767b;display:flex;margin-bottom:32px;padding:var(--el-drawer-padding-primary);padding-bottom:0}.el-drawer__header>:first-child{flex:1}.el-drawer__title{flex:1;font-size:16px;line-height:inherit;margin:0}.el-drawer__footer{padding:var(--el-drawer-padding-primary);padding-top:10px;text-align:right}.el-drawer__close-btn{background-color:transparent;border:none;color:inherit;cursor:pointer;display:inline-flex;font-size:var(--el-font-size-extra-large);outline:none}.el-drawer__close-btn:focus i,.el-drawer__close-btn:hover 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.el-popper__arrow:before{border:1px solid var(--el-border-color-light)}.el-dropdown__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-dropdown__popper .el-dropdown-menu{border:none}.el-dropdown__popper .el-dropdown__popper-selfdefine{outline:none}.el-dropdown__popper .el-scrollbar__bar{z-index:calc(var(--el-dropdown-menu-index) + 1)}.el-dropdown__popper .el-dropdown__list{box-sizing:border-box;list-style:none;margin:0;padding:0}.el-dropdown 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var(--el-transition-duration);width:var(--el-notification-width);z-index:9999}.el-notification.right{right:16px}.el-notification.left{left:16px}.el-notification__group{margin-left:var(--el-notification-group-margin-left);margin-right:var(--el-notification-group-margin-right)}.el-notification__title{color:var(--el-notification-title-color);font-size:var(--el-notification-title-font-size);font-weight:700;line-height:var(--el-notification-icon-size);margin:0}.el-notification__content{color:var(--el-notification-content-color);font-size:var(--el-notification-content-font-size);line-height:24px;margin:6px 0 0}.el-notification__content p{margin:0}.el-notification .el-notification__icon{font-size:var(--el-notification-icon-size);height:var(--el-notification-icon-size);width:var(--el-notification-icon-size)}.el-notification .el-notification__closeBtn{color:var(--el-notification-close-color);cursor:pointer;font-size:var(--el-notification-close-font-size);position:absolute;right:15px;top:18px}.el-notification .el-notification__closeBtn:hover{color:var(--el-notification-close-hover-color)}.el-notification .el-notification--success{--el-notification-icon-color:var(--el-color-success);color:var(--el-notification-icon-color)}.el-notification .el-notification--info{--el-notification-icon-color:var(--el-color-info);color:var(--el-notification-icon-color)}.el-notification .el-notification--warning{--el-notification-icon-color:var(--el-color-warning);color:var(--el-notification-icon-color)}.el-notification .el-notification--error{--el-notification-icon-color:var(--el-color-error);color:var(--el-notification-icon-color)}.el-notification-fade-enter-from.right{right:0;transform:translateX(100%)}.el-notification-fade-enter-from.left{left:0;transform:translateX(-100%)}.el-notification-fade-leave-to{opacity:0}.el-overlay{background-color:var(--el-overlay-color-lighter);bottom:0;height:100%;left:0;overflow:auto;position:fixed;right:0;top:0;z-index:2000}.el-overlay .el-overlay-root{height:0}.el-page-header.is-contentful .el-page-header__main{border-top:1px solid var(--el-border-color-light);margin-top:16px}.el-page-header__header{align-items:center;display:flex;justify-content:space-between;line-height:24px}.el-page-header__left{align-items:center;display:flex;margin-right:40px;position:relative}.el-page-header__back{align-items:center;cursor:pointer;display:flex}.el-page-header__left .el-divider--vertical{margin:0 16px}.el-page-header__icon{align-items:center;display:flex;font-size:16px;margin-right:10px}.el-page-header__icon .el-icon{font-size:inherit}.el-page-header__title{font-size:14px;font-weight:500}.el-page-header__content{color:var(--el-text-color-primary);font-size:18px}.el-page-header__breadcrumb{margin-bottom:16px}.el-pagination{--el-pagination-font-size:14px;--el-pagination-bg-color:var(--el-fill-color-blank);--el-pagination-text-color:var(--el-text-color-primary);--el-pagination-border-radius:2px;--el-pagination-button-color:var(--el-text-color-primary);--el-pagination-button-width:32px;--el-pagination-button-height:32px;--el-pagination-button-disabled-color:var(--el-text-color-placeholder);--el-pagination-button-disabled-bg-color:var(--el-fill-color-blank);--el-pagination-button-bg-color:var(--el-fill-color);--el-pagination-hover-color:var(--el-color-primary);--el-pagination-font-size-small:12px;--el-pagination-button-width-small:24px;--el-pagination-button-height-small:24px;--el-pagination-button-width-large:40px;--el-pagination-button-height-large:40px;--el-pagination-item-gap:16px;align-items:center;color:var(--el-pagination-text-color);display:flex;font-size:var(--el-pagination-font-size);font-weight:400;white-space:nowrap}.el-pagination .el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small 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.el-transfer-panel__header{align-items:center;background:var(--el-transfer-panel-header-bg-color);border:1px solid var(--el-transfer-border-color);border-top-left-radius:var(--el-transfer-border-radius);border-top-right-radius:var(--el-transfer-border-radius);box-sizing:border-box;color:var(--el-color-black);display:flex;height:var(--el-transfer-panel-header-height);margin:0;padding-left:15px}.el-transfer-panel .el-transfer-panel__header .el-checkbox{align-items:center;display:flex;position:relative;width:100%}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label{color:var(--el-text-color-primary);font-size:16px;font-weight:400}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label span{color:var(--el-text-color-secondary);font-size:12px;font-weight:400;position:absolute;right:15px;top:50%;transform:translate3d(0,-50%,0)}.el-transfer-panel .el-transfer-panel__footer{background:var(--el-bg-color-overlay);border:1px solid 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0;overflow:hidden;padding:0;width:var(--el-upload-list-picture-card-size)}.el-upload-list--picture-card .el-upload-list__item .el-icon--check,.el-upload-list--picture-card .el-upload-list__item .el-icon--circle-check{color:#fff}.el-upload-list--picture-card .el-upload-list__item .el-icon--close{display:none}.el-upload-list--picture-card .el-upload-list__item:hover .el-upload-list__item-status-label{display:block;opacity:0}.el-upload-list--picture-card .el-upload-list__item:hover .el-progress__text{display:block}.el-upload-list--picture-card .el-upload-list__item .el-upload-list__item-name{display:none}.el-upload-list--picture-card .el-upload-list__item-thumbnail{height:100%;-o-object-fit:contain;object-fit:contain;width:100%}.el-upload-list--picture-card .el-upload-list__item-status-label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-list--picture-card .el-upload-list__item-status-label 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img{display:block;height:100%;width:100%}.el-upload-cover__label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-cover__label i{color:#fff;font-size:12px;margin-top:11px;transform:rotate(-45deg)}.el-upload-cover__progress{display:inline-block;position:static;vertical-align:middle;width:243px}.el-upload-cover__progress+.el-upload__inner{opacity:0}.el-upload-cover__content{height:100%;left:0;position:absolute;top:0;width:100%}.el-upload-cover__interact{background-color:var(--el-overlay-color-light);bottom:0;height:100%;left:0;position:absolute;text-align:center;width:100%}.el-upload-cover__interact .btn{color:#fff;cursor:pointer;display:inline-block;font-size:14px;margin-top:60px;transition:var(--el-transition-md-fade);vertical-align:middle}.el-upload-cover__interact .btn i{margin-top:0}.el-upload-cover__interact .btn span{opacity:0;transition:opacity .15s linear}.el-upload-cover__interact 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var(--el-border-color-light)}.el-popper.is-light>.el-popper__arrow:before{right:0}.el-popper.is-pure{padding:0}.el-popper__arrow,.el-popper__arrow:before{height:10px;position:absolute;width:10px;z-index:-1}.el-popper__arrow:before{background:var(--el-text-color-primary);box-sizing:border-box;content:" 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srcset="https://static.igem.wiki/teams/5358/banner/project.webp 1x, https://static.igem.wiki/teams/5358/banner/project.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/project.webp"><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg">Description</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[300px] md:mr-10"><h1 class="mt-0">TOC</h1><ul><!--[--><li class="my-5"><a class="cursor-pointer">Background</a></li><li class="my-5"><a class="cursor-pointer">Problem</a></li><li class="my-5"><a class="cursor-pointer">Inspiration</a></li><li class="my-5"><a class="cursor-pointer">Solution</a></li><li class="my-5"><a class="cursor-pointer">Reference</a></li><!--]--></ul></aside><div class="content"><h2 id="background"><!--[-->Background<!--]--></h2><p><!--[-->Environmental endocrine disruptors (EDCs) are a class of chemical substances that can mimic or block the effects of hormones, affecting the normal development of organisms. These substances include DDT, dioxins, and phthalates, among others.<!--]--></p><p><!--[-->...<!--]--></p><p><!--[-->DDT is an insecticide that was once widely used, but due to its harmful effects on the reproductive and nervous systems of organisms, as well as its potential carcinogenicity, its use has been restricted in many countries since the 1970s and 1980s. However, as these substances have already entered the environment in large quantities, their negative effects persist even though their production has stopped for many years. They accumulate in the atmosphere, soil, water bodies, and organisms, posing a natural hazard.<!--]--></p><p><!--[-->In 2010, the direct social and economic loss caused by exposure to phthalate chemicals in China reached approximately 57.2 billion yuan, with the burden of male infertility being the most severe. Further calculations indicate that the total medical cost for all known EDCs exposure in China is about 429.4 billion yuan, accounting for approximately 1.07% of the annual GDP.<!--]--></p><p><!--[-->...<!--]--></p><p><!--[-->All these indicate that we must pay attention to these potential dangers and have a more comprehensive understanding and early screening of environmental endocrine disruptors. Nowadays, with the deepening research on environmental endocrine disruptors, more and more studies are beginning to focus on the relationship between endocrine disruptors and their metabolites and other toxic effects. This superposition of multiple effects provides clues to revealing the molecular mechanisms underlying many diseases.<!--]--></p><h2 id="problem"><!--[-->Problem<!--]--></h2><p><!--[-->In fact, the role of environmental endocrine disruptors in organisms is complex, and the inference of their adverse effects needs to emphasize the adverse outcome pathway (AOP pathway, which describes the logical derivation of causally related events at different levels of biological tissues, leading to adverse effects on human or wildlife health). This requires us to describe the molecular mechanisms behind the impact comprehensively.<!--]--></p><p><!--[-->Currently, the methods for detecting endocrine disruptors using biological effects mainly include competitive ligand binding assays, fused cell aggregation experiments, and animal cell proliferation analysis experiments. These methods can only evaluate the endocrine disruption effect through the binding effect of hormone receptors when analyzing endocrine disruptors, and cannot judge the involvement of other effects behind the biological damage caused by endocrine disruptors, therefore, they have limitations.<!--]--></p><p><!--[-->More and more literature has proven the important role of oxidative damage effects in the damage caused by environmental endocrine disruptors to organisms. For example, exposure to various parabens (PBs) and their metabolites can increase the level of oxidative stress, and the superposition of oxidative damage and endocrine disruption increases the risk of impaired reproductive capacity in organisms. The collaborative analysis of environmental endocrine disruption effects and oxidative damage effects can help us predict the pathways of action of environmental endocrine disruptors on organism damage, thereby assisting us in having a clearer understanding of their damage consequences and providing a reference for regulatory authorities to implement targeted regulatory policies.<!--]--></p><p><!--[-->Our project is based on this concept and aims to construct microbial sensors through synthetic biology methods to provide multiple detection lines for the assessment of endocrine disruption effects and oxidative damage effects. Not only can we achieve the separate detection of the two effects, but we can also realize the collaborative analysis of the two effects through logic gate detection lines, achieving a rapid, convenient, and comprehensive assessment of environmental endocrine disruptors. Conduct more in-depth research and discussion on the impact mechanism of environmental endocrine disruptors.<!--]--></p><h2 id="inspiration"><!--[-->Inspiration<!--]--></h2><h3 id="biology"><!--[-->biology<!--]--></h3><p><!--[-->Initially, we planned to develop a biosensor for detecting environmental endocrine disruptors. To achieve this goal, we studied the characteristics and mechanisms of action of environmental endocrine disruptors and researched the methods for detecting them. Intein peptides are protein analogs found in self-splicing RNA introns, and their insertion can lead to the inactivation of the host protein. Typically, they excise themselves from the host cell after translation, restoring the activity of the host protein. By replacing the homing endonuclease domain in the intein peptide with a hormone receptor binding domain, we can construct a hormone-sensitive intein peptide, which can be used to create recognition elements for endocrine disruption effects. We plan to construct an estrogen-sensitive intein peptide and insert it into T7 RNA Polymerase. T7 RNA Polymerase can specifically activate the T7 promoter, thus regulating the downstream circuit to express the reporter gene upon detection of estrogen mimics through the T7 promoter.<!--]--></p><p><!--[-->Subsequently, through more in-depth researches, it was found that the effects of environmental endocrine disruptors in organisms are complex. An increasing number of studies have demonstrated the significant role of oxidative stress in the damage caused by environmental endocrine disruptors. For example, exposure to various paraben compounds (PBs) and their metabolites can increase oxidative stress levels. The combined oxidative damage and endocrine disruption can heighten the risk of impaired reproductive capability in organisms. A synergistic analysis of the effects of environmental endocrine disruption and oxidative damage can help us predict the pathways through which these disruptors cause harm to organisms, thereby assisting us in gaining a clearer understanding of their damaging consequences. Therefore, we plan to incorporate DNA damage detection, designing an and gate detection circuit capable of simultaneously detecting endocrine disruption effects and DNA oxidative damage effects.<!--]--></p><p><!--[-->DNA damage detection is based on the SOS response mechanism in <em><!--[-->Escherichia coli<!--]--></em>, which is an inducible DNA repair system activated when bacterial DNA is damaged. When bacteria detect DNA damage, the expression levels of genes associated with the SOS response mechanism significantly increase, leading to the expression of proteases that relieve the repression of target proteins, initiating the repair process. We chose the recA promoter to detect DNA damage, when bacteria sense DNA damage, the expression level of this promoter rises significantly, thereby activating downstream reporter genes.<!--]--></p><p><!--[-->To construct the logic gate, we introduced the hrp system, which consists of the hrpR and hrpS genes along with the HrpL promoter regulated by the sigma54 factor. The hrpL promoter is activated only when both hrpR and hrpS genes are expressed simultaneously.<!--]--></p><p><!--[-->We plan to insert the hrpR gene downstream of the P recA promoter, which will be activated in response to DNA damage effects; the hrpS gene will be inserted downstream of the P T7 promoter, which will be activated after the peptide-specific binding domain binds to estrogen analogs. The reporter gene egfp will be inserted downstream of the P hrpL promoter, resulting in a two-input AND gate circuit.<!--]--></p><p><!--[-->However, the entire circuit is too large, posing significant challenges to the stability of the plasmid and the burden on the engineered bacteria. Therefore, we plan to divide the entire circuit into two parts, each constructed in different engineered bacteria, inserting new components to enable signal transmission between the two engineered bacterias.<!--]--></p><p><!--[-->Throughout the system, we have always planned to use the egfp gene as the reporter gene. However, this gene requires the presence of oxygen molecules as a cofactor for its luminescence, while the cultivation chamber of our designed hardware is in a low-oxygen environment. As a result, we tested a new reporter gene, BsFbFp, which can express significantly enhanced in vivo fluorescence in both aerobic and anaerobic environments, ensuring that the engineered bacteria can effectively express fluorescent signals in low-oxygen conditions.<!--]--></p><p><!--[-->Thus, the construction of the biological part of our circuit has been completed.<!--]--></p><h3 id="hardware"><!--[-->Hardware<!--]--></h3><p><!--[-->Point of Care Testing (POCT) is a form of rapid on-site testing/near-patient testing method which has been particularly impactful and is playing an increasingly vital role in the fields of medical diagnostics and environmental health monitoring due to its speed, convenience, and ease of operation. Traditional water contaminant detection technologies are often constrained by the need for costly equipment and a professional laboratory environment. In contrast, novel biosensor detection devices based on compact POCT equipment, when connected to an interactive app for operational control and visual data analysis, can effectively facilitate the rapid and efficient screening of emerging waterborne pollutants. This approach holds broad prospects for application in the field of environmental monitoring and assessment.<!--]--></p><h2 id="solution"><!--[-->Solution<!--]--></h2><h3 id="biology-1"><!--[-->biology<!--]--></h3><p><!--[-->To achieve signal communication between the two engineered bacteria, we studied the bacterial quorum sensing mechanism. This mechanism allows bacteria to exchange information and coordinate population behaviors when their numbers reach a certain density, regulating gene expression. When activated, bacteria secrete signal molecules that can freely diffuse across the cell membrane. Once the concentration of these signal molecules in the environment reaches a certain threshold, it induces the expression of specific genes in the bacteria. We introduced the LuxI/R system, where AHL (N-acyl homoserine lactone) is produced catalytically by the expression of the LuxI gene as a signaling molecule. This AHL freely diffuses into the second engineered bacteria and binds with the LuxR protein released by the activation of the LuxR gene, activating the P LuxR promoter to initiate downstream gene expression.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/description/biocircuit-p1.png 1x, https://static.igem.wiki/teams/5358/description/biocircuit-p1.png 2x" src="https://static.igem.wiki/teams/5358/description/biocircuit-p1.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/description/biocircuit-p2.png 1x, https://static.igem.wiki/teams/5358/description/biocircuit-p2.png 2x" src="https://static.igem.wiki/teams/5358/description/biocircuit-p2.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/description/biocircuit-p3.png 1x, https://static.igem.wiki/teams/5358/description/biocircuit-p3.png 2x" src="https://static.igem.wiki/teams/5358/description/biocircuit-p3.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/description/biocircuit-p4.png 1x, https://static.igem.wiki/teams/5358/description/biocircuit-p4.png 2x" src="https://static.igem.wiki/teams/5358/description/biocircuit-p4.png"><!--]--></p><h3 id="hardware-1"><!--[-->Hardware<!--]--></h3><p><!--[-->In response to the demands for standardized cultivation of engineered bacteria and high-sensitivity detection, we have designed a highly integrated and automated system that encompasses the entire process of bacterial strain revival, cultivation, detection, and analysis. The system utilizes an ESP32 chip as the main controller, interfaces with a temperature control system and a fluorescence detection system, employs a linear slide rail structure for high-throughput detection, and leverages a mobile app for automated device control and visualization of detection results. This system overcomes the limitations of time and space associated with traditional detection equipment, providing a versatile, portable, and highly sensitive solution for the detection of EDCs in aquatic environments across various scenarios.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/description/and-gate.png 1x, https://static.igem.wiki/teams/5358/description/and-gate.png 2x" src="https://static.igem.wiki/teams/5358/description/and-gate.png"><!--]--></p><h2 id="reference"><!--[--><em><!--[-->Reference<!--]--></em><!--]--></h2><div class="reference"><!--[--><ol><!--[--><li><!--[-->Cao Y, Li L, Shen KH, Liu JG. Disease burden attributable to endocrine-disrupting chemicals exposure in China: A case study of phthalates. Science of the Total Environment, 2019, Vol. 662(1): 615-621<!--]--></li><li><!--[-->Nematollah Asadi, Mahmoud Bahmani, Arash Kheradmand, Mahmoud Rafieian-Kopaei. The Impact of Oxidative Stress on Testicular Function and the Role of Antioxidants in Improving it: A Review. Journal of Clinical and Diagnostic Research, 2017, Vol. 11(5): IE1-IE5<!--]--></li><li><!--[-->Ma Ya, Liu Haohao, Wu Jinxia, Yuan Le, Wang Yueqin, Du Xingde, Wang Rui, Marwa Phelisters Wegesa, Petlulu Pavankumar, Chen Xinghai, Zhang Huizhen. The adverse health effects of bisphenol A and related toxicity mechanisms. Environmental Research, 2019, Vol. 176: 108<!--]--></li><li><!--[-->Liang R , Zhou J , Liu J. Construction of a Bacterial Assay for Estrogen Detection Based on an Estrogen-Sensitive Intein. Applied &amp; Environmental Microbiology, 2011, 77(7):2488.DOI:10.1128/AEM.02336-10.<!--]--></li><li><!--[-->Ma Ya, Liu Haohao, Wu Jinxia, Yuan Le, Wang Yueqin, Du Xingde, Wang Rui, Marwa, Phelisters Wegesa, Petlulu, Pavankumar, Chen Xinghai, Zhang Huizhen.The adverse health effects of bisphenol A and related toxicity mechanisms.Environmental Research, 2019, Vol.176: 108575<!--]--></li><li><!--[-->LIANG Jing-jia, GU Ai-hua. Multigenerational and cross-generational effect of environmental endocrine disruptors on reproductive system in male animals. <em><!--[-->Chinese Journal of Public Health<!--]--></em>, 2021, 37(2): 375-380.<!--]--></li><li><!--[-->Nematollah Asadi, Mahmoud Bahmani, Arash Kheradmand, Mahmoud Rafieian-Kopaei.The Impact of Oxidative Stress on Testicular Function and the Role of Antioxidants in Improving it: A Review.Journal of Clinical and Diagnostic Research, 2017, Vol.11(5): IE1-IE5<!--]--></li><li><!--[-->Mondal Shirsha, Ghosh Songita, Bhattacharya Samir, Mukherjee Sutapa. Chronic dietary administration of lower levels of diethyl phthalate induces murine testicular germ cell inflammation and sperm pathologies: Involvement of oxidative stress. Chemosphere, 2019, Vol.229: 443-451<!--]--></li><li><!--[-->Maslowska K H, Karolina Makiela‐Dzbenska, Fijalkowska I J. The SOS system: A complex and tightly regulated response to DNA damage. John Wiley &amp; Sons, Ltd, 2019(4). DOI:10.1002/em.22267.<!--]--></li><li><!--[-->Baojun W, Mauricio B, Martin B. Engineering modular and tunable genetic amplifiers for scaling transcriptional signals in cascaded gene networks. Nucleic Acids Research, 2014(14): 9484-9492. DOI:10.1093/nar/gku593.<!--]--></li><li><!--[-->Xiao Y, Zou H, Li J, Song T, Lv W, Wang W, Wang Z, Tao S. Impact of quorum sensing signaling molecules in gram-negative bacteria on host cells: current understanding and future perspectives. Gut Microbes. 2022 Jan-Dec, 14(1):2039048. doi: 10.1080/19490976.2022.2039048.<!--]--></li><li><!--[-->Drepper T, Eggert T, Circolone F, et al. Reporter proteins for in vivo fluorescence without oxygen. Nature Biotechnology, 2007, 25(4):443-445.DOI:10.1038/nbt1293.<!--]--></li><li><!--[-->Mukherjee A, Schroeder C M, Sikes H D, et al. Flavin-based fluorescent proteins: emerging paradigms in biological imaging. <em><!--[-->This review comes from a themed issue on Analytical biotechnology<!--]--></em>. 2015.<!--]--></li><!--]--></ol><!--]--></div></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a aria-current="page" href="/bit/description" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/description/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-Pcus8P4khp":11},null,"/description",1727868995247]</script>
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srcset="https://static.igem.wiki/teams/5358/banner/project.webp 1x, https://static.igem.wiki/teams/5358/banner/project.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/project.webp"><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg">Description</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[300px] md:mr-10"><h1 class="mt-0">TOC</h1><ul><!--[--><li class="my-5"><a class="cursor-pointer">Background</a></li><li class="my-5"><a class="cursor-pointer">Problem</a></li><li class="my-5"><a class="cursor-pointer">Inspiration</a></li><li class="my-5"><a class="cursor-pointer">Solution</a></li><li class="my-5"><a class="cursor-pointer">Reference</a></li><!--]--></ul></aside><div class="content"><h2 id="background"><!--[-->Background<!--]--></h2><p><!--[-->Environmental endocrine disruptors (EDCs) are a class of chemical substances that can mimic or block the effects of hormones, affecting the normal development of organisms. These substances include DDT, dioxins, and phthalates, among others.<!--]--></p><p><!--[-->...<!--]--></p><p><!--[-->DDT is an insecticide that was once widely used, but due to its harmful effects on the reproductive and nervous systems of organisms, as well as its potential carcinogenicity, its use has been restricted in many countries since the 1970s and 1980s. However, as these substances have already entered the environment in large quantities, their negative effects persist even though their production has stopped for many years. They accumulate in the atmosphere, soil, water bodies, and organisms, posing a natural hazard.<!--]--></p><p><!--[-->In 2010, the direct social and economic loss caused by exposure to phthalate chemicals in China reached approximately 57.2 billion yuan, with the burden of male infertility being the most severe. Further calculations indicate that the total medical cost for all known EDCs exposure in China is about 429.4 billion yuan, accounting for approximately 1.07% of the annual GDP.<!--]--></p><p><!--[-->...<!--]--></p><p><!--[-->All these indicate that we must pay attention to these potential dangers and have a more comprehensive understanding and early screening of environmental endocrine disruptors. Nowadays, with the deepening research on environmental endocrine disruptors, more and more studies are beginning to focus on the relationship between endocrine disruptors and their metabolites and other toxic effects. This superposition of multiple effects provides clues to revealing the molecular mechanisms underlying many diseases.<!--]--></p><h2 id="problem"><!--[-->Problem<!--]--></h2><p><!--[-->In fact, the role of environmental endocrine disruptors in organisms is complex, and the inference of their adverse effects needs to emphasize the adverse outcome pathway (AOP pathway, which describes the logical derivation of causally related events at different levels of biological tissues, leading to adverse effects on human or wildlife health). This requires us to describe the molecular mechanisms behind the impact comprehensively.<!--]--></p><p><!--[-->Currently, the methods for detecting endocrine disruptors using biological effects mainly include competitive ligand binding assays, fused cell aggregation experiments, and animal cell proliferation analysis experiments. These methods can only evaluate the endocrine disruption effect through the binding effect of hormone receptors when analyzing endocrine disruptors, and cannot judge the involvement of other effects behind the biological damage caused by endocrine disruptors, therefore, they have limitations.<!--]--></p><p><!--[-->More and more literature has proven the important role of oxidative damage effects in the damage caused by environmental endocrine disruptors to organisms. For example, exposure to various parabens (PBs) and their metabolites can increase the level of oxidative stress, and the superposition of oxidative damage and endocrine disruption increases the risk of impaired reproductive capacity in organisms. The collaborative analysis of environmental endocrine disruption effects and oxidative damage effects can help us predict the pathways of action of environmental endocrine disruptors on organism damage, thereby assisting us in having a clearer understanding of their damage consequences and providing a reference for regulatory authorities to implement targeted regulatory policies.<!--]--></p><p><!--[-->Our project is based on this concept and aims to construct microbial sensors through synthetic biology methods to provide multiple detection lines for the assessment of endocrine disruption effects and oxidative damage effects. Not only can we achieve the separate detection of the two effects, but we can also realize the collaborative analysis of the two effects through logic gate detection lines, achieving a rapid, convenient, and comprehensive assessment of environmental endocrine disruptors. Conduct more in-depth research and discussion on the impact mechanism of environmental endocrine disruptors.<!--]--></p><h2 id="inspiration"><!--[-->Inspiration<!--]--></h2><h3 id="biology"><!--[-->biology<!--]--></h3><p><!--[-->Initially, we planned to develop a biosensor for detecting environmental endocrine disruptors. To achieve this goal, we studied the characteristics and mechanisms of action of environmental endocrine disruptors and researched the methods for detecting them. Intein peptides are protein analogs found in self-splicing RNA introns, and their insertion can lead to the inactivation of the host protein. Typically, they excise themselves from the host cell after translation, restoring the activity of the host protein. By replacing the homing endonuclease domain in the intein peptide with a hormone receptor binding domain, we can construct a hormone-sensitive intein peptide, which can be used to create recognition elements for endocrine disruption effects. We plan to construct an estrogen-sensitive intein peptide and insert it into T7 RNA Polymerase. T7 RNA Polymerase can specifically activate the T7 promoter, thus regulating the downstream circuit to express the reporter gene upon detection of estrogen mimics through the T7 promoter.<!--]--></p><p><!--[-->Subsequently, through more in-depth researches, it was found that the effects of environmental endocrine disruptors in organisms are complex. An increasing number of studies have demonstrated the significant role of oxidative stress in the damage caused by environmental endocrine disruptors. For example, exposure to various paraben compounds (PBs) and their metabolites can increase oxidative stress levels. The combined oxidative damage and endocrine disruption can heighten the risk of impaired reproductive capability in organisms. A synergistic analysis of the effects of environmental endocrine disruption and oxidative damage can help us predict the pathways through which these disruptors cause harm to organisms, thereby assisting us in gaining a clearer understanding of their damaging consequences. Therefore, we plan to incorporate DNA damage detection, designing an and gate detection circuit capable of simultaneously detecting endocrine disruption effects and DNA oxidative damage effects.<!--]--></p><p><!--[-->DNA damage detection is based on the SOS response mechanism in <em><!--[-->Escherichia coli<!--]--></em>, which is an inducible DNA repair system activated when bacterial DNA is damaged. When bacteria detect DNA damage, the expression levels of genes associated with the SOS response mechanism significantly increase, leading to the expression of proteases that relieve the repression of target proteins, initiating the repair process. We chose the recA promoter to detect DNA damage, when bacteria sense DNA damage, the expression level of this promoter rises significantly, thereby activating downstream reporter genes.<!--]--></p><p><!--[-->To construct the logic gate, we introduced the hrp system, which consists of the hrpR and hrpS genes along with the HrpL promoter regulated by the sigma54 factor. The hrpL promoter is activated only when both hrpR and hrpS genes are expressed simultaneously.<!--]--></p><p><!--[-->We plan to insert the hrpR gene downstream of the P recA promoter, which will be activated in response to DNA damage effects; the hrpS gene will be inserted downstream of the P T7 promoter, which will be activated after the peptide-specific binding domain binds to estrogen analogs. The reporter gene egfp will be inserted downstream of the P hrpL promoter, resulting in a two-input AND gate circuit.<!--]--></p><p><!--[-->However, the entire circuit is too large, posing significant challenges to the stability of the plasmid and the burden on the engineered bacteria. Therefore, we plan to divide the entire circuit into two parts, each constructed in different engineered bacteria, inserting new components to enable signal transmission between the two engineered bacterias.<!--]--></p><p><!--[-->Throughout the system, we have always planned to use the egfp gene as the reporter gene. However, this gene requires the presence of oxygen molecules as a cofactor for its luminescence, while the cultivation chamber of our designed hardware is in a low-oxygen environment. As a result, we tested a new reporter gene, BsFbFp, which can express significantly enhanced in vivo fluorescence in both aerobic and anaerobic environments, ensuring that the engineered bacteria can effectively express fluorescent signals in low-oxygen conditions.<!--]--></p><p><!--[-->Thus, the construction of the biological part of our circuit has been completed.<!--]--></p><h3 id="hardware"><!--[-->Hardware<!--]--></h3><p><!--[-->Point of Care Testing (POCT) is a form of rapid on-site testing/near-patient testing method which has been particularly impactful and is playing an increasingly vital role in the fields of medical diagnostics and environmental health monitoring due to its speed, convenience, and ease of operation. Traditional water contaminant detection technologies are often constrained by the need for costly equipment and a professional laboratory environment. In contrast, novel biosensor detection devices based on compact POCT equipment, when connected to an interactive app for operational control and visual data analysis, can effectively facilitate the rapid and efficient screening of emerging waterborne pollutants. This approach holds broad prospects for application in the field of environmental monitoring and assessment.<!--]--></p><h2 id="solution"><!--[-->Solution<!--]--></h2><h3 id="biology-1"><!--[-->biology<!--]--></h3><p><!--[-->To achieve signal communication between the two engineered bacteria, we studied the bacterial quorum sensing mechanism. This mechanism allows bacteria to exchange information and coordinate population behaviors when their numbers reach a certain density, regulating gene expression. When activated, bacteria secrete signal molecules that can freely diffuse across the cell membrane. Once the concentration of these signal molecules in the environment reaches a certain threshold, it induces the expression of specific genes in the bacteria. We introduced the LuxI/R system, where AHL (N-acyl homoserine lactone) is produced catalytically by the expression of the LuxI gene as a signaling molecule. This AHL freely diffuses into the second engineered bacteria and binds with the LuxR protein released by the activation of the LuxR gene, activating the P LuxR promoter to initiate downstream gene expression.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/description/biocircuit-p1.png 1x, https://static.igem.wiki/teams/5358/description/biocircuit-p1.png 2x" src="https://static.igem.wiki/teams/5358/description/biocircuit-p1.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/description/biocircuit-p2.png 1x, https://static.igem.wiki/teams/5358/description/biocircuit-p2.png 2x" src="https://static.igem.wiki/teams/5358/description/biocircuit-p2.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/description/biocircuit-p3.png 1x, https://static.igem.wiki/teams/5358/description/biocircuit-p3.png 2x" src="https://static.igem.wiki/teams/5358/description/biocircuit-p3.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/description/biocircuit-p4.png 1x, https://static.igem.wiki/teams/5358/description/biocircuit-p4.png 2x" src="https://static.igem.wiki/teams/5358/description/biocircuit-p4.png"><!--]--></p><h3 id="hardware-1"><!--[-->Hardware<!--]--></h3><p><!--[-->In response to the demands for standardized cultivation of engineered bacteria and high-sensitivity detection, we have designed a highly integrated and automated system that encompasses the entire process of bacterial strain revival, cultivation, detection, and analysis. The system utilizes an ESP32 chip as the main controller, interfaces with a temperature control system and a fluorescence detection system, employs a linear slide rail structure for high-throughput detection, and leverages a mobile app for automated device control and visualization of detection results. This system overcomes the limitations of time and space associated with traditional detection equipment, providing a versatile, portable, and highly sensitive solution for the detection of EDCs in aquatic environments across various scenarios.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/description/and-gate.png 1x, https://static.igem.wiki/teams/5358/description/and-gate.png 2x" src="https://static.igem.wiki/teams/5358/description/and-gate.png"><!--]--></p><h2 id="reference"><!--[--><em><!--[-->Reference<!--]--></em><!--]--></h2><div class="reference"><!--[--><ol><!--[--><li><!--[-->Cao Y, Li L, Shen KH, Liu JG. Disease burden attributable to endocrine-disrupting chemicals exposure in China: A case study of phthalates. Science of the Total Environment, 2019, Vol. 662(1): 615-621<!--]--></li><li><!--[-->Nematollah Asadi, Mahmoud Bahmani, Arash Kheradmand, Mahmoud Rafieian-Kopaei. The Impact of Oxidative Stress on Testicular Function and the Role of Antioxidants in Improving it: A Review. Journal of Clinical and Diagnostic Research, 2017, Vol. 11(5): IE1-IE5<!--]--></li><li><!--[-->Ma Ya, Liu Haohao, Wu Jinxia, Yuan Le, Wang Yueqin, Du Xingde, Wang Rui, Marwa Phelisters Wegesa, Petlulu Pavankumar, Chen Xinghai, Zhang Huizhen. The adverse health effects of bisphenol A and related toxicity mechanisms. Environmental Research, 2019, Vol. 176: 108<!--]--></li><li><!--[-->Liang R , Zhou J , Liu J. Construction of a Bacterial Assay for Estrogen Detection Based on an Estrogen-Sensitive Intein. Applied &amp; Environmental Microbiology, 2011, 77(7):2488.DOI:10.1128/AEM.02336-10.<!--]--></li><li><!--[-->Ma Ya, Liu Haohao, Wu Jinxia, Yuan Le, Wang Yueqin, Du Xingde, Wang Rui, Marwa, Phelisters Wegesa, Petlulu, Pavankumar, Chen Xinghai, Zhang Huizhen.The adverse health effects of bisphenol A and related toxicity mechanisms.Environmental Research, 2019, Vol.176: 108575<!--]--></li><li><!--[-->LIANG Jing-jia, GU Ai-hua. Multigenerational and cross-generational effect of environmental endocrine disruptors on reproductive system in male animals. <em><!--[-->Chinese Journal of Public Health<!--]--></em>, 2021, 37(2): 375-380.<!--]--></li><li><!--[-->Nematollah Asadi, Mahmoud Bahmani, Arash Kheradmand, Mahmoud Rafieian-Kopaei.The Impact of Oxidative Stress on Testicular Function and the Role of Antioxidants in Improving it: A Review.Journal of Clinical and Diagnostic Research, 2017, Vol.11(5): IE1-IE5<!--]--></li><li><!--[-->Mondal Shirsha, Ghosh Songita, Bhattacharya Samir, Mukherjee Sutapa. Chronic dietary administration of lower levels of diethyl phthalate induces murine testicular germ cell inflammation and sperm pathologies: Involvement of oxidative stress. Chemosphere, 2019, Vol.229: 443-451<!--]--></li><li><!--[-->Maslowska K H, Karolina Makiela‐Dzbenska, Fijalkowska I J. The SOS system: A complex and tightly regulated response to DNA damage. John Wiley &amp; Sons, Ltd, 2019(4). DOI:10.1002/em.22267.<!--]--></li><li><!--[-->Baojun W, Mauricio B, Martin B. Engineering modular and tunable genetic amplifiers for scaling transcriptional signals in cascaded gene networks. Nucleic Acids Research, 2014(14): 9484-9492. DOI:10.1093/nar/gku593.<!--]--></li><li><!--[-->Xiao Y, Zou H, Li J, Song T, Lv W, Wang W, Wang Z, Tao S. Impact of quorum sensing signaling molecules in gram-negative bacteria on host cells: current understanding and future perspectives. Gut Microbes. 2022 Jan-Dec, 14(1):2039048. doi: 10.1080/19490976.2022.2039048.<!--]--></li><li><!--[-->Drepper T, Eggert T, Circolone F, et al. Reporter proteins for in vivo fluorescence without oxygen. Nature Biotechnology, 2007, 25(4):443-445.DOI:10.1038/nbt1293.<!--]--></li><li><!--[-->Mukherjee A, Schroeder C M, Sikes H D, et al. Flavin-based fluorescent proteins: emerging paradigms in biological imaging. <em><!--[-->This review comes from a themed issue on Analytical biotechnology<!--]--></em>. 2015.<!--]--></li><!--]--></ol><!--]--></div></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a aria-current="page" href="/bit/description" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/description/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-Pcus8P4khp":11},null,"/description",1727871092285]</script>
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diff --git a/.output/public/education/_payload.json b/.output/public/education/_payload.json
index 3e7b9fe7dfe837d8bc800ba37d3264980071749a..80f3ca48e50b96712234c248a3ab0987f43ee5af 100644
--- a/.output/public/education/_payload.json
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diff --git a/.output/public/education/index.html b/.output/public/education/index.html
index 446c320e9b7005e8a52fc735af485cd616e74026..b1d70fe70001619948ccf65098b40d1def306049 100644
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.el-alert__description{color:var(--el-color-info)}.el-alert--info.is-dark{background-color:var(--el-color-info);color:var(--el-color-white)}.el-alert--warning{--el-alert-bg-color:var(--el-color-warning-light-9)}.el-alert--warning.is-light{background-color:var(--el-alert-bg-color)}.el-alert--warning.is-light,.el-alert--warning.is-light .el-alert__description{color:var(--el-color-warning)}.el-alert--warning.is-dark{background-color:var(--el-color-warning);color:var(--el-color-white)}.el-alert--error{--el-alert-bg-color:var(--el-color-error-light-9)}.el-alert--error.is-light{background-color:var(--el-alert-bg-color)}.el-alert--error.is-light,.el-alert--error.is-light .el-alert__description{color:var(--el-color-error)}.el-alert--error.is-dark{background-color:var(--el-color-error);color:var(--el-color-white)}.el-alert__content{display:flex;flex-direction:column;gap:4px}.el-alert .el-alert__icon{font-size:var(--el-alert-icon-size);margin-right:8px;width:var(--el-alert-icon-size)}.el-alert .el-alert__icon.is-big{font-size:var(--el-alert-icon-large-size);margin-right:12px;width:var(--el-alert-icon-large-size)}.el-alert__title{font-size:var(--el-alert-title-font-size);line-height:24px}.el-alert__title.with-description{font-size:var(--el-alert-title-with-description-font-size)}.el-alert .el-alert__description{font-size:var(--el-alert-description-font-size);margin:0}.el-alert .el-alert__close-btn{cursor:pointer;font-size:var(--el-alert-close-font-size);opacity:1;position:absolute;right:16px;top:12px}.el-alert .el-alert__close-btn.is-customed{font-size:var(--el-alert-close-customed-font-size);font-style:normal;line-height:24px;top:8px}.el-alert-fade-enter-from,.el-alert-fade-leave-active{opacity:0}.el-aside{box-sizing:border-box;flex-shrink:0;overflow:auto;width:var(--el-aside-width,300px)}.el-autocomplete{--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;display:inline-block;position:relative;width:var(--el-input-width)}.el-autocomplete__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-autocomplete__popper.el-popper,.el-autocomplete__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-border-color-light)}.el-autocomplete__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-autocomplete__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-autocomplete__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-autocomplete__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-autocomplete-suggestion{border-radius:var(--el-border-radius-base);box-sizing:border-box}.el-autocomplete-suggestion__wrap{box-sizing:border-box;max-height:280px;padding:10px 0}.el-autocomplete-suggestion__list{margin:0;padding:0}.el-autocomplete-suggestion li{color:var(--el-text-color-regular);cursor:pointer;font-size:var(--el-font-size-base);line-height:34px;list-style:none;margin:0;overflow:hidden;padding:0 20px;text-align:left;text-overflow:ellipsis;white-space:nowrap}.el-autocomplete-suggestion li.highlighted,.el-autocomplete-suggestion li:hover{background-color:var(--el-fill-color-light)}.el-autocomplete-suggestion li.divider{border-top:1px solid var(--el-color-black);margin-top:6px}.el-autocomplete-suggestion li.divider:last-child{margin-bottom:-6px}.el-autocomplete-suggestion.is-loading li{color:var(--el-text-color-secondary);font-size:20px;height:100px;line-height:100px;text-align:center}.el-autocomplete-suggestion.is-loading li:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-autocomplete-suggestion.is-loading li:hover{background-color:var(--el-bg-color-overlay)}.el-autocomplete-suggestion.is-loading .el-icon-loading{vertical-align:middle}.el-avatar{--el-avatar-text-color:var(--el-color-white);--el-avatar-bg-color:var(--el-text-color-disabled);--el-avatar-text-size:14px;--el-avatar-icon-size:18px;--el-avatar-border-radius:var(--el-border-radius-base);--el-avatar-size-large:56px;--el-avatar-size-small:24px;--el-avatar-size:40px;align-items:center;background:var(--el-avatar-bg-color);box-sizing:border-box;color:var(--el-avatar-text-color);display:inline-flex;font-size:var(--el-avatar-text-size);height:var(--el-avatar-size);justify-content:center;outline:none;overflow:hidden;text-align:center;width:var(--el-avatar-size)}.el-avatar>img{display:block;height:100%;width:100%}.el-avatar--circle{border-radius:50%}.el-avatar--square{border-radius:var(--el-avatar-border-radius)}.el-avatar--icon{font-size:var(--el-avatar-icon-size)}.el-avatar--small{--el-avatar-size:24px}.el-avatar--large{--el-avatar-size:56px}.el-backtop{--el-backtop-bg-color:var(--el-bg-color-overlay);--el-backtop-text-color:var(--el-color-primary);--el-backtop-hover-bg-color:var(--el-border-color-extra-light);align-items:center;background-color:var(--el-backtop-bg-color);border-radius:50%;box-shadow:var(--el-box-shadow-lighter);color:var(--el-backtop-text-color);cursor:pointer;display:flex;font-size:20px;height:40px;justify-content:center;position:fixed;width:40px;z-index:5}.el-backtop:hover{background-color:var(--el-backtop-hover-bg-color)}.el-backtop__icon{font-size:20px}.el-badge{--el-badge-bg-color:var(--el-color-danger);--el-badge-radius:10px;--el-badge-font-size:12px;--el-badge-padding:6px;--el-badge-size:18px;display:inline-block;position:relative;vertical-align:middle;width:-moz-fit-content;width:fit-content}.el-badge__content{align-items:center;background-color:var(--el-badge-bg-color);border:1px solid var(--el-bg-color);border-radius:var(--el-badge-radius);color:var(--el-color-white);display:inline-flex;font-size:var(--el-badge-font-size);height:var(--el-badge-size);justify-content:center;padding:0 var(--el-badge-padding);white-space:nowrap}.el-badge__content.is-fixed{position:absolute;right:calc(1px + var(--el-badge-size)/2);top:0;transform:translateY(-50%) translateX(100%);z-index:var(--el-index-normal)}.el-badge__content.is-fixed.is-dot{right:5px}.el-badge__content.is-dot{border-radius:50%;height:8px;padding:0;right:0;width:8px}.el-badge__content--primary{background-color:var(--el-color-primary)}.el-badge__content--success{background-color:var(--el-color-success)}.el-badge__content--warning{background-color:var(--el-color-warning)}.el-badge__content--info{background-color:var(--el-color-info)}.el-badge__content--danger{background-color:var(--el-color-danger)}.el-breadcrumb{font-size:14px;line-height:1}.el-breadcrumb:after,.el-breadcrumb:before{content:"";display:table}.el-breadcrumb:after{clear:both}.el-breadcrumb__separator{color:var(--el-text-color-placeholder);font-weight:700;margin:0 9px}.el-breadcrumb__separator.el-icon{font-weight:400;margin:0 6px}.el-breadcrumb__separator.el-icon svg{vertical-align:middle}.el-breadcrumb__item{align-items:center;display:inline-flex;float:left}.el-breadcrumb__inner{color:var(--el-text-color-regular)}.el-breadcrumb__inner a,.el-breadcrumb__inner.is-link{color:var(--el-text-color-primary);font-weight:700;text-decoration:none;transition:var(--el-transition-color)}.el-breadcrumb__inner a:hover,.el-breadcrumb__inner.is-link:hover{color:var(--el-color-primary);cursor:pointer}.el-breadcrumb__item:last-child .el-breadcrumb__inner,.el-breadcrumb__item:last-child .el-breadcrumb__inner a,.el-breadcrumb__item:last-child .el-breadcrumb__inner a:hover,.el-breadcrumb__item:last-child .el-breadcrumb__inner:hover{color:var(--el-text-color-regular);cursor:text;font-weight:400}.el-breadcrumb__item:last-child .el-breadcrumb__separator{display:none}.el-button-group{display:inline-block;vertical-align:middle}.el-button-group:after,.el-button-group:before{content:"";display:table}.el-button-group:after{clear:both}.el-button-group>.el-button{float:left;position:relative}.el-button-group>.el-button+.el-button{margin-left:0}.el-button-group>.el-button:first-child{border-bottom-right-radius:0;border-top-right-radius:0}.el-button-group>.el-button:last-child{border-bottom-left-radius:0;border-top-left-radius:0}.el-button-group>.el-button:first-child:last-child{border-bottom-left-radius:var(--el-border-radius-base);border-bottom-right-radius:var(--el-border-radius-base);border-top-left-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-button-group>.el-button:first-child:last-child.is-round{border-radius:var(--el-border-radius-round)}.el-button-group>.el-button:first-child:last-child.is-circle{border-radius:50%}.el-button-group>.el-button:not(:first-child):not(:last-child){border-radius:0}.el-button-group>.el-button:not(:last-child){margin-right:-1px}.el-button-group>.el-button.is-active,.el-button-group>.el-button:active,.el-button-group>.el-button:focus,.el-button-group>.el-button:hover{z-index:1}.el-button-group>.el-dropdown>.el-button{border-bottom-left-radius:0;border-left-color:var(--el-button-divide-border-color);border-top-left-radius:0}.el-button-group .el-button--primary:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--primary:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--primary:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--success:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--success:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--success:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--warning:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--warning:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--warning:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--danger:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--danger:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--danger:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--info:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--info:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--info:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button{--el-button-font-weight:var(--el-font-weight-primary);--el-button-border-color:var(--el-border-color);--el-button-bg-color:var(--el-fill-color-blank);--el-button-text-color:var(--el-text-color-regular);--el-button-disabled-text-color:var(--el-disabled-text-color);--el-button-disabled-bg-color:var(--el-fill-color-blank);--el-button-disabled-border-color:var(--el-border-color-light);--el-button-divide-border-color:hsla(0,0%,100%,.5);--el-button-hover-text-color:var(--el-color-primary);--el-button-hover-bg-color:var(--el-color-primary-light-9);--el-button-hover-border-color:var(--el-color-primary-light-7);--el-button-active-text-color:var(--el-button-hover-text-color);--el-button-active-border-color:var(--el-color-primary);--el-button-active-bg-color:var(--el-button-hover-bg-color);--el-button-outline-color:var(--el-color-primary-light-5);--el-button-hover-link-text-color:var(--el-color-info);--el-button-active-color:var(--el-text-color-primary);align-items:center;-webkit-appearance:none;background-color:var(--el-button-bg-color);border:var(--el-border);border-color:var(--el-button-border-color);box-sizing:border-box;color:var(--el-button-text-color);cursor:pointer;display:inline-flex;font-weight:var(--el-button-font-weight);height:32px;justify-content:center;line-height:1;outline:none;text-align:center;transition:.1s;-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-button:hover{background-color:var(--el-button-hover-bg-color);border-color:var(--el-button-hover-border-color);color:var(--el-button-hover-text-color);outline:none}.el-button:active{background-color:var(--el-button-active-bg-color);border-color:var(--el-button-active-border-color);color:var(--el-button-active-text-color);outline:none}.el-button:focus-visible{outline:2px solid var(--el-button-outline-color);outline-offset:1px;transition:outline-offset 0s,outline 0s}.el-button>span{align-items:center;display:inline-flex}.el-button+.el-button{margin-left:12px}.el-button{border-radius:var(--el-border-radius-base);font-size:var(--el-font-size-base)}.el-button,.el-button.is-round{padding:8px 15px}.el-button::-moz-focus-inner{border:0}.el-button [class*=el-icon]+span{margin-left:6px}.el-button [class*=el-icon] 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20px;position:relative}.el-cascader-node.is-selectable.in-active-path{color:var(--el-cascader-menu-text-color)}.el-cascader-node.in-active-path,.el-cascader-node.is-active,.el-cascader-node.is-selectable.in-checked-path{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader-node:not(.is-disabled){cursor:pointer}.el-cascader-node:not(.is-disabled):focus,.el-cascader-node:not(.is-disabled):hover{background:var(--el-cascader-node-background-hover)}.el-cascader-node.is-disabled{color:var(--el-cascader-node-color-disabled);cursor:not-allowed}.el-cascader-node__prefix{left:10px;position:absolute}.el-cascader-node__postfix{position:absolute;right:10px}.el-cascader-node__label{flex:1;overflow:hidden;padding:0 8px;text-align:left;text-overflow:ellipsis;white-space:nowrap}.el-cascader-node>.el-checkbox,.el-cascader-node>.el-radio{margin-right:0}.el-cascader-node>.el-radio .el-radio__label{padding-left:0}.el-cascader{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);display:inline-block;font-size:var(--el-font-size-base);line-height:32px;outline:none;position:relative;vertical-align:middle}.el-cascader:not(.is-disabled):hover .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset;cursor:pointer}.el-cascader .el-input{cursor:pointer;display:flex}.el-cascader .el-input .el-input__inner{cursor:pointer;text-overflow:ellipsis}.el-cascader .el-input .el-input__suffix-inner .el-icon{height:calc(100% - 2px)}.el-cascader .el-input .el-input__suffix-inner .el-icon svg{vertical-align:middle}.el-cascader .el-input .icon-arrow-down{font-size:14px;transition:transform var(--el-transition-duration)}.el-cascader .el-input .icon-arrow-down.is-reverse{transform:rotate(180deg)}.el-cascader .el-input .icon-circle-close:hover{color:var(--el-input-clear-hover-color,var(--el-text-color-secondary))}.el-cascader .el-input.is-focus .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-focus-border-color,var(--el-color-primary)) inset}.el-cascader--large{font-size:14px;line-height:40px}.el-cascader--small{font-size:12px;line-height:24px}.el-cascader.is-disabled .el-cascader__label{color:var(--el-disabled-text-color);z-index:calc(var(--el-index-normal) + 1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper .el-popper__arrow:before{border:var(--el-cascader-menu-border)}.el-cascader__dropdown.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper{box-shadow:var(--el-cascader-menu-shadow)}.el-cascader__tags{box-sizing:border-box;display:flex;flex-wrap:wrap;left:0;line-height:normal;position:absolute;right:30px;text-align:left;top:50%;transform:translateY(-50%)}.el-cascader__tags .el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags .el-tag{align-items:center;background:var(--el-fill-color);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag.el-tag--dark,.el-cascader__collapse-tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__collapse-tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__collapse-tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__collapse-tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__suggestion-panel{border-radius:var(--el-cascader-menu-radius)}.el-cascader__suggestion-list{color:var(--el-cascader-menu-text-color);font-size:var(--el-font-size-base);margin:0;max-height:204px;padding:6px 0;text-align:center}.el-cascader__suggestion-item{align-items:center;cursor:pointer;display:flex;height:34px;justify-content:space-between;outline:none;padding:0 15px;text-align:left}.el-cascader__suggestion-item:focus,.el-cascader__suggestion-item:hover{background:var(--el-cascader-node-background-hover)}.el-cascader__suggestion-item.is-checked{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader__suggestion-item>span{margin-right:10px}.el-cascader__empty-text{color:var(--el-cascader-color-empty);margin:10px 0}.el-cascader__search-input{background:transparent;border:none;box-sizing:border-box;color:var(--el-cascader-menu-text-color);flex:1;height:24px;margin:2px 0 2px 11px;min-width:60px;outline:none;padding:0}.el-cascader__search-input::-moz-placeholder{color:transparent}.el-cascader__search-input::placeholder{color:transparent}.el-check-tag{background-color:var(--el-color-info-light-9);border-radius:var(--el-border-radius-base);color:var(--el-color-info);cursor:pointer;display:inline-block;font-size:var(--el-font-size-base);font-weight:700;line-height:var(--el-font-size-base);padding:7px 15px;transition:var(--el-transition-all)}.el-check-tag:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--primary.is-checked{background-color:var(--el-color-primary-light-8);color:var(--el-color-primary)}.el-check-tag.el-check-tag--primary.is-checked:hover{background-color:var(--el-color-primary-light-7)}.el-check-tag.el-check-tag--primary.is-checked.is-disabled{background-color:var(--el-color-primary-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-checked.is-disabled:hover{background-color:var(--el-color-primary-light-8)}.el-check-tag.el-check-tag--primary.is-disabled{background-color:var(--el-color-info-light-9);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-check-tag.el-check-tag--success.is-checked{background-color:var(--el-color-success-light-8);color:var(--el-color-success)}.el-check-tag.el-check-tag--success.is-checked:hover{background-color:var(--el-color-success-light-7)}.el-check-tag.el-check-tag--success.is-checked.is-disabled{background-color:var(--el-color-success-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-checked.is-disabled:hover{background-color:var(--el-color-success-light-8)}.el-check-tag.el-check-tag--success.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-disabled,.el-check-tag.el-check-tag--success.is-disabled:hover{background-color:var(--el-color-success-light-9)}.el-check-tag.el-check-tag--warning.is-checked{background-color:var(--el-color-warning-light-8);color:var(--el-color-warning)}.el-check-tag.el-check-tag--warning.is-checked:hover{background-color:var(--el-color-warning-light-7)}.el-check-tag.el-check-tag--warning.is-checked.is-disabled{background-color:var(--el-color-warning-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-checked.is-disabled:hover{background-color:var(--el-color-warning-light-8)}.el-check-tag.el-check-tag--warning.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-disabled,.el-check-tag.el-check-tag--warning.is-disabled:hover{background-color:var(--el-color-warning-light-9)}.el-check-tag.el-check-tag--danger.is-checked{background-color:var(--el-color-danger-light-8);color:var(--el-color-danger)}.el-check-tag.el-check-tag--danger.is-checked:hover{background-color:var(--el-color-danger-light-7)}.el-check-tag.el-check-tag--danger.is-checked.is-disabled{background-color:var(--el-color-danger-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-checked.is-disabled:hover{background-color:var(--el-color-danger-light-8)}.el-check-tag.el-check-tag--danger.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-disabled,.el-check-tag.el-check-tag--danger.is-disabled:hover{background-color:var(--el-color-danger-light-9)}.el-check-tag.el-check-tag--error.is-checked{background-color:var(--el-color-error-light-8);color:var(--el-color-error)}.el-check-tag.el-check-tag--error.is-checked:hover{background-color:var(--el-color-error-light-7)}.el-check-tag.el-check-tag--error.is-checked.is-disabled{background-color:var(--el-color-error-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-checked.is-disabled:hover{background-color:var(--el-color-error-light-8)}.el-check-tag.el-check-tag--error.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-disabled,.el-check-tag.el-check-tag--error.is-disabled:hover{background-color:var(--el-color-error-light-9)}.el-check-tag.el-check-tag--info.is-checked{background-color:var(--el-color-info-light-8);color:var(--el-color-info)}.el-check-tag.el-check-tag--info.is-checked:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--info.is-checked.is-disabled{background-color:var(--el-color-info-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-checked.is-disabled:hover{background-color:var(--el-color-info-light-8)}.el-check-tag.el-check-tag--info.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-disabled,.el-check-tag.el-check-tag--info.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-checkbox-button{--el-checkbox-button-checked-bg-color:var(--el-color-primary);--el-checkbox-button-checked-text-color:var(--el-color-white);--el-checkbox-button-checked-border-color:var(--el-color-primary);display:inline-block;position:relative}.el-checkbox-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left-color:transparent;bord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15px;position:relative;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-checkbox-button__inner.is-round{padding:8px 15px}.el-checkbox-button__inner:hover{color:var(--el-color-primary)}.el-checkbox-button__inner [class*=el-icon-]{line-height:.9}.el-checkbox-button__inner [class*=el-icon-]+span{margin-left:5px}.el-checkbox-button__original{margin:0;opacity:0;outline:none;position:absolute;z-index:-1}.el-checkbox-button.is-checked .el-checkbox-button__inner{background-color:var(--el-checkbox-button-checked-bg-color);border-color:var(--el-checkbox-button-checked-border-color);box-shadow:-1px 0 0 0 var(--el-color-primary-light-7);color:var(--el-checkbox-button-checked-text-color)}.el-checkbox-button.is-checked:first-child .el-checkbox-button__inner{border-left-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button.is-disabled .el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large .el-checkbox-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-checkbox-button--large .el-checkbox-button__inner.is-round{padding:12px 19px}.el-checkbox-button--small .el-checkbox-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-checkbox-button--small .el-checkbox-button__inner.is-round{padding:5px 11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) .el-checkbox__original:not(:focus-visible){border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__input.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-checked-icon-color);content:"";display:block;height:2px;left:0;position:absolute;right:0;top:5px;transform:scale(.5)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:after{display:none}.el-checkbox__inner{background-color:var(--el-checkbox-bg-color);border:var(--el-checkbox-input-border);border-radius:var(--el-checkbox-border-radius);box-sizing:border-box;display:inline-block;height:var(--el-checkbox-input-height);position:relative;transition:border-color .25s cubic-bezier(.71,-.46,.29,1.46),background-color .25s cubic-bezier(.71,-.46,.29,1.46),outline .25s cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 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54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 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var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid 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var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker .el-picker-panel__content{margin:0}.el-date-range-picker__header{height:28px;position:relative;text-align:center}.el-date-range-picker__header [class*=arrow-left]{float:left}.el-date-range-picker__header [class*=arrow-right]{float:right}.el-date-range-picker__header div{font-size:16px;font-weight:500;margin-right:50px}.el-date-range-picker__content{box-sizing:border-box;float:left;margin:0;padding:16px;width:50%}.el-date-range-picker__content.is-left{border-right:1px solid var(--el-datepicker-inner-border-color)}.el-date-range-picker__content .el-date-range-picker__header div{margin-left:50px;margin-right:50px}.el-date-range-picker__editors-wrap{box-sizing:border-box;display:table-cell}.el-date-range-picker__editors-wrap.is-right{text-align:right}.el-date-range-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid var(--el-timepicker-inner-border-color,var(--el-border-color-light));box-sizing:border-box;height:36px;line-height:25px;padding:4px;text-align:right}.el-time-panel__btn{background-color:transparent;border:none;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:28px;margin:0 5px;outline:none;padding:0 5px}.el-time-panel__btn.confirm{color:var(--el-timepicker-active-color,var(--el-color-primary));font-weight:800}.el-descriptions{--el-descriptions-table-border:1px solid var(--el-border-color-lighter);--el-descriptions-item-bordered-label-background:var(--el-fill-color-light);box-sizing:border-box;color:var(--el-text-color-primary);font-size:var(--el-font-size-base)}.el-descriptions__header{align-items:center;display:flex;justify-content:space-between;margin-bottom:16px}.el-descriptions__title{color:var(--el-text-color-primary);font-size:16px;font-weight:700}.el-descriptions__body{background-color:var(--el-fill-color-blank)}.el-descriptions__body 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var(--el-menu-base-level-padding)}.horizontal-collapse-transition .el-sub-menu__title 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.el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small .el-radio__inner{height:12px;width:12px}.el-rate{--el-rate-height:20px;--el-rate-font-size:var(--el-font-size-base);--el-rate-icon-size:18px;--el-rate-icon-margin:6px;--el-rate-void-color:var(--el-border-color-darker);--el-rate-fill-color:#f7ba2a;--el-rate-disabled-void-color:var(--el-fill-color);--el-rate-text-color:var(--el-text-color-primary);align-items:center;display:inline-flex;height:32px}.el-rate:active,.el-rate:focus{outline:none}.el-rate__item{color:var(--el-rate-void-color);cursor:pointer;display:inline-block;font-size:0;line-height:normal;position:relative;vertical-align:middle}.el-rate .el-rate__icon{display:inline-block;font-size:var(--el-rate-icon-size);margin-right:var(--el-rate-icon-margin);position:relative;transition:var(--el-transition-duration)}.el-rate .el-rate__icon.hover{transform:scale(1.15)}.el-rate .el-rate__icon .path2{left:0;position:absolute;top:0}.el-rate .el-rate__icon.is-active{color:var(--el-rate-fill-color)}.el-rate__decimal{color:var(--el-rate-fill-color);display:inline-block;overflow:hidden}.el-rate__decimal,.el-rate__decimal--box{left:0;position:absolute;top:0}.el-rate__text{color:var(--el-rate-text-color);font-size:var(--el-rate-font-size);vertical-align:middle}.el-rate--large{height:40px}.el-rate--small{height:24px}.el-rate--small .el-rate__icon{font-size:14px}.el-rate.is-disabled .el-rate__item{color:var(--el-rate-disabled-void-color);cursor:auto}.el-result{--el-result-padding:40px 30px;--el-result-icon-font-size:64px;--el-result-title-font-size:20px;--el-result-title-margin-top:20px;--el-result-subtitle-margin-top:10px;--el-result-extra-margin-top:30px;align-items:center;box-sizing:border-box;display:flex;flex-direction:column;justify-content:center;padding:var(--el-result-padding);text-align:center}.el-result__icon svg{height:var(--el-result-icon-font-size);width:var(--el-result-icon-font-size)}.el-result__title{margin-top:var(--el-result-title-margin-top)}.el-result__title p{color:var(--el-text-color-primary);font-size:var(--el-result-title-font-size);line-height:1.3;margin:0}.el-result__subtitle{margin-top:var(--el-result-subtitle-margin-top)}.el-result__subtitle p{color:var(--el-text-color-regular);font-size:var(--el-font-size-base);line-height:1.3;margin:0}.el-result__extra{margin-top:var(--el-result-extra-margin-top)}.el-result .icon-primary{--el-result-color:var(--el-color-primary);color:var(--el-result-color)}.el-result .icon-success{--el-result-color:var(--el-color-success);color:var(--el-result-color)}.el-result .icon-warning{--el-result-color:var(--el-color-warning);color:var(--el-result-color)}.el-result .icon-danger{--el-result-color:var(--el-color-danger);color:var(--el-result-color)}.el-result .icon-error{--el-result-color:var(--el-color-error);color:var(--el-result-color)}.el-result 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.el-tag__close{color:var(--el-tag-text-color);flex-shrink:0}.el-tag .el-tag__close:hover{background-color:var(--el-tag-hover-color);color:var(--el-color-white)}.el-tag.el-tag--primary{--el-tag-text-color:var(--el-color-primary)}.el-tag.el-tag--success{--el-tag-text-color:var(--el-color-success)}.el-tag.el-tag--warning{--el-tag-text-color:var(--el-color-warning)}.el-tag.el-tag--danger{--el-tag-text-color:var(--el-color-danger)}.el-tag.el-tag--error{--el-tag-text-color:var(--el-color-error)}.el-tag.el-tag--info{--el-tag-text-color:var(--el-color-info)}.el-tag .el-icon{border-radius:50%;cursor:pointer;font-size:calc(var(--el-icon-size) - 2px);height:var(--el-icon-size);width:var(--el-icon-size)}.el-tag 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0;min-width:0}.time-select .el-picker-panel__content{margin:0;max-height:200px}.time-select-item{font-size:14px;line-height:20px;padding:8px 10px}.time-select-item.disabled{color:var(--el-datepicker-border-color);cursor:not-allowed}.time-select-item:hover{background-color:var(--el-fill-color-light);cursor:pointer;font-weight:700}.time-select .time-select-item.selected:not(.disabled){color:var(--el-color-primary);font-weight:700}.el-timeline-item{padding-bottom:20px;position:relative}.el-timeline-item__wrapper{padding-left:28px;position:relative;top:-3px}.el-timeline-item__tail{border-left:2px solid var(--el-timeline-node-color);height:100%;left:4px;position:absolute}.el-timeline-item .el-timeline-item__icon{color:var(--el-color-white);font-size:var(--el-font-size-small)}.el-timeline-item__node{align-items:center;background-color:var(--el-timeline-node-color);border-color:var(--el-timeline-node-color);border-radius:50%;box-sizing:border-box;display:flex;justify-content:center;position:absolute}.el-timeline-item__node--normal{height:var(--el-timeline-node-size-normal);left:-1px;width:var(--el-timeline-node-size-normal)}.el-timeline-item__node--large{height:var(--el-timeline-node-size-large);left:-2px;width:var(--el-timeline-node-size-large)}.el-timeline-item__node.is-hollow{background:var(--el-color-white);border-style:solid;border-width:2px}.el-timeline-item__node--primary{background-color:var(--el-color-primary);border-color:var(--el-color-primary)}.el-timeline-item__node--success{background-color:var(--el-color-success);border-color:var(--el-color-success)}.el-timeline-item__node--warning{background-color:var(--el-color-warning);border-color:var(--el-color-warning)}.el-timeline-item__node--danger{background-color:var(--el-color-danger);border-color:var(--el-color-danger)}.el-timeline-item__node--info{background-color:var(--el-color-info);border-color:var(--el-color-info)}.el-timeline-item__dot{align-items:center;display:flex;justify-content:center;position:absolute}.el-timeline-item__content{color:var(--el-text-color-primary)}.el-timeline-item__timestamp{color:var(--el-text-color-secondary);font-size:var(--el-font-size-small);line-height:1}.el-timeline-item__timestamp.is-top{margin-bottom:8px;padding-top:4px}.el-timeline-item__timestamp.is-bottom{margin-top:8px}.el-timeline{--el-timeline-node-size-normal:12px;--el-timeline-node-size-large:14px;--el-timeline-node-color:var(--el-border-color-light);font-size:var(--el-font-size-base);list-style:none;margin:0}.el-timeline 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srcset="https://static.igem.wiki/teams/5358/banner/hp.webp 1x, https://static.igem.wiki/teams/5358/banner/hp.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/hp.webp" data-v-ea72ee37><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg" data-v-ea72ee37>Education - More accessible knowledge </div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10" data-v-ea72ee37><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[500px] md:mr-10" data-v-ea72ee37><h1 data-v-ea72ee37>TOC</h1><ul data-v-ea72ee37><!--[--><!--]--></ul></aside><div class="content" data-v-ea72ee37><div class="markdown-content" data-v-ea72ee37><p data-v-ea72ee37> The core of education is to flexibly adjust the form and content of spreading knowledge for different groups of people, making the core of iGEM and synthetic biology accessible to different audiences. After clarifying the practical significance of the project, we hope to spread the necessity and effect of our project to audiences with different knowledge levels, so that more people can feel the charm of synthetic biology, and provide feedback from multiple angles and aspects. We conducted a series of face-to-face propaganda activities, and adjusted the publicity methods to achieve the best publicity effect in four different groups: students in rural areas, middle school students in urban areas, college students and social groups. </p><h2 id="rural-area" data-v-ea72ee37>Students in rural area - Textbook guided, easily acquired</h2><p data-v-ea72ee37> In Fangshan County, Shanxi Province, which is the counterpart of BIT, students can only acquire biological knowledge through textbooks. Starting from textbook knowledge such as genetic central dogma and genetic engineering, we connect synthetic biology with high school textbook knowledge to help students grasp the basic content and connotation of synthetic biology more easily. </p><!----><h2 id="urban-students" data-v-ea72ee37>Urban students - From appearance to essence</h2><p data-v-ea72ee37> Many middle schools in Beijing have a deep understanding of synthetic biology, and many schools have formed iGEM teams. We go to the High School Attached to Renmin University of China, Beijing No.5 High School and other schools to introduce relevant knowledge and show our projects to students who are passionate about synthetic biology, hoping to stimulate their enthusiasm and inspire them to apply synthetic biology principles to solve real-world problems. </p><!----><p data-v-ea72ee37> At the same time, we also communicated with many iGEM teams in high schools to discuss how to improve the biosecurity of the line by means of cell suicide, how to optimize the questionnaire, and how to improve the efficiency and reliability of the investigation.Finally, we review this year&#39;s work, hoping to reach cooperation in the future </p><!----><h2 id="college-students" data-v-ea72ee37>College students - collision of ideas</h2><p data-v-ea72ee37> Together with iGEM teams from Peking University, Shandong University and University of Electronic Science and Technology of China, we jointly launched the &quot;University Linkage: Unlocking the Mysteries of Synthetic Biology&quot; popular science Twitter campaign. At the same time, the offline sharing session discussed the feasibility, safety and ethics of synthetic biology in human health, environmental protection and sustainable development with students from different professional backgrounds, and got a lot of valuable and meaningful feedback. These processes inspired us to think about the value of iGEM and its future applications. </p><!----><h2 id="social-groups" data-v-ea72ee37>Social groups - Design a board game, make language interesting</h2><p data-v-ea72ee37> In order to make the charm of synthetic biology accessible to all social groups of different ages and cognitive levels, we collaborated with Beijing Dingshi Middle School to design a board game - POKEY-MON. In the game, players will win by collecting cards and in the process can boost themselves or interfere with others. During the design process, we repeatedly listened to the opinions of various parties, and once launched, it was widely praised. </p><!----><div class="flex justify-center" data-v-ea72ee37><iframe src="https://static.igem.wiki/teams/5358/education/pokey.pdf" width="100%" class="pdf-viewer h-[50vh]" title="POKEY-MON Board Game PDF" data-v-ea72ee37></iframe></div><p data-v-ea72ee37> Together with iGEM teams from Lanzhou University, Jilin University, Nanjing University of Technology, Sichuan University, Hainan University, Zhejiang University of Technology and other well-known Chinese universities, we launched a sign language video to introduce our project in order to expand our audience. 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tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><div class="el-menu--vertical el-menu--popup-container"><ul class="el-menu el-menu--popup el-menu--popup-right-start" style="--el-menu-level:1;"><!--[--><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Members<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Attributions<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Contribution<!--]--><!--[--><!--]--><!--]--></li><!--]--></ul></div><!--]--><!--v-if--><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2011;position:absolute;left:0;top:0;display:none;" class="el-popper is-pure is-light" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><div class="el-menu--vertical el-menu--popup-container"><ul class="el-menu el-menu--popup el-menu--popup-right-start" 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data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 2x" class="cursor-pointer py-2" src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div class="grow"></div><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->HOME<!--]--><!--[--><!--]--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Project<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Wet Lab<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Dry Lab<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Team<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Human Practices<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li></ul><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-mobile px-10 flex lg:hidden"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 2x" class="cursor-pointer py-2" src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div class="grow"></div><!--[--><span class="el-avatar el-avatar--icon el-avatar--circle self-center cursor-pointer el-tooltip__trigger el-tooltip__trigger" style=""><i class="el-icon" style=""><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M160 448a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zM160 896a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32z"></path></svg><!--]--></i></span><!--teleport start--><!--teleport end--><!--]--></ul></header><div class="pt-[8rem]"><!--[--><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="cover" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/hp.webp 1x, https://static.igem.wiki/teams/5358/banner/hp.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/hp.webp" data-v-ea72ee37><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg" data-v-ea72ee37>Education - More accessible knowledge </div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10" data-v-ea72ee37><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[500px] md:mr-10" data-v-ea72ee37><h1 data-v-ea72ee37>TOC</h1><ul data-v-ea72ee37><!--[--><!--]--></ul></aside><div class="content" data-v-ea72ee37><div class="markdown-content" data-v-ea72ee37><p data-v-ea72ee37> The core of education is to flexibly adjust the form and content of spreading knowledge for different groups of people, making the core of iGEM and synthetic biology accessible to different audiences. After clarifying the practical significance of the project, we hope to spread the necessity and effect of our project to audiences with different knowledge levels, so that more people can feel the charm of synthetic biology, and provide feedback from multiple angles and aspects. We conducted a series of face-to-face propaganda activities, and adjusted the publicity methods to achieve the best publicity effect in four different groups: students in rural areas, middle school students in urban areas, college students and social groups. </p><h2 id="rural-area" data-v-ea72ee37>Students in rural area - Textbook guided, easily acquired</h2><p data-v-ea72ee37> In Fangshan County, Shanxi Province, which is the counterpart of BIT, students can only acquire biological knowledge through textbooks. Starting from textbook knowledge such as genetic central dogma and genetic engineering, we connect synthetic biology with high school textbook knowledge to help students grasp the basic content and connotation of synthetic biology more easily. </p><!----><h2 id="urban-students" data-v-ea72ee37>Urban students - From appearance to essence</h2><p data-v-ea72ee37> Many middle schools in Beijing have a deep understanding of synthetic biology, and many schools have formed iGEM teams. We go to the High School Attached to Renmin University of China, Beijing No.5 High School and other schools to introduce relevant knowledge and show our projects to students who are passionate about synthetic biology, hoping to stimulate their enthusiasm and inspire them to apply synthetic biology principles to solve real-world problems. </p><!----><p data-v-ea72ee37> At the same time, we also communicated with many iGEM teams in high schools to discuss how to improve the biosecurity of the line by means of cell suicide, how to optimize the questionnaire, and how to improve the efficiency and reliability of the investigation.Finally, we review this year&#39;s work, hoping to reach cooperation in the future </p><!----><h2 id="college-students" data-v-ea72ee37>College students - collision of ideas</h2><p data-v-ea72ee37> Together with iGEM teams from Peking University, Shandong University and University of Electronic Science and Technology of China, we jointly launched the &quot;University Linkage: Unlocking the Mysteries of Synthetic Biology&quot; popular science Twitter campaign. At the same time, the offline sharing session discussed the feasibility, safety and ethics of synthetic biology in human health, environmental protection and sustainable development with students from different professional backgrounds, and got a lot of valuable and meaningful feedback. These processes inspired us to think about the value of iGEM and its future applications. </p><!----><h2 id="social-groups" data-v-ea72ee37>Social groups - Design a board game, make language interesting</h2><p data-v-ea72ee37> In order to make the charm of synthetic biology accessible to all social groups of different ages and cognitive levels, we collaborated with Beijing Dingshi Middle School to design a board game - POKEY-MON. In the game, players will win by collecting cards and in the process can boost themselves or interfere with others. During the design process, we repeatedly listened to the opinions of various parties, and once launched, it was widely praised. </p><!----><div class="flex justify-center" data-v-ea72ee37><iframe src="https://static.igem.wiki/teams/5358/education/pokey.pdf" width="100%" class="pdf-viewer h-[50vh]" title="POKEY-MON Board Game PDF" data-v-ea72ee37></iframe></div><p data-v-ea72ee37> Together with iGEM teams from Lanzhou University, Jilin University, Nanjing University of Technology, Sichuan University, Hainan University, Zhejiang University of Technology and other well-known Chinese universities, we launched a sign language video to introduce our project in order to expand our audience. It is hoped that through this video, more groups, including the hearing impaired, can learn about synthetic biology and iGEM, and remove the barriers between them and knowledge. </p><!----></div></div></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a aria-current="page" href="/bit/education" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/education/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":11,"prerenderedAt":12},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{},"/education",1727871091263]</script>
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diff --git a/.output/public/engineering/_payload.json b/.output/public/engineering/_payload.json
index bf0d654c9237b1b26d83629e1ef08e1667111cfd..7108fe1798034a7ec258b312d3b86fa6e8b94841 100644
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+++ b/.output/public/engineering/_payload.json
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BsFbFp circuits",{"type":12,"tag":42,"props":74,"children":75},{},[76],{"type":18,"value":77},"​\tGenetic circuit: Gene recA and BsFbFp were all synthesized by Sangon Biotech according to BIOBRICK standard, and the gene circuit was obtained by PCR and verified by agarose gel electrophoresis.",{"type":12,"tag":42,"props":79,"children":80},{},[81],{"type":12,"tag":57,"props":82,"children":85},{"alt":83,"src":84},"build results of BsFbFp ","https://static.igem.wiki/teams/5358/engineering-success/build-results-of-bsfbfp.png",[],{"type":12,"tag":42,"props":87,"children":88},{},[89],{"type":12,"tag":66,"props":90,"children":92},{"className":91},[69],[93],{"type":18,"value":94},"Figure 2. Build results of BsFbfP",{"type":12,"tag":42,"props":96,"children":97},{},[98],{"type":18,"value":99},"​Construction of plasmid: The results of the agarose gel electrophoresis successfully showed bands for recA, the vector and BsFbFp, confirming that the gene circuit was successfully connected to the vector.",{"type":12,"tag":42,"props":101,"children":102},{},[103],{"type":18,"value":104},"​Ideal Strain: The successfully constructed plasmid was transformed into BL21 competent cells and cultured on LB solid medium with ampicillin.",{"type":12,"tag":35,"props":106,"children":108},{"id":107},"test",[109],{"type":18,"value":110},"Test",{"type":12,"tag":42,"props":112,"children":113},{},[114],{"type":18,"value":115},"​Adding 600μL of bacterial solution and 30μL of ampicillin to 30mL of fresh culture medium, logarithmic growth was performed. Then, NA (Nalidixic Acid) was added in a gradient based on concentration to induce the bacteria. Subsequently, 250μL from each group was added to a anaerobic chamber and incubated on a shaking incubator at 37°C with a speed of 180 r/min. After 2 hours, the OD value and fluorescence intensity were measured.",{"type":12,"tag":42,"props":117,"children":118},{},[119],{"type":12,"tag":57,"props":120,"children":123},{"alt":121,"src":122},"RFU-BsFbFp in 10lianpaichip(anaerobic chamber)","https://static.igem.wiki/teams/5358/engineering-success/rfu-bsfbfp-in-10lianpaichip-anaerobic-chamber.jpg",[],{"type":12,"tag":42,"props":125,"children":126},{},[127],{"type":12,"tag":66,"props":128,"children":130},{"className":129},[69],[131],{"type":18,"value":132},"Figure 3. RFU-BsFbFp in 10lianpaichip(anaerobic chamber)",{"type":12,"tag":42,"props":134,"children":135},{},[136],{"type":18,"value":137},"​The RFU (Relative Fluorescence Units) of the three groups of engineered bacteria increased linearly with the amount of NA (Nalidixic Acid) added. The results indicate that the fluorescent expression circuit of BsFbFp has been successfully constructed, and the engineered bacteria can effectively express fluorescence in an anaerobic environment.",{"type":12,"tag":35,"props":139,"children":141},{"id":140},"learn",[142],{"type":18,"value":143},"Learn",{"type":12,"tag":42,"props":145,"children":146},{},[147],{"type":18,"value":148},"​In conclusion，the plasmid constructed using BsFbFp as a reporter gene can effectively express fluorescence in low-oxygen or anaerobic environments, which is beneficial for the design of the hardware components.",{"type":12,"tag":42,"props":150,"children":151},{},[152],{"type":18,"value":153},"​So we believed that the work in this cycle has been successfully accomplished., and we obtained the engineered bacteria needed for our goals.",{"type":12,"tag":21,"props":155,"children":157},{"id":156},"and-gate-design",[158],{"type":18,"value":159},"AND Gate Design",{"type":12,"tag":28,"props":161,"children":163},{"id":162},"circle-1-design-pet28-reca-hrp-egfp",[164],{"type":18,"value":165},"Circle 1: Design pET28-recA-HRP-egfp",{"type":12,"tag":35,"props":167,"children":169},{"id":168},"design-1",[170],{"type":18,"value":40},{"type":12,"tag":42,"props":172,"children":173},{},[174],{"type":18,"value":175},"​To construct the and gate, we introduced the hrp system, composed of the hrpR gene and hrpS gene, as well as the HrpL promoter regulated by the sigma54 factor. The hrpL promoter will only be activated when both the hrpR gene and the hrpS gene are expressed simultaneously.",{"type":12,"tag":42,"props":177,"children":178},{},[179],{"type":18,"value":180},"​In 2023, the BIT team successfully constructed P recA, the Hrp system, and egfp on the pUC19 plasmid. However, tests showed that the expression level of the circuit constructed using the pUC19 plasmid as a vector was relatively low, significantly deviating from the expected results. Therefore, we plan to switch to the pET28 plasmid, which has a more prominent expression level, to facilitate the synergistic assessment of endocrine disruption effects and oxidative damage effects.",{"type":12,"tag":35,"props":182,"children":184},{"id":183},"build-1",[185],{"type":18,"value":52},{"type":12,"tag":42,"props":187,"children":188},{},[189],{"type":18,"value":190},"​Genetic circuit: recA-HRP-egfp were synthesized by Sangon Biotech according to BIOBRICK standard， the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR.",{"type":12,"tag":42,"props":192,"children":193},{},[194],{"type":12,"tag":57,"props":195,"children":198},{"alt":196,"src":197},"Cir1-1","https://static.igem.wiki/teams/5358/engineering-success/cir1-1.png",[],{"type":12,"tag":42,"props":200,"children":201},{},[202],{"type":12,"tag":66,"props":203,"children":205},{"className":204},[69],[206],{"type":18,"value":207},"Figure 4. enzyme digestion result of circle 1",{"type":12,"tag":42,"props":209,"children":210},{},[211],{"type":18,"value":212},"​\nThe results of the agarose gel electrophoresis indicated that the vector was successfully digested and the target fragment was successfully amplified by PCR. The ligation was carried out at a 1:4 ratio of vector to target fragment, followed by transformation into DH5α competent cells and verification through agarose gel electrophoresis.",{"type":12,"tag":42,"props":214,"children":215},{},[216],{"type":12,"tag":57,"props":217,"children":220},{"alt":218,"src":219},"Cir1-2","https://static.igem.wiki/teams/5358/engineering-success/cir1-2.png",[],{"type":12,"tag":42,"props":222,"children":223},{},[224],{"type":12,"tag":66,"props":225,"children":227},{"className":226},[69],[228],{"type":18,"value":229},"Figure 5. build result of circle 1",{"type":12,"tag":42,"props":231,"children":232},{},[233],{"type":18,"value":234},"​\nVerification results: In multiple rounds of construction validation experiments, the target fragment showed no band in the agarose gel electrophoresis results, and the restriction enzyme digestion verification indicated self-ligation of the vector, proving that the construction in this cycle has failed.",{"type":12,"tag":35,"props":236,"children":238},{"id":237},"test-1",[239],{"type":18,"value":110},{"type":12,"tag":42,"props":241,"children":242},{},[243],{"type":18,"value":244},"​Due to the failure of the target plasmid construction in this cycle, it is necessary to analyze the issues present in the plasmid design and construction process and make corrections.",{"type":12,"tag":35,"props":246,"children":248},{"id":247},"learn-1",[249],{"type":18,"value":143},{"type":12,"tag":42,"props":251,"children":252},{},[253],{"type":18,"value":254},"​During this construction process, we overlooked the reagent concentration ratio of the vector to the target fragment in our ligation system, which may be a major factor contributing to the failure of ligation. We plan to introduce the concentration factor of the ligation reagents into the ratio to improve the efficiency of ligation.",{"type":12,"tag":28,"props":256,"children":258},{"id":257},"circle-2-adjust-the-ligation-system-and-reconstruct-the-plasmid-pet28-reca-hrp-egfp",[259],{"type":18,"value":260},"Circle 2: Adjust the ligation system and reconstruct the plasmid pET28-recA-HRP-egfp.",{"type":12,"tag":35,"props":262,"children":264},{"id":263},"design-2",[265],{"type":18,"value":40},{"type":12,"tag":42,"props":267,"children":268},{},[269],{"type":18,"value":270},"​Based on the experience learned from Circle 1, we plan to use a new ligation system, increase the dosage of the target fragment, and improve the ligation efficiency.",{"type":12,"tag":35,"props":272,"children":274},{"id":273},"build-2",[275],{"type":18,"value":52},{"type":12,"tag":42,"props":277,"children":278},{},[279],{"type":18,"value":280},"​The construction process used three different ligation systems, with the ratios of vector to target fragment being 1:4, 1:5, and 1:6, respectively. The constructed plasmids were extracted and verified by PCR followed by agarose gel electrophoresis.",{"type":12,"tag":42,"props":282,"children":283},{},[284],{"type":12,"tag":57,"props":285,"children":288},{"alt":286,"src":287},"Cir2","https://static.igem.wiki/teams/5358/engineering-success/cir2.png",[],{"type":12,"tag":42,"props":290,"children":291},{},[292],{"type":12,"tag":66,"props":293,"children":295},{"className":294},[69],[296],{"type":18,"value":297},"Figure 6. built result of circle 2",{"type":12,"tag":42,"props":299,"children":300},{},[301],{"type":18,"value":302},"​\nConstruction of plasmid: In the multi-round adjustment experiments of the ligation system construction, the agarose gel electrophoresis results still did not show a band for the target fragment, indicating that the construction in this cycle was unsuccessful.",{"type":12,"tag":35,"props":304,"children":306},{"id":305},"test-2",[307],{"type":18,"value":110},{"type":12,"tag":42,"props":309,"children":310},{},[311],{"type":18,"value":312},"​Due to the failure of target plasmid construction in this cycle, testing cannot be carried out.",{"type":12,"tag":35,"props":314,"children":316},{"id":315},"learn-2",[317],{"type":18,"value":143},{"type":12,"tag":42,"props":319,"children":320},{},[321],{"type":18,"value":322},"​In this cycle, we have corrected the ligation system, removing this influencing factor. Therefore, we suspect that the low partial digestion efficiency at the target sequence enzyme cutting sites may be the cause. This could be due to issues with the setting of protective bases near the enzyme cutting sites when designing the PCR primers, leading to another ligation failure. As such, we plan to redesign the PCR primers and increase the number of protective bases on both sides of the enzyme cutting sites from one or none to three to improve the success rate of the ligation.",{"type":12,"tag":28,"props":324,"children":326},{"id":325},"circle-3-redesign-the-primers-increase-protective-bases-and-reconstruct-pet28-reca-hrp-egfp",[327],{"type":18,"value":328},"Circle 3: Redesign the primers, increase protective bases, and reconstruct pET28-recA-HRP-egfp",{"type":12,"tag":35,"props":330,"children":332},{"id":331},"design-3",[333],{"type":18,"value":40},{"type":12,"tag":42,"props":335,"children":336},{},[337],{"type":18,"value":338},"​Based on the experience learned from Circle 2, we have redesigned the PCR primers for the target fragment and added protective bases to them, and we are reconstructing the pET28-recA-HRP-egfp plasmid.",{"type":12,"tag":35,"props":340,"children":342},{"id":341},"build-3",[343],{"type":18,"value":52},{"type":12,"tag":42,"props":345,"children":346},{},[347],{"type":18,"value":348},"​After changing to new PCR primers and setting the protective bases on both sides of the restriction enzyme cutting sites to 3, the construction was carried out. Genetic circuits: recA-HRP-egfp were synthesized by Sangon Biotech according the BIOBRICK standard, the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR.",{"type":12,"tag":42,"props":350,"children":351},{},[352],{"type":12,"tag":57,"props":353,"children":356},{"alt":354,"src":355},"Cir3","https://static.igem.wiki/teams/5358/engineering-success/cir3.png",[],{"type":12,"tag":42,"props":358,"children":359},{},[360],{"type":12,"tag":66,"props":361,"children":363},{"className":362},[69],[364],{"type":18,"value":365},"Figure 7. built result of circle 3",{"type":12,"tag":42,"props":367,"children":368},{},[369],{"type":18,"value":370},"​\nThe agarose gel electrophoresis results showed that after redesigning the PCR primers and increasing the number of protective bases on both sides of the restriction enzyme cutting sites from one or none to three, only one band was observed for the target fragment, indicating that the new PCR primers have strong specificity and the target fragment was successfully obtained.\n​\tHowever, due to time constraints, we did not proceed with the subsequent experiments.",{"type":12,"tag":35,"props":372,"children":374},{"id":373},"test-3",[375],{"type":18,"value":110},{"type":12,"tag":42,"props":377,"children":378},{},[379],{"type":18,"value":380},"​Limited by time, we were unable to complete the reconstruction, and therefore have no test results.",{"type":12,"tag":35,"props":382,"children":384},{"id":383},"learn-3",[385],{"type":18,"value":143},{"type":12,"tag":42,"props":387,"children":388},{},[389],{"type":18,"value":390},"​In this cycle, the new PCR primers and the addition of protective bases at both ends of the restriction enzyme sites are beneficial for improving the success rate of enzyme ligation, thereby providing an experimental basis for the successful construction of the gate circuit.",{"type":12,"tag":13,"props":392,"children":394},{"id":393},"hardware",[395],{"type":18,"value":396},"Hardware",{"type":12,"tag":28,"props":398,"children":400},{"id":399},"objectives",[401],{"type":18,"value":402},"Objectives",{"type":12,"tag":404,"props":405,"children":406},"ol",{},[407,413,418],{"type":12,"tag":408,"props":409,"children":410},"li",{},[411],{"type":18,"value":412},"To provide a high-throughput portable reaction vessel.",{"type":12,"tag":408,"props":414,"children":415},{},[416],{"type":18,"value":417},"To provide stable physical conditions for detection.",{"type":12,"tag":408,"props":419,"children":420},{},[421],{"type":18,"value":422},"High-sensitivity fluorescence detection.",{"type":12,"tag":28,"props":424,"children":426},{"id":425},"design-4",[427],{"type":18,"value":40},{"type":12,"tag":42,"props":429,"children":430},{},[431],{"type":18,"value":432},"​Our entire device mainly consists of three major parts: the outer casing that encases the entire device, the reaction area located in the upper layer, and the detection area situated in the lower layer. In the initial design, we utilized an STM32 chip as the main controller to regulate temperature control, fluorescence detection, and Bluetooth communication functions. In the second iteration, we replaced the main controller with an ESP32 chip that integrates a wireless communication module, and we adopted integrated temperature control and detection modules, which further enhances the system's stability and accuracy. To meet the need for simultaneous detection of multiple samples, we also redesigned the high-throughput reaction chambers, achieving rapid successive detection through a linear slide rail system.",{"type":12,"tag":434,"props":435,"children":436},"table",{},[437,484],{"type":12,"tag":438,"props":439,"children":440},"thead",{},[441],{"type":12,"tag":442,"props":443,"children":444},"tr",{},[445,449,462,473],{"type":12,"tag":446,"props":447,"children":448},"th",{},[],{"type":12,"tag":446,"props":450,"children":451},{},[452],{"type":12,"tag":453,"props":454,"children":455},"em",{},[456],{"type":12,"tag":457,"props":458,"children":459},"strong",{},[460],{"type":18,"value":461},"Initial Scheme",{"type":12,"tag":446,"props":463,"children":464},{},[465],{"type":12,"tag":453,"props":466,"children":467},{},[468],{"type":12,"tag":457,"props":469,"children":470},{},[471],{"type":18,"value":472},"Final Scheme",{"type":12,"tag":446,"props":474,"children":475},{},[476],{"type":12,"tag":453,"props":477,"children":478},{},[479],{"type":12,"tag":457,"props":480,"children":481},{},[482],{"type":18,"value":483},"Advantages After Improvement",{"type":12,"tag":485,"props":486,"children":487},"tbody",{},[488,518,547,576],{"type":12,"tag":442,"props":489,"children":490},{},[491,503,508,513],{"type":12,"tag":492,"props":493,"children":494},"td",{},[495],{"type":12,"tag":453,"props":496,"children":497},{},[498],{"type":12,"tag":457,"props":499,"children":500},{},[501],{"type":18,"value":502},"MCU",{"type":12,"tag":492,"props":504,"children":505},{},[506],{"type":18,"value":507},"STM32F103C8T6",{"type":12,"tag":492,"props":509,"children":510},{},[511],{"type":18,"value":512},"Arduino Nano ESP32",{"type":12,"tag":492,"props":514,"children":515},{},[516],{"type":18,"value":517},"Integrate Bluetooth communication module, reduce the complexity of the design",{"type":12,"tag":442,"props":519,"children":520},{},[521,532,537,542],{"type":12,"tag":492,"props":522,"children":523},{},[524],{"type":12,"tag":453,"props":525,"children":526},{},[527],{"type":12,"tag":457,"props":528,"children":529},{},[530],{"type":18,"value":531},"Temperature Control",{"type":12,"tag":492,"props":533,"children":534},{},[535],{"type":18,"value":536},"TB6612FNG",{"type":12,"tag":492,"props":538,"children":539},{},[540],{"type":18,"value":541},"TCM1040",{"type":12,"tag":492,"props":543,"children":544},{},[545],{"type":18,"value":546},"More precise control of temperature",{"type":12,"tag":442,"props":548,"children":549},{},[550,561,566,571],{"type":12,"tag":492,"props":551,"children":552},{},[553],{"type":12,"tag":453,"props":554,"children":555},{},[556],{"type":12,"tag":457,"props":557,"children":558},{},[559],{"type":18,"value":560},"Fluorescence Detection",{"type":12,"tag":492,"props":562,"children":563},{},[564],{"type":18,"value":565},"AS7341",{"type":12,"tag":492,"props":567,"children":568},{},[569],{"type":18,"value":570},"FM-4A",{"type":12,"tag":492,"props":572,"children":573},{},[574],{"type":18,"value":575},"Integrate excitation light and fluorescent sensor to make the detection result more stable.",{"type":12,"tag":442,"props":577,"children":578},{},[579,590,595,600],{"type":12,"tag":492,"props":580,"children":581},{},[582],{"type":12,"tag":453,"props":583,"children":584},{},[585],{"type":12,"tag":457,"props":586,"children":587},{},[588],{"type":18,"value":589},"Reaction Platform",{"type":12,"tag":492,"props":591,"children":592},{},[593],{"type":18,"value":594},"Single Reaction Tube",{"type":12,"tag":492,"props":596,"children":597},{},[598],{"type":18,"value":599},"Slide Rail Platform",{"type":12,"tag":492,"props":601,"children":602},{},[603],{"type":18,"value":604},"Achieve high-throughput detection",{"type":12,"tag":42,"props":606,"children":607},{},[608],{"type":12,"tag":66,"props":609,"children":612},{"className":610},[611],"tab-title",[613],{"type":18,"value":614},"Table 1. Scheme difference",{"type":12,"tag":42,"props":616,"children":617},{},[618],{"type":18,"value":619},"​\nIn terms of material selection, we have adopted a black silicone kit and an aluminum alloy temperature-controlled slot as the environment for the centrifuge tubes. This scheme can excellently meet the insulation requirements of the design.",{"type":12,"tag":42,"props":621,"children":622},{},[623],{"type":12,"tag":57,"props":624,"children":627},{"alt":625,"src":626},"Edition Scheme Process","https://static.igem.wiki/teams/5358/engineering-success/edition-scheme-process.png",[],{"type":12,"tag":42,"props":629,"children":630},{},[631],{"type":12,"tag":66,"props":632,"children":634},{"className":633},[69],[635],{"type":18,"value":636},"Figure 8. Edition Scheme Process",{"type":12,"tag":28,"props":638,"children":640},{"id":639},"establishment",[641],{"type":18,"value":642},"Establishment",{"type":12,"tag":42,"props":644,"children":645},{},[646],{"type":18,"value":647},"​\tThe system construction utilized common integrated modules, with the main controller being an ESP32 chip, and temperature control and detection were accomplished using the TCM temperature control module and LIDE Technology's FM series digital fluorescence detection module, respectively. We employed industrial processing techniques to fabricate the aluminum alloy temperature-controlled slots, while the casing and other components were created using 3D printing technology.",{"type":12,"tag":42,"props":649,"children":650},{},[651],{"type":12,"tag":57,"props":652,"children":655},{"alt":653,"src":654},"Schematic Diagram of Hardware Equipment","https://static.igem.wiki/teams/5358/engineering-success/new.png",[],{"type":12,"tag":42,"props":657,"children":658},{},[659],{"type":12,"tag":66,"props":660,"children":662},{"className":661},[69],[663],{"type":18,"value":664},"Figure 9. Schematic Diagram of Hardware Equipment",{"type":12,"tag":42,"props":666,"children":667},{},[668],{"type":12,"tag":57,"props":669,"children":672},{"alt":670,"src":671},"Model Diagram of Hardware Equipment","https://static.igem.wiki/teams/5358/engineering-success/model-diagram-of-hardware-equipment.png",[],{"type":12,"tag":42,"props":674,"children":675},{},[676],{"type":12,"tag":66,"props":677,"children":679},{"className":678},[69],[680],{"type":18,"value":681},"Figure 10. Model Diagram of Hardware Equipment",{"type":12,"tag":28,"props":683,"children":685},{"id":684},"test-4",[686],{"type":18,"value":110},{"type":12,"tag":42,"props":688,"children":689},{},[690],{"type":18,"value":691},"​\tAfter finalizing the initial plan, we first carried out feasibility tests to verify the functionality of the fluorescence system and the temperature control system. After assembling the initial version of the model, numerous issues were identified through usage testing and preliminary risk analysis, such as the susceptibility of the fluorescence system to interference and the instability of the temperature control system. Through a cycle of testing, feedback, improvement, and retesting, the final version of the model demonstrated excellent performance across all aspects. Experiments showed that fluorescence identification was highly accurate, and the overall aesthetics were significantly enhanced.",{"type":12,"tag":42,"props":693,"children":694},{},[695],{"type":12,"tag":57,"props":696,"children":699},{"alt":697,"src":698},"Results of Temperature Control Experiment","https://static.igem.wiki/teams/5358/engineering-success/results-of-temperature-control-experiment.png",[],{"type":12,"tag":42,"props":701,"children":702},{},[703],{"type":12,"tag":66,"props":704,"children":706},{"className":705},[69],[707],{"type":18,"value":708},"Figure 11. Results of Temperature Control Experiment",{"type":12,"tag":42,"props":710,"children":711},{},[712],{"type":12,"tag":57,"props":713,"children":716},{"alt":714,"src":715},"Results of Flurescence Verification Experiment","https://static.igem.wiki/teams/5358/engineering-success/results-of-flurescence-verification-experiment.png",[],{"type":12,"tag":42,"props":718,"children":719},{},[720],{"type":12,"tag":66,"props":721,"children":723},{"className":722},[69],[724],{"type":18,"value":725},"Figure 12. Results of Flurescence Verification Experiment",{"type":12,"tag":28,"props":727,"children":729},{"id":728},"learn-4",[730],{"type":18,"value":143},{"type":12,"tag":42,"props":732,"children":733},{},[734],{"type":18,"value":735},"​\tDuring the process of designing the device, we encountered various challenges, such as overly complex control systems, insufficient stability, inability to conduct multi-sample testing, and issues with the optical path design. Throughout the real-time testing and discussion phases, we continuously documented various issues, proposed improvement plans, and actively sought help from knowledgeable teachers and consultants. Eventually, we completed a hardware design that met the requirements. Through continuous learning, improvement, and questioning, our expertise in the relevant fields has been greatly boosted.",{"type":12,"tag":13,"props":737,"children":739},{"id":738},"software",[740],{"type":18,"value":741},"Software",{"type":12,"tag":28,"props":743,"children":745},{"id":744},"objectives-1",[746],{"type":18,"value":402},{"type":12,"tag":404,"props":748,"children":749},{},[750,755,760],{"type":12,"tag":408,"props":751,"children":752},{},[753],{"type":18,"value":754},"To implement human-machine interaction for information exchange with hardware.",{"type":12,"tag":408,"props":756,"children":757},{},[758],{"type":18,"value":759},"To automatically process data sent by the hardware.",{"type":12,"tag":408,"props":761,"children":762},{},[763],{"type":18,"value":764},"To visualize data.",{"type":12,"tag":28,"props":766,"children":768},{"id":767},"design-5",[769],{"type":18,"value":40},{"type":12,"tag":42,"props":771,"children":772},{},[773],{"type":18,"value":774},"​The main body is divided into two parts—the Bluetooth reception module and the plotting module.",{"type":12,"tag":28,"props":776,"children":778},{"id":777},"bluetooth-module-receives-data",[779],{"type":18,"value":780},"Bluetooth module receives data",{"type":12,"tag":782,"props":783,"children":785},"pre",{"code":784},"CREATE discoveryAgent AS new BluetoothDeviceDiscoveryAgent\n​\nCONNECT discoveryAgent.deviceDiscovered TO this.deviceDiscovered\nCONNECT discoveryAgent.finished TO this.onDeviceConnected\n​\n// Start searching for Bluetooth devices\nCALL discoveryAgent.start()\n​\n// Initialize Bluetooth Socket\nCREATE socket AS new BluetoothSocket USING RfcommProtocol\n​\n// Connect button click signal to send STARTSTART command\nCONNECT ui.pushButton.clicked TO this.sendStartSignal\n",[786],{"type":12,"tag":787,"props":788,"children":789},"code",{"__ignoreMap":5},[790],{"type":18,"value":784},{"type":12,"tag":28,"props":792,"children":794},{"id":793},"plotting-module-performs-data-visualization",[795],{"type":18,"value":796},"Plotting module performs data visualization",{"type":12,"tag":782,"props":798,"children":800},{"code":799},"FUNCTION updateGraphs()\n  // Create canvas\n  CREATE new scene\n  SET scene to graphicsView\n​\n  // Draw temperature graph\n  FOR i FROM 1 TO size of temperatureData - 1 DO\n    ADD line FROM (i-1, temperatureData[i-1]) TO (i, temperatureData[i]) WITH color red\n​\n  // Draw fluorescence intensity graph\n  FOR i FROM 1 TO size of fluorescenceData - 1 DO\n    ADD line FROM (i-1, fluorescenceData[i-1]) TO (i, fluorescenceData[i]) WITH color blue\n​\n  UPDATE graphicsView\nEND FUNCTION\n",[801],{"type":12,"tag":787,"props":802,"children":803},{"__ignoreMap":5},[804],{"type":18,"value":799},{"type":12,"tag":28,"props":806,"children":808},{"id":807},"data-segmentation-module",[809],{"type":18,"value":810},"Data segmentation module",{"type":12,"tag":782,"props":812,"children":814},{"code":813},"FUNCTION splitAndFindMax(dataPoints, zeroThresholdCount)\n    INITIALIZE maxValues AS empty list\n    INITIALIZE currentSegment AS empty list\n    SET zeroCount TO 0\n​\n    FOR each point IN dataPoints DO\n        IF point.value == 0.0 THEN\n            INCREMENT zeroCount\n            IF zeroCount >= zeroThresholdCount THEN\n                IF currentSegment is not empty THEN\n                    SET maxVal TO maximum value in currentSegment\n                    ADD maxVal TO maxValues\n                    CLEAR currentSegment\n                    SET zeroCount TO 0  // Reset the counter​\n                CONTINUE  // Skip the current point, do not include in currentSegment​\n            END IF\n        ELSE\n            SET zeroCount TO 0  // Reset the counter because a non-zero value was encountered\n        END IF\n​\n        //Add the current point to the currentSegment\n        ADD point TO currentSegment\n    END FOR\n​\n    // Process the last segment\n    IF currentSegment is not empty THEN\n        SET maxVal TO maximum value in currentSegment\n        ADD maxVal TO maxValues\n    END IF\n    RETURN maxValues\nEND FUNCTION\n",[815],{"type":12,"tag":787,"props":816,"children":817},{"__ignoreMap":5},[818],{"type":18,"value":813},{"type":12,"tag":28,"props":820,"children":822},{"id":821},"testing",[823],{"type":18,"value":824},"Testing",{"title":5,"searchDepth":826,"depth":827,"links":828},1,3,[829,841,848],{"id":15,"depth":830,"text":19,"children":831},2,[832,836],{"id":23,"depth":827,"text":26,"children":833},[834],{"id":30,"depth":835,"text":33},4,{"id":156,"depth":827,"text":159,"children":837},[838,839,840],{"id":162,"depth":835,"text":165},{"id":257,"depth":835,"text":260},{"id":325,"depth":835,"text":328},{"id":393,"depth":830,"text":396,"children":842},[843,844,845,846,847],{"id":399,"depth":835,"text":402},{"id":425,"depth":835,"text":40},{"id":639,"depth":835,"text":642},{"id":684,"depth":835,"text":110},{"id":728,"depth":835,"text":143},{"id":738,"depth":830,"text":741,"children":849},[850,851,852,853,854,855],{"id":744,"depth":835,"text":402},{"id":767,"depth":835,"text":40},{"id":777,"depth":835,"text":780},{"id":793,"depth":835,"text":796},{"id":807,"depth":835,"text":810},{"id":821,"depth":835,"text":824},"markdown","content:project-engineering.md","content","project-engineering.md","project-engineering","md",1727868995259]
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+[{"data":1,"prerenderedAt":862},["Reactive",2],{"content-query-UXJZW5OGcq":3},{"_path":4,"_dir":5,"_draft":6,"_partial":6,"_locale":5,"title":7,"description":5,"body":8,"_type":856,"_id":857,"_source":858,"_file":859,"_stem":860,"_extension":861},"/project-engineering","",false,"Project Engineering",{"type":9,"children":10,"toc":825},"root",[11,20,27,34,41,47,53,62,73,78,86,95,100,105,111,116,124,133,138,144,149,154,160,166,171,176,181,186,191,199,208,213,221,230,235,240,245,250,255,261,266,271,276,281,289,298,303,308,313,318,323,329,334,339,344,349,357,366,371,376,381,386,391,397,403,423,428,433,605,615,620,628,637,643,648,656,665,673,682,687,692,700,709,717,726,731,736,742,747,765,770,775,781,791,797,805,811,819],{"type":12,"tag":13,"props":14,"children":16},"element","h2",{"id":15},"biosensor",[17],{"type":18,"value":19},"text","Biosensor",{"type":12,"tag":21,"props":22,"children":24},"h3",{"id":23},"luminescence",[25],{"type":18,"value":26},"Luminescence",{"type":12,"tag":28,"props":29,"children":31},"h4",{"id":30},"circle-design-reca-bsfbfp",[32],{"type":18,"value":33},"Circle: Design recA-BsFbFp",{"type":12,"tag":35,"props":36,"children":38},"h5",{"id":37},"design",[39],{"type":18,"value":40},"Design",{"type":12,"tag":42,"props":43,"children":44},"p",{},[45],{"type":18,"value":46},"​In order to adapt to the low oxygen environment of the hardware design, we replaced the egfp gene, which strictly requires oxygen molecules as a cofactor for its luminescence, with the reporter gene BsFbFp. This gene can effectively express fluorescent signals in low or anaerobic environments.",{"type":12,"tag":35,"props":48,"children":50},{"id":49},"build",[51],{"type":18,"value":52},"Build",{"type":12,"tag":42,"props":54,"children":55},{},[56],{"type":12,"tag":57,"props":58,"children":61},"img",{"alt":59,"src":60},"BsFbFp circuits","https://static.igem.wiki/teams/5358/engineering-success/bsfbfp-circuits.png",[],{"type":12,"tag":42,"props":63,"children":64},{},[65],{"type":12,"tag":66,"props":67,"children":70},"span",{"className":68},[69],"fig-title",[71],{"type":18,"value":72},"Figure 1. BsFbFp circuits",{"type":12,"tag":42,"props":74,"children":75},{},[76],{"type":18,"value":77},"​Genetic circuit: Gene recA and BsFbFp were all synthesized by Sangon Biotech according to BIOBRICK standard, and the gene circuit was obtained by PCR and verified by agarose gel electrophoresis.",{"type":12,"tag":42,"props":79,"children":80},{},[81],{"type":12,"tag":57,"props":82,"children":85},{"alt":83,"src":84},"build results of BsFbFp ","https://static.igem.wiki/teams/5358/engineering-success/build-results-of-bsfbfp.png",[],{"type":12,"tag":42,"props":87,"children":88},{},[89],{"type":12,"tag":66,"props":90,"children":92},{"className":91},[69],[93],{"type":18,"value":94},"Figure 2. Build results of BsFbfP",{"type":12,"tag":42,"props":96,"children":97},{},[98],{"type":18,"value":99},"​Construction of plasmid: The results of the agarose gel electrophoresis successfully showed bands for recA, the vector and BsFbFp, confirming that the gene circuit was successfully connected to the vector.",{"type":12,"tag":42,"props":101,"children":102},{},[103],{"type":18,"value":104},"​Ideal Strain: The successfully constructed plasmid was transformed into BL21 competent cells and cultured on LB solid medium with ampicillin.",{"type":12,"tag":35,"props":106,"children":108},{"id":107},"test",[109],{"type":18,"value":110},"Test",{"type":12,"tag":42,"props":112,"children":113},{},[114],{"type":18,"value":115},"​Adding 600μL of bacterial solution and 30μL of ampicillin to 30mL of fresh culture medium, logarithmic growth was performed. Then, NA (Nalidixic Acid) was added in a gradient based on concentration to induce the bacteria. Subsequently, 250μL from each group was added to a anaerobic chamber and incubated on a shaking incubator at 37°C with a speed of 180 r/min. After 2 hours, the OD value and fluorescence intensity were measured.",{"type":12,"tag":42,"props":117,"children":118},{},[119],{"type":12,"tag":57,"props":120,"children":123},{"alt":121,"src":122},"RFU-BsFbFp in 10lianpaichip(anaerobic chamber)","https://static.igem.wiki/teams/5358/engineering-success/rfu-bsfbfp-in-10lianpaichip-anaerobic-chamber.jpg",[],{"type":12,"tag":42,"props":125,"children":126},{},[127],{"type":12,"tag":66,"props":128,"children":130},{"className":129},[69],[131],{"type":18,"value":132},"Figure 3. RFU-BsFbFp in 10lianpaichip(anaerobic chamber)",{"type":12,"tag":42,"props":134,"children":135},{},[136],{"type":18,"value":137},"​The RFU (Relative Fluorescence Units) of the three groups of engineered bacteria increased linearly with the amount of NA (Nalidixic Acid) added. The results indicate that the fluorescent expression circuit of BsFbFp has been successfully constructed, and the engineered bacteria can effectively express fluorescence in an anaerobic environment.",{"type":12,"tag":35,"props":139,"children":141},{"id":140},"learn",[142],{"type":18,"value":143},"Learn",{"type":12,"tag":42,"props":145,"children":146},{},[147],{"type":18,"value":148},"​In conclusion，the plasmid constructed using BsFbFp as a reporter gene can effectively express fluorescence in low-oxygen or anaerobic environments, which is beneficial for the design of the hardware components.",{"type":12,"tag":42,"props":150,"children":151},{},[152],{"type":18,"value":153},"​So we believed that the work in this cycle has been successfully accomplished., and we obtained the engineered bacteria needed for our goals.",{"type":12,"tag":21,"props":155,"children":157},{"id":156},"and-gate-design",[158],{"type":18,"value":159},"AND Gate Design",{"type":12,"tag":28,"props":161,"children":163},{"id":162},"circle-1-design-pet28-reca-hrp-egfp",[164],{"type":18,"value":165},"Circle 1: Design pET28-recA-HRP-egfp",{"type":12,"tag":35,"props":167,"children":169},{"id":168},"design-1",[170],{"type":18,"value":40},{"type":12,"tag":42,"props":172,"children":173},{},[174],{"type":18,"value":175},"​To construct the and gate, we introduced the hrp system, composed of the hrpR gene and hrpS gene, as well as the HrpL promoter regulated by the sigma54 factor. The hrpL promoter will only be activated when both the hrpR gene and the hrpS gene are expressed simultaneously.",{"type":12,"tag":42,"props":177,"children":178},{},[179],{"type":18,"value":180},"​In 2023, the BIT team successfully constructed P recA, the Hrp system, and egfp on the pUC19 plasmid. However, tests showed that the expression level of the circuit constructed using the pUC19 plasmid as a vector was relatively low, significantly deviating from the expected results. Therefore, we plan to switch to the pET28 plasmid, which has a more prominent expression level, to facilitate the synergistic assessment of endocrine disruption effects and oxidative damage effects.",{"type":12,"tag":35,"props":182,"children":184},{"id":183},"build-1",[185],{"type":18,"value":52},{"type":12,"tag":42,"props":187,"children":188},{},[189],{"type":18,"value":190},"​Genetic circuit: recA-HRP-egfp were synthesized by Sangon Biotech according to BIOBRICK standard， the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR.",{"type":12,"tag":42,"props":192,"children":193},{},[194],{"type":12,"tag":57,"props":195,"children":198},{"alt":196,"src":197},"Cir1-1","https://static.igem.wiki/teams/5358/engineering-success/cir1-1.png",[],{"type":12,"tag":42,"props":200,"children":201},{},[202],{"type":12,"tag":66,"props":203,"children":205},{"className":204},[69],[206],{"type":18,"value":207},"Figure 4. enzyme digestion result of circle 1",{"type":12,"tag":42,"props":209,"children":210},{},[211],{"type":18,"value":212},"​\nThe results of the agarose gel electrophoresis indicated that the vector was successfully digested and the target fragment was successfully amplified by PCR. The ligation was carried out at a 1:4 ratio of vector to target fragment, followed by transformation into DH5α competent cells and verification through agarose gel electrophoresis.",{"type":12,"tag":42,"props":214,"children":215},{},[216],{"type":12,"tag":57,"props":217,"children":220},{"alt":218,"src":219},"Cir1-2","https://static.igem.wiki/teams/5358/engineering-success/cir1-2.png",[],{"type":12,"tag":42,"props":222,"children":223},{},[224],{"type":12,"tag":66,"props":225,"children":227},{"className":226},[69],[228],{"type":18,"value":229},"Figure 5. build result of circle 1",{"type":12,"tag":42,"props":231,"children":232},{},[233],{"type":18,"value":234},"​\nVerification results: In multiple rounds of construction validation experiments, the target fragment showed no band in the agarose gel electrophoresis results, and the restriction enzyme digestion verification indicated self-ligation of the vector, proving that the construction in this cycle has failed.",{"type":12,"tag":35,"props":236,"children":238},{"id":237},"test-1",[239],{"type":18,"value":110},{"type":12,"tag":42,"props":241,"children":242},{},[243],{"type":18,"value":244},"​Due to the failure of the target plasmid construction in this cycle, it is necessary to analyze the issues present in the plasmid design and construction process and make corrections.",{"type":12,"tag":35,"props":246,"children":248},{"id":247},"learn-1",[249],{"type":18,"value":143},{"type":12,"tag":42,"props":251,"children":252},{},[253],{"type":18,"value":254},"​During this construction process, we overlooked the reagent concentration ratio of the vector to the target fragment in our ligation system, which may be a major factor contributing to the failure of ligation. We plan to introduce the concentration factor of the ligation reagents into the ratio to improve the efficiency of ligation.",{"type":12,"tag":28,"props":256,"children":258},{"id":257},"circle-2-adjust-the-ligation-system-and-reconstruct-the-plasmid-pet28-reca-hrp-egfp",[259],{"type":18,"value":260},"Circle 2: Adjust the ligation system and reconstruct the plasmid pET28-recA-HRP-egfp.",{"type":12,"tag":35,"props":262,"children":264},{"id":263},"design-2",[265],{"type":18,"value":40},{"type":12,"tag":42,"props":267,"children":268},{},[269],{"type":18,"value":270},"​Based on the experience learned from Circle 1, we plan to use a new ligation system, increase the dosage of the target fragment, and improve the ligation efficiency.",{"type":12,"tag":35,"props":272,"children":274},{"id":273},"build-2",[275],{"type":18,"value":52},{"type":12,"tag":42,"props":277,"children":278},{},[279],{"type":18,"value":280},"​The construction process used three different ligation systems, with the ratios of vector to target fragment being 1:4, 1:5, and 1:6, respectively. The constructed plasmids were extracted and verified by PCR followed by agarose gel electrophoresis.",{"type":12,"tag":42,"props":282,"children":283},{},[284],{"type":12,"tag":57,"props":285,"children":288},{"alt":286,"src":287},"Cir2","https://static.igem.wiki/teams/5358/engineering-success/cir2.png",[],{"type":12,"tag":42,"props":290,"children":291},{},[292],{"type":12,"tag":66,"props":293,"children":295},{"className":294},[69],[296],{"type":18,"value":297},"Figure 6. built result of circle 2",{"type":12,"tag":42,"props":299,"children":300},{},[301],{"type":18,"value":302},"​\nConstruction of plasmid: In the multi-round adjustment experiments of the ligation system construction, the agarose gel electrophoresis results still did not show a band for the target fragment, indicating that the construction in this cycle was unsuccessful.",{"type":12,"tag":35,"props":304,"children":306},{"id":305},"test-2",[307],{"type":18,"value":110},{"type":12,"tag":42,"props":309,"children":310},{},[311],{"type":18,"value":312},"​Due to the failure of target plasmid construction in this cycle, testing cannot be carried out.",{"type":12,"tag":35,"props":314,"children":316},{"id":315},"learn-2",[317],{"type":18,"value":143},{"type":12,"tag":42,"props":319,"children":320},{},[321],{"type":18,"value":322},"​In this cycle, we have corrected the ligation system, removing this influencing factor. Therefore, we suspect that the low partial digestion efficiency at the target sequence enzyme cutting sites may be the cause. This could be due to issues with the setting of protective bases near the enzyme cutting sites when designing the PCR primers, leading to another ligation failure. As such, we plan to redesign the PCR primers and increase the number of protective bases on both sides of the enzyme cutting sites from one or none to three to improve the success rate of the ligation.",{"type":12,"tag":28,"props":324,"children":326},{"id":325},"circle-3-redesign-the-primers-increase-protective-bases-and-reconstruct-pet28-reca-hrp-egfp",[327],{"type":18,"value":328},"Circle 3: Redesign the primers, increase protective bases, and reconstruct pET28-recA-HRP-egfp",{"type":12,"tag":35,"props":330,"children":332},{"id":331},"design-3",[333],{"type":18,"value":40},{"type":12,"tag":42,"props":335,"children":336},{},[337],{"type":18,"value":338},"​Based on the experience learned from Circle 2, we have redesigned the PCR primers for the target fragment and added protective bases to them, and we are reconstructing the pET28-recA-HRP-egfp plasmid.",{"type":12,"tag":35,"props":340,"children":342},{"id":341},"build-3",[343],{"type":18,"value":52},{"type":12,"tag":42,"props":345,"children":346},{},[347],{"type":18,"value":348},"​After changing to new PCR primers and setting the protective bases on both sides of the restriction enzyme cutting sites to 3, the construction was carried out. Genetic circuits: recA-HRP-egfp were synthesized by Sangon Biotech according the BIOBRICK standard, the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR.",{"type":12,"tag":42,"props":350,"children":351},{},[352],{"type":12,"tag":57,"props":353,"children":356},{"alt":354,"src":355},"Cir3","https://static.igem.wiki/teams/5358/engineering-success/cir3.png",[],{"type":12,"tag":42,"props":358,"children":359},{},[360],{"type":12,"tag":66,"props":361,"children":363},{"className":362},[69],[364],{"type":18,"value":365},"Figure 7. built result of circle 3",{"type":12,"tag":42,"props":367,"children":368},{},[369],{"type":18,"value":370},"​\nThe agarose gel electrophoresis results showed that after redesigning the PCR primers and increasing the number of protective bases on both sides of the restriction enzyme cutting sites from one or none to three, only one band was observed for the target fragment, indicating that the new PCR primers have strong specificity and the target fragment was successfully obtained.\n​\nHowever, due to time constraints, we did not proceed with the subsequent experiments.",{"type":12,"tag":35,"props":372,"children":374},{"id":373},"test-3",[375],{"type":18,"value":110},{"type":12,"tag":42,"props":377,"children":378},{},[379],{"type":18,"value":380},"​Limited by time, we were unable to complete the reconstruction, and therefore have no test results.",{"type":12,"tag":35,"props":382,"children":384},{"id":383},"learn-3",[385],{"type":18,"value":143},{"type":12,"tag":42,"props":387,"children":388},{},[389],{"type":18,"value":390},"​In this cycle, the new PCR primers and the addition of protective bases at both ends of the restriction enzyme sites are beneficial for improving the success rate of enzyme ligation, thereby providing an experimental basis for the successful construction of the gate circuit.",{"type":12,"tag":13,"props":392,"children":394},{"id":393},"hardware",[395],{"type":18,"value":396},"Hardware",{"type":12,"tag":28,"props":398,"children":400},{"id":399},"objectives",[401],{"type":18,"value":402},"Objectives",{"type":12,"tag":404,"props":405,"children":406},"ol",{},[407,413,418],{"type":12,"tag":408,"props":409,"children":410},"li",{},[411],{"type":18,"value":412},"To provide a high-throughput portable reaction vessel.",{"type":12,"tag":408,"props":414,"children":415},{},[416],{"type":18,"value":417},"To provide stable physical conditions for detection.",{"type":12,"tag":408,"props":419,"children":420},{},[421],{"type":18,"value":422},"High-sensitivity fluorescence detection.",{"type":12,"tag":28,"props":424,"children":426},{"id":425},"design-4",[427],{"type":18,"value":40},{"type":12,"tag":42,"props":429,"children":430},{},[431],{"type":18,"value":432},"​Our entire device mainly consists of three major parts: the outer casing that encases the entire device, the reaction area located in the upper layer, and the detection area situated in the lower layer. In the initial design, we utilized an STM32 chip as the main controller to regulate temperature control, fluorescence detection, and Bluetooth communication functions. In the second iteration, we replaced the main controller with an ESP32 chip that integrates a wireless communication module, and we adopted integrated temperature control and detection modules, which further enhances the system's stability and accuracy. To meet the need for simultaneous detection of multiple samples, we also redesigned the high-throughput reaction chambers, achieving rapid successive detection through a linear slide rail system.",{"type":12,"tag":434,"props":435,"children":436},"table",{},[437,484],{"type":12,"tag":438,"props":439,"children":440},"thead",{},[441],{"type":12,"tag":442,"props":443,"children":444},"tr",{},[445,449,462,473],{"type":12,"tag":446,"props":447,"children":448},"th",{},[],{"type":12,"tag":446,"props":450,"children":451},{},[452],{"type":12,"tag":453,"props":454,"children":455},"em",{},[456],{"type":12,"tag":457,"props":458,"children":459},"strong",{},[460],{"type":18,"value":461},"Initial Scheme",{"type":12,"tag":446,"props":463,"children":464},{},[465],{"type":12,"tag":453,"props":466,"children":467},{},[468],{"type":12,"tag":457,"props":469,"children":470},{},[471],{"type":18,"value":472},"Final Scheme",{"type":12,"tag":446,"props":474,"children":475},{},[476],{"type":12,"tag":453,"props":477,"children":478},{},[479],{"type":12,"tag":457,"props":480,"children":481},{},[482],{"type":18,"value":483},"Advantages After Improvement",{"type":12,"tag":485,"props":486,"children":487},"tbody",{},[488,518,547,576],{"type":12,"tag":442,"props":489,"children":490},{},[491,503,508,513],{"type":12,"tag":492,"props":493,"children":494},"td",{},[495],{"type":12,"tag":453,"props":496,"children":497},{},[498],{"type":12,"tag":457,"props":499,"children":500},{},[501],{"type":18,"value":502},"MCU",{"type":12,"tag":492,"props":504,"children":505},{},[506],{"type":18,"value":507},"STM32F103C8T6",{"type":12,"tag":492,"props":509,"children":510},{},[511],{"type":18,"value":512},"Arduino Nano ESP32",{"type":12,"tag":492,"props":514,"children":515},{},[516],{"type":18,"value":517},"Integrate Bluetooth communication module, reduce the complexity of the design",{"type":12,"tag":442,"props":519,"children":520},{},[521,532,537,542],{"type":12,"tag":492,"props":522,"children":523},{},[524],{"type":12,"tag":453,"props":525,"children":526},{},[527],{"type":12,"tag":457,"props":528,"children":529},{},[530],{"type":18,"value":531},"Temperature Control",{"type":12,"tag":492,"props":533,"children":534},{},[535],{"type":18,"value":536},"TB6612FNG",{"type":12,"tag":492,"props":538,"children":539},{},[540],{"type":18,"value":541},"TCM1040",{"type":12,"tag":492,"props":543,"children":544},{},[545],{"type":18,"value":546},"More precise control of temperature",{"type":12,"tag":442,"props":548,"children":549},{},[550,561,566,571],{"type":12,"tag":492,"props":551,"children":552},{},[553],{"type":12,"tag":453,"props":554,"children":555},{},[556],{"type":12,"tag":457,"props":557,"children":558},{},[559],{"type":18,"value":560},"Fluorescence Detection",{"type":12,"tag":492,"props":562,"children":563},{},[564],{"type":18,"value":565},"AS7341",{"type":12,"tag":492,"props":567,"children":568},{},[569],{"type":18,"value":570},"FM-4A",{"type":12,"tag":492,"props":572,"children":573},{},[574],{"type":18,"value":575},"Integrate excitation light and fluorescent sensor to make the detection result more stable.",{"type":12,"tag":442,"props":577,"children":578},{},[579,590,595,600],{"type":12,"tag":492,"props":580,"children":581},{},[582],{"type":12,"tag":453,"props":583,"children":584},{},[585],{"type":12,"tag":457,"props":586,"children":587},{},[588],{"type":18,"value":589},"Reaction Platform",{"type":12,"tag":492,"props":591,"children":592},{},[593],{"type":18,"value":594},"Single Reaction Tube",{"type":12,"tag":492,"props":596,"children":597},{},[598],{"type":18,"value":599},"Slide Rail Platform",{"type":12,"tag":492,"props":601,"children":602},{},[603],{"type":18,"value":604},"Achieve high-throughput detection",{"type":12,"tag":42,"props":606,"children":607},{},[608],{"type":12,"tag":66,"props":609,"children":612},{"className":610},[611],"tab-title",[613],{"type":18,"value":614},"Table 1. Scheme difference",{"type":12,"tag":42,"props":616,"children":617},{},[618],{"type":18,"value":619},"​\nIn terms of material selection, we have adopted a black silicone kit and an aluminum alloy temperature-controlled slot as the environment for the centrifuge tubes. This scheme can excellently meet the insulation requirements of the design.",{"type":12,"tag":42,"props":621,"children":622},{},[623],{"type":12,"tag":57,"props":624,"children":627},{"alt":625,"src":626},"Edition Scheme Process","https://static.igem.wiki/teams/5358/engineering-success/edition-scheme-process.png",[],{"type":12,"tag":42,"props":629,"children":630},{},[631],{"type":12,"tag":66,"props":632,"children":634},{"className":633},[69],[635],{"type":18,"value":636},"Figure 8. Edition Scheme Process",{"type":12,"tag":28,"props":638,"children":640},{"id":639},"establishment",[641],{"type":18,"value":642},"Establishment",{"type":12,"tag":42,"props":644,"children":645},{},[646],{"type":18,"value":647},"​The system construction utilized common integrated modules, with the main controller being an ESP32 chip, and temperature control and detection were accomplished using the TCM temperature control module and LIDE Technology's FM series digital fluorescence detection module, respectively. We employed industrial processing techniques to fabricate the aluminum alloy temperature-controlled slots, while the casing and other components were created using 3D printing technology.",{"type":12,"tag":42,"props":649,"children":650},{},[651],{"type":12,"tag":57,"props":652,"children":655},{"alt":653,"src":654},"Schematic Diagram of Hardware Equipment","https://static.igem.wiki/teams/5358/engineering-success/new.png",[],{"type":12,"tag":42,"props":657,"children":658},{},[659],{"type":12,"tag":66,"props":660,"children":662},{"className":661},[69],[663],{"type":18,"value":664},"Figure 9. Schematic Diagram of Hardware Equipment",{"type":12,"tag":42,"props":666,"children":667},{},[668],{"type":12,"tag":57,"props":669,"children":672},{"alt":670,"src":671},"Model Diagram of Hardware Equipment","https://static.igem.wiki/teams/5358/engineering-success/model-diagram-of-hardware-equipment.png",[],{"type":12,"tag":42,"props":674,"children":675},{},[676],{"type":12,"tag":66,"props":677,"children":679},{"className":678},[69],[680],{"type":18,"value":681},"Figure 10. Model Diagram of Hardware Equipment",{"type":12,"tag":28,"props":683,"children":685},{"id":684},"test-4",[686],{"type":18,"value":110},{"type":12,"tag":42,"props":688,"children":689},{},[690],{"type":18,"value":691},"​After finalizing the initial plan, we first carried out feasibility tests to verify the functionality of the fluorescence system and the temperature control system. After assembling the initial version of the model, numerous issues were identified through usage testing and preliminary risk analysis, such as the susceptibility of the fluorescence system to interference and the instability of the temperature control system. Through a cycle of testing, feedback, improvement, and retesting, the final version of the model demonstrated excellent performance across all aspects. Experiments showed that fluorescence identification was highly accurate, and the overall aesthetics were significantly enhanced.",{"type":12,"tag":42,"props":693,"children":694},{},[695],{"type":12,"tag":57,"props":696,"children":699},{"alt":697,"src":698},"Results of Temperature Control Experiment","https://static.igem.wiki/teams/5358/engineering-success/results-of-temperature-control-experiment.png",[],{"type":12,"tag":42,"props":701,"children":702},{},[703],{"type":12,"tag":66,"props":704,"children":706},{"className":705},[69],[707],{"type":18,"value":708},"Figure 11. Results of Temperature Control Experiment",{"type":12,"tag":42,"props":710,"children":711},{},[712],{"type":12,"tag":57,"props":713,"children":716},{"alt":714,"src":715},"Results of Flurescence Verification Experiment","https://static.igem.wiki/teams/5358/engineering-success/results-of-flurescence-verification-experiment.png",[],{"type":12,"tag":42,"props":718,"children":719},{},[720],{"type":12,"tag":66,"props":721,"children":723},{"className":722},[69],[724],{"type":18,"value":725},"Figure 12. Results of Flurescence Verification Experiment",{"type":12,"tag":28,"props":727,"children":729},{"id":728},"learn-4",[730],{"type":18,"value":143},{"type":12,"tag":42,"props":732,"children":733},{},[734],{"type":18,"value":735},"​During the process of designing the device, we encountered various challenges, such as overly complex control systems, insufficient stability, inability to conduct multi-sample testing, and issues with the optical path design. Throughout the real-time testing and discussion phases, we continuously documented various issues, proposed improvement plans, and actively sought help from knowledgeable teachers and consultants. Eventually, we completed a hardware design that met the requirements. Through continuous learning, improvement, and questioning, our expertise in the relevant fields has been greatly boosted.",{"type":12,"tag":13,"props":737,"children":739},{"id":738},"software",[740],{"type":18,"value":741},"Software",{"type":12,"tag":28,"props":743,"children":745},{"id":744},"objectives-1",[746],{"type":18,"value":402},{"type":12,"tag":404,"props":748,"children":749},{},[750,755,760],{"type":12,"tag":408,"props":751,"children":752},{},[753],{"type":18,"value":754},"To implement human-machine interaction for information exchange with hardware.",{"type":12,"tag":408,"props":756,"children":757},{},[758],{"type":18,"value":759},"To automatically process data sent by the hardware.",{"type":12,"tag":408,"props":761,"children":762},{},[763],{"type":18,"value":764},"To visualize data.",{"type":12,"tag":28,"props":766,"children":768},{"id":767},"design-5",[769],{"type":18,"value":40},{"type":12,"tag":42,"props":771,"children":772},{},[773],{"type":18,"value":774},"​The main body is divided into two parts—the Bluetooth reception module and the plotting module.",{"type":12,"tag":28,"props":776,"children":778},{"id":777},"bluetooth-module-receives-data",[779],{"type":18,"value":780},"Bluetooth module receives data",{"type":12,"tag":782,"props":783,"children":785},"pre",{"code":784},"CREATE discoveryAgent AS new BluetoothDeviceDiscoveryAgent\n​\nCONNECT discoveryAgent.deviceDiscovered TO this.deviceDiscovered\nCONNECT discoveryAgent.finished TO this.onDeviceConnected\n​\n// Start searching for Bluetooth devices\nCALL discoveryAgent.start()\n​\n// Initialize Bluetooth Socket\nCREATE socket AS new BluetoothSocket USING RfcommProtocol\n​\n// Connect button click signal to send STARTSTART command\nCONNECT ui.pushButton.clicked TO this.sendStartSignal\n",[786],{"type":12,"tag":787,"props":788,"children":789},"code",{"__ignoreMap":5},[790],{"type":18,"value":784},{"type":12,"tag":28,"props":792,"children":794},{"id":793},"plotting-module-performs-data-visualization",[795],{"type":18,"value":796},"Plotting module performs data visualization",{"type":12,"tag":782,"props":798,"children":800},{"code":799},"FUNCTION updateGraphs()\n  // Create canvas\n  CREATE new scene\n  SET scene to graphicsView\n​\n  // Draw temperature graph\n  FOR i FROM 1 TO size of temperatureData - 1 DO\n    ADD line FROM (i-1, temperatureData[i-1]) TO (i, temperatureData[i]) WITH color red\n​\n  // Draw fluorescence intensity graph\n  FOR i FROM 1 TO size of fluorescenceData - 1 DO\n    ADD line FROM (i-1, fluorescenceData[i-1]) TO (i, fluorescenceData[i]) WITH color blue\n​\n  UPDATE graphicsView\nEND FUNCTION\n",[801],{"type":12,"tag":787,"props":802,"children":803},{"__ignoreMap":5},[804],{"type":18,"value":799},{"type":12,"tag":28,"props":806,"children":808},{"id":807},"data-segmentation-module",[809],{"type":18,"value":810},"Data segmentation module",{"type":12,"tag":782,"props":812,"children":814},{"code":813},"FUNCTION splitAndFindMax(dataPoints, zeroThresholdCount)\n    INITIALIZE maxValues AS empty list\n    INITIALIZE currentSegment AS empty list\n    SET zeroCount TO 0\n​\n    FOR each point IN dataPoints DO\n        IF point.value == 0.0 THEN\n            INCREMENT zeroCount\n            IF zeroCount >= zeroThresholdCount THEN\n                IF currentSegment is not empty THEN\n                    SET maxVal TO maximum value in currentSegment\n                    ADD maxVal TO maxValues\n                    CLEAR currentSegment\n                    SET zeroCount TO 0  // Reset the counter​\n                CONTINUE  // Skip the current point, do not include in currentSegment​\n            END IF\n        ELSE\n            SET zeroCount TO 0  // Reset the counter because a non-zero value was encountered\n        END IF\n​\n        //Add the current point to the currentSegment\n        ADD point TO currentSegment\n    END FOR\n​\n    // Process the last segment\n    IF currentSegment is not empty THEN\n        SET maxVal TO maximum value in currentSegment\n        ADD maxVal TO maxValues\n    END IF\n    RETURN maxValues\nEND FUNCTION\n",[815],{"type":12,"tag":787,"props":816,"children":817},{"__ignoreMap":5},[818],{"type":18,"value":813},{"type":12,"tag":28,"props":820,"children":822},{"id":821},"testing",[823],{"type":18,"value":824},"Testing",{"title":5,"searchDepth":826,"depth":827,"links":828},1,3,[829,841,848],{"id":15,"depth":830,"text":19,"children":831},2,[832,836],{"id":23,"depth":827,"text":26,"children":833},[834],{"id":30,"depth":835,"text":33},4,{"id":156,"depth":827,"text":159,"children":837},[838,839,840],{"id":162,"depth":835,"text":165},{"id":257,"depth":835,"text":260},{"id":325,"depth":835,"text":328},{"id":393,"depth":830,"text":396,"children":842},[843,844,845,846,847],{"id":399,"depth":835,"text":402},{"id":425,"depth":835,"text":40},{"id":639,"depth":835,"text":642},{"id":684,"depth":835,"text":110},{"id":728,"depth":835,"text":143},{"id":738,"depth":830,"text":741,"children":849},[850,851,852,853,854,855],{"id":744,"depth":835,"text":402},{"id":767,"depth":835,"text":40},{"id":777,"depth":835,"text":780},{"id":793,"depth":835,"text":796},{"id":807,"depth":835,"text":810},{"id":821,"depth":835,"text":824},"markdown","content:project-engineering.md","content","project-engineering.md","project-engineering","md",1727871092297]
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.el-button--info:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button{--el-button-font-weight:var(--el-font-weight-primary);--el-button-border-color:var(--el-border-color);--el-button-bg-color:var(--el-fill-color-blank);--el-button-text-color:var(--el-text-color-regular);--el-button-disabled-text-color:var(--el-disabled-text-color);--el-button-disabled-bg-color:var(--el-fill-color-blank);--el-button-disabled-border-color:var(--el-border-color-light);--el-button-divide-border-color:hsla(0,0%,100%,.5);--el-button-hover-text-color:var(--el-color-primary);--el-button-hover-bg-color:var(--el-color-primary-light-9);--el-button-hover-border-color:var(--el-color-primary-light-7);--el-button-active-text-color:var(--el-button-hover-text-color);--el-button-active-border-color:var(--el-color-primary);--el-button-active-bg-color:var(--el-button-hover-bg-color);--el-button-outline-color:var(--el-color-primary-light-5);--el-button-hover-link-text-color:var(--el-color-info);--el-button-active-color:var(--el-text-color-primary);align-items:center;-webkit-appearance:none;background-color:var(--el-button-bg-color);border:var(--el-border);border-color:var(--el-button-border-color);box-sizing:border-box;color:var(--el-button-text-color);cursor:pointer;display:inline-flex;font-weight:var(--el-button-font-weight);height:32px;justify-content:center;line-height:1;outline:none;text-align:center;transition:.1s;-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-button:hover{background-color:var(--el-button-hover-bg-color);border-color:var(--el-button-hover-border-color);color:var(--el-button-hover-text-color);outline:none}.el-button:active{background-color:var(--el-button-active-bg-color);border-color:var(--el-button-active-border-color);color:var(--el-button-active-text-color);outline:none}.el-button:focus-visible{outline:2px 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1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper 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.el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags 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.el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large 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11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) 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cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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79.1666666667%;max-width:79.1666666667%}.el-col-xs-19.is-guttered{display:block}.el-col-xs-offset-19{margin-left:79.1666666667%}.el-col-xs-pull-19{position:relative;right:79.1666666667%}.el-col-xs-push-19{left:79.1666666667%;position:relative}.el-col-xs-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xs-20.is-guttered{display:block}.el-col-xs-offset-20{margin-left:83.3333333333%}.el-col-xs-pull-20{position:relative;right:83.3333333333%}.el-col-xs-push-20{left:83.3333333333%;position:relative}.el-col-xs-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xs-21.is-guttered{display:block}.el-col-xs-offset-21{margin-left:87.5%}.el-col-xs-pull-21{position:relative;right:87.5%}.el-col-xs-push-21{left:87.5%;position:relative}.el-col-xs-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 0%;max-width:0}.el-col-sm-0.is-guttered{display:none}.el-col-sm-offset-0{margin-left:0}.el-col-sm-pull-0{position:relative;right:0}.el-col-sm-push-0{left:0;position:relative}.el-col-sm-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-sm-1,.el-col-sm-1.is-guttered{display:block}.el-col-sm-offset-1{margin-left:4.1666666667%}.el-col-sm-pull-1{position:relative;right:4.1666666667%}.el-col-sm-push-1{left:4.1666666667%;position:relative}.el-col-sm-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-sm-2,.el-col-sm-2.is-guttered{display:block}.el-col-sm-offset-2{margin-left:8.3333333333%}.el-col-sm-pull-2{position:relative;right:8.3333333333%}.el-col-sm-push-2{left:8.3333333333%;position:relative}.el-col-sm-3{flex:0 0 12.5%;max-width:12.5%}.el-col-sm-3,.el-col-sm-3.is-guttered{display:block}.el-col-sm-offset-3{margin-left:12.5%}.el-col-sm-pull-3{position:relative;right:12.5%}.el-col-sm-push-3{left:12.5%;position:relative}.el-col-sm-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-sm-4,.el-col-sm-4.is-guttered{display:block}.el-col-sm-offset-4{margin-left:16.6666666667%}.el-col-sm-pull-4{position:relative;right:16.6666666667%}.el-col-sm-push-4{left:16.6666666667%;position:relative}.el-col-sm-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-sm-5,.el-col-sm-5.is-guttered{display:block}.el-col-sm-offset-5{margin-left:20.8333333333%}.el-col-sm-pull-5{position:relative;right:20.8333333333%}.el-col-sm-push-5{left:20.8333333333%;position:relative}.el-col-sm-6{flex:0 0 25%;max-width:25%}.el-col-sm-6,.el-col-sm-6.is-guttered{display:block}.el-col-sm-offset-6{margin-left:25%}.el-col-sm-pull-6{position:relative;right:25%}.el-col-sm-push-6{left:25%;position:relative}.el-col-sm-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 0%;max-width:0}.el-col-xl-0.is-guttered{display:none}.el-col-xl-offset-0{margin-left:0}.el-col-xl-pull-0{position:relative;right:0}.el-col-xl-push-0{left:0;position:relative}.el-col-xl-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-xl-1,.el-col-xl-1.is-guttered{display:block}.el-col-xl-offset-1{margin-left:4.1666666667%}.el-col-xl-pull-1{position:relative;right:4.1666666667%}.el-col-xl-push-1{left:4.1666666667%;position:relative}.el-col-xl-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-xl-2,.el-col-xl-2.is-guttered{display:block}.el-col-xl-offset-2{margin-left:8.3333333333%}.el-col-xl-pull-2{position:relative;right:8.3333333333%}.el-col-xl-push-2{left:8.3333333333%;position:relative}.el-col-xl-3{flex:0 0 12.5%;max-width:12.5%}.el-col-xl-3,.el-col-xl-3.is-guttered{display:block}.el-col-xl-offset-3{margin-left:12.5%}.el-col-xl-pull-3{position:relative;right:12.5%}.el-col-xl-push-3{left:12.5%;position:relative}.el-col-xl-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-xl-4,.el-col-xl-4.is-guttered{display:block}.el-col-xl-offset-4{margin-left:16.6666666667%}.el-col-xl-pull-4{position:relative;right:16.6666666667%}.el-col-xl-push-4{left:16.6666666667%;position:relative}.el-col-xl-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-xl-5,.el-col-xl-5.is-guttered{display:block}.el-col-xl-offset-5{margin-left:20.8333333333%}.el-col-xl-pull-5{position:relative;right:20.8333333333%}.el-col-xl-push-5{left:20.8333333333%;position:relative}.el-col-xl-6{flex:0 0 25%;max-width:25%}.el-col-xl-6,.el-col-xl-6.is-guttered{display:block}.el-col-xl-offset-6{margin-left:25%}.el-col-xl-pull-6{position:relative;right:25%}.el-col-xl-push-6{left:25%;position:relative}.el-col-xl-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-xl-10.is-guttered{display:block}.el-col-xl-offset-10{margin-left:41.6666666667%}.el-col-xl-pull-10{position:relative;right:41.6666666667%}.el-col-xl-push-10{left:41.6666666667%;position:relative}.el-col-xl-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-xl-11.is-guttered{display:block}.el-col-xl-offset-11{margin-left:45.8333333333%}.el-col-xl-pull-11{position:relative;right:45.8333333333%}.el-col-xl-push-11{left:45.8333333333%;position:relative}.el-col-xl-12{display:block;flex:0 0 50%;max-width:50%}.el-col-xl-12.is-guttered{display:block}.el-col-xl-offset-12{margin-left:50%}.el-col-xl-pull-12{position:relative;right:50%}.el-col-xl-push-12{left:50%;position:relative}.el-col-xl-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px;box-sizing:border-box;height:12px;position:relative;width:280px}.el-color-alpha-slider__bar{background:linear-gradient(to right,hsla(0,0%,100%,0) 0,var(--el-bg-color) 100%);height:100%;position:relative}.el-color-alpha-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-alpha-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-picker.is-focused .el-color-picker__trigger{border-color:var(--el-color-primary)}.el-color-picker.is-disabled .el-color-picker__trigger{cursor:not-allowed}.el-color-picker--large{height:40px}.el-color-picker--large .el-color-picker__trigger{height:40px;width:40px}.el-color-picker--large .el-color-picker__mask{height:38px;width:38px}.el-color-picker--small{height:24px}.el-color-picker--small .el-color-picker__trigger{height:24px;width:24px}.el-color-picker--small .el-color-picker__mask{height:22px;width:22px}.el-color-picker--small .el-color-picker__empty,.el-color-picker--small .el-color-picker__icon{transform:scale(.8)}.el-color-picker__mask{background-color:hsla(0,0%,100%,.7);border-radius:4px;cursor:not-allowed;height:30px;left:1px;position:absolute;top:1px;width:30px;z-index:1}.el-color-picker__trigger{align-items:center;border:1px solid var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker 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5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid 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var(--el-transition-duration)}.dialog-fade-leave-active .el-overlay-dialog{animation:dialog-fade-out var(--el-transition-duration)}@keyframes dialog-fade-in{0%{opacity:0;transform:translate3d(0,-20px,0)}to{opacity:1;transform:translateZ(0)}}@keyframes dialog-fade-out{0%{opacity:1;transform:translateZ(0)}to{opacity:0;transform:translate3d(0,-20px,0)}}@keyframes modal-fade-in{0%{opacity:0}to{opacity:1}}@keyframes modal-fade-out{0%{opacity:1}to{opacity:0}}.el-divider{position:relative}.el-divider--horizontal{border-top:1px var(--el-border-color) var(--el-border-style);display:block;height:1px;margin:24px 0;width:100%}.el-divider--vertical{border-left:1px var(--el-border-color) var(--el-border-style);display:inline-block;height:1em;margin:0 8px;position:relative;vertical-align:middle;width:1px}.el-divider__text{background-color:var(--el-bg-color);color:var(--el-text-color-primary);font-size:14px;font-weight:500;padding:0 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.ttb{transform:translate(0)}.el-drawer__sr-focus:focus{outline:none!important}.el-drawer__header{align-items:center;color:#72767b;display:flex;margin-bottom:32px;padding:var(--el-drawer-padding-primary);padding-bottom:0}.el-drawer__header>:first-child{flex:1}.el-drawer__title{flex:1;font-size:16px;line-height:inherit;margin:0}.el-drawer__footer{padding:var(--el-drawer-padding-primary);padding-top:10px;text-align:right}.el-drawer__close-btn{background-color:transparent;border:none;color:inherit;cursor:pointer;display:inline-flex;font-size:var(--el-font-size-extra-large);outline:none}.el-drawer__close-btn:focus i,.el-drawer__close-btn:hover 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.el-popper__arrow:before{border:1px solid var(--el-border-color-light)}.el-dropdown__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-dropdown__popper .el-dropdown-menu{border:none}.el-dropdown__popper .el-dropdown__popper-selfdefine{outline:none}.el-dropdown__popper .el-scrollbar__bar{z-index:calc(var(--el-dropdown-menu-index) + 1)}.el-dropdown__popper .el-dropdown__list{box-sizing:border-box;list-style:none;margin:0;padding:0}.el-dropdown 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var(--el-transition-duration);width:var(--el-notification-width);z-index:9999}.el-notification.right{right:16px}.el-notification.left{left:16px}.el-notification__group{margin-left:var(--el-notification-group-margin-left);margin-right:var(--el-notification-group-margin-right)}.el-notification__title{color:var(--el-notification-title-color);font-size:var(--el-notification-title-font-size);font-weight:700;line-height:var(--el-notification-icon-size);margin:0}.el-notification__content{color:var(--el-notification-content-color);font-size:var(--el-notification-content-font-size);line-height:24px;margin:6px 0 0}.el-notification__content p{margin:0}.el-notification .el-notification__icon{font-size:var(--el-notification-icon-size);height:var(--el-notification-icon-size);width:var(--el-notification-icon-size)}.el-notification .el-notification__closeBtn{color:var(--el-notification-close-color);cursor:pointer;font-size:var(--el-notification-close-font-size);position:absolute;right:15px;top:18px}.el-notification .el-notification__closeBtn:hover{color:var(--el-notification-close-hover-color)}.el-notification .el-notification--success{--el-notification-icon-color:var(--el-color-success);color:var(--el-notification-icon-color)}.el-notification .el-notification--info{--el-notification-icon-color:var(--el-color-info);color:var(--el-notification-icon-color)}.el-notification .el-notification--warning{--el-notification-icon-color:var(--el-color-warning);color:var(--el-notification-icon-color)}.el-notification .el-notification--error{--el-notification-icon-color:var(--el-color-error);color:var(--el-notification-icon-color)}.el-notification-fade-enter-from.right{right:0;transform:translateX(100%)}.el-notification-fade-enter-from.left{left:0;transform:translateX(-100%)}.el-notification-fade-leave-to{opacity:0}.el-overlay{background-color:var(--el-overlay-color-lighter);bottom:0;height:100%;left:0;overflow:auto;position:fixed;right:0;top:0;z-index:2000}.el-overlay .el-overlay-root{height:0}.el-page-header.is-contentful .el-page-header__main{border-top:1px solid var(--el-border-color-light);margin-top:16px}.el-page-header__header{align-items:center;display:flex;justify-content:space-between;line-height:24px}.el-page-header__left{align-items:center;display:flex;margin-right:40px;position:relative}.el-page-header__back{align-items:center;cursor:pointer;display:flex}.el-page-header__left .el-divider--vertical{margin:0 16px}.el-page-header__icon{align-items:center;display:flex;font-size:16px;margin-right:10px}.el-page-header__icon .el-icon{font-size:inherit}.el-page-header__title{font-size:14px;font-weight:500}.el-page-header__content{color:var(--el-text-color-primary);font-size:18px}.el-page-header__breadcrumb{margin-bottom:16px}.el-pagination{--el-pagination-font-size:14px;--el-pagination-bg-color:var(--el-fill-color-blank);--el-pagination-text-color:var(--el-text-color-primary);--el-pagination-border-radius:2px;--el-pagination-button-color:var(--el-text-color-primary);--el-pagination-button-width:32px;--el-pagination-button-height:32px;--el-pagination-button-disabled-color:var(--el-text-color-placeholder);--el-pagination-button-disabled-bg-color:var(--el-fill-color-blank);--el-pagination-button-bg-color:var(--el-fill-color);--el-pagination-hover-color:var(--el-color-primary);--el-pagination-font-size-small:12px;--el-pagination-button-width-small:24px;--el-pagination-button-height-small:24px;--el-pagination-button-width-large:40px;--el-pagination-button-height-large:40px;--el-pagination-item-gap:16px;align-items:center;color:var(--el-pagination-text-color);display:flex;font-size:var(--el-pagination-font-size);font-weight:400;white-space:nowrap}.el-pagination .el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small 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.el-transfer-panel__header{align-items:center;background:var(--el-transfer-panel-header-bg-color);border:1px solid var(--el-transfer-border-color);border-top-left-radius:var(--el-transfer-border-radius);border-top-right-radius:var(--el-transfer-border-radius);box-sizing:border-box;color:var(--el-color-black);display:flex;height:var(--el-transfer-panel-header-height);margin:0;padding-left:15px}.el-transfer-panel .el-transfer-panel__header .el-checkbox{align-items:center;display:flex;position:relative;width:100%}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label{color:var(--el-text-color-primary);font-size:16px;font-weight:400}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label span{color:var(--el-text-color-secondary);font-size:12px;font-weight:400;position:absolute;right:15px;top:50%;transform:translate3d(0,-50%,0)}.el-transfer-panel .el-transfer-panel__footer{background:var(--el-bg-color-overlay);border:1px solid 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0;overflow:hidden;padding:0;width:var(--el-upload-list-picture-card-size)}.el-upload-list--picture-card .el-upload-list__item .el-icon--check,.el-upload-list--picture-card .el-upload-list__item .el-icon--circle-check{color:#fff}.el-upload-list--picture-card .el-upload-list__item .el-icon--close{display:none}.el-upload-list--picture-card .el-upload-list__item:hover .el-upload-list__item-status-label{display:block;opacity:0}.el-upload-list--picture-card .el-upload-list__item:hover .el-progress__text{display:block}.el-upload-list--picture-card .el-upload-list__item .el-upload-list__item-name{display:none}.el-upload-list--picture-card .el-upload-list__item-thumbnail{height:100%;-o-object-fit:contain;object-fit:contain;width:100%}.el-upload-list--picture-card .el-upload-list__item-status-label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-list--picture-card .el-upload-list__item-status-label 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img{display:block;height:100%;width:100%}.el-upload-cover__label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-cover__label i{color:#fff;font-size:12px;margin-top:11px;transform:rotate(-45deg)}.el-upload-cover__progress{display:inline-block;position:static;vertical-align:middle;width:243px}.el-upload-cover__progress+.el-upload__inner{opacity:0}.el-upload-cover__content{height:100%;left:0;position:absolute;top:0;width:100%}.el-upload-cover__interact{background-color:var(--el-overlay-color-light);bottom:0;height:100%;left:0;position:absolute;text-align:center;width:100%}.el-upload-cover__interact .btn{color:#fff;cursor:pointer;display:inline-block;font-size:14px;margin-top:60px;transition:var(--el-transition-md-fade);vertical-align:middle}.el-upload-cover__interact .btn i{margin-top:0}.el-upload-cover__interact .btn span{opacity:0;transition:opacity .15s linear}.el-upload-cover__interact 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var(--el-border-color-light)}.el-popper.is-light>.el-popper__arrow:before{right:0}.el-popper.is-pure{padding:0}.el-popper__arrow,.el-popper__arrow:before{height:10px;position:absolute;width:10px;z-index:-1}.el-popper__arrow:before{background:var(--el-text-color-primary);box-sizing:border-box;content:" 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srcset="https://static.igem.wiki/teams/5358/banner/project.webp 1x, https://static.igem.wiki/teams/5358/banner/project.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/project.webp"><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg">Engineering</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[200px] md:mr-10"><h1 class="mt-0">TOC</h1><ul><!--[--><li class="my-5"><a class="cursor-pointer">Biosensor</a><ul class="hidden pl-5 pt-2"><!--[--><!--[--><li class="text-[#fff] font-normal mb-2"><a class="cursor-pointer">Luminescence</a></li><!--]--><!--[--><li class="text-[#fff] font-normal mb-2"><a class="cursor-pointer">AND Gate Design</a></li><!--]--><!--]--></ul></li><li class="my-5"><a class="cursor-pointer">Hardware</a><ul class="hidden pl-5 pt-2"><!--[--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--]--></ul></li><li class="my-5"><a class="cursor-pointer">Software</a><ul class="hidden pl-5 pt-2"><!--[--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--]--></ul></li><!--]--></ul></aside><div class="content"><h2 id="biosensor"><!--[-->Biosensor<!--]--></h2><h3 id="luminescence"><!--[-->Luminescence<!--]--></h3><h4 id="circle-design-reca-bsfbfp"><!--[-->Circle: Design recA-BsFbFp<!--]--></h4><h5 id="design"><!--[-->Design<!--]--></h5><p><!--[-->​In order to adapt to the low oxygen environment of the hardware design, we replaced the egfp gene, which strictly requires oxygen molecules as a cofactor for its luminescence, with the reporter gene BsFbFp. This gene can effectively express fluorescent signals in low or anaerobic environments.<!--]--></p><h5 id="build"><!--[-->Build<!--]--></h5><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="BsFbFp circuits" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/bsfbfp-circuits.png 1x, https://static.igem.wiki/teams/5358/engineering-success/bsfbfp-circuits.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/bsfbfp-circuits.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 1. BsFbFp circuits</span><!--]--></p><p><!--[-->​	Genetic circuit: Gene recA and BsFbFp were all synthesized by Sangon Biotech according to BIOBRICK standard, and the gene circuit was obtained by PCR and verified by agarose gel electrophoresis.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="build results of BsFbFp " data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/build-results-of-bsfbfp.png 1x, https://static.igem.wiki/teams/5358/engineering-success/build-results-of-bsfbfp.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/build-results-of-bsfbfp.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 2. Build results of BsFbfP</span><!--]--></p><p><!--[-->​Construction of plasmid: The results of the agarose gel electrophoresis successfully showed bands for recA, the vector and BsFbFp, confirming that the gene circuit was successfully connected to the vector.<!--]--></p><p><!--[-->​Ideal Strain: The successfully constructed plasmid was transformed into BL21 competent cells and cultured on LB solid medium with ampicillin.<!--]--></p><h5 id="test"><!--[-->Test<!--]--></h5><p><!--[-->​Adding 600μL of bacterial solution and 30μL of ampicillin to 30mL of fresh culture medium, logarithmic growth was performed. Then, NA (Nalidixic Acid) was added in a gradient based on concentration to induce the bacteria. Subsequently, 250μL from each group was added to a anaerobic chamber and incubated on a shaking incubator at 37°C with a speed of 180 r/min. After 2 hours, the OD value and fluorescence intensity were measured.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="RFU-BsFbFp in 10lianpaichip(anaerobic chamber)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/rfu-bsfbfp-in-10lianpaichip-anaerobic-chamber.jpg 1x, https://static.igem.wiki/teams/5358/engineering-success/rfu-bsfbfp-in-10lianpaichip-anaerobic-chamber.jpg 2x" src="https://static.igem.wiki/teams/5358/engineering-success/rfu-bsfbfp-in-10lianpaichip-anaerobic-chamber.jpg"><!--]--></p><p><!--[--><span class="fig-title">Figure 3. RFU-BsFbFp in 10lianpaichip(anaerobic chamber)</span><!--]--></p><p><!--[-->​The RFU (Relative Fluorescence Units) of the three groups of engineered bacteria increased linearly with the amount of NA (Nalidixic Acid) added. The results indicate that the fluorescent expression circuit of BsFbFp has been successfully constructed, and the engineered bacteria can effectively express fluorescence in an anaerobic environment.<!--]--></p><h5 id="learn"><!--[-->Learn<!--]--></h5><p><!--[-->​In conclusion，the plasmid constructed using BsFbFp as a reporter gene can effectively express fluorescence in low-oxygen or anaerobic environments, which is beneficial for the design of the hardware components.<!--]--></p><p><!--[-->​So we believed that the work in this cycle has been successfully accomplished., and we obtained the engineered bacteria needed for our goals.<!--]--></p><h3 id="and-gate-design"><!--[-->AND Gate Design<!--]--></h3><h4 id="circle-1-design-pet28-reca-hrp-egfp"><!--[-->Circle 1: Design pET28-recA-HRP-egfp<!--]--></h4><h5 id="design-1"><!--[-->Design<!--]--></h5><p><!--[-->​To construct the and gate, we introduced the hrp system, composed of the hrpR gene and hrpS gene, as well as the HrpL promoter regulated by the sigma54 factor. The hrpL promoter will only be activated when both the hrpR gene and the hrpS gene are expressed simultaneously.<!--]--></p><p><!--[-->​In 2023, the BIT team successfully constructed P recA, the Hrp system, and egfp on the pUC19 plasmid. However, tests showed that the expression level of the circuit constructed using the pUC19 plasmid as a vector was relatively low, significantly deviating from the expected results. Therefore, we plan to switch to the pET28 plasmid, which has a more prominent expression level, to facilitate the synergistic assessment of endocrine disruption effects and oxidative damage effects.<!--]--></p><h5 id="build-1"><!--[-->Build<!--]--></h5><p><!--[-->​Genetic circuit: recA-HRP-egfp were synthesized by Sangon Biotech according to BIOBRICK standard， the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Cir1-1" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/cir1-1.png 1x, https://static.igem.wiki/teams/5358/engineering-success/cir1-1.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/cir1-1.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 4. enzyme digestion result of circle 1</span><!--]--></p><p><!--[-->​
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role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Human Practices<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li></ul><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-mobile px-10 flex lg:hidden"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 2x" class="cursor-pointer py-2" src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div class="grow"></div><!--[--><span class="el-avatar el-avatar--icon el-avatar--circle self-center cursor-pointer el-tooltip__trigger el-tooltip__trigger" style=""><i class="el-icon" style=""><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M160 448a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zM160 896a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32z"></path></svg><!--]--></i></span><!--teleport start--><!--teleport end--><!--]--></ul></header><div class="pt-[8rem]"><!--[--><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="cover" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/project.webp 1x, https://static.igem.wiki/teams/5358/banner/project.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/project.webp"><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg">Engineering</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[200px] md:mr-10"><h1 class="mt-0">TOC</h1><ul><!--[--><li class="my-5"><a class="cursor-pointer">Biosensor</a><ul class="hidden pl-5 pt-2"><!--[--><!--[--><li class="text-[#fff] font-normal mb-2"><a class="cursor-pointer">Luminescence</a></li><!--]--><!--[--><li class="text-[#fff] font-normal mb-2"><a class="cursor-pointer">AND Gate Design</a></li><!--]--><!--]--></ul></li><li class="my-5"><a class="cursor-pointer">Hardware</a><ul class="hidden pl-5 pt-2"><!--[--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--]--></ul></li><li class="my-5"><a class="cursor-pointer">Software</a><ul class="hidden pl-5 pt-2"><!--[--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--[--><!----><!--]--><!--]--></ul></li><!--]--></ul></aside><div class="content"><h2 id="biosensor"><!--[-->Biosensor<!--]--></h2><h3 id="luminescence"><!--[-->Luminescence<!--]--></h3><h4 id="circle-design-reca-bsfbfp"><!--[-->Circle: Design recA-BsFbFp<!--]--></h4><h5 id="design"><!--[-->Design<!--]--></h5><p><!--[-->​In order to adapt to the low oxygen environment of the hardware design, we replaced the egfp gene, which strictly requires oxygen molecules as a cofactor for its luminescence, with the reporter gene BsFbFp. This gene can effectively express fluorescent signals in low or anaerobic environments.<!--]--></p><h5 id="build"><!--[-->Build<!--]--></h5><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="BsFbFp circuits" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/bsfbfp-circuits.png 1x, https://static.igem.wiki/teams/5358/engineering-success/bsfbfp-circuits.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/bsfbfp-circuits.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 1. BsFbFp circuits</span><!--]--></p><p><!--[-->​Genetic circuit: Gene recA and BsFbFp were all synthesized by Sangon Biotech according to BIOBRICK standard, and the gene circuit was obtained by PCR and verified by agarose gel electrophoresis.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="build results of BsFbFp " data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/build-results-of-bsfbfp.png 1x, https://static.igem.wiki/teams/5358/engineering-success/build-results-of-bsfbfp.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/build-results-of-bsfbfp.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 2. Build results of BsFbfP</span><!--]--></p><p><!--[-->​Construction of plasmid: The results of the agarose gel electrophoresis successfully showed bands for recA, the vector and BsFbFp, confirming that the gene circuit was successfully connected to the vector.<!--]--></p><p><!--[-->​Ideal Strain: The successfully constructed plasmid was transformed into BL21 competent cells and cultured on LB solid medium with ampicillin.<!--]--></p><h5 id="test"><!--[-->Test<!--]--></h5><p><!--[-->​Adding 600μL of bacterial solution and 30μL of ampicillin to 30mL of fresh culture medium, logarithmic growth was performed. Then, NA (Nalidixic Acid) was added in a gradient based on concentration to induce the bacteria. Subsequently, 250μL from each group was added to a anaerobic chamber and incubated on a shaking incubator at 37°C with a speed of 180 r/min. After 2 hours, the OD value and fluorescence intensity were measured.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="RFU-BsFbFp in 10lianpaichip(anaerobic chamber)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/rfu-bsfbfp-in-10lianpaichip-anaerobic-chamber.jpg 1x, https://static.igem.wiki/teams/5358/engineering-success/rfu-bsfbfp-in-10lianpaichip-anaerobic-chamber.jpg 2x" src="https://static.igem.wiki/teams/5358/engineering-success/rfu-bsfbfp-in-10lianpaichip-anaerobic-chamber.jpg"><!--]--></p><p><!--[--><span class="fig-title">Figure 3. RFU-BsFbFp in 10lianpaichip(anaerobic chamber)</span><!--]--></p><p><!--[-->​The RFU (Relative Fluorescence Units) of the three groups of engineered bacteria increased linearly with the amount of NA (Nalidixic Acid) added. The results indicate that the fluorescent expression circuit of BsFbFp has been successfully constructed, and the engineered bacteria can effectively express fluorescence in an anaerobic environment.<!--]--></p><h5 id="learn"><!--[-->Learn<!--]--></h5><p><!--[-->​In conclusion，the plasmid constructed using BsFbFp as a reporter gene can effectively express fluorescence in low-oxygen or anaerobic environments, which is beneficial for the design of the hardware components.<!--]--></p><p><!--[-->​So we believed that the work in this cycle has been successfully accomplished., and we obtained the engineered bacteria needed for our goals.<!--]--></p><h3 id="and-gate-design"><!--[-->AND Gate Design<!--]--></h3><h4 id="circle-1-design-pet28-reca-hrp-egfp"><!--[-->Circle 1: Design pET28-recA-HRP-egfp<!--]--></h4><h5 id="design-1"><!--[-->Design<!--]--></h5><p><!--[-->​To construct the and gate, we introduced the hrp system, composed of the hrpR gene and hrpS gene, as well as the HrpL promoter regulated by the sigma54 factor. The hrpL promoter will only be activated when both the hrpR gene and the hrpS gene are expressed simultaneously.<!--]--></p><p><!--[-->​In 2023, the BIT team successfully constructed P recA, the Hrp system, and egfp on the pUC19 plasmid. However, tests showed that the expression level of the circuit constructed using the pUC19 plasmid as a vector was relatively low, significantly deviating from the expected results. Therefore, we plan to switch to the pET28 plasmid, which has a more prominent expression level, to facilitate the synergistic assessment of endocrine disruption effects and oxidative damage effects.<!--]--></p><h5 id="build-1"><!--[-->Build<!--]--></h5><p><!--[-->​Genetic circuit: recA-HRP-egfp were synthesized by Sangon Biotech according to BIOBRICK standard， the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Cir1-1" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/cir1-1.png 1x, https://static.igem.wiki/teams/5358/engineering-success/cir1-1.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/cir1-1.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 4. enzyme digestion result of circle 1</span><!--]--></p><p><!--[-->​
 The results of the agarose gel electrophoresis indicated that the vector was successfully digested and the target fragment was successfully amplified by PCR. The ligation was carried out at a 1:4 ratio of vector to target fragment, followed by transformation into DH5α competent cells and verification through agarose gel electrophoresis.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Cir1-2" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/cir1-2.png 1x, https://static.igem.wiki/teams/5358/engineering-success/cir1-2.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/cir1-2.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 5. build result of circle 1</span><!--]--></p><p><!--[-->​
 Verification results: In multiple rounds of construction validation experiments, the target fragment showed no band in the agarose gel electrophoresis results, and the restriction enzyme digestion verification indicated self-ligation of the vector, proving that the construction in this cycle has failed.<!--]--></p><h5 id="test-1"><!--[-->Test<!--]--></h5><p><!--[-->​Due to the failure of the target plasmid construction in this cycle, it is necessary to analyze the issues present in the plasmid design and construction process and make corrections.<!--]--></p><h5 id="learn-1"><!--[-->Learn<!--]--></h5><p><!--[-->​During this construction process, we overlooked the reagent concentration ratio of the vector to the target fragment in our ligation system, which may be a major factor contributing to the failure of ligation. We plan to introduce the concentration factor of the ligation reagents into the ratio to improve the efficiency of ligation.<!--]--></p><h4 id="circle-2-adjust-the-ligation-system-and-reconstruct-the-plasmid-pet28-reca-hrp-egfp"><!--[-->Circle 2: Adjust the ligation system and reconstruct the plasmid pET28-recA-HRP-egfp.<!--]--></h4><h5 id="design-2"><!--[-->Design<!--]--></h5><p><!--[-->​Based on the experience learned from Circle 1, we plan to use a new ligation system, increase the dosage of the target fragment, and improve the ligation efficiency.<!--]--></p><h5 id="build-2"><!--[-->Build<!--]--></h5><p><!--[-->​The construction process used three different ligation systems, with the ratios of vector to target fragment being 1:4, 1:5, and 1:6, respectively. The constructed plasmids were extracted and verified by PCR followed by agarose gel electrophoresis.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Cir2" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/cir2.png 1x, https://static.igem.wiki/teams/5358/engineering-success/cir2.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/cir2.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 6. built result of circle 2</span><!--]--></p><p><!--[-->​
 Construction of plasmid: In the multi-round adjustment experiments of the ligation system construction, the agarose gel electrophoresis results still did not show a band for the target fragment, indicating that the construction in this cycle was unsuccessful.<!--]--></p><h5 id="test-2"><!--[-->Test<!--]--></h5><p><!--[-->​Due to the failure of target plasmid construction in this cycle, testing cannot be carried out.<!--]--></p><h5 id="learn-2"><!--[-->Learn<!--]--></h5><p><!--[-->​In this cycle, we have corrected the ligation system, removing this influencing factor. Therefore, we suspect that the low partial digestion efficiency at the target sequence enzyme cutting sites may be the cause. This could be due to issues with the setting of protective bases near the enzyme cutting sites when designing the PCR primers, leading to another ligation failure. As such, we plan to redesign the PCR primers and increase the number of protective bases on both sides of the enzyme cutting sites from one or none to three to improve the success rate of the ligation.<!--]--></p><h4 id="circle-3-redesign-the-primers-increase-protective-bases-and-reconstruct-pet28-reca-hrp-egfp"><!--[-->Circle 3: Redesign the primers, increase protective bases, and reconstruct pET28-recA-HRP-egfp<!--]--></h4><h5 id="design-3"><!--[-->Design<!--]--></h5><p><!--[-->​Based on the experience learned from Circle 2, we have redesigned the PCR primers for the target fragment and added protective bases to them, and we are reconstructing the pET28-recA-HRP-egfp plasmid.<!--]--></p><h5 id="build-3"><!--[-->Build<!--]--></h5><p><!--[-->​After changing to new PCR primers and setting the protective bases on both sides of the restriction enzyme cutting sites to 3, the construction was carried out. Genetic circuits: recA-HRP-egfp were synthesized by Sangon Biotech according the BIOBRICK standard, the pET28 was obtained by enzyme digestion and the gene circuit was obtained by PCR.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Cir3" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/cir3.png 1x, https://static.igem.wiki/teams/5358/engineering-success/cir3.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/cir3.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 7. built result of circle 3</span><!--]--></p><p><!--[-->​
 The agarose gel electrophoresis results showed that after redesigning the PCR primers and increasing the number of protective bases on both sides of the restriction enzyme cutting sites from one or none to three, only one band was observed for the target fragment, indicating that the new PCR primers have strong specificity and the target fragment was successfully obtained.
-​	However, due to time constraints, we did not proceed with the subsequent experiments.<!--]--></p><h5 id="test-3"><!--[-->Test<!--]--></h5><p><!--[-->​Limited by time, we were unable to complete the reconstruction, and therefore have no test results.<!--]--></p><h5 id="learn-3"><!--[-->Learn<!--]--></h5><p><!--[-->​In this cycle, the new PCR primers and the addition of protective bases at both ends of the restriction enzyme sites are beneficial for improving the success rate of enzyme ligation, thereby providing an experimental basis for the successful construction of the gate circuit.<!--]--></p><h2 id="hardware"><!--[-->Hardware<!--]--></h2><h4 id="objectives"><!--[-->Objectives<!--]--></h4><ol><!--[--><li><!--[-->To provide a high-throughput portable reaction vessel.<!--]--></li><li><!--[-->To provide stable physical conditions for detection.<!--]--></li><li><!--[-->High-sensitivity fluorescence detection.<!--]--></li><!--]--></ol><h4 id="design-4"><!--[-->Design<!--]--></h4><p><!--[-->​Our entire device mainly consists of three major parts: the outer casing that encases the entire device, the reaction area located in the upper layer, and the detection area situated in the lower layer. In the initial design, we utilized an STM32 chip as the main controller to regulate temperature control, fluorescence detection, and Bluetooth communication functions. In the second iteration, we replaced the main controller with an ESP32 chip that integrates a wireless communication module, and we adopted integrated temperature control and detection modules, which further enhances the system&#39;s stability and accuracy. To meet the need for simultaneous detection of multiple samples, we also redesigned the high-throughput reaction chambers, achieving rapid successive detection through a linear slide rail system.<!--]--></p><table><!--[--><thead><!--[--><tr><!--[--><th><!--[--><!--]--></th><th><!--[--><em><!--[--><strong><!--[-->Initial Scheme<!--]--></strong><!--]--></em><!--]--></th><th><!--[--><em><!--[--><strong><!--[-->Final Scheme<!--]--></strong><!--]--></em><!--]--></th><th><!--[--><em><!--[--><strong><!--[-->Advantages After Improvement<!--]--></strong><!--]--></em><!--]--></th><!--]--></tr><!--]--></thead><tbody><!--[--><tr><!--[--><td><!--[--><em><!--[--><strong><!--[-->MCU<!--]--></strong><!--]--></em><!--]--></td><td><!--[-->STM32F103C8T6<!--]--></td><td><!--[-->Arduino Nano ESP32<!--]--></td><td><!--[-->Integrate Bluetooth communication module, reduce the complexity of the design<!--]--></td><!--]--></tr><tr><!--[--><td><!--[--><em><!--[--><strong><!--[-->Temperature Control<!--]--></strong><!--]--></em><!--]--></td><td><!--[-->TB6612FNG<!--]--></td><td><!--[-->TCM1040<!--]--></td><td><!--[-->More precise control of temperature<!--]--></td><!--]--></tr><tr><!--[--><td><!--[--><em><!--[--><strong><!--[-->Fluorescence Detection<!--]--></strong><!--]--></em><!--]--></td><td><!--[-->AS7341<!--]--></td><td><!--[-->FM-4A<!--]--></td><td><!--[-->Integrate excitation light and fluorescent sensor to make the detection result more stable.<!--]--></td><!--]--></tr><tr><!--[--><td><!--[--><em><!--[--><strong><!--[-->Reaction Platform<!--]--></strong><!--]--></em><!--]--></td><td><!--[-->Single Reaction Tube<!--]--></td><td><!--[-->Slide Rail Platform<!--]--></td><td><!--[-->Achieve high-throughput detection<!--]--></td><!--]--></tr><!--]--></tbody><!--]--></table><p><!--[--><span class="tab-title">Table 1. Scheme difference</span><!--]--></p><p><!--[-->​
-In terms of material selection, we have adopted a black silicone kit and an aluminum alloy temperature-controlled slot as the environment for the centrifuge tubes. This scheme can excellently meet the insulation requirements of the design.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Edition Scheme Process" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/edition-scheme-process.png 1x, https://static.igem.wiki/teams/5358/engineering-success/edition-scheme-process.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/edition-scheme-process.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 8. Edition Scheme Process</span><!--]--></p><h4 id="establishment"><!--[-->Establishment<!--]--></h4><p><!--[-->​	The system construction utilized common integrated modules, with the main controller being an ESP32 chip, and temperature control and detection were accomplished using the TCM temperature control module and LIDE Technology&#39;s FM series digital fluorescence detection module, respectively. We employed industrial processing techniques to fabricate the aluminum alloy temperature-controlled slots, while the casing and other components were created using 3D printing technology.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Schematic Diagram of Hardware Equipment" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/new.png 1x, https://static.igem.wiki/teams/5358/engineering-success/new.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/new.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 9. Schematic Diagram of Hardware Equipment</span><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Model Diagram of Hardware Equipment" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/model-diagram-of-hardware-equipment.png 1x, https://static.igem.wiki/teams/5358/engineering-success/model-diagram-of-hardware-equipment.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/model-diagram-of-hardware-equipment.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 10. Model Diagram of Hardware Equipment</span><!--]--></p><h4 id="test-4"><!--[-->Test<!--]--></h4><p><!--[-->​	After finalizing the initial plan, we first carried out feasibility tests to verify the functionality of the fluorescence system and the temperature control system. After assembling the initial version of the model, numerous issues were identified through usage testing and preliminary risk analysis, such as the susceptibility of the fluorescence system to interference and the instability of the temperature control system. Through a cycle of testing, feedback, improvement, and retesting, the final version of the model demonstrated excellent performance across all aspects. Experiments showed that fluorescence identification was highly accurate, and the overall aesthetics were significantly enhanced.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Results of Temperature Control Experiment" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/results-of-temperature-control-experiment.png 1x, https://static.igem.wiki/teams/5358/engineering-success/results-of-temperature-control-experiment.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/results-of-temperature-control-experiment.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 11. Results of Temperature Control Experiment</span><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Results of Flurescence Verification Experiment" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/results-of-flurescence-verification-experiment.png 1x, https://static.igem.wiki/teams/5358/engineering-success/results-of-flurescence-verification-experiment.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/results-of-flurescence-verification-experiment.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 12. Results of Flurescence Verification Experiment</span><!--]--></p><h4 id="learn-4"><!--[-->Learn<!--]--></h4><p><!--[-->​	During the process of designing the device, we encountered various challenges, such as overly complex control systems, insufficient stability, inability to conduct multi-sample testing, and issues with the optical path design. Throughout the real-time testing and discussion phases, we continuously documented various issues, proposed improvement plans, and actively sought help from knowledgeable teachers and consultants. Eventually, we completed a hardware design that met the requirements. Through continuous learning, improvement, and questioning, our expertise in the relevant fields has been greatly boosted.<!--]--></p><h2 id="software"><!--[-->Software<!--]--></h2><h4 id="objectives-1"><!--[-->Objectives<!--]--></h4><ol><!--[--><li><!--[-->To implement human-machine interaction for information exchange with hardware.<!--]--></li><li><!--[-->To automatically process data sent by the hardware.<!--]--></li><li><!--[-->To visualize data.<!--]--></li><!--]--></ol><h4 id="design-5"><!--[-->Design<!--]--></h4><p><!--[-->​The main body is divided into two parts—the Bluetooth reception module and the plotting module.<!--]--></p><h4 id="bluetooth-module-receives-data"><!--[-->Bluetooth module receives data<!--]--></h4><!--[--><pre class="" style=""><!--[--><code>CREATE discoveryAgent AS new BluetoothDeviceDiscoveryAgent
+​
+However, due to time constraints, we did not proceed with the subsequent experiments.<!--]--></p><h5 id="test-3"><!--[-->Test<!--]--></h5><p><!--[-->​Limited by time, we were unable to complete the reconstruction, and therefore have no test results.<!--]--></p><h5 id="learn-3"><!--[-->Learn<!--]--></h5><p><!--[-->​In this cycle, the new PCR primers and the addition of protective bases at both ends of the restriction enzyme sites are beneficial for improving the success rate of enzyme ligation, thereby providing an experimental basis for the successful construction of the gate circuit.<!--]--></p><h2 id="hardware"><!--[-->Hardware<!--]--></h2><h4 id="objectives"><!--[-->Objectives<!--]--></h4><ol><!--[--><li><!--[-->To provide a high-throughput portable reaction vessel.<!--]--></li><li><!--[-->To provide stable physical conditions for detection.<!--]--></li><li><!--[-->High-sensitivity fluorescence detection.<!--]--></li><!--]--></ol><h4 id="design-4"><!--[-->Design<!--]--></h4><p><!--[-->​Our entire device mainly consists of three major parts: the outer casing that encases the entire device, the reaction area located in the upper layer, and the detection area situated in the lower layer. In the initial design, we utilized an STM32 chip as the main controller to regulate temperature control, fluorescence detection, and Bluetooth communication functions. In the second iteration, we replaced the main controller with an ESP32 chip that integrates a wireless communication module, and we adopted integrated temperature control and detection modules, which further enhances the system&#39;s stability and accuracy. To meet the need for simultaneous detection of multiple samples, we also redesigned the high-throughput reaction chambers, achieving rapid successive detection through a linear slide rail system.<!--]--></p><table><!--[--><thead><!--[--><tr><!--[--><th><!--[--><!--]--></th><th><!--[--><em><!--[--><strong><!--[-->Initial Scheme<!--]--></strong><!--]--></em><!--]--></th><th><!--[--><em><!--[--><strong><!--[-->Final Scheme<!--]--></strong><!--]--></em><!--]--></th><th><!--[--><em><!--[--><strong><!--[-->Advantages After Improvement<!--]--></strong><!--]--></em><!--]--></th><!--]--></tr><!--]--></thead><tbody><!--[--><tr><!--[--><td><!--[--><em><!--[--><strong><!--[-->MCU<!--]--></strong><!--]--></em><!--]--></td><td><!--[-->STM32F103C8T6<!--]--></td><td><!--[-->Arduino Nano ESP32<!--]--></td><td><!--[-->Integrate Bluetooth communication module, reduce the complexity of the design<!--]--></td><!--]--></tr><tr><!--[--><td><!--[--><em><!--[--><strong><!--[-->Temperature Control<!--]--></strong><!--]--></em><!--]--></td><td><!--[-->TB6612FNG<!--]--></td><td><!--[-->TCM1040<!--]--></td><td><!--[-->More precise control of temperature<!--]--></td><!--]--></tr><tr><!--[--><td><!--[--><em><!--[--><strong><!--[-->Fluorescence Detection<!--]--></strong><!--]--></em><!--]--></td><td><!--[-->AS7341<!--]--></td><td><!--[-->FM-4A<!--]--></td><td><!--[-->Integrate excitation light and fluorescent sensor to make the detection result more stable.<!--]--></td><!--]--></tr><tr><!--[--><td><!--[--><em><!--[--><strong><!--[-->Reaction Platform<!--]--></strong><!--]--></em><!--]--></td><td><!--[-->Single Reaction Tube<!--]--></td><td><!--[-->Slide Rail Platform<!--]--></td><td><!--[-->Achieve high-throughput detection<!--]--></td><!--]--></tr><!--]--></tbody><!--]--></table><p><!--[--><span class="tab-title">Table 1. Scheme difference</span><!--]--></p><p><!--[-->​
+In terms of material selection, we have adopted a black silicone kit and an aluminum alloy temperature-controlled slot as the environment for the centrifuge tubes. This scheme can excellently meet the insulation requirements of the design.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Edition Scheme Process" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/edition-scheme-process.png 1x, https://static.igem.wiki/teams/5358/engineering-success/edition-scheme-process.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/edition-scheme-process.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 8. Edition Scheme Process</span><!--]--></p><h4 id="establishment"><!--[-->Establishment<!--]--></h4><p><!--[-->​The system construction utilized common integrated modules, with the main controller being an ESP32 chip, and temperature control and detection were accomplished using the TCM temperature control module and LIDE Technology&#39;s FM series digital fluorescence detection module, respectively. We employed industrial processing techniques to fabricate the aluminum alloy temperature-controlled slots, while the casing and other components were created using 3D printing technology.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Schematic Diagram of Hardware Equipment" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/new.png 1x, https://static.igem.wiki/teams/5358/engineering-success/new.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/new.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 9. Schematic Diagram of Hardware Equipment</span><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Model Diagram of Hardware Equipment" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/model-diagram-of-hardware-equipment.png 1x, https://static.igem.wiki/teams/5358/engineering-success/model-diagram-of-hardware-equipment.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/model-diagram-of-hardware-equipment.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 10. Model Diagram of Hardware Equipment</span><!--]--></p><h4 id="test-4"><!--[-->Test<!--]--></h4><p><!--[-->​After finalizing the initial plan, we first carried out feasibility tests to verify the functionality of the fluorescence system and the temperature control system. After assembling the initial version of the model, numerous issues were identified through usage testing and preliminary risk analysis, such as the susceptibility of the fluorescence system to interference and the instability of the temperature control system. Through a cycle of testing, feedback, improvement, and retesting, the final version of the model demonstrated excellent performance across all aspects. Experiments showed that fluorescence identification was highly accurate, and the overall aesthetics were significantly enhanced.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Results of Temperature Control Experiment" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/results-of-temperature-control-experiment.png 1x, https://static.igem.wiki/teams/5358/engineering-success/results-of-temperature-control-experiment.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/results-of-temperature-control-experiment.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 11. Results of Temperature Control Experiment</span><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="Results of Flurescence Verification Experiment" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/results-of-flurescence-verification-experiment.png 1x, https://static.igem.wiki/teams/5358/engineering-success/results-of-flurescence-verification-experiment.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/results-of-flurescence-verification-experiment.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 12. Results of Flurescence Verification Experiment</span><!--]--></p><h4 id="learn-4"><!--[-->Learn<!--]--></h4><p><!--[-->​During the process of designing the device, we encountered various challenges, such as overly complex control systems, insufficient stability, inability to conduct multi-sample testing, and issues with the optical path design. Throughout the real-time testing and discussion phases, we continuously documented various issues, proposed improvement plans, and actively sought help from knowledgeable teachers and consultants. Eventually, we completed a hardware design that met the requirements. Through continuous learning, improvement, and questioning, our expertise in the relevant fields has been greatly boosted.<!--]--></p><h2 id="software"><!--[-->Software<!--]--></h2><h4 id="objectives-1"><!--[-->Objectives<!--]--></h4><ol><!--[--><li><!--[-->To implement human-machine interaction for information exchange with hardware.<!--]--></li><li><!--[-->To automatically process data sent by the hardware.<!--]--></li><li><!--[-->To visualize data.<!--]--></li><!--]--></ol><h4 id="design-5"><!--[-->Design<!--]--></h4><p><!--[-->​The main body is divided into two parts—the Bluetooth reception module and the plotting module.<!--]--></p><h4 id="bluetooth-module-receives-data"><!--[-->Bluetooth module receives data<!--]--></h4><!--[--><pre class="" style=""><!--[--><code>CREATE discoveryAgent AS new BluetoothDeviceDiscoveryAgent
 ​
 CONNECT discoveryAgent.deviceDiscovered TO this.deviceDiscovered
 CONNECT discoveryAgent.finished TO this.onDeviceConnected
@@ -116,5 +117,5 @@ END FUNCTION
     END IF
     RETURN maxValues
 END FUNCTION
-</code><!--]--></pre><!--]--><h4 id="testing"><!--[-->Testing<!--]--></h4></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a aria-current="page" href="/bit/engineering" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/engineering/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-UXJZW5OGcq":11},null,"/engineering",1727868994393]</script>
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\ No newline at end of file
+</code><!--]--></pre><!--]--><h4 id="testing"><!--[-->Testing<!--]--></h4></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a aria-current="page" href="/bit/engineering" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/engineering/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-UXJZW5OGcq":11},null,"/engineering",1727871091263]</script>
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diff --git a/.output/public/experiment/_payload.json b/.output/public/experiment/_payload.json
index 4fa0ed0d519e47c0094337ffa696793362c12b1b..900d74b07cfbb37ce3d2160c28fd4195aec6995d 100644
--- a/.output/public/experiment/_payload.json
+++ b/.output/public/experiment/_payload.json
@@ -1 +1 @@
-[{"data":1,"prerenderedAt":704},["Reactive",2],{"content-query-yAJtGgOdxJ":3},{"_path":4,"_dir":5,"_draft":6,"_partial":6,"_locale":5,"title":7,"description":5,"body":8,"_type":698,"_id":699,"_source":700,"_file":701,"_stem":702,"_extension":703},"/wetlab-experiment","",false,"Experiment",{"type":9,"children":10,"toc":679},"root",[11,20,26,32,37,42,53,63,68,82,87,92,97,117,126,130,135,139,147,170,178,183,226,232,237,243,247,252,256,261,353,359,363,368,372,377,475,481,486,492,496,501,505,533,539,545,550,556,561,566,646,652,658,663,669,674],{"type":12,"tag":13,"props":14,"children":16},"element","h2",{"id":15},"what-is-endocrine-pollutant",[17],{"type":18,"value":19},"text","What IS Endocrine Pollutant？",{"type":12,"tag":21,"props":22,"children":23},"p",{},[24],{"type":18,"value":25},"Endocrine pollutants, also known as endocrine-disrupting chemicals (EDCs), are substances that can interfere with the normal functioning of the endocrine system. These chemicals mimic, block, or alter the effects of hormones in the body, potentially leading to adverse health effects. The endocrine system, which includes glands such as the thyroid, adrenal, and pituitary, is responsible for releasing hormones that regulate many vital functions, including growth, metabolism, and reproduction.",{"type":12,"tag":13,"props":27,"children":29},{"id":28},"what-is-dna-damage",[30],{"type":18,"value":31},"What IS DNA Damage？",{"type":12,"tag":21,"props":33,"children":34},{},[35],{"type":18,"value":36},"DNA damage refers to alterations in the chemical structure of DNA, which can affect its function and integrity. This damage can occur through various mechanisms and can lead to a range of cellular consequences if not properly repaired.",{"type":12,"tag":13,"props":38,"children":40},{"id":39},"experiment",[41],{"type":18,"value":7},{"type":12,"tag":43,"props":44,"children":46},"h3",{"id":45},"strain-obtain",[47],{"type":12,"tag":48,"props":49,"children":50},"strong",{},[51],{"type":18,"value":52},"Strain Obtain",{"type":12,"tag":54,"props":55,"children":57},"h4",{"id":56},"gene-acquisition",[58],{"type":12,"tag":48,"props":59,"children":60},{},[61],{"type":18,"value":62},"gene acquisition",{"type":12,"tag":21,"props":64,"children":65},{},[66],{"type":18,"value":67},"The recA，rbs30，hrpR，hrpS，PhrpL ，eGFP，BsFbFp，PgapA，T7RNAP，VAMER intein，PT7，LuxI and LuxR genes were synthesized by Sangon Biotech according to the standard of biobrick, and the line fragments were obtained through PCR.",{"type":12,"tag":69,"props":70,"children":72},"h5",{"id":71},"pcr",[73],{"type":12,"tag":74,"props":75,"children":76},"em",{},[77],{"type":12,"tag":48,"props":78,"children":79},{},[80],{"type":18,"value":81},"PCR",{"type":12,"tag":21,"props":83,"children":84},{},[85],{"type":18,"value":86},"Material",{"type":12,"tag":21,"props":88,"children":89},{},[90],{"type":18,"value":91},"Thermocycler, (0.2ml)PCR tube, pipetting gun, pipette tip, 2xPCRmix, upstream primer, downstream primer, sterile water, DNA template.",{"type":12,"tag":21,"props":93,"children":94},{},[95],{"type":18,"value":96},"Protocol",{"type":12,"tag":98,"props":99,"children":100},"ul",{},[101,107,112],{"type":12,"tag":102,"props":103,"children":104},"li",{},[105],{"type":18,"value":106},"Add to PCR tube (the total system volume: 25 μL): 12.5 μL 2×PCRmix, 9 μL of sterile water, 1.5 μl DNA template, 1 μL reverse primer, 1 μL forward primer;",{"type":12,"tag":102,"props":108,"children":109},{},[110],{"type":18,"value":111},"Put the PCR tube into the thermocycler and execute the program: pre-denaturation at 94°C for 210s; followed by 30 cycles of denaturation at 94°C for 30s, anneal at the range of 52-62°C for 30s, extension at 72°C for 60s;and a final extension at 72°C for 5 min;",{"type":12,"tag":102,"props":113,"children":114},{},[115],{"type":18,"value":116},"The product is stored at -10°C;",{"type":12,"tag":69,"props":118,"children":120},{"id":119},"agarose-gel-electrophoresis-and-sample-recovery",[121],{"type":12,"tag":48,"props":122,"children":123},{},[124],{"type":18,"value":125},"Agarose gel electrophoresis and sample recovery",{"type":12,"tag":21,"props":127,"children":128},{},[129],{"type":18,"value":86},{"type":12,"tag":21,"props":131,"children":132},{},[133],{"type":18,"value":134},"Electric hot water bath, electronic balance, electrophoresis instrument, gel imaging analysis system, pipetting gun, pipette tip, glass bottle, agarose, electrophoresis buffer, nucleic acid dye, DNA loading buffer, DNA precipitation buffer, gel buffer, elution buffer;",{"type":12,"tag":21,"props":136,"children":137},{},[138],{"type":18,"value":96},{"type":12,"tag":21,"props":140,"children":141},{},[142],{"type":12,"tag":48,"props":143,"children":144},{},[145],{"type":18,"value":146},"Agarose gel electrophoresis",{"type":12,"tag":98,"props":148,"children":149},{},[150,155,160,165],{"type":12,"tag":102,"props":151,"children":152},{},[153],{"type":18,"value":154},"2% agarose solution: add 30mL of 1X TAE electrophoresis buffer into a glass bottle. then weigh 0.6g of agarose and add it into the bottle;",{"type":12,"tag":102,"props":156,"children":157},{},[158],{"type":18,"value":159},"Heat the mixture(2% agarose solution) to boiling. Add 3μL of fluorescent dye to the heated liquid and mix it completely. Take out the glue box and place a mold in the box. Then pour the mixture into the mold and wait for solidification at room temperature;",{"type":12,"tag":102,"props":161,"children":162},{},[163],{"type":18,"value":164},"Remove the cooled and formed agarose gel and place it in the electrophoresis instrument. Mix the DNA samples with 1/5 of DNA precipitation buffer thoroughly. Then inject the standard samples and the mixture into different holes of the gel. Turn on the electrophoresis instrument and adjust the voltage to 110V for 40 min;",{"type":12,"tag":102,"props":166,"children":167},{},[168],{"type":18,"value":169},"Take out the agarose gel and open the gel imaging analysis instrument to analyze and save the electrophoresis results.",{"type":12,"tag":21,"props":171,"children":172},{},[173],{"type":12,"tag":48,"props":174,"children":175},{},[176],{"type":18,"value":177},"Sample recovery",{"type":12,"tag":21,"props":179,"children":180},{},[181],{"type":18,"value":182},"The electrophoresis products were purified by gel recovery method, and the purified materials were using the DNA gel recovery kit of Axygen . The operation steps are as follows :",{"type":12,"tag":98,"props":184,"children":185},{},[186,191,196,201,206,211,216,221],{"type":12,"tag":102,"props":187,"children":188},{},[189],{"type":18,"value":190},"Open the water bath in advance and set the temperature to 75°C(167°F);",{"type":12,"tag":102,"props":192,"children":193},{},[194],{"type":18,"value":195},"Use Agarose gel electrophoresis to verify the DNA fragment and observe in the gel imaging system. Cut the agarose gel containing the target band with a knife under the ultraviolet lamp, and record the weight of the 1.5 mL centrifuge tube in advance. After the centrifuge tube is loaded, weigh it again and calculate the gel weight. 100 mg is a gel volume equivalent to 100 μL;",{"type":12,"tag":102,"props":197,"children":198},{},[199],{"type":18,"value":200},"Add 3 gel volume of gel buffer DE-A, mix well and put into 75°C water bath heating 6-8 min, mix it up and down every 2 min during water bath;",{"type":12,"tag":102,"props":202,"children":203},{},[204],{"type":18,"value":205},"Add 0.5 DE-A volume of gel buffer DE-B, uniform mix it, and add 1 gel volume of isopropanol;",{"type":12,"tag":102,"props":207,"children":208},{},[209],{"type":18,"value":210},"Purify the resulting products by centrifugation. Transfer the above solution to an adsorption column and place it in a 2mL centrifuge tube. Centrifuge at 12,000 rpm for 1 min, discard the waste liquid, and place the adsorption column back into the collection tube;",{"type":12,"tag":102,"props":212,"children":213},{},[214],{"type":18,"value":215},"Add 500 μL buffer W1 to the adsorption column, centrifuge at 12,000 rpm for 30s, pour the waste liquid, and place the adsorption column back into the collection tube;",{"type":12,"tag":102,"props":217,"children":218},{},[219],{"type":18,"value":220},"Add 700 μL buffer W2 to the adsorption column, centrifuge at 12,000 rpm for 30s, pour the waste liquid and place the adsorption column back into the collection tube, and repeat the operation once again;",{"type":12,"tag":102,"props":222,"children":223},{},[224],{"type":18,"value":225},"Put the adsorption column in a new 1.5ml centrifuge tube, add 30μl Eluent in the center of the adsorption column membrane, and then centrifuge at the 12,000rpm for 1min after standing at room temperature, from which electrophoretic products were 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After incubation, plate 100μL of the transformed cells onto LB agar plates containing 100μg/mL ampicillin resistance. Invert the plates and incubate overnight at 37°C. Pick single colonies and inoculate them into 30mL of antibiotic-free LB broth. Add 30μL of ampicillin and incubate at 37°C with shaking at 180r/min for 12 h. Preserve the cultures in glycerol at -10°C;",{"type":12,"tag":69,"props":487,"children":489},{"id":488},"plasmid-transformation",[490],{"type":18,"value":491},"Plasmid transformation",{"type":12,"tag":21,"props":493,"children":494},{},[495],{"type":18,"value":86},{"type":12,"tag":21,"props":497,"children":498},{},[499],{"type":18,"value":500},"Vertical clean bench, shaker, water bath, centrifuge, pipetting gun, pipette tip, solid culture dish, alcohol lamp, applicator, ampicillin, LB-free liquid medium, competent cells;",{"type":12,"tag":21,"props":502,"children":503},{},[504],{"type":18,"value":96},{"type":12,"tag":98,"props":506,"children":507},{},[508,513,518,523,528],{"type":12,"tag":102,"props":509,"children":510},{},[511],{"type":18,"value":512},"Fill the foam box with about 2/3 of the volume of ice, and take100μL of prepared competent cells. turn on the ultra-clean table UV lamp in advance for 15min for sterilization. After sterilization, light up the alcohol lamp in the ultra-clean table, and transfer 50μL competent cells into a new aseptic centrifuge tube with a pipette beside the alcohol lamp, and add 5μL plasmid solution into it.",{"type":12,"tag":102,"props":514,"children":515},{},[516],{"type":18,"value":517},"place the centrifuge tube containing the plasmid solution in the ice box for 30min, turn on the water bath in advance and set the temperature at 42°C, heat the centrifuge tube after the ice bath in the water bath at 42°C for 45s, and immediately remove the centrifuge tube and place it in the ice box for 2min to complete the transformation process;",{"type":12,"tag":102,"props":519,"children":520},{},[521],{"type":18,"value":522},"Take out the packaged liquid medium without anti-LB, and add 500μL liquid medium into the centrifuge tube after heating. Incubate the centrifuge tube in a shaking table at 37°C 180r/min for 1h;",{"type":12,"tag":102,"props":524,"children":525},{},[526],{"type":18,"value":527},"After removing the centrifuge tube from the shaker, centrifuge it at 7000rpm for 30 s. Carefully aspirate and discard the supernatant (400μL) next to an alcohol lamp. Resuspend the remaining bacterial pellet using a pipetting gun, and based on plasmid resistance, select solid medium containing ampicillin resistance. Using a pipetting gun next to an alcohol lamp, add 100μL of the resuspended bacterial culture onto the surface of the solid medium. Spread evenly using a spreader. Cover the petri dish and invert it, then place it in a 37°C incubator for 12 h;",{"type":12,"tag":102,"props":529,"children":530},{},[531],{"type":18,"value":532},"Add 30μL of ampicillin to 30mL of antibiotic-free LB liquid medium. Using a pipette tip, pick a single colony and inoculate it into the aforementioned liquid medium. 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Measure OD600 and fluorescence intensity of bacterial solution by microplate reader;",{"type":12,"tag":262,"props":567,"children":568},{},[569,588],{"type":12,"tag":266,"props":570,"children":571},{},[572],{"type":12,"tag":270,"props":573,"children":574},{},[575,580,585],{"type":12,"tag":274,"props":576,"children":577},{},[578],{"type":18,"value":579},"DNA damaging reagent",{"type":12,"tag":274,"props":581,"children":582},{},[583],{"type":18,"value":584},"Concentration gradient",{"type":12,"tag":274,"props":586,"children":587},{},[],{"type":12,"tag":285,"props":589,"children":590},{},[591],{"type":12,"tag":270,"props":592,"children":593},{},[594,599,604],{"type":12,"tag":292,"props":595,"children":596},{},[597],{"type":18,"value":598},"NA",{"type":12,"tag":292,"props":600,"children":601},{},[602],{"type":18,"value":603},"0μL",{"type":12,"tag":292,"props":605,"children":606},{},[607],{"type":18,"value":608},"1μL 5μL 10μL 15μL 20μL 25μL",{"type":12,"tag":54,"props":610,"children":612},{"id":611},"data-measurement",[613],{"type":18,"value":614},"Data measurement",{"type":12,"tag":69,"props":616,"children":618},{"id":617},"measure-the-od600",[619],{"type":18,"value":620},"Measure the OD600",{"type":12,"tag":21,"props":622,"children":623},{},[624],{"type":18,"value":625},"Transfer 200μL of the bacteria solution to a 96-hole black microplate and place it in a microplate reader. 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Measure the fluorescence intensity.",{"type":12,"tag":21,"props":638,"children":639},{},[640],{"type":18,"value":641},"Each group of data was measured three times and averaged.",{"title":5,"searchDepth":643,"depth":644,"links":645},1,3,[646,648,649],{"id":15,"depth":647,"text":19},2,{"id":28,"depth":647,"text":31},{"id":39,"depth":647,"text":7,"children":650},[651,656],{"id":45,"depth":644,"text":52,"children":652},[653,655],{"id":56,"depth":654,"text":62},4,{"id":228,"depth":654,"text":231},{"id":535,"depth":644,"text":538,"children":657},[658,659,660],{"id":541,"depth":654,"text":544},{"id":552,"depth":654,"text":555},{"id":611,"depth":654,"text":614},"markdown","content:wetlab-experiment.md","content","wetlab-experiment.md","wetlab-experiment","md",1727871092318]
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diff --git a/.output/public/experiment/index.html b/.output/public/experiment/index.html
index 1a96b56bae6fe26fbe22690e3c788b89c6703cb2..d655dc797d8a2c2a5ff6693d4e042d8ec6c6b683 100644
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.el-alert__description{color:var(--el-color-info)}.el-alert--info.is-dark{background-color:var(--el-color-info);color:var(--el-color-white)}.el-alert--warning{--el-alert-bg-color:var(--el-color-warning-light-9)}.el-alert--warning.is-light{background-color:var(--el-alert-bg-color)}.el-alert--warning.is-light,.el-alert--warning.is-light .el-alert__description{color:var(--el-color-warning)}.el-alert--warning.is-dark{background-color:var(--el-color-warning);color:var(--el-color-white)}.el-alert--error{--el-alert-bg-color:var(--el-color-error-light-9)}.el-alert--error.is-light{background-color:var(--el-alert-bg-color)}.el-alert--error.is-light,.el-alert--error.is-light .el-alert__description{color:var(--el-color-error)}.el-alert--error.is-dark{background-color:var(--el-color-error);color:var(--el-color-white)}.el-alert__content{display:flex;flex-direction:column;gap:4px}.el-alert .el-alert__icon{font-size:var(--el-alert-icon-size);margin-right:8px;width:var(--el-alert-icon-size)}.el-alert 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inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;display:inline-block;position:relative;width:var(--el-input-width)}.el-autocomplete__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-autocomplete__popper.el-popper,.el-autocomplete__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-border-color-light)}.el-autocomplete__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-autocomplete__popper.el-popper[data-popper-placement^=bottom] 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translateX(100%);z-index:var(--el-index-normal)}.el-badge__content.is-fixed.is-dot{right:5px}.el-badge__content.is-dot{border-radius:50%;height:8px;padding:0;right:0;width:8px}.el-badge__content--primary{background-color:var(--el-color-primary)}.el-badge__content--success{background-color:var(--el-color-success)}.el-badge__content--warning{background-color:var(--el-color-warning)}.el-badge__content--info{background-color:var(--el-color-info)}.el-badge__content--danger{background-color:var(--el-color-danger)}.el-breadcrumb{font-size:14px;line-height:1}.el-breadcrumb:after,.el-breadcrumb:before{content:"";display:table}.el-breadcrumb:after{clear:both}.el-breadcrumb__separator{color:var(--el-text-color-placeholder);font-weight:700;margin:0 9px}.el-breadcrumb__separator.el-icon{font-weight:400;margin:0 6px}.el-breadcrumb__separator.el-icon 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.el-button--info:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button{--el-button-font-weight:var(--el-font-weight-primary);--el-button-border-color:var(--el-border-color);--el-button-bg-color:var(--el-fill-color-blank);--el-button-text-color:var(--el-text-color-regular);--el-button-disabled-text-color:var(--el-disabled-text-color);--el-button-disabled-bg-color:var(--el-fill-color-blank);--el-button-disabled-border-color:var(--el-border-color-light);--el-button-divide-border-color:hsla(0,0%,100%,.5);--el-button-hover-text-color:var(--el-color-primary);--el-button-hover-bg-color:var(--el-color-primary-light-9);--el-button-hover-border-color:var(--el-color-primary-light-7);--el-button-active-text-color:var(--el-button-hover-text-color);--el-button-active-border-color:var(--el-color-primary);--el-button-active-bg-color:var(--el-button-hover-bg-color);--el-button-outline-color:var(--el-color-primary-light-5);--el-button-hover-link-text-color:var(--el-color-info);--el-button-active-color:var(--el-text-color-primary);align-items:center;-webkit-appearance:none;background-color:var(--el-button-bg-color);border:var(--el-border);border-color:var(--el-button-border-color);box-sizing:border-box;color:var(--el-button-text-color);cursor:pointer;display:inline-flex;font-weight:var(--el-button-font-weight);height:32px;justify-content:center;line-height:1;outline:none;text-align:center;transition:.1s;-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-button:hover{background-color:var(--el-button-hover-bg-color);border-color:var(--el-button-hover-border-color);color:var(--el-button-hover-text-color);outline:none}.el-button:active{background-color:var(--el-button-active-bg-color);border-color:var(--el-button-active-border-color);color:var(--el-button-active-text-color);outline:none}.el-button:focus-visible{outline:2px 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20px;position:relative}.el-cascader-node.is-selectable.in-active-path{color:var(--el-cascader-menu-text-color)}.el-cascader-node.in-active-path,.el-cascader-node.is-active,.el-cascader-node.is-selectable.in-checked-path{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader-node:not(.is-disabled){cursor:pointer}.el-cascader-node:not(.is-disabled):focus,.el-cascader-node:not(.is-disabled):hover{background:var(--el-cascader-node-background-hover)}.el-cascader-node.is-disabled{color:var(--el-cascader-node-color-disabled);cursor:not-allowed}.el-cascader-node__prefix{left:10px;position:absolute}.el-cascader-node__postfix{position:absolute;right:10px}.el-cascader-node__label{flex:1;overflow:hidden;padding:0 8px;text-align:left;text-overflow:ellipsis;white-space:nowrap}.el-cascader-node>.el-checkbox,.el-cascader-node>.el-radio{margin-right:0}.el-cascader-node>.el-radio .el-radio__label{padding-left:0}.el-cascader{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);display:inline-block;font-size:var(--el-font-size-base);line-height:32px;outline:none;position:relative;vertical-align:middle}.el-cascader:not(.is-disabled):hover .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset;cursor:pointer}.el-cascader .el-input{cursor:pointer;display:flex}.el-cascader .el-input .el-input__inner{cursor:pointer;text-overflow:ellipsis}.el-cascader .el-input .el-input__suffix-inner .el-icon{height:calc(100% - 2px)}.el-cascader .el-input .el-input__suffix-inner .el-icon svg{vertical-align:middle}.el-cascader .el-input .icon-arrow-down{font-size:14px;transition:transform var(--el-transition-duration)}.el-cascader .el-input .icon-arrow-down.is-reverse{transform:rotate(180deg)}.el-cascader .el-input .icon-circle-close:hover{color:var(--el-input-clear-hover-color,var(--el-text-color-secondary))}.el-cascader .el-input.is-focus .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-focus-border-color,var(--el-color-primary)) inset}.el-cascader--large{font-size:14px;line-height:40px}.el-cascader--small{font-size:12px;line-height:24px}.el-cascader.is-disabled .el-cascader__label{color:var(--el-disabled-text-color);z-index:calc(var(--el-index-normal) + 1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper .el-popper__arrow:before{border:var(--el-cascader-menu-border)}.el-cascader__dropdown.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper{box-shadow:var(--el-cascader-menu-shadow)}.el-cascader__tags{box-sizing:border-box;display:flex;flex-wrap:wrap;left:0;line-height:normal;position:absolute;right:30px;text-align:left;top:50%;transform:translateY(-50%)}.el-cascader__tags .el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags .el-tag{align-items:center;background:var(--el-fill-color);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag.el-tag--dark,.el-cascader__collapse-tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__collapse-tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__collapse-tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__collapse-tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__suggestion-panel{border-radius:var(--el-cascader-menu-radius)}.el-cascader__suggestion-list{color:var(--el-cascader-menu-text-color);font-size:var(--el-font-size-base);margin:0;max-height:204px;padding:6px 0;text-align:center}.el-cascader__suggestion-item{align-items:center;cursor:pointer;display:flex;height:34px;justify-content:space-between;outline:none;padding:0 15px;text-align:left}.el-cascader__suggestion-item:focus,.el-cascader__suggestion-item:hover{background:var(--el-cascader-node-background-hover)}.el-cascader__suggestion-item.is-checked{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader__suggestion-item>span{margin-right:10px}.el-cascader__empty-text{color:var(--el-cascader-color-empty);margin:10px 0}.el-cascader__search-input{background:transparent;border:none;box-sizing:border-box;color:var(--el-cascader-menu-text-color);flex:1;height:24px;margin:2px 0 2px 11px;min-width:60px;outline:none;padding:0}.el-cascader__search-input::-moz-placeholder{color:transparent}.el-cascader__search-input::placeholder{color:transparent}.el-check-tag{background-color:var(--el-color-info-light-9);border-radius:var(--el-border-radius-base);color:var(--el-color-info);cursor:pointer;display:inline-block;font-size:var(--el-font-size-base);font-weight:700;line-height:var(--el-font-size-base);padding:7px 15px;transition:var(--el-transition-all)}.el-check-tag:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--primary.is-checked{background-color:var(--el-color-primary-light-8);color:var(--el-color-primary)}.el-check-tag.el-check-tag--primary.is-checked:hover{background-color:var(--el-color-primary-light-7)}.el-check-tag.el-check-tag--primary.is-checked.is-disabled{background-color:var(--el-color-primary-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-checked.is-disabled:hover{background-color:var(--el-color-primary-light-8)}.el-check-tag.el-check-tag--primary.is-disabled{background-color:var(--el-color-info-light-9);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-check-tag.el-check-tag--success.is-checked{background-color:var(--el-color-success-light-8);color:var(--el-color-success)}.el-check-tag.el-check-tag--success.is-checked:hover{background-color:var(--el-color-success-light-7)}.el-check-tag.el-check-tag--success.is-checked.is-disabled{background-color:var(--el-color-success-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-checked.is-disabled:hover{background-color:var(--el-color-success-light-8)}.el-check-tag.el-check-tag--success.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-disabled,.el-check-tag.el-check-tag--success.is-disabled:hover{background-color:var(--el-color-success-light-9)}.el-check-tag.el-check-tag--warning.is-checked{background-color:var(--el-color-warning-light-8);color:var(--el-color-warning)}.el-check-tag.el-check-tag--warning.is-checked:hover{background-color:var(--el-color-warning-light-7)}.el-check-tag.el-check-tag--warning.is-checked.is-disabled{background-color:var(--el-color-warning-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-checked.is-disabled:hover{background-color:var(--el-color-warning-light-8)}.el-check-tag.el-check-tag--warning.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-disabled,.el-check-tag.el-check-tag--warning.is-disabled:hover{background-color:var(--el-color-warning-light-9)}.el-check-tag.el-check-tag--danger.is-checked{background-color:var(--el-color-danger-light-8);color:var(--el-color-danger)}.el-check-tag.el-check-tag--danger.is-checked:hover{background-color:var(--el-color-danger-light-7)}.el-check-tag.el-check-tag--danger.is-checked.is-disabled{background-color:var(--el-color-danger-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-checked.is-disabled:hover{background-color:var(--el-color-danger-light-8)}.el-check-tag.el-check-tag--danger.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-disabled,.el-check-tag.el-check-tag--danger.is-disabled:hover{background-color:var(--el-color-danger-light-9)}.el-check-tag.el-check-tag--error.is-checked{background-color:var(--el-color-error-light-8);color:var(--el-color-error)}.el-check-tag.el-check-tag--error.is-checked:hover{background-color:var(--el-color-error-light-7)}.el-check-tag.el-check-tag--error.is-checked.is-disabled{background-color:var(--el-color-error-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-checked.is-disabled:hover{background-color:var(--el-color-error-light-8)}.el-check-tag.el-check-tag--error.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-disabled,.el-check-tag.el-check-tag--error.is-disabled:hover{background-color:var(--el-color-error-light-9)}.el-check-tag.el-check-tag--info.is-checked{background-color:var(--el-color-info-light-8);color:var(--el-color-info)}.el-check-tag.el-check-tag--info.is-checked:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--info.is-checked.is-disabled{background-color:var(--el-color-info-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-checked.is-disabled:hover{background-color:var(--el-color-info-light-8)}.el-check-tag.el-check-tag--info.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-disabled,.el-check-tag.el-check-tag--info.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-checkbox-button{--el-checkbox-button-checked-bg-color:var(--el-color-primary);--el-checkbox-button-checked-text-color:var(--el-color-white);--el-checkbox-button-checked-border-color:var(--el-color-primary);display:inline-block;position:relative}.el-checkbox-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left-color:transparent;bord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15px;position:relative;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-checkbox-button__inner.is-round{padding:8px 15px}.el-checkbox-button__inner:hover{color:var(--el-color-primary)}.el-checkbox-button__inner [class*=el-icon-]{line-height:.9}.el-checkbox-button__inner [class*=el-icon-]+span{margin-left:5px}.el-checkbox-button__original{margin:0;opacity:0;outline:none;position:absolute;z-index:-1}.el-checkbox-button.is-checked .el-checkbox-button__inner{background-color:var(--el-checkbox-button-checked-bg-color);border-color:var(--el-checkbox-button-checked-border-color);box-shadow:-1px 0 0 0 var(--el-color-primary-light-7);color:var(--el-checkbox-button-checked-text-color)}.el-checkbox-button.is-checked:first-child .el-checkbox-button__inner{border-left-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button.is-disabled .el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large .el-checkbox-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-checkbox-button--large .el-checkbox-button__inner.is-round{padding:12px 19px}.el-checkbox-button--small .el-checkbox-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-checkbox-button--small .el-checkbox-button__inner.is-round{padding:5px 11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) .el-checkbox__original:not(:focus-visible){border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__input.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-checked-icon-color);content:"";display:block;height:2px;left:0;position:absolute;right:0;top:5px;transform:scale(.5)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:after{display:none}.el-checkbox__inner{background-color:var(--el-checkbox-bg-color);border:var(--el-checkbox-input-border);border-radius:var(--el-checkbox-border-radius);box-sizing:border-box;display:inline-block;height:var(--el-checkbox-input-height);position:relative;transition:border-color .25s cubic-bezier(.71,-.46,.29,1.46),background-color .25s cubic-bezier(.71,-.46,.29,1.46),outline .25s cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 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54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 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var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid 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var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker .el-picker-panel__content{margin:0}.el-date-range-picker__header{height:28px;position:relative;text-align:center}.el-date-range-picker__header [class*=arrow-left]{float:left}.el-date-range-picker__header [class*=arrow-right]{float:right}.el-date-range-picker__header div{font-size:16px;font-weight:500;margin-right:50px}.el-date-range-picker__content{box-sizing:border-box;float:left;margin:0;padding:16px;width:50%}.el-date-range-picker__content.is-left{border-right:1px solid var(--el-datepicker-inner-border-color)}.el-date-range-picker__content .el-date-range-picker__header div{margin-left:50px;margin-right:50px}.el-date-range-picker__editors-wrap{box-sizing:border-box;display:table-cell}.el-date-range-picker__editors-wrap.is-right{text-align:right}.el-date-range-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid var(--el-timepicker-inner-border-color,var(--el-border-color-light));box-sizing:border-box;height:36px;line-height:25px;padding:4px;text-align:right}.el-time-panel__btn{background-color:transparent;border:none;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:28px;margin:0 5px;outline:none;padding:0 5px}.el-time-panel__btn.confirm{color:var(--el-timepicker-active-color,var(--el-color-primary));font-weight:800}.el-descriptions{--el-descriptions-table-border:1px solid var(--el-border-color-lighter);--el-descriptions-item-bordered-label-background:var(--el-fill-color-light);box-sizing:border-box;color:var(--el-text-color-primary);font-size:var(--el-font-size-base)}.el-descriptions__header{align-items:center;display:flex;justify-content:space-between;margin-bottom:16px}.el-descriptions__title{color:var(--el-text-color-primary);font-size:16px;font-weight:700}.el-descriptions__body{background-color:var(--el-fill-color-blank)}.el-descriptions__body 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var(--el-menu-base-level-padding)}.horizontal-collapse-transition .el-sub-menu__title 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.el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small .el-radio__inner{height:12px;width:12px}.el-rate{--el-rate-height:20px;--el-rate-font-size:var(--el-font-size-base);--el-rate-icon-size:18px;--el-rate-icon-margin:6px;--el-rate-void-color:var(--el-border-color-darker);--el-rate-fill-color:#f7ba2a;--el-rate-disabled-void-color:var(--el-fill-color);--el-rate-text-color:var(--el-text-color-primary);align-items:center;display:inline-flex;height:32px}.el-rate:active,.el-rate:focus{outline:none}.el-rate__item{color:var(--el-rate-void-color);cursor:pointer;display:inline-block;font-size:0;line-height:normal;position:relative;vertical-align:middle}.el-rate .el-rate__icon{display:inline-block;font-size:var(--el-rate-icon-size);margin-right:var(--el-rate-icon-margin);position:relative;transition:var(--el-transition-duration)}.el-rate .el-rate__icon.hover{transform:scale(1.15)}.el-rate .el-rate__icon .path2{left:0;position:absolute;top:0}.el-rate .el-rate__icon.is-active{color:var(--el-rate-fill-color)}.el-rate__decimal{color:var(--el-rate-fill-color);display:inline-block;overflow:hidden}.el-rate__decimal,.el-rate__decimal--box{left:0;position:absolute;top:0}.el-rate__text{color:var(--el-rate-text-color);font-size:var(--el-rate-font-size);vertical-align:middle}.el-rate--large{height:40px}.el-rate--small{height:24px}.el-rate--small .el-rate__icon{font-size:14px}.el-rate.is-disabled .el-rate__item{color:var(--el-rate-disabled-void-color);cursor:auto}.el-result{--el-result-padding:40px 30px;--el-result-icon-font-size:64px;--el-result-title-font-size:20px;--el-result-title-margin-top:20px;--el-result-subtitle-margin-top:10px;--el-result-extra-margin-top:30px;align-items:center;box-sizing:border-box;display:flex;flex-direction:column;justify-content:center;padding:var(--el-result-padding);text-align:center}.el-result__icon svg{height:var(--el-result-icon-font-size);width:var(--el-result-icon-font-size)}.el-result__title{margin-top:var(--el-result-title-margin-top)}.el-result__title p{color:var(--el-text-color-primary);font-size:var(--el-result-title-font-size);line-height:1.3;margin:0}.el-result__subtitle{margin-top:var(--el-result-subtitle-margin-top)}.el-result__subtitle p{color:var(--el-text-color-regular);font-size:var(--el-font-size-base);line-height:1.3;margin:0}.el-result__extra{margin-top:var(--el-result-extra-margin-top)}.el-result .icon-primary{--el-result-color:var(--el-color-primary);color:var(--el-result-color)}.el-result .icon-success{--el-result-color:var(--el-color-success);color:var(--el-result-color)}.el-result .icon-warning{--el-result-color:var(--el-color-warning);color:var(--el-result-color)}.el-result .icon-danger{--el-result-color:var(--el-color-danger);color:var(--el-result-color)}.el-result .icon-error{--el-result-color:var(--el-color-error);color:var(--el-result-color)}.el-result 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.el-tag__close{color:var(--el-tag-text-color);flex-shrink:0}.el-tag .el-tag__close:hover{background-color:var(--el-tag-hover-color);color:var(--el-color-white)}.el-tag.el-tag--primary{--el-tag-text-color:var(--el-color-primary)}.el-tag.el-tag--success{--el-tag-text-color:var(--el-color-success)}.el-tag.el-tag--warning{--el-tag-text-color:var(--el-color-warning)}.el-tag.el-tag--danger{--el-tag-text-color:var(--el-color-danger)}.el-tag.el-tag--error{--el-tag-text-color:var(--el-color-error)}.el-tag.el-tag--info{--el-tag-text-color:var(--el-color-info)}.el-tag .el-icon{border-radius:50%;cursor:pointer;font-size:calc(var(--el-icon-size) - 2px);height:var(--el-icon-size);width:var(--el-icon-size)}.el-tag 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0;min-width:0}.time-select .el-picker-panel__content{margin:0;max-height:200px}.time-select-item{font-size:14px;line-height:20px;padding:8px 10px}.time-select-item.disabled{color:var(--el-datepicker-border-color);cursor:not-allowed}.time-select-item:hover{background-color:var(--el-fill-color-light);cursor:pointer;font-weight:700}.time-select .time-select-item.selected:not(.disabled){color:var(--el-color-primary);font-weight:700}.el-timeline-item{padding-bottom:20px;position:relative}.el-timeline-item__wrapper{padding-left:28px;position:relative;top:-3px}.el-timeline-item__tail{border-left:2px solid var(--el-timeline-node-color);height:100%;left:4px;position:absolute}.el-timeline-item .el-timeline-item__icon{color:var(--el-color-white);font-size:var(--el-font-size-small)}.el-timeline-item__node{align-items:center;background-color:var(--el-timeline-node-color);border-color:var(--el-timeline-node-color);border-radius:50%;box-sizing:border-box;display:flex;justify-content:center;position:absolute}.el-timeline-item__node--normal{height:var(--el-timeline-node-size-normal);left:-1px;width:var(--el-timeline-node-size-normal)}.el-timeline-item__node--large{height:var(--el-timeline-node-size-large);left:-2px;width:var(--el-timeline-node-size-large)}.el-timeline-item__node.is-hollow{background:var(--el-color-white);border-style:solid;border-width:2px}.el-timeline-item__node--primary{background-color:var(--el-color-primary);border-color:var(--el-color-primary)}.el-timeline-item__node--success{background-color:var(--el-color-success);border-color:var(--el-color-success)}.el-timeline-item__node--warning{background-color:var(--el-color-warning);border-color:var(--el-color-warning)}.el-timeline-item__node--danger{background-color:var(--el-color-danger);border-color:var(--el-color-danger)}.el-timeline-item__node--info{background-color:var(--el-color-info);border-color:var(--el-color-info)}.el-timeline-item__dot{align-items:center;display:flex;justify-content:center;position:absolute}.el-timeline-item__content{color:var(--el-text-color-primary)}.el-timeline-item__timestamp{color:var(--el-text-color-secondary);font-size:var(--el-font-size-small);line-height:1}.el-timeline-item__timestamp.is-top{margin-bottom:8px;padding-top:4px}.el-timeline-item__timestamp.is-bottom{margin-top:8px}.el-timeline{--el-timeline-node-size-normal:12px;--el-timeline-node-size-large:14px;--el-timeline-node-color:var(--el-border-color-light);font-size:var(--el-font-size-base);list-style:none;margin:0}.el-timeline 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role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Human Practices<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li></ul><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-mobile px-10 flex lg:hidden"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 2x" class="cursor-pointer py-2" src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div class="grow"></div><!--[--><span class="el-avatar el-avatar--icon el-avatar--circle self-center cursor-pointer el-tooltip__trigger el-tooltip__trigger" style=""><i class="el-icon" style=""><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M160 448a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zM160 896a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32z"></path></svg><!--]--></i></span><!--teleport start--><!--teleport end--><!--]--></ul></header><div class="pt-[8rem]"><!--[--><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="cover" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/wetlab.webp 1x, https://static.igem.wiki/teams/5358/banner/wetlab.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/wetlab.webp" data-v-651baa18><div class="page-title m-10 mb-16 font-normal text-3xl p-5 rounded-lg" data-v-651baa18>Experiments</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10" data-v-651baa18><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[250px] md:mr-10" data-v-651baa18><h1 class="mt-0" data-v-651baa18>TOC</h1><ul data-v-651baa18><!--[--><li class="my-4" data-v-651baa18><a class="cursor-pointer" data-v-651baa18>What IS Endocrine Pollutant？</a><ul class="hidden pl-5 pt-2" data-v-651baa18><!--[--><!--]--></ul></li><li class="my-4" data-v-651baa18><a class="cursor-pointer" data-v-651baa18>What IS DNA Damage？</a><ul class="hidden pl-5 pt-2" data-v-651baa18><!--[--><!--]--></ul></li><li class="my-4" data-v-651baa18><a class="cursor-pointer" data-v-651baa18>Experiment</a><ul class="hidden pl-5 pt-2" data-v-651baa18><!--[--><!--[--><li class="text-[#fff] font-normal mb-2" data-v-651baa18><a class="cursor-pointer" data-v-651baa18>Strain Obtain</a></li><!--]--><!--[--><li class="text-[#fff] font-normal mb-2" data-v-651baa18><a class="cursor-pointer" data-v-651baa18>Characterization experiment</a></li><!--]--><!--]--></ul></li><!--]--></ul></aside><div class="content" data-v-651baa18><h2 id="what-is-endocrine-pollutant"><!--[-->What IS Endocrine Pollutant？<!--]--></h2><p><!--[-->Endocrine pollutants, also known as endocrine-disrupting chemicals (EDCs), are substances that can interfere with the normal functioning of the endocrine system. These chemicals mimic, block, or alter the effects of hormones in the body, potentially leading to adverse health effects. The endocrine system, which includes glands such as the thyroid, adrenal, and pituitary, is responsible for releasing hormones that regulate many vital functions, including growth, metabolism, and reproduction.<!--]--></p><h2 id="what-is-dna-damage"><!--[-->What IS DNA Damage？<!--]--></h2><p><!--[-->DNA damage refers to alterations in the chemical structure of DNA, which can affect its function and integrity. This damage can occur through various mechanisms and can lead to a range of cellular consequences if not properly repaired.<!--]--></p><h2 id="experiment"><!--[-->Experiment<!--]--></h2><h3 id="strain-obtain"><!--[--><strong><!--[-->Strain Obtain<!--]--></strong><!--]--></h3><h4 id="gene-acquisition"><!--[--><strong><!--[-->gene acquisition<!--]--></strong><!--]--></h4><p><!--[-->The recA，rbs30，hrpR，hrpS，PhrpL ，eGFP，BsFbFp，PgapA，T7RNAP，VAMER intein，PT7，LuxI and LuxR genes were synthesized by Sangon Biotech according to the standard of biobrick, and the line fragments were obtained through PCR.<!--]--></p><h5 id="pcr"><!--[--><em><!--[--><strong><!--[-->PCR<!--]--></strong><!--]--></em><!--]--></h5><p><!--[-->Material<!--]--></p><p><!--[-->Thermocycler, (0.2ml)PCR tube, pipetting gun, pipette tip, 2xPCRmix, upstream primer, downstream primer, sterile water, DNA template.<!--]--></p><p><!--[-->Protocol<!--]--></p><ul><!--[--><li><!--[-->Add to PCR tube (the total system volume: 25 μL): 12.5 μL 2×PCRmix, 9 μL of sterile water, 1.5 μl DNA template, 1 μL reverse primer, 1 μL forward primer;<!--]--></li><li><!--[-->Put the PCR tube into the thermocycler and execute the program: pre-denaturation at 94°C for 210s; followed by 30 cycles of denaturation at 94°C for 30s, anneal at the range of 52-62°C for 30s, extension at 72°C for 60s;and a final extension at 72°C for 5 min;<!--]--></li><li><!--[-->The product is stored at -10°C;<!--]--></li><!--]--></ul><h5 id="agarose-gel-electrophoresis-and-sample-recovery"><!--[--><strong><!--[-->Agarose gel electrophoresis and sample recovery<!--]--></strong><!--]--></h5><p><!--[-->Material<!--]--></p><p><!--[-->Electric hot water bath, electronic balance, electrophoresis instrument, gel imaging analysis system, pipetting gun, pipette tip, glass bottle, agarose, electrophoresis buffer, nucleic acid dye, DNA loading buffer, DNA precipitation buffer, gel buffer, elution buffer;<!--]--></p><p><!--[-->Protocol<!--]--></p><p><!--[--><strong><!--[-->Agarose gel electrophoresis<!--]--></strong><!--]--></p><ul><!--[--><li><!--[-->2% agarose solution: add 30mL of 1X TAE electrophoresis buffer into a glass bottle. then weigh 0.6g of agarose and add it into the bottle;<!--]--></li><li><!--[-->Heat the mixture(2% agarose solution) to boiling. Add 3μL of fluorescent dye to the heated liquid and mix it completely. Take out the glue box and place a mold in the box. Then pour the mixture into the mold and wait for solidification at room temperature;<!--]--></li><li><!--[-->Remove the cooled and formed agarose gel and place it in the electrophoresis instrument. Mix the DNA samples with 1/5 of DNA precipitation buffer thoroughly. Then inject the standard samples and the mixture into different holes of the gel. Turn on the electrophoresis instrument and adjust the voltage to 110V for 40 min;<!--]--></li><li><!--[-->Take out the agarose gel and open the gel imaging analysis instrument to analyze and save the electrophoresis results.<!--]--></li><!--]--></ul><p><!--[--><strong><!--[-->Sample recovery<!--]--></strong><!--]--></p><p><!--[-->The electrophoresis products were purified by gel recovery method, and the purified materials were using the DNA gel recovery kit of Axygen . The operation steps are as follows :<!--]--></p><ul><!--[--><li><!--[-->Open the water bath in advance and set the temperature to 75°C(167°F);<!--]--></li><li><!--[-->Use Agarose gel electrophoresis to verify the DNA fragment and observe in the gel imaging system. Cut the agarose gel containing the target band with a knife under the ultraviolet lamp, and record the weight of the 1.5 mL centrifuge tube in advance. After the centrifuge tube is loaded, weigh it again and calculate the gel weight. 100 mg is a gel volume equivalent to 100 μL;<!--]--></li><li><!--[-->Add 3 gel volume of gel buffer DE-A, mix well and put into 75°C water bath heating 6-8 min, mix it up and down every 2 min during water bath;<!--]--></li><li><!--[-->Add 0.5 DE-A volume of gel buffer DE-B, uniform mix it, and add 1 gel volume of isopropanol;<!--]--></li><li><!--[-->Purify the resulting products by centrifugation. Transfer the above solution to an adsorption column and place it in a 2mL centrifuge tube. Centrifuge at 12,000 rpm for 1 min, discard the waste liquid, and place the adsorption column back into the collection tube;<!--]--></li><li><!--[-->Add 500 μL buffer W1 to the adsorption column, centrifuge at 12,000 rpm for 30s, pour the waste liquid, and place the adsorption column back into the collection tube;<!--]--></li><li><!--[-->Add 700 μL buffer W2 to the adsorption column, centrifuge at 12,000 rpm for 30s, pour the waste liquid and place the adsorption column back into the collection tube, and repeat the operation once again;<!--]--></li><li><!--[-->Put the adsorption column in a new 1.5ml centrifuge tube, add 30μl Eluent in the center of the adsorption column membrane, and then centrifuge at the 12,000rpm for 1min after standing at room temperature, from which electrophoretic products were obtained;<!--]--></li><!--]--></ul><h4 id="plasmid-construction"><!--[-->Plasmid Construction<!--]--></h4><p><!--[-->The obtained gene line fragment and pUC19 empty vector are digested and ligated by enzyme ligation.<!--]--></p><h5 id="enzyme-digestion"><!--[-->Enzyme digestion<!--]--></h5><p><!--[-->Material<!--]--></p><p><!--[-->Incubator, (0.2ml)PCR tube, pipetting gun, pipette tip, restriction enzyme, NEB Buffer 3.1, target gene<!--]--></p><p><!--[-->Protocol<!--]--></p><p><!--[-->After adding the following enzyme digestion system to a 0.2 mL centrifuge tube, incubate it in an incubator at 37°C for 2 h.<!--]--></p><table><!--[--><thead><!--[--><tr><!--[--><th><!--[-->Enzyme digestion system<!--]--></th><th><!--[-->Volume<!--]--></th><!--]--></tr><!--]--></thead><tbody><!--[--><tr><!--[--><td><!--[-->Plasmid<!--]--></td><td><!--[-->17μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->NEB Buffer3.1<!--]--></td><td><!--[-->2μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->Enzyme A<!--]--></td><td><!--[-->0.5μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->Enzyme B<!--]--></td><td><!--[-->0.5μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->Total<!--]--></td><td><!--[-->20μL<!--]--></td><!--]--></tr><!--]--></tbody><!--]--></table><h5 id="enzyme-ligation"><!--[-->Enzyme Ligation<!--]--></h5><p><!--[-->Material<!--]--></p><p><!--[-->Incubator, (0.2ml) PCR tubes, pipetting gun, pipette tip, T4 DNA ligase Buffer 10x, Nuclease-Free Water, T4 DNA ligase, vector fragment, target fragment;<!--]--></p><p><!--[-->Protocol<!--]--></p><p><!--[-->After adding the following ligation system to a 0.2mL centrifuge tube, incubate it in an incubator at 37°C for 24 h.<!--]--></p><table><!--[--><thead><!--[--><tr><!--[--><th><!--[-->Connection system<!--]--></th><th><!--[-->Volume<!--]--></th><!--]--></tr><!--]--></thead><tbody><!--[--><tr><!--[--><td><!--[-->Vector fragment<!--]--></td><td><!--[-->3μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->Target fragment<!--]--></td><td><!--[-->12μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->T4 DNA ligase Buffer<!--]--></td><td><!--[-->2.5μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->Nuclease-Free water<!--]--></td><td><!--[-->6.5μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->T4 DNA ligase<!--]--></td><td><!--[-->1μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->Total<!--]--></td><td><!--[-->25μL<!--]--></td><!--]--></tr><!--]--></tbody><!--]--></table><h5 id="transformation-and-selection"><!--[-->Transformation and Selection<!--]--></h5><p><!--[-->Transfer 5μL of the ligation product into 50μL of BL21 competent cells. After incubation, plate 100μL of the transformed cells onto LB agar plates containing 100μg/mL ampicillin resistance. Invert the plates and incubate overnight at 37°C. Pick single colonies and inoculate them into 30mL of antibiotic-free LB broth. Add 30μL of ampicillin and incubate at 37°C with shaking at 180r/min for 12 h. Preserve the cultures in glycerol at -10°C;<!--]--></p><h5 id="plasmid-transformation"><!--[-->Plasmid transformation<!--]--></h5><p><!--[-->Material<!--]--></p><p><!--[-->Vertical clean bench, shaker, water bath, centrifuge, pipetting gun, pipette tip, solid culture dish, alcohol lamp, applicator, ampicillin, LB-free liquid medium, competent cells;<!--]--></p><p><!--[-->Protocol<!--]--></p><ul><!--[--><li><!--[-->Fill the foam box with about 2/3 of the volume of ice, and take100μL of prepared competent cells. turn on the ultra-clean table UV lamp in advance for 15min for sterilization. After sterilization, light up the alcohol lamp in the ultra-clean table, and transfer 50μL competent cells into a new aseptic centrifuge tube with a pipette beside the alcohol lamp, and add 5μL plasmid solution into it.<!--]--></li><li><!--[-->place the centrifuge tube containing the plasmid solution in the ice box for 30min, turn on the water bath in advance and set the temperature at 42°C, heat the centrifuge tube after the ice bath in the water bath at 42°C for 45s, and immediately remove the centrifuge tube and place it in the ice box for 2min to complete the transformation process;<!--]--></li><li><!--[-->Take out the packaged liquid medium without anti-LB, and add 500μL liquid medium into the centrifuge tube after heating. Incubate the centrifuge tube in a shaking table at 37°C 180r/min for 1h;<!--]--></li><li><!--[-->After removing the centrifuge tube from the shaker, centrifuge it at 7000rpm for 30 s. Carefully aspirate and discard the supernatant (400μL) next to an alcohol lamp. Resuspend the remaining bacterial pellet using a pipetting gun, and based on plasmid resistance, select solid medium containing ampicillin resistance. Using a pipetting gun next to an alcohol lamp, add 100μL of the resuspended bacterial culture onto the surface of the solid medium. Spread evenly using a spreader. Cover the petri dish and invert it, then place it in a 37°C incubator for 12 h;<!--]--></li><li><!--[-->Add 30μL of ampicillin to 30mL of antibiotic-free LB liquid medium. Using a pipette tip, pick a single colony and inoculate it into the aforementioned liquid medium. Incubate at 37°C with shaking at 180r/min for 12 h;<!--]--></li><!--]--></ul><h3 id="characterization-experiment"><!--[-->Characterization experiment<!--]--></h3><h4 id="preparation"><!--[-->Preparation<!--]--></h4><p><!--[-->Transfer The bacteria solution cultured overnight to 30mL fresh non-resistant LB medium, with adding 30μL ampicillin, and culture in the shaking table at 37°C and 180r/min until the OD600 value of the bacteria solution is between 0.3 and 0.4;<!--]--></p><h4 id="experimental-characterization-of-the-anaerobic-environment"><!--[-->Experimental characterization of the anaerobic environment<!--]--></h4><p><!--[-->Method 1：The bacterial solution was added to DNA damaging agent, dropped into anaerobic row and incubated for 2h in a 37 C incubator. Measure OD600 and fluorescence intensity of bacterial solution by microplate reader;<!--]--></p><p><!--[-->Method 2：The bacterial solution was sealed using paraffin oil and DNA damaging agents were added under the paraffin oil page and incubated for 2h in a 37 C incubator. Measure OD600 and fluorescence intensity of bacterial solution by microplate reader;<!--]--></p><table><!--[--><thead><!--[--><tr><!--[--><th><!--[-->DNA damaging reagent<!--]--></th><th><!--[-->Concentration gradient<!--]--></th><th><!--[--><!--]--></th><th><!--[--><!--]--></th><th><!--[--><!--]--></th><th><!--[--><!--]--></th><th><!--[--><!--]--></th><th><!--[--><!--]--></th><!--]--></tr><!--]--></thead><tbody><!--[--><tr><!--[--><td><!--[-->NA<!--]--></td><td><!--[-->0μL<!--]--></td><td><!--[-->1μL<!--]--></td><td><!--[-->5μL<!--]--></td><td><!--[-->10μL<!--]--></td><td><!--[-->15μL<!--]--></td><td><!--[-->20μL<!--]--></td><td><!--[-->25μL<!--]--></td><!--]--></tr><!--]--></tbody><!--]--></table><h4 id="data-measurement"><!--[-->Data measurement<!--]--></h4><h5 id="measure-the-od600"><!--[-->Measure the OD600<!--]--></h5><p><!--[-->Transfer 200μL of the bacteria solution to a 96-hole black microplate and place it in a microplate reader. Set the wavelength to 600 and measure the absorbance reading.<!--]--></p><h5 id="measure-the-fluorescence-intensity"><!--[-->Measure the Fluorescence Intensity<!--]--></h5><p><!--[-->Transfer 200μL of the bacteria solution to a 96-hole black microplate and place it in a microplate reader. Set the excitation wavelength to 488 nm, emission wavelength to 520 nm, and gain to 75. Measure the fluorescence intensity.<!--]--></p><p><!--[-->Each group of data was measured three times and averaged.<!--]--></p></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a aria-current="page" href="/bit/experiment" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/experiment/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-yAJtGgOdxJ":11},null,"/experiment",1727868995279]</script>
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role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Human Practices<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li></ul><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-mobile px-10 flex lg:hidden"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 2x" class="cursor-pointer py-2" src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div class="grow"></div><!--[--><span class="el-avatar el-avatar--icon el-avatar--circle self-center cursor-pointer el-tooltip__trigger el-tooltip__trigger" style=""><i class="el-icon" style=""><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M160 448a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zM160 896a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32z"></path></svg><!--]--></i></span><!--teleport start--><!--teleport end--><!--]--></ul></header><div class="pt-[8rem]"><!--[--><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="cover" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/wetlab.webp 1x, https://static.igem.wiki/teams/5358/banner/wetlab.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/wetlab.webp" data-v-651baa18><div class="page-title m-10 mb-16 font-normal text-3xl p-5 rounded-lg" data-v-651baa18>Experiments</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10" data-v-651baa18><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[250px] md:mr-10" data-v-651baa18><h1 class="mt-0" data-v-651baa18>TOC</h1><ul data-v-651baa18><!--[--><li class="my-4" data-v-651baa18><a class="cursor-pointer" data-v-651baa18>What IS Endocrine Pollutant？</a><ul class="hidden pl-5 pt-2" data-v-651baa18><!--[--><!--]--></ul></li><li class="my-4" data-v-651baa18><a class="cursor-pointer" data-v-651baa18>What IS DNA Damage？</a><ul class="hidden pl-5 pt-2" data-v-651baa18><!--[--><!--]--></ul></li><li class="my-4" data-v-651baa18><a class="cursor-pointer" data-v-651baa18>Experiment</a><ul class="hidden pl-5 pt-2" data-v-651baa18><!--[--><!--[--><li class="text-[#fff] font-normal mb-2" data-v-651baa18><a class="cursor-pointer" data-v-651baa18>Strain Obtain</a></li><!--]--><!--[--><li class="text-[#fff] font-normal mb-2" data-v-651baa18><a class="cursor-pointer" data-v-651baa18>Characterization experiment</a></li><!--]--><!--]--></ul></li><!--]--></ul></aside><div class="content" data-v-651baa18><h2 id="what-is-endocrine-pollutant"><!--[-->What IS Endocrine Pollutant？<!--]--></h2><p><!--[-->Endocrine pollutants, also known as endocrine-disrupting chemicals (EDCs), are substances that can interfere with the normal functioning of the endocrine system. These chemicals mimic, block, or alter the effects of hormones in the body, potentially leading to adverse health effects. The endocrine system, which includes glands such as the thyroid, adrenal, and pituitary, is responsible for releasing hormones that regulate many vital functions, including growth, metabolism, and reproduction.<!--]--></p><h2 id="what-is-dna-damage"><!--[-->What IS DNA Damage？<!--]--></h2><p><!--[-->DNA damage refers to alterations in the chemical structure of DNA, which can affect its function and integrity. This damage can occur through various mechanisms and can lead to a range of cellular consequences if not properly repaired.<!--]--></p><h2 id="experiment"><!--[-->Experiment<!--]--></h2><h3 id="strain-obtain"><!--[--><strong><!--[-->Strain Obtain<!--]--></strong><!--]--></h3><h4 id="gene-acquisition"><!--[--><strong><!--[-->gene acquisition<!--]--></strong><!--]--></h4><p><!--[-->The recA，rbs30，hrpR，hrpS，PhrpL ，eGFP，BsFbFp，PgapA，T7RNAP，VAMER intein，PT7，LuxI and LuxR genes were synthesized by Sangon Biotech according to the standard of biobrick, and the line fragments were obtained through PCR.<!--]--></p><h5 id="pcr"><!--[--><em><!--[--><strong><!--[-->PCR<!--]--></strong><!--]--></em><!--]--></h5><p><!--[-->Material<!--]--></p><p><!--[-->Thermocycler, (0.2ml)PCR tube, pipetting gun, pipette tip, 2xPCRmix, upstream primer, downstream primer, sterile water, DNA template.<!--]--></p><p><!--[-->Protocol<!--]--></p><ul><!--[--><li><!--[-->Add to PCR tube (the total system volume: 25 μL): 12.5 μL 2×PCRmix, 9 μL of sterile water, 1.5 μl DNA template, 1 μL reverse primer, 1 μL forward primer;<!--]--></li><li><!--[-->Put the PCR tube into the thermocycler and execute the program: pre-denaturation at 94°C for 210s; followed by 30 cycles of denaturation at 94°C for 30s, anneal at the range of 52-62°C for 30s, extension at 72°C for 60s;and a final extension at 72°C for 5 min;<!--]--></li><li><!--[-->The product is stored at -10°C;<!--]--></li><!--]--></ul><h5 id="agarose-gel-electrophoresis-and-sample-recovery"><!--[--><strong><!--[-->Agarose gel electrophoresis and sample recovery<!--]--></strong><!--]--></h5><p><!--[-->Material<!--]--></p><p><!--[-->Electric hot water bath, electronic balance, electrophoresis instrument, gel imaging analysis system, pipetting gun, pipette tip, glass bottle, agarose, electrophoresis buffer, nucleic acid dye, DNA loading buffer, DNA precipitation buffer, gel buffer, elution buffer;<!--]--></p><p><!--[-->Protocol<!--]--></p><p><!--[--><strong><!--[-->Agarose gel electrophoresis<!--]--></strong><!--]--></p><ul><!--[--><li><!--[-->2% agarose solution: add 30mL of 1X TAE electrophoresis buffer into a glass bottle. then weigh 0.6g of agarose and add it into the bottle;<!--]--></li><li><!--[-->Heat the mixture(2% agarose solution) to boiling. Add 3μL of fluorescent dye to the heated liquid and mix it completely. Take out the glue box and place a mold in the box. Then pour the mixture into the mold and wait for solidification at room temperature;<!--]--></li><li><!--[-->Remove the cooled and formed agarose gel and place it in the electrophoresis instrument. Mix the DNA samples with 1/5 of DNA precipitation buffer thoroughly. Then inject the standard samples and the mixture into different holes of the gel. Turn on the electrophoresis instrument and adjust the voltage to 110V for 40 min;<!--]--></li><li><!--[-->Take out the agarose gel and open the gel imaging analysis instrument to analyze and save the electrophoresis results.<!--]--></li><!--]--></ul><p><!--[--><strong><!--[-->Sample recovery<!--]--></strong><!--]--></p><p><!--[-->The electrophoresis products were purified by gel recovery method, and the purified materials were using the DNA gel recovery kit of Axygen . The operation steps are as follows :<!--]--></p><ul><!--[--><li><!--[-->Open the water bath in advance and set the temperature to 75°C(167°F);<!--]--></li><li><!--[-->Use Agarose gel electrophoresis to verify the DNA fragment and observe in the gel imaging system. Cut the agarose gel containing the target band with a knife under the ultraviolet lamp, and record the weight of the 1.5 mL centrifuge tube in advance. After the centrifuge tube is loaded, weigh it again and calculate the gel weight. 100 mg is a gel volume equivalent to 100 μL;<!--]--></li><li><!--[-->Add 3 gel volume of gel buffer DE-A, mix well and put into 75°C water bath heating 6-8 min, mix it up and down every 2 min during water bath;<!--]--></li><li><!--[-->Add 0.5 DE-A volume of gel buffer DE-B, uniform mix it, and add 1 gel volume of isopropanol;<!--]--></li><li><!--[-->Purify the resulting products by centrifugation. Transfer the above solution to an adsorption column and place it in a 2mL centrifuge tube. Centrifuge at 12,000 rpm for 1 min, discard the waste liquid, and place the adsorption column back into the collection tube;<!--]--></li><li><!--[-->Add 500 μL buffer W1 to the adsorption column, centrifuge at 12,000 rpm for 30s, pour the waste liquid, and place the adsorption column back into the collection tube;<!--]--></li><li><!--[-->Add 700 μL buffer W2 to the adsorption column, centrifuge at 12,000 rpm for 30s, pour the waste liquid and place the adsorption column back into the collection tube, and repeat the operation once again;<!--]--></li><li><!--[-->Put the adsorption column in a new 1.5ml centrifuge tube, add 30μl Eluent in the center of the adsorption column membrane, and then centrifuge at the 12,000rpm for 1min after standing at room temperature, from which electrophoretic products were obtained;<!--]--></li><!--]--></ul><h4 id="plasmid-construction"><!--[-->Plasmid Construction<!--]--></h4><p><!--[-->The obtained gene line fragment and pUC19 empty vector are digested and ligated by enzyme ligation.<!--]--></p><h5 id="enzyme-digestion"><!--[-->Enzyme digestion<!--]--></h5><p><!--[-->Material<!--]--></p><p><!--[-->Incubator, (0.2ml)PCR tube, pipetting gun, pipette tip, restriction enzyme, NEB Buffer 3.1, target gene<!--]--></p><p><!--[-->Protocol<!--]--></p><p><!--[-->After adding the following enzyme digestion system to a 0.2 mL centrifuge tube, incubate it in an incubator at 37°C for 2 h.<!--]--></p><table><!--[--><thead><!--[--><tr><!--[--><th><!--[-->Enzyme digestion system<!--]--></th><th><!--[-->Volume<!--]--></th><!--]--></tr><!--]--></thead><tbody><!--[--><tr><!--[--><td><!--[-->Plasmid<!--]--></td><td><!--[-->17μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->NEB Buffer3.1<!--]--></td><td><!--[-->2μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->Enzyme A<!--]--></td><td><!--[-->0.5μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->Enzyme B<!--]--></td><td><!--[-->0.5μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->Total<!--]--></td><td><!--[-->20μL<!--]--></td><!--]--></tr><!--]--></tbody><!--]--></table><h5 id="enzyme-ligation"><!--[-->Enzyme Ligation<!--]--></h5><p><!--[-->Material<!--]--></p><p><!--[-->Incubator, (0.2ml) PCR tubes, pipetting gun, pipette tip, T4 DNA ligase Buffer 10x, Nuclease-Free Water, T4 DNA ligase, vector fragment, target fragment;<!--]--></p><p><!--[-->Protocol<!--]--></p><p><!--[-->After adding the following ligation system to a 0.2mL centrifuge tube, incubate it in an incubator at 37°C for 24 h.<!--]--></p><table><!--[--><thead><!--[--><tr><!--[--><th><!--[-->Connection system<!--]--></th><th><!--[-->Volume<!--]--></th><!--]--></tr><!--]--></thead><tbody><!--[--><tr><!--[--><td><!--[-->Vector fragment<!--]--></td><td><!--[-->3μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->Target fragment<!--]--></td><td><!--[-->12μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->T4 DNA ligase Buffer<!--]--></td><td><!--[-->2.5μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->Nuclease-Free water<!--]--></td><td><!--[-->6.5μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->T4 DNA ligase<!--]--></td><td><!--[-->1μL<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->Total<!--]--></td><td><!--[-->25μL<!--]--></td><!--]--></tr><!--]--></tbody><!--]--></table><h5 id="transformation-and-selection"><!--[-->Transformation and Selection<!--]--></h5><p><!--[-->Transfer 5μL of the ligation product into 50μL of BL21 competent cells. After incubation, plate 100μL of the transformed cells onto LB agar plates containing 100μg/mL ampicillin resistance. Invert the plates and incubate overnight at 37°C. Pick single colonies and inoculate them into 30mL of antibiotic-free LB broth. Add 30μL of ampicillin and incubate at 37°C with shaking at 180r/min for 12 h. Preserve the cultures in glycerol at -10°C;<!--]--></p><h5 id="plasmid-transformation"><!--[-->Plasmid transformation<!--]--></h5><p><!--[-->Material<!--]--></p><p><!--[-->Vertical clean bench, shaker, water bath, centrifuge, pipetting gun, pipette tip, solid culture dish, alcohol lamp, applicator, ampicillin, LB-free liquid medium, competent cells;<!--]--></p><p><!--[-->Protocol<!--]--></p><ul><!--[--><li><!--[-->Fill the foam box with about 2/3 of the volume of ice, and take100μL of prepared competent cells. turn on the ultra-clean table UV lamp in advance for 15min for sterilization. After sterilization, light up the alcohol lamp in the ultra-clean table, and transfer 50μL competent cells into a new aseptic centrifuge tube with a pipette beside the alcohol lamp, and add 5μL plasmid solution into it.<!--]--></li><li><!--[-->place the centrifuge tube containing the plasmid solution in the ice box for 30min, turn on the water bath in advance and set the temperature at 42°C, heat the centrifuge tube after the ice bath in the water bath at 42°C for 45s, and immediately remove the centrifuge tube and place it in the ice box for 2min to complete the transformation process;<!--]--></li><li><!--[-->Take out the packaged liquid medium without anti-LB, and add 500μL liquid medium into the centrifuge tube after heating. Incubate the centrifuge tube in a shaking table at 37°C 180r/min for 1h;<!--]--></li><li><!--[-->After removing the centrifuge tube from the shaker, centrifuge it at 7000rpm for 30 s. Carefully aspirate and discard the supernatant (400μL) next to an alcohol lamp. Resuspend the remaining bacterial pellet using a pipetting gun, and based on plasmid resistance, select solid medium containing ampicillin resistance. Using a pipetting gun next to an alcohol lamp, add 100μL of the resuspended bacterial culture onto the surface of the solid medium. Spread evenly using a spreader. Cover the petri dish and invert it, then place it in a 37°C incubator for 12 h;<!--]--></li><li><!--[-->Add 30μL of ampicillin to 30mL of antibiotic-free LB liquid medium. Using a pipette tip, pick a single colony and inoculate it into the aforementioned liquid medium. Incubate at 37°C with shaking at 180r/min for 12 h;<!--]--></li><!--]--></ul><h3 id="characterization-experiment"><!--[-->Characterization experiment<!--]--></h3><h4 id="preparation"><!--[-->Preparation<!--]--></h4><p><!--[-->Transfer The bacteria solution cultured overnight to 30mL fresh non-resistant LB medium, with adding 30μL ampicillin, and culture in the shaking table at 37°C and 180r/min until the OD600 value of the bacteria solution is between 0.3 and 0.4;<!--]--></p><h4 id="experimental-characterization-of-the-anaerobic-environment"><!--[-->Experimental characterization of the anaerobic environment<!--]--></h4><p><!--[-->Method 1：The bacterial solution was added to DNA damaging agent, dropped into anaerobic row and incubated for 2h in a 37 C incubator. Measure OD600 and fluorescence intensity of bacterial solution by microplate reader;<!--]--></p><p><!--[-->Method 2：The bacterial solution was sealed using paraffin oil and DNA damaging agents were added under the paraffin oil page and incubated for 2h in a 37 C incubator. Measure OD600 and fluorescence intensity of bacterial solution by microplate reader;<!--]--></p><table><!--[--><thead><!--[--><tr><!--[--><th><!--[-->DNA damaging reagent<!--]--></th><th><!--[-->Concentration gradient<!--]--></th><th><!--[--><!--]--></th><!--]--></tr><!--]--></thead><tbody><!--[--><tr><!--[--><td><!--[-->NA<!--]--></td><td><!--[-->0μL<!--]--></td><td><!--[-->1μL 5μL 10μL 15μL 20μL 25μL<!--]--></td><!--]--></tr><!--]--></tbody><!--]--></table><h4 id="data-measurement"><!--[-->Data measurement<!--]--></h4><h5 id="measure-the-od600"><!--[-->Measure the OD600<!--]--></h5><p><!--[-->Transfer 200μL of the bacteria solution to a 96-hole black microplate and place it in a microplate reader. Set the wavelength to 600 and measure the absorbance reading.<!--]--></p><h5 id="measure-the-fluorescence-intensity"><!--[-->Measure the Fluorescence Intensity<!--]--></h5><p><!--[-->Transfer 200μL of the bacteria solution to a 96-hole black microplate and place it in a microplate reader. Set the excitation wavelength to 488 nm, emission wavelength to 520 nm, and gain to 75. Measure the fluorescence intensity.<!--]--></p><p><!--[-->Each group of data was measured three times and averaged.<!--]--></p></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a aria-current="page" href="/bit/experiment" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/experiment/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-yAJtGgOdxJ":11},null,"/experiment",1727871092318]</script>
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diff --git a/.output/public/hardware/_payload.json b/.output/public/hardware/_payload.json
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--- a/.output/public/hardware/_payload.json
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@@ -1 +1 @@
-[{"data":1,"prerenderedAt":767},["Reactive",2],{"content-query-1TDeI6fddl":3},{"_path":4,"_dir":5,"_draft":6,"_partial":6,"_locale":5,"title":7,"description":8,"body":9,"_type":761,"_id":762,"_source":763,"_file":764,"_stem":765,"_extension":766},"/drylab-hardware","",false,"Hardware","This year, the core objective of the BIT team is to develop a initiative detection system for EDCs in aquatic environments based on E.coli biosensors. Traditional detection methods, such as chromatography and immunoassays, require cumbersome procedures like sampling, pre-treatment, and laboratory examination, in addition to expensive laboratory equipment (e.g., chromatographs, microplate reader) and professional experimental techniques. These methods are time-consuming and costly.",{"type":10,"children":11,"toc":738},"root",[12,28,40,52,60,71,78,90,102,114,119,125,132,141,148,157,197,223,234,239,251,302,309,315,320,338,347,353,358,365,374,379,385,390,400,410,420,437,442,452,461,466,473,482,487,494,503,510,519,525,532,558,565,570,575,591,600,606,611,618,627,633,638,644,649,655,660,666,671,677,682,688,693,698,703,717],{"type":13,"tag":14,"props":15,"children":16},"element","p",{},[17,20,26],{"type":18,"value":19},"text","This year, the core objective of the BIT team is to develop ",{"type":13,"tag":21,"props":22,"children":23},"strong",{},[24],{"type":18,"value":25},"a initiative detection system for EDCs in aquatic environments based on E.coli biosensors.",{"type":18,"value":27}," Traditional detection methods, such as chromatography and immunoassays, require cumbersome procedures like sampling, pre-treatment, and laboratory examination, in addition to expensive laboratory equipment (e.g., chromatographs, microplate reader) and professional experimental techniques. These methods are time-consuming and costly.",{"type":13,"tag":14,"props":29,"children":30},{},[31,33,38],{"type":18,"value":32},"As EDCs continue to threaten aquatic environments and the quality of drinking water for residents, it is crucial to achieve portable detection and rapid response to EDCs. To meet the requirement, our goal is to develop a low costing, highly integrated, compact, portable, and user-friendly hardware platform that can be combined with smartphone software to automate the cultivation of engineering bacteria and the analysis of fluorescence ",{"type":13,"tag":21,"props":34,"children":35},{},[36],{"type":18,"value":37},"detection",{"type":18,"value":39},".",{"type":13,"tag":14,"props":41,"children":42},{},[43,45,50],{"type":18,"value":44},"To ensure that the hardware device truly meets the needs of system construction, we have adopted a typical engineering and project design approach, namely: ",{"type":13,"tag":21,"props":46,"children":47},{},[48],{"type":18,"value":49},"demand analysis, scheme proposal, feasibility verification, feedback , system improvement, and goal achievement.",{"type":18,"value":51}," This process is an iterative one, and after several cycles, it ultimately meets all the needs and objectives.",{"type":13,"tag":14,"props":53,"children":54},{},[55],{"type":13,"tag":56,"props":57,"children":59},"img",{"alt":5,"src":58},"https://static.igem.wiki/teams/5358/hardware/1.png",[],{"type":13,"tag":14,"props":61,"children":62},{},[63],{"type":13,"tag":64,"props":65,"children":68},"span",{"className":66},[67],"fig-title",[69],{"type":18,"value":70},"Figure 1 Hardware Design Flowchart",{"type":13,"tag":72,"props":73,"children":75},"h2",{"id":74},"demand-analysis",[76],{"type":18,"value":77},"Demand Analysis",{"type":13,"tag":14,"props":79,"children":80},{},[81,83,88],{"type":18,"value":82},"To ensure the stability and accuracy of ",{"type":13,"tag":21,"props":84,"children":85},{},[86],{"type":18,"value":87},"fluorescence detection",{"type":18,"value":89},", it is necessary to standardize the detection process and use high sensitive detection units.",{"type":13,"tag":14,"props":91,"children":92},{},[93,95,100],{"type":18,"value":94},"Standardization of the detection process requires a continuous and stable chemical and physical environment during the resuscitation of engineering bacteria and fluorescence detection. The standardization of the chemical environment is mainly ensured by the quantified operation of experimental steps (including control of sample volume and the number of engineering bacteria, control of the chemical composition and concentration of the culture medium), and the standardization of the physical environment is mainly ensured by the stability of light source, temperature, and humidity. Among them, light exposure problem has been solved by the sealed and light-proof nature of the device, and the humidity in the sealed environment is mainly determined by temperature. The standardization of the detection process mainly aims to achieve ",{"type":13,"tag":21,"props":96,"children":97},{},[98],{"type":18,"value":99},"constant temperature control",{"type":18,"value":101}," of the hardware device.",{"type":13,"tag":14,"props":103,"children":104},{},[105,107,112],{"type":18,"value":106},"Since the concentration of ",{"type":13,"tag":21,"props":108,"children":109},{},[110],{"type":18,"value":111},"EDCs",{"type":18,"value":113}," in the detection samples is very low，which also refer to the low intensity of fluorescence, coupled with the portability requirements of the equipment, the detection method required must be high sensitive and can be integrated into small devices. Common chemical detection methods cannot meet the requirements, so adopting a more sensitive, portable, and efficient detection method is crucial for the realization of our POCT system.",{"type":13,"tag":14,"props":115,"children":116},{},[117],{"type":18,"value":118},"In order to enable smartphone software to automate the control of hardware devices and visualize the detection results, the construction of hardware devices needs to consider the transmission of data between software and hardware.",{"type":13,"tag":72,"props":120,"children":122},{"id":121},"scheme-proposal",[123],{"type":18,"value":124},"Scheme Proposal",{"type":13,"tag":14,"props":126,"children":127},{},[128],{"type":13,"tag":56,"props":129,"children":131},{"alt":5,"src":130},"https://static.igem.wiki/teams/5358/hardware/2.png",[],{"type":13,"tag":14,"props":133,"children":134},{},[135],{"type":13,"tag":64,"props":136,"children":138},{"className":137},[67],[139],{"type":18,"value":140},"Figure 2 First Edition Design Scheme",{"type":13,"tag":14,"props":142,"children":143},{},[144],{"type":13,"tag":56,"props":145,"children":147},{"alt":5,"src":146},"https://static.igem.wiki/teams/5358/hardware/3.png",[],{"type":13,"tag":14,"props":149,"children":150},{},[151],{"type":13,"tag":64,"props":152,"children":154},{"className":153},[67],[155],{"type":18,"value":156},"Figure 3 Circuit Diagram",{"type":13,"tag":14,"props":158,"children":159},{},[160,162,167,169,174,176,181,183,188,190,195],{"type":18,"value":161},"In the construction of the ",{"type":13,"tag":21,"props":163,"children":164},{},[165],{"type":18,"value":166},"temperature control module",{"type":18,"value":168},", we employed an NTC thermistor as the ",{"type":13,"tag":21,"props":170,"children":171},{},[172],{"type":18,"value":173},"temperature sensor",{"type":18,"value":175},", utilizing heating FILM and fan as the heat and cold sources respectively. To achieve ",{"type":13,"tag":21,"props":177,"children":178},{},[179],{"type":18,"value":180},"PID regulation",{"type":18,"value":182}," for ",{"type":13,"tag":21,"props":184,"children":185},{},[186],{"type":18,"value":187},"temperature sensing and control",{"type":18,"value":189},", we used the PWM wave output from the ",{"type":13,"tag":21,"props":191,"children":192},{},[193],{"type":18,"value":194},"TB6612FNG motor drive",{"type":18,"value":196}," to adjust the power of the fan and heating FILM, thereby maintaining dynamic temperature stability.",{"type":13,"tag":14,"props":198,"children":199},{},[200,202,207,209,214,216,221],{"type":18,"value":201},"For the selection of the detection scheme, we comprehensively considered the detection accuracy and the portability of the device, ultimately choosing the ",{"type":13,"tag":21,"props":203,"children":204},{},[205],{"type":18,"value":206},"fluorescence detection module",{"type":18,"value":208},". We initially selected the AS7341 as ",{"type":13,"tag":21,"props":210,"children":211},{},[212],{"type":18,"value":213},"fluorescence sensor",{"type":18,"value":215}," ",{"type":13,"tag":64,"props":217,"children":218},{},[219],{"type":18,"value":220},"1",{"type":18,"value":222},". Concurrently, to ensure consistent fluorescence generation, we redesigned and optimized the optical path.",{"type":13,"tag":14,"props":224,"children":225},{},[226,228,233],{"type":18,"value":227},"After the marked fluorescence is excited by a blue LED, the fluorescence sensor collect and record real-time fluorescence signals, which can be visualized on the mobile software via a ",{"type":13,"tag":21,"props":229,"children":230},{},[231],{"type":18,"value":232},"communication module",{"type":18,"value":39},{"type":13,"tag":14,"props":235,"children":236},{},[237],{"type":18,"value":238},"For the communication module, we used the HC-05 Bluetooth module to connect the hardware system with the mobile software, enabling real-time monitoring of the hardware device's operating status and feedback of the detection results.",{"type":13,"tag":14,"props":240,"children":241},{},[242,244,249],{"type":18,"value":243},"In the initial version of the scheme, we used an STM32 chip as the ",{"type":13,"tag":21,"props":245,"children":246},{},[247],{"type":18,"value":248},"microcontroller Unit(MCU)",{"type":18,"value":250},", connecting temperature control module, fluorescence detection module, and Bluetooth communication module, ensuring the normal operation of the device through programming instructions.",{"type":13,"tag":252,"props":253,"children":255},"mdc-wrap",{":class":254},"table-fig",[256],{"type":13,"tag":257,"props":258,"children":259},"table",{},[260,284],{"type":13,"tag":261,"props":262,"children":263},"thead",{},[264],{"type":13,"tag":265,"props":266,"children":267},"tr",{},[268,277],{"type":13,"tag":269,"props":270,"children":272},"th",{"align":271},"center",[273],{"type":13,"tag":56,"props":274,"children":276},{"alt":5,"src":275},"https://static.igem.wiki/teams/5358/hardware/4.png",[],{"type":13,"tag":269,"props":278,"children":279},{"align":271},[280],{"type":13,"tag":56,"props":281,"children":283},{"alt":5,"src":282},"https://static.igem.wiki/teams/5358/hardware/5.png",[],{"type":13,"tag":285,"props":286,"children":287},"tbody",{},[288],{"type":13,"tag":265,"props":289,"children":290},{},[291,297],{"type":13,"tag":292,"props":293,"children":294},"td",{"align":271},[295],{"type":18,"value":296},"Figure 5:  First Edition Implementation Scheme",{"type":13,"tag":292,"props":298,"children":299},{"align":271},[300],{"type":18,"value":301},"Figure 6: First Edition Equipment Model Diagram",{"type":13,"tag":303,"props":304,"children":306},"h3",{"id":305},"feasibility-verification",[307],{"type":18,"value":308},"Feasibility Verification",{"type":13,"tag":303,"props":310,"children":312},{"id":311},"temperature-stability-verification",[313],{"type":18,"value":314},"Temperature Stability Verification",{"type":13,"tag":14,"props":316,"children":317},{},[318],{"type":18,"value":319},"To verify the constant temperature effect of the temperature control system, we designed a temperature stability test experiment. When the temperature first reached 37°C, the temperature was recorded every 10 seconds for an additional 60 seconds. From the results shown in Figure 6, it can be concluded that the absolute difference between the average temperature and the set temperature was no greater than 1.1°C, and the temperature curve consistently fluctuated around 37°C. This indicates that the temperature control system can achieve dynamic temperature regulation, capable of maintaining the temperature close to 37°C. However, this temperature control method generates relatively large temperature fluctuations.",{"type":13,"tag":14,"props":321,"children":322},{},[323],{"type":13,"tag":64,"props":324,"children":328},{"className":325},[326,327],"flex","gap-4",[329,333,334],{"type":13,"tag":56,"props":330,"children":332},{"alt":5,"src":331},"https://static.igem.wiki/teams/5358/hardware/6.png",[],{"type":18,"value":215},{"type":13,"tag":56,"props":335,"children":337},{"alt":5,"src":336},"https://static.igem.wiki/teams/5358/hardware/7.png",[],{"type":13,"tag":14,"props":339,"children":340},{},[341],{"type":13,"tag":64,"props":342,"children":344},{"className":343},[67],[345],{"type":18,"value":346},"Figure 7 Measurement Results of  Initial Scheme's Temperature Stability",{"type":13,"tag":303,"props":348,"children":350},{"id":349},"gradient-fluoresce-in-sodium-fluorescence-detection-verification",[351],{"type":18,"value":352},"Gradient Fluoresce in Sodium Fluorescence Detection Verification",{"type":13,"tag":14,"props":354,"children":355},{},[356],{"type":18,"value":357},"The AS7341 fluorescence sensor, by integrating filters of different wavelength bands, achieves the function of detecting light intensity (Lux) at specific wavelengths, which is equivalent to detecting the corresponding fluorescence intensity. To verify whether this sensor can correctly reflect the signal from the sample, we designed an experiment to explore the relationship between the concentration gradient of fluoresce in sodium and Lux. We prepared a series of fluoresce in sodium solutions with different gradients in a 2mL centrifuge tube for signal collection using the AS734. The results shown in Figure 8 indicate that the measured Lux values have a good linear correlation with the concentration gradient of fluoresce in sodium, demonstrating that our fluorescence detection module can accurately reflect the concentration of substances and is capable of conducting fluorescence detection.",{"type":13,"tag":14,"props":359,"children":360},{},[361],{"type":13,"tag":56,"props":362,"children":364},{"alt":5,"src":363},"https://static.igem.wiki/teams/5358/hardware/8.png",[],{"type":13,"tag":14,"props":366,"children":367},{},[368],{"type":13,"tag":64,"props":369,"children":371},{"className":370},[67],[372],{"type":18,"value":373},"Figure 8 Initial Scheme's Fluorescence Verification Fitting Curve",{"type":13,"tag":14,"props":375,"children":376},{},[377],{"type":18,"value":378},"The above feasibility verification demonstrates that the temperature control module and fluorescence detection module designed in the first version of the scheme can meet the design requirements and are feasible.",{"type":13,"tag":72,"props":380,"children":382},{"id":381},"feedback",[383],{"type":18,"value":384},"Feedback",{"type":13,"tag":14,"props":386,"children":387},{},[388],{"type":18,"value":389},"We engaged in in-depth discussions with the biology team, design team, and professional instructors regarding our hardware design, obtaining extremely valuable feedback. This feedback is instrumental in guiding our further improvements.",{"type":13,"tag":14,"props":391,"children":392},{},[393,398],{"type":13,"tag":21,"props":394,"children":395},{},[396],{"type":18,"value":397},"1.MCU:",{"type":18,"value":399}," The STM32 requires an external wireless communication module to implement Bluetooth functionality, which increases the complexity of the design.",{"type":13,"tag":14,"props":401,"children":402},{},[403,408],{"type":13,"tag":21,"props":404,"children":405},{},[406],{"type":18,"value":407},"2.Temperature Control Module:",{"type":18,"value":409}," Using the TB6612FNG to drive temperature control presents issues with heat dissipation and difficulties in achieving integration.",{"type":13,"tag":14,"props":411,"children":412},{},[413,418],{"type":13,"tag":21,"props":414,"children":415},{},[416],{"type":18,"value":417},"3.Detection Module:",{"type":18,"value":419}," The scheme of combining a fluorescence sensor with an optical path tends to have fluctuations in the excitation light source, and the optical path alignment is challenging and susceptible to environmental interference.",{"type":13,"tag":14,"props":421,"children":422},{},[423,428,430,435],{"type":13,"tag":21,"props":424,"children":425},{},[426],{"type":18,"value":427},"4.Biological Requirements:",{"type":18,"value":429}," The current design only allows for single detection, and frequent changes in the ",{"type":13,"tag":21,"props":431,"children":432},{},[433],{"type":18,"value":434},"reaction tube",{"type":18,"value":436}," can affect the stability of the detection environment, ultimately leading to inaccurate results.",{"type":13,"tag":14,"props":438,"children":439},{},[440],{"type":18,"value":441},"In response to the issues raised and the suggestions from the instructors, we have made improvements based on the original design.",{"type":13,"tag":14,"props":443,"children":444},{},[445],{"type":13,"tag":21,"props":446,"children":447},{},[448],{"type":13,"tag":56,"props":449,"children":451},{"alt":5,"src":450},"https://static.igem.wiki/teams/5358/hardware/9.png",[],{"type":13,"tag":14,"props":453,"children":454},{},[455],{"type":13,"tag":64,"props":456,"children":458},{"className":457},[67],[459],{"type":18,"value":460},"Figure 9 Improved Design Scheme",{"type":13,"tag":14,"props":462,"children":463},{},[464],{"type":18,"value":465},"Firstly, we replaced the STM32F103C8T6 with the Arduino Nano ESP32 chip as the MCU. The ESP32 features built-in Bluetooth communication capabilities and a rich set of peripheral interfaces. It supports the CMake and Ninja build systems, which will greatly simplify the design complexity. Its widespread adoption globally will also make it more universal and laying a foundation for future teams looking to design similar devices. For temperature control and detection, we replaced the original scheme with the TCM1040 temperature control module and the FM-4A digital fluorescence detection module from LIDE Technology, respectively. Compared to the original scheme, these modules offer more integrated features, further enhancing the accuracy and stability of the platform.",{"type":13,"tag":14,"props":467,"children":468},{},[469],{"type":13,"tag":56,"props":470,"children":472},{"alt":5,"src":471},"https://static.igem.wiki/teams/5358/hardware/10.png",[],{"type":13,"tag":14,"props":474,"children":475},{},[476],{"type":13,"tag":64,"props":477,"children":479},{"className":478},[67],[480],{"type":18,"value":481},"Figure 10 Improved Design Circuit Diagram",{"type":13,"tag":14,"props":483,"children":484},{},[485],{"type":18,"value":486},"To meet the demand for high-throughput detection, we proposed two solutions: a microfluidic chip rotation detection platform and a linear slide rail detection platform. After modeling analysis and feasibility analysis, we ultimately chose the linear slide rail detection platform and designed a brand-new enclosure for it.",{"type":13,"tag":14,"props":488,"children":489},{},[490],{"type":13,"tag":56,"props":491,"children":493},{"alt":5,"src":492},"https://static.igem.wiki/teams/5358/hardware/11.png",[],{"type":13,"tag":14,"props":495,"children":496},{},[497],{"type":13,"tag":64,"props":498,"children":500},{"className":499},[67],[501],{"type":18,"value":502},"Figure 11 Implementation Plan of the New Equipment",{"type":13,"tag":14,"props":504,"children":505},{},[506],{"type":13,"tag":56,"props":507,"children":509},{"alt":5,"src":508},"https://static.igem.wiki/teams/5358/engineering-success/new.png",[],{"type":13,"tag":14,"props":511,"children":512},{},[513],{"type":13,"tag":64,"props":514,"children":516},{"className":515},[67],[517],{"type":18,"value":518},"Figure 12 Model Diagram of the New Equipment",{"type":13,"tag":303,"props":520,"children":522},{"id":521},"goal-achievement",[523],{"type":18,"value":524},"Goal Achievement",{"type":13,"tag":526,"props":527,"children":529},"h4",{"id":528},"final-product",[530],{"type":18,"value":531},"Final Product",{"type":13,"tag":14,"props":533,"children":534},{},[535,537,542,544,549,551,556],{"type":18,"value":536},"The final system is primarily divided into a ",{"type":13,"tag":21,"props":538,"children":539},{},[540],{"type":18,"value":541},"sampling area",{"type":18,"value":543}," and a ",{"type":13,"tag":21,"props":545,"children":546},{},[547],{"type":18,"value":548},"detection area.",{"type":18,"value":550}," In actual use, the freeze-dried engineering bacteria will be mixed with multiple samples to be tested in separate reaction chambers for cultivation, awaiting detection.Upon receiving operational commands from the software, the device rapidly heats the aluminum alloy temperature-controlled slot in ",{"type":13,"tag":21,"props":552,"children":553},{},[554],{"type":18,"value":555},"the sampling area",{"type":18,"value":557}," and maintains a constant temperature, while also providing real-time feedback of the temperature and the operational status of the system to the mobile software interface via Bluetooth. Concurrently, the fluorescence detection module in the detection area begins to sequentially detect the fluorescence signals of each reaction chamber along the slide rail and provides real-time feedback to the software's visual interface. When the engineering bacteria grow to a certain extent, the intensity of the fluorescence signal will reflect the degree of endocrine disruptor effects.",{"type":13,"tag":559,"props":560,"children":562},"h5",{"id":561},"functional-testing",[563],{"type":18,"value":564},"Functional Testing",{"type":13,"tag":14,"props":566,"children":567},{},[568],{"type":18,"value":569},"Temperature Control Capability Test",{"type":13,"tag":14,"props":571,"children":572},{},[573],{"type":18,"value":574},"We conducted another temperature stability test on the latest version of the system. The results shown in Figure 12 indicate that the absolute difference between the average temperature and the set temperature is less than 0.3°C, suggesting that the stability of the improved temperature control system has been further enhanced. This can well meet practical requirements.",{"type":13,"tag":14,"props":576,"children":577},{},[578],{"type":13,"tag":64,"props":579,"children":581},{"className":580},[326,327],[582,586,587],{"type":13,"tag":56,"props":583,"children":585},{"alt":5,"src":584},"https://static.igem.wiki/teams/5358/hardware/13.png",[],{"type":18,"value":215},{"type":13,"tag":56,"props":588,"children":590},{"alt":5,"src":589},"https://static.igem.wiki/teams/5358/hardware/14.png",[],{"type":13,"tag":14,"props":592,"children":593},{},[594],{"type":13,"tag":64,"props":595,"children":597},{"className":596},[67],[598],{"type":18,"value":599},"Figure 13 Measurement Results of Temperature Fluctuation",{"type":13,"tag":559,"props":601,"children":603},{"id":602},"gradient-fluorescein-sodium-fluorescence-detection-verification",[604],{"type":18,"value":605},"Gradient Fluorescein Sodium Fluorescence Detection Verification",{"type":13,"tag":14,"props":607,"children":608},{},[609],{"type":18,"value":610},"The fluorescence validation test of the latest equipment shows that the tested Relative Fluorescence Units (RFU) values have a good linear relationship with the concentration gradient of fluorescein sodium. This indicates that the new fluorescence detection module can effectively reflect the concentration of substances, meeting the usage requirements.",{"type":13,"tag":14,"props":612,"children":613},{},[614],{"type":13,"tag":56,"props":615,"children":617},{"alt":5,"src":616},"https://static.igem.wiki/teams/5358/hardware/15.png",[],{"type":13,"tag":14,"props":619,"children":620},{},[621],{"type":13,"tag":64,"props":622,"children":624},{"className":623},[67],[625],{"type":18,"value":626},"Figure 14 Fluorescence Verification Experiment Fitting Curve",{"type":13,"tag":72,"props":628,"children":630},{"id":629},"android-software",[631],{"type":18,"value":632},"Android Software",{"type":13,"tag":14,"props":634,"children":635},{},[636],{"type":18,"value":637},"We have conducted functional testing on the already designed software. Initially, the software is imported onto an Android phone and the Bluetooth module is connected to check for a normal connection. The software is then opened to inspect whether the UI (User Interface) design is complete. Subsequently, the signals received from the module are used to test the graph plotting functionality to ensure it operates correctly, and the system is optimized based on the results.",{"type":13,"tag":72,"props":639,"children":641},{"id":640},"data-reception-verification",[642],{"type":18,"value":643},"Data Reception Verification",{"type":13,"tag":14,"props":645,"children":646},{},[647],{"type":18,"value":648},"Signals for initiation are transmitted from the mobile phone to the MCU via Bluetooth. As can be seen from the diagram, the hardware successfully starts up and returns a designated identifier.",{"type":13,"tag":72,"props":650,"children":652},{"id":651},"data-plotting-verification",[653],{"type":18,"value":654},"Data Plotting Verification",{"type":13,"tag":14,"props":656,"children":657},{},[658],{"type":18,"value":659},"During operation, the MCU sends data to the mobile phone via Bluetooth. The mobile phone then parses this data and plots graphs of time-temperature and time-fluorescence intensity. The diagrams indicate that the program has successfully implemented its functionalities.",{"type":13,"tag":72,"props":661,"children":663},{"id":662},"improvement",[664],{"type":18,"value":665},"Improvement",{"type":13,"tag":14,"props":667,"children":668},{},[669],{"type":18,"value":670},"Due to the addition of a linear slide rail for the movement of fluorescence detection in the second version of the hardware design, the signals obtained from the fluorescence detection have also changed. Therefore, the program is required to implement the ability to distinguish between the fluorescence intensity pulse peaks of different channels and to obtain their respective maximum values (theoretically, the fluorescence intensity at the bottom of the tube) for plotting.",{"type":13,"tag":72,"props":672,"children":674},{"id":673},"array-splitting-and-plotting-verification",[675],{"type":18,"value":676},"Array Splitting and Plotting Verification",{"type":13,"tag":14,"props":678,"children":679},{},[680],{"type":18,"value":681},"We can observe a set of data with multiple pulse peaks returned by the MCU. The software successfully splits this data into several different arrays and obtains the maximum value from each array for plotting.",{"type":13,"tag":72,"props":683,"children":685},{"id":684},"summary",[686],{"type":18,"value":687},"Summary",{"type":13,"tag":14,"props":689,"children":690},{},[691],{"type":18,"value":692},"The final product and its testing demonstrate that the proactive detection system for EDCs we have developed is compact, portable, and user-friendly. It effectively controls the standardization of cultivation conditions, enabling rapid screening and assessment of EDCs in wastewater, significantly reducing reliance on laboratory equipment and professional personnel. Additionally, the system's high-throughput operation allows for the simultaneous detection of multiple samples, thereby conducting sample testing more efficiently.",{"type":13,"tag":14,"props":694,"children":695},{},[696],{"type":18,"value":697},"In designing the hardware, we employed engineering and project design approaches, taking into account target requirements and user feedback. Our instructor, Professor Li Xiaoqiong, recommended replacing the STM32 chip with the ESP32 chip, which will aid in enhancing the readability and versatility of the design. During testing and actual use, we collected feedback from operators and utilized a high-throughput platform, facilitating users to test multiple samples conveniently.",{"type":13,"tag":14,"props":699,"children":700},{},[701],{"type":18,"value":702},"To facilitate user operation, we have added detailed user instructions in the App and will record demonstration videos for ease of use. At the same time, we have organized detailed system design blueprints and modeling files, as well as detailed component selection and manufacturing methods. You can contact us on the contribution page to obtain the relevant documents, thereby assisting other participants in replicating our device. Our main controller and major systems utilize common integrated units, while other parts are made using industrial processing and 3D printing techniques, making them easy to replicate for reference and implementation by other teams.",{"type":13,"tag":14,"props":704,"children":705},{},[706],{"type":13,"tag":64,"props":707,"children":710},{"className":708},[709],"text-2xl",[711],{"type":13,"tag":712,"props":713,"children":714},"em",{},[715],{"type":18,"value":716},"References",{"type":13,"tag":252,"props":718,"children":720},{":class":719},"reference",[721],{"type":13,"tag":722,"props":723,"children":724},"ol",{},[725],{"type":13,"tag":726,"props":727,"children":728},"li",{},[729,731,736],{"type":18,"value":730},"Wang Y, Chen H, Lin K, et al. Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device",{"type":13,"tag":64,"props":732,"children":733},{},[734],{"type":18,"value":735},"j",{"type":18,"value":737},". Nature Communications, 2024, 15(1):3279.",{"title":5,"searchDepth":739,"depth":740,"links":741},1,3,[742,744,749,755,756,757,758,759,760],{"id":74,"depth":743,"text":77},2,{"id":121,"depth":743,"text":124,"children":745},[746,747,748],{"id":305,"depth":740,"text":308},{"id":311,"depth":740,"text":314},{"id":349,"depth":740,"text":352},{"id":381,"depth":743,"text":384,"children":750},[751],{"id":521,"depth":740,"text":524,"children":752},[753],{"id":528,"depth":754,"text":531},4,{"id":629,"depth":743,"text":632},{"id":640,"depth":743,"text":643},{"id":651,"depth":743,"text":654},{"id":662,"depth":743,"text":665},{"id":673,"depth":743,"text":676},{"id":684,"depth":743,"text":687},"markdown","content:drylab-hardware.md","content","drylab-hardware.md","drylab-hardware","md",1727868995313]
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These methods are time-consuming and costly.",{"type":10,"children":11,"toc":738},"root",[12,28,40,52,60,71,78,90,102,114,119,125,132,141,148,157,197,223,234,239,251,302,309,315,320,338,347,353,358,365,374,379,385,390,400,410,420,437,442,452,461,466,473,482,487,494,503,510,519,525,532,558,565,570,575,591,600,606,611,618,627,633,638,644,649,655,660,666,671,677,682,688,693,698,703,717],{"type":13,"tag":14,"props":15,"children":16},"element","p",{},[17,20,26],{"type":18,"value":19},"text","This year, the core objective of the BIT team is to develop ",{"type":13,"tag":21,"props":22,"children":23},"strong",{},[24],{"type":18,"value":25},"a initiative detection system for EDCs in aquatic environments based on E.coli biosensors.",{"type":18,"value":27}," Traditional detection methods, such as chromatography and immunoassays, require cumbersome procedures like sampling, pre-treatment, and laboratory examination, in addition to expensive laboratory equipment (e.g., chromatographs, microplate reader) and professional experimental techniques. These methods are time-consuming and costly.",{"type":13,"tag":14,"props":29,"children":30},{},[31,33,38],{"type":18,"value":32},"As EDCs continue to threaten aquatic environments and the quality of drinking water for residents, it is crucial to achieve portable detection and rapid response to EDCs. To meet the requirement, our goal is to develop a low costing, highly integrated, compact, portable, and user-friendly hardware platform that can be combined with smartphone software to automate the cultivation of engineering bacteria and the analysis of fluorescence ",{"type":13,"tag":21,"props":34,"children":35},{},[36],{"type":18,"value":37},"detection",{"type":18,"value":39},".",{"type":13,"tag":14,"props":41,"children":42},{},[43,45,50],{"type":18,"value":44},"To ensure that the hardware device truly meets the needs of system construction, we have adopted a typical engineering and project design approach, namely: ",{"type":13,"tag":21,"props":46,"children":47},{},[48],{"type":18,"value":49},"demand analysis, scheme proposal, feasibility verification, feedback , system improvement, and goal achievement.",{"type":18,"value":51}," This process is an iterative one, and after several cycles, it ultimately meets all the needs and objectives.",{"type":13,"tag":14,"props":53,"children":54},{},[55],{"type":13,"tag":56,"props":57,"children":59},"img",{"alt":5,"src":58},"https://static.igem.wiki/teams/5358/hardware/1.png",[],{"type":13,"tag":14,"props":61,"children":62},{},[63],{"type":13,"tag":64,"props":65,"children":68},"span",{"className":66},[67],"fig-title",[69],{"type":18,"value":70},"Figure 1 Hardware Design Flowchart",{"type":13,"tag":72,"props":73,"children":75},"h2",{"id":74},"demand-analysis",[76],{"type":18,"value":77},"Demand Analysis",{"type":13,"tag":14,"props":79,"children":80},{},[81,83,88],{"type":18,"value":82},"To ensure the stability and accuracy of ",{"type":13,"tag":21,"props":84,"children":85},{},[86],{"type":18,"value":87},"fluorescence detection",{"type":18,"value":89},", it is necessary to standardize the detection process and use high sensitive detection units.",{"type":13,"tag":14,"props":91,"children":92},{},[93,95,100],{"type":18,"value":94},"Standardization of the detection process requires a continuous and stable chemical and physical environment during the resuscitation of engineering bacteria and fluorescence detection. The standardization of the chemical environment is mainly ensured by the quantified operation of experimental steps (including control of sample volume and the number of engineering bacteria, control of the chemical composition and concentration of the culture medium), and the standardization of the physical environment is mainly ensured by the stability of light source, temperature, and humidity. Among them, light exposure problem has been solved by the sealed and light-proof nature of the device, and the humidity in the sealed environment is mainly determined by temperature. The standardization of the detection process mainly aims to achieve ",{"type":13,"tag":21,"props":96,"children":97},{},[98],{"type":18,"value":99},"constant temperature control",{"type":18,"value":101}," of the hardware device.",{"type":13,"tag":14,"props":103,"children":104},{},[105,107,112],{"type":18,"value":106},"Since the concentration of ",{"type":13,"tag":21,"props":108,"children":109},{},[110],{"type":18,"value":111},"EDCs",{"type":18,"value":113}," in the detection samples is very low，which also refer to the low intensity of fluorescence, coupled with the portability requirements of the equipment, the detection method required must be high sensitive and can be integrated into small devices. Common chemical detection methods cannot meet the requirements, so adopting a more sensitive, portable, and efficient detection method is crucial for the realization of our POCT system.",{"type":13,"tag":14,"props":115,"children":116},{},[117],{"type":18,"value":118},"In order to enable smartphone software to automate the control of hardware devices and visualize the detection results, the construction of hardware devices needs to consider the transmission of data between software and hardware.",{"type":13,"tag":72,"props":120,"children":122},{"id":121},"scheme-proposal",[123],{"type":18,"value":124},"Scheme Proposal",{"type":13,"tag":14,"props":126,"children":127},{},[128],{"type":13,"tag":56,"props":129,"children":131},{"alt":5,"src":130},"https://static.igem.wiki/teams/5358/hardware/2.png",[],{"type":13,"tag":14,"props":133,"children":134},{},[135],{"type":13,"tag":64,"props":136,"children":138},{"className":137},[67],[139],{"type":18,"value":140},"Figure 2 First Edition Design Scheme",{"type":13,"tag":14,"props":142,"children":143},{},[144],{"type":13,"tag":56,"props":145,"children":147},{"alt":5,"src":146},"https://static.igem.wiki/teams/5358/hardware/3.png",[],{"type":13,"tag":14,"props":149,"children":150},{},[151],{"type":13,"tag":64,"props":152,"children":154},{"className":153},[67],[155],{"type":18,"value":156},"Figure 3 Circuit Diagram",{"type":13,"tag":14,"props":158,"children":159},{},[160,162,167,169,174,176,181,183,188,190,195],{"type":18,"value":161},"In the construction of the ",{"type":13,"tag":21,"props":163,"children":164},{},[165],{"type":18,"value":166},"temperature control module",{"type":18,"value":168},", we employed an NTC thermistor as the ",{"type":13,"tag":21,"props":170,"children":171},{},[172],{"type":18,"value":173},"temperature sensor",{"type":18,"value":175},", utilizing heating FILM and fan as the heat and cold sources respectively. To achieve ",{"type":13,"tag":21,"props":177,"children":178},{},[179],{"type":18,"value":180},"PID regulation",{"type":18,"value":182}," for ",{"type":13,"tag":21,"props":184,"children":185},{},[186],{"type":18,"value":187},"temperature sensing and control",{"type":18,"value":189},", we used the PWM wave output from the ",{"type":13,"tag":21,"props":191,"children":192},{},[193],{"type":18,"value":194},"TB6612FNG motor drive",{"type":18,"value":196}," to adjust the power of the fan and heating FILM, thereby maintaining dynamic temperature stability.",{"type":13,"tag":14,"props":198,"children":199},{},[200,202,207,209,214,216,221],{"type":18,"value":201},"For the selection of the detection scheme, we comprehensively considered the detection accuracy and the portability of the device, ultimately choosing the ",{"type":13,"tag":21,"props":203,"children":204},{},[205],{"type":18,"value":206},"fluorescence detection module",{"type":18,"value":208},". We initially selected the AS7341 as ",{"type":13,"tag":21,"props":210,"children":211},{},[212],{"type":18,"value":213},"fluorescence sensor",{"type":18,"value":215}," ",{"type":13,"tag":64,"props":217,"children":218},{},[219],{"type":18,"value":220},"1",{"type":18,"value":222},". Concurrently, to ensure consistent fluorescence generation, we redesigned and optimized the optical path.",{"type":13,"tag":14,"props":224,"children":225},{},[226,228,233],{"type":18,"value":227},"After the marked fluorescence is excited by a blue LED, the fluorescence sensor collect and record real-time fluorescence signals, which can be visualized on the mobile software via a ",{"type":13,"tag":21,"props":229,"children":230},{},[231],{"type":18,"value":232},"communication module",{"type":18,"value":39},{"type":13,"tag":14,"props":235,"children":236},{},[237],{"type":18,"value":238},"For the communication module, we used the HC-05 Bluetooth module to connect the hardware system with the mobile software, enabling real-time monitoring of the hardware device's operating status and feedback of the detection results.",{"type":13,"tag":14,"props":240,"children":241},{},[242,244,249],{"type":18,"value":243},"In the initial version of the scheme, we used an STM32 chip as the ",{"type":13,"tag":21,"props":245,"children":246},{},[247],{"type":18,"value":248},"microcontroller Unit(MCU)",{"type":18,"value":250},", connecting temperature control module, fluorescence detection module, and Bluetooth communication module, ensuring the normal operation of the device through programming instructions.",{"type":13,"tag":252,"props":253,"children":255},"mdc-wrap",{":class":254},"table-fig",[256],{"type":13,"tag":257,"props":258,"children":259},"table",{},[260,284],{"type":13,"tag":261,"props":262,"children":263},"thead",{},[264],{"type":13,"tag":265,"props":266,"children":267},"tr",{},[268,277],{"type":13,"tag":269,"props":270,"children":272},"th",{"align":271},"center",[273],{"type":13,"tag":56,"props":274,"children":276},{"alt":5,"src":275},"https://static.igem.wiki/teams/5358/hardware/4.png",[],{"type":13,"tag":269,"props":278,"children":279},{"align":271},[280],{"type":13,"tag":56,"props":281,"children":283},{"alt":5,"src":282},"https://static.igem.wiki/teams/5358/hardware/5.png",[],{"type":13,"tag":285,"props":286,"children":287},"tbody",{},[288],{"type":13,"tag":265,"props":289,"children":290},{},[291,297],{"type":13,"tag":292,"props":293,"children":294},"td",{"align":271},[295],{"type":18,"value":296},"Figure 5:  First Edition Implementation Scheme",{"type":13,"tag":292,"props":298,"children":299},{"align":271},[300],{"type":18,"value":301},"Figure 6: First Edition Equipment Model Diagram",{"type":13,"tag":303,"props":304,"children":306},"h3",{"id":305},"feasibility-verification",[307],{"type":18,"value":308},"Feasibility Verification",{"type":13,"tag":303,"props":310,"children":312},{"id":311},"temperature-stability-verification",[313],{"type":18,"value":314},"Temperature Stability Verification",{"type":13,"tag":14,"props":316,"children":317},{},[318],{"type":18,"value":319},"To verify the constant temperature effect of the temperature control system, we designed a temperature stability test experiment. When the temperature first reached 37°C, the temperature was recorded every 10 seconds for an additional 60 seconds. From the results shown in Figure 6, it can be concluded that the absolute difference between the average temperature and the set temperature was no greater than 1.1°C, and the temperature curve consistently fluctuated around 37°C. This indicates that the temperature control system can achieve dynamic temperature regulation, capable of maintaining the temperature close to 37°C. However, this temperature control method generates relatively large temperature fluctuations.",{"type":13,"tag":14,"props":321,"children":322},{},[323],{"type":13,"tag":64,"props":324,"children":328},{"className":325},[326,327],"flex","gap-4",[329,333,334],{"type":13,"tag":56,"props":330,"children":332},{"alt":5,"src":331},"https://static.igem.wiki/teams/5358/hardware/6.png",[],{"type":18,"value":215},{"type":13,"tag":56,"props":335,"children":337},{"alt":5,"src":336},"https://static.igem.wiki/teams/5358/hardware/7.png",[],{"type":13,"tag":14,"props":339,"children":340},{},[341],{"type":13,"tag":64,"props":342,"children":344},{"className":343},[67],[345],{"type":18,"value":346},"Figure 7 Measurement Results of  Initial Scheme's Temperature Stability",{"type":13,"tag":303,"props":348,"children":350},{"id":349},"gradient-fluoresce-in-sodium-fluorescence-detection-verification",[351],{"type":18,"value":352},"Gradient Fluoresce in Sodium Fluorescence Detection Verification",{"type":13,"tag":14,"props":354,"children":355},{},[356],{"type":18,"value":357},"The AS7341 fluorescence sensor, by integrating filters of different wavelength bands, achieves the function of detecting light intensity (Lux) at specific wavelengths, which is equivalent to detecting the corresponding fluorescence intensity. To verify whether this sensor can correctly reflect the signal from the sample, we designed an experiment to explore the relationship between the concentration gradient of fluoresce in sodium and Lux. We prepared a series of fluoresce in sodium solutions with different gradients in a 2mL centrifuge tube for signal collection using the AS734. The results shown in Figure 8 indicate that the measured Lux values have a good linear correlation with the concentration gradient of fluoresce in sodium, demonstrating that our fluorescence detection module can accurately reflect the concentration of substances and is capable of conducting fluorescence detection.",{"type":13,"tag":14,"props":359,"children":360},{},[361],{"type":13,"tag":56,"props":362,"children":364},{"alt":5,"src":363},"https://static.igem.wiki/teams/5358/hardware/8.png",[],{"type":13,"tag":14,"props":366,"children":367},{},[368],{"type":13,"tag":64,"props":369,"children":371},{"className":370},[67],[372],{"type":18,"value":373},"Figure 8 Initial Scheme's Fluorescence Verification Fitting Curve",{"type":13,"tag":14,"props":375,"children":376},{},[377],{"type":18,"value":378},"The above feasibility verification demonstrates that the temperature control module and fluorescence detection module designed in the first version of the scheme can meet the design requirements and are feasible.",{"type":13,"tag":72,"props":380,"children":382},{"id":381},"feedback",[383],{"type":18,"value":384},"Feedback",{"type":13,"tag":14,"props":386,"children":387},{},[388],{"type":18,"value":389},"We engaged in in-depth discussions with the biology team, design team, and professional instructors regarding our hardware design, obtaining extremely valuable feedback. This feedback is instrumental in guiding our further improvements.",{"type":13,"tag":14,"props":391,"children":392},{},[393,398],{"type":13,"tag":21,"props":394,"children":395},{},[396],{"type":18,"value":397},"1.MCU:",{"type":18,"value":399}," The STM32 requires an external wireless communication module to implement Bluetooth functionality, which increases the complexity of the design.",{"type":13,"tag":14,"props":401,"children":402},{},[403,408],{"type":13,"tag":21,"props":404,"children":405},{},[406],{"type":18,"value":407},"2.Temperature Control Module:",{"type":18,"value":409}," Using the TB6612FNG to drive temperature control presents issues with heat dissipation and difficulties in achieving integration.",{"type":13,"tag":14,"props":411,"children":412},{},[413,418],{"type":13,"tag":21,"props":414,"children":415},{},[416],{"type":18,"value":417},"3.Detection Module:",{"type":18,"value":419}," The scheme of combining a fluorescence sensor with an optical path tends to have fluctuations in the excitation light source, and the optical path alignment is challenging and susceptible to environmental interference.",{"type":13,"tag":14,"props":421,"children":422},{},[423,428,430,435],{"type":13,"tag":21,"props":424,"children":425},{},[426],{"type":18,"value":427},"4.Biological Requirements:",{"type":18,"value":429}," The current design only allows for single detection, and frequent changes in the ",{"type":13,"tag":21,"props":431,"children":432},{},[433],{"type":18,"value":434},"reaction tube",{"type":18,"value":436}," can affect the stability of the detection environment, ultimately leading to inaccurate results.",{"type":13,"tag":14,"props":438,"children":439},{},[440],{"type":18,"value":441},"In response to the issues raised and the suggestions from the instructors, we have made improvements based on the original design.",{"type":13,"tag":14,"props":443,"children":444},{},[445],{"type":13,"tag":21,"props":446,"children":447},{},[448],{"type":13,"tag":56,"props":449,"children":451},{"alt":5,"src":450},"https://static.igem.wiki/teams/5358/hardware/9.png",[],{"type":13,"tag":14,"props":453,"children":454},{},[455],{"type":13,"tag":64,"props":456,"children":458},{"className":457},[67],[459],{"type":18,"value":460},"Figure 9 Improved Design Scheme",{"type":13,"tag":14,"props":462,"children":463},{},[464],{"type":18,"value":465},"Firstly, we replaced the STM32F103C8T6 with the Arduino Nano ESP32 chip as the MCU. The ESP32 features built-in Bluetooth communication capabilities and a rich set of peripheral interfaces. It supports the CMake and Ninja build systems, which will greatly simplify the design complexity. Its widespread adoption globally will also make it more universal and laying a foundation for future teams looking to design similar devices. For temperature control and detection, we replaced the original scheme with the TCM1040 temperature control module and the FM-4A digital fluorescence detection module from LIDE Technology, respectively. Compared to the original scheme, these modules offer more integrated features, further enhancing the accuracy and stability of the platform.",{"type":13,"tag":14,"props":467,"children":468},{},[469],{"type":13,"tag":56,"props":470,"children":472},{"alt":5,"src":471},"https://static.igem.wiki/teams/5358/hardware/10.png",[],{"type":13,"tag":14,"props":474,"children":475},{},[476],{"type":13,"tag":64,"props":477,"children":479},{"className":478},[67],[480],{"type":18,"value":481},"Figure 10 Improved Design Circuit Diagram",{"type":13,"tag":14,"props":483,"children":484},{},[485],{"type":18,"value":486},"To meet the demand for high-throughput detection, we proposed two solutions: a microfluidic chip rotation detection platform and a linear slide rail detection platform. After modeling analysis and feasibility analysis, we ultimately chose the linear slide rail detection platform and designed a brand-new enclosure for it.",{"type":13,"tag":14,"props":488,"children":489},{},[490],{"type":13,"tag":56,"props":491,"children":493},{"alt":5,"src":492},"https://static.igem.wiki/teams/5358/hardware/11.png",[],{"type":13,"tag":14,"props":495,"children":496},{},[497],{"type":13,"tag":64,"props":498,"children":500},{"className":499},[67],[501],{"type":18,"value":502},"Figure 11 Implementation Plan of the New Equipment",{"type":13,"tag":14,"props":504,"children":505},{},[506],{"type":13,"tag":56,"props":507,"children":509},{"alt":5,"src":508},"https://static.igem.wiki/teams/5358/engineering-success/new.png",[],{"type":13,"tag":14,"props":511,"children":512},{},[513],{"type":13,"tag":64,"props":514,"children":516},{"className":515},[67],[517],{"type":18,"value":518},"Figure 12 Model Diagram of the New Equipment",{"type":13,"tag":303,"props":520,"children":522},{"id":521},"goal-achievement",[523],{"type":18,"value":524},"Goal Achievement",{"type":13,"tag":526,"props":527,"children":529},"h4",{"id":528},"final-product",[530],{"type":18,"value":531},"Final Product",{"type":13,"tag":14,"props":533,"children":534},{},[535,537,542,544,549,551,556],{"type":18,"value":536},"The final system is primarily divided into a ",{"type":13,"tag":21,"props":538,"children":539},{},[540],{"type":18,"value":541},"sampling area",{"type":18,"value":543}," and a ",{"type":13,"tag":21,"props":545,"children":546},{},[547],{"type":18,"value":548},"detection area.",{"type":18,"value":550}," In actual use, the freeze-dried engineering bacteria will be mixed with multiple samples to be tested in separate reaction chambers for cultivation, awaiting detection.Upon receiving operational commands from the software, the device rapidly heats the aluminum alloy temperature-controlled slot in ",{"type":13,"tag":21,"props":552,"children":553},{},[554],{"type":18,"value":555},"the sampling area",{"type":18,"value":557}," and maintains a constant temperature, while also providing real-time feedback of the temperature and the operational status of the system to the mobile software interface via Bluetooth. Concurrently, the fluorescence detection module in the detection area begins to sequentially detect the fluorescence signals of each reaction chamber along the slide rail and provides real-time feedback to the software's visual interface. When the engineering bacteria grow to a certain extent, the intensity of the fluorescence signal will reflect the degree of endocrine disruptor effects.",{"type":13,"tag":559,"props":560,"children":562},"h5",{"id":561},"functional-testing",[563],{"type":18,"value":564},"Functional Testing",{"type":13,"tag":14,"props":566,"children":567},{},[568],{"type":18,"value":569},"Temperature Control Capability Test",{"type":13,"tag":14,"props":571,"children":572},{},[573],{"type":18,"value":574},"We conducted another temperature stability test on the latest version of the system. The results shown in Figure 12 indicate that the absolute difference between the average temperature and the set temperature is less than 0.3°C, suggesting that the stability of the improved temperature control system has been further enhanced. This can well meet practical requirements.",{"type":13,"tag":14,"props":576,"children":577},{},[578],{"type":13,"tag":64,"props":579,"children":581},{"className":580},[326,327],[582,586,587],{"type":13,"tag":56,"props":583,"children":585},{"alt":5,"src":584},"https://static.igem.wiki/teams/5358/hardware/13.png",[],{"type":18,"value":215},{"type":13,"tag":56,"props":588,"children":590},{"alt":5,"src":589},"https://static.igem.wiki/teams/5358/hardware/14.png",[],{"type":13,"tag":14,"props":592,"children":593},{},[594],{"type":13,"tag":64,"props":595,"children":597},{"className":596},[67],[598],{"type":18,"value":599},"Figure 13 Measurement Results of Temperature Fluctuation",{"type":13,"tag":559,"props":601,"children":603},{"id":602},"gradient-fluorescein-sodium-fluorescence-detection-verification",[604],{"type":18,"value":605},"Gradient Fluorescein Sodium Fluorescence Detection Verification",{"type":13,"tag":14,"props":607,"children":608},{},[609],{"type":18,"value":610},"The fluorescence validation test of the latest equipment shows that the tested Relative Fluorescence Units (RFU) values have a good linear relationship with the concentration gradient of fluorescein sodium. This indicates that the new fluorescence detection module can effectively reflect the concentration of substances, meeting the usage requirements.",{"type":13,"tag":14,"props":612,"children":613},{},[614],{"type":13,"tag":56,"props":615,"children":617},{"alt":5,"src":616},"https://static.igem.wiki/teams/5358/hardware/15.png",[],{"type":13,"tag":14,"props":619,"children":620},{},[621],{"type":13,"tag":64,"props":622,"children":624},{"className":623},[67],[625],{"type":18,"value":626},"Figure 14 Fluorescence Verification Experiment Fitting Curve",{"type":13,"tag":72,"props":628,"children":630},{"id":629},"android-software",[631],{"type":18,"value":632},"Android Software",{"type":13,"tag":14,"props":634,"children":635},{},[636],{"type":18,"value":637},"We have conducted functional testing on the already designed software. Initially, the software is imported onto an Android phone and the Bluetooth module is connected to check for a normal connection. The software is then opened to inspect whether the UI (User Interface) design is complete. Subsequently, the signals received from the module are used to test the graph plotting functionality to ensure it operates correctly, and the system is optimized based on the results.",{"type":13,"tag":72,"props":639,"children":641},{"id":640},"data-reception-verification",[642],{"type":18,"value":643},"Data Reception Verification",{"type":13,"tag":14,"props":645,"children":646},{},[647],{"type":18,"value":648},"Signals for initiation are transmitted from the mobile phone to the MCU via Bluetooth. As can be seen from the diagram, the hardware successfully starts up and returns a designated identifier.",{"type":13,"tag":72,"props":650,"children":652},{"id":651},"data-plotting-verification",[653],{"type":18,"value":654},"Data Plotting Verification",{"type":13,"tag":14,"props":656,"children":657},{},[658],{"type":18,"value":659},"During operation, the MCU sends data to the mobile phone via Bluetooth. The mobile phone then parses this data and plots graphs of time-temperature and time-fluorescence intensity. The diagrams indicate that the program has successfully implemented its functionalities.",{"type":13,"tag":72,"props":661,"children":663},{"id":662},"improvement",[664],{"type":18,"value":665},"Improvement",{"type":13,"tag":14,"props":667,"children":668},{},[669],{"type":18,"value":670},"Due to the addition of a linear slide rail for the movement of fluorescence detection in the second version of the hardware design, the signals obtained from the fluorescence detection have also changed. Therefore, the program is required to implement the ability to distinguish between the fluorescence intensity pulse peaks of different channels and to obtain their respective maximum values (theoretically, the fluorescence intensity at the bottom of the tube) for plotting.",{"type":13,"tag":72,"props":672,"children":674},{"id":673},"array-splitting-and-plotting-verification",[675],{"type":18,"value":676},"Array Splitting and Plotting Verification",{"type":13,"tag":14,"props":678,"children":679},{},[680],{"type":18,"value":681},"We can observe a set of data with multiple pulse peaks returned by the MCU. The software successfully splits this data into several different arrays and obtains the maximum value from each array for plotting.",{"type":13,"tag":72,"props":683,"children":685},{"id":684},"summary",[686],{"type":18,"value":687},"Summary",{"type":13,"tag":14,"props":689,"children":690},{},[691],{"type":18,"value":692},"The final product and its testing demonstrate that the proactive detection system for EDCs we have developed is compact, portable, and user-friendly. It effectively controls the standardization of cultivation conditions, enabling rapid screening and assessment of EDCs in wastewater, significantly reducing reliance on laboratory equipment and professional personnel. Additionally, the system's high-throughput operation allows for the simultaneous detection of multiple samples, thereby conducting sample testing more efficiently.",{"type":13,"tag":14,"props":694,"children":695},{},[696],{"type":18,"value":697},"In designing the hardware, we employed engineering and project design approaches, taking into account target requirements and user feedback. Our instructor, Professor Li Xiaoqiong, recommended replacing the STM32 chip with the ESP32 chip, which will aid in enhancing the readability and versatility of the design. During testing and actual use, we collected feedback from operators and utilized a high-throughput platform, facilitating users to test multiple samples conveniently.",{"type":13,"tag":14,"props":699,"children":700},{},[701],{"type":18,"value":702},"To facilitate user operation, we have added detailed user instructions in the App and will record demonstration videos for ease of use. At the same time, we have organized detailed system design blueprints and modeling files, as well as detailed component selection and manufacturing methods. You can contact us on the contribution page to obtain the relevant documents, thereby assisting other participants in replicating our device. Our main controller and major systems utilize common integrated units, while other parts are made using industrial processing and 3D printing techniques, making them easy to replicate for reference and implementation by other teams.",{"type":13,"tag":14,"props":704,"children":705},{},[706],{"type":13,"tag":64,"props":707,"children":710},{"className":708},[709],"text-2xl",[711],{"type":13,"tag":712,"props":713,"children":714},"em",{},[715],{"type":18,"value":716},"References",{"type":13,"tag":252,"props":718,"children":720},{":class":719},"reference",[721],{"type":13,"tag":722,"props":723,"children":724},"ol",{},[725],{"type":13,"tag":726,"props":727,"children":728},"li",{},[729,731,736],{"type":18,"value":730},"Wang Y, Chen H, Lin K, et al. Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device",{"type":13,"tag":64,"props":732,"children":733},{},[734],{"type":18,"value":735},"j",{"type":18,"value":737},". Nature Communications, 2024, 15(1):3279.",{"title":5,"searchDepth":739,"depth":740,"links":741},1,3,[742,744,749,755,756,757,758,759,760],{"id":74,"depth":743,"text":77},2,{"id":121,"depth":743,"text":124,"children":745},[746,747,748],{"id":305,"depth":740,"text":308},{"id":311,"depth":740,"text":314},{"id":349,"depth":740,"text":352},{"id":381,"depth":743,"text":384,"children":750},[751],{"id":521,"depth":740,"text":524,"children":752},[753],{"id":528,"depth":754,"text":531},4,{"id":629,"depth":743,"text":632},{"id":640,"depth":743,"text":643},{"id":651,"depth":743,"text":654},{"id":662,"depth":743,"text":665},{"id":673,"depth":743,"text":676},{"id":684,"depth":743,"text":687},"markdown","content:drylab-hardware.md","content","drylab-hardware.md","drylab-hardware","md",1727871092354]
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20px;position:relative}.el-cascader-node.is-selectable.in-active-path{color:var(--el-cascader-menu-text-color)}.el-cascader-node.in-active-path,.el-cascader-node.is-active,.el-cascader-node.is-selectable.in-checked-path{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader-node:not(.is-disabled){cursor:pointer}.el-cascader-node:not(.is-disabled):focus,.el-cascader-node:not(.is-disabled):hover{background:var(--el-cascader-node-background-hover)}.el-cascader-node.is-disabled{color:var(--el-cascader-node-color-disabled);cursor:not-allowed}.el-cascader-node__prefix{left:10px;position:absolute}.el-cascader-node__postfix{position:absolute;right:10px}.el-cascader-node__label{flex:1;overflow:hidden;padding:0 8px;text-align:left;text-overflow:ellipsis;white-space:nowrap}.el-cascader-node>.el-checkbox,.el-cascader-node>.el-radio{margin-right:0}.el-cascader-node>.el-radio .el-radio__label{padding-left:0}.el-cascader{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);display:inline-block;font-size:var(--el-font-size-base);line-height:32px;outline:none;position:relative;vertical-align:middle}.el-cascader:not(.is-disabled):hover .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset;cursor:pointer}.el-cascader .el-input{cursor:pointer;display:flex}.el-cascader .el-input .el-input__inner{cursor:pointer;text-overflow:ellipsis}.el-cascader .el-input .el-input__suffix-inner .el-icon{height:calc(100% - 2px)}.el-cascader .el-input .el-input__suffix-inner .el-icon svg{vertical-align:middle}.el-cascader .el-input .icon-arrow-down{font-size:14px;transition:transform var(--el-transition-duration)}.el-cascader .el-input .icon-arrow-down.is-reverse{transform:rotate(180deg)}.el-cascader .el-input .icon-circle-close:hover{color:var(--el-input-clear-hover-color,var(--el-text-color-secondary))}.el-cascader .el-input.is-focus .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-focus-border-color,var(--el-color-primary)) inset}.el-cascader--large{font-size:14px;line-height:40px}.el-cascader--small{font-size:12px;line-height:24px}.el-cascader.is-disabled .el-cascader__label{color:var(--el-disabled-text-color);z-index:calc(var(--el-index-normal) + 1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper .el-popper__arrow:before{border:var(--el-cascader-menu-border)}.el-cascader__dropdown.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper{box-shadow:var(--el-cascader-menu-shadow)}.el-cascader__tags{box-sizing:border-box;display:flex;flex-wrap:wrap;left:0;line-height:normal;position:absolute;right:30px;text-align:left;top:50%;transform:translateY(-50%)}.el-cascader__tags .el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags .el-tag{align-items:center;background:var(--el-fill-color);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag.el-tag--dark,.el-cascader__collapse-tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__collapse-tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__collapse-tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__collapse-tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__suggestion-panel{border-radius:var(--el-cascader-menu-radius)}.el-cascader__suggestion-list{color:var(--el-cascader-menu-text-color);font-size:var(--el-font-size-base);margin:0;max-height:204px;padding:6px 0;text-align:center}.el-cascader__suggestion-item{align-items:center;cursor:pointer;display:flex;height:34px;justify-content:space-between;outline:none;padding:0 15px;text-align:left}.el-cascader__suggestion-item:focus,.el-cascader__suggestion-item:hover{background:var(--el-cascader-node-background-hover)}.el-cascader__suggestion-item.is-checked{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader__suggestion-item>span{margin-right:10px}.el-cascader__empty-text{color:var(--el-cascader-color-empty);margin:10px 0}.el-cascader__search-input{background:transparent;border:none;box-sizing:border-box;color:var(--el-cascader-menu-text-color);flex:1;height:24px;margin:2px 0 2px 11px;min-width:60px;outline:none;padding:0}.el-cascader__search-input::-moz-placeholder{color:transparent}.el-cascader__search-input::placeholder{color:transparent}.el-check-tag{background-color:var(--el-color-info-light-9);border-radius:var(--el-border-radius-base);color:var(--el-color-info);cursor:pointer;display:inline-block;font-size:var(--el-font-size-base);font-weight:700;line-height:var(--el-font-size-base);padding:7px 15px;transition:var(--el-transition-all)}.el-check-tag:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--primary.is-checked{background-color:var(--el-color-primary-light-8);color:var(--el-color-primary)}.el-check-tag.el-check-tag--primary.is-checked:hover{background-color:var(--el-color-primary-light-7)}.el-check-tag.el-check-tag--primary.is-checked.is-disabled{background-color:var(--el-color-primary-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-checked.is-disabled:hover{background-color:var(--el-color-primary-light-8)}.el-check-tag.el-check-tag--primary.is-disabled{background-color:var(--el-color-info-light-9);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-check-tag.el-check-tag--success.is-checked{background-color:var(--el-color-success-light-8);color:var(--el-color-success)}.el-check-tag.el-check-tag--success.is-checked:hover{background-color:var(--el-color-success-light-7)}.el-check-tag.el-check-tag--success.is-checked.is-disabled{background-color:var(--el-color-success-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-checked.is-disabled:hover{background-color:var(--el-color-success-light-8)}.el-check-tag.el-check-tag--success.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-disabled,.el-check-tag.el-check-tag--success.is-disabled:hover{background-color:var(--el-color-success-light-9)}.el-check-tag.el-check-tag--warning.is-checked{background-color:var(--el-color-warning-light-8);color:var(--el-color-warning)}.el-check-tag.el-check-tag--warning.is-checked:hover{background-color:var(--el-color-warning-light-7)}.el-check-tag.el-check-tag--warning.is-checked.is-disabled{background-color:var(--el-color-warning-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-checked.is-disabled:hover{background-color:var(--el-color-warning-light-8)}.el-check-tag.el-check-tag--warning.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-disabled,.el-check-tag.el-check-tag--warning.is-disabled:hover{background-color:var(--el-color-warning-light-9)}.el-check-tag.el-check-tag--danger.is-checked{background-color:var(--el-color-danger-light-8);color:var(--el-color-danger)}.el-check-tag.el-check-tag--danger.is-checked:hover{background-color:var(--el-color-danger-light-7)}.el-check-tag.el-check-tag--danger.is-checked.is-disabled{background-color:var(--el-color-danger-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-checked.is-disabled:hover{background-color:var(--el-color-danger-light-8)}.el-check-tag.el-check-tag--danger.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-disabled,.el-check-tag.el-check-tag--danger.is-disabled:hover{background-color:var(--el-color-danger-light-9)}.el-check-tag.el-check-tag--error.is-checked{background-color:var(--el-color-error-light-8);color:var(--el-color-error)}.el-check-tag.el-check-tag--error.is-checked:hover{background-color:var(--el-color-error-light-7)}.el-check-tag.el-check-tag--error.is-checked.is-disabled{background-color:var(--el-color-error-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-checked.is-disabled:hover{background-color:var(--el-color-error-light-8)}.el-check-tag.el-check-tag--error.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-disabled,.el-check-tag.el-check-tag--error.is-disabled:hover{background-color:var(--el-color-error-light-9)}.el-check-tag.el-check-tag--info.is-checked{background-color:var(--el-color-info-light-8);color:var(--el-color-info)}.el-check-tag.el-check-tag--info.is-checked:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--info.is-checked.is-disabled{background-color:var(--el-color-info-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-checked.is-disabled:hover{background-color:var(--el-color-info-light-8)}.el-check-tag.el-check-tag--info.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-disabled,.el-check-tag.el-check-tag--info.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-checkbox-button{--el-checkbox-button-checked-bg-color:var(--el-color-primary);--el-checkbox-button-checked-text-color:var(--el-color-white);--el-checkbox-button-checked-border-color:var(--el-color-primary);display:inline-block;position:relative}.el-checkbox-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left-color:transparent;bord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15px;position:relative;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-checkbox-button__inner.is-round{padding:8px 15px}.el-checkbox-button__inner:hover{color:var(--el-color-primary)}.el-checkbox-button__inner [class*=el-icon-]{line-height:.9}.el-checkbox-button__inner [class*=el-icon-]+span{margin-left:5px}.el-checkbox-button__original{margin:0;opacity:0;outline:none;position:absolute;z-index:-1}.el-checkbox-button.is-checked .el-checkbox-button__inner{background-color:var(--el-checkbox-button-checked-bg-color);border-color:var(--el-checkbox-button-checked-border-color);box-shadow:-1px 0 0 0 var(--el-color-primary-light-7);color:var(--el-checkbox-button-checked-text-color)}.el-checkbox-button.is-checked:first-child .el-checkbox-button__inner{border-left-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button.is-disabled .el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large .el-checkbox-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-checkbox-button--large .el-checkbox-button__inner.is-round{padding:12px 19px}.el-checkbox-button--small .el-checkbox-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-checkbox-button--small .el-checkbox-button__inner.is-round{padding:5px 11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) .el-checkbox__original:not(:focus-visible){border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__input.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-checked-icon-color);content:"";display:block;height:2px;left:0;position:absolute;right:0;top:5px;transform:scale(.5)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:after{display:none}.el-checkbox__inner{background-color:var(--el-checkbox-bg-color);border:var(--el-checkbox-input-border);border-radius:var(--el-checkbox-border-radius);box-sizing:border-box;display:inline-block;height:var(--el-checkbox-input-height);position:relative;transition:border-color .25s cubic-bezier(.71,-.46,.29,1.46),background-color .25s cubic-bezier(.71,-.46,.29,1.46),outline .25s cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 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54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 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var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid 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var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker .el-picker-panel__content{margin:0}.el-date-range-picker__header{height:28px;position:relative;text-align:center}.el-date-range-picker__header [class*=arrow-left]{float:left}.el-date-range-picker__header [class*=arrow-right]{float:right}.el-date-range-picker__header div{font-size:16px;font-weight:500;margin-right:50px}.el-date-range-picker__content{box-sizing:border-box;float:left;margin:0;padding:16px;width:50%}.el-date-range-picker__content.is-left{border-right:1px solid var(--el-datepicker-inner-border-color)}.el-date-range-picker__content .el-date-range-picker__header div{margin-left:50px;margin-right:50px}.el-date-range-picker__editors-wrap{box-sizing:border-box;display:table-cell}.el-date-range-picker__editors-wrap.is-right{text-align:right}.el-date-range-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid var(--el-timepicker-inner-border-color,var(--el-border-color-light));box-sizing:border-box;height:36px;line-height:25px;padding:4px;text-align:right}.el-time-panel__btn{background-color:transparent;border:none;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:28px;margin:0 5px;outline:none;padding:0 5px}.el-time-panel__btn.confirm{color:var(--el-timepicker-active-color,var(--el-color-primary));font-weight:800}.el-descriptions{--el-descriptions-table-border:1px solid var(--el-border-color-lighter);--el-descriptions-item-bordered-label-background:var(--el-fill-color-light);box-sizing:border-box;color:var(--el-text-color-primary);font-size:var(--el-font-size-base)}.el-descriptions__header{align-items:center;display:flex;justify-content:space-between;margin-bottom:16px}.el-descriptions__title{color:var(--el-text-color-primary);font-size:16px;font-weight:700}.el-descriptions__body{background-color:var(--el-fill-color-blank)}.el-descriptions__body 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var(--el-menu-base-level-padding)}.horizontal-collapse-transition .el-sub-menu__title 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.el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small .el-radio__inner{height:12px;width:12px}.el-rate{--el-rate-height:20px;--el-rate-font-size:var(--el-font-size-base);--el-rate-icon-size:18px;--el-rate-icon-margin:6px;--el-rate-void-color:var(--el-border-color-darker);--el-rate-fill-color:#f7ba2a;--el-rate-disabled-void-color:var(--el-fill-color);--el-rate-text-color:var(--el-text-color-primary);align-items:center;display:inline-flex;height:32px}.el-rate:active,.el-rate:focus{outline:none}.el-rate__item{color:var(--el-rate-void-color);cursor:pointer;display:inline-block;font-size:0;line-height:normal;position:relative;vertical-align:middle}.el-rate .el-rate__icon{display:inline-block;font-size:var(--el-rate-icon-size);margin-right:var(--el-rate-icon-margin);position:relative;transition:var(--el-transition-duration)}.el-rate .el-rate__icon.hover{transform:scale(1.15)}.el-rate .el-rate__icon .path2{left:0;position:absolute;top:0}.el-rate .el-rate__icon.is-active{color:var(--el-rate-fill-color)}.el-rate__decimal{color:var(--el-rate-fill-color);display:inline-block;overflow:hidden}.el-rate__decimal,.el-rate__decimal--box{left:0;position:absolute;top:0}.el-rate__text{color:var(--el-rate-text-color);font-size:var(--el-rate-font-size);vertical-align:middle}.el-rate--large{height:40px}.el-rate--small{height:24px}.el-rate--small .el-rate__icon{font-size:14px}.el-rate.is-disabled .el-rate__item{color:var(--el-rate-disabled-void-color);cursor:auto}.el-result{--el-result-padding:40px 30px;--el-result-icon-font-size:64px;--el-result-title-font-size:20px;--el-result-title-margin-top:20px;--el-result-subtitle-margin-top:10px;--el-result-extra-margin-top:30px;align-items:center;box-sizing:border-box;display:flex;flex-direction:column;justify-content:center;padding:var(--el-result-padding);text-align:center}.el-result__icon svg{height:var(--el-result-icon-font-size);width:var(--el-result-icon-font-size)}.el-result__title{margin-top:var(--el-result-title-margin-top)}.el-result__title p{color:var(--el-text-color-primary);font-size:var(--el-result-title-font-size);line-height:1.3;margin:0}.el-result__subtitle{margin-top:var(--el-result-subtitle-margin-top)}.el-result__subtitle p{color:var(--el-text-color-regular);font-size:var(--el-font-size-base);line-height:1.3;margin:0}.el-result__extra{margin-top:var(--el-result-extra-margin-top)}.el-result .icon-primary{--el-result-color:var(--el-color-primary);color:var(--el-result-color)}.el-result .icon-success{--el-result-color:var(--el-color-success);color:var(--el-result-color)}.el-result .icon-warning{--el-result-color:var(--el-color-warning);color:var(--el-result-color)}.el-result .icon-danger{--el-result-color:var(--el-color-danger);color:var(--el-result-color)}.el-result .icon-error{--el-result-color:var(--el-color-error);color:var(--el-result-color)}.el-result 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.el-tag__close{color:var(--el-tag-text-color);flex-shrink:0}.el-tag .el-tag__close:hover{background-color:var(--el-tag-hover-color);color:var(--el-color-white)}.el-tag.el-tag--primary{--el-tag-text-color:var(--el-color-primary)}.el-tag.el-tag--success{--el-tag-text-color:var(--el-color-success)}.el-tag.el-tag--warning{--el-tag-text-color:var(--el-color-warning)}.el-tag.el-tag--danger{--el-tag-text-color:var(--el-color-danger)}.el-tag.el-tag--error{--el-tag-text-color:var(--el-color-error)}.el-tag.el-tag--info{--el-tag-text-color:var(--el-color-info)}.el-tag .el-icon{border-radius:50%;cursor:pointer;font-size:calc(var(--el-icon-size) - 2px);height:var(--el-icon-size);width:var(--el-icon-size)}.el-tag 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0;min-width:0}.time-select .el-picker-panel__content{margin:0;max-height:200px}.time-select-item{font-size:14px;line-height:20px;padding:8px 10px}.time-select-item.disabled{color:var(--el-datepicker-border-color);cursor:not-allowed}.time-select-item:hover{background-color:var(--el-fill-color-light);cursor:pointer;font-weight:700}.time-select .time-select-item.selected:not(.disabled){color:var(--el-color-primary);font-weight:700}.el-timeline-item{padding-bottom:20px;position:relative}.el-timeline-item__wrapper{padding-left:28px;position:relative;top:-3px}.el-timeline-item__tail{border-left:2px solid var(--el-timeline-node-color);height:100%;left:4px;position:absolute}.el-timeline-item .el-timeline-item__icon{color:var(--el-color-white);font-size:var(--el-font-size-small)}.el-timeline-item__node{align-items:center;background-color:var(--el-timeline-node-color);border-color:var(--el-timeline-node-color);border-radius:50%;box-sizing:border-box;display:flex;justify-content:center;position:absolute}.el-timeline-item__node--normal{height:var(--el-timeline-node-size-normal);left:-1px;width:var(--el-timeline-node-size-normal)}.el-timeline-item__node--large{height:var(--el-timeline-node-size-large);left:-2px;width:var(--el-timeline-node-size-large)}.el-timeline-item__node.is-hollow{background:var(--el-color-white);border-style:solid;border-width:2px}.el-timeline-item__node--primary{background-color:var(--el-color-primary);border-color:var(--el-color-primary)}.el-timeline-item__node--success{background-color:var(--el-color-success);border-color:var(--el-color-success)}.el-timeline-item__node--warning{background-color:var(--el-color-warning);border-color:var(--el-color-warning)}.el-timeline-item__node--danger{background-color:var(--el-color-danger);border-color:var(--el-color-danger)}.el-timeline-item__node--info{background-color:var(--el-color-info);border-color:var(--el-color-info)}.el-timeline-item__dot{align-items:center;display:flex;justify-content:center;position:absolute}.el-timeline-item__content{color:var(--el-text-color-primary)}.el-timeline-item__timestamp{color:var(--el-text-color-secondary);font-size:var(--el-font-size-small);line-height:1}.el-timeline-item__timestamp.is-top{margin-bottom:8px;padding-top:4px}.el-timeline-item__timestamp.is-bottom{margin-top:8px}.el-timeline{--el-timeline-node-size-normal:12px;--el-timeline-node-size-large:14px;--el-timeline-node-color:var(--el-border-color-light);font-size:var(--el-font-size-base);list-style:none;margin:0}.el-timeline 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onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="cover" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/drylab.webp 1x, https://static.igem.wiki/teams/5358/banner/drylab.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/drylab.webp" data-v-da2ffda7><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg" data-v-da2ffda7>Hardware</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10" data-v-da2ffda7><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[500px] md:mr-10" data-v-da2ffda7><h1 class="mt-0" data-v-da2ffda7>TOC</h1><ul data-v-da2ffda7><!--[--><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Demand Analysis</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Scheme Proposal</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Feedback</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Android Software</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Data Reception Verification</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Data Plotting Verification</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Improvement</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Array Splitting and Plotting Verification</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Summary</a></li><!--]--></ul></aside><div class="content" data-v-da2ffda7><p><!--[-->This year, the core objective of the BIT team is to develop <strong><!--[-->a initiative detection system for EDCs in aquatic environments based on E.coli biosensors.<!--]--></strong> Traditional detection methods, such as chromatography and immunoassays, require cumbersome procedures like sampling, pre-treatment, and laboratory examination, in addition to expensive laboratory equipment (e.g., chromatographs, microplate reader) and professional experimental techniques. These methods are time-consuming and costly.<!--]--></p><p><!--[-->As EDCs continue to threaten aquatic environments and the quality of drinking water for residents, it is crucial to achieve portable detection and rapid response to EDCs. To meet the requirement, our goal is to develop a low costing, highly integrated, compact, portable, and user-friendly hardware platform that can be combined with smartphone software to automate the cultivation of engineering bacteria and the analysis of fluorescence <strong><!--[-->detection<!--]--></strong>.<!--]--></p><p><!--[-->To ensure that the hardware device truly meets the needs of system construction, we have adopted a typical engineering and project design approach, namely: <strong><!--[-->demand analysis, scheme proposal, feasibility verification, feedback , system improvement, and goal achievement.<!--]--></strong> This process is an iterative one, and after several cycles, it ultimately meets all the needs and objectives.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/1.png 1x, https://static.igem.wiki/teams/5358/hardware/1.png 2x" src="https://static.igem.wiki/teams/5358/hardware/1.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 1 Hardware Design Flowchart</span><!--]--></p><h2 id="demand-analysis"><!--[-->Demand Analysis<!--]--></h2><p><!--[-->To ensure the stability and accuracy of <strong><!--[-->fluorescence detection<!--]--></strong>, it is necessary to standardize the detection process and use high sensitive detection units.<!--]--></p><p><!--[-->Standardization of the detection process requires a continuous and stable chemical and physical environment during the resuscitation of engineering bacteria and fluorescence detection. The standardization of the chemical environment is mainly ensured by the quantified operation of experimental steps (including control of sample volume and the number of engineering bacteria, control of the chemical composition and concentration of the culture medium), and the standardization of the physical environment is mainly ensured by the stability of light source, temperature, and humidity. Among them, light exposure problem has been solved by the sealed and light-proof nature of the device, and the humidity in the sealed environment is mainly determined by temperature. The standardization of the detection process mainly aims to achieve <strong><!--[-->constant temperature control<!--]--></strong> of the hardware device.<!--]--></p><p><!--[-->Since the concentration of <strong><!--[-->EDCs<!--]--></strong> in the detection samples is very low，which also refer to the low intensity of fluorescence, coupled with the portability requirements of the equipment, the detection method required must be high sensitive and can be integrated into small devices. Common chemical detection methods cannot meet the requirements, so adopting a more sensitive, portable, and efficient detection method is crucial for the realization of our POCT system.<!--]--></p><p><!--[-->In order to enable smartphone software to automate the control of hardware devices and visualize the detection results, the construction of hardware devices needs to consider the transmission of data between software and hardware.<!--]--></p><h2 id="scheme-proposal"><!--[-->Scheme Proposal<!--]--></h2><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/2.png 1x, https://static.igem.wiki/teams/5358/hardware/2.png 2x" src="https://static.igem.wiki/teams/5358/hardware/2.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 2 First Edition Design Scheme</span><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/3.png 1x, https://static.igem.wiki/teams/5358/hardware/3.png 2x" src="https://static.igem.wiki/teams/5358/hardware/3.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 3 Circuit Diagram</span><!--]--></p><p><!--[-->In the construction of the <strong><!--[-->temperature control module<!--]--></strong>, we employed an NTC thermistor as the <strong><!--[-->temperature sensor<!--]--></strong>, utilizing heating FILM and fan as the heat and cold sources respectively. To achieve <strong><!--[-->PID regulation<!--]--></strong> for <strong><!--[-->temperature sensing and control<!--]--></strong>, we used the PWM wave output from the <strong><!--[-->TB6612FNG motor drive<!--]--></strong> to adjust the power of the fan and heating FILM, thereby maintaining dynamic temperature stability.<!--]--></p><p><!--[-->For the selection of the detection scheme, we comprehensively considered the detection accuracy and the portability of the device, ultimately choosing the <strong><!--[-->fluorescence detection module<!--]--></strong>. We initially selected the AS7341 as <strong><!--[-->fluorescence sensor<!--]--></strong> <span>1</span>. Concurrently, to ensure consistent fluorescence generation, we redesigned and optimized the optical path.<!--]--></p><p><!--[-->After the marked fluorescence is excited by a blue LED, the fluorescence sensor collect and record real-time fluorescence signals, which can be visualized on the mobile software via a <strong><!--[-->communication module<!--]--></strong>.<!--]--></p><p><!--[-->For the communication module, we used the HC-05 Bluetooth module to connect the hardware system with the mobile software, enabling real-time monitoring of the hardware device&#39;s operating status and feedback of the detection results.<!--]--></p><p><!--[-->In the initial version of the scheme, we used an STM32 chip as the <strong><!--[-->microcontroller Unit(MCU)<!--]--></strong>, connecting temperature control module, fluorescence detection module, and Bluetooth communication module, ensuring the normal operation of the device through programming instructions.<!--]--></p><div class="table-fig"><!--[--><table><!--[--><thead><!--[--><tr><!--[--><th align="center"><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/4.png 1x, https://static.igem.wiki/teams/5358/hardware/4.png 2x" src="https://static.igem.wiki/teams/5358/hardware/4.png"><!--]--></th><th align="center"><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/5.png 1x, https://static.igem.wiki/teams/5358/hardware/5.png 2x" src="https://static.igem.wiki/teams/5358/hardware/5.png"><!--]--></th><!--]--></tr><!--]--></thead><tbody><!--[--><tr><!--[--><td align="center"><!--[-->Figure 5:  First Edition Implementation Scheme<!--]--></td><td align="center"><!--[-->Figure 6: First Edition Equipment Model Diagram<!--]--></td><!--]--></tr><!--]--></tbody><!--]--></table><!--]--></div><h3 id="feasibility-verification"><!--[-->Feasibility Verification<!--]--></h3><h3 id="temperature-stability-verification"><!--[-->Temperature Stability Verification<!--]--></h3><p><!--[-->To verify the constant temperature effect of the temperature control system, we designed a temperature stability test experiment. When the temperature first reached 37°C, the temperature was recorded every 10 seconds for an additional 60 seconds. From the results shown in Figure 6, it can be concluded that the absolute difference between the average temperature and the set temperature was no greater than 1.1°C, and the temperature curve consistently fluctuated around 37°C. This indicates that the temperature control system can achieve dynamic temperature regulation, capable of maintaining the temperature close to 37°C. However, this temperature control method generates relatively large temperature fluctuations.<!--]--></p><p><!--[--><span class="flex gap-4"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/6.png 1x, https://static.igem.wiki/teams/5358/hardware/6.png 2x" src="https://static.igem.wiki/teams/5358/hardware/6.png"> <img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/7.png 1x, https://static.igem.wiki/teams/5358/hardware/7.png 2x" src="https://static.igem.wiki/teams/5358/hardware/7.png"></span><!--]--></p><p><!--[--><span class="fig-title">Figure 7 Measurement Results of  Initial Scheme&#39;s Temperature Stability</span><!--]--></p><h3 id="gradient-fluoresce-in-sodium-fluorescence-detection-verification"><!--[-->Gradient Fluoresce in Sodium Fluorescence Detection Verification<!--]--></h3><p><!--[-->The AS7341 fluorescence sensor, by integrating filters of different wavelength bands, achieves the function of detecting light intensity (Lux) at specific wavelengths, which is equivalent to detecting the corresponding fluorescence intensity. To verify whether this sensor can correctly reflect the signal from the sample, we designed an experiment to explore the relationship between the concentration gradient of fluoresce in sodium and Lux. We prepared a series of fluoresce in sodium solutions with different gradients in a 2mL centrifuge tube for signal collection using the AS734. The results shown in Figure 8 indicate that the measured Lux values have a good linear correlation with the concentration gradient of fluoresce in sodium, demonstrating that our fluorescence detection module can accurately reflect the concentration of substances and is capable of conducting fluorescence detection.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/8.png 1x, https://static.igem.wiki/teams/5358/hardware/8.png 2x" src="https://static.igem.wiki/teams/5358/hardware/8.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 8 Initial Scheme&#39;s Fluorescence Verification Fitting Curve</span><!--]--></p><p><!--[-->The above feasibility verification demonstrates that the temperature control module and fluorescence detection module designed in the first version of the scheme can meet the design requirements and are feasible.<!--]--></p><h2 id="feedback"><!--[-->Feedback<!--]--></h2><p><!--[-->We engaged in in-depth discussions with the biology team, design team, and professional instructors regarding our hardware design, obtaining extremely valuable feedback. This feedback is instrumental in guiding our further improvements.<!--]--></p><p><!--[--><strong><!--[-->1.MCU:<!--]--></strong> The STM32 requires an external wireless communication module to implement Bluetooth functionality, which increases the complexity of the design.<!--]--></p><p><!--[--><strong><!--[-->2.Temperature Control Module:<!--]--></strong> Using the TB6612FNG to drive temperature control presents issues with heat dissipation and difficulties in achieving integration.<!--]--></p><p><!--[--><strong><!--[-->3.Detection Module:<!--]--></strong> The scheme of combining a fluorescence sensor with an optical path tends to have fluctuations in the excitation light source, and the optical path alignment is challenging and susceptible to environmental interference.<!--]--></p><p><!--[--><strong><!--[-->4.Biological Requirements:<!--]--></strong> The current design only allows for single detection, and frequent changes in the <strong><!--[-->reaction tube<!--]--></strong> can affect the stability of the detection environment, ultimately leading to inaccurate results.<!--]--></p><p><!--[-->In response to the issues raised and the suggestions from the instructors, we have made improvements based on the original design.<!--]--></p><p><!--[--><strong><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/9.png 1x, https://static.igem.wiki/teams/5358/hardware/9.png 2x" src="https://static.igem.wiki/teams/5358/hardware/9.png"><!--]--></strong><!--]--></p><p><!--[--><span class="fig-title">Figure 9 Improved Design Scheme</span><!--]--></p><p><!--[-->Firstly, we replaced the STM32F103C8T6 with the Arduino Nano ESP32 chip as the MCU. The ESP32 features built-in Bluetooth communication capabilities and a rich set of peripheral interfaces. It supports the CMake and Ninja build systems, which will greatly simplify the design complexity. Its widespread adoption globally will also make it more universal and laying a foundation for future teams looking to design similar devices. For temperature control and detection, we replaced the original scheme with the TCM1040 temperature control module and the FM-4A digital fluorescence detection module from LIDE Technology, respectively. Compared to the original scheme, these modules offer more integrated features, further enhancing the accuracy and stability of the platform.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/10.png 1x, https://static.igem.wiki/teams/5358/hardware/10.png 2x" src="https://static.igem.wiki/teams/5358/hardware/10.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 10 Improved Design Circuit Diagram</span><!--]--></p><p><!--[-->To meet the demand for high-throughput detection, we proposed two solutions: a microfluidic chip rotation detection platform and a linear slide rail detection platform. After modeling analysis and feasibility analysis, we ultimately chose the linear slide rail detection platform and designed a brand-new enclosure for it.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/11.png 1x, https://static.igem.wiki/teams/5358/hardware/11.png 2x" src="https://static.igem.wiki/teams/5358/hardware/11.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 11 Implementation Plan of the New Equipment</span><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/new.png 1x, https://static.igem.wiki/teams/5358/engineering-success/new.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/new.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 12 Model Diagram of the New Equipment</span><!--]--></p><h3 id="goal-achievement"><!--[-->Goal Achievement<!--]--></h3><h4 id="final-product"><!--[-->Final Product<!--]--></h4><p><!--[-->The final system is primarily divided into a <strong><!--[-->sampling area<!--]--></strong> and a <strong><!--[-->detection area.<!--]--></strong> In actual use, the freeze-dried engineering bacteria will be mixed with multiple samples to be tested in separate reaction chambers for cultivation, awaiting detection.Upon receiving operational commands from the software, the device rapidly heats the aluminum alloy temperature-controlled slot in <strong><!--[-->the sampling area<!--]--></strong> and maintains a constant temperature, while also providing real-time feedback of the temperature and the operational status of the system to the mobile software interface via Bluetooth. Concurrently, the fluorescence detection module in the detection area begins to sequentially detect the fluorescence signals of each reaction chamber along the slide rail and provides real-time feedback to the software&#39;s visual interface. When the engineering bacteria grow to a certain extent, the intensity of the fluorescence signal will reflect the degree of endocrine disruptor effects.<!--]--></p><h5 id="functional-testing"><!--[-->Functional Testing<!--]--></h5><p><!--[-->Temperature Control Capability Test<!--]--></p><p><!--[-->We conducted another temperature stability test on the latest version of the system. The results shown in Figure 12 indicate that the absolute difference between the average temperature and the set temperature is less than 0.3°C, suggesting that the stability of the improved temperature control system has been further enhanced. This can well meet practical requirements.<!--]--></p><p><!--[--><span class="flex gap-4"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/13.png 1x, https://static.igem.wiki/teams/5358/hardware/13.png 2x" src="https://static.igem.wiki/teams/5358/hardware/13.png"> <img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/14.png 1x, https://static.igem.wiki/teams/5358/hardware/14.png 2x" src="https://static.igem.wiki/teams/5358/hardware/14.png"></span><!--]--></p><p><!--[--><span class="fig-title">Figure 13 Measurement Results of Temperature Fluctuation</span><!--]--></p><h5 id="gradient-fluorescein-sodium-fluorescence-detection-verification"><!--[-->Gradient Fluorescein Sodium Fluorescence Detection Verification<!--]--></h5><p><!--[-->The fluorescence validation test of the latest equipment shows that the tested Relative Fluorescence Units (RFU) values have a good linear relationship with the concentration gradient of fluorescein sodium. This indicates that the new fluorescence detection module can effectively reflect the concentration of substances, meeting the usage requirements.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/15.png 1x, https://static.igem.wiki/teams/5358/hardware/15.png 2x" src="https://static.igem.wiki/teams/5358/hardware/15.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 14 Fluorescence Verification Experiment Fitting Curve</span><!--]--></p><h2 id="android-software"><!--[-->Android Software<!--]--></h2><p><!--[-->We have conducted functional testing on the already designed software. Initially, the software is imported onto an Android phone and the Bluetooth module is connected to check for a normal connection. The software is then opened to inspect whether the UI (User Interface) design is complete. Subsequently, the signals received from the module are used to test the graph plotting functionality to ensure it operates correctly, and the system is optimized based on the results.<!--]--></p><h2 id="data-reception-verification"><!--[-->Data Reception Verification<!--]--></h2><p><!--[-->Signals for initiation are transmitted from the mobile phone to the MCU via Bluetooth. As can be seen from the diagram, the hardware successfully starts up and returns a designated identifier.<!--]--></p><h2 id="data-plotting-verification"><!--[-->Data Plotting Verification<!--]--></h2><p><!--[-->During operation, the MCU sends data to the mobile phone via Bluetooth. The mobile phone then parses this data and plots graphs of time-temperature and time-fluorescence intensity. The diagrams indicate that the program has successfully implemented its functionalities.<!--]--></p><h2 id="improvement"><!--[-->Improvement<!--]--></h2><p><!--[-->Due to the addition of a linear slide rail for the movement of fluorescence detection in the second version of the hardware design, the signals obtained from the fluorescence detection have also changed. Therefore, the program is required to implement the ability to distinguish between the fluorescence intensity pulse peaks of different channels and to obtain their respective maximum values (theoretically, the fluorescence intensity at the bottom of the tube) for plotting.<!--]--></p><h2 id="array-splitting-and-plotting-verification"><!--[-->Array Splitting and Plotting Verification<!--]--></h2><p><!--[-->We can observe a set of data with multiple pulse peaks returned by the MCU. The software successfully splits this data into several different arrays and obtains the maximum value from each array for plotting.<!--]--></p><h2 id="summary"><!--[-->Summary<!--]--></h2><p><!--[-->The final product and its testing demonstrate that the proactive detection system for EDCs we have developed is compact, portable, and user-friendly. It effectively controls the standardization of cultivation conditions, enabling rapid screening and assessment of EDCs in wastewater, significantly reducing reliance on laboratory equipment and professional personnel. Additionally, the system&#39;s high-throughput operation allows for the simultaneous detection of multiple samples, thereby conducting sample testing more efficiently.<!--]--></p><p><!--[-->In designing the hardware, we employed engineering and project design approaches, taking into account target requirements and user feedback. Our instructor, Professor Li Xiaoqiong, recommended replacing the STM32 chip with the ESP32 chip, which will aid in enhancing the readability and versatility of the design. During testing and actual use, we collected feedback from operators and utilized a high-throughput platform, facilitating users to test multiple samples conveniently.<!--]--></p><p><!--[-->To facilitate user operation, we have added detailed user instructions in the App and will record demonstration videos for ease of use. At the same time, we have organized detailed system design blueprints and modeling files, as well as detailed component selection and manufacturing methods. You can contact us on the contribution page to obtain the relevant documents, thereby assisting other participants in replicating our device. Our main controller and major systems utilize common integrated units, while other parts are made using industrial processing and 3D printing techniques, making them easy to replicate for reference and implementation by other teams.<!--]--></p><p><!--[--><span class="text-2xl"><em><!--[-->References<!--]--></em></span><!--]--></p><div class="reference"><!--[--><ol><!--[--><li><!--[-->Wang Y, Chen H, Lin K, et al. Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device<span>j</span>. Nature Communications, 2024, 15(1):3279.<!--]--></li><!--]--></ol><!--]--></div></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a aria-current="page" href="/bit/hardware" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/hardware/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-1TDeI6fddl":11},null,"/hardware",1727868995313]</script>
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start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Human Practices<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li></ul><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-mobile px-10 flex lg:hidden"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, 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onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="cover" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/drylab.webp 1x, https://static.igem.wiki/teams/5358/banner/drylab.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/drylab.webp" data-v-da2ffda7><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg" data-v-da2ffda7>Hardware</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10" data-v-da2ffda7><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[500px] md:mr-10" data-v-da2ffda7><h1 class="mt-0" data-v-da2ffda7>TOC</h1><ul data-v-da2ffda7><!--[--><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Demand Analysis</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Scheme Proposal</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Feedback</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Android Software</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Data Reception Verification</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Data Plotting Verification</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Improvement</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Array Splitting and Plotting Verification</a></li><li class="my-4" data-v-da2ffda7><a class="cursor-pointer" data-v-da2ffda7>Summary</a></li><!--]--></ul></aside><div class="content" data-v-da2ffda7><p><!--[-->This year, the core objective of the BIT team is to develop <strong><!--[-->a initiative detection system for EDCs in aquatic environments based on E.coli biosensors.<!--]--></strong> Traditional detection methods, such as chromatography and immunoassays, require cumbersome procedures like sampling, pre-treatment, and laboratory examination, in addition to expensive laboratory equipment (e.g., chromatographs, microplate reader) and professional experimental techniques. These methods are time-consuming and costly.<!--]--></p><p><!--[-->As EDCs continue to threaten aquatic environments and the quality of drinking water for residents, it is crucial to achieve portable detection and rapid response to EDCs. To meet the requirement, our goal is to develop a low costing, highly integrated, compact, portable, and user-friendly hardware platform that can be combined with smartphone software to automate the cultivation of engineering bacteria and the analysis of fluorescence <strong><!--[-->detection<!--]--></strong>.<!--]--></p><p><!--[-->To ensure that the hardware device truly meets the needs of system construction, we have adopted a typical engineering and project design approach, namely: <strong><!--[-->demand analysis, scheme proposal, feasibility verification, feedback , system improvement, and goal achievement.<!--]--></strong> This process is an iterative one, and after several cycles, it ultimately meets all the needs and objectives.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/1.png 1x, https://static.igem.wiki/teams/5358/hardware/1.png 2x" src="https://static.igem.wiki/teams/5358/hardware/1.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 1 Hardware Design Flowchart</span><!--]--></p><h2 id="demand-analysis"><!--[-->Demand Analysis<!--]--></h2><p><!--[-->To ensure the stability and accuracy of <strong><!--[-->fluorescence detection<!--]--></strong>, it is necessary to standardize the detection process and use high sensitive detection units.<!--]--></p><p><!--[-->Standardization of the detection process requires a continuous and stable chemical and physical environment during the resuscitation of engineering bacteria and fluorescence detection. The standardization of the chemical environment is mainly ensured by the quantified operation of experimental steps (including control of sample volume and the number of engineering bacteria, control of the chemical composition and concentration of the culture medium), and the standardization of the physical environment is mainly ensured by the stability of light source, temperature, and humidity. Among them, light exposure problem has been solved by the sealed and light-proof nature of the device, and the humidity in the sealed environment is mainly determined by temperature. The standardization of the detection process mainly aims to achieve <strong><!--[-->constant temperature control<!--]--></strong> of the hardware device.<!--]--></p><p><!--[-->Since the concentration of <strong><!--[-->EDCs<!--]--></strong> in the detection samples is very low，which also refer to the low intensity of fluorescence, coupled with the portability requirements of the equipment, the detection method required must be high sensitive and can be integrated into small devices. Common chemical detection methods cannot meet the requirements, so adopting a more sensitive, portable, and efficient detection method is crucial for the realization of our POCT system.<!--]--></p><p><!--[-->In order to enable smartphone software to automate the control of hardware devices and visualize the detection results, the construction of hardware devices needs to consider the transmission of data between software and hardware.<!--]--></p><h2 id="scheme-proposal"><!--[-->Scheme Proposal<!--]--></h2><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/2.png 1x, https://static.igem.wiki/teams/5358/hardware/2.png 2x" src="https://static.igem.wiki/teams/5358/hardware/2.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 2 First Edition Design Scheme</span><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/3.png 1x, https://static.igem.wiki/teams/5358/hardware/3.png 2x" src="https://static.igem.wiki/teams/5358/hardware/3.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 3 Circuit Diagram</span><!--]--></p><p><!--[-->In the construction of the <strong><!--[-->temperature control module<!--]--></strong>, we employed an NTC thermistor as the <strong><!--[-->temperature sensor<!--]--></strong>, utilizing heating FILM and fan as the heat and cold sources respectively. To achieve <strong><!--[-->PID regulation<!--]--></strong> for <strong><!--[-->temperature sensing and control<!--]--></strong>, we used the PWM wave output from the <strong><!--[-->TB6612FNG motor drive<!--]--></strong> to adjust the power of the fan and heating FILM, thereby maintaining dynamic temperature stability.<!--]--></p><p><!--[-->For the selection of the detection scheme, we comprehensively considered the detection accuracy and the portability of the device, ultimately choosing the <strong><!--[-->fluorescence detection module<!--]--></strong>. We initially selected the AS7341 as <strong><!--[-->fluorescence sensor<!--]--></strong> <span>1</span>. Concurrently, to ensure consistent fluorescence generation, we redesigned and optimized the optical path.<!--]--></p><p><!--[-->After the marked fluorescence is excited by a blue LED, the fluorescence sensor collect and record real-time fluorescence signals, which can be visualized on the mobile software via a <strong><!--[-->communication module<!--]--></strong>.<!--]--></p><p><!--[-->For the communication module, we used the HC-05 Bluetooth module to connect the hardware system with the mobile software, enabling real-time monitoring of the hardware device&#39;s operating status and feedback of the detection results.<!--]--></p><p><!--[-->In the initial version of the scheme, we used an STM32 chip as the <strong><!--[-->microcontroller Unit(MCU)<!--]--></strong>, connecting temperature control module, fluorescence detection module, and Bluetooth communication module, ensuring the normal operation of the device through programming instructions.<!--]--></p><div class="table-fig"><!--[--><table><!--[--><thead><!--[--><tr><!--[--><th align="center"><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/4.png 1x, https://static.igem.wiki/teams/5358/hardware/4.png 2x" src="https://static.igem.wiki/teams/5358/hardware/4.png"><!--]--></th><th align="center"><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/5.png 1x, https://static.igem.wiki/teams/5358/hardware/5.png 2x" src="https://static.igem.wiki/teams/5358/hardware/5.png"><!--]--></th><!--]--></tr><!--]--></thead><tbody><!--[--><tr><!--[--><td align="center"><!--[-->Figure 5:  First Edition Implementation Scheme<!--]--></td><td align="center"><!--[-->Figure 6: First Edition Equipment Model Diagram<!--]--></td><!--]--></tr><!--]--></tbody><!--]--></table><!--]--></div><h3 id="feasibility-verification"><!--[-->Feasibility Verification<!--]--></h3><h3 id="temperature-stability-verification"><!--[-->Temperature Stability Verification<!--]--></h3><p><!--[-->To verify the constant temperature effect of the temperature control system, we designed a temperature stability test experiment. When the temperature first reached 37°C, the temperature was recorded every 10 seconds for an additional 60 seconds. From the results shown in Figure 6, it can be concluded that the absolute difference between the average temperature and the set temperature was no greater than 1.1°C, and the temperature curve consistently fluctuated around 37°C. This indicates that the temperature control system can achieve dynamic temperature regulation, capable of maintaining the temperature close to 37°C. However, this temperature control method generates relatively large temperature fluctuations.<!--]--></p><p><!--[--><span class="flex gap-4"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/6.png 1x, https://static.igem.wiki/teams/5358/hardware/6.png 2x" src="https://static.igem.wiki/teams/5358/hardware/6.png"> <img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/7.png 1x, https://static.igem.wiki/teams/5358/hardware/7.png 2x" src="https://static.igem.wiki/teams/5358/hardware/7.png"></span><!--]--></p><p><!--[--><span class="fig-title">Figure 7 Measurement Results of  Initial Scheme&#39;s Temperature Stability</span><!--]--></p><h3 id="gradient-fluoresce-in-sodium-fluorescence-detection-verification"><!--[-->Gradient Fluoresce in Sodium Fluorescence Detection Verification<!--]--></h3><p><!--[-->The AS7341 fluorescence sensor, by integrating filters of different wavelength bands, achieves the function of detecting light intensity (Lux) at specific wavelengths, which is equivalent to detecting the corresponding fluorescence intensity. To verify whether this sensor can correctly reflect the signal from the sample, we designed an experiment to explore the relationship between the concentration gradient of fluoresce in sodium and Lux. We prepared a series of fluoresce in sodium solutions with different gradients in a 2mL centrifuge tube for signal collection using the AS734. The results shown in Figure 8 indicate that the measured Lux values have a good linear correlation with the concentration gradient of fluoresce in sodium, demonstrating that our fluorescence detection module can accurately reflect the concentration of substances and is capable of conducting fluorescence detection.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/8.png 1x, https://static.igem.wiki/teams/5358/hardware/8.png 2x" src="https://static.igem.wiki/teams/5358/hardware/8.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 8 Initial Scheme&#39;s Fluorescence Verification Fitting Curve</span><!--]--></p><p><!--[-->The above feasibility verification demonstrates that the temperature control module and fluorescence detection module designed in the first version of the scheme can meet the design requirements and are feasible.<!--]--></p><h2 id="feedback"><!--[-->Feedback<!--]--></h2><p><!--[-->We engaged in in-depth discussions with the biology team, design team, and professional instructors regarding our hardware design, obtaining extremely valuable feedback. This feedback is instrumental in guiding our further improvements.<!--]--></p><p><!--[--><strong><!--[-->1.MCU:<!--]--></strong> The STM32 requires an external wireless communication module to implement Bluetooth functionality, which increases the complexity of the design.<!--]--></p><p><!--[--><strong><!--[-->2.Temperature Control Module:<!--]--></strong> Using the TB6612FNG to drive temperature control presents issues with heat dissipation and difficulties in achieving integration.<!--]--></p><p><!--[--><strong><!--[-->3.Detection Module:<!--]--></strong> The scheme of combining a fluorescence sensor with an optical path tends to have fluctuations in the excitation light source, and the optical path alignment is challenging and susceptible to environmental interference.<!--]--></p><p><!--[--><strong><!--[-->4.Biological Requirements:<!--]--></strong> The current design only allows for single detection, and frequent changes in the <strong><!--[-->reaction tube<!--]--></strong> can affect the stability of the detection environment, ultimately leading to inaccurate results.<!--]--></p><p><!--[-->In response to the issues raised and the suggestions from the instructors, we have made improvements based on the original design.<!--]--></p><p><!--[--><strong><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/9.png 1x, https://static.igem.wiki/teams/5358/hardware/9.png 2x" src="https://static.igem.wiki/teams/5358/hardware/9.png"><!--]--></strong><!--]--></p><p><!--[--><span class="fig-title">Figure 9 Improved Design Scheme</span><!--]--></p><p><!--[-->Firstly, we replaced the STM32F103C8T6 with the Arduino Nano ESP32 chip as the MCU. The ESP32 features built-in Bluetooth communication capabilities and a rich set of peripheral interfaces. It supports the CMake and Ninja build systems, which will greatly simplify the design complexity. Its widespread adoption globally will also make it more universal and laying a foundation for future teams looking to design similar devices. For temperature control and detection, we replaced the original scheme with the TCM1040 temperature control module and the FM-4A digital fluorescence detection module from LIDE Technology, respectively. Compared to the original scheme, these modules offer more integrated features, further enhancing the accuracy and stability of the platform.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/10.png 1x, https://static.igem.wiki/teams/5358/hardware/10.png 2x" src="https://static.igem.wiki/teams/5358/hardware/10.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 10 Improved Design Circuit Diagram</span><!--]--></p><p><!--[-->To meet the demand for high-throughput detection, we proposed two solutions: a microfluidic chip rotation detection platform and a linear slide rail detection platform. After modeling analysis and feasibility analysis, we ultimately chose the linear slide rail detection platform and designed a brand-new enclosure for it.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/11.png 1x, https://static.igem.wiki/teams/5358/hardware/11.png 2x" src="https://static.igem.wiki/teams/5358/hardware/11.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 11 Implementation Plan of the New Equipment</span><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/engineering-success/new.png 1x, https://static.igem.wiki/teams/5358/engineering-success/new.png 2x" src="https://static.igem.wiki/teams/5358/engineering-success/new.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 12 Model Diagram of the New Equipment</span><!--]--></p><h3 id="goal-achievement"><!--[-->Goal Achievement<!--]--></h3><h4 id="final-product"><!--[-->Final Product<!--]--></h4><p><!--[-->The final system is primarily divided into a <strong><!--[-->sampling area<!--]--></strong> and a <strong><!--[-->detection area.<!--]--></strong> In actual use, the freeze-dried engineering bacteria will be mixed with multiple samples to be tested in separate reaction chambers for cultivation, awaiting detection.Upon receiving operational commands from the software, the device rapidly heats the aluminum alloy temperature-controlled slot in <strong><!--[-->the sampling area<!--]--></strong> and maintains a constant temperature, while also providing real-time feedback of the temperature and the operational status of the system to the mobile software interface via Bluetooth. Concurrently, the fluorescence detection module in the detection area begins to sequentially detect the fluorescence signals of each reaction chamber along the slide rail and provides real-time feedback to the software&#39;s visual interface. When the engineering bacteria grow to a certain extent, the intensity of the fluorescence signal will reflect the degree of endocrine disruptor effects.<!--]--></p><h5 id="functional-testing"><!--[-->Functional Testing<!--]--></h5><p><!--[-->Temperature Control Capability Test<!--]--></p><p><!--[-->We conducted another temperature stability test on the latest version of the system. The results shown in Figure 12 indicate that the absolute difference between the average temperature and the set temperature is less than 0.3°C, suggesting that the stability of the improved temperature control system has been further enhanced. This can well meet practical requirements.<!--]--></p><p><!--[--><span class="flex gap-4"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/13.png 1x, https://static.igem.wiki/teams/5358/hardware/13.png 2x" src="https://static.igem.wiki/teams/5358/hardware/13.png"> <img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/14.png 1x, https://static.igem.wiki/teams/5358/hardware/14.png 2x" src="https://static.igem.wiki/teams/5358/hardware/14.png"></span><!--]--></p><p><!--[--><span class="fig-title">Figure 13 Measurement Results of Temperature Fluctuation</span><!--]--></p><h5 id="gradient-fluorescein-sodium-fluorescence-detection-verification"><!--[-->Gradient Fluorescein Sodium Fluorescence Detection Verification<!--]--></h5><p><!--[-->The fluorescence validation test of the latest equipment shows that the tested Relative Fluorescence Units (RFU) values have a good linear relationship with the concentration gradient of fluorescein sodium. This indicates that the new fluorescence detection module can effectively reflect the concentration of substances, meeting the usage requirements.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/hardware/15.png 1x, https://static.igem.wiki/teams/5358/hardware/15.png 2x" src="https://static.igem.wiki/teams/5358/hardware/15.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 14 Fluorescence Verification Experiment Fitting Curve</span><!--]--></p><h2 id="android-software"><!--[-->Android Software<!--]--></h2><p><!--[-->We have conducted functional testing on the already designed software. Initially, the software is imported onto an Android phone and the Bluetooth module is connected to check for a normal connection. The software is then opened to inspect whether the UI (User Interface) design is complete. Subsequently, the signals received from the module are used to test the graph plotting functionality to ensure it operates correctly, and the system is optimized based on the results.<!--]--></p><h2 id="data-reception-verification"><!--[-->Data Reception Verification<!--]--></h2><p><!--[-->Signals for initiation are transmitted from the mobile phone to the MCU via Bluetooth. As can be seen from the diagram, the hardware successfully starts up and returns a designated identifier.<!--]--></p><h2 id="data-plotting-verification"><!--[-->Data Plotting Verification<!--]--></h2><p><!--[-->During operation, the MCU sends data to the mobile phone via Bluetooth. The mobile phone then parses this data and plots graphs of time-temperature and time-fluorescence intensity. The diagrams indicate that the program has successfully implemented its functionalities.<!--]--></p><h2 id="improvement"><!--[-->Improvement<!--]--></h2><p><!--[-->Due to the addition of a linear slide rail for the movement of fluorescence detection in the second version of the hardware design, the signals obtained from the fluorescence detection have also changed. Therefore, the program is required to implement the ability to distinguish between the fluorescence intensity pulse peaks of different channels and to obtain their respective maximum values (theoretically, the fluorescence intensity at the bottom of the tube) for plotting.<!--]--></p><h2 id="array-splitting-and-plotting-verification"><!--[-->Array Splitting and Plotting Verification<!--]--></h2><p><!--[-->We can observe a set of data with multiple pulse peaks returned by the MCU. The software successfully splits this data into several different arrays and obtains the maximum value from each array for plotting.<!--]--></p><h2 id="summary"><!--[-->Summary<!--]--></h2><p><!--[-->The final product and its testing demonstrate that the proactive detection system for EDCs we have developed is compact, portable, and user-friendly. It effectively controls the standardization of cultivation conditions, enabling rapid screening and assessment of EDCs in wastewater, significantly reducing reliance on laboratory equipment and professional personnel. Additionally, the system&#39;s high-throughput operation allows for the simultaneous detection of multiple samples, thereby conducting sample testing more efficiently.<!--]--></p><p><!--[-->In designing the hardware, we employed engineering and project design approaches, taking into account target requirements and user feedback. Our instructor, Professor Li Xiaoqiong, recommended replacing the STM32 chip with the ESP32 chip, which will aid in enhancing the readability and versatility of the design. During testing and actual use, we collected feedback from operators and utilized a high-throughput platform, facilitating users to test multiple samples conveniently.<!--]--></p><p><!--[-->To facilitate user operation, we have added detailed user instructions in the App and will record demonstration videos for ease of use. At the same time, we have organized detailed system design blueprints and modeling files, as well as detailed component selection and manufacturing methods. You can contact us on the contribution page to obtain the relevant documents, thereby assisting other participants in replicating our device. Our main controller and major systems utilize common integrated units, while other parts are made using industrial processing and 3D printing techniques, making them easy to replicate for reference and implementation by other teams.<!--]--></p><p><!--[--><span class="text-2xl"><em><!--[-->References<!--]--></em></span><!--]--></p><div class="reference"><!--[--><ol><!--[--><li><!--[-->Wang Y, Chen H, Lin K, et al. Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device<span>j</span>. Nature Communications, 2024, 15(1):3279.<!--]--></li><!--]--></ol><!--]--></div></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a aria-current="page" href="/bit/hardware" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/hardware/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-1TDeI6fddl":11},null,"/hardware",1727871092354]</script>
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+++ b/.output/public/human-practices/_payload.json
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-[{"data":1,"prerenderedAt":296},["Reactive",2],{"content-query-dSb1ehdCUe":3},{"_path":4,"_dir":5,"_draft":6,"_partial":6,"_locale":5,"title":7,"description":5,"body":8,"_type":290,"_id":291,"_source":292,"_file":293,"_stem":294,"_extension":295},"/hp-ihp","",false,"Integrated Human Practice",{"type":9,"children":10,"toc":267},"root",[11,20,26,34,39,45,50,55,60,66,71,77,82,89,96,101,106,111,116,122,128,135,140,145,150,155,160,166,171,177,184,189,194,200,207,212,217,226,232,239,244,249,255,260],{"type":12,"tag":13,"props":14,"children":16},"element","h2",{"id":15},"overview",[17],{"type":18,"value":19},"text","Overview",{"type":12,"tag":21,"props":22,"children":23},"p",{},[24],{"type":18,"value":25},"The core of HP is to present project designs and products to the community and stakeholders, get feedback, integrate feedback into the project, and constantly adjust and modify the project according to the guidance of the feedback. This year, we proposed the \"BRIEF\" periodic iteration method to complete IHP. The aim is to ensure that projects meet practical needs, continuously improve project performance through feedback, meet the needs of stakeholders, and contribute to society in order to build projects that are good and responsible for the world.",{"type":12,"tag":21,"props":27,"children":28},{},[29],{"type":12,"tag":30,"props":31,"children":33},"img",{"alt":5,"src":32},"https://static.igem.wiki/teams/5358/ihp/brief.jpg",[],{"type":12,"tag":21,"props":35,"children":36},{},[37],{"type":18,"value":38},"The \"BRIEF\" concept consists of five phases of IHP implementation, They are Brainstorming, Researching, Insight Integrating, Executing and Adjusting, and Feedback Recollecting five aspects. Among them, Executing and Adjusting the project is a bridge connecting Brainstorming, Researching, Insight Integrating and Feedback Recollecting. Throughout IHP, we build a cycle of finding meaning, reaching out, and connecting our projects to the world.",{"type":12,"tag":13,"props":40,"children":42},{"id":41},"brainstorming",[43],{"type":18,"value":44},"Brainstorming",{"type":12,"tag":21,"props":46,"children":47},{},[48],{"type":18,"value":49},"Our team is focusing on detecting new pollutants this year. At the beginning of the project, we carried out extensive research on new pollutants, and focused on endocrine disruptors, microplastics, antibiotics, persistent pollutants four, because of their greater harm and detection methods are expensive and difficult to operate.",{"type":12,"tag":21,"props":51,"children":52},{},[53],{"type":18,"value":54},"Since then, endocrine disruptors have been screened according to the difficulty of detection, the harm to the environment and the significance of positive results. In the traditional evaluation system of endocrine disruptors, most of the main methods are to detect their binding with hormone receptors. However, due to the extensive and complex toxic effects of endocrine disruptors and their metabolites on organisms, the traditional detection methods are obviously inadequate.",{"type":12,"tag":21,"props":56,"children":57},{},[58],{"type":18,"value":59},"The result of our brainstorming is to build a gate-based multi-toxicological screening sensor that can simultaneously detect endocrine disrupting effects and DNA damage effects in factory and enterprise products, or unknown samples in the environment.",{"type":12,"tag":13,"props":61,"children":63},{"id":62},"researching",[64],{"type":18,"value":65},"Researching",{"type":12,"tag":21,"props":67,"children":68},{},[69],{"type":18,"value":70},"After determining the study of endocrine disruptors, we screened out methods that could detect endocrine disruptors through literature research and conducted feasibility analysis. Finally, a method was established to detect endocrine disruptors by constructing hormone analogues sensitive peptides and coupled with Escherichia coli SOS oxidative damage response system and hrp amplifier for detecting DNA damage effects to construct a gated multi-toxicological effects screening sensor design. In addition, we investigated the contained peptide sequence and found that the number of bases was too large, which would cause a heavy burden on Escherichia coli. Therefore, the components for detecting the two effects were divided into two bacteria, and the LuxI/LuxR system was used for communication. Biosensors use fluorescent protein reports.\nThis is the initial design of our project.",{"type":12,"tag":13,"props":72,"children":74},{"id":73},"insight-integrating",[75],{"type":18,"value":76},"Insight Integrating",{"type":12,"tag":21,"props":78,"children":79},{},[80],{"type":18,"value":81},"In order to identify the social needs of our project with stakeholders, we contacted environmental testing companies, government departments, factories and wastewater treatment plants to ask about the needs and status of pollutant monitoring. In addition, in order to enhance the professional and scientific nature of the project, we also consult with researchers in related fields to adjust our research direction and research content.",{"type":12,"tag":83,"props":84,"children":86},"h3",{"id":85},"government-department",[87],{"type":18,"value":88},"Government Department",{"type":12,"tag":90,"props":91,"children":93},"h4",{"id":92},"bureau-of-ecology-environment-and-environmental-protection",[94],{"type":18,"value":95},"Bureau of Ecology Environment and Environmental Protection",{"type":12,"tag":21,"props":97,"children":98},{},[99],{"type":18,"value":100},"On May 23, 2024, our team members approached Ms. Luo, an official of Chongqing Ecological Environment and Environmental Protection Bureau, to consult the social needs of our project.\nFirst of all, we consulted the definition method, detection and registration process and control measures of endocrine disruptors. Luo pointed out that after confirming that a substance is polluting, the government departments will commission professional technical institutions to conduct research and investigation every year, and if the result is ultra-ultra-trace, relatively safe, no further measures will be taken. But if the concentration is high, the government will take measures and test the water quality of the test site or its source to track its possible source. In the analysis of new pollutants, including endocrine disruptors, government departments will conduct toxicological tests on chemical substances based on cutting-edge international research to determine that a substance may be harmful to the human body. This is a very important part of the testing process, because the government departments will concentrate the samples, which will lead to a variety of substances will be detected, and only when these substances are associated with toxicological effects and confirmed to be harmful to the human body, it is necessary to define endocrine disruptors and monitor. In terms of control, the government has corresponding emission standards for well-known endocrine disruptors; For new pollutants such as endocrine disruptors detected, the government will continue to confirm whether they belong to the emission substances already on the control list. For the situation where the pollutant index exceeds the standard, the government department will require the enterprise to take corresponding treatment measures and implement management measures such as regular review in the follow-up to supervise the rectification results.",{"type":12,"tag":21,"props":102,"children":103},{},[104],{"type":18,"value":105},"As for endocrine disruptors, Luo pointed out that in some studies, all organic compounds were classified as endocrine disruptors under investigation, which is not accurate; The current approach is to study each emerging substance independently and then classify it. The test site of endocrine disruptors is generally in other important water bodies such as drinking water sources, livestock and poultry farms and chemical plants and other emission sources.",{"type":12,"tag":21,"props":107,"children":108},{},[109],{"type":18,"value":110},"Secondly, we proposed a method to determine whether the water quality contains endocrine disruptors by using the biosensor constructed by the engineering bacteria that specifically recognizes endocrine disruptors. Luo first confirmed that in the environmental field, the emergency equipment launched abroad in the past few years does include microbial sensors and fluorescence detection technology to deal with sudden emergency environmental accidents. For endocrine disruptors, the government does not include them in routine testing tasks, but they are regularly included in testing efforts. The government prefers to use more accurate tests to detect endocrine disruptors. Therefore, from this point of view, improving the accuracy and sensitivity of the detection is also very beneficial and important.",{"type":12,"tag":21,"props":112,"children":113},{},[114],{"type":18,"value":115},"In short, Luo affirmed our project's detection method of using highly sensitive and specific biosensors for endocrine disruptors in the environment, which is very necessary and beneficial for government departments to detect endocrine disruptors, and our project is in line with the needs of government departments.",{"type":12,"tag":83,"props":117,"children":119},{"id":118},"environmental-testing-company",[120],{"type":18,"value":121},"Environmental Testing Company",{"type":12,"tag":90,"props":123,"children":125},{"id":124},"shanghai-environmental-monitoring-technology-co-ltd",[126],{"type":18,"value":127},"Shanghai Environmental Monitoring Technology Co., Ltd",{"type":12,"tag":21,"props":129,"children":130},{},[131],{"type":12,"tag":30,"props":132,"children":134},{"alt":5,"src":133},"https://static.igem.wiki/teams/5358/ihp/guo.png",[],{"type":12,"tag":21,"props":136,"children":137},{},[138],{"type":18,"value":139},"In order to continue to understand the definition method and testing registration process of new pollutants, on August 1, 2024, we found Mr. Guo, chief engineer of Shanghai Environmental Monitoring Technology Co., Ltd. for consultation.",{"type":12,"tag":21,"props":141,"children":142},{},[143],{"type":18,"value":144},"Guo pointed out that the determination of pollutants is first based on those already found by the country, and their emissions often exceed the prescribed standards, while classification and the formulation of standards are needed. The study of these pollutants needs to determine whether they have harmful effects on the human body through effect analysis, toxicology analysis and process analysis. Secondly, the use of foreign literature research combined with the actual situation in China to develop a standardized detection process to find endocrine disruptors. The definition of most new pollutants, especially endocrine disruptors, is that they are first found to be harmful to human health, and then they are analyzed in detail.",{"type":12,"tag":21,"props":146,"children":147},{},[148],{"type":18,"value":149},"For the analysis standards of new pollutants such as endocrine disruptors, Guo mentioned that with the development of industry, some new substances will be continuously studied, but whether it is harmful to the human body needs to be determined through a scientific judgment process, and if it is found to be harmful to the human body, it needs to be immediately included in the supervision. According to the requirements of the state, Shanghai has put the new pollutants to be studied into the supervision. Both testing organizations and industry systems are constantly developing methods to detect new pollutants, including endocrine disruptors, and current detection instruments are sophisticated and expensive.",{"type":12,"tag":21,"props":151,"children":152},{},[153],{"type":18,"value":154},"In the subsequent improvement process, the hardware device has undergone a simplified design to achieve the above design goals of portability and miniaturization.",{"type":12,"tag":21,"props":156,"children":157},{},[158],{"type":18,"value":159},"In the communication with Luo and Guo, they both affirmed our thinking and importance of detecting new pollutants such as endocrine pollutants through toxicological effects.",{"type":12,"tag":83,"props":161,"children":163},{"id":162},"factory-and-enterprise",[164],{"type":18,"value":165},"Factory and Enterprise",{"type":12,"tag":21,"props":167,"children":168},{},[169],{"type":18,"value":170},"Our next step is to understand the current status of the detection and analysis of endocrine disruptors from the perspective of relevant units and companies, as well as the specific needs currently existing, and ask whether there are applicable scenarios for the project. In accordance with Luo's previous guidance, we found a sewage plant and a water plant in Shanghai on August 1, 2024.",{"type":12,"tag":90,"props":172,"children":174},{"id":173},"shanghai-taihe-wastewater-treatment-plant",[175],{"type":18,"value":176},"Shanghai Taihe Wastewater Treatment Plant",{"type":12,"tag":21,"props":178,"children":179},{},[180],{"type":12,"tag":30,"props":181,"children":183},{"alt":5,"src":182},"https://static.igem.wiki/teams/5358/ihp/liu.png",[],{"type":12,"tag":21,"props":185,"children":186},{},[187],{"type":18,"value":188},"We have found Mr. Liu, director of Shanghai Taihe Sewage Plant. His reply to the above question is that the main pollutants under supervision of the sewage plant are Cod, Bod, ammonia, nitrogen and phosphorus. However, the pollutants under supervision of the sewage plant are to send water samples to testing institutions for testing, and domestic sewage involves few substances with toxicological properties.",{"type":12,"tag":21,"props":190,"children":191},{},[192],{"type":18,"value":193},"For our project, Mr. Liu said that due to the above reasons, the emergence of relevant technologies is temporarily lacking. Therefore, we should reflect on the use cases of our own projects and focus on providing services to government departments and environmental monitoring agencies.",{"type":12,"tag":90,"props":195,"children":197},{"id":196},"shanghai-nanhui-water-plant",[198],{"type":18,"value":199},"Shanghai Nanhui Water Plant",{"type":12,"tag":21,"props":201,"children":202},{},[203],{"type":12,"tag":30,"props":204,"children":206},{"alt":5,"src":205},"https://static.igem.wiki/teams/5358/ihp/ma.png",[],{"type":12,"tag":21,"props":208,"children":209},{},[210],{"type":18,"value":211},"In the drinking water industry, we found Mr. Ma, director of Shanghai Nanhui Water Plant, for consultation. In response to the above problems, the director pointed out that the detection of new pollutants in the drinking water industry, including endocrine disruptors, must use more expensive instruments, and the detection requirements and operations are more complicated; There is a need for emergency detection of source water for the use of biosensors. In view of our team's project, the factory director put forward that it can be applied in the rapid emergency monitoring of the above source water, and put forward the suggestions and technical requirements of our products for both qualitative and quantitative testing of drinking water source water.",{"type":12,"tag":21,"props":213,"children":214},{},[215],{"type":18,"value":216},"Therefore, for factories and enterprises, our project has a demand in the rapid emergency monitoring of drinking water source water, and we need to design a gate-based multi-toxicological effect screening sensor that can carry out qualitative and quantitative analysis on the basis of the project and is equipped with portable detection equipment.",{"type":12,"tag":21,"props":218,"children":219},{},[220,222],{"type":18,"value":221},"In short, the need for endocrine disruptor testing that we investigated is actually confirmed. At present, the detection of new pollutants including endocrine disruptors is more passive, and the method is more heavy on toxicological analysis, and has high cost and cumbersome operation. Therefore, there is a real need for a highly sensitive and specific detection of endocrine disruption and DNA damage in unknown samples, two important toxicological effects, and the hardware of such sensors must be portable and miniaturized.",{"type":12,"tag":30,"props":223,"children":225},{"alt":5,"src":224},"https://static.igem.wiki/teams/5358/ihp/projectpropose.png",[],{"type":12,"tag":83,"props":227,"children":229},{"id":228},"professional-research-personnel",[230],{"type":18,"value":231},"Professional research personnel",{"type":12,"tag":21,"props":233,"children":234},{},[235],{"type":12,"tag":30,"props":236,"children":238},{"alt":5,"src":237},"https://static.igem.wiki/teams/5358/ihp/yang.jpg",[],{"type":12,"tag":21,"props":240,"children":241},{},[242],{"type":18,"value":243},"We had an in-depth discussion on Modeling with Professor Yang Guanghui of China Agricultural University. He first gave a positive opinion of our idea. As the latest achievement in the field of bioinformatics, Alphafold3 has a high degree of confidence in the prediction of protein structure function, which can effectively guide our research in synthetic biology. But he also points out that the number and length of hydrogen bonds alone cannot accurately measure how folded a protein domain is. The main force in protein folding is hydrophobic interaction. However, there are no effective means to quantitatively measure the degree of protein folding and the strength of hydrophobic interaction. He encouraged us to work in this direction, hoping that we would be able to measure protein folding from other angles.",{"type":12,"tag":21,"props":245,"children":246},{},[247],{"type":18,"value":248},"Inspired by this, and after subsequent investigation, we will use Chimera1.13 software to analyze the hydrophobic interaction of the protein conformation obtained by molecular dynamics simulation (MDS), and measure the hydrophobic interaction intensity by comparing the hydrophobic potential around the recAN end and the C-end, so as to compare the degree of folding of this domain in the protein",{"type":12,"tag":13,"props":250,"children":252},{"id":251},"feedback-recollecting",[253],{"type":18,"value":254},"Feedback Recollecting",{"type":12,"tag":21,"props":256,"children":257},{},[258],{"type":18,"value":259},"We plan to bring the prototype design to endocrine disruptor experts and previous stakeholders, and continue to receive their comments and feedback to continue to adapt our project; At the same time, we will also find new innovation points and needs from the feedback, so as to launch a new round of \"BRIEF\" cycle in the future and complete the closed loop.",{"type":12,"tag":21,"props":261,"children":262},{},[263],{"type":12,"tag":30,"props":264,"children":266},{"alt":5,"src":265},"https://static.igem.wiki/teams/5358/ihp/executingandadjusting.png",[],{"title":5,"searchDepth":268,"depth":269,"links":270},1,3,[271,273,274,275,289],{"id":15,"depth":272,"text":19},2,{"id":41,"depth":272,"text":44},{"id":62,"depth":272,"text":65},{"id":73,"depth":272,"text":76,"children":276},[277,281,284,288],{"id":85,"depth":269,"text":88,"children":278},[279],{"id":92,"depth":280,"text":95},4,{"id":118,"depth":269,"text":121,"children":282},[283],{"id":124,"depth":280,"text":127},{"id":162,"depth":269,"text":165,"children":285},[286,287],{"id":173,"depth":280,"text":176},{"id":196,"depth":280,"text":199},{"id":228,"depth":269,"text":231},{"id":251,"depth":272,"text":254},"markdown","content:hp-ihp.md","content","hp-ihp.md","hp-ihp","md",1727868995423]
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+[{"data":1,"prerenderedAt":296},["Reactive",2],{"content-query-dSb1ehdCUe":3},{"_path":4,"_dir":5,"_draft":6,"_partial":6,"_locale":5,"title":7,"description":5,"body":8,"_type":290,"_id":291,"_source":292,"_file":293,"_stem":294,"_extension":295},"/hp-ihp","",false,"Integrated Human Practice",{"type":9,"children":10,"toc":267},"root",[11,20,26,34,39,45,50,55,60,66,71,77,82,89,96,101,106,111,116,122,128,135,140,145,150,155,160,166,171,177,184,189,194,200,207,212,217,226,232,239,244,249,255,260],{"type":12,"tag":13,"props":14,"children":16},"element","h2",{"id":15},"overview",[17],{"type":18,"value":19},"text","Overview",{"type":12,"tag":21,"props":22,"children":23},"p",{},[24],{"type":18,"value":25},"The core of HP is to present project designs and products to the community and stakeholders, get feedback, integrate feedback into the project, and constantly adjust and modify the project according to the guidance of the feedback. This year, we proposed the \"BRIEF\" periodic iteration method to complete IHP. The aim is to ensure that projects meet practical needs, continuously improve project performance through feedback, meet the needs of stakeholders, and contribute to society in order to build projects that are good and responsible for the world.",{"type":12,"tag":21,"props":27,"children":28},{},[29],{"type":12,"tag":30,"props":31,"children":33},"img",{"alt":5,"src":32},"https://static.igem.wiki/teams/5358/ihp/brief.jpg",[],{"type":12,"tag":21,"props":35,"children":36},{},[37],{"type":18,"value":38},"The \"BRIEF\" concept consists of five phases of IHP implementation, They are Brainstorming, Researching, Insight Integrating, Executing and Adjusting, and Feedback Recollecting five aspects. Among them, Executing and Adjusting the project is a bridge connecting Brainstorming, Researching, Insight Integrating and Feedback Recollecting. Throughout IHP, we build a cycle of finding meaning, reaching out, and connecting our projects to the world.",{"type":12,"tag":13,"props":40,"children":42},{"id":41},"brainstorming",[43],{"type":18,"value":44},"Brainstorming",{"type":12,"tag":21,"props":46,"children":47},{},[48],{"type":18,"value":49},"Our team is focusing on detecting new pollutants this year. At the beginning of the project, we carried out extensive research on new pollutants, and focused on endocrine disruptors, microplastics, antibiotics, persistent pollutants four, because of their greater harm and detection methods are expensive and difficult to operate.",{"type":12,"tag":21,"props":51,"children":52},{},[53],{"type":18,"value":54},"Since then, endocrine disruptors have been screened according to the difficulty of detection, the harm to the environment and the significance of positive results. In the traditional evaluation system of endocrine disruptors, most of the main methods are to detect their binding with hormone receptors. However, due to the extensive and complex toxic effects of endocrine disruptors and their metabolites on organisms, the traditional detection methods are obviously inadequate.",{"type":12,"tag":21,"props":56,"children":57},{},[58],{"type":18,"value":59},"The result of our brainstorming is to build a gate-based multi-toxicological screening sensor that can simultaneously detect endocrine disrupting effects and DNA damage effects in factory and enterprise products, or unknown samples in the environment.",{"type":12,"tag":13,"props":61,"children":63},{"id":62},"researching",[64],{"type":18,"value":65},"Researching",{"type":12,"tag":21,"props":67,"children":68},{},[69],{"type":18,"value":70},"After determining the study of endocrine disruptors, we screened out methods that could detect endocrine disruptors through literature research and conducted feasibility analysis. Finally, a method was established to detect endocrine disruptors by constructing hormone analogues sensitive peptides and coupled with Escherichia coli SOS oxidative damage response system and hrp amplifier for detecting DNA damage effects to construct a gated multi-toxicological effects screening sensor design. In addition, we investigated the contained peptide sequence and found that the number of bases was too large, which would cause a heavy burden on Escherichia coli. Therefore, the components for detecting the two effects were divided into two bacteria, and the LuxI/LuxR system was used for communication. Biosensors use fluorescent protein reports.\nThis is the initial design of our project.",{"type":12,"tag":13,"props":72,"children":74},{"id":73},"insight-integrating",[75],{"type":18,"value":76},"Insight Integrating",{"type":12,"tag":21,"props":78,"children":79},{},[80],{"type":18,"value":81},"In order to identify the social needs of our project with stakeholders, we contacted environmental testing companies, government departments, factories and wastewater treatment plants to ask about the needs and status of pollutant monitoring. In addition, in order to enhance the professional and scientific nature of the project, we also consult with researchers in related fields to adjust our research direction and research content.",{"type":12,"tag":83,"props":84,"children":86},"h3",{"id":85},"government-department",[87],{"type":18,"value":88},"Government Department",{"type":12,"tag":90,"props":91,"children":93},"h4",{"id":92},"bureau-of-ecology-environment-and-environmental-protection",[94],{"type":18,"value":95},"Bureau of Ecology Environment and Environmental Protection",{"type":12,"tag":21,"props":97,"children":98},{},[99],{"type":18,"value":100},"On May 23, 2024, our team members approached Ms. Luo, an official of Chongqing Ecological Environment and Environmental Protection Bureau, to consult the social needs of our project.\nFirst of all, we consulted the definition method, detection and registration process and control measures of endocrine disruptors. Luo pointed out that after confirming that a substance is polluting, the government departments will commission professional technical institutions to conduct research and investigation every year, and if the result is ultra-ultra-trace, relatively safe, no further measures will be taken. But if the concentration is high, the government will take measures and test the water quality of the test site or its source to track its possible source. In the analysis of new pollutants, including endocrine disruptors, government departments will conduct toxicological tests on chemical substances based on cutting-edge international research to determine that a substance may be harmful to the human body. This is a very important part of the testing process, because the government departments will concentrate the samples, which will lead to a variety of substances will be detected, and only when these substances are associated with toxicological effects and confirmed to be harmful to the human body, it is necessary to define endocrine disruptors and monitor. In terms of control, the government has corresponding emission standards for well-known endocrine disruptors; For new pollutants such as endocrine disruptors detected, the government will continue to confirm whether they belong to the emission substances already on the control list. For the situation where the pollutant index exceeds the standard, the government department will require the enterprise to take corresponding treatment measures and implement management measures such as regular review in the follow-up to supervise the rectification results.",{"type":12,"tag":21,"props":102,"children":103},{},[104],{"type":18,"value":105},"As for endocrine disruptors, Luo pointed out that in some studies, all organic compounds were classified as endocrine disruptors under investigation, which is not accurate; The current approach is to study each emerging substance independently and then classify it. The test site of endocrine disruptors is generally in other important water bodies such as drinking water sources, livestock and poultry farms and chemical plants and other emission sources.",{"type":12,"tag":21,"props":107,"children":108},{},[109],{"type":18,"value":110},"Secondly, we proposed a method to determine whether the water quality contains endocrine disruptors by using the biosensor constructed by the engineering bacteria that specifically recognizes endocrine disruptors. Luo first confirmed that in the environmental field, the emergency equipment launched abroad in the past few years does include microbial sensors and fluorescence detection technology to deal with sudden emergency environmental accidents. For endocrine disruptors, the government does not include them in routine testing tasks, but they are regularly included in testing efforts. The government prefers to use more accurate tests to detect endocrine disruptors. Therefore, from this point of view, improving the accuracy and sensitivity of the detection is also very beneficial and important.",{"type":12,"tag":21,"props":112,"children":113},{},[114],{"type":18,"value":115},"In short, Luo affirmed our project's detection method of using highly sensitive and specific biosensors for endocrine disruptors in the environment, which is very necessary and beneficial for government departments to detect endocrine disruptors, and our project is in line with the needs of government departments.",{"type":12,"tag":83,"props":117,"children":119},{"id":118},"environmental-testing-company",[120],{"type":18,"value":121},"Environmental Testing Company",{"type":12,"tag":90,"props":123,"children":125},{"id":124},"shanghai-environmental-monitoring-technology-co-ltd",[126],{"type":18,"value":127},"Shanghai Environmental Monitoring Technology Co., Ltd",{"type":12,"tag":21,"props":129,"children":130},{},[131],{"type":12,"tag":30,"props":132,"children":134},{"alt":5,"src":133},"https://static.igem.wiki/teams/5358/ihp/guo.png",[],{"type":12,"tag":21,"props":136,"children":137},{},[138],{"type":18,"value":139},"In order to continue to understand the definition method and testing registration process of new pollutants, on August 1, 2024, we found Mr. Guo, chief engineer of Shanghai Environmental Monitoring Technology Co., Ltd. for consultation.",{"type":12,"tag":21,"props":141,"children":142},{},[143],{"type":18,"value":144},"Guo pointed out that the determination of pollutants is first based on those already found by the country, and their emissions often exceed the prescribed standards, while classification and the formulation of standards are needed. The study of these pollutants needs to determine whether they have harmful effects on the human body through effect analysis, toxicology analysis and process analysis. Secondly, the use of foreign literature research combined with the actual situation in China to develop a standardized detection process to find endocrine disruptors. The definition of most new pollutants, especially endocrine disruptors, is that they are first found to be harmful to human health, and then they are analyzed in detail.",{"type":12,"tag":21,"props":146,"children":147},{},[148],{"type":18,"value":149},"For the analysis standards of new pollutants such as endocrine disruptors, Guo mentioned that with the development of industry, some new substances will be continuously studied, but whether it is harmful to the human body needs to be determined through a scientific judgment process, and if it is found to be harmful to the human body, it needs to be immediately included in the supervision. According to the requirements of the state, Shanghai has put the new pollutants to be studied into the supervision. Both testing organizations and industry systems are constantly developing methods to detect new pollutants, including endocrine disruptors, and current detection instruments are sophisticated and expensive.",{"type":12,"tag":21,"props":151,"children":152},{},[153],{"type":18,"value":154},"In the subsequent improvement process, the hardware device has undergone a simplified design to achieve the above design goals of portability and miniaturization.",{"type":12,"tag":21,"props":156,"children":157},{},[158],{"type":18,"value":159},"In the communication with Luo and Guo, they both affirmed our thinking and importance of detecting new pollutants such as endocrine pollutants through toxicological effects.",{"type":12,"tag":83,"props":161,"children":163},{"id":162},"factory-and-enterprise",[164],{"type":18,"value":165},"Factory and Enterprise",{"type":12,"tag":21,"props":167,"children":168},{},[169],{"type":18,"value":170},"Our next step is to understand the current status of the detection and analysis of endocrine disruptors from the perspective of relevant units and companies, as well as the specific needs currently existing, and ask whether there are applicable scenarios for the project. In accordance with Luo's previous guidance, we found a sewage plant and a water plant in Shanghai on August 1, 2024.",{"type":12,"tag":90,"props":172,"children":174},{"id":173},"shanghai-taihe-wastewater-treatment-plant",[175],{"type":18,"value":176},"Shanghai Taihe Wastewater Treatment Plant",{"type":12,"tag":21,"props":178,"children":179},{},[180],{"type":12,"tag":30,"props":181,"children":183},{"alt":5,"src":182},"https://static.igem.wiki/teams/5358/ihp/liu.png",[],{"type":12,"tag":21,"props":185,"children":186},{},[187],{"type":18,"value":188},"We have found Mr. Liu, director of Shanghai Taihe Sewage Plant. His reply to the above question is that the main pollutants under supervision of the sewage plant are Cod, Bod, ammonia, nitrogen and phosphorus. However, the pollutants under supervision of the sewage plant are to send water samples to testing institutions for testing, and domestic sewage involves few substances with toxicological properties.",{"type":12,"tag":21,"props":190,"children":191},{},[192],{"type":18,"value":193},"For our project, Mr. Liu said that due to the above reasons, the emergence of relevant technologies is temporarily lacking. Therefore, we should reflect on the use cases of our own projects and focus on providing services to government departments and environmental monitoring agencies.",{"type":12,"tag":90,"props":195,"children":197},{"id":196},"shanghai-nanhui-water-plant",[198],{"type":18,"value":199},"Shanghai Nanhui Water Plant",{"type":12,"tag":21,"props":201,"children":202},{},[203],{"type":12,"tag":30,"props":204,"children":206},{"alt":5,"src":205},"https://static.igem.wiki/teams/5358/ihp/ma.png",[],{"type":12,"tag":21,"props":208,"children":209},{},[210],{"type":18,"value":211},"In the drinking water industry, we found Mr. Ma, director of Shanghai Nanhui Water Plant, for consultation. In response to the above problems, the director pointed out that the detection of new pollutants in the drinking water industry, including endocrine disruptors, must use more expensive instruments, and the detection requirements and operations are more complicated; There is a need for emergency detection of source water for the use of biosensors. In view of our team's project, the factory director put forward that it can be applied in the rapid emergency monitoring of the above source water, and put forward the suggestions and technical requirements of our products for both qualitative and quantitative testing of drinking water source water.",{"type":12,"tag":21,"props":213,"children":214},{},[215],{"type":18,"value":216},"Therefore, for factories and enterprises, our project has a demand in the rapid emergency monitoring of drinking water source water, and we need to design a gate-based multi-toxicological effect screening sensor that can carry out qualitative and quantitative analysis on the basis of the project and is equipped with portable detection equipment.",{"type":12,"tag":21,"props":218,"children":219},{},[220,222],{"type":18,"value":221},"In short, the need for endocrine disruptor testing that we investigated is actually confirmed. At present, the detection of new pollutants including endocrine disruptors is more passive, and the method is more heavy on toxicological analysis, and has high cost and cumbersome operation. Therefore, there is a real need for a highly sensitive and specific detection of endocrine disruption and DNA damage in unknown samples, two important toxicological effects, and the hardware of such sensors must be portable and miniaturized.",{"type":12,"tag":30,"props":223,"children":225},{"alt":5,"src":224},"https://static.igem.wiki/teams/5358/ihp/projectpropose.png",[],{"type":12,"tag":83,"props":227,"children":229},{"id":228},"professional-research-personnel",[230],{"type":18,"value":231},"Professional research personnel",{"type":12,"tag":21,"props":233,"children":234},{},[235],{"type":12,"tag":30,"props":236,"children":238},{"alt":5,"src":237},"https://static.igem.wiki/teams/5358/ihp/yang.jpg",[],{"type":12,"tag":21,"props":240,"children":241},{},[242],{"type":18,"value":243},"We had an in-depth discussion on Modeling with Professor Yang Guanghui of China Agricultural University. He first gave a positive opinion of our idea. As the latest achievement in the field of bioinformatics, Alphafold3 has a high degree of confidence in the prediction of protein structure function, which can effectively guide our research in synthetic biology. But he also points out that the number and length of hydrogen bonds alone cannot accurately measure how folded a protein domain is. The main force in protein folding is hydrophobic interaction. However, there are no effective means to quantitatively measure the degree of protein folding and the strength of hydrophobic interaction. He encouraged us to work in this direction, hoping that we would be able to measure protein folding from other angles.",{"type":12,"tag":21,"props":245,"children":246},{},[247],{"type":18,"value":248},"Inspired by this, and after subsequent investigation, we will use Chimera1.13 software to analyze the hydrophobic interaction of the protein conformation obtained by molecular dynamics simulation (MDS), and measure the hydrophobic interaction intensity by comparing the hydrophobic potential around the recAN end and the C-end, so as to compare the degree of folding of this domain in the protein",{"type":12,"tag":13,"props":250,"children":252},{"id":251},"feedback-recollecting",[253],{"type":18,"value":254},"Feedback Recollecting",{"type":12,"tag":21,"props":256,"children":257},{},[258],{"type":18,"value":259},"We plan to bring the prototype design to endocrine disruptor experts and previous stakeholders, and continue to receive their comments and feedback to continue to adapt our project; At the same time, we will also find new innovation points and needs from the feedback, so as to launch a new round of \"BRIEF\" cycle in the future and complete the closed loop.",{"type":12,"tag":21,"props":261,"children":262},{},[263],{"type":12,"tag":30,"props":264,"children":266},{"alt":5,"src":265},"https://static.igem.wiki/teams/5358/ihp/executingandadjusting.png",[],{"title":5,"searchDepth":268,"depth":269,"links":270},1,3,[271,273,274,275,289],{"id":15,"depth":272,"text":19},2,{"id":41,"depth":272,"text":44},{"id":62,"depth":272,"text":65},{"id":73,"depth":272,"text":76,"children":276},[277,281,284,288],{"id":85,"depth":269,"text":88,"children":278},[279],{"id":92,"depth":280,"text":95},4,{"id":118,"depth":269,"text":121,"children":282},[283],{"id":124,"depth":280,"text":127},{"id":162,"depth":269,"text":165,"children":285},[286,287],{"id":173,"depth":280,"text":176},{"id":196,"depth":280,"text":199},{"id":228,"depth":269,"text":231},{"id":251,"depth":272,"text":254},"markdown","content:hp-ihp.md","content","hp-ihp.md","hp-ihp","md",1727871092464]
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.el-button--info:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button{--el-button-font-weight:var(--el-font-weight-primary);--el-button-border-color:var(--el-border-color);--el-button-bg-color:var(--el-fill-color-blank);--el-button-text-color:var(--el-text-color-regular);--el-button-disabled-text-color:var(--el-disabled-text-color);--el-button-disabled-bg-color:var(--el-fill-color-blank);--el-button-disabled-border-color:var(--el-border-color-light);--el-button-divide-border-color:hsla(0,0%,100%,.5);--el-button-hover-text-color:var(--el-color-primary);--el-button-hover-bg-color:var(--el-color-primary-light-9);--el-button-hover-border-color:var(--el-color-primary-light-7);--el-button-active-text-color:var(--el-button-hover-text-color);--el-button-active-border-color:var(--el-color-primary);--el-button-active-bg-color:var(--el-button-hover-bg-color);--el-button-outline-color:var(--el-color-primary-light-5);--el-button-hover-link-text-color:var(--el-color-info);--el-button-active-color:var(--el-text-color-primary);align-items:center;-webkit-appearance:none;background-color:var(--el-button-bg-color);border:var(--el-border);border-color:var(--el-button-border-color);box-sizing:border-box;color:var(--el-button-text-color);cursor:pointer;display:inline-flex;font-weight:var(--el-button-font-weight);height:32px;justify-content:center;line-height:1;outline:none;text-align:center;transition:.1s;-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-button:hover{background-color:var(--el-button-hover-bg-color);border-color:var(--el-button-hover-border-color);color:var(--el-button-hover-text-color);outline:none}.el-button:active{background-color:var(--el-button-active-bg-color);border-color:var(--el-button-active-border-color);color:var(--el-button-active-text-color);outline:none}.el-button:focus-visible{outline:2px 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1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper 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.el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags 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.el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large 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11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) 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cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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79.1666666667%;max-width:79.1666666667%}.el-col-xs-19.is-guttered{display:block}.el-col-xs-offset-19{margin-left:79.1666666667%}.el-col-xs-pull-19{position:relative;right:79.1666666667%}.el-col-xs-push-19{left:79.1666666667%;position:relative}.el-col-xs-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xs-20.is-guttered{display:block}.el-col-xs-offset-20{margin-left:83.3333333333%}.el-col-xs-pull-20{position:relative;right:83.3333333333%}.el-col-xs-push-20{left:83.3333333333%;position:relative}.el-col-xs-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xs-21.is-guttered{display:block}.el-col-xs-offset-21{margin-left:87.5%}.el-col-xs-pull-21{position:relative;right:87.5%}.el-col-xs-push-21{left:87.5%;position:relative}.el-col-xs-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 0%;max-width:0}.el-col-sm-0.is-guttered{display:none}.el-col-sm-offset-0{margin-left:0}.el-col-sm-pull-0{position:relative;right:0}.el-col-sm-push-0{left:0;position:relative}.el-col-sm-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-sm-1,.el-col-sm-1.is-guttered{display:block}.el-col-sm-offset-1{margin-left:4.1666666667%}.el-col-sm-pull-1{position:relative;right:4.1666666667%}.el-col-sm-push-1{left:4.1666666667%;position:relative}.el-col-sm-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-sm-2,.el-col-sm-2.is-guttered{display:block}.el-col-sm-offset-2{margin-left:8.3333333333%}.el-col-sm-pull-2{position:relative;right:8.3333333333%}.el-col-sm-push-2{left:8.3333333333%;position:relative}.el-col-sm-3{flex:0 0 12.5%;max-width:12.5%}.el-col-sm-3,.el-col-sm-3.is-guttered{display:block}.el-col-sm-offset-3{margin-left:12.5%}.el-col-sm-pull-3{position:relative;right:12.5%}.el-col-sm-push-3{left:12.5%;position:relative}.el-col-sm-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-sm-4,.el-col-sm-4.is-guttered{display:block}.el-col-sm-offset-4{margin-left:16.6666666667%}.el-col-sm-pull-4{position:relative;right:16.6666666667%}.el-col-sm-push-4{left:16.6666666667%;position:relative}.el-col-sm-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-sm-5,.el-col-sm-5.is-guttered{display:block}.el-col-sm-offset-5{margin-left:20.8333333333%}.el-col-sm-pull-5{position:relative;right:20.8333333333%}.el-col-sm-push-5{left:20.8333333333%;position:relative}.el-col-sm-6{flex:0 0 25%;max-width:25%}.el-col-sm-6,.el-col-sm-6.is-guttered{display:block}.el-col-sm-offset-6{margin-left:25%}.el-col-sm-pull-6{position:relative;right:25%}.el-col-sm-push-6{left:25%;position:relative}.el-col-sm-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 0%;max-width:0}.el-col-xl-0.is-guttered{display:none}.el-col-xl-offset-0{margin-left:0}.el-col-xl-pull-0{position:relative;right:0}.el-col-xl-push-0{left:0;position:relative}.el-col-xl-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-xl-1,.el-col-xl-1.is-guttered{display:block}.el-col-xl-offset-1{margin-left:4.1666666667%}.el-col-xl-pull-1{position:relative;right:4.1666666667%}.el-col-xl-push-1{left:4.1666666667%;position:relative}.el-col-xl-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-xl-2,.el-col-xl-2.is-guttered{display:block}.el-col-xl-offset-2{margin-left:8.3333333333%}.el-col-xl-pull-2{position:relative;right:8.3333333333%}.el-col-xl-push-2{left:8.3333333333%;position:relative}.el-col-xl-3{flex:0 0 12.5%;max-width:12.5%}.el-col-xl-3,.el-col-xl-3.is-guttered{display:block}.el-col-xl-offset-3{margin-left:12.5%}.el-col-xl-pull-3{position:relative;right:12.5%}.el-col-xl-push-3{left:12.5%;position:relative}.el-col-xl-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-xl-4,.el-col-xl-4.is-guttered{display:block}.el-col-xl-offset-4{margin-left:16.6666666667%}.el-col-xl-pull-4{position:relative;right:16.6666666667%}.el-col-xl-push-4{left:16.6666666667%;position:relative}.el-col-xl-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-xl-5,.el-col-xl-5.is-guttered{display:block}.el-col-xl-offset-5{margin-left:20.8333333333%}.el-col-xl-pull-5{position:relative;right:20.8333333333%}.el-col-xl-push-5{left:20.8333333333%;position:relative}.el-col-xl-6{flex:0 0 25%;max-width:25%}.el-col-xl-6,.el-col-xl-6.is-guttered{display:block}.el-col-xl-offset-6{margin-left:25%}.el-col-xl-pull-6{position:relative;right:25%}.el-col-xl-push-6{left:25%;position:relative}.el-col-xl-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-xl-10.is-guttered{display:block}.el-col-xl-offset-10{margin-left:41.6666666667%}.el-col-xl-pull-10{position:relative;right:41.6666666667%}.el-col-xl-push-10{left:41.6666666667%;position:relative}.el-col-xl-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-xl-11.is-guttered{display:block}.el-col-xl-offset-11{margin-left:45.8333333333%}.el-col-xl-pull-11{position:relative;right:45.8333333333%}.el-col-xl-push-11{left:45.8333333333%;position:relative}.el-col-xl-12{display:block;flex:0 0 50%;max-width:50%}.el-col-xl-12.is-guttered{display:block}.el-col-xl-offset-12{margin-left:50%}.el-col-xl-pull-12{position:relative;right:50%}.el-col-xl-push-12{left:50%;position:relative}.el-col-xl-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px;box-sizing:border-box;height:12px;position:relative;width:280px}.el-color-alpha-slider__bar{background:linear-gradient(to right,hsla(0,0%,100%,0) 0,var(--el-bg-color) 100%);height:100%;position:relative}.el-color-alpha-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-alpha-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-picker.is-focused .el-color-picker__trigger{border-color:var(--el-color-primary)}.el-color-picker.is-disabled .el-color-picker__trigger{cursor:not-allowed}.el-color-picker--large{height:40px}.el-color-picker--large .el-color-picker__trigger{height:40px;width:40px}.el-color-picker--large .el-color-picker__mask{height:38px;width:38px}.el-color-picker--small{height:24px}.el-color-picker--small .el-color-picker__trigger{height:24px;width:24px}.el-color-picker--small .el-color-picker__mask{height:22px;width:22px}.el-color-picker--small .el-color-picker__empty,.el-color-picker--small .el-color-picker__icon{transform:scale(.8)}.el-color-picker__mask{background-color:hsla(0,0%,100%,.7);border-radius:4px;cursor:not-allowed;height:30px;left:1px;position:absolute;top:1px;width:30px;z-index:1}.el-color-picker__trigger{align-items:center;border:1px solid var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker 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5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid 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var(--el-transition-duration)}.dialog-fade-leave-active .el-overlay-dialog{animation:dialog-fade-out var(--el-transition-duration)}@keyframes dialog-fade-in{0%{opacity:0;transform:translate3d(0,-20px,0)}to{opacity:1;transform:translateZ(0)}}@keyframes dialog-fade-out{0%{opacity:1;transform:translateZ(0)}to{opacity:0;transform:translate3d(0,-20px,0)}}@keyframes modal-fade-in{0%{opacity:0}to{opacity:1}}@keyframes modal-fade-out{0%{opacity:1}to{opacity:0}}.el-divider{position:relative}.el-divider--horizontal{border-top:1px var(--el-border-color) var(--el-border-style);display:block;height:1px;margin:24px 0;width:100%}.el-divider--vertical{border-left:1px var(--el-border-color) var(--el-border-style);display:inline-block;height:1em;margin:0 8px;position:relative;vertical-align:middle;width:1px}.el-divider__text{background-color:var(--el-bg-color);color:var(--el-text-color-primary);font-size:14px;font-weight:500;padding:0 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.ttb{transform:translate(0)}.el-drawer__sr-focus:focus{outline:none!important}.el-drawer__header{align-items:center;color:#72767b;display:flex;margin-bottom:32px;padding:var(--el-drawer-padding-primary);padding-bottom:0}.el-drawer__header>:first-child{flex:1}.el-drawer__title{flex:1;font-size:16px;line-height:inherit;margin:0}.el-drawer__footer{padding:var(--el-drawer-padding-primary);padding-top:10px;text-align:right}.el-drawer__close-btn{background-color:transparent;border:none;color:inherit;cursor:pointer;display:inline-flex;font-size:var(--el-font-size-extra-large);outline:none}.el-drawer__close-btn:focus i,.el-drawer__close-btn:hover 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.el-popper__arrow:before{border:1px solid var(--el-border-color-light)}.el-dropdown__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-dropdown__popper .el-dropdown-menu{border:none}.el-dropdown__popper .el-dropdown__popper-selfdefine{outline:none}.el-dropdown__popper .el-scrollbar__bar{z-index:calc(var(--el-dropdown-menu-index) + 1)}.el-dropdown__popper .el-dropdown__list{box-sizing:border-box;list-style:none;margin:0;padding:0}.el-dropdown 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var(--el-transition-duration);width:var(--el-notification-width);z-index:9999}.el-notification.right{right:16px}.el-notification.left{left:16px}.el-notification__group{margin-left:var(--el-notification-group-margin-left);margin-right:var(--el-notification-group-margin-right)}.el-notification__title{color:var(--el-notification-title-color);font-size:var(--el-notification-title-font-size);font-weight:700;line-height:var(--el-notification-icon-size);margin:0}.el-notification__content{color:var(--el-notification-content-color);font-size:var(--el-notification-content-font-size);line-height:24px;margin:6px 0 0}.el-notification__content p{margin:0}.el-notification .el-notification__icon{font-size:var(--el-notification-icon-size);height:var(--el-notification-icon-size);width:var(--el-notification-icon-size)}.el-notification .el-notification__closeBtn{color:var(--el-notification-close-color);cursor:pointer;font-size:var(--el-notification-close-font-size);position:absolute;right:15px;top:18px}.el-notification .el-notification__closeBtn:hover{color:var(--el-notification-close-hover-color)}.el-notification .el-notification--success{--el-notification-icon-color:var(--el-color-success);color:var(--el-notification-icon-color)}.el-notification .el-notification--info{--el-notification-icon-color:var(--el-color-info);color:var(--el-notification-icon-color)}.el-notification .el-notification--warning{--el-notification-icon-color:var(--el-color-warning);color:var(--el-notification-icon-color)}.el-notification .el-notification--error{--el-notification-icon-color:var(--el-color-error);color:var(--el-notification-icon-color)}.el-notification-fade-enter-from.right{right:0;transform:translateX(100%)}.el-notification-fade-enter-from.left{left:0;transform:translateX(-100%)}.el-notification-fade-leave-to{opacity:0}.el-overlay{background-color:var(--el-overlay-color-lighter);bottom:0;height:100%;left:0;overflow:auto;position:fixed;right:0;top:0;z-index:2000}.el-overlay .el-overlay-root{height:0}.el-page-header.is-contentful .el-page-header__main{border-top:1px solid var(--el-border-color-light);margin-top:16px}.el-page-header__header{align-items:center;display:flex;justify-content:space-between;line-height:24px}.el-page-header__left{align-items:center;display:flex;margin-right:40px;position:relative}.el-page-header__back{align-items:center;cursor:pointer;display:flex}.el-page-header__left .el-divider--vertical{margin:0 16px}.el-page-header__icon{align-items:center;display:flex;font-size:16px;margin-right:10px}.el-page-header__icon .el-icon{font-size:inherit}.el-page-header__title{font-size:14px;font-weight:500}.el-page-header__content{color:var(--el-text-color-primary);font-size:18px}.el-page-header__breadcrumb{margin-bottom:16px}.el-pagination{--el-pagination-font-size:14px;--el-pagination-bg-color:var(--el-fill-color-blank);--el-pagination-text-color:var(--el-text-color-primary);--el-pagination-border-radius:2px;--el-pagination-button-color:var(--el-text-color-primary);--el-pagination-button-width:32px;--el-pagination-button-height:32px;--el-pagination-button-disabled-color:var(--el-text-color-placeholder);--el-pagination-button-disabled-bg-color:var(--el-fill-color-blank);--el-pagination-button-bg-color:var(--el-fill-color);--el-pagination-hover-color:var(--el-color-primary);--el-pagination-font-size-small:12px;--el-pagination-button-width-small:24px;--el-pagination-button-height-small:24px;--el-pagination-button-width-large:40px;--el-pagination-button-height-large:40px;--el-pagination-item-gap:16px;align-items:center;color:var(--el-pagination-text-color);display:flex;font-size:var(--el-pagination-font-size);font-weight:400;white-space:nowrap}.el-pagination .el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small 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.el-transfer-panel__header{align-items:center;background:var(--el-transfer-panel-header-bg-color);border:1px solid var(--el-transfer-border-color);border-top-left-radius:var(--el-transfer-border-radius);border-top-right-radius:var(--el-transfer-border-radius);box-sizing:border-box;color:var(--el-color-black);display:flex;height:var(--el-transfer-panel-header-height);margin:0;padding-left:15px}.el-transfer-panel .el-transfer-panel__header .el-checkbox{align-items:center;display:flex;position:relative;width:100%}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label{color:var(--el-text-color-primary);font-size:16px;font-weight:400}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label span{color:var(--el-text-color-secondary);font-size:12px;font-weight:400;position:absolute;right:15px;top:50%;transform:translate3d(0,-50%,0)}.el-transfer-panel .el-transfer-panel__footer{background:var(--el-bg-color-overlay);border:1px solid 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0;overflow:hidden;padding:0;width:var(--el-upload-list-picture-card-size)}.el-upload-list--picture-card .el-upload-list__item .el-icon--check,.el-upload-list--picture-card .el-upload-list__item .el-icon--circle-check{color:#fff}.el-upload-list--picture-card .el-upload-list__item .el-icon--close{display:none}.el-upload-list--picture-card .el-upload-list__item:hover .el-upload-list__item-status-label{display:block;opacity:0}.el-upload-list--picture-card .el-upload-list__item:hover .el-progress__text{display:block}.el-upload-list--picture-card .el-upload-list__item .el-upload-list__item-name{display:none}.el-upload-list--picture-card .el-upload-list__item-thumbnail{height:100%;-o-object-fit:contain;object-fit:contain;width:100%}.el-upload-list--picture-card .el-upload-list__item-status-label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-list--picture-card .el-upload-list__item-status-label 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img{display:block;height:100%;width:100%}.el-upload-cover__label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-cover__label i{color:#fff;font-size:12px;margin-top:11px;transform:rotate(-45deg)}.el-upload-cover__progress{display:inline-block;position:static;vertical-align:middle;width:243px}.el-upload-cover__progress+.el-upload__inner{opacity:0}.el-upload-cover__content{height:100%;left:0;position:absolute;top:0;width:100%}.el-upload-cover__interact{background-color:var(--el-overlay-color-light);bottom:0;height:100%;left:0;position:absolute;text-align:center;width:100%}.el-upload-cover__interact .btn{color:#fff;cursor:pointer;display:inline-block;font-size:14px;margin-top:60px;transition:var(--el-transition-md-fade);vertical-align:middle}.el-upload-cover__interact .btn i{margin-top:0}.el-upload-cover__interact .btn span{opacity:0;transition:opacity .15s linear}.el-upload-cover__interact 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var(--el-border-color-light)}.el-popper.is-light>.el-popper__arrow:before{right:0}.el-popper.is-pure{padding:0}.el-popper__arrow,.el-popper__arrow:before{height:10px;position:absolute;width:10px;z-index:-1}.el-popper__arrow:before{background:var(--el-text-color-primary);box-sizing:border-box;content:" 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tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><div class="el-menu--vertical el-menu--popup-container"><ul class="el-menu el-menu--popup el-menu--popup-right-start" style="--el-menu-level:1;"><!--[--><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Members<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Attributions<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Contribution<!--]--><!--[--><!--]--><!--]--></li><!--]--></ul></div><!--]--><!--v-if--><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2011;position:absolute;left:0;top:0;display:none;" class="el-popper is-pure is-light" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><div class="el-menu--vertical el-menu--popup-container"><ul class="el-menu el-menu--popup el-menu--popup-right-start" 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start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Wet Lab<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Dry Lab<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 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role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Human Practices<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li></ul><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-mobile px-10 flex lg:hidden"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 2x" class="cursor-pointer py-2" src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div class="grow"></div><!--[--><span class="el-avatar el-avatar--icon el-avatar--circle self-center cursor-pointer el-tooltip__trigger el-tooltip__trigger" style=""><i class="el-icon" style=""><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M160 448a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zM160 896a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32z"></path></svg><!--]--></i></span><!--teleport start--><!--teleport end--><!--]--></ul></header><div class="pt-[8rem]"><!--[--><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="cover" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/hp.webp 1x, https://static.igem.wiki/teams/5358/banner/hp.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/hp.webp" data-v-0156a899><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg" data-v-0156a899>Integrated HP</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10" data-v-0156a899><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[400px] md:mr-10" data-v-0156a899><h1 class="" data-v-0156a899>TOC</h1><ul data-v-0156a899><!--[--><li class="my-5" data-v-0156a899><a class="cursor-pointer" data-v-0156a899>Overview</a><ul class="hidden pl-5 pt-2" data-v-0156a899><!--[--><!--]--></ul></li><li class="my-5" data-v-0156a899><a class="cursor-pointer" data-v-0156a899>Brainstorming</a><ul class="hidden pl-5 pt-2" data-v-0156a899><!--[--><!--]--></ul></li><li class="my-5" data-v-0156a899><a class="cursor-pointer" data-v-0156a899>Researching</a><ul class="hidden pl-5 pt-2" data-v-0156a899><!--[--><!--]--></ul></li><li class="my-5" data-v-0156a899><a class="cursor-pointer" data-v-0156a899>Insight Integrating</a><ul class="hidden pl-5 pt-2" data-v-0156a899><!--[--><!--[--><li class="text-[#fff] font-normal mb-2 text-sm xl:text-base" data-v-0156a899><a class="cursor-pointer" data-v-0156a899>Government Department</a></li><!--]--><!--[--><li class="text-[#fff] font-normal mb-2 text-sm xl:text-base" data-v-0156a899><a class="cursor-pointer" data-v-0156a899>Environmental Testing Company</a></li><!--]--><!--[--><li class="text-[#fff] font-normal mb-2 text-sm xl:text-base" data-v-0156a899><a class="cursor-pointer" data-v-0156a899>Factory and Enterprise</a></li><!--]--><!--[--><li class="text-[#fff] font-normal mb-2 text-sm xl:text-base" data-v-0156a899><a class="cursor-pointer" data-v-0156a899>Professional research personnel</a></li><!--]--><!--]--></ul></li><li class="my-5" data-v-0156a899><a class="cursor-pointer" data-v-0156a899>Feedback Recollecting</a><ul class="hidden pl-5 pt-2" data-v-0156a899><!--[--><!--]--></ul></li><!--]--></ul></aside><div class="content" data-v-0156a899><h2 id="overview"><!--[-->Overview<!--]--></h2><p><!--[-->The core of HP is to present project designs and products to the community and stakeholders, get feedback, integrate feedback into the project, and constantly adjust and modify the project according to the guidance of the feedback. This year, we proposed the &quot;BRIEF&quot; periodic iteration method to complete IHP. The aim is to ensure that projects meet practical needs, continuously improve project performance through feedback, meet the needs of stakeholders, and contribute to society in order to build projects that are good and responsible for the world.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/ihp/brief.jpg 1x, https://static.igem.wiki/teams/5358/ihp/brief.jpg 2x" src="https://static.igem.wiki/teams/5358/ihp/brief.jpg"><!--]--></p><p><!--[-->The &quot;BRIEF&quot; concept consists of five phases of IHP implementation, They are Brainstorming, Researching, Insight Integrating, Executing and Adjusting, and Feedback Recollecting five aspects. Among them, Executing and Adjusting the project is a bridge connecting Brainstorming, Researching, Insight Integrating and Feedback Recollecting. Throughout IHP, we build a cycle of finding meaning, reaching out, and connecting our projects to the world.<!--]--></p><h2 id="brainstorming"><!--[-->Brainstorming<!--]--></h2><p><!--[-->Our team is focusing on detecting new pollutants this year. At the beginning of the project, we carried out extensive research on new pollutants, and focused on endocrine disruptors, microplastics, antibiotics, persistent pollutants four, because of their greater harm and detection methods are expensive and difficult to operate.<!--]--></p><p><!--[-->Since then, endocrine disruptors have been screened according to the difficulty of detection, the harm to the environment and the significance of positive results. In the traditional evaluation system of endocrine disruptors, most of the main methods are to detect their binding with hormone receptors. However, due to the extensive and complex toxic effects of endocrine disruptors and their metabolites on organisms, the traditional detection methods are obviously inadequate.<!--]--></p><p><!--[-->The result of our brainstorming is to build a gate-based multi-toxicological screening sensor that can simultaneously detect endocrine disrupting effects and DNA damage effects in factory and enterprise products, or unknown samples in the environment.<!--]--></p><h2 id="researching"><!--[-->Researching<!--]--></h2><p><!--[-->After determining the study of endocrine disruptors, we screened out methods that could detect endocrine disruptors through literature research and conducted feasibility analysis. Finally, a method was established to detect endocrine disruptors by constructing hormone analogues sensitive peptides and coupled with Escherichia coli SOS oxidative damage response system and hrp amplifier for detecting DNA damage effects to construct a gated multi-toxicological effects screening sensor design. In addition, we investigated the contained peptide sequence and found that the number of bases was too large, which would cause a heavy burden on Escherichia coli. Therefore, the components for detecting the two effects were divided into two bacteria, and the LuxI/LuxR system was used for communication. Biosensors use fluorescent protein reports.
 This is the initial design of our project.<!--]--></p><h2 id="insight-integrating"><!--[-->Insight Integrating<!--]--></h2><p><!--[-->In order to identify the social needs of our project with stakeholders, we contacted environmental testing companies, government departments, factories and wastewater treatment plants to ask about the needs and status of pollutant monitoring. In addition, in order to enhance the professional and scientific nature of the project, we also consult with researchers in related fields to adjust our research direction and research content.<!--]--></p><h3 id="government-department"><!--[-->Government Department<!--]--></h3><h4 id="bureau-of-ecology-environment-and-environmental-protection"><!--[-->Bureau of Ecology Environment and Environmental Protection<!--]--></h4><p><!--[-->On May 23, 2024, our team members approached Ms. Luo, an official of Chongqing Ecological Environment and Environmental Protection Bureau, to consult the social needs of our project.
-First of all, we consulted the definition method, detection and registration process and control measures of endocrine disruptors. Luo pointed out that after confirming that a substance is polluting, the government departments will commission professional technical institutions to conduct research and investigation every year, and if the result is ultra-ultra-trace, relatively safe, no further measures will be taken. But if the concentration is high, the government will take measures and test the water quality of the test site or its source to track its possible source. In the analysis of new pollutants, including endocrine disruptors, government departments will conduct toxicological tests on chemical substances based on cutting-edge international research to determine that a substance may be harmful to the human body. This is a very important part of the testing process, because the government departments will concentrate the samples, which will lead to a variety of substances will be detected, and only when these substances are associated with toxicological effects and confirmed to be harmful to the human body, it is necessary to define endocrine disruptors and monitor. In terms of control, the government has corresponding emission standards for well-known endocrine disruptors; For new pollutants such as endocrine disruptors detected, the government will continue to confirm whether they belong to the emission substances already on the control list. For the situation where the pollutant index exceeds the standard, the government department will require the enterprise to take corresponding treatment measures and implement management measures such as regular review in the follow-up to supervise the rectification results.<!--]--></p><p><!--[-->As for endocrine disruptors, Luo pointed out that in some studies, all organic compounds were classified as endocrine disruptors under investigation, which is not accurate; The current approach is to study each emerging substance independently and then classify it. The test site of endocrine disruptors is generally in other important water bodies such as drinking water sources, livestock and poultry farms and chemical plants and other emission sources.<!--]--></p><p><!--[-->Secondly, we proposed a method to determine whether the water quality contains endocrine disruptors by using the biosensor constructed by the engineering bacteria that specifically recognizes endocrine disruptors. Luo first confirmed that in the environmental field, the emergency equipment launched abroad in the past few years does include microbial sensors and fluorescence detection technology to deal with sudden emergency environmental accidents. For endocrine disruptors, the government does not include them in routine testing tasks, but they are regularly included in testing efforts. The government prefers to use more accurate tests to detect endocrine disruptors. Therefore, from this point of view, improving the accuracy and sensitivity of the detection is also very beneficial and important.<!--]--></p><p><!--[-->In short, Luo affirmed our project&#39;s detection method of using highly sensitive and specific biosensors for endocrine disruptors in the environment, which is very necessary and beneficial for government departments to detect endocrine disruptors, and our project is in line with the needs of government departments.<!--]--></p><h3 id="environmental-testing-company"><!--[-->Environmental Testing Company<!--]--></h3><h4 id="shanghai-environmental-monitoring-technology-co-ltd"><!--[-->Shanghai Environmental Monitoring Technology Co., Ltd<!--]--></h4><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/ihp/guo.png 1x, https://static.igem.wiki/teams/5358/ihp/guo.png 2x" src="https://static.igem.wiki/teams/5358/ihp/guo.png"><!--]--></p><p><!--[-->In order to continue to understand the definition method and testing registration process of new pollutants, on August 1, 2024, we found Mr. Guo, chief engineer of Shanghai Environmental Monitoring Technology Co., Ltd. for consultation.<!--]--></p><p><!--[-->Guo pointed out that the determination of pollutants is first based on those already found by the country, and their emissions often exceed the prescribed standards, while classification and the formulation of standards are needed. The study of these pollutants needs to determine whether they have harmful effects on the human body through effect analysis, toxicology analysis and process analysis. Secondly, the use of foreign literature research combined with the actual situation in China to develop a standardized detection process to find endocrine disruptors. The definition of most new pollutants, especially endocrine disruptors, is that they are first found to be harmful to human health, and then they are analyzed in detail.<!--]--></p><p><!--[-->For the analysis standards of new pollutants such as endocrine disruptors, Guo mentioned that with the development of industry, some new substances will be continuously studied, but whether it is harmful to the human body needs to be determined through a scientific judgment process, and if it is found to be harmful to the human body, it needs to be immediately included in the supervision. According to the requirements of the state, Shanghai has put the new pollutants to be studied into the supervision. Both testing organizations and industry systems are constantly developing methods to detect new pollutants, including endocrine disruptors, and current detection instruments are sophisticated and expensive.<!--]--></p><p><!--[-->In the subsequent improvement process, the hardware device has undergone a simplified design to achieve the above design goals of portability and miniaturization.<!--]--></p><p><!--[-->In the communication with Luo and Guo, they both affirmed our thinking and importance of detecting new pollutants such as endocrine pollutants through toxicological effects.<!--]--></p><h3 id="factory-and-enterprise"><!--[-->Factory and Enterprise<!--]--></h3><p><!--[-->Our next step is to understand the current status of the detection and analysis of endocrine disruptors from the perspective of relevant units and companies, as well as the specific needs currently existing, and ask whether there are applicable scenarios for the project. In accordance with Luo&#39;s previous guidance, we found a sewage plant and a water plant in Shanghai on August 1, 2024.<!--]--></p><h4 id="shanghai-taihe-wastewater-treatment-plant"><!--[-->Shanghai Taihe Wastewater Treatment Plant<!--]--></h4><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/ihp/liu.png 1x, https://static.igem.wiki/teams/5358/ihp/liu.png 2x" src="https://static.igem.wiki/teams/5358/ihp/liu.png"><!--]--></p><p><!--[-->We have found Mr. Liu, director of Shanghai Taihe Sewage Plant. His reply to the above question is that the main pollutants under supervision of the sewage plant are Cod, Bod, ammonia, nitrogen and phosphorus. However, the pollutants under supervision of the sewage plant are to send water samples to testing institutions for testing, and domestic sewage involves few substances with toxicological properties.<!--]--></p><p><!--[-->For our project, Mr. Liu said that due to the above reasons, the emergence of relevant technologies is temporarily lacking. Therefore, we should reflect on the use cases of our own projects and focus on providing services to government departments and environmental monitoring agencies.<!--]--></p><h4 id="shanghai-nanhui-water-plant"><!--[-->Shanghai Nanhui Water Plant<!--]--></h4><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/ihp/ma.png 1x, https://static.igem.wiki/teams/5358/ihp/ma.png 2x" src="https://static.igem.wiki/teams/5358/ihp/ma.png"><!--]--></p><p><!--[-->In the drinking water industry, we found Mr. Ma, director of Shanghai Nanhui Water Plant, for consultation. In response to the above problems, the director pointed out that the detection of new pollutants in the drinking water industry, including endocrine disruptors, must use more expensive instruments, and the detection requirements and operations are more complicated; There is a need for emergency detection of source water for the use of biosensors. In view of our team&#39;s project, the factory director put forward that it can be applied in the rapid emergency monitoring of the above source water, and put forward the suggestions and technical requirements of our products for both qualitative and quantitative testing of drinking water source water.<!--]--></p><p><!--[-->Therefore, for factories and enterprises, our project has a demand in the rapid emergency monitoring of drinking water source water, and we need to design a gate-based multi-toxicological effect screening sensor that can carry out qualitative and quantitative analysis on the basis of the project and is equipped with portable detection equipment.<!--]--></p><p><!--[-->In short, the need for endocrine disruptor testing that we investigated is actually confirmed. At present, the detection of new pollutants including endocrine disruptors is more passive, and the method is more heavy on toxicological analysis, and has high cost and cumbersome operation. Therefore, there is a real need for a highly sensitive and specific detection of endocrine disruption and DNA damage in unknown samples, two important toxicological effects, and the hardware of such sensors must be portable and miniaturized.<img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/ihp/projectpropose.png 1x, https://static.igem.wiki/teams/5358/ihp/projectpropose.png 2x" src="https://static.igem.wiki/teams/5358/ihp/projectpropose.png"><!--]--></p><h3 id="professional-research-personnel"><!--[-->Professional research personnel<!--]--></h3><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/ihp/yang.jpg 1x, https://static.igem.wiki/teams/5358/ihp/yang.jpg 2x" src="https://static.igem.wiki/teams/5358/ihp/yang.jpg"><!--]--></p><p><!--[-->We had an in-depth discussion on Modeling with Professor Yang Guanghui of China Agricultural University. He first gave a positive opinion of our idea. As the latest achievement in the field of bioinformatics, Alphafold3 has a high degree of confidence in the prediction of protein structure function, which can effectively guide our research in synthetic biology. But he also points out that the number and length of hydrogen bonds alone cannot accurately measure how folded a protein domain is. The main force in protein folding is hydrophobic interaction. However, there are no effective means to quantitatively measure the degree of protein folding and the strength of hydrophobic interaction. He encouraged us to work in this direction, hoping that we would be able to measure protein folding from other angles.<!--]--></p><p><!--[-->Inspired by this, and after subsequent investigation, we will use Chimera1.13 software to analyze the hydrophobic interaction of the protein conformation obtained by molecular dynamics simulation (MDS), and measure the hydrophobic interaction intensity by comparing the hydrophobic potential around the recAN end and the C-end, so as to compare the degree of folding of this domain in the protein<!--]--></p><h2 id="feedback-recollecting"><!--[-->Feedback Recollecting<!--]--></h2><p><!--[-->We plan to bring the prototype design to endocrine disruptor experts and previous stakeholders, and continue to receive their comments and feedback to continue to adapt our project; At the same time, we will also find new innovation points and needs from the feedback, so as to launch a new round of &quot;BRIEF&quot; cycle in the future and complete the closed loop.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/ihp/executingandadjusting.png 1x, https://static.igem.wiki/teams/5358/ihp/executingandadjusting.png 2x" src="https://static.igem.wiki/teams/5358/ihp/executingandadjusting.png"><!--]--></p></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a aria-current="page" href="/bit/human-practices" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/human-practices/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-dSb1ehdCUe":11},null,"/human-practices",1727868995423]</script>
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+First of all, we consulted the definition method, detection and registration process and control measures of endocrine disruptors. Luo pointed out that after confirming that a substance is polluting, the government departments will commission professional technical institutions to conduct research and investigation every year, and if the result is ultra-ultra-trace, relatively safe, no further measures will be taken. But if the concentration is high, the government will take measures and test the water quality of the test site or its source to track its possible source. In the analysis of new pollutants, including endocrine disruptors, government departments will conduct toxicological tests on chemical substances based on cutting-edge international research to determine that a substance may be harmful to the human body. This is a very important part of the testing process, because the government departments will concentrate the samples, which will lead to a variety of substances will be detected, and only when these substances are associated with toxicological effects and confirmed to be harmful to the human body, it is necessary to define endocrine disruptors and monitor. In terms of control, the government has corresponding emission standards for well-known endocrine disruptors; For new pollutants such as endocrine disruptors detected, the government will continue to confirm whether they belong to the emission substances already on the control list. For the situation where the pollutant index exceeds the standard, the government department will require the enterprise to take corresponding treatment measures and implement management measures such as regular review in the follow-up to supervise the rectification results.<!--]--></p><p><!--[-->As for endocrine disruptors, Luo pointed out that in some studies, all organic compounds were classified as endocrine disruptors under investigation, which is not accurate; The current approach is to study each emerging substance independently and then classify it. The test site of endocrine disruptors is generally in other important water bodies such as drinking water sources, livestock and poultry farms and chemical plants and other emission sources.<!--]--></p><p><!--[-->Secondly, we proposed a method to determine whether the water quality contains endocrine disruptors by using the biosensor constructed by the engineering bacteria that specifically recognizes endocrine disruptors. Luo first confirmed that in the environmental field, the emergency equipment launched abroad in the past few years does include microbial sensors and fluorescence detection technology to deal with sudden emergency environmental accidents. For endocrine disruptors, the government does not include them in routine testing tasks, but they are regularly included in testing efforts. The government prefers to use more accurate tests to detect endocrine disruptors. Therefore, from this point of view, improving the accuracy and sensitivity of the detection is also very beneficial and important.<!--]--></p><p><!--[-->In short, Luo affirmed our project&#39;s detection method of using highly sensitive and specific biosensors for endocrine disruptors in the environment, which is very necessary and beneficial for government departments to detect endocrine disruptors, and our project is in line with the needs of government departments.<!--]--></p><h3 id="environmental-testing-company"><!--[-->Environmental Testing Company<!--]--></h3><h4 id="shanghai-environmental-monitoring-technology-co-ltd"><!--[-->Shanghai Environmental Monitoring Technology Co., Ltd<!--]--></h4><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/ihp/guo.png 1x, https://static.igem.wiki/teams/5358/ihp/guo.png 2x" src="https://static.igem.wiki/teams/5358/ihp/guo.png"><!--]--></p><p><!--[-->In order to continue to understand the definition method and testing registration process of new pollutants, on August 1, 2024, we found Mr. Guo, chief engineer of Shanghai Environmental Monitoring Technology Co., Ltd. for consultation.<!--]--></p><p><!--[-->Guo pointed out that the determination of pollutants is first based on those already found by the country, and their emissions often exceed the prescribed standards, while classification and the formulation of standards are needed. The study of these pollutants needs to determine whether they have harmful effects on the human body through effect analysis, toxicology analysis and process analysis. Secondly, the use of foreign literature research combined with the actual situation in China to develop a standardized detection process to find endocrine disruptors. The definition of most new pollutants, especially endocrine disruptors, is that they are first found to be harmful to human health, and then they are analyzed in detail.<!--]--></p><p><!--[-->For the analysis standards of new pollutants such as endocrine disruptors, Guo mentioned that with the development of industry, some new substances will be continuously studied, but whether it is harmful to the human body needs to be determined through a scientific judgment process, and if it is found to be harmful to the human body, it needs to be immediately included in the supervision. According to the requirements of the state, Shanghai has put the new pollutants to be studied into the supervision. Both testing organizations and industry systems are constantly developing methods to detect new pollutants, including endocrine disruptors, and current detection instruments are sophisticated and expensive.<!--]--></p><p><!--[-->In the subsequent improvement process, the hardware device has undergone a simplified design to achieve the above design goals of portability and miniaturization.<!--]--></p><p><!--[-->In the communication with Luo and Guo, they both affirmed our thinking and importance of detecting new pollutants such as endocrine pollutants through toxicological effects.<!--]--></p><h3 id="factory-and-enterprise"><!--[-->Factory and Enterprise<!--]--></h3><p><!--[-->Our next step is to understand the current status of the detection and analysis of endocrine disruptors from the perspective of relevant units and companies, as well as the specific needs currently existing, and ask whether there are applicable scenarios for the project. In accordance with Luo&#39;s previous guidance, we found a sewage plant and a water plant in Shanghai on August 1, 2024.<!--]--></p><h4 id="shanghai-taihe-wastewater-treatment-plant"><!--[-->Shanghai Taihe Wastewater Treatment Plant<!--]--></h4><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/ihp/liu.png 1x, https://static.igem.wiki/teams/5358/ihp/liu.png 2x" src="https://static.igem.wiki/teams/5358/ihp/liu.png"><!--]--></p><p><!--[-->We have found Mr. Liu, director of Shanghai Taihe Sewage Plant. His reply to the above question is that the main pollutants under supervision of the sewage plant are Cod, Bod, ammonia, nitrogen and phosphorus. However, the pollutants under supervision of the sewage plant are to send water samples to testing institutions for testing, and domestic sewage involves few substances with toxicological properties.<!--]--></p><p><!--[-->For our project, Mr. Liu said that due to the above reasons, the emergence of relevant technologies is temporarily lacking. Therefore, we should reflect on the use cases of our own projects and focus on providing services to government departments and environmental monitoring agencies.<!--]--></p><h4 id="shanghai-nanhui-water-plant"><!--[-->Shanghai Nanhui Water Plant<!--]--></h4><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/ihp/ma.png 1x, https://static.igem.wiki/teams/5358/ihp/ma.png 2x" src="https://static.igem.wiki/teams/5358/ihp/ma.png"><!--]--></p><p><!--[-->In the drinking water industry, we found Mr. Ma, director of Shanghai Nanhui Water Plant, for consultation. In response to the above problems, the director pointed out that the detection of new pollutants in the drinking water industry, including endocrine disruptors, must use more expensive instruments, and the detection requirements and operations are more complicated; There is a need for emergency detection of source water for the use of biosensors. In view of our team&#39;s project, the factory director put forward that it can be applied in the rapid emergency monitoring of the above source water, and put forward the suggestions and technical requirements of our products for both qualitative and quantitative testing of drinking water source water.<!--]--></p><p><!--[-->Therefore, for factories and enterprises, our project has a demand in the rapid emergency monitoring of drinking water source water, and we need to design a gate-based multi-toxicological effect screening sensor that can carry out qualitative and quantitative analysis on the basis of the project and is equipped with portable detection equipment.<!--]--></p><p><!--[-->In short, the need for endocrine disruptor testing that we investigated is actually confirmed. At present, the detection of new pollutants including endocrine disruptors is more passive, and the method is more heavy on toxicological analysis, and has high cost and cumbersome operation. Therefore, there is a real need for a highly sensitive and specific detection of endocrine disruption and DNA damage in unknown samples, two important toxicological effects, and the hardware of such sensors must be portable and miniaturized.<img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/ihp/projectpropose.png 1x, https://static.igem.wiki/teams/5358/ihp/projectpropose.png 2x" src="https://static.igem.wiki/teams/5358/ihp/projectpropose.png"><!--]--></p><h3 id="professional-research-personnel"><!--[-->Professional research personnel<!--]--></h3><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/ihp/yang.jpg 1x, https://static.igem.wiki/teams/5358/ihp/yang.jpg 2x" src="https://static.igem.wiki/teams/5358/ihp/yang.jpg"><!--]--></p><p><!--[-->We had an in-depth discussion on Modeling with Professor Yang Guanghui of China Agricultural University. He first gave a positive opinion of our idea. As the latest achievement in the field of bioinformatics, Alphafold3 has a high degree of confidence in the prediction of protein structure function, which can effectively guide our research in synthetic biology. But he also points out that the number and length of hydrogen bonds alone cannot accurately measure how folded a protein domain is. The main force in protein folding is hydrophobic interaction. However, there are no effective means to quantitatively measure the degree of protein folding and the strength of hydrophobic interaction. He encouraged us to work in this direction, hoping that we would be able to measure protein folding from other angles.<!--]--></p><p><!--[-->Inspired by this, and after subsequent investigation, we will use Chimera1.13 software to analyze the hydrophobic interaction of the protein conformation obtained by molecular dynamics simulation (MDS), and measure the hydrophobic interaction intensity by comparing the hydrophobic potential around the recAN end and the C-end, so as to compare the degree of folding of this domain in the protein<!--]--></p><h2 id="feedback-recollecting"><!--[-->Feedback Recollecting<!--]--></h2><p><!--[-->We plan to bring the prototype design to endocrine disruptor experts and previous stakeholders, and continue to receive their comments and feedback to continue to adapt our project; At the same time, we will also find new innovation points and needs from the feedback, so as to launch a new round of &quot;BRIEF&quot; cycle in the future and complete the closed loop.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt data-nuxt-img srcset="https://static.igem.wiki/teams/5358/ihp/executingandadjusting.png 1x, https://static.igem.wiki/teams/5358/ihp/executingandadjusting.png 2x" src="https://static.igem.wiki/teams/5358/ihp/executingandadjusting.png"><!--]--></p></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a aria-current="page" href="/bit/human-practices" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/human-practices/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-dSb1ehdCUe":11},null,"/human-practices",1727871092464]</script>
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index d43deb02ce983e58ae8381f67864f68a605f2c1b..122e163068e3abb475f7f79e3f66bd781ffc13f8 100644
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.el-alert__description{color:var(--el-color-info)}.el-alert--info.is-dark{background-color:var(--el-color-info);color:var(--el-color-white)}.el-alert--warning{--el-alert-bg-color:var(--el-color-warning-light-9)}.el-alert--warning.is-light{background-color:var(--el-alert-bg-color)}.el-alert--warning.is-light,.el-alert--warning.is-light .el-alert__description{color:var(--el-color-warning)}.el-alert--warning.is-dark{background-color:var(--el-color-warning);color:var(--el-color-white)}.el-alert--error{--el-alert-bg-color:var(--el-color-error-light-9)}.el-alert--error.is-light{background-color:var(--el-alert-bg-color)}.el-alert--error.is-light,.el-alert--error.is-light .el-alert__description{color:var(--el-color-error)}.el-alert--error.is-dark{background-color:var(--el-color-error);color:var(--el-color-white)}.el-alert__content{display:flex;flex-direction:column;gap:4px}.el-alert .el-alert__icon{font-size:var(--el-alert-icon-size);margin-right:8px;width:var(--el-alert-icon-size)}.el-alert .el-alert__icon.is-big{font-size:var(--el-alert-icon-large-size);margin-right:12px;width:var(--el-alert-icon-large-size)}.el-alert__title{font-size:var(--el-alert-title-font-size);line-height:24px}.el-alert__title.with-description{font-size:var(--el-alert-title-with-description-font-size)}.el-alert .el-alert__description{font-size:var(--el-alert-description-font-size);margin:0}.el-alert .el-alert__close-btn{cursor:pointer;font-size:var(--el-alert-close-font-size);opacity:1;position:absolute;right:16px;top:12px}.el-alert .el-alert__close-btn.is-customed{font-size:var(--el-alert-close-customed-font-size);font-style:normal;line-height:24px;top:8px}.el-alert-fade-enter-from,.el-alert-fade-leave-active{opacity:0}.el-aside{box-sizing:border-box;flex-shrink:0;overflow:auto;width:var(--el-aside-width,300px)}.el-autocomplete{--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;display:inline-block;position:relative;width:var(--el-input-width)}.el-autocomplete__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-autocomplete__popper.el-popper,.el-autocomplete__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-border-color-light)}.el-autocomplete__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-autocomplete__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-autocomplete__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-autocomplete__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-autocomplete-suggestion{border-radius:var(--el-border-radius-base);box-sizing:border-box}.el-autocomplete-suggestion__wrap{box-sizing:border-box;max-height:280px;padding:10px 0}.el-autocomplete-suggestion__list{margin:0;padding:0}.el-autocomplete-suggestion li{color:var(--el-text-color-regular);cursor:pointer;font-size:var(--el-font-size-base);line-height:34px;list-style:none;margin:0;overflow:hidden;padding:0 20px;text-align:left;text-overflow:ellipsis;white-space:nowrap}.el-autocomplete-suggestion li.highlighted,.el-autocomplete-suggestion li:hover{background-color:var(--el-fill-color-light)}.el-autocomplete-suggestion li.divider{border-top:1px solid var(--el-color-black);margin-top:6px}.el-autocomplete-suggestion li.divider:last-child{margin-bottom:-6px}.el-autocomplete-suggestion.is-loading li{color:var(--el-text-color-secondary);font-size:20px;height:100px;line-height:100px;text-align:center}.el-autocomplete-suggestion.is-loading li:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-autocomplete-suggestion.is-loading li:hover{background-color:var(--el-bg-color-overlay)}.el-autocomplete-suggestion.is-loading .el-icon-loading{vertical-align:middle}.el-avatar{--el-avatar-text-color:var(--el-color-white);--el-avatar-bg-color:var(--el-text-color-disabled);--el-avatar-text-size:14px;--el-avatar-icon-size:18px;--el-avatar-border-radius:var(--el-border-radius-base);--el-avatar-size-large:56px;--el-avatar-size-small:24px;--el-avatar-size:40px;align-items:center;background:var(--el-avatar-bg-color);box-sizing:border-box;color:var(--el-avatar-text-color);display:inline-flex;font-size:var(--el-avatar-text-size);height:var(--el-avatar-size);justify-content:center;outline:none;overflow:hidden;text-align:center;width:var(--el-avatar-size)}.el-avatar>img{display:block;height:100%;width:100%}.el-avatar--circle{border-radius:50%}.el-avatar--square{border-radius:var(--el-avatar-border-radius)}.el-avatar--icon{font-size:var(--el-avatar-icon-size)}.el-avatar--small{--el-avatar-size:24px}.el-avatar--large{--el-avatar-size:56px}.el-backtop{--el-backtop-bg-color:var(--el-bg-color-overlay);--el-backtop-text-color:var(--el-color-primary);--el-backtop-hover-bg-color:var(--el-border-color-extra-light);align-items:center;background-color:var(--el-backtop-bg-color);border-radius:50%;box-shadow:var(--el-box-shadow-lighter);color:var(--el-backtop-text-color);cursor:pointer;display:flex;font-size:20px;height:40px;justify-content:center;position:fixed;width:40px;z-index:5}.el-backtop:hover{background-color:var(--el-backtop-hover-bg-color)}.el-backtop__icon{font-size:20px}.el-badge{--el-badge-bg-color:var(--el-color-danger);--el-badge-radius:10px;--el-badge-font-size:12px;--el-badge-padding:6px;--el-badge-size:18px;display:inline-block;position:relative;vertical-align:middle;width:-moz-fit-content;width:fit-content}.el-badge__content{align-items:center;background-color:var(--el-badge-bg-color);border:1px solid var(--el-bg-color);border-radius:var(--el-badge-radius);color:var(--el-color-white);display:inline-flex;font-size:var(--el-badge-font-size);height:var(--el-badge-size);justify-content:center;padding:0 var(--el-badge-padding);white-space:nowrap}.el-badge__content.is-fixed{position:absolute;right:calc(1px + var(--el-badge-size)/2);top:0;transform:translateY(-50%) translateX(100%);z-index:var(--el-index-normal)}.el-badge__content.is-fixed.is-dot{right:5px}.el-badge__content.is-dot{border-radius:50%;height:8px;padding:0;right:0;width:8px}.el-badge__content--primary{background-color:var(--el-color-primary)}.el-badge__content--success{background-color:var(--el-color-success)}.el-badge__content--warning{background-color:var(--el-color-warning)}.el-badge__content--info{background-color:var(--el-color-info)}.el-badge__content--danger{background-color:var(--el-color-danger)}.el-breadcrumb{font-size:14px;line-height:1}.el-breadcrumb:after,.el-breadcrumb:before{content:"";display:table}.el-breadcrumb:after{clear:both}.el-breadcrumb__separator{color:var(--el-text-color-placeholder);font-weight:700;margin:0 9px}.el-breadcrumb__separator.el-icon{font-weight:400;margin:0 6px}.el-breadcrumb__separator.el-icon svg{vertical-align:middle}.el-breadcrumb__item{align-items:center;display:inline-flex;float:left}.el-breadcrumb__inner{color:var(--el-text-color-regular)}.el-breadcrumb__inner a,.el-breadcrumb__inner.is-link{color:var(--el-text-color-primary);font-weight:700;text-decoration:none;transition:var(--el-transition-color)}.el-breadcrumb__inner a:hover,.el-breadcrumb__inner.is-link:hover{color:var(--el-color-primary);cursor:pointer}.el-breadcrumb__item:last-child .el-breadcrumb__inner,.el-breadcrumb__item:last-child .el-breadcrumb__inner a,.el-breadcrumb__item:last-child .el-breadcrumb__inner a:hover,.el-breadcrumb__item:last-child .el-breadcrumb__inner:hover{color:var(--el-text-color-regular);cursor:text;font-weight:400}.el-breadcrumb__item:last-child .el-breadcrumb__separator{display:none}.el-button-group{display:inline-block;vertical-align:middle}.el-button-group:after,.el-button-group:before{content:"";display:table}.el-button-group:after{clear:both}.el-button-group>.el-button{float:left;position:relative}.el-button-group>.el-button+.el-button{margin-left:0}.el-button-group>.el-button:first-child{border-bottom-right-radius:0;border-top-right-radius:0}.el-button-group>.el-button:last-child{border-bottom-left-radius:0;border-top-left-radius:0}.el-button-group>.el-button:first-child:last-child{border-bottom-left-radius:var(--el-border-radius-base);border-bottom-right-radius:var(--el-border-radius-base);border-top-left-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-button-group>.el-button:first-child:last-child.is-round{border-radius:var(--el-border-radius-round)}.el-button-group>.el-button:first-child:last-child.is-circle{border-radius:50%}.el-button-group>.el-button:not(:first-child):not(:last-child){border-radius:0}.el-button-group>.el-button:not(:last-child){margin-right:-1px}.el-button-group>.el-button.is-active,.el-button-group>.el-button:active,.el-button-group>.el-button:focus,.el-button-group>.el-button:hover{z-index:1}.el-button-group>.el-dropdown>.el-button{border-bottom-left-radius:0;border-left-color:var(--el-button-divide-border-color);border-top-left-radius:0}.el-button-group .el-button--primary:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--primary:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--primary:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--success:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--success:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--success:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--warning:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--warning:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--warning:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--danger:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--danger:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--danger:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--info:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--info:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--info:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button{--el-button-font-weight:var(--el-font-weight-primary);--el-button-border-color:var(--el-border-color);--el-button-bg-color:var(--el-fill-color-blank);--el-button-text-color:var(--el-text-color-regular);--el-button-disabled-text-color:var(--el-disabled-text-color);--el-button-disabled-bg-color:var(--el-fill-color-blank);--el-button-disabled-border-color:var(--el-border-color-light);--el-button-divide-border-color:hsla(0,0%,100%,.5);--el-button-hover-text-color:var(--el-color-primary);--el-button-hover-bg-color:var(--el-color-primary-light-9);--el-button-hover-border-color:var(--el-color-primary-light-7);--el-button-active-text-color:var(--el-button-hover-text-color);--el-button-active-border-color:var(--el-color-primary);--el-button-active-bg-color:var(--el-button-hover-bg-color);--el-button-outline-color:var(--el-color-primary-light-5);--el-button-hover-link-text-color:var(--el-color-info);--el-button-active-color:var(--el-text-color-primary);align-items:center;-webkit-appearance:none;background-color:var(--el-button-bg-color);border:var(--el-border);border-color:var(--el-button-border-color);box-sizing:border-box;color:var(--el-button-text-color);cursor:pointer;display:inline-flex;font-weight:var(--el-button-font-weight);height:32px;justify-content:center;line-height:1;outline:none;text-align:center;transition:.1s;-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-button:hover{background-color:var(--el-button-hover-bg-color);border-color:var(--el-button-hover-border-color);color:var(--el-button-hover-text-color);outline:none}.el-button:active{background-color:var(--el-button-active-bg-color);border-color:var(--el-button-active-border-color);color:var(--el-button-active-text-color);outline:none}.el-button:focus-visible{outline:2px solid var(--el-button-outline-color);outline-offset:1px;transition:outline-offset 0s,outline 0s}.el-button>span{align-items:center;display:inline-flex}.el-button+.el-button{margin-left:12px}.el-button{border-radius:var(--el-border-radius-base);font-size:var(--el-font-size-base)}.el-button,.el-button.is-round{padding:8px 15px}.el-button::-moz-focus-inner{border:0}.el-button [class*=el-icon]+span{margin-left:6px}.el-button [class*=el-icon] 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20px;position:relative}.el-cascader-node.is-selectable.in-active-path{color:var(--el-cascader-menu-text-color)}.el-cascader-node.in-active-path,.el-cascader-node.is-active,.el-cascader-node.is-selectable.in-checked-path{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader-node:not(.is-disabled){cursor:pointer}.el-cascader-node:not(.is-disabled):focus,.el-cascader-node:not(.is-disabled):hover{background:var(--el-cascader-node-background-hover)}.el-cascader-node.is-disabled{color:var(--el-cascader-node-color-disabled);cursor:not-allowed}.el-cascader-node__prefix{left:10px;position:absolute}.el-cascader-node__postfix{position:absolute;right:10px}.el-cascader-node__label{flex:1;overflow:hidden;padding:0 8px;text-align:left;text-overflow:ellipsis;white-space:nowrap}.el-cascader-node>.el-checkbox,.el-cascader-node>.el-radio{margin-right:0}.el-cascader-node>.el-radio .el-radio__label{padding-left:0}.el-cascader{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);display:inline-block;font-size:var(--el-font-size-base);line-height:32px;outline:none;position:relative;vertical-align:middle}.el-cascader:not(.is-disabled):hover .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset;cursor:pointer}.el-cascader .el-input{cursor:pointer;display:flex}.el-cascader .el-input .el-input__inner{cursor:pointer;text-overflow:ellipsis}.el-cascader .el-input .el-input__suffix-inner .el-icon{height:calc(100% - 2px)}.el-cascader .el-input .el-input__suffix-inner .el-icon svg{vertical-align:middle}.el-cascader .el-input .icon-arrow-down{font-size:14px;transition:transform var(--el-transition-duration)}.el-cascader .el-input .icon-arrow-down.is-reverse{transform:rotate(180deg)}.el-cascader .el-input .icon-circle-close:hover{color:var(--el-input-clear-hover-color,var(--el-text-color-secondary))}.el-cascader .el-input.is-focus .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-focus-border-color,var(--el-color-primary)) inset}.el-cascader--large{font-size:14px;line-height:40px}.el-cascader--small{font-size:12px;line-height:24px}.el-cascader.is-disabled .el-cascader__label{color:var(--el-disabled-text-color);z-index:calc(var(--el-index-normal) + 1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper .el-popper__arrow:before{border:var(--el-cascader-menu-border)}.el-cascader__dropdown.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper{box-shadow:var(--el-cascader-menu-shadow)}.el-cascader__tags{box-sizing:border-box;display:flex;flex-wrap:wrap;left:0;line-height:normal;position:absolute;right:30px;text-align:left;top:50%;transform:translateY(-50%)}.el-cascader__tags .el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags .el-tag{align-items:center;background:var(--el-fill-color);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag.el-tag--dark,.el-cascader__collapse-tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__collapse-tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__collapse-tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__collapse-tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__suggestion-panel{border-radius:var(--el-cascader-menu-radius)}.el-cascader__suggestion-list{color:var(--el-cascader-menu-text-color);font-size:var(--el-font-size-base);margin:0;max-height:204px;padding:6px 0;text-align:center}.el-cascader__suggestion-item{align-items:center;cursor:pointer;display:flex;height:34px;justify-content:space-between;outline:none;padding:0 15px;text-align:left}.el-cascader__suggestion-item:focus,.el-cascader__suggestion-item:hover{background:var(--el-cascader-node-background-hover)}.el-cascader__suggestion-item.is-checked{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader__suggestion-item>span{margin-right:10px}.el-cascader__empty-text{color:var(--el-cascader-color-empty);margin:10px 0}.el-cascader__search-input{background:transparent;border:none;box-sizing:border-box;color:var(--el-cascader-menu-text-color);flex:1;height:24px;margin:2px 0 2px 11px;min-width:60px;outline:none;padding:0}.el-cascader__search-input::-moz-placeholder{color:transparent}.el-cascader__search-input::placeholder{color:transparent}.el-check-tag{background-color:var(--el-color-info-light-9);border-radius:var(--el-border-radius-base);color:var(--el-color-info);cursor:pointer;display:inline-block;font-size:var(--el-font-size-base);font-weight:700;line-height:var(--el-font-size-base);padding:7px 15px;transition:var(--el-transition-all)}.el-check-tag:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--primary.is-checked{background-color:var(--el-color-primary-light-8);color:var(--el-color-primary)}.el-check-tag.el-check-tag--primary.is-checked:hover{background-color:var(--el-color-primary-light-7)}.el-check-tag.el-check-tag--primary.is-checked.is-disabled{background-color:var(--el-color-primary-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-checked.is-disabled:hover{background-color:var(--el-color-primary-light-8)}.el-check-tag.el-check-tag--primary.is-disabled{background-color:var(--el-color-info-light-9);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-check-tag.el-check-tag--success.is-checked{background-color:var(--el-color-success-light-8);color:var(--el-color-success)}.el-check-tag.el-check-tag--success.is-checked:hover{background-color:var(--el-color-success-light-7)}.el-check-tag.el-check-tag--success.is-checked.is-disabled{background-color:var(--el-color-success-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-checked.is-disabled:hover{background-color:var(--el-color-success-light-8)}.el-check-tag.el-check-tag--success.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-disabled,.el-check-tag.el-check-tag--success.is-disabled:hover{background-color:var(--el-color-success-light-9)}.el-check-tag.el-check-tag--warning.is-checked{background-color:var(--el-color-warning-light-8);color:var(--el-color-warning)}.el-check-tag.el-check-tag--warning.is-checked:hover{background-color:var(--el-color-warning-light-7)}.el-check-tag.el-check-tag--warning.is-checked.is-disabled{background-color:var(--el-color-warning-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-checked.is-disabled:hover{background-color:var(--el-color-warning-light-8)}.el-check-tag.el-check-tag--warning.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-disabled,.el-check-tag.el-check-tag--warning.is-disabled:hover{background-color:var(--el-color-warning-light-9)}.el-check-tag.el-check-tag--danger.is-checked{background-color:var(--el-color-danger-light-8);color:var(--el-color-danger)}.el-check-tag.el-check-tag--danger.is-checked:hover{background-color:var(--el-color-danger-light-7)}.el-check-tag.el-check-tag--danger.is-checked.is-disabled{background-color:var(--el-color-danger-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-checked.is-disabled:hover{background-color:var(--el-color-danger-light-8)}.el-check-tag.el-check-tag--danger.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-disabled,.el-check-tag.el-check-tag--danger.is-disabled:hover{background-color:var(--el-color-danger-light-9)}.el-check-tag.el-check-tag--error.is-checked{background-color:var(--el-color-error-light-8);color:var(--el-color-error)}.el-check-tag.el-check-tag--error.is-checked:hover{background-color:var(--el-color-error-light-7)}.el-check-tag.el-check-tag--error.is-checked.is-disabled{background-color:var(--el-color-error-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-checked.is-disabled:hover{background-color:var(--el-color-error-light-8)}.el-check-tag.el-check-tag--error.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-disabled,.el-check-tag.el-check-tag--error.is-disabled:hover{background-color:var(--el-color-error-light-9)}.el-check-tag.el-check-tag--info.is-checked{background-color:var(--el-color-info-light-8);color:var(--el-color-info)}.el-check-tag.el-check-tag--info.is-checked:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--info.is-checked.is-disabled{background-color:var(--el-color-info-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-checked.is-disabled:hover{background-color:var(--el-color-info-light-8)}.el-check-tag.el-check-tag--info.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-disabled,.el-check-tag.el-check-tag--info.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-checkbox-button{--el-checkbox-button-checked-bg-color:var(--el-color-primary);--el-checkbox-button-checked-text-color:var(--el-color-white);--el-checkbox-button-checked-border-color:var(--el-color-primary);display:inline-block;position:relative}.el-checkbox-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left-color:transparent;bord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15px;position:relative;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-checkbox-button__inner.is-round{padding:8px 15px}.el-checkbox-button__inner:hover{color:var(--el-color-primary)}.el-checkbox-button__inner [class*=el-icon-]{line-height:.9}.el-checkbox-button__inner [class*=el-icon-]+span{margin-left:5px}.el-checkbox-button__original{margin:0;opacity:0;outline:none;position:absolute;z-index:-1}.el-checkbox-button.is-checked .el-checkbox-button__inner{background-color:var(--el-checkbox-button-checked-bg-color);border-color:var(--el-checkbox-button-checked-border-color);box-shadow:-1px 0 0 0 var(--el-color-primary-light-7);color:var(--el-checkbox-button-checked-text-color)}.el-checkbox-button.is-checked:first-child .el-checkbox-button__inner{border-left-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button.is-disabled .el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large .el-checkbox-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-checkbox-button--large .el-checkbox-button__inner.is-round{padding:12px 19px}.el-checkbox-button--small .el-checkbox-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-checkbox-button--small .el-checkbox-button__inner.is-round{padding:5px 11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) .el-checkbox__original:not(:focus-visible){border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__input.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-checked-icon-color);content:"";display:block;height:2px;left:0;position:absolute;right:0;top:5px;transform:scale(.5)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:after{display:none}.el-checkbox__inner{background-color:var(--el-checkbox-bg-color);border:var(--el-checkbox-input-border);border-radius:var(--el-checkbox-border-radius);box-sizing:border-box;display:inline-block;height:var(--el-checkbox-input-height);position:relative;transition:border-color .25s cubic-bezier(.71,-.46,.29,1.46),background-color .25s cubic-bezier(.71,-.46,.29,1.46),outline .25s cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 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54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 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var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid 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var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker .el-picker-panel__content{margin:0}.el-date-range-picker__header{height:28px;position:relative;text-align:center}.el-date-range-picker__header [class*=arrow-left]{float:left}.el-date-range-picker__header [class*=arrow-right]{float:right}.el-date-range-picker__header div{font-size:16px;font-weight:500;margin-right:50px}.el-date-range-picker__content{box-sizing:border-box;float:left;margin:0;padding:16px;width:50%}.el-date-range-picker__content.is-left{border-right:1px solid var(--el-datepicker-inner-border-color)}.el-date-range-picker__content .el-date-range-picker__header div{margin-left:50px;margin-right:50px}.el-date-range-picker__editors-wrap{box-sizing:border-box;display:table-cell}.el-date-range-picker__editors-wrap.is-right{text-align:right}.el-date-range-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid var(--el-timepicker-inner-border-color,var(--el-border-color-light));box-sizing:border-box;height:36px;line-height:25px;padding:4px;text-align:right}.el-time-panel__btn{background-color:transparent;border:none;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:28px;margin:0 5px;outline:none;padding:0 5px}.el-time-panel__btn.confirm{color:var(--el-timepicker-active-color,var(--el-color-primary));font-weight:800}.el-descriptions{--el-descriptions-table-border:1px solid var(--el-border-color-lighter);--el-descriptions-item-bordered-label-background:var(--el-fill-color-light);box-sizing:border-box;color:var(--el-text-color-primary);font-size:var(--el-font-size-base)}.el-descriptions__header{align-items:center;display:flex;justify-content:space-between;margin-bottom:16px}.el-descriptions__title{color:var(--el-text-color-primary);font-size:16px;font-weight:700}.el-descriptions__body{background-color:var(--el-fill-color-blank)}.el-descriptions__body 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var(--el-menu-base-level-padding)}.horizontal-collapse-transition .el-sub-menu__title 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.el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small .el-radio__inner{height:12px;width:12px}.el-rate{--el-rate-height:20px;--el-rate-font-size:var(--el-font-size-base);--el-rate-icon-size:18px;--el-rate-icon-margin:6px;--el-rate-void-color:var(--el-border-color-darker);--el-rate-fill-color:#f7ba2a;--el-rate-disabled-void-color:var(--el-fill-color);--el-rate-text-color:var(--el-text-color-primary);align-items:center;display:inline-flex;height:32px}.el-rate:active,.el-rate:focus{outline:none}.el-rate__item{color:var(--el-rate-void-color);cursor:pointer;display:inline-block;font-size:0;line-height:normal;position:relative;vertical-align:middle}.el-rate .el-rate__icon{display:inline-block;font-size:var(--el-rate-icon-size);margin-right:var(--el-rate-icon-margin);position:relative;transition:var(--el-transition-duration)}.el-rate .el-rate__icon.hover{transform:scale(1.15)}.el-rate .el-rate__icon .path2{left:0;position:absolute;top:0}.el-rate .el-rate__icon.is-active{color:var(--el-rate-fill-color)}.el-rate__decimal{color:var(--el-rate-fill-color);display:inline-block;overflow:hidden}.el-rate__decimal,.el-rate__decimal--box{left:0;position:absolute;top:0}.el-rate__text{color:var(--el-rate-text-color);font-size:var(--el-rate-font-size);vertical-align:middle}.el-rate--large{height:40px}.el-rate--small{height:24px}.el-rate--small .el-rate__icon{font-size:14px}.el-rate.is-disabled .el-rate__item{color:var(--el-rate-disabled-void-color);cursor:auto}.el-result{--el-result-padding:40px 30px;--el-result-icon-font-size:64px;--el-result-title-font-size:20px;--el-result-title-margin-top:20px;--el-result-subtitle-margin-top:10px;--el-result-extra-margin-top:30px;align-items:center;box-sizing:border-box;display:flex;flex-direction:column;justify-content:center;padding:var(--el-result-padding);text-align:center}.el-result__icon svg{height:var(--el-result-icon-font-size);width:var(--el-result-icon-font-size)}.el-result__title{margin-top:var(--el-result-title-margin-top)}.el-result__title p{color:var(--el-text-color-primary);font-size:var(--el-result-title-font-size);line-height:1.3;margin:0}.el-result__subtitle{margin-top:var(--el-result-subtitle-margin-top)}.el-result__subtitle p{color:var(--el-text-color-regular);font-size:var(--el-font-size-base);line-height:1.3;margin:0}.el-result__extra{margin-top:var(--el-result-extra-margin-top)}.el-result .icon-primary{--el-result-color:var(--el-color-primary);color:var(--el-result-color)}.el-result .icon-success{--el-result-color:var(--el-color-success);color:var(--el-result-color)}.el-result .icon-warning{--el-result-color:var(--el-color-warning);color:var(--el-result-color)}.el-result .icon-danger{--el-result-color:var(--el-color-danger);color:var(--el-result-color)}.el-result .icon-error{--el-result-color:var(--el-color-error);color:var(--el-result-color)}.el-result 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.el-tag__close{color:var(--el-tag-text-color);flex-shrink:0}.el-tag .el-tag__close:hover{background-color:var(--el-tag-hover-color);color:var(--el-color-white)}.el-tag.el-tag--primary{--el-tag-text-color:var(--el-color-primary)}.el-tag.el-tag--success{--el-tag-text-color:var(--el-color-success)}.el-tag.el-tag--warning{--el-tag-text-color:var(--el-color-warning)}.el-tag.el-tag--danger{--el-tag-text-color:var(--el-color-danger)}.el-tag.el-tag--error{--el-tag-text-color:var(--el-color-error)}.el-tag.el-tag--info{--el-tag-text-color:var(--el-color-info)}.el-tag .el-icon{border-radius:50%;cursor:pointer;font-size:calc(var(--el-icon-size) - 2px);height:var(--el-icon-size);width:var(--el-icon-size)}.el-tag 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0;min-width:0}.time-select .el-picker-panel__content{margin:0;max-height:200px}.time-select-item{font-size:14px;line-height:20px;padding:8px 10px}.time-select-item.disabled{color:var(--el-datepicker-border-color);cursor:not-allowed}.time-select-item:hover{background-color:var(--el-fill-color-light);cursor:pointer;font-weight:700}.time-select .time-select-item.selected:not(.disabled){color:var(--el-color-primary);font-weight:700}.el-timeline-item{padding-bottom:20px;position:relative}.el-timeline-item__wrapper{padding-left:28px;position:relative;top:-3px}.el-timeline-item__tail{border-left:2px solid var(--el-timeline-node-color);height:100%;left:4px;position:absolute}.el-timeline-item .el-timeline-item__icon{color:var(--el-color-white);font-size:var(--el-font-size-small)}.el-timeline-item__node{align-items:center;background-color:var(--el-timeline-node-color);border-color:var(--el-timeline-node-color);border-radius:50%;box-sizing:border-box;display:flex;justify-content:center;position:absolute}.el-timeline-item__node--normal{height:var(--el-timeline-node-size-normal);left:-1px;width:var(--el-timeline-node-size-normal)}.el-timeline-item__node--large{height:var(--el-timeline-node-size-large);left:-2px;width:var(--el-timeline-node-size-large)}.el-timeline-item__node.is-hollow{background:var(--el-color-white);border-style:solid;border-width:2px}.el-timeline-item__node--primary{background-color:var(--el-color-primary);border-color:var(--el-color-primary)}.el-timeline-item__node--success{background-color:var(--el-color-success);border-color:var(--el-color-success)}.el-timeline-item__node--warning{background-color:var(--el-color-warning);border-color:var(--el-color-warning)}.el-timeline-item__node--danger{background-color:var(--el-color-danger);border-color:var(--el-color-danger)}.el-timeline-item__node--info{background-color:var(--el-color-info);border-color:var(--el-color-info)}.el-timeline-item__dot{align-items:center;display:flex;justify-content:center;position:absolute}.el-timeline-item__content{color:var(--el-text-color-primary)}.el-timeline-item__timestamp{color:var(--el-text-color-secondary);font-size:var(--el-font-size-small);line-height:1}.el-timeline-item__timestamp.is-top{margin-bottom:8px;padding-top:4px}.el-timeline-item__timestamp.is-bottom{margin-top:8px}.el-timeline{--el-timeline-node-size-normal:12px;--el-timeline-node-size-large:14px;--el-timeline-node-color:var(--el-border-color-light);font-size:var(--el-font-size-base);list-style:none;margin:0}.el-timeline 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flex flex-wrap flex-col items-end p-10"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/home-title.png 1x, https://static.igem.wiki/teams/5358/banner/home-title.png 2x" class="w-[45vw] block" src="https://static.igem.wiki/teams/5358/banner/home-title.png"><div class="w-[25vw] flex mt-12 items-center font-semibold text-2xl"><span>BIT</span><span class="grow bg-white h-[1px] mx-5"></span><span>2024</span></div></div><div class="indicator-icon-group flex flex-col items-center justify-center mt-8"><span class="text-[#AEDE44] font-bold text-lg"> Scroll to Backgrounds </span><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" width="25" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/imgs/arrow-index.svg?width=25 1x, https://static.igem.wiki/teams/5358/imgs/arrow-index.svg?width=50 2x" class="mt-2" src="https://static.igem.wiki/teams/5358/imgs/arrow-index.svg?width=25"></div><div><div class="scrolling-animation m-20"><h2 data-aos="fade-up" data-aos-duration="1000"><p>&quot;There was a strange stillness. The birds, for example — where had they gone? Many people spoke of them, puzzled and disturbed. The feeding stations in the backyards were deserted. The few birds seen anywhere were moribund; they trembled violently and could not fly. It was a spring without voices, only silence lay over the fields, woods, and marsh.&quot;</p><p class="text-right mt-3">——*Silent Spring* written by Rachel Carson</p></h2><div class="my-20 h-[1000vh]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/border.webp 1x, https://static.igem.wiki/teams/5358/home/border.webp 2x" class="block px-10 sticky top-[20vh] z-[3]" data-aos="fade-up" data-aos-duration="500" data-aos-offset="0" src="https://static.igem.wiki/teams/5358/home/border.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p1-bg.webp 1x, https://static.igem.wiki/teams/5358/home/p1-bg.webp 2x" class="block px-10 sticky top-[20vh] z-[2]" data-aos="fade-up" data-aos-duration="500" data-aos-offset="500" src="https://static.igem.wiki/teams/5358/home/p1-bg.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p1-man.webp 1x, https://static.igem.wiki/teams/5358/home/p1-man.webp 2x" class="block px-10 sticky top-[20vh] z-[1]" data-aos="fade-right" data-aos-duration="500" data-aos-offset="1000" src="https://static.igem.wiki/teams/5358/home/p1-man.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p1-cloud.webp 1x, https://static.igem.wiki/teams/5358/home/p1-cloud.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-left" data-aos-duration="500" data-aos-offset="1500" src="https://static.igem.wiki/teams/5358/home/p1-cloud.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p1-tofall-bird.webp 1x, https://static.igem.wiki/teams/5358/home/p1-tofall-bird.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-left" data-aos-duration="200" data-aos-offset="1700" src="https://static.igem.wiki/teams/5358/home/p1-tofall-bird.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p1-falling-bird.webp 1x, https://static.igem.wiki/teams/5358/home/p1-falling-bird.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-zoom-in" data-aos-duration="200" data-aos-offset="1900" src="https://static.igem.wiki/teams/5358/home/p1-falling-bird.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p1-falldown-bird.webp 1x, https://static.igem.wiki/teams/5358/home/p1-falldown-bird.webp 2x" class="block px-10 sticky top-[20vh] z-[3]" data-aos="fade-zoom-in" data-aos-duration="200" data-aos-offset="2100" src="https://static.igem.wiki/teams/5358/home/p1-falldown-bird.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p1-eyedrop.webp 1x, https://static.igem.wiki/teams/5358/home/p1-eyedrop.webp 2x" class="block px-10 sticky top-[20vh] z-[3]" data-aos="fade-zoom-in" data-aos-duration="100" data-aos-offset="2200" src="https://static.igem.wiki/teams/5358/home/p1-eyedrop.webp"></div><h2 data-aos="fade-up" data-aos-duration="1000" class="sticky"><p class="text-right w-[60vw] ml-auto text-balance">&quot;Silent Spring&quot; depicts the environmental pollution and ecological damage caused by excessive use of chemicals and fertilizers, ultimately leading to unbearable disasters for humanity.</p><p class="text-right mt-10 w-[60vw] ml-auto text-balance">Are you worried that the phenomena described in the book will become a reality? </p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left"> After the publication of the book, people were surprised to find that the words written in it were turning into reality. A series of strange and inexplicable phenomena began to appear all over the world. Many reports about animals and humans are worrying: abnormal behavior, decreased reproductive capacity, and death of young. </p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left"> A dangerous class of chemicals is harming environmental health - environmental endocrine disruptors: (refers to exogenous chemical substances released into the environment by human production and life that affect the normal hormone levels in the bodies of humans and animals.) </p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left">Many familiar pesticides and material additives belong to environmental endocrine disruptors, and in fact, there are even more endocrine disruptors lurking around us...</p></h2><div class="my-20 h-[800vh]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/border.webp 1x, https://static.igem.wiki/teams/5358/home/border.webp 2x" class="block px-10 sticky top-[20vh] z-[3]" data-aos="fade-down" data-aos-duration="400" data-aos-offset="0" src="https://static.igem.wiki/teams/5358/home/border.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p2-cloud.webp 1x, https://static.igem.wiki/teams/5358/home/p2-cloud.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-down" data-aos-duration="100" data-aos-offset="400" src="https://static.igem.wiki/teams/5358/home/p2-cloud.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p2-bg.webp 1x, https://static.igem.wiki/teams/5358/home/p2-bg.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-up" data-aos-duration="500" data-aos-offset="300" src="https://static.igem.wiki/teams/5358/home/p2-bg.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p2-moon.webp 1x, https://static.igem.wiki/teams/5358/home/p2-moon.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-down" data-aos-duration="200" data-aos-offset="800" src="https://static.igem.wiki/teams/5358/home/p2-moon.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p2-death.webp 1x, https://static.igem.wiki/teams/5358/home/p2-death.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-down" data-aos-duration="200" data-aos-offset="1000" src="https://static.igem.wiki/teams/5358/home/p2-death.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p2-hand.webp 1x, https://static.igem.wiki/teams/5358/home/p2-hand.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-zoom-in" data-aos-duration="200" data-aos-offset="1200" src="https://static.igem.wiki/teams/5358/home/p2-hand.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p2-drug.webp 1x, https://static.igem.wiki/teams/5358/home/p2-drug.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-zoom-in" data-aos-duration="200" data-aos-offset="1400" src="https://static.igem.wiki/teams/5358/home/p2-drug.webp"></div><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left text-3xl font-extrabold 2xl:text-5xl">And it is our task to find out these substances.</p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left">The important mechanism of action of environmental endocrine disruptors in living organisms is to bind to hormone receptors, thereby disrupting the regulation of hormones. We will start from this binding effect to find a way to detect the endocrine disruptor effect.</p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left text-3xl font-extrabold 2xl:text-5xl">But that is not enough,</p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left">The action of environmental endocrine disruptors in living organisms is complex and involves multiple effects. Therefore, we will use the HRP and luxR/I system to construct an AND gate detection circuit and combine it with other toxic effects to achieve a more comprehensive and detailed detection and evaluation of environmental endocrine disruptors.</p></h2><div class="my-20 h-[400vh]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/border.webp 1x, https://static.igem.wiki/teams/5358/home/border.webp 2x" class="block px-10 sticky top-[20vh] z-[3] rotate-180" data-aos="fade-up" data-aos-duration="500" data-aos-offset="0" src="https://static.igem.wiki/teams/5358/home/border.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p3-bg.webp 1x, https://static.igem.wiki/teams/5358/home/p3-bg.webp 2x" class="block px-10 sticky top-[20vh] rotate-180" data-aos="fade-up" data-aos-duration="500" data-aos-offset="500" src="https://static.igem.wiki/teams/5358/home/p3-bg.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p3-bacteria.webp 1x, https://static.igem.wiki/teams/5358/home/p3-bacteria.webp 2x" class="block px-10 sticky top-[20vh] rotate-180" data-aos="fade-right" data-aos-duration="500" data-aos-offset="1000" src="https://static.igem.wiki/teams/5358/home/p3-bacteria.webp"></div><div class="text-right"><div class="mt-20 text-right font-bold text-2xl hover:border-emerald-200 border-2 inline-block rounded-xl p-2 hover:text-emerald-300"><a href="/bit/description" class=""> For more information, see Description ... </a></div></div></div></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":11,"prerenderedAt":12},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{},"/",1727868994393]</script>
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flex flex-wrap flex-col items-end p-10"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/home-title.png 1x, https://static.igem.wiki/teams/5358/banner/home-title.png 2x" class="w-[45vw] block" src="https://static.igem.wiki/teams/5358/banner/home-title.png"><div class="w-[25vw] flex mt-12 items-center font-semibold text-2xl"><span>BIT</span><span class="grow bg-white h-[1px] mx-5"></span><span>2024</span></div></div><div class="indicator-icon-group flex flex-col items-center justify-center mt-8"><span class="text-[#AEDE44] font-bold text-lg"> Scroll to Backgrounds </span><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" width="25" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/imgs/arrow-index.svg?width=25 1x, https://static.igem.wiki/teams/5358/imgs/arrow-index.svg?width=50 2x" class="mt-2" src="https://static.igem.wiki/teams/5358/imgs/arrow-index.svg?width=25"></div><div><div class="scrolling-animation m-20"><h2 data-aos="fade-up" data-aos-duration="1000"><p>&quot;There was a strange stillness. The birds, for example — where had they gone? Many people spoke of them, puzzled and disturbed. The feeding stations in the backyards were deserted. The few birds seen anywhere were moribund; they trembled violently and could not fly. It was a spring without voices, only silence lay over the fields, woods, and marsh.&quot;</p><p class="text-right mt-3">——*Silent Spring* written by Rachel Carson</p></h2><div class="my-20 h-[1000vh]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/border.webp 1x, https://static.igem.wiki/teams/5358/home/border.webp 2x" class="block px-10 sticky top-[20vh] z-[3]" data-aos="fade-up" data-aos-duration="500" data-aos-offset="0" src="https://static.igem.wiki/teams/5358/home/border.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p1-bg.webp 1x, https://static.igem.wiki/teams/5358/home/p1-bg.webp 2x" class="block px-10 sticky top-[20vh] z-[2]" data-aos="fade-up" data-aos-duration="500" data-aos-offset="500" src="https://static.igem.wiki/teams/5358/home/p1-bg.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p1-man.webp 1x, https://static.igem.wiki/teams/5358/home/p1-man.webp 2x" class="block px-10 sticky top-[20vh] z-[1]" data-aos="fade-right" data-aos-duration="500" data-aos-offset="1000" src="https://static.igem.wiki/teams/5358/home/p1-man.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p1-cloud.webp 1x, https://static.igem.wiki/teams/5358/home/p1-cloud.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-left" data-aos-duration="500" data-aos-offset="1500" src="https://static.igem.wiki/teams/5358/home/p1-cloud.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p1-tofall-bird.webp 1x, https://static.igem.wiki/teams/5358/home/p1-tofall-bird.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-left" data-aos-duration="200" data-aos-offset="1700" src="https://static.igem.wiki/teams/5358/home/p1-tofall-bird.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p1-falling-bird.webp 1x, https://static.igem.wiki/teams/5358/home/p1-falling-bird.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-zoom-in" data-aos-duration="200" data-aos-offset="1900" src="https://static.igem.wiki/teams/5358/home/p1-falling-bird.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p1-falldown-bird.webp 1x, https://static.igem.wiki/teams/5358/home/p1-falldown-bird.webp 2x" class="block px-10 sticky top-[20vh] z-[3]" data-aos="fade-zoom-in" data-aos-duration="200" data-aos-offset="2100" src="https://static.igem.wiki/teams/5358/home/p1-falldown-bird.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p1-eyedrop.webp 1x, https://static.igem.wiki/teams/5358/home/p1-eyedrop.webp 2x" class="block px-10 sticky top-[20vh] z-[3]" data-aos="fade-zoom-in" data-aos-duration="100" data-aos-offset="2200" src="https://static.igem.wiki/teams/5358/home/p1-eyedrop.webp"></div><h2 data-aos="fade-up" data-aos-duration="1000" class="sticky"><p class="text-right w-[60vw] ml-auto text-balance">&quot;Silent Spring&quot; depicts the environmental pollution and ecological damage caused by excessive use of chemicals and fertilizers, ultimately leading to unbearable disasters for humanity.</p><p class="text-right mt-10 w-[60vw] ml-auto text-balance">Are you worried that the phenomena described in the book will become a reality? </p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left"> After the publication of the book, people were surprised to find that the words written in it were turning into reality. A series of strange and inexplicable phenomena began to appear all over the world. Many reports about animals and humans are worrying: abnormal behavior, decreased reproductive capacity, and death of young. </p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left"> A dangerous class of chemicals is harming environmental health - environmental endocrine disruptors: (refers to exogenous chemical substances released into the environment by human production and life that affect the normal hormone levels in the bodies of humans and animals.) </p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left">Many familiar pesticides and material additives belong to environmental endocrine disruptors, and in fact, there are even more endocrine disruptors lurking around us...</p></h2><div class="my-20 h-[800vh]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/border.webp 1x, https://static.igem.wiki/teams/5358/home/border.webp 2x" class="block px-10 sticky top-[20vh] z-[3]" data-aos="fade-down" data-aos-duration="400" data-aos-offset="0" src="https://static.igem.wiki/teams/5358/home/border.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p2-cloud.webp 1x, https://static.igem.wiki/teams/5358/home/p2-cloud.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-down" data-aos-duration="100" data-aos-offset="400" src="https://static.igem.wiki/teams/5358/home/p2-cloud.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p2-bg.webp 1x, https://static.igem.wiki/teams/5358/home/p2-bg.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-up" data-aos-duration="500" data-aos-offset="300" src="https://static.igem.wiki/teams/5358/home/p2-bg.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p2-moon.webp 1x, https://static.igem.wiki/teams/5358/home/p2-moon.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-down" data-aos-duration="200" data-aos-offset="800" src="https://static.igem.wiki/teams/5358/home/p2-moon.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p2-death.webp 1x, https://static.igem.wiki/teams/5358/home/p2-death.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-down" data-aos-duration="200" data-aos-offset="1000" src="https://static.igem.wiki/teams/5358/home/p2-death.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p2-hand.webp 1x, https://static.igem.wiki/teams/5358/home/p2-hand.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-zoom-in" data-aos-duration="200" data-aos-offset="1200" src="https://static.igem.wiki/teams/5358/home/p2-hand.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p2-drug.webp 1x, https://static.igem.wiki/teams/5358/home/p2-drug.webp 2x" class="block px-10 sticky top-[20vh]" data-aos="fade-zoom-in" data-aos-duration="200" data-aos-offset="1400" src="https://static.igem.wiki/teams/5358/home/p2-drug.webp"></div><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left text-3xl font-extrabold 2xl:text-5xl">And it is our task to find out these substances.</p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left">The important mechanism of action of environmental endocrine disruptors in living organisms is to bind to hormone receptors, thereby disrupting the regulation of hormones. We will start from this binding effect to find a way to detect the endocrine disruptor effect.</p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left text-3xl font-extrabold 2xl:text-5xl">But that is not enough,</p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left">The action of environmental endocrine disruptors in living organisms is complex and involves multiple effects. Therefore, we will use the HRP and luxR/I system to construct an AND gate detection circuit and combine it with other toxic effects to achieve a more comprehensive and detailed detection and evaluation of environmental endocrine disruptors.</p></h2><div class="my-20 h-[400vh]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/border.webp 1x, https://static.igem.wiki/teams/5358/home/border.webp 2x" class="block px-10 sticky top-[20vh] z-[3] rotate-180" data-aos="fade-up" data-aos-duration="500" data-aos-offset="0" src="https://static.igem.wiki/teams/5358/home/border.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p3-bg.webp 1x, https://static.igem.wiki/teams/5358/home/p3-bg.webp 2x" class="block px-10 sticky top-[20vh] rotate-180" data-aos="fade-up" data-aos-duration="500" data-aos-offset="500" src="https://static.igem.wiki/teams/5358/home/p3-bg.webp"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/home/p3-bacteria.webp 1x, https://static.igem.wiki/teams/5358/home/p3-bacteria.webp 2x" class="block px-10 sticky top-[20vh] rotate-180" data-aos="fade-right" data-aos-duration="500" data-aos-offset="1000" src="https://static.igem.wiki/teams/5358/home/p3-bacteria.webp"></div><div class="text-right"><div class="mt-20 text-right font-bold text-2xl hover:border-emerald-200 border-2 inline-block rounded-xl p-2 hover:text-emerald-300"><a href="/bit/description" class=""> For more information, see Description ... </a></div></div></div></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":11,"prerenderedAt":12},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{},"/",1727871091263]</script>
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diff --git a/.output/public/member/_payload.json b/.output/public/member/_payload.json
index 59da9c7bf0139b80590e23d029a95a06f330d62f..054e23b7ed8a4c43b4ac5f309f286f59796c3a97 100644
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diff --git a/.output/public/member/index.html b/.output/public/member/index.html
index 4931a34180eb5685a4c7930eacfdf2212b7181f5..7a527cfa9f424df30505534def6c4f8bd8a09ad3 100644
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.el-button--info:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button{--el-button-font-weight:var(--el-font-weight-primary);--el-button-border-color:var(--el-border-color);--el-button-bg-color:var(--el-fill-color-blank);--el-button-text-color:var(--el-text-color-regular);--el-button-disabled-text-color:var(--el-disabled-text-color);--el-button-disabled-bg-color:var(--el-fill-color-blank);--el-button-disabled-border-color:var(--el-border-color-light);--el-button-divide-border-color:hsla(0,0%,100%,.5);--el-button-hover-text-color:var(--el-color-primary);--el-button-hover-bg-color:var(--el-color-primary-light-9);--el-button-hover-border-color:var(--el-color-primary-light-7);--el-button-active-text-color:var(--el-button-hover-text-color);--el-button-active-border-color:var(--el-color-primary);--el-button-active-bg-color:var(--el-button-hover-bg-color);--el-button-outline-color:var(--el-color-primary-light-5);--el-button-hover-link-text-color:var(--el-color-info);--el-button-active-color:var(--el-text-color-primary);align-items:center;-webkit-appearance:none;background-color:var(--el-button-bg-color);border:var(--el-border);border-color:var(--el-button-border-color);box-sizing:border-box;color:var(--el-button-text-color);cursor:pointer;display:inline-flex;font-weight:var(--el-button-font-weight);height:32px;justify-content:center;line-height:1;outline:none;text-align:center;transition:.1s;-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-button:hover{background-color:var(--el-button-hover-bg-color);border-color:var(--el-button-hover-border-color);color:var(--el-button-hover-text-color);outline:none}.el-button:active{background-color:var(--el-button-active-bg-color);border-color:var(--el-button-active-border-color);color:var(--el-button-active-text-color);outline:none}.el-button:focus-visible{outline:2px solid var(--el-button-outline-color);outline-offset:1px;transition:outline-offset 0s,outline 0s}.el-button>span{align-items:center;display:inline-flex}.el-button+.el-button{margin-left:12px}.el-button{border-radius:var(--el-border-radius-base);font-size:var(--el-font-size-base)}.el-button,.el-button.is-round{padding:8px 15px}.el-button::-moz-focus-inner{border:0}.el-button [class*=el-icon]+span{margin-left:6px}.el-button [class*=el-icon] 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1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper 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.el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large 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11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) 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cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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79.1666666667%;max-width:79.1666666667%}.el-col-xs-19.is-guttered{display:block}.el-col-xs-offset-19{margin-left:79.1666666667%}.el-col-xs-pull-19{position:relative;right:79.1666666667%}.el-col-xs-push-19{left:79.1666666667%;position:relative}.el-col-xs-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xs-20.is-guttered{display:block}.el-col-xs-offset-20{margin-left:83.3333333333%}.el-col-xs-pull-20{position:relative;right:83.3333333333%}.el-col-xs-push-20{left:83.3333333333%;position:relative}.el-col-xs-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xs-21.is-guttered{display:block}.el-col-xs-offset-21{margin-left:87.5%}.el-col-xs-pull-21{position:relative;right:87.5%}.el-col-xs-push-21{left:87.5%;position:relative}.el-col-xs-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 0%;max-width:0}.el-col-sm-0.is-guttered{display:none}.el-col-sm-offset-0{margin-left:0}.el-col-sm-pull-0{position:relative;right:0}.el-col-sm-push-0{left:0;position:relative}.el-col-sm-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-sm-1,.el-col-sm-1.is-guttered{display:block}.el-col-sm-offset-1{margin-left:4.1666666667%}.el-col-sm-pull-1{position:relative;right:4.1666666667%}.el-col-sm-push-1{left:4.1666666667%;position:relative}.el-col-sm-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-sm-2,.el-col-sm-2.is-guttered{display:block}.el-col-sm-offset-2{margin-left:8.3333333333%}.el-col-sm-pull-2{position:relative;right:8.3333333333%}.el-col-sm-push-2{left:8.3333333333%;position:relative}.el-col-sm-3{flex:0 0 12.5%;max-width:12.5%}.el-col-sm-3,.el-col-sm-3.is-guttered{display:block}.el-col-sm-offset-3{margin-left:12.5%}.el-col-sm-pull-3{position:relative;right:12.5%}.el-col-sm-push-3{left:12.5%;position:relative}.el-col-sm-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-sm-4,.el-col-sm-4.is-guttered{display:block}.el-col-sm-offset-4{margin-left:16.6666666667%}.el-col-sm-pull-4{position:relative;right:16.6666666667%}.el-col-sm-push-4{left:16.6666666667%;position:relative}.el-col-sm-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-sm-5,.el-col-sm-5.is-guttered{display:block}.el-col-sm-offset-5{margin-left:20.8333333333%}.el-col-sm-pull-5{position:relative;right:20.8333333333%}.el-col-sm-push-5{left:20.8333333333%;position:relative}.el-col-sm-6{flex:0 0 25%;max-width:25%}.el-col-sm-6,.el-col-sm-6.is-guttered{display:block}.el-col-sm-offset-6{margin-left:25%}.el-col-sm-pull-6{position:relative;right:25%}.el-col-sm-push-6{left:25%;position:relative}.el-col-sm-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 0%;max-width:0}.el-col-xl-0.is-guttered{display:none}.el-col-xl-offset-0{margin-left:0}.el-col-xl-pull-0{position:relative;right:0}.el-col-xl-push-0{left:0;position:relative}.el-col-xl-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-xl-1,.el-col-xl-1.is-guttered{display:block}.el-col-xl-offset-1{margin-left:4.1666666667%}.el-col-xl-pull-1{position:relative;right:4.1666666667%}.el-col-xl-push-1{left:4.1666666667%;position:relative}.el-col-xl-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-xl-2,.el-col-xl-2.is-guttered{display:block}.el-col-xl-offset-2{margin-left:8.3333333333%}.el-col-xl-pull-2{position:relative;right:8.3333333333%}.el-col-xl-push-2{left:8.3333333333%;position:relative}.el-col-xl-3{flex:0 0 12.5%;max-width:12.5%}.el-col-xl-3,.el-col-xl-3.is-guttered{display:block}.el-col-xl-offset-3{margin-left:12.5%}.el-col-xl-pull-3{position:relative;right:12.5%}.el-col-xl-push-3{left:12.5%;position:relative}.el-col-xl-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-xl-4,.el-col-xl-4.is-guttered{display:block}.el-col-xl-offset-4{margin-left:16.6666666667%}.el-col-xl-pull-4{position:relative;right:16.6666666667%}.el-col-xl-push-4{left:16.6666666667%;position:relative}.el-col-xl-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-xl-5,.el-col-xl-5.is-guttered{display:block}.el-col-xl-offset-5{margin-left:20.8333333333%}.el-col-xl-pull-5{position:relative;right:20.8333333333%}.el-col-xl-push-5{left:20.8333333333%;position:relative}.el-col-xl-6{flex:0 0 25%;max-width:25%}.el-col-xl-6,.el-col-xl-6.is-guttered{display:block}.el-col-xl-offset-6{margin-left:25%}.el-col-xl-pull-6{position:relative;right:25%}.el-col-xl-push-6{left:25%;position:relative}.el-col-xl-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-xl-10.is-guttered{display:block}.el-col-xl-offset-10{margin-left:41.6666666667%}.el-col-xl-pull-10{position:relative;right:41.6666666667%}.el-col-xl-push-10{left:41.6666666667%;position:relative}.el-col-xl-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-xl-11.is-guttered{display:block}.el-col-xl-offset-11{margin-left:45.8333333333%}.el-col-xl-pull-11{position:relative;right:45.8333333333%}.el-col-xl-push-11{left:45.8333333333%;position:relative}.el-col-xl-12{display:block;flex:0 0 50%;max-width:50%}.el-col-xl-12.is-guttered{display:block}.el-col-xl-offset-12{margin-left:50%}.el-col-xl-pull-12{position:relative;right:50%}.el-col-xl-push-12{left:50%;position:relative}.el-col-xl-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px;box-sizing:border-box;height:12px;position:relative;width:280px}.el-color-alpha-slider__bar{background:linear-gradient(to right,hsla(0,0%,100%,0) 0,var(--el-bg-color) 100%);height:100%;position:relative}.el-color-alpha-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-alpha-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-picker.is-focused .el-color-picker__trigger{border-color:var(--el-color-primary)}.el-color-picker.is-disabled .el-color-picker__trigger{cursor:not-allowed}.el-color-picker--large{height:40px}.el-color-picker--large .el-color-picker__trigger{height:40px;width:40px}.el-color-picker--large .el-color-picker__mask{height:38px;width:38px}.el-color-picker--small{height:24px}.el-color-picker--small .el-color-picker__trigger{height:24px;width:24px}.el-color-picker--small .el-color-picker__mask{height:22px;width:22px}.el-color-picker--small .el-color-picker__empty,.el-color-picker--small .el-color-picker__icon{transform:scale(.8)}.el-color-picker__mask{background-color:hsla(0,0%,100%,.7);border-radius:4px;cursor:not-allowed;height:30px;left:1px;position:absolute;top:1px;width:30px;z-index:1}.el-color-picker__trigger{align-items:center;border:1px solid var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker 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5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid 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var(--el-transition-duration)}.dialog-fade-leave-active .el-overlay-dialog{animation:dialog-fade-out var(--el-transition-duration)}@keyframes dialog-fade-in{0%{opacity:0;transform:translate3d(0,-20px,0)}to{opacity:1;transform:translateZ(0)}}@keyframes dialog-fade-out{0%{opacity:1;transform:translateZ(0)}to{opacity:0;transform:translate3d(0,-20px,0)}}@keyframes modal-fade-in{0%{opacity:0}to{opacity:1}}@keyframes modal-fade-out{0%{opacity:1}to{opacity:0}}.el-divider{position:relative}.el-divider--horizontal{border-top:1px var(--el-border-color) var(--el-border-style);display:block;height:1px;margin:24px 0;width:100%}.el-divider--vertical{border-left:1px var(--el-border-color) var(--el-border-style);display:inline-block;height:1em;margin:0 8px;position:relative;vertical-align:middle;width:1px}.el-divider__text{background-color:var(--el-bg-color);color:var(--el-text-color-primary);font-size:14px;font-weight:500;padding:0 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.ttb{transform:translate(0)}.el-drawer__sr-focus:focus{outline:none!important}.el-drawer__header{align-items:center;color:#72767b;display:flex;margin-bottom:32px;padding:var(--el-drawer-padding-primary);padding-bottom:0}.el-drawer__header>:first-child{flex:1}.el-drawer__title{flex:1;font-size:16px;line-height:inherit;margin:0}.el-drawer__footer{padding:var(--el-drawer-padding-primary);padding-top:10px;text-align:right}.el-drawer__close-btn{background-color:transparent;border:none;color:inherit;cursor:pointer;display:inline-flex;font-size:var(--el-font-size-extra-large);outline:none}.el-drawer__close-btn:focus i,.el-drawer__close-btn:hover 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.el-popper__arrow:before{border:1px solid var(--el-border-color-light)}.el-dropdown__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-dropdown__popper .el-dropdown-menu{border:none}.el-dropdown__popper .el-dropdown__popper-selfdefine{outline:none}.el-dropdown__popper .el-scrollbar__bar{z-index:calc(var(--el-dropdown-menu-index) + 1)}.el-dropdown__popper .el-dropdown__list{box-sizing:border-box;list-style:none;margin:0;padding:0}.el-dropdown 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var(--el-transition-duration);width:var(--el-notification-width);z-index:9999}.el-notification.right{right:16px}.el-notification.left{left:16px}.el-notification__group{margin-left:var(--el-notification-group-margin-left);margin-right:var(--el-notification-group-margin-right)}.el-notification__title{color:var(--el-notification-title-color);font-size:var(--el-notification-title-font-size);font-weight:700;line-height:var(--el-notification-icon-size);margin:0}.el-notification__content{color:var(--el-notification-content-color);font-size:var(--el-notification-content-font-size);line-height:24px;margin:6px 0 0}.el-notification__content p{margin:0}.el-notification .el-notification__icon{font-size:var(--el-notification-icon-size);height:var(--el-notification-icon-size);width:var(--el-notification-icon-size)}.el-notification .el-notification__closeBtn{color:var(--el-notification-close-color);cursor:pointer;font-size:var(--el-notification-close-font-size);position:absolute;right:15px;top:18px}.el-notification .el-notification__closeBtn:hover{color:var(--el-notification-close-hover-color)}.el-notification .el-notification--success{--el-notification-icon-color:var(--el-color-success);color:var(--el-notification-icon-color)}.el-notification .el-notification--info{--el-notification-icon-color:var(--el-color-info);color:var(--el-notification-icon-color)}.el-notification .el-notification--warning{--el-notification-icon-color:var(--el-color-warning);color:var(--el-notification-icon-color)}.el-notification .el-notification--error{--el-notification-icon-color:var(--el-color-error);color:var(--el-notification-icon-color)}.el-notification-fade-enter-from.right{right:0;transform:translateX(100%)}.el-notification-fade-enter-from.left{left:0;transform:translateX(-100%)}.el-notification-fade-leave-to{opacity:0}.el-overlay{background-color:var(--el-overlay-color-lighter);bottom:0;height:100%;left:0;overflow:auto;position:fixed;right:0;top:0;z-index:2000}.el-overlay .el-overlay-root{height:0}.el-page-header.is-contentful .el-page-header__main{border-top:1px solid var(--el-border-color-light);margin-top:16px}.el-page-header__header{align-items:center;display:flex;justify-content:space-between;line-height:24px}.el-page-header__left{align-items:center;display:flex;margin-right:40px;position:relative}.el-page-header__back{align-items:center;cursor:pointer;display:flex}.el-page-header__left .el-divider--vertical{margin:0 16px}.el-page-header__icon{align-items:center;display:flex;font-size:16px;margin-right:10px}.el-page-header__icon .el-icon{font-size:inherit}.el-page-header__title{font-size:14px;font-weight:500}.el-page-header__content{color:var(--el-text-color-primary);font-size:18px}.el-page-header__breadcrumb{margin-bottom:16px}.el-pagination{--el-pagination-font-size:14px;--el-pagination-bg-color:var(--el-fill-color-blank);--el-pagination-text-color:var(--el-text-color-primary);--el-pagination-border-radius:2px;--el-pagination-button-color:var(--el-text-color-primary);--el-pagination-button-width:32px;--el-pagination-button-height:32px;--el-pagination-button-disabled-color:var(--el-text-color-placeholder);--el-pagination-button-disabled-bg-color:var(--el-fill-color-blank);--el-pagination-button-bg-color:var(--el-fill-color);--el-pagination-hover-color:var(--el-color-primary);--el-pagination-font-size-small:12px;--el-pagination-button-width-small:24px;--el-pagination-button-height-small:24px;--el-pagination-button-width-large:40px;--el-pagination-button-height-large:40px;--el-pagination-item-gap:16px;align-items:center;color:var(--el-pagination-text-color);display:flex;font-size:var(--el-pagination-font-size);font-weight:400;white-space:nowrap}.el-pagination .el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small 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.el-transfer-panel__header{align-items:center;background:var(--el-transfer-panel-header-bg-color);border:1px solid var(--el-transfer-border-color);border-top-left-radius:var(--el-transfer-border-radius);border-top-right-radius:var(--el-transfer-border-radius);box-sizing:border-box;color:var(--el-color-black);display:flex;height:var(--el-transfer-panel-header-height);margin:0;padding-left:15px}.el-transfer-panel .el-transfer-panel__header .el-checkbox{align-items:center;display:flex;position:relative;width:100%}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label{color:var(--el-text-color-primary);font-size:16px;font-weight:400}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label span{color:var(--el-text-color-secondary);font-size:12px;font-weight:400;position:absolute;right:15px;top:50%;transform:translate3d(0,-50%,0)}.el-transfer-panel .el-transfer-panel__footer{background:var(--el-bg-color-overlay);border:1px solid 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0;overflow:hidden;padding:0;width:var(--el-upload-list-picture-card-size)}.el-upload-list--picture-card .el-upload-list__item .el-icon--check,.el-upload-list--picture-card .el-upload-list__item .el-icon--circle-check{color:#fff}.el-upload-list--picture-card .el-upload-list__item .el-icon--close{display:none}.el-upload-list--picture-card .el-upload-list__item:hover .el-upload-list__item-status-label{display:block;opacity:0}.el-upload-list--picture-card .el-upload-list__item:hover .el-progress__text{display:block}.el-upload-list--picture-card .el-upload-list__item .el-upload-list__item-name{display:none}.el-upload-list--picture-card .el-upload-list__item-thumbnail{height:100%;-o-object-fit:contain;object-fit:contain;width:100%}.el-upload-list--picture-card .el-upload-list__item-status-label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-list--picture-card .el-upload-list__item-status-label 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img{display:block;height:100%;width:100%}.el-upload-cover__label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-cover__label i{color:#fff;font-size:12px;margin-top:11px;transform:rotate(-45deg)}.el-upload-cover__progress{display:inline-block;position:static;vertical-align:middle;width:243px}.el-upload-cover__progress+.el-upload__inner{opacity:0}.el-upload-cover__content{height:100%;left:0;position:absolute;top:0;width:100%}.el-upload-cover__interact{background-color:var(--el-overlay-color-light);bottom:0;height:100%;left:0;position:absolute;text-align:center;width:100%}.el-upload-cover__interact .btn{color:#fff;cursor:pointer;display:inline-block;font-size:14px;margin-top:60px;transition:var(--el-transition-md-fade);vertical-align:middle}.el-upload-cover__interact .btn i{margin-top:0}.el-upload-cover__interact .btn span{opacity:0;transition:opacity .15s linear}.el-upload-cover__interact 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var(--el-border-color-light)}.el-popper.is-light>.el-popper__arrow:before{right:0}.el-popper.is-pure{padding:0}.el-popper__arrow,.el-popper__arrow:before{height:10px;position:absolute;width:10px;z-index:-1}.el-popper__arrow:before{background:var(--el-text-color-primary);box-sizing:border-box;content:" 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tabindex="-1"><!--[--><!--[-->Parts<!--]--><!--[--><!--]--><!--]--></li><!--]--></ul></div><!--]--><!--v-if--><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2009;position:absolute;left:0;top:0;display:none;" class="el-popper is-pure is-light" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><div class="el-menu--vertical el-menu--popup-container"><ul class="el-menu el-menu--popup el-menu--popup-right-start" style="--el-menu-level:1;"><!--[--><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Hardware<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Model<!--]--><!--[--><!--]--><!--]--></li><!--]--></ul></div><!--]--><!--v-if--><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2010;position:absolute;left:0;top:0;display:none;" class="el-popper is-pure is-light" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><div class="el-menu--vertical el-menu--popup-container"><ul class="el-menu el-menu--popup el-menu--popup-right-start" style="--el-menu-level:1;"><!--[--><li class="el-menu-item is-active" role="menuitem" tabindex="-1"><!--[--><!--[-->Members<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Attributions<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Contribution<!--]--><!--[--><!--]--><!--]--></li><!--]--></ul></div><!--]--><!--v-if--><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2011;position:absolute;left:0;top:0;display:none;" class="el-popper is-pure is-light" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><div class="el-menu--vertical el-menu--popup-container"><ul class="el-menu el-menu--popup el-menu--popup-right-start" style="--el-menu-level:1;"><!--[--><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Education<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Integrated HP<!--]--><!--[--><!--]--><!--]--></li><!--]--></ul></div><!--]--><!--v-if--><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2012;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Professor <br> Doctoral Supervisor</div><div class="mt-2">The research field belongs to biomedical testing.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2013;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Professor <br> Doctoral Supervisor</div><div class="mt-2">The research field belongs to the cross field of neurobiology, space biology and biological analysis technology.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2014;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Professor <br> Doctoral Supervisor</div><div class="mt-2">The research field belongs to space life science payloads, in vitro diagnostic technology and equipment for clinical medicine</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2015;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Advisor <br> Postgraduate</div><div class="mt-2">Participated in iGEM for five years, who is our team’s eldest brother -- brightest lighthouse on our voyage in 2024.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2016;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Advisor <br> Postgraduate</div><div class="mt-2">2017 Captain of BIT majoring in biomedical engineering, who is our guardian angel on our expedition to synthetic biology.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2017;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Advisor <br> Postgraduate</div><div class="mt-2">A girl who is passionate about synthetic biology.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2018;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Advisor <br> Postgraduate</div><div class="mt-2">(Natural Language Processing, Medical Image Processing, Microfluidic Chip)：hope you will turn what you learn into your own skill.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2019;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Advisor <br> Postgraduate</div><div class="mt-2">Captain of 2023 BIT, believe fortune favors the bold!</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2020;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Senior <br> Biomedical Engineering</div><div class="mt-2">Eat better, live better.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2021;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biotechnology - Biological Sciences</div><div class="mt-2">This year, I led the strain construction, verification, and characterization of biological circuits in wet lab experiments. Our project aims to advance biotoxicology detection methods.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2022;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Senior <br> Biomedical Engineering</div><div class="mt-2">A doer with full of enthusiasm. A dreamer with full of passion.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2023;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Senior <br> Biomedical Engineering</div><div class="mt-2">Always appreciate the past, cherish the present and be enthusiastic to the future.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2024;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biotechnology - AI</div><div class="mt-2">A spirited young girl, driven by perfection, infuses everyday life with idealism, turning reality’s challenges into a realm of possibilities. Her pursuit of purpose is a continuous journey of discovery.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2025;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biomedical Engineering</div><div class="mt-2">As a member of the biology group, I have learned a lot of skills and further explored the field of synthetic biology that I am very interested in. I hope we can have a good result.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2026;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Senior <br> Software Engineering</div><div class="mt-2">Dive deep into the neuroscience and healthcare. TECH FOR GOOD!</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2027;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Senior <br> Software Engineering</div><div class="mt-2">A student who likes code, likes to explore and likes the unknown world.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2028;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Sophomore <br> Optoelectronic Information and Intelligent Sensing</div><div class="mt-2">An idealistic girl who wants to become a promoter of the intersection of medicine and engineering.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2029;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Sophomore <br> Product Design</div><div class="mt-2">A life enthusiast, a sports aficionado, and a visionary who seeks to elevate the world through the transformative power of art and design.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2030;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biomedical Engineering</div><div class="mt-2">Hello everyone, my name is Li Tian. Nice to meet you.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2031;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biology</div><div class="mt-2">He’s driven to use advanced life science tech for global benefits, believing it can solve key issues like disease and environmental damage, creating a healthier, sustainable world.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2032;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Twelfth Grade <br> Beijing 101 High School</div><div class="mt-2">Biology is one of the few subjects I am passionate about and willing to devote into a great deal of thought and time.  I chose to participate as team member to deepen my understanding of biology.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2033;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biomedical Engineering</div><div class="mt-2">A junior undergraduate student who loves biology and computers.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2034;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biomedical Engineering</div><div class="mt-2">A boy with an enthusiasm for life, wanna play a role in improving public health.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2035;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Automation</div><div class="mt-2">I am a very enthusiastic online person who is relatively distant offline. If you want to know me, please don&#39;t give up because of offline indifference</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2036;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biomedical Engineering</div><div class="mt-2">Gains Dont Stop!</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2037;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Twelfth Grade <br> Affiliated High School of Renmin University</div><div class="mt-2">I have an upbeat personality with a love for literature, music, and sports. In my free time, I compose music and excel in various ball games. Robotics, however, is my true passion, and I’ve won awards in several competitions.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2038;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Mechanical Engineering</div><div class="mt-2">As a creative hardware designer, I aim to bring our innovative biological ideas to life through practical hardware solutions. I’m thrilled to work with my team to turn our visions into reality.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2039;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Sophomore <br> Biotechnology - Biological Sciences</div><div class="mt-2">A striver and lover of beautiful things and the future, hoping to create their own value!</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2040;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biomedical Engineering</div><div class="mt-2">A sunny and cheerful boy, adept at discovering new ideas and bringing them to life, believes that learning never ends, and looks forward to progressing together with everyone.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2041;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Statistics</div><div class="mt-2">A boy with a passionate for creativity, relishing in-depth exploration and innovative insights. My academic journey has honed my critical thinking and problem-solving skills, readying me for real-world challenges.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--></div><div id="__nuxt"><!--[--><div class="nuxt-loading-indicator" style="position:fixed;top:0;right:0;left:0;pointer-events:none;width:auto;height:3px;opacity:0;background:repeating-linear-gradient(to right,#00dc82 0%,#34cdfe 50%,#0047e1 100%);background-size:Infinity% auto;transform:scaleX(0%);transform-origin:left;transition:transform 0.1s, height 0.4s, opacity 0.4s;z-index:999999;"></div><div class="mx-10"><header class="fixed w-full"><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-desktop px-10 hidden lg:flex"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, 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class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Human Practices<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li></ul><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-mobile px-10 flex lg:hidden"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 2x" class="cursor-pointer py-2" src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div 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w-50 lg:w-80 xl:w-[30rem]" src="https://static.igem.wiki/teams/5358/imgs/banner-member.svg?width=10/height=10/quality=50/blur=3"><div class="grid grid-cols-2 gap-10 md:gap-20 md:grid-cols-3 justify-items-center align-top"><!--[--><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/pi-lvxuefei.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Lv Xuefei<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Primary PI</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/pi-dengyulin.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Deng Yulin<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:5px;margin-top:-3px;">Secondary PI</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/pi-lixiaoqiong.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Li Xiaoqiong<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:-3px;">Secondary PI</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><!--]--></div></div><div class="mt-10 grid grid-cols-2 gap-10 md:grid-cols-3 justify-items-center mx-[2vw]"><!--[--><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/advisor-jianghao.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Jiang Hao<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Advisor</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/advisor-lianyi.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Li Anyi<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-20px;margin-top:;">Advisor</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/advisor-qiancheng.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Qian Cheng<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:5px;margin-top:;">Advisor</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/advisor-wangkun.png?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Wang Kun<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Advisor</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/advisor-yanzihan.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Yan Zihan<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Advisor</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><!--]--></div><div class="m-4 sm:m-16 rounded-xl shadow-lg"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/team1/group.jpg?quality=100/fit=fill 1x, https://static.igem.wiki/teams/5358/team1/group.jpg?quality=100/fit=fill 2x" class="rounded-xl" src="https://static.igem.wiki/teams/5358/team1/group.jpg?quality=100/fit=fill"></div><div class="grid grid-cols-2 gap-10 md:grid-cols-3 justify-items-center mx-[2vw]"><!--[--><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/leader-fangbaoqi.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Fang Baoqi<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Leader</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/leader-fanshuai.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Fan Shuai<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-10px;margin-top:-3px;">Leader</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/leader-liguanlong.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Li Guanlong<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:5px;margin-top:-3px;">Leader</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/leader-wangjianing.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Wang Jianing<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:;">Leader</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-caitong.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Cai Tong<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-10px;margin-top:-2px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-cuiyuan.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Cui Yuan<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-gaopeng.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Gao Peng<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-huoyongyu.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Huo Yongyu<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-jinchuan.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Jin Chuan<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-lisixuan.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Li Sixuan<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-litian.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Li Tian<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-20px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-lixichang.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Li Xichang<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-leezhuohai.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Li Zhuohan<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-pengxingyu.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Peng Xingyu<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-sunjingbang.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Sun Jingbang<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-wangqiheng.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Wang Qiheng<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-wenjunjie.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Wen Junjie<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-yaoyixuan.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Yao Yixuan<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-zhanglingyue.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge 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149.376a29.12 29.12 0 0 0-41.728 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li></ul><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2007;position:absolute;left:0;top:0;display:none;" class="el-popper is-pure is-light" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><div class="el-menu--vertical el-menu--popup-container"><ul class="el-menu el-menu--popup el-menu--popup-right-start" style="--el-menu-level:1;"><!--[--><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Description<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Engineering<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Safety<!--]--><!--[--><!--]--><!--]--></li><!--]--></ul></div><!--]--><!--v-if--><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2008;position:absolute;left:0;top:0;display:none;" class="el-popper is-pure is-light" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><div class="el-menu--vertical el-menu--popup-container"><ul class="el-menu el-menu--popup el-menu--popup-right-start" style="--el-menu-level:1;"><!--[--><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Experiments<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Results<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Notebook<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Parts<!--]--><!--[--><!--]--><!--]--></li><!--]--></ul></div><!--]--><!--v-if--><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2009;position:absolute;left:0;top:0;display:none;" class="el-popper is-pure is-light" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><div class="el-menu--vertical el-menu--popup-container"><ul class="el-menu el-menu--popup el-menu--popup-right-start" style="--el-menu-level:1;"><!--[--><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Hardware<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Model<!--]--><!--[--><!--]--><!--]--></li><!--]--></ul></div><!--]--><!--v-if--><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2010;position:absolute;left:0;top:0;display:none;" class="el-popper is-pure is-light" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><div class="el-menu--vertical el-menu--popup-container"><ul class="el-menu el-menu--popup el-menu--popup-right-start" style="--el-menu-level:1;"><!--[--><li class="el-menu-item is-active" role="menuitem" tabindex="-1"><!--[--><!--[-->Members<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Attributions<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Contribution<!--]--><!--[--><!--]--><!--]--></li><!--]--></ul></div><!--]--><!--v-if--><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2011;position:absolute;left:0;top:0;display:none;" class="el-popper is-pure is-light" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><div class="el-menu--vertical el-menu--popup-container"><ul class="el-menu el-menu--popup el-menu--popup-right-start" style="--el-menu-level:1;"><!--[--><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Education<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Integrated HP<!--]--><!--[--><!--]--><!--]--></li><!--]--></ul></div><!--]--><!--v-if--><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2012;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Professor <br> Doctoral Supervisor</div><div class="mt-2">The research field belongs to biomedical testing.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2013;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Professor <br> Doctoral Supervisor</div><div class="mt-2">The research field belongs to the cross field of neurobiology, space biology and biological analysis technology.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2014;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Professor <br> Doctoral Supervisor</div><div class="mt-2">The research field belongs to space life science payloads, in vitro diagnostic technology and equipment for clinical medicine</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2015;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Advisor <br> Postgraduate</div><div class="mt-2">Participated in iGEM for five years, who is our team’s eldest brother -- brightest lighthouse on our voyage in 2024.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2016;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Advisor <br> Postgraduate</div><div class="mt-2">2017 Captain of BIT majoring in biomedical engineering, who is our guardian angel on our expedition to synthetic biology.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2017;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Advisor <br> Postgraduate</div><div class="mt-2">A girl who is passionate about synthetic biology.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2018;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Advisor <br> Postgraduate</div><div class="mt-2">(Natural Language Processing, Medical Image Processing, Microfluidic Chip)：hope you will turn what you learn into your own skill.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2019;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Advisor <br> Postgraduate</div><div class="mt-2">Captain of 2023 BIT, believe fortune favors the bold!</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2020;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Senior <br> Biomedical Engineering</div><div class="mt-2">Eat better, live better.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2021;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biotechnology - Biological Sciences</div><div class="mt-2">This year, I led the strain construction, verification, and characterization of biological circuits in wet lab experiments. Our project aims to advance biotoxicology detection methods.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2022;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Senior <br> Biomedical Engineering</div><div class="mt-2">A doer with full of enthusiasm. A dreamer with full of passion.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2023;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Senior <br> Biomedical Engineering</div><div class="mt-2">Always appreciate the past, cherish the present and be enthusiastic to the future.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2024;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biotechnology - AI</div><div class="mt-2">A spirited young girl, driven by perfection, infuses everyday life with idealism, turning reality’s challenges into a realm of possibilities. Her pursuit of purpose is a continuous journey of discovery.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2025;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biomedical Engineering</div><div class="mt-2">As a member of the biology group, I have learned a lot of skills and further explored the field of synthetic biology that I am very interested in. I hope we can have a good result.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2026;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Senior <br> Software Engineering</div><div class="mt-2">Dive deep into the neuroscience and healthcare. TECH FOR GOOD!</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2027;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Senior <br> Software Engineering</div><div class="mt-2">A student who likes code, likes to explore and likes the unknown world.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2028;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Sophomore <br> Optoelectronic Information and Intelligent Sensing</div><div class="mt-2">An idealistic girl who wants to become a promoter of the intersection of medicine and engineering.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2029;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Sophomore <br> Product Design</div><div class="mt-2">A life enthusiast, a sports aficionado, and a visionary who seeks to elevate the world through the transformative power of art and design.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2030;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biomedical Engineering</div><div class="mt-2">Hello everyone, my name is Li Tian. Nice to meet you.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2031;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biology</div><div class="mt-2">He’s driven to use advanced life science tech for global benefits, believing it can solve key issues like disease and environmental damage, creating a healthier, sustainable world.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2032;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Twelfth Grade <br> Beijing 101 High School</div><div class="mt-2">Biology is one of the few subjects I am passionate about and willing to devote into a great deal of thought and time.  I chose to participate as team member to deepen my understanding of biology.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2033;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biomedical Engineering</div><div class="mt-2">A junior undergraduate student who loves biology and computers.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2034;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biomedical Engineering</div><div class="mt-2">A boy with an enthusiasm for life, wanna play a role in improving public health.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2035;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Automation</div><div class="mt-2">I am a very enthusiastic online person who is relatively distant offline. If you want to know me, please don&#39;t give up because of offline indifference</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2036;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biomedical Engineering</div><div class="mt-2">Gains Dont Stop!</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2037;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Twelfth Grade <br> Affiliated High School of Renmin University</div><div class="mt-2">I have an upbeat personality with a love for literature, music, and sports. In my free time, I compose music and excel in various ball games. Robotics, however, is my true passion, and I’ve won awards in several competitions.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2038;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Mechanical Engineering</div><div class="mt-2">As a creative hardware designer, I aim to bring our innovative biological ideas to life through practical hardware solutions. I’m thrilled to work with my team to turn our visions into reality.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2039;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Sophomore <br> Biotechnology - Biological Sciences</div><div class="mt-2">A striver and lover of beautiful things and the future, hoping to create their own value!</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2040;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Biomedical Engineering</div><div class="mt-2">A sunny and cheerful boy, adept at discovering new ideas and bringing them to life, believes that learning never ends, and looks forward to progressing together with everyone.</div><!--]--><!--]--><span class="el-popper__arrow" style="position:absolute;" data-popper-arrow></span><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2041;position:absolute;left:0;top:0;width:200px;display:none;" class="el-popper is-dark el-popover" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><!--v-if--><!--[--><div class="text-lg font-bold">Junior <br> Statistics</div><div class="mt-2">A boy with a passionate for creativity, relishing in-depth exploration and innovative insights. 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el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/pi-dengyulin.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Deng Yulin<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:5px;margin-top:-3px;">Secondary PI</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/pi-lixiaoqiong.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Li Xiaoqiong<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:-3px;">Secondary PI</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><!--]--></div></div><div class="mt-10 grid grid-cols-2 gap-10 md:grid-cols-3 justify-items-center mx-[2vw]"><!--[--><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/advisor-jianghao.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Jiang Hao<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Advisor</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/advisor-lianyi.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Li Anyi<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-20px;margin-top:;">Advisor</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/advisor-qiancheng.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Qian Cheng<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:5px;margin-top:;">Advisor</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/advisor-wangkun.png?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Wang Kun<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Advisor</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/advisor-yanzihan.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Yan Zihan<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Advisor</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><!--]--></div><div class="m-4 sm:m-16 rounded-xl shadow-lg"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/team1/group.jpg?quality=100/fit=fill 1x, https://static.igem.wiki/teams/5358/team1/group.jpg?quality=100/fit=fill 2x" class="rounded-xl" src="https://static.igem.wiki/teams/5358/team1/group.jpg?quality=100/fit=fill"></div><div class="grid grid-cols-2 gap-10 md:grid-cols-3 justify-items-center mx-[2vw]"><!--[--><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/leader-fangbaoqi.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Fang Baoqi<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Leader</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/leader-fanshuai.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Fan Shuai<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-10px;margin-top:-3px;">Leader</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/leader-liguanlong.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Li Guanlong<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:5px;margin-top:-3px;">Leader</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/leader-wangjianing.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Wang Jianing<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:;">Leader</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-caitong.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Cai Tong<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-10px;margin-top:-2px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-cuiyuan.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Cui Yuan<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-gaopeng.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Gao Peng<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-huoyongyu.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Huo Yongyu<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-jinchuan.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Jin Chuan<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-lisixuan.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Li Sixuan<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img 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item"><!--[-->Li Xichang<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-leezhuohai.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Li Zhuohan<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-pengxingyu.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Peng Xingyu<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-sunjingbang.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Sun Jingbang<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-wangqiheng.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Wang Qiheng<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-wenjunjie.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Wen Junjie<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-yaoyixuan.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Yao Yixuan<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:-2px;margin-top:-3px;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-zhanglingyue.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Zhang Lingyue<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-zhouxinbo.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Zhou Xinbo<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" src="https://static.igem.wiki/teams/5358/team1/students-zhuxiaohang.jpg?width=50/height=50/quality=50/fit=fill/blur=3"><!--]--></div><div class="el-card__footer"><!--[--><div class="el-badge item"><!--[-->Zhu Xiaohang<!--]--><sup class="el-badge__content el-badge__content--danger is-fixed" style="margin-right:10px;margin-top:;">Student</sup></div><!--]--></div></div><!--teleport start--><!--teleport end--><!--]--></div><div class="m-2 inline-block m-2"><!--[--><div class="el-card is-hover-shadow el-tooltip__trigger"><!--v-if--><div class="el-card__body" style=""><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" loading="lazy" data-nuxt-img class="aspect-[3/4] w-full" 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text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a aria-current="page" href="/bit/member" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/member/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":11,"prerenderedAt":12},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{},"/member",1727871092384]</script>
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diff --git a/.output/public/model/_payload.json b/.output/public/model/_payload.json
index 68b29bf16884beb672016fe5c09d004df13dfed6..470d9e0921455f9d4f08a49629dc17434689f43e 100644
--- a/.output/public/model/_payload.json
+++ b/.output/public/model/_payload.json
@@ -1 +1 @@
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\ No newline at end of file
+[{"data":1,"prerenderedAt":3},["Reactive",2],{},1727871092372]
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diff --git a/.output/public/model/index.html b/.output/public/model/index.html
index 98de59914bdaa5923be2179bbaa2cbbe2dd9c9fe..2b9996b2d15ee7c4576f2afb49f20527385f494b 100644
--- a/.output/public/model/index.html
+++ b/.output/public/model/index.html
@@ -5,20 +5,20 @@
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.el-button--info:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button{--el-button-font-weight:var(--el-font-weight-primary);--el-button-border-color:var(--el-border-color);--el-button-bg-color:var(--el-fill-color-blank);--el-button-text-color:var(--el-text-color-regular);--el-button-disabled-text-color:var(--el-disabled-text-color);--el-button-disabled-bg-color:var(--el-fill-color-blank);--el-button-disabled-border-color:var(--el-border-color-light);--el-button-divide-border-color:hsla(0,0%,100%,.5);--el-button-hover-text-color:var(--el-color-primary);--el-button-hover-bg-color:var(--el-color-primary-light-9);--el-button-hover-border-color:var(--el-color-primary-light-7);--el-button-active-text-color:var(--el-button-hover-text-color);--el-button-active-border-color:var(--el-color-primary);--el-button-active-bg-color:var(--el-button-hover-bg-color);--el-button-outline-color:var(--el-color-primary-light-5);--el-button-hover-link-text-color:var(--el-color-info);--el-button-active-color:var(--el-text-color-primary);align-items:center;-webkit-appearance:none;background-color:var(--el-button-bg-color);border:var(--el-border);border-color:var(--el-button-border-color);box-sizing:border-box;color:var(--el-button-text-color);cursor:pointer;display:inline-flex;font-weight:var(--el-button-font-weight);height:32px;justify-content:center;line-height:1;outline:none;text-align:center;transition:.1s;-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-button:hover{background-color:var(--el-button-hover-bg-color);border-color:var(--el-button-hover-border-color);color:var(--el-button-hover-text-color);outline:none}.el-button:active{background-color:var(--el-button-active-bg-color);border-color:var(--el-button-active-border-color);color:var(--el-button-active-text-color);outline:none}.el-button:focus-visible{outline:2px 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1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper 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.el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags 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.el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large 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11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) 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cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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79.1666666667%;max-width:79.1666666667%}.el-col-xs-19.is-guttered{display:block}.el-col-xs-offset-19{margin-left:79.1666666667%}.el-col-xs-pull-19{position:relative;right:79.1666666667%}.el-col-xs-push-19{left:79.1666666667%;position:relative}.el-col-xs-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xs-20.is-guttered{display:block}.el-col-xs-offset-20{margin-left:83.3333333333%}.el-col-xs-pull-20{position:relative;right:83.3333333333%}.el-col-xs-push-20{left:83.3333333333%;position:relative}.el-col-xs-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xs-21.is-guttered{display:block}.el-col-xs-offset-21{margin-left:87.5%}.el-col-xs-pull-21{position:relative;right:87.5%}.el-col-xs-push-21{left:87.5%;position:relative}.el-col-xs-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 0%;max-width:0}.el-col-sm-0.is-guttered{display:none}.el-col-sm-offset-0{margin-left:0}.el-col-sm-pull-0{position:relative;right:0}.el-col-sm-push-0{left:0;position:relative}.el-col-sm-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-sm-1,.el-col-sm-1.is-guttered{display:block}.el-col-sm-offset-1{margin-left:4.1666666667%}.el-col-sm-pull-1{position:relative;right:4.1666666667%}.el-col-sm-push-1{left:4.1666666667%;position:relative}.el-col-sm-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-sm-2,.el-col-sm-2.is-guttered{display:block}.el-col-sm-offset-2{margin-left:8.3333333333%}.el-col-sm-pull-2{position:relative;right:8.3333333333%}.el-col-sm-push-2{left:8.3333333333%;position:relative}.el-col-sm-3{flex:0 0 12.5%;max-width:12.5%}.el-col-sm-3,.el-col-sm-3.is-guttered{display:block}.el-col-sm-offset-3{margin-left:12.5%}.el-col-sm-pull-3{position:relative;right:12.5%}.el-col-sm-push-3{left:12.5%;position:relative}.el-col-sm-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-sm-4,.el-col-sm-4.is-guttered{display:block}.el-col-sm-offset-4{margin-left:16.6666666667%}.el-col-sm-pull-4{position:relative;right:16.6666666667%}.el-col-sm-push-4{left:16.6666666667%;position:relative}.el-col-sm-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-sm-5,.el-col-sm-5.is-guttered{display:block}.el-col-sm-offset-5{margin-left:20.8333333333%}.el-col-sm-pull-5{position:relative;right:20.8333333333%}.el-col-sm-push-5{left:20.8333333333%;position:relative}.el-col-sm-6{flex:0 0 25%;max-width:25%}.el-col-sm-6,.el-col-sm-6.is-guttered{display:block}.el-col-sm-offset-6{margin-left:25%}.el-col-sm-pull-6{position:relative;right:25%}.el-col-sm-push-6{left:25%;position:relative}.el-col-sm-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 0%;max-width:0}.el-col-xl-0.is-guttered{display:none}.el-col-xl-offset-0{margin-left:0}.el-col-xl-pull-0{position:relative;right:0}.el-col-xl-push-0{left:0;position:relative}.el-col-xl-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-xl-1,.el-col-xl-1.is-guttered{display:block}.el-col-xl-offset-1{margin-left:4.1666666667%}.el-col-xl-pull-1{position:relative;right:4.1666666667%}.el-col-xl-push-1{left:4.1666666667%;position:relative}.el-col-xl-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-xl-2,.el-col-xl-2.is-guttered{display:block}.el-col-xl-offset-2{margin-left:8.3333333333%}.el-col-xl-pull-2{position:relative;right:8.3333333333%}.el-col-xl-push-2{left:8.3333333333%;position:relative}.el-col-xl-3{flex:0 0 12.5%;max-width:12.5%}.el-col-xl-3,.el-col-xl-3.is-guttered{display:block}.el-col-xl-offset-3{margin-left:12.5%}.el-col-xl-pull-3{position:relative;right:12.5%}.el-col-xl-push-3{left:12.5%;position:relative}.el-col-xl-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-xl-4,.el-col-xl-4.is-guttered{display:block}.el-col-xl-offset-4{margin-left:16.6666666667%}.el-col-xl-pull-4{position:relative;right:16.6666666667%}.el-col-xl-push-4{left:16.6666666667%;position:relative}.el-col-xl-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-xl-5,.el-col-xl-5.is-guttered{display:block}.el-col-xl-offset-5{margin-left:20.8333333333%}.el-col-xl-pull-5{position:relative;right:20.8333333333%}.el-col-xl-push-5{left:20.8333333333%;position:relative}.el-col-xl-6{flex:0 0 25%;max-width:25%}.el-col-xl-6,.el-col-xl-6.is-guttered{display:block}.el-col-xl-offset-6{margin-left:25%}.el-col-xl-pull-6{position:relative;right:25%}.el-col-xl-push-6{left:25%;position:relative}.el-col-xl-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-xl-10.is-guttered{display:block}.el-col-xl-offset-10{margin-left:41.6666666667%}.el-col-xl-pull-10{position:relative;right:41.6666666667%}.el-col-xl-push-10{left:41.6666666667%;position:relative}.el-col-xl-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-xl-11.is-guttered{display:block}.el-col-xl-offset-11{margin-left:45.8333333333%}.el-col-xl-pull-11{position:relative;right:45.8333333333%}.el-col-xl-push-11{left:45.8333333333%;position:relative}.el-col-xl-12{display:block;flex:0 0 50%;max-width:50%}.el-col-xl-12.is-guttered{display:block}.el-col-xl-offset-12{margin-left:50%}.el-col-xl-pull-12{position:relative;right:50%}.el-col-xl-push-12{left:50%;position:relative}.el-col-xl-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px;box-sizing:border-box;height:12px;position:relative;width:280px}.el-color-alpha-slider__bar{background:linear-gradient(to right,hsla(0,0%,100%,0) 0,var(--el-bg-color) 100%);height:100%;position:relative}.el-color-alpha-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-alpha-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-picker.is-focused .el-color-picker__trigger{border-color:var(--el-color-primary)}.el-color-picker.is-disabled .el-color-picker__trigger{cursor:not-allowed}.el-color-picker--large{height:40px}.el-color-picker--large .el-color-picker__trigger{height:40px;width:40px}.el-color-picker--large .el-color-picker__mask{height:38px;width:38px}.el-color-picker--small{height:24px}.el-color-picker--small .el-color-picker__trigger{height:24px;width:24px}.el-color-picker--small .el-color-picker__mask{height:22px;width:22px}.el-color-picker--small .el-color-picker__empty,.el-color-picker--small .el-color-picker__icon{transform:scale(.8)}.el-color-picker__mask{background-color:hsla(0,0%,100%,.7);border-radius:4px;cursor:not-allowed;height:30px;left:1px;position:absolute;top:1px;width:30px;z-index:1}.el-color-picker__trigger{align-items:center;border:1px solid var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker 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5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid 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var(--el-transition-duration)}.dialog-fade-leave-active .el-overlay-dialog{animation:dialog-fade-out var(--el-transition-duration)}@keyframes dialog-fade-in{0%{opacity:0;transform:translate3d(0,-20px,0)}to{opacity:1;transform:translateZ(0)}}@keyframes dialog-fade-out{0%{opacity:1;transform:translateZ(0)}to{opacity:0;transform:translate3d(0,-20px,0)}}@keyframes modal-fade-in{0%{opacity:0}to{opacity:1}}@keyframes modal-fade-out{0%{opacity:1}to{opacity:0}}.el-divider{position:relative}.el-divider--horizontal{border-top:1px var(--el-border-color) var(--el-border-style);display:block;height:1px;margin:24px 0;width:100%}.el-divider--vertical{border-left:1px var(--el-border-color) var(--el-border-style);display:inline-block;height:1em;margin:0 8px;position:relative;vertical-align:middle;width:1px}.el-divider__text{background-color:var(--el-bg-color);color:var(--el-text-color-primary);font-size:14px;font-weight:500;padding:0 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.ttb{transform:translate(0)}.el-drawer__sr-focus:focus{outline:none!important}.el-drawer__header{align-items:center;color:#72767b;display:flex;margin-bottom:32px;padding:var(--el-drawer-padding-primary);padding-bottom:0}.el-drawer__header>:first-child{flex:1}.el-drawer__title{flex:1;font-size:16px;line-height:inherit;margin:0}.el-drawer__footer{padding:var(--el-drawer-padding-primary);padding-top:10px;text-align:right}.el-drawer__close-btn{background-color:transparent;border:none;color:inherit;cursor:pointer;display:inline-flex;font-size:var(--el-font-size-extra-large);outline:none}.el-drawer__close-btn:focus i,.el-drawer__close-btn:hover 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.el-popper__arrow:before{border:1px solid var(--el-border-color-light)}.el-dropdown__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-dropdown__popper .el-dropdown-menu{border:none}.el-dropdown__popper .el-dropdown__popper-selfdefine{outline:none}.el-dropdown__popper .el-scrollbar__bar{z-index:calc(var(--el-dropdown-menu-index) + 1)}.el-dropdown__popper .el-dropdown__list{box-sizing:border-box;list-style:none;margin:0;padding:0}.el-dropdown 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var(--el-transition-duration);width:var(--el-notification-width);z-index:9999}.el-notification.right{right:16px}.el-notification.left{left:16px}.el-notification__group{margin-left:var(--el-notification-group-margin-left);margin-right:var(--el-notification-group-margin-right)}.el-notification__title{color:var(--el-notification-title-color);font-size:var(--el-notification-title-font-size);font-weight:700;line-height:var(--el-notification-icon-size);margin:0}.el-notification__content{color:var(--el-notification-content-color);font-size:var(--el-notification-content-font-size);line-height:24px;margin:6px 0 0}.el-notification__content p{margin:0}.el-notification .el-notification__icon{font-size:var(--el-notification-icon-size);height:var(--el-notification-icon-size);width:var(--el-notification-icon-size)}.el-notification .el-notification__closeBtn{color:var(--el-notification-close-color);cursor:pointer;font-size:var(--el-notification-close-font-size);position:absolute;right:15px;top:18px}.el-notification .el-notification__closeBtn:hover{color:var(--el-notification-close-hover-color)}.el-notification .el-notification--success{--el-notification-icon-color:var(--el-color-success);color:var(--el-notification-icon-color)}.el-notification .el-notification--info{--el-notification-icon-color:var(--el-color-info);color:var(--el-notification-icon-color)}.el-notification .el-notification--warning{--el-notification-icon-color:var(--el-color-warning);color:var(--el-notification-icon-color)}.el-notification .el-notification--error{--el-notification-icon-color:var(--el-color-error);color:var(--el-notification-icon-color)}.el-notification-fade-enter-from.right{right:0;transform:translateX(100%)}.el-notification-fade-enter-from.left{left:0;transform:translateX(-100%)}.el-notification-fade-leave-to{opacity:0}.el-overlay{background-color:var(--el-overlay-color-lighter);bottom:0;height:100%;left:0;overflow:auto;position:fixed;right:0;top:0;z-index:2000}.el-overlay .el-overlay-root{height:0}.el-page-header.is-contentful .el-page-header__main{border-top:1px solid var(--el-border-color-light);margin-top:16px}.el-page-header__header{align-items:center;display:flex;justify-content:space-between;line-height:24px}.el-page-header__left{align-items:center;display:flex;margin-right:40px;position:relative}.el-page-header__back{align-items:center;cursor:pointer;display:flex}.el-page-header__left .el-divider--vertical{margin:0 16px}.el-page-header__icon{align-items:center;display:flex;font-size:16px;margin-right:10px}.el-page-header__icon .el-icon{font-size:inherit}.el-page-header__title{font-size:14px;font-weight:500}.el-page-header__content{color:var(--el-text-color-primary);font-size:18px}.el-page-header__breadcrumb{margin-bottom:16px}.el-pagination{--el-pagination-font-size:14px;--el-pagination-bg-color:var(--el-fill-color-blank);--el-pagination-text-color:var(--el-text-color-primary);--el-pagination-border-radius:2px;--el-pagination-button-color:var(--el-text-color-primary);--el-pagination-button-width:32px;--el-pagination-button-height:32px;--el-pagination-button-disabled-color:var(--el-text-color-placeholder);--el-pagination-button-disabled-bg-color:var(--el-fill-color-blank);--el-pagination-button-bg-color:var(--el-fill-color);--el-pagination-hover-color:var(--el-color-primary);--el-pagination-font-size-small:12px;--el-pagination-button-width-small:24px;--el-pagination-button-height-small:24px;--el-pagination-button-width-large:40px;--el-pagination-button-height-large:40px;--el-pagination-item-gap:16px;align-items:center;color:var(--el-pagination-text-color);display:flex;font-size:var(--el-pagination-font-size);font-weight:400;white-space:nowrap}.el-pagination .el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small 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.el-transfer-panel__header{align-items:center;background:var(--el-transfer-panel-header-bg-color);border:1px solid var(--el-transfer-border-color);border-top-left-radius:var(--el-transfer-border-radius);border-top-right-radius:var(--el-transfer-border-radius);box-sizing:border-box;color:var(--el-color-black);display:flex;height:var(--el-transfer-panel-header-height);margin:0;padding-left:15px}.el-transfer-panel .el-transfer-panel__header .el-checkbox{align-items:center;display:flex;position:relative;width:100%}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label{color:var(--el-text-color-primary);font-size:16px;font-weight:400}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label span{color:var(--el-text-color-secondary);font-size:12px;font-weight:400;position:absolute;right:15px;top:50%;transform:translate3d(0,-50%,0)}.el-transfer-panel .el-transfer-panel__footer{background:var(--el-bg-color-overlay);border:1px solid 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0;overflow:hidden;padding:0;width:var(--el-upload-list-picture-card-size)}.el-upload-list--picture-card .el-upload-list__item .el-icon--check,.el-upload-list--picture-card .el-upload-list__item .el-icon--circle-check{color:#fff}.el-upload-list--picture-card .el-upload-list__item .el-icon--close{display:none}.el-upload-list--picture-card .el-upload-list__item:hover .el-upload-list__item-status-label{display:block;opacity:0}.el-upload-list--picture-card .el-upload-list__item:hover .el-progress__text{display:block}.el-upload-list--picture-card .el-upload-list__item .el-upload-list__item-name{display:none}.el-upload-list--picture-card .el-upload-list__item-thumbnail{height:100%;-o-object-fit:contain;object-fit:contain;width:100%}.el-upload-list--picture-card .el-upload-list__item-status-label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-list--picture-card .el-upload-list__item-status-label 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img{display:block;height:100%;width:100%}.el-upload-cover__label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-cover__label i{color:#fff;font-size:12px;margin-top:11px;transform:rotate(-45deg)}.el-upload-cover__progress{display:inline-block;position:static;vertical-align:middle;width:243px}.el-upload-cover__progress+.el-upload__inner{opacity:0}.el-upload-cover__content{height:100%;left:0;position:absolute;top:0;width:100%}.el-upload-cover__interact{background-color:var(--el-overlay-color-light);bottom:0;height:100%;left:0;position:absolute;text-align:center;width:100%}.el-upload-cover__interact .btn{color:#fff;cursor:pointer;display:inline-block;font-size:14px;margin-top:60px;transition:var(--el-transition-md-fade);vertical-align:middle}.el-upload-cover__interact .btn i{margin-top:0}.el-upload-cover__interact .btn span{opacity:0;transition:opacity .15s linear}.el-upload-cover__interact 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var(--el-border-color-light)}.el-popper.is-light>.el-popper__arrow:before{right:0}.el-popper.is-pure{padding:0}.el-popper__arrow,.el-popper__arrow:before{height:10px;position:absolute;width:10px;z-index:-1}.el-popper__arrow:before{background:var(--el-text-color-primary);box-sizing:border-box;content:" 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diff --git a/.output/public/notebook/_payload.json b/.output/public/notebook/_payload.json
index a8d8b5380e19193e7d4fa0763109b57605507476..092dd5733372b87b3141141d4beef47ea5b214ba 100644
--- a/.output/public/notebook/_payload.json
+++ b/.output/public/notebook/_payload.json
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.el-button--info:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button{--el-button-font-weight:var(--el-font-weight-primary);--el-button-border-color:var(--el-border-color);--el-button-bg-color:var(--el-fill-color-blank);--el-button-text-color:var(--el-text-color-regular);--el-button-disabled-text-color:var(--el-disabled-text-color);--el-button-disabled-bg-color:var(--el-fill-color-blank);--el-button-disabled-border-color:var(--el-border-color-light);--el-button-divide-border-color:hsla(0,0%,100%,.5);--el-button-hover-text-color:var(--el-color-primary);--el-button-hover-bg-color:var(--el-color-primary-light-9);--el-button-hover-border-color:var(--el-color-primary-light-7);--el-button-active-text-color:var(--el-button-hover-text-color);--el-button-active-border-color:var(--el-color-primary);--el-button-active-bg-color:var(--el-button-hover-bg-color);--el-button-outline-color:var(--el-color-primary-light-5);--el-button-hover-link-text-color:var(--el-color-info);--el-button-active-color:var(--el-text-color-primary);align-items:center;-webkit-appearance:none;background-color:var(--el-button-bg-color);border:var(--el-border);border-color:var(--el-button-border-color);box-sizing:border-box;color:var(--el-button-text-color);cursor:pointer;display:inline-flex;font-weight:var(--el-button-font-weight);height:32px;justify-content:center;line-height:1;outline:none;text-align:center;transition:.1s;-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-button:hover{background-color:var(--el-button-hover-bg-color);border-color:var(--el-button-hover-border-color);color:var(--el-button-hover-text-color);outline:none}.el-button:active{background-color:var(--el-button-active-bg-color);border-color:var(--el-button-active-border-color);color:var(--el-button-active-text-color);outline:none}.el-button:focus-visible{outline:2px 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1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper 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.el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags 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.el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large 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11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) 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cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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79.1666666667%;max-width:79.1666666667%}.el-col-xs-19.is-guttered{display:block}.el-col-xs-offset-19{margin-left:79.1666666667%}.el-col-xs-pull-19{position:relative;right:79.1666666667%}.el-col-xs-push-19{left:79.1666666667%;position:relative}.el-col-xs-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xs-20.is-guttered{display:block}.el-col-xs-offset-20{margin-left:83.3333333333%}.el-col-xs-pull-20{position:relative;right:83.3333333333%}.el-col-xs-push-20{left:83.3333333333%;position:relative}.el-col-xs-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xs-21.is-guttered{display:block}.el-col-xs-offset-21{margin-left:87.5%}.el-col-xs-pull-21{position:relative;right:87.5%}.el-col-xs-push-21{left:87.5%;position:relative}.el-col-xs-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 0%;max-width:0}.el-col-sm-0.is-guttered{display:none}.el-col-sm-offset-0{margin-left:0}.el-col-sm-pull-0{position:relative;right:0}.el-col-sm-push-0{left:0;position:relative}.el-col-sm-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-sm-1,.el-col-sm-1.is-guttered{display:block}.el-col-sm-offset-1{margin-left:4.1666666667%}.el-col-sm-pull-1{position:relative;right:4.1666666667%}.el-col-sm-push-1{left:4.1666666667%;position:relative}.el-col-sm-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-sm-2,.el-col-sm-2.is-guttered{display:block}.el-col-sm-offset-2{margin-left:8.3333333333%}.el-col-sm-pull-2{position:relative;right:8.3333333333%}.el-col-sm-push-2{left:8.3333333333%;position:relative}.el-col-sm-3{flex:0 0 12.5%;max-width:12.5%}.el-col-sm-3,.el-col-sm-3.is-guttered{display:block}.el-col-sm-offset-3{margin-left:12.5%}.el-col-sm-pull-3{position:relative;right:12.5%}.el-col-sm-push-3{left:12.5%;position:relative}.el-col-sm-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-sm-4,.el-col-sm-4.is-guttered{display:block}.el-col-sm-offset-4{margin-left:16.6666666667%}.el-col-sm-pull-4{position:relative;right:16.6666666667%}.el-col-sm-push-4{left:16.6666666667%;position:relative}.el-col-sm-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-sm-5,.el-col-sm-5.is-guttered{display:block}.el-col-sm-offset-5{margin-left:20.8333333333%}.el-col-sm-pull-5{position:relative;right:20.8333333333%}.el-col-sm-push-5{left:20.8333333333%;position:relative}.el-col-sm-6{flex:0 0 25%;max-width:25%}.el-col-sm-6,.el-col-sm-6.is-guttered{display:block}.el-col-sm-offset-6{margin-left:25%}.el-col-sm-pull-6{position:relative;right:25%}.el-col-sm-push-6{left:25%;position:relative}.el-col-sm-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 0%;max-width:0}.el-col-xl-0.is-guttered{display:none}.el-col-xl-offset-0{margin-left:0}.el-col-xl-pull-0{position:relative;right:0}.el-col-xl-push-0{left:0;position:relative}.el-col-xl-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-xl-1,.el-col-xl-1.is-guttered{display:block}.el-col-xl-offset-1{margin-left:4.1666666667%}.el-col-xl-pull-1{position:relative;right:4.1666666667%}.el-col-xl-push-1{left:4.1666666667%;position:relative}.el-col-xl-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-xl-2,.el-col-xl-2.is-guttered{display:block}.el-col-xl-offset-2{margin-left:8.3333333333%}.el-col-xl-pull-2{position:relative;right:8.3333333333%}.el-col-xl-push-2{left:8.3333333333%;position:relative}.el-col-xl-3{flex:0 0 12.5%;max-width:12.5%}.el-col-xl-3,.el-col-xl-3.is-guttered{display:block}.el-col-xl-offset-3{margin-left:12.5%}.el-col-xl-pull-3{position:relative;right:12.5%}.el-col-xl-push-3{left:12.5%;position:relative}.el-col-xl-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-xl-4,.el-col-xl-4.is-guttered{display:block}.el-col-xl-offset-4{margin-left:16.6666666667%}.el-col-xl-pull-4{position:relative;right:16.6666666667%}.el-col-xl-push-4{left:16.6666666667%;position:relative}.el-col-xl-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-xl-5,.el-col-xl-5.is-guttered{display:block}.el-col-xl-offset-5{margin-left:20.8333333333%}.el-col-xl-pull-5{position:relative;right:20.8333333333%}.el-col-xl-push-5{left:20.8333333333%;position:relative}.el-col-xl-6{flex:0 0 25%;max-width:25%}.el-col-xl-6,.el-col-xl-6.is-guttered{display:block}.el-col-xl-offset-6{margin-left:25%}.el-col-xl-pull-6{position:relative;right:25%}.el-col-xl-push-6{left:25%;position:relative}.el-col-xl-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-xl-10.is-guttered{display:block}.el-col-xl-offset-10{margin-left:41.6666666667%}.el-col-xl-pull-10{position:relative;right:41.6666666667%}.el-col-xl-push-10{left:41.6666666667%;position:relative}.el-col-xl-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-xl-11.is-guttered{display:block}.el-col-xl-offset-11{margin-left:45.8333333333%}.el-col-xl-pull-11{position:relative;right:45.8333333333%}.el-col-xl-push-11{left:45.8333333333%;position:relative}.el-col-xl-12{display:block;flex:0 0 50%;max-width:50%}.el-col-xl-12.is-guttered{display:block}.el-col-xl-offset-12{margin-left:50%}.el-col-xl-pull-12{position:relative;right:50%}.el-col-xl-push-12{left:50%;position:relative}.el-col-xl-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px;box-sizing:border-box;height:12px;position:relative;width:280px}.el-color-alpha-slider__bar{background:linear-gradient(to right,hsla(0,0%,100%,0) 0,var(--el-bg-color) 100%);height:100%;position:relative}.el-color-alpha-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-alpha-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-picker.is-focused .el-color-picker__trigger{border-color:var(--el-color-primary)}.el-color-picker.is-disabled .el-color-picker__trigger{cursor:not-allowed}.el-color-picker--large{height:40px}.el-color-picker--large .el-color-picker__trigger{height:40px;width:40px}.el-color-picker--large .el-color-picker__mask{height:38px;width:38px}.el-color-picker--small{height:24px}.el-color-picker--small .el-color-picker__trigger{height:24px;width:24px}.el-color-picker--small .el-color-picker__mask{height:22px;width:22px}.el-color-picker--small .el-color-picker__empty,.el-color-picker--small .el-color-picker__icon{transform:scale(.8)}.el-color-picker__mask{background-color:hsla(0,0%,100%,.7);border-radius:4px;cursor:not-allowed;height:30px;left:1px;position:absolute;top:1px;width:30px;z-index:1}.el-color-picker__trigger{align-items:center;border:1px solid var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker 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5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid 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var(--el-transition-duration)}.dialog-fade-leave-active .el-overlay-dialog{animation:dialog-fade-out var(--el-transition-duration)}@keyframes dialog-fade-in{0%{opacity:0;transform:translate3d(0,-20px,0)}to{opacity:1;transform:translateZ(0)}}@keyframes dialog-fade-out{0%{opacity:1;transform:translateZ(0)}to{opacity:0;transform:translate3d(0,-20px,0)}}@keyframes modal-fade-in{0%{opacity:0}to{opacity:1}}@keyframes modal-fade-out{0%{opacity:1}to{opacity:0}}.el-divider{position:relative}.el-divider--horizontal{border-top:1px var(--el-border-color) var(--el-border-style);display:block;height:1px;margin:24px 0;width:100%}.el-divider--vertical{border-left:1px var(--el-border-color) var(--el-border-style);display:inline-block;height:1em;margin:0 8px;position:relative;vertical-align:middle;width:1px}.el-divider__text{background-color:var(--el-bg-color);color:var(--el-text-color-primary);font-size:14px;font-weight:500;padding:0 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.ttb{transform:translate(0)}.el-drawer__sr-focus:focus{outline:none!important}.el-drawer__header{align-items:center;color:#72767b;display:flex;margin-bottom:32px;padding:var(--el-drawer-padding-primary);padding-bottom:0}.el-drawer__header>:first-child{flex:1}.el-drawer__title{flex:1;font-size:16px;line-height:inherit;margin:0}.el-drawer__footer{padding:var(--el-drawer-padding-primary);padding-top:10px;text-align:right}.el-drawer__close-btn{background-color:transparent;border:none;color:inherit;cursor:pointer;display:inline-flex;font-size:var(--el-font-size-extra-large);outline:none}.el-drawer__close-btn:focus i,.el-drawer__close-btn:hover 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.el-popper__arrow:before{border:1px solid var(--el-border-color-light)}.el-dropdown__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-dropdown__popper .el-dropdown-menu{border:none}.el-dropdown__popper .el-dropdown__popper-selfdefine{outline:none}.el-dropdown__popper .el-scrollbar__bar{z-index:calc(var(--el-dropdown-menu-index) + 1)}.el-dropdown__popper .el-dropdown__list{box-sizing:border-box;list-style:none;margin:0;padding:0}.el-dropdown 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var(--el-transition-duration);width:var(--el-notification-width);z-index:9999}.el-notification.right{right:16px}.el-notification.left{left:16px}.el-notification__group{margin-left:var(--el-notification-group-margin-left);margin-right:var(--el-notification-group-margin-right)}.el-notification__title{color:var(--el-notification-title-color);font-size:var(--el-notification-title-font-size);font-weight:700;line-height:var(--el-notification-icon-size);margin:0}.el-notification__content{color:var(--el-notification-content-color);font-size:var(--el-notification-content-font-size);line-height:24px;margin:6px 0 0}.el-notification__content p{margin:0}.el-notification .el-notification__icon{font-size:var(--el-notification-icon-size);height:var(--el-notification-icon-size);width:var(--el-notification-icon-size)}.el-notification .el-notification__closeBtn{color:var(--el-notification-close-color);cursor:pointer;font-size:var(--el-notification-close-font-size);position:absolute;right:15px;top:18px}.el-notification .el-notification__closeBtn:hover{color:var(--el-notification-close-hover-color)}.el-notification .el-notification--success{--el-notification-icon-color:var(--el-color-success);color:var(--el-notification-icon-color)}.el-notification .el-notification--info{--el-notification-icon-color:var(--el-color-info);color:var(--el-notification-icon-color)}.el-notification .el-notification--warning{--el-notification-icon-color:var(--el-color-warning);color:var(--el-notification-icon-color)}.el-notification .el-notification--error{--el-notification-icon-color:var(--el-color-error);color:var(--el-notification-icon-color)}.el-notification-fade-enter-from.right{right:0;transform:translateX(100%)}.el-notification-fade-enter-from.left{left:0;transform:translateX(-100%)}.el-notification-fade-leave-to{opacity:0}.el-overlay{background-color:var(--el-overlay-color-lighter);bottom:0;height:100%;left:0;overflow:auto;position:fixed;right:0;top:0;z-index:2000}.el-overlay .el-overlay-root{height:0}.el-page-header.is-contentful .el-page-header__main{border-top:1px solid var(--el-border-color-light);margin-top:16px}.el-page-header__header{align-items:center;display:flex;justify-content:space-between;line-height:24px}.el-page-header__left{align-items:center;display:flex;margin-right:40px;position:relative}.el-page-header__back{align-items:center;cursor:pointer;display:flex}.el-page-header__left .el-divider--vertical{margin:0 16px}.el-page-header__icon{align-items:center;display:flex;font-size:16px;margin-right:10px}.el-page-header__icon .el-icon{font-size:inherit}.el-page-header__title{font-size:14px;font-weight:500}.el-page-header__content{color:var(--el-text-color-primary);font-size:18px}.el-page-header__breadcrumb{margin-bottom:16px}.el-pagination{--el-pagination-font-size:14px;--el-pagination-bg-color:var(--el-fill-color-blank);--el-pagination-text-color:var(--el-text-color-primary);--el-pagination-border-radius:2px;--el-pagination-button-color:var(--el-text-color-primary);--el-pagination-button-width:32px;--el-pagination-button-height:32px;--el-pagination-button-disabled-color:var(--el-text-color-placeholder);--el-pagination-button-disabled-bg-color:var(--el-fill-color-blank);--el-pagination-button-bg-color:var(--el-fill-color);--el-pagination-hover-color:var(--el-color-primary);--el-pagination-font-size-small:12px;--el-pagination-button-width-small:24px;--el-pagination-button-height-small:24px;--el-pagination-button-width-large:40px;--el-pagination-button-height-large:40px;--el-pagination-item-gap:16px;align-items:center;color:var(--el-pagination-text-color);display:flex;font-size:var(--el-pagination-font-size);font-weight:400;white-space:nowrap}.el-pagination .el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small 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.el-transfer-panel__header{align-items:center;background:var(--el-transfer-panel-header-bg-color);border:1px solid var(--el-transfer-border-color);border-top-left-radius:var(--el-transfer-border-radius);border-top-right-radius:var(--el-transfer-border-radius);box-sizing:border-box;color:var(--el-color-black);display:flex;height:var(--el-transfer-panel-header-height);margin:0;padding-left:15px}.el-transfer-panel .el-transfer-panel__header .el-checkbox{align-items:center;display:flex;position:relative;width:100%}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label{color:var(--el-text-color-primary);font-size:16px;font-weight:400}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label span{color:var(--el-text-color-secondary);font-size:12px;font-weight:400;position:absolute;right:15px;top:50%;transform:translate3d(0,-50%,0)}.el-transfer-panel .el-transfer-panel__footer{background:var(--el-bg-color-overlay);border:1px solid 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0;overflow:hidden;padding:0;width:var(--el-upload-list-picture-card-size)}.el-upload-list--picture-card .el-upload-list__item .el-icon--check,.el-upload-list--picture-card .el-upload-list__item .el-icon--circle-check{color:#fff}.el-upload-list--picture-card .el-upload-list__item .el-icon--close{display:none}.el-upload-list--picture-card .el-upload-list__item:hover .el-upload-list__item-status-label{display:block;opacity:0}.el-upload-list--picture-card .el-upload-list__item:hover .el-progress__text{display:block}.el-upload-list--picture-card .el-upload-list__item .el-upload-list__item-name{display:none}.el-upload-list--picture-card .el-upload-list__item-thumbnail{height:100%;-o-object-fit:contain;object-fit:contain;width:100%}.el-upload-list--picture-card .el-upload-list__item-status-label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-list--picture-card .el-upload-list__item-status-label 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img{display:block;height:100%;width:100%}.el-upload-cover__label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-cover__label i{color:#fff;font-size:12px;margin-top:11px;transform:rotate(-45deg)}.el-upload-cover__progress{display:inline-block;position:static;vertical-align:middle;width:243px}.el-upload-cover__progress+.el-upload__inner{opacity:0}.el-upload-cover__content{height:100%;left:0;position:absolute;top:0;width:100%}.el-upload-cover__interact{background-color:var(--el-overlay-color-light);bottom:0;height:100%;left:0;position:absolute;text-align:center;width:100%}.el-upload-cover__interact .btn{color:#fff;cursor:pointer;display:inline-block;font-size:14px;margin-top:60px;transition:var(--el-transition-md-fade);vertical-align:middle}.el-upload-cover__interact .btn i{margin-top:0}.el-upload-cover__interact .btn span{opacity:0;transition:opacity .15s linear}.el-upload-cover__interact 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var(--el-border-color-light)}.el-popper.is-light>.el-popper__arrow:before{right:0}.el-popper.is-pure{padding:0}.el-popper__arrow,.el-popper__arrow:before{height:10px;position:absolute;width:10px;z-index:-1}.el-popper__arrow:before{background:var(--el-text-color-primary);box-sizing:border-box;content:" 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role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Human Practices<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li></ul><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-mobile px-10 flex lg:hidden"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 2x" class="cursor-pointer py-2" src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div class="grow"></div><!--[--><span class="el-avatar el-avatar--icon el-avatar--circle self-center cursor-pointer el-tooltip__trigger el-tooltip__trigger" style=""><i class="el-icon" style=""><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M160 448a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zM160 896a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32z"></path></svg><!--]--></i></span><!--teleport start--><!--teleport end--><!--]--></ul></header><div class="pt-[8rem]"><!--[--><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="cover" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/wetlab.webp 1x, https://static.igem.wiki/teams/5358/banner/wetlab.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/wetlab.webp" data-v-dabe85f1><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg" data-v-dabe85f1>Notebook</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10" data-v-dabe85f1><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[450px] md:mr-10" data-v-dabe85f1><h1 class="mt-0" data-v-dabe85f1>TOC</h1><ul data-v-dabe85f1><!--[--><li class="my-5" data-v-dabe85f1><a class="cursor-pointer" data-v-dabe85f1>May-June</a><ul class="hidden pl-5 pt-2" data-v-dabe85f1><!--[--><!--]--></ul></li><li class="my-5" data-v-dabe85f1><a class="cursor-pointer" data-v-dabe85f1>June-September</a><ul class="hidden pl-5 pt-2" data-v-dabe85f1><!--[--><!--[--><li class="text-[#fff] font-normal mb-2" data-v-dabe85f1><a class="cursor-pointer" data-v-dabe85f1>1. Construction of recA-BsFbFp</a></li><!--]--><!--[--><li class="text-[#fff] font-normal mb-2" data-v-dabe85f1><a class="cursor-pointer" data-v-dabe85f1>2. Construction of recA-HRP-egfp</a></li><!--]--><!--[--><li class="text-[#fff] font-normal mb-2" data-v-dabe85f1><a class="cursor-pointer" data-v-dabe85f1>3. Construction of Pj23100-LuxI and Pj23100-Plux-LuxR-egfp</a></li><!--]--><!--[--><li class="text-[#fff] font-normal mb-2" data-v-dabe85f1><a class="cursor-pointer" data-v-dabe85f1>4. Intein-mediated sequencing and ligation</a></li><!--]--><!--]--></ul></li><!--]--></ul></aside><div class="content" data-v-dabe85f1><h2 id="may-june"><!--[-->May-June<!--]--></h2><p><!--[-->The team members conducted a background survey on the effects of DNA damage and endocrine interference, and designed a specific logical gate line based on this. Our project employs the insertion of RecA intein into T7 RNAP to detect endocrine disruption effects and utilizes the SOS response mechanism of Escherichia coli to detect DNA damage. Additionally, the LuxI/R system is introduced to achieve an AND gate detection of both effects. Furthermore, the Hrp gene regulation system and BsFbFp fluorescent gene are combined to amplify the transcriptional signal and ensure effective signal expression under anaerobic conditions<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/notebook/ahl.png 1x, https://static.igem.wiki/teams/5358/notebook/ahl.png 2x" src="https://static.igem.wiki/teams/5358/notebook/ahl.png"><!--]--></p><h2 id="june-september"><!--[-->June-September<!--]--></h2><p><!--[-->The experimental verification of the constructed lines is roughly divided into four links.<!--]--></p><h3 id="_1-construction-of-reca-bsfbfp"><!--[-->1. Construction of recA-BsFbFp<!--]--></h3><div class="paren-ol"><!--[--><ol><!--[--><li><!--[-->Plasmid Mini-Preparation (Extraction of the pUC19 Plasmid)<!--]--></li><li><!--[-->PCR Amplification (Amplification of a RecA fragment of approximately 100bp)<!--]--></li><li><!--[-->Enzymatic Digestion (Digestion of the plasmid containing BsFbFP with different restriction enzymes)<!--]--></li><li><!--[-->Agarose Gel Electrophoresis (Detection of the target fragment for subsequent recovery)<!--]--></li><li><!--[-->Gel Extraction (Recovery of the target fragment from the agarose gel)<!--]--></li><li><!--[-->DNA Ligase Mediated Joining of DNA Fragments (Ligation to construct the recombinant plasmid)<!--]--></li><!--]--></ol><!--]--></div><h3 id="_2-construction-of-reca-hrp-egfp"><!--[-->2. Construction of recA-HRP-egfp<!--]--></h3><div class="paren-ol"><!--[--><ol><!--[--><li><!--[-->Plasmid Mini-Preparation (Extraction of the pET28 plasmid)<!--]--></li><li><!--[-->PCR Amplification (Amplification of a RecA fragment of approximately 100bp)<!--]--></li><li><!--[-->Enzymatic Digestion (Digestion of the plasmid and PCR product with restriction enzymes)<!--]--></li><li><!--[-->Agarose Gel Electrophoresis (Detection of the target fragment for subsequent recovery)<!--]--></li><li><!--[-->Gel Extraction (Recovery of the target fragment from the agarose gel)<!--]--></li><li><!--[-->DNA Ligase Mediated Joining of DNA Fragments  (Ligation of recA with pET28)<!--]--></li><!--]--></ol><!--]--></div><h3 id="_3-construction-of-pj23100-luxi-and-pj23100-plux-luxr-egfp"><!--[-->3. Construction of Pj23100-LuxI and Pj23100-Plux-LuxR-egfp<!--]--></h3><div class="paren-ol"><!--[--><ol><!--[--><li><!--[-->Primer Design<!--]--></li><li><!--[-->PCR Amplification (Individual PCR amplification of Pj23100, LuxI, Pj23100-Plux-LuxR, and egfp)<!--]--></li><li><!--[-->Enzymatic Digestion (Digestion of the plasmid and PCR products with restriction enzymes)<!--]--></li><li><!--[-->Agarose Gel Electrophoresis (Detection of the target fragments for subsequent recovery)<!--]--></li><li><!--[-->Gel Extraction (Recovery of the target fragments from the agarose gel)<!--]--></li><li><!--[-->DNA Ligase Mediated Joining of DNA Fragments (Ligation of the target fragments with the plasmid)<!--]--></li><!--]--></ol><!--]--></div><h3 id="_4-intein-mediated-sequencing-and-ligation"><!--[-->4. Intein-mediated sequencing and ligation<!--]--></h3><h4 id="_623-626"><!--[-->6.23-6.26<!--]--></h4><p><!--[-->The initial attempt at constructing recA-BsFbFp was made, with successful enzymatic digestion but no bacterial colonies grown on the solid medium after ligation and cultivation. The failure may have been due to incorrect sample addition during gel extraction.<!--]--></p><h4 id="_626-630"><!--[-->6.26-6.30<!--]--></h4><p><!--[-->The experiment was repeated, following the same process as the first attempt, but again no colonies were grown, possibly due to insufficient plasmid concentration leading to failure.<!--]--></p><h4 id="_72-75"><!--[-->7.2-7.5<!--]--></h4><p><!--[-->The construction process of recA-BsFbFp was repeated, but no plasmid bands were observed after electrophoresis, which is likely due to low concentration; simultaneously, the first construction of recA-HRP-egfp was initiated.<!--]--></p><h4 id="_76-710"><!--[-->7.6-7.10<!--]--></h4><p><!--[-->The recA-BsFbFp construction was attempted again. The step of plasmid miniprep was missed on the seventh step, which might have allowed nucleases to degrade the plasmid, resulting in no bands on the electrophoresis and a failed experiment; the second construction of recA-HRP-egfp was conducted.<!--]--></p><h4 id="_710-713"><!--[-->7.10-7.13<!--]--></h4><p><!--[-->The construction of recA-BsFbFp was successful this time, allowing for subsequent characterization experiments to be carried out; the third construction of recA-HRP-egfp was undertaken.<!--]--></p><h4 id="_713-724"><!--[-->7.13-7.24<!--]--></h4><p><!--[-->Characterization of the successfully constructed recA-BsFbFp was performed, with a cycle every two days for a total of six cycles, and three of them were selected for validation. During this period, the fourth construction of recA-HRP-egfp was attempted, but the results were not satisfactory, with all being vector self-ligation. Therefore, reasons were sought, improvements were made, and it was ultimately decided to change the ligation system.<!--]--></p><h4 id="_724-827"><!--[-->7.24-8.27<!--]--></h4><p><!--[-->The original ligation system was based on a 1:4 ratio of vector to insert. The experiment was continued with the same ratio and then repeated with ratios of 1:3, 1:5, 1:6, and 1:9, but the construction was still unsuccessful.<!--]--></p><h4 id="_828-914"><!--[-->8.28-9.14<!--]--></h4><p><!--[-->The construction of Pj23100-LuxI and Pj23100-Plux-LuxR-egfp was initiated, while still searching for the reasons behind the failure of recA-HRP-egfp construction. It was then decided to change the primers, adding protective bases at both ends of the primers&#39; restriction sites.<!--]--></p><h4 id="_915-929"><!--[-->9.15-9.29<!--]--></h4><p><!--[-->Intein-mediated sequencing and ligation were performed, while continuously changing the primers, increasing the protective bases from one pair to three pairs, and repeating the construction of recA-HRP-egfp, but without success.<!--]--></p><p><!--[-->Ultimately, due to time constraints, the construction of recA-HRP-egfp was not successful, which prevented validation from being completed.<!--]--></p></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/notebook/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-IarcltrcI1":11},null,"/notebook",1727868994393]</script>
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role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Human Practices<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li></ul><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-mobile px-10 flex lg:hidden"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 2x" class="cursor-pointer py-2" src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div class="grow"></div><!--[--><span class="el-avatar el-avatar--icon el-avatar--circle self-center cursor-pointer el-tooltip__trigger el-tooltip__trigger" style=""><i class="el-icon" style=""><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M160 448a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zM160 896a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32z"></path></svg><!--]--></i></span><!--teleport start--><!--teleport end--><!--]--></ul></header><div class="pt-[8rem]"><!--[--><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="cover" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/wetlab.webp 1x, https://static.igem.wiki/teams/5358/banner/wetlab.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/wetlab.webp" data-v-dabe85f1><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg" data-v-dabe85f1>Notebook</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10" data-v-dabe85f1><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[450px] md:mr-10" data-v-dabe85f1><h1 class="mt-0" data-v-dabe85f1>TOC</h1><ul data-v-dabe85f1><!--[--><li class="my-5" data-v-dabe85f1><a class="cursor-pointer" data-v-dabe85f1>May-June</a><ul class="hidden pl-5 pt-2" data-v-dabe85f1><!--[--><!--]--></ul></li><li class="my-5" data-v-dabe85f1><a class="cursor-pointer" data-v-dabe85f1>June-September</a><ul class="hidden pl-5 pt-2" data-v-dabe85f1><!--[--><!--[--><li class="text-[#fff] font-normal mb-2" data-v-dabe85f1><a class="cursor-pointer" data-v-dabe85f1>1. Construction of recA-BsFbFp</a></li><!--]--><!--[--><li class="text-[#fff] font-normal mb-2" data-v-dabe85f1><a class="cursor-pointer" data-v-dabe85f1>2. Construction of recA-HRP-egfp</a></li><!--]--><!--[--><li class="text-[#fff] font-normal mb-2" data-v-dabe85f1><a class="cursor-pointer" data-v-dabe85f1>3. Construction of Pj23100-LuxI and Pj23100-Plux-LuxR-egfp</a></li><!--]--><!--[--><li class="text-[#fff] font-normal mb-2" data-v-dabe85f1><a class="cursor-pointer" data-v-dabe85f1>4. Intein-mediated sequencing and ligation</a></li><!--]--><!--]--></ul></li><!--]--></ul></aside><div class="content" data-v-dabe85f1><h2 id="may-june"><!--[-->May-June<!--]--></h2><p><!--[-->The team members conducted a background survey on the effects of DNA damage and endocrine interference, and designed a specific logical gate line based on this. Our project employs the insertion of RecA intein into T7 RNAP to detect endocrine disruption effects and utilizes the SOS response mechanism of Escherichia coli to detect DNA damage. Additionally, the LuxI/R system is introduced to achieve an AND gate detection of both effects. Furthermore, the Hrp gene regulation system and BsFbFp fluorescent gene are combined to amplify the transcriptional signal and ensure effective signal expression under anaerobic conditions<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/notebook/ahl.png 1x, https://static.igem.wiki/teams/5358/notebook/ahl.png 2x" src="https://static.igem.wiki/teams/5358/notebook/ahl.png"><!--]--></p><h2 id="june-september"><!--[-->June-September<!--]--></h2><p><!--[-->The experimental verification of the constructed lines is roughly divided into four links.<!--]--></p><h3 id="_1-construction-of-reca-bsfbfp"><!--[-->1. Construction of recA-BsFbFp<!--]--></h3><div class="paren-ol"><!--[--><ol><!--[--><li><!--[-->Plasmid Mini-Preparation (Extraction of the pUC19 Plasmid)<!--]--></li><li><!--[-->PCR Amplification (Amplification of a RecA fragment of approximately 100bp)<!--]--></li><li><!--[-->Enzymatic Digestion (Digestion of the plasmid containing BsFbFP with different restriction enzymes)<!--]--></li><li><!--[-->Agarose Gel Electrophoresis (Detection of the target fragment for subsequent recovery)<!--]--></li><li><!--[-->Gel Extraction (Recovery of the target fragment from the agarose gel)<!--]--></li><li><!--[-->DNA Ligase Mediated Joining of DNA Fragments (Ligation to construct the recombinant plasmid)<!--]--></li><!--]--></ol><!--]--></div><h3 id="_2-construction-of-reca-hrp-egfp"><!--[-->2. Construction of recA-HRP-egfp<!--]--></h3><div class="paren-ol"><!--[--><ol><!--[--><li><!--[-->Plasmid Mini-Preparation (Extraction of the pET28 plasmid)<!--]--></li><li><!--[-->PCR Amplification (Amplification of a RecA fragment of approximately 100bp)<!--]--></li><li><!--[-->Enzymatic Digestion (Digestion of the plasmid and PCR product with restriction enzymes)<!--]--></li><li><!--[-->Agarose Gel Electrophoresis (Detection of the target fragment for subsequent recovery)<!--]--></li><li><!--[-->Gel Extraction (Recovery of the target fragment from the agarose gel)<!--]--></li><li><!--[-->DNA Ligase Mediated Joining of DNA Fragments  (Ligation of recA with pET28)<!--]--></li><!--]--></ol><!--]--></div><h3 id="_3-construction-of-pj23100-luxi-and-pj23100-plux-luxr-egfp"><!--[-->3. Construction of Pj23100-LuxI and Pj23100-Plux-LuxR-egfp<!--]--></h3><div class="paren-ol"><!--[--><ol><!--[--><li><!--[-->Primer Design<!--]--></li><li><!--[-->PCR Amplification (Individual PCR amplification of Pj23100, LuxI, Pj23100-Plux-LuxR, and egfp)<!--]--></li><li><!--[-->Enzymatic Digestion (Digestion of the plasmid and PCR products with restriction enzymes)<!--]--></li><li><!--[-->Agarose Gel Electrophoresis (Detection of the target fragments for subsequent recovery)<!--]--></li><li><!--[-->Gel Extraction (Recovery of the target fragments from the agarose gel)<!--]--></li><li><!--[-->DNA Ligase Mediated Joining of DNA Fragments (Ligation of the target fragments with the plasmid)<!--]--></li><!--]--></ol><!--]--></div><h3 id="_4-intein-mediated-sequencing-and-ligation"><!--[-->4. Intein-mediated sequencing and ligation<!--]--></h3><h4 id="_623-626"><!--[-->6.23-6.26<!--]--></h4><p><!--[-->The initial attempt at constructing recA-BsFbFp was made, with successful enzymatic digestion but no bacterial colonies grown on the solid medium after ligation and cultivation. The failure may have been due to incorrect sample addition during gel extraction.<!--]--></p><h4 id="_626-630"><!--[-->6.26-6.30<!--]--></h4><p><!--[-->The experiment was repeated, following the same process as the first attempt, but again no colonies were grown, possibly due to insufficient plasmid concentration leading to failure.<!--]--></p><h4 id="_72-75"><!--[-->7.2-7.5<!--]--></h4><p><!--[-->The construction process of recA-BsFbFp was repeated, but no plasmid bands were observed after electrophoresis, which is likely due to low concentration; simultaneously, the first construction of recA-HRP-egfp was initiated.<!--]--></p><h4 id="_76-710"><!--[-->7.6-7.10<!--]--></h4><p><!--[-->The recA-BsFbFp construction was attempted again. The step of plasmid miniprep was missed on the seventh step, which might have allowed nucleases to degrade the plasmid, resulting in no bands on the electrophoresis and a failed experiment; the second construction of recA-HRP-egfp was conducted.<!--]--></p><h4 id="_710-713"><!--[-->7.10-7.13<!--]--></h4><p><!--[-->The construction of recA-BsFbFp was successful this time, allowing for subsequent characterization experiments to be carried out; the third construction of recA-HRP-egfp was undertaken.<!--]--></p><h4 id="_713-724"><!--[-->7.13-7.24<!--]--></h4><p><!--[-->Characterization of the successfully constructed recA-BsFbFp was performed, with a cycle every two days for a total of six cycles, and three of them were selected for validation. During this period, the fourth construction of recA-HRP-egfp was attempted, but the results were not satisfactory, with all being vector self-ligation. Therefore, reasons were sought, improvements were made, and it was ultimately decided to change the ligation system.<!--]--></p><h4 id="_724-827"><!--[-->7.24-8.27<!--]--></h4><p><!--[-->The original ligation system was based on a 1:4 ratio of vector to insert. The experiment was continued with the same ratio and then repeated with ratios of 1:3, 1:5, 1:6, and 1:9, but the construction was still unsuccessful.<!--]--></p><h4 id="_828-914"><!--[-->8.28-9.14<!--]--></h4><p><!--[-->The construction of Pj23100-LuxI and Pj23100-Plux-LuxR-egfp was initiated, while still searching for the reasons behind the failure of recA-HRP-egfp construction. It was then decided to change the primers, adding protective bases at both ends of the primers&#39; restriction sites.<!--]--></p><h4 id="_915-929"><!--[-->9.15-9.29<!--]--></h4><p><!--[-->Intein-mediated sequencing and ligation were performed, while continuously changing the primers, increasing the protective bases from one pair to three pairs, and repeating the construction of recA-HRP-egfp, but without success.<!--]--></p><p><!--[-->Ultimately, due to time constraints, the construction of recA-HRP-egfp was not successful, which prevented validation from being completed.<!--]--></p></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/notebook/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-IarcltrcI1":11},null,"/notebook",1727871091263]</script>
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diff --git a/.output/public/parts/_payload.json b/.output/public/parts/_payload.json
index efe13eb8923756edbba1d47b932169836de781c5..fd25b4d5f0e287d07caf2969349c124c5e4a0ca0 100644
--- a/.output/public/parts/_payload.json
+++ b/.output/public/parts/_payload.json
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.el-button--info:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button{--el-button-font-weight:var(--el-font-weight-primary);--el-button-border-color:var(--el-border-color);--el-button-bg-color:var(--el-fill-color-blank);--el-button-text-color:var(--el-text-color-regular);--el-button-disabled-text-color:var(--el-disabled-text-color);--el-button-disabled-bg-color:var(--el-fill-color-blank);--el-button-disabled-border-color:var(--el-border-color-light);--el-button-divide-border-color:hsla(0,0%,100%,.5);--el-button-hover-text-color:var(--el-color-primary);--el-button-hover-bg-color:var(--el-color-primary-light-9);--el-button-hover-border-color:var(--el-color-primary-light-7);--el-button-active-text-color:var(--el-button-hover-text-color);--el-button-active-border-color:var(--el-color-primary);--el-button-active-bg-color:var(--el-button-hover-bg-color);--el-button-outline-color:var(--el-color-primary-light-5);--el-button-hover-link-text-color:var(--el-color-info);--el-button-active-color:var(--el-text-color-primary);align-items:center;-webkit-appearance:none;background-color:var(--el-button-bg-color);border:var(--el-border);border-color:var(--el-button-border-color);box-sizing:border-box;color:var(--el-button-text-color);cursor:pointer;display:inline-flex;font-weight:var(--el-button-font-weight);height:32px;justify-content:center;line-height:1;outline:none;text-align:center;transition:.1s;-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-button:hover{background-color:var(--el-button-hover-bg-color);border-color:var(--el-button-hover-border-color);color:var(--el-button-hover-text-color);outline:none}.el-button:active{background-color:var(--el-button-active-bg-color);border-color:var(--el-button-active-border-color);color:var(--el-button-active-text-color);outline:none}.el-button:focus-visible{outline:2px 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1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper 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.el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags 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.el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large 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11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) 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cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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79.1666666667%;max-width:79.1666666667%}.el-col-xs-19.is-guttered{display:block}.el-col-xs-offset-19{margin-left:79.1666666667%}.el-col-xs-pull-19{position:relative;right:79.1666666667%}.el-col-xs-push-19{left:79.1666666667%;position:relative}.el-col-xs-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xs-20.is-guttered{display:block}.el-col-xs-offset-20{margin-left:83.3333333333%}.el-col-xs-pull-20{position:relative;right:83.3333333333%}.el-col-xs-push-20{left:83.3333333333%;position:relative}.el-col-xs-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xs-21.is-guttered{display:block}.el-col-xs-offset-21{margin-left:87.5%}.el-col-xs-pull-21{position:relative;right:87.5%}.el-col-xs-push-21{left:87.5%;position:relative}.el-col-xs-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 0%;max-width:0}.el-col-sm-0.is-guttered{display:none}.el-col-sm-offset-0{margin-left:0}.el-col-sm-pull-0{position:relative;right:0}.el-col-sm-push-0{left:0;position:relative}.el-col-sm-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-sm-1,.el-col-sm-1.is-guttered{display:block}.el-col-sm-offset-1{margin-left:4.1666666667%}.el-col-sm-pull-1{position:relative;right:4.1666666667%}.el-col-sm-push-1{left:4.1666666667%;position:relative}.el-col-sm-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-sm-2,.el-col-sm-2.is-guttered{display:block}.el-col-sm-offset-2{margin-left:8.3333333333%}.el-col-sm-pull-2{position:relative;right:8.3333333333%}.el-col-sm-push-2{left:8.3333333333%;position:relative}.el-col-sm-3{flex:0 0 12.5%;max-width:12.5%}.el-col-sm-3,.el-col-sm-3.is-guttered{display:block}.el-col-sm-offset-3{margin-left:12.5%}.el-col-sm-pull-3{position:relative;right:12.5%}.el-col-sm-push-3{left:12.5%;position:relative}.el-col-sm-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-sm-4,.el-col-sm-4.is-guttered{display:block}.el-col-sm-offset-4{margin-left:16.6666666667%}.el-col-sm-pull-4{position:relative;right:16.6666666667%}.el-col-sm-push-4{left:16.6666666667%;position:relative}.el-col-sm-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-sm-5,.el-col-sm-5.is-guttered{display:block}.el-col-sm-offset-5{margin-left:20.8333333333%}.el-col-sm-pull-5{position:relative;right:20.8333333333%}.el-col-sm-push-5{left:20.8333333333%;position:relative}.el-col-sm-6{flex:0 0 25%;max-width:25%}.el-col-sm-6,.el-col-sm-6.is-guttered{display:block}.el-col-sm-offset-6{margin-left:25%}.el-col-sm-pull-6{position:relative;right:25%}.el-col-sm-push-6{left:25%;position:relative}.el-col-sm-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 0%;max-width:0}.el-col-xl-0.is-guttered{display:none}.el-col-xl-offset-0{margin-left:0}.el-col-xl-pull-0{position:relative;right:0}.el-col-xl-push-0{left:0;position:relative}.el-col-xl-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-xl-1,.el-col-xl-1.is-guttered{display:block}.el-col-xl-offset-1{margin-left:4.1666666667%}.el-col-xl-pull-1{position:relative;right:4.1666666667%}.el-col-xl-push-1{left:4.1666666667%;position:relative}.el-col-xl-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-xl-2,.el-col-xl-2.is-guttered{display:block}.el-col-xl-offset-2{margin-left:8.3333333333%}.el-col-xl-pull-2{position:relative;right:8.3333333333%}.el-col-xl-push-2{left:8.3333333333%;position:relative}.el-col-xl-3{flex:0 0 12.5%;max-width:12.5%}.el-col-xl-3,.el-col-xl-3.is-guttered{display:block}.el-col-xl-offset-3{margin-left:12.5%}.el-col-xl-pull-3{position:relative;right:12.5%}.el-col-xl-push-3{left:12.5%;position:relative}.el-col-xl-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-xl-4,.el-col-xl-4.is-guttered{display:block}.el-col-xl-offset-4{margin-left:16.6666666667%}.el-col-xl-pull-4{position:relative;right:16.6666666667%}.el-col-xl-push-4{left:16.6666666667%;position:relative}.el-col-xl-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-xl-5,.el-col-xl-5.is-guttered{display:block}.el-col-xl-offset-5{margin-left:20.8333333333%}.el-col-xl-pull-5{position:relative;right:20.8333333333%}.el-col-xl-push-5{left:20.8333333333%;position:relative}.el-col-xl-6{flex:0 0 25%;max-width:25%}.el-col-xl-6,.el-col-xl-6.is-guttered{display:block}.el-col-xl-offset-6{margin-left:25%}.el-col-xl-pull-6{position:relative;right:25%}.el-col-xl-push-6{left:25%;position:relative}.el-col-xl-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-xl-10.is-guttered{display:block}.el-col-xl-offset-10{margin-left:41.6666666667%}.el-col-xl-pull-10{position:relative;right:41.6666666667%}.el-col-xl-push-10{left:41.6666666667%;position:relative}.el-col-xl-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-xl-11.is-guttered{display:block}.el-col-xl-offset-11{margin-left:45.8333333333%}.el-col-xl-pull-11{position:relative;right:45.8333333333%}.el-col-xl-push-11{left:45.8333333333%;position:relative}.el-col-xl-12{display:block;flex:0 0 50%;max-width:50%}.el-col-xl-12.is-guttered{display:block}.el-col-xl-offset-12{margin-left:50%}.el-col-xl-pull-12{position:relative;right:50%}.el-col-xl-push-12{left:50%;position:relative}.el-col-xl-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px;box-sizing:border-box;height:12px;position:relative;width:280px}.el-color-alpha-slider__bar{background:linear-gradient(to right,hsla(0,0%,100%,0) 0,var(--el-bg-color) 100%);height:100%;position:relative}.el-color-alpha-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-alpha-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-picker.is-focused .el-color-picker__trigger{border-color:var(--el-color-primary)}.el-color-picker.is-disabled .el-color-picker__trigger{cursor:not-allowed}.el-color-picker--large{height:40px}.el-color-picker--large .el-color-picker__trigger{height:40px;width:40px}.el-color-picker--large .el-color-picker__mask{height:38px;width:38px}.el-color-picker--small{height:24px}.el-color-picker--small .el-color-picker__trigger{height:24px;width:24px}.el-color-picker--small .el-color-picker__mask{height:22px;width:22px}.el-color-picker--small .el-color-picker__empty,.el-color-picker--small .el-color-picker__icon{transform:scale(.8)}.el-color-picker__mask{background-color:hsla(0,0%,100%,.7);border-radius:4px;cursor:not-allowed;height:30px;left:1px;position:absolute;top:1px;width:30px;z-index:1}.el-color-picker__trigger{align-items:center;border:1px solid var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker 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5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid 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var(--el-transition-duration)}.dialog-fade-leave-active .el-overlay-dialog{animation:dialog-fade-out var(--el-transition-duration)}@keyframes dialog-fade-in{0%{opacity:0;transform:translate3d(0,-20px,0)}to{opacity:1;transform:translateZ(0)}}@keyframes dialog-fade-out{0%{opacity:1;transform:translateZ(0)}to{opacity:0;transform:translate3d(0,-20px,0)}}@keyframes modal-fade-in{0%{opacity:0}to{opacity:1}}@keyframes modal-fade-out{0%{opacity:1}to{opacity:0}}.el-divider{position:relative}.el-divider--horizontal{border-top:1px var(--el-border-color) var(--el-border-style);display:block;height:1px;margin:24px 0;width:100%}.el-divider--vertical{border-left:1px var(--el-border-color) var(--el-border-style);display:inline-block;height:1em;margin:0 8px;position:relative;vertical-align:middle;width:1px}.el-divider__text{background-color:var(--el-bg-color);color:var(--el-text-color-primary);font-size:14px;font-weight:500;padding:0 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.ttb{transform:translate(0)}.el-drawer__sr-focus:focus{outline:none!important}.el-drawer__header{align-items:center;color:#72767b;display:flex;margin-bottom:32px;padding:var(--el-drawer-padding-primary);padding-bottom:0}.el-drawer__header>:first-child{flex:1}.el-drawer__title{flex:1;font-size:16px;line-height:inherit;margin:0}.el-drawer__footer{padding:var(--el-drawer-padding-primary);padding-top:10px;text-align:right}.el-drawer__close-btn{background-color:transparent;border:none;color:inherit;cursor:pointer;display:inline-flex;font-size:var(--el-font-size-extra-large);outline:none}.el-drawer__close-btn:focus i,.el-drawer__close-btn:hover 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.el-popper__arrow:before{border:1px solid var(--el-border-color-light)}.el-dropdown__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-dropdown__popper .el-dropdown-menu{border:none}.el-dropdown__popper .el-dropdown__popper-selfdefine{outline:none}.el-dropdown__popper .el-scrollbar__bar{z-index:calc(var(--el-dropdown-menu-index) + 1)}.el-dropdown__popper .el-dropdown__list{box-sizing:border-box;list-style:none;margin:0;padding:0}.el-dropdown 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var(--el-transition-duration);width:var(--el-notification-width);z-index:9999}.el-notification.right{right:16px}.el-notification.left{left:16px}.el-notification__group{margin-left:var(--el-notification-group-margin-left);margin-right:var(--el-notification-group-margin-right)}.el-notification__title{color:var(--el-notification-title-color);font-size:var(--el-notification-title-font-size);font-weight:700;line-height:var(--el-notification-icon-size);margin:0}.el-notification__content{color:var(--el-notification-content-color);font-size:var(--el-notification-content-font-size);line-height:24px;margin:6px 0 0}.el-notification__content p{margin:0}.el-notification .el-notification__icon{font-size:var(--el-notification-icon-size);height:var(--el-notification-icon-size);width:var(--el-notification-icon-size)}.el-notification .el-notification__closeBtn{color:var(--el-notification-close-color);cursor:pointer;font-size:var(--el-notification-close-font-size);position:absolute;right:15px;top:18px}.el-notification .el-notification__closeBtn:hover{color:var(--el-notification-close-hover-color)}.el-notification .el-notification--success{--el-notification-icon-color:var(--el-color-success);color:var(--el-notification-icon-color)}.el-notification .el-notification--info{--el-notification-icon-color:var(--el-color-info);color:var(--el-notification-icon-color)}.el-notification .el-notification--warning{--el-notification-icon-color:var(--el-color-warning);color:var(--el-notification-icon-color)}.el-notification .el-notification--error{--el-notification-icon-color:var(--el-color-error);color:var(--el-notification-icon-color)}.el-notification-fade-enter-from.right{right:0;transform:translateX(100%)}.el-notification-fade-enter-from.left{left:0;transform:translateX(-100%)}.el-notification-fade-leave-to{opacity:0}.el-overlay{background-color:var(--el-overlay-color-lighter);bottom:0;height:100%;left:0;overflow:auto;position:fixed;right:0;top:0;z-index:2000}.el-overlay .el-overlay-root{height:0}.el-page-header.is-contentful .el-page-header__main{border-top:1px solid var(--el-border-color-light);margin-top:16px}.el-page-header__header{align-items:center;display:flex;justify-content:space-between;line-height:24px}.el-page-header__left{align-items:center;display:flex;margin-right:40px;position:relative}.el-page-header__back{align-items:center;cursor:pointer;display:flex}.el-page-header__left .el-divider--vertical{margin:0 16px}.el-page-header__icon{align-items:center;display:flex;font-size:16px;margin-right:10px}.el-page-header__icon .el-icon{font-size:inherit}.el-page-header__title{font-size:14px;font-weight:500}.el-page-header__content{color:var(--el-text-color-primary);font-size:18px}.el-page-header__breadcrumb{margin-bottom:16px}.el-pagination{--el-pagination-font-size:14px;--el-pagination-bg-color:var(--el-fill-color-blank);--el-pagination-text-color:var(--el-text-color-primary);--el-pagination-border-radius:2px;--el-pagination-button-color:var(--el-text-color-primary);--el-pagination-button-width:32px;--el-pagination-button-height:32px;--el-pagination-button-disabled-color:var(--el-text-color-placeholder);--el-pagination-button-disabled-bg-color:var(--el-fill-color-blank);--el-pagination-button-bg-color:var(--el-fill-color);--el-pagination-hover-color:var(--el-color-primary);--el-pagination-font-size-small:12px;--el-pagination-button-width-small:24px;--el-pagination-button-height-small:24px;--el-pagination-button-width-large:40px;--el-pagination-button-height-large:40px;--el-pagination-item-gap:16px;align-items:center;color:var(--el-pagination-text-color);display:flex;font-size:var(--el-pagination-font-size);font-weight:400;white-space:nowrap}.el-pagination .el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small 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.el-transfer-panel__header{align-items:center;background:var(--el-transfer-panel-header-bg-color);border:1px solid var(--el-transfer-border-color);border-top-left-radius:var(--el-transfer-border-radius);border-top-right-radius:var(--el-transfer-border-radius);box-sizing:border-box;color:var(--el-color-black);display:flex;height:var(--el-transfer-panel-header-height);margin:0;padding-left:15px}.el-transfer-panel .el-transfer-panel__header .el-checkbox{align-items:center;display:flex;position:relative;width:100%}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label{color:var(--el-text-color-primary);font-size:16px;font-weight:400}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label span{color:var(--el-text-color-secondary);font-size:12px;font-weight:400;position:absolute;right:15px;top:50%;transform:translate3d(0,-50%,0)}.el-transfer-panel .el-transfer-panel__footer{background:var(--el-bg-color-overlay);border:1px solid 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0;overflow:hidden;padding:0;width:var(--el-upload-list-picture-card-size)}.el-upload-list--picture-card .el-upload-list__item .el-icon--check,.el-upload-list--picture-card .el-upload-list__item .el-icon--circle-check{color:#fff}.el-upload-list--picture-card .el-upload-list__item .el-icon--close{display:none}.el-upload-list--picture-card .el-upload-list__item:hover .el-upload-list__item-status-label{display:block;opacity:0}.el-upload-list--picture-card .el-upload-list__item:hover .el-progress__text{display:block}.el-upload-list--picture-card .el-upload-list__item .el-upload-list__item-name{display:none}.el-upload-list--picture-card .el-upload-list__item-thumbnail{height:100%;-o-object-fit:contain;object-fit:contain;width:100%}.el-upload-list--picture-card .el-upload-list__item-status-label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-list--picture-card .el-upload-list__item-status-label 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img{display:block;height:100%;width:100%}.el-upload-cover__label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-cover__label i{color:#fff;font-size:12px;margin-top:11px;transform:rotate(-45deg)}.el-upload-cover__progress{display:inline-block;position:static;vertical-align:middle;width:243px}.el-upload-cover__progress+.el-upload__inner{opacity:0}.el-upload-cover__content{height:100%;left:0;position:absolute;top:0;width:100%}.el-upload-cover__interact{background-color:var(--el-overlay-color-light);bottom:0;height:100%;left:0;position:absolute;text-align:center;width:100%}.el-upload-cover__interact .btn{color:#fff;cursor:pointer;display:inline-block;font-size:14px;margin-top:60px;transition:var(--el-transition-md-fade);vertical-align:middle}.el-upload-cover__interact .btn i{margin-top:0}.el-upload-cover__interact .btn span{opacity:0;transition:opacity .15s linear}.el-upload-cover__interact 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var(--el-border-color-light)}.el-popper.is-light>.el-popper__arrow:before{right:0}.el-popper.is-pure{padding:0}.el-popper__arrow,.el-popper__arrow:before{height:10px;position:absolute;width:10px;z-index:-1}.el-popper__arrow:before{background:var(--el-text-color-primary);box-sizing:border-box;content:" 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start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Wet Lab<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Dry Lab<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Team<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Human Practices<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li></ul><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-mobile px-10 flex lg:hidden"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 2x" class="cursor-pointer py-2" src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div class="grow"></div><!--[--><span class="el-avatar el-avatar--icon el-avatar--circle self-center cursor-pointer el-tooltip__trigger el-tooltip__trigger" style=""><i class="el-icon" style=""><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M160 448a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zM160 896a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32z"></path></svg><!--]--></i></span><!--teleport start--><!--teleport end--><!--]--></ul></header><div class="pt-[8rem]"><!--[--><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="cover" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/wetlab.webp 1x, https://static.igem.wiki/teams/5358/banner/wetlab.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/wetlab.webp" data-v-1b4324c9><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg" data-v-1b4324c9>Parts</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10" data-v-1b4324c9><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[200px] md:mr-10" data-v-1b4324c9><h1 class="mt-0" data-v-1b4324c9>TOC</h1><ul data-v-1b4324c9><!--[--><li class="my-5" data-v-1b4324c9><a class="cursor-pointer" data-v-1b4324c9>Basic Parts</a></li><li class="my-5" data-v-1b4324c9><a class="cursor-pointer" data-v-1b4324c9>Composite Parts</a></li><li class="my-5" data-v-1b4324c9><a class="cursor-pointer" data-v-1b4324c9>Reference Parts:</a></li><!--]--></ul></aside><div class="content" data-v-1b4324c9><p><!--[-->Team BIT registered 2 basic parts and  8 new composite parts.<!--]--></p><p><!--[-->The sequence source and construction method of the composite part are demonstrated in the Experiments section and Results section. Please click on the Part Name to shift to the registry for viewing further information.<!--]--></p><h2 id="basic-parts"><!--[-->Basic Parts<!--]--></h2><table><!--[--><thead><!--[--><tr><!--[--><th><!--[-->Part Code<!--]--></th><th><!--[-->Part Name<!--]--></th><th><!--[-->Type<!--]--></th><!--]--></tr><!--]--></thead><tbody><!--[--><tr><!--[--><td><!--[-->BBa_K5358010<!--]--></td><td><!--[-->BsFbFp<!--]--></td><td><!--[-->Reporter<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_K5358011<!--]--></td><td><!--[-->(N)T7RNAP-ERintein-(C)T7RNAP<!--]--></td><td><!--[-->Coding<!--]--></td><!--]--></tr><!--]--></tbody><!--]--></table><h2 id="composite-parts"><!--[-->Composite Parts<!--]--></h2><h3 id="preca-hrpr-t"><!--[-->PrecA-hrpR-T<!--]--></h3><p><!--[-->When cells are subjected to oxidative damage, PrecA initiates the expression of the downstream hrpR gene.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image1.png 1x, https://static.igem.wiki/teams/5358/parts/image1.png 2x" src="https://static.igem.wiki/teams/5358/parts/image1.png"><!--]--></p><h3 id="pj23100-luxr-t"><!--[-->Pj23100-LuxR-T<!--]--></h3><p><!--[-->Pj23100 is a constitutive promoter that enables the constitutive expression of LuxR.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image2.png 1x, https://static.igem.wiki/teams/5358/parts/image2.png 2x" src="https://static.igem.wiki/teams/5358/parts/image2.png"><!--]--></p><h3 id="pluxr-hrps-t"><!--[-->PluxR-hrpS-T<!--]--></h3><p><!--[-->Used for the constitutive regulation circuit with LuxR.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image3.png 1x, https://static.igem.wiki/teams/5358/parts/image3.png 2x" src="https://static.igem.wiki/teams/5358/parts/image3.png"><!--]--></p><h3 id="phrpl-bsfbfp-t"><!--[-->PhrpL-BsFbFp-T<!--]--></h3><p><!--[-->Used for the constitutive regulation characterization project with hrpR.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image4.png 1x, https://static.igem.wiki/teams/5358/parts/image4.png 2x" src="https://static.igem.wiki/teams/5358/parts/image4.png"><!--]--></p><h3 id="pgapa-n-t7rnap-er-lbd-intein-c-t7rnap-t"><!--[-->PgapA-N-T7RNAP-ER-LBD intein-C-T7RNAP-T<!--]--></h3><p><!--[-->PgapA is a constitutive promoter that enables the constitutive expression of intein.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image5.png 1x, https://static.igem.wiki/teams/5358/parts/image5.png 2x" src="https://static.igem.wiki/teams/5358/parts/image5.png"><!--]--></p><h3 id="pt7-luxi-t"><!--[-->PT7-LuxI-T<!--]--></h3><p><!--[-->Used to construct a regulatory circuit with T7 polymerase.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image6.png 1x, https://static.igem.wiki/teams/5358/parts/image6.png 2x" src="https://static.igem.wiki/teams/5358/parts/image6.png"><!--]--></p><h3 id="preca-hrpr-t-pj23100-luxr-pluxr-hrps-t-phrpl-bsfbfp-t"><!--[-->PrecA-hrpR-T- Pj23100-LuxR- PluxR-hrpS-T- PhrpL-BsFbFp-T<!--]--></h3><p><!--[-->PrecA responds to cellular oxidative stress damage and expresses hrpR. PluxR responds to the binding of LuxR and AHL, ultimately leading to the expression of hrpS. HrpS and hrpR together activate PhrpL, which expresses BsFbFp, resulting in fluorescence characterization in bacteria.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image7.png 1x, https://static.igem.wiki/teams/5358/parts/image7.png 2x" src="https://static.igem.wiki/teams/5358/parts/image7.png"><!--]--></p><h3 id="pj23100-n-t7rnap-er-lbd-intein-c-t7rnap-t-pt7-luxi-t"><!--[-->Pj23100-N-T7RNAP-ER-LBD intein-C-T7RNAP-T- PT7-LuxI-T<!--]--></h3><p><!--[-->PgapA constitutively initiates the expression of intein. When cells are affected by endocrine disruptors, intein is cleaved, allowing the N-terminal and C-terminal of T7 polymerase to come together and activate PT7 to express LuxI, which collaborates with other bacteria to initiate signaling expression.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image8.png 1x, https://static.igem.wiki/teams/5358/parts/image8.png 2x" src="https://static.igem.wiki/teams/5358/parts/image8.png"> The composite part is composed of 12 basic parts, which can realize the detection of oxidative stress damage level and signal amplification function of E.coli.<!--]--></p><p><!--[-->Our part successfully detected DNA damage and endocrine disruptors caused by the damaging reagent NA. Increasing the signal output while improving the sensitivity of the sensor, which broadens the application range of the sensor and is more suitable for the detection of new chemical reagents.<!--]--></p><h2 id="reference-parts"><!--[-->Reference Parts:<!--]--></h2><table><!--[--><thead><!--[--><tr><!--[--><th><!--[-->Part Name<!--]--></th><th><!--[-->Type<!--]--></th><th><!--[-->Description<!--]--></th><th><!--[-->Length<!--]--></th><!--]--></tr><!--]--></thead><tbody><!--[--><tr><!--[--><td><!--[-->BBa_K629001<!--]--></td><td><!--[-->Regulatory<!--]--></td><td><!--[-->This part could be started with exposure to irradiation, UV, nalidixic acid and activate SOS system to activate DNA repair.<!--]--></td><td><!--[-->124bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_K2967008<!--]--></td><td><!--[-->Coding<!--]--></td><td><!--[-->The activator protein HrpR in hrp gene regulatory network<!--]--></td><td><!--[-->948 bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_K2967009<!--]--></td><td><!--[-->Coding<!--]--></td><td><!--[-->The activator protein HrpS in hrp gene regulatory network<!--]--></td><td><!--[-->912pb<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_K2967011<!--]--></td><td><!--[-->Regulatory<!--]--></td><td><!--[-->The inducible promoter in hrp gene regulatory network<!--]--></td><td><!--[-->208 bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_B0015<!--]--></td><td><!--[-->Terminator<!--]--></td><td><!--[-->double terminator (B0010-B0012)<!--]--></td><td><!--[-->129bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_K081005<!--]--></td><td><!--[-->Regulatory<!--]--></td><td><!--[-->Constitutive promoters of gene circuits<!--]--></td><td><!--[-->58bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_J37019<!--]--></td><td><!--[-->Regulatory-Coding<!--]--></td><td><!--[-->The inducible promoter in Lux gene regulatory network andthe LuxI-mediated response proteins LuxR in hrp gene regulatory network<!--]--></td><td><!--[-->862bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_C0261<!--]--></td><td><!--[-->Coding<!--]--></td><td><!--[-->The activator protein LuxI in Lux gene regulatory network<!--]--></td><td><!--[-->661bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_E0840<!--]--></td><td><!--[-->Coding<!--]--></td><td><!--[-->Green fluorescent protein<!--]--></td><td><!--[-->878bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_I712074<!--]--></td><td><!--[-->Regulatory<!--]--></td><td><!--[-->The inducible promoter in T7 gene regulatory network<!--]--></td><td><!--[-->46 bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_B0030<!--]--></td><td><!--[-->RBS<!--]--></td><td><!--[-->Modified from R. Weiss<!--]--></td><td><!--[-->15bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_K2610040<!--]--></td><td><!--[-->Regulatory<!--]--></td><td><!--[-->Constitutive promoters of gene circuits<!--]--></td><td><!--[-->15bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_R0062<!--]--></td><td><!--[-->Regulatory<!--]--></td><td><!--[-->The inducible promoter in lux gene regulatory network<!--]--></td><td><!--[-->55bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_C0062<!--]--></td><td><!--[-->Coding<!--]--></td><td><!--[-->The activator protein luxR in lux gene regulatory network<!--]--></td><td><!--[-->781bp<!--]--></td><!--]--></tr><!--]--></tbody><!--]--></table></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a aria-current="page" href="/bit/parts" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/parts/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-jH31r7kexy":11},null,"/parts",1727868995304]</script>
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tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><div class="el-menu--vertical el-menu--popup-container"><ul class="el-menu el-menu--popup el-menu--popup-right-start" style="--el-menu-level:1;"><!--[--><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Members<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Attributions<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Contribution<!--]--><!--[--><!--]--><!--]--></li><!--]--></ul></div><!--]--><!--v-if--><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--><!--teleport start anchor--><!--[--><div style="z-index:2011;position:absolute;left:0;top:0;display:none;" class="el-popper is-pure is-light" tabindex="-1" aria-hidden="true"><!--[--><!--[--><!--[--><!--[--><div class="el-menu--vertical el-menu--popup-container"><ul class="el-menu el-menu--popup el-menu--popup-right-start" style="--el-menu-level:1;"><!--[--><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Education<!--]--><!--[--><!--]--><!--]--></li><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->Integrated HP<!--]--><!--[--><!--]--><!--]--></li><!--]--></ul></div><!--]--><!--v-if--><!--]--><!--]--><!--]--></div><!--]--><!--teleport anchor--></div><div id="__nuxt"><!--[--><div class="nuxt-loading-indicator" style="position:fixed;top:0;right:0;left:0;pointer-events:none;width:auto;height:3px;opacity:0;background:repeating-linear-gradient(to right,#00dc82 0%,#34cdfe 50%,#0047e1 100%);background-size:Infinity% auto;transform:scaleX(0%);transform-origin:left;transition:transform 0.1s, height 0.4s, opacity 0.4s;z-index:999999;"></div><div class="mx-10"><header class="fixed w-full"><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-desktop px-10 hidden lg:flex"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" 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start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Wet Lab<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Dry Lab<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Team<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Human Practices<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li></ul><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-mobile px-10 flex lg:hidden"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 2x" class="cursor-pointer py-2" src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div class="grow"></div><!--[--><span class="el-avatar el-avatar--icon el-avatar--circle self-center cursor-pointer el-tooltip__trigger el-tooltip__trigger" style=""><i class="el-icon" style=""><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M160 448a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zM160 896a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32z"></path></svg><!--]--></i></span><!--teleport start--><!--teleport end--><!--]--></ul></header><div class="pt-[8rem]"><!--[--><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="cover" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/wetlab.webp 1x, https://static.igem.wiki/teams/5358/banner/wetlab.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/wetlab.webp" data-v-1b4324c9><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg" data-v-1b4324c9>Parts</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10" data-v-1b4324c9><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[200px] md:mr-10" data-v-1b4324c9><h1 class="mt-0" data-v-1b4324c9>TOC</h1><ul data-v-1b4324c9><!--[--><li class="my-5" data-v-1b4324c9><a class="cursor-pointer" data-v-1b4324c9>Basic Parts</a></li><li class="my-5" data-v-1b4324c9><a class="cursor-pointer" data-v-1b4324c9>Composite Parts</a></li><li class="my-5" data-v-1b4324c9><a class="cursor-pointer" data-v-1b4324c9>Reference Parts:</a></li><!--]--></ul></aside><div class="content" data-v-1b4324c9><p><!--[-->Team BIT registered 2 basic parts and  8 new composite parts.<!--]--></p><p><!--[-->The sequence source and construction method of the composite part are demonstrated in the Experiments section and Results section. Please click on the Part Name to shift to the registry for viewing further information.<!--]--></p><h2 id="basic-parts"><!--[-->Basic Parts<!--]--></h2><table><!--[--><thead><!--[--><tr><!--[--><th><!--[-->Part Code<!--]--></th><th><!--[-->Part Name<!--]--></th><th><!--[-->Type<!--]--></th><!--]--></tr><!--]--></thead><tbody><!--[--><tr><!--[--><td><!--[-->BBa_K5358010<!--]--></td><td><!--[-->BsFbFp<!--]--></td><td><!--[-->Reporter<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_K5358011<!--]--></td><td><!--[-->(N)T7RNAP-ERintein-(C)T7RNAP<!--]--></td><td><!--[-->Coding<!--]--></td><!--]--></tr><!--]--></tbody><!--]--></table><h2 id="composite-parts"><!--[-->Composite Parts<!--]--></h2><h3 id="preca-hrpr-t"><!--[-->PrecA-hrpR-T<!--]--></h3><p><!--[-->When cells are subjected to oxidative damage, PrecA initiates the expression of the downstream hrpR gene.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image1.png 1x, https://static.igem.wiki/teams/5358/parts/image1.png 2x" src="https://static.igem.wiki/teams/5358/parts/image1.png"><!--]--></p><h3 id="pj23100-luxr-t"><!--[-->Pj23100-LuxR-T<!--]--></h3><p><!--[-->Pj23100 is a constitutive promoter that enables the constitutive expression of LuxR.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image2.png 1x, https://static.igem.wiki/teams/5358/parts/image2.png 2x" src="https://static.igem.wiki/teams/5358/parts/image2.png"><!--]--></p><h3 id="pluxr-hrps-t"><!--[-->PluxR-hrpS-T<!--]--></h3><p><!--[-->Used for the constitutive regulation circuit with LuxR.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image3.png 1x, https://static.igem.wiki/teams/5358/parts/image3.png 2x" src="https://static.igem.wiki/teams/5358/parts/image3.png"><!--]--></p><h3 id="phrpl-bsfbfp-t"><!--[-->PhrpL-BsFbFp-T<!--]--></h3><p><!--[-->Used for the constitutive regulation characterization project with hrpR.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image4.png 1x, https://static.igem.wiki/teams/5358/parts/image4.png 2x" src="https://static.igem.wiki/teams/5358/parts/image4.png"><!--]--></p><h3 id="pgapa-n-t7rnap-er-lbd-intein-c-t7rnap-t"><!--[-->PgapA-N-T7RNAP-ER-LBD intein-C-T7RNAP-T<!--]--></h3><p><!--[-->PgapA is a constitutive promoter that enables the constitutive expression of intein.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image5.png 1x, https://static.igem.wiki/teams/5358/parts/image5.png 2x" src="https://static.igem.wiki/teams/5358/parts/image5.png"><!--]--></p><h3 id="pt7-luxi-t"><!--[-->PT7-LuxI-T<!--]--></h3><p><!--[-->Used to construct a regulatory circuit with T7 polymerase.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image6.png 1x, https://static.igem.wiki/teams/5358/parts/image6.png 2x" src="https://static.igem.wiki/teams/5358/parts/image6.png"><!--]--></p><h3 id="preca-hrpr-t-pj23100-luxr-pluxr-hrps-t-phrpl-bsfbfp-t"><!--[-->PrecA-hrpR-T- Pj23100-LuxR- PluxR-hrpS-T- PhrpL-BsFbFp-T<!--]--></h3><p><!--[-->PrecA responds to cellular oxidative stress damage and expresses hrpR. PluxR responds to the binding of LuxR and AHL, ultimately leading to the expression of hrpS. HrpS and hrpR together activate PhrpL, which expresses BsFbFp, resulting in fluorescence characterization in bacteria.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image7.png 1x, https://static.igem.wiki/teams/5358/parts/image7.png 2x" src="https://static.igem.wiki/teams/5358/parts/image7.png"><!--]--></p><h3 id="pj23100-n-t7rnap-er-lbd-intein-c-t7rnap-t-pt7-luxi-t"><!--[-->Pj23100-N-T7RNAP-ER-LBD intein-C-T7RNAP-T- PT7-LuxI-T<!--]--></h3><p><!--[-->PgapA constitutively initiates the expression of intein. When cells are affected by endocrine disruptors, intein is cleaved, allowing the N-terminal and C-terminal of T7 polymerase to come together and activate PT7 to express LuxI, which collaborates with other bacteria to initiate signaling expression.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/parts/image8.png 1x, https://static.igem.wiki/teams/5358/parts/image8.png 2x" src="https://static.igem.wiki/teams/5358/parts/image8.png"> The composite part is composed of 12 basic parts, which can realize the detection of oxidative stress damage level and signal amplification function of E.coli.<!--]--></p><p><!--[-->Our part successfully detected DNA damage and endocrine disruptors caused by the damaging reagent NA. Increasing the signal output while improving the sensitivity of the sensor, which broadens the application range of the sensor and is more suitable for the detection of new chemical reagents.<!--]--></p><h2 id="reference-parts"><!--[-->Reference Parts:<!--]--></h2><table><!--[--><thead><!--[--><tr><!--[--><th><!--[-->Part Name<!--]--></th><th><!--[-->Type<!--]--></th><th><!--[-->Description<!--]--></th><th><!--[-->Length<!--]--></th><!--]--></tr><!--]--></thead><tbody><!--[--><tr><!--[--><td><!--[-->BBa_K629001<!--]--></td><td><!--[-->Regulatory<!--]--></td><td><!--[-->This part could be started with exposure to irradiation, UV, nalidixic acid and activate SOS system to activate DNA repair.<!--]--></td><td><!--[-->124bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_K2967008<!--]--></td><td><!--[-->Coding<!--]--></td><td><!--[-->The activator protein HrpR in hrp gene regulatory network<!--]--></td><td><!--[-->948 bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_K2967009<!--]--></td><td><!--[-->Coding<!--]--></td><td><!--[-->The activator protein HrpS in hrp gene regulatory network<!--]--></td><td><!--[-->912pb<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_K2967011<!--]--></td><td><!--[-->Regulatory<!--]--></td><td><!--[-->The inducible promoter in hrp gene regulatory network<!--]--></td><td><!--[-->208 bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_B0015<!--]--></td><td><!--[-->Terminator<!--]--></td><td><!--[-->double terminator (B0010-B0012)<!--]--></td><td><!--[-->129bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_K081005<!--]--></td><td><!--[-->Regulatory<!--]--></td><td><!--[-->Constitutive promoters of gene circuits<!--]--></td><td><!--[-->58bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_J37019<!--]--></td><td><!--[-->Regulatory-Coding<!--]--></td><td><!--[-->The inducible promoter in Lux gene regulatory network andthe LuxI-mediated response proteins LuxR in hrp gene regulatory network<!--]--></td><td><!--[-->862bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_C0261<!--]--></td><td><!--[-->Coding<!--]--></td><td><!--[-->The activator protein LuxI in Lux gene regulatory network<!--]--></td><td><!--[-->661bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_E0840<!--]--></td><td><!--[-->Coding<!--]--></td><td><!--[-->Green fluorescent protein<!--]--></td><td><!--[-->878bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_I712074<!--]--></td><td><!--[-->Regulatory<!--]--></td><td><!--[-->The inducible promoter in T7 gene regulatory network<!--]--></td><td><!--[-->46 bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_B0030<!--]--></td><td><!--[-->RBS<!--]--></td><td><!--[-->Modified from R. Weiss<!--]--></td><td><!--[-->15bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_K2610040<!--]--></td><td><!--[-->Regulatory<!--]--></td><td><!--[-->Constitutive promoters of gene circuits<!--]--></td><td><!--[-->15bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_R0062<!--]--></td><td><!--[-->Regulatory<!--]--></td><td><!--[-->The inducible promoter in lux gene regulatory network<!--]--></td><td><!--[-->55bp<!--]--></td><!--]--></tr><tr><!--[--><td><!--[-->BBa_C0062<!--]--></td><td><!--[-->Coding<!--]--></td><td><!--[-->The activator protein luxR in lux gene regulatory network<!--]--></td><td><!--[-->781bp<!--]--></td><!--]--></tr><!--]--></tbody><!--]--></table></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a aria-current="page" href="/bit/parts" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/parts/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-jH31r7kexy":11},null,"/parts",1727871092344]</script>
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diff --git a/.output/public/result/_payload.json b/.output/public/result/_payload.json
index 3272003b8324f7023b86ce0481d63add7e06334c..b7dcc5ba0894d1ad87e2ef87a4698b3163d87628 100644
--- a/.output/public/result/_payload.json
+++ b/.output/public/result/_payload.json
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-[{"data":1,"prerenderedAt":309},["Reactive",2],{"content-query-WfQ6VD3q4y":3},{"_path":4,"_dir":5,"_draft":6,"_partial":6,"_locale":5,"title":7,"description":8,"body":9,"_type":303,"_id":304,"_source":305,"_file":306,"_stem":307,"_extension":308},"/wetlab-results","",false,"Results","Endocrine disruptors are a class of chemicals that can interfere with the normal function of the endocrine system, and they may mimic or inhibit the effects of natural hormones, thereby affecting the physiological processes of the body. These substances are widely found in plastics, pesticides, industrial chemicals, and daily consumer goods. They enter the human body through the food chain or through direct contact, interfering with hormonal balance, and may cause reproductive health problems, developmental disorders, metabolic disorders and even some types of cancer.",{"type":10,"children":11,"toc":291},"root",[12,19,24,31,38,43,48,56,63,74,80,85,92,99,108,113,120,127,136,141,148,157,163,168,175,182,191,197,202,209,216,225,231,238,243,250,259,266,275,282],{"type":13,"tag":14,"props":15,"children":16},"element","p",{},[17],{"type":18,"value":8},"text",{"type":13,"tag":14,"props":20,"children":21},{},[22],{"type":18,"value":23},"When endocrine disruptors affect hormone activity, they may also induce cellular oxidative stress because they increase the generation of reactive oxygen species (ROS), highly reactive chemicals that damage cellular structures, including proteins, lipids, and DNA. Long-term oxidative stress accelerates cellular aging and may also lead to male infertility and the development of multiple chronic diseases. Thus, there is a close link between exposure to endocrine disruptors and cellular oxidative damage, a potential promoter to multiple health problems. Therefore, it is necessary to detect the endocrine interference effects of endocrine disruptors and oxidative damage effects in the relevant location samples.",{"type":13,"tag":25,"props":26,"children":28},"h2",{"id":27},"_1-puc19-preca-bsfbfp-bl21",[29],{"type":18,"value":30},"1. pUC19-PrecA-BsFbFp-BL21",{"type":13,"tag":32,"props":33,"children":35},"h3",{"id":34},"strain-construction",[36],{"type":18,"value":37},"Strain construction",{"type":13,"tag":14,"props":39,"children":40},{},[41],{"type":18,"value":42},"We plan to design a portable microbial sensor, in which the sensor chamber will be maintained in a nearly anoxic state. Under anoxic conditions, the fluorescence intensity of EGFP diminishes, and its detection limit decreases; therefore, traditional EGFP is not the optimal choice for our application. We have constructed the PrecA-BsFbFP circuit, where BsFbFP is an anoxic fluorescent protein that exhibits fluorescence intensity nearly equivalent to that of EGFP under anoxic conditions, while significantly improving the detection limit. Consequently, we will utilize BsFbFP as the expression product of our engineered strain for detection characterization.",{"type":13,"tag":14,"props":44,"children":45},{},[46],{"type":18,"value":47},"We obtained the PrecA-BsFbFp circuit and the recA-eGFP (hereafter referred to as wild) circuit through PCR.The gene circuits were ligated to the pUC19 vector using enzyme ligation and transformed into BL21 competent cells . The transformed cells were spread onto LB solid medium supplemented with ampicillin. Single colonies that grew were selected for amplification and sent to Jinweizhi (Suzhou) Biochemistry Co. for sequencing, and the remaining bacteria were preserved in glycerol.",{"type":13,"tag":14,"props":49,"children":50},{},[51],{"type":13,"tag":52,"props":53,"children":55},"img",{"alt":52,"src":54},"https://static.igem.wiki/teams/5358/experiment/fig1.png",[],{"type":13,"tag":14,"props":57,"children":58},{},[59],{"type":13,"tag":52,"props":60,"children":62},{"alt":52,"src":61},"https://static.igem.wiki/teams/5358/experiment/bsfbfp.png",[],{"type":13,"tag":14,"props":64,"children":65},{},[66],{"type":13,"tag":67,"props":68,"children":71},"span",{"className":69},[70],"fig-title",[72],{"type":18,"value":73},"Figure 1. Results of the enzyme digestion verification experiment for pUC19-PrecA-BsFbFP-BL21.",{"type":13,"tag":32,"props":75,"children":77},{"id":76},"characterization-of-experiments",[78],{"type":18,"value":79},"Characterization of experiments",{"type":13,"tag":14,"props":81,"children":82},{},[83],{"type":18,"value":84},"Firstly, we performed screening of the pUC19-PrecA-BsFbFp-BL21. The transformed wild-type bacteria were cultured on a shaker until the OD value reached 0.2, followed by the addition of NA for induction for 2 h. In aerobic conditions, BsFbFp is significantly less characterized than EGFP, while the RFU is close, and BsFbFp has a high detection limit, indicating that BsFbFp is an excellent choice for characterization in anaerobic conditions.",{"type":13,"tag":14,"props":86,"children":87},{},[88],{"type":13,"tag":52,"props":89,"children":91},{"alt":52,"src":90},"https://static.igem.wiki/teams/5358/experiment/o2-fluo.png",[],{"type":13,"tag":14,"props":93,"children":94},{},[95],{"type":13,"tag":52,"props":96,"children":98},{"alt":52,"src":97},"https://static.igem.wiki/teams/5358/experiment/o2-rfu.png",[],{"type":13,"tag":14,"props":100,"children":101},{},[102],{"type":13,"tag":67,"props":103,"children":105},{"className":104},[70],[106],{"type":18,"value":107},"Figure 2. Characterization results of BsFbFP and EGFP under anoxic and NA gradient injury conditions.",{"type":13,"tag":14,"props":109,"children":110},{},[111],{"type":18,"value":112},"We have characterized the pUC19-PrecA-BsFbFp-BL21 under anaerobic conditions.The transformed wild-type bacteria were cultured on a shaker until the OD value reached 0.2, followed by the addition of NA for induction for 2 h.As the concentration of NA increased, the fluorescence intensity of engineering strains increased accordingly. Several experiments proved that the RFU value increased with the concentration of NA in the anaerobic environment.",{"type":13,"tag":14,"props":114,"children":115},{},[116],{"type":13,"tag":52,"props":117,"children":119},{"alt":52,"src":118},"https://static.igem.wiki/teams/5358/experiment/10-rfu.png",[],{"type":13,"tag":14,"props":121,"children":122},{},[123],{"type":13,"tag":52,"props":124,"children":126},{"alt":52,"src":125},"https://static.igem.wiki/teams/5358/experiment/10-rfu-more.png",[],{"type":13,"tag":14,"props":128,"children":129},{},[130],{"type":13,"tag":67,"props":131,"children":133},{"className":132},[70],[134],{"type":18,"value":135},"Figure 3. Fluorescence intensity characterization of BsFbFP under NA gradient injury conditions in 10-lianpao chip cultures, expressed as RFU.",{"type":13,"tag":14,"props":137,"children":138},{},[139],{"type":18,"value":140},"Later, we compared the characterization difference between DH5α strain and BL21 strain, and showed that the BL21 strain has higher sensitivity to injury, reflecting its advantage as a representative strain.",{"type":13,"tag":14,"props":142,"children":143},{},[144],{"type":13,"tag":52,"props":145,"children":147},{"alt":52,"src":146},"https://static.igem.wiki/teams/5358/experiment/o2-fluo-more.png",[],{"type":13,"tag":14,"props":149,"children":150},{},[151],{"type":13,"tag":67,"props":152,"children":154},{"className":153},[70],[155],{"type":18,"value":156},"Figure 4. Fluorescence characterization gradients of DH5α and BL21 under aerobic and NA gradient injury conditions.",{"type":13,"tag":25,"props":158,"children":160},{"id":159},"_2-pet28-preca-hrpr-hrps-phrpl-bsfbfp-bl21",[161],{"type":18,"value":162},"2. pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21",{"type":13,"tag":14,"props":164,"children":165},{},[166],{"type":18,"value":167},"The gene circuits were ligated to the pET28 vector using enzyme ligation and transformed into BL21 competent cells . The transformed cells were spread onto LB solid medium supplemented with ampicillin.Preserved strains were stored in glycerol.",{"type":13,"tag":14,"props":169,"children":170},{},[171],{"type":13,"tag":52,"props":172,"children":174},{"alt":52,"src":173},"https://static.igem.wiki/teams/5358/experiment/hrp.png",[],{"type":13,"tag":14,"props":176,"children":177},{},[178],{"type":13,"tag":52,"props":179,"children":181},{"alt":52,"src":180},"https://static.igem.wiki/teams/5358/experiment/2-enzyme-digestion.png",[],{"type":13,"tag":14,"props":183,"children":184},{},[185],{"type":13,"tag":67,"props":186,"children":188},{"className":187},[70],[189],{"type":18,"value":190},"Figure 5. Results of the enzyme digestion verification experiment for pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21.",{"type":13,"tag":25,"props":192,"children":194},{"id":193},"_3-puc19-pbad-n-t7rnap-er-lbd-intein-c-t7rnap-bsfbfp",[195],{"type":18,"value":196},"3. pUC19-PBAD-N-T7RNAP-ER-LBD intein-C-T7RNAP-BsFbFp",{"type":13,"tag":14,"props":198,"children":199},{},[200],{"type":18,"value":201},"The gene sequencing conducted by our company has successfully validated the synthesis of the intein of the biological circuit.",{"type":13,"tag":14,"props":203,"children":204},{},[205],{"type":13,"tag":52,"props":206,"children":208},{"alt":52,"src":207},"https://static.igem.wiki/teams/5358/experiment/fig3.png",[],{"type":13,"tag":14,"props":210,"children":211},{},[212],{"type":13,"tag":52,"props":213,"children":215},{"alt":52,"src":214},"https://static.igem.wiki/teams/5358/experiment/gene-squencing.png",[],{"type":13,"tag":14,"props":217,"children":218},{},[219],{"type":13,"tag":67,"props":220,"children":222},{"className":221},[70],[223],{"type":18,"value":224},"Figure 6. Gene sequencing results of the ER-LBD intein.",{"type":13,"tag":25,"props":226,"children":228},{"id":227},"_4-psb1c3-pj23100-luxi-bl21-and-psb1c3-plux-luxr-egfp-bl21",[229],{"type":18,"value":230},"4. psB1C3-Pj23100-LuxI-BL21 and psB1C3-Plux-LuxR-EGFP-BL21",{"type":13,"tag":14,"props":232,"children":233},{},[234],{"type":13,"tag":52,"props":235,"children":237},{"alt":52,"src":236},"https://static.igem.wiki/teams/5358/experiment/fig4.png",[],{"type":13,"tag":14,"props":239,"children":240},{},[241],{"type":18,"value":242},"We obtained by PCR the Pj233100, Plux-LuxR, LuxI, and EGFP and ligated it to the psB1C3 plasmid into E. coli BL21. The transformed cells were spread onto LB solid medium supplemented with chloramphenicol. Recombinants were used for characterization experiments and stored in glycerol.",{"type":13,"tag":14,"props":244,"children":245},{},[246],{"type":13,"tag":52,"props":247,"children":249},{"alt":52,"src":248},"https://static.igem.wiki/teams/5358/experiment/pcr.png",[],{"type":13,"tag":14,"props":251,"children":252},{},[253],{"type":13,"tag":67,"props":254,"children":256},{"className":255},[70],[257],{"type":18,"value":258},"Figure 7. PCR products of the Pj23100-LuxI fragment and the Plux-LuxR-EGFP fragment.",{"type":13,"tag":14,"props":260,"children":261},{},[262],{"type":13,"tag":52,"props":263,"children":265},{"alt":52,"src":264},"https://static.igem.wiki/teams/5358/experiment/lux-digestion.png",[],{"type":13,"tag":14,"props":267,"children":268},{},[269],{"type":13,"tag":67,"props":270,"children":272},{"className":271},[70],[273],{"type":18,"value":274},"Figure 8. Enzyme digestion verification of psB1C3-Pj23100-LuxI-BL21 and Plux-LuxR-EGFP-BL21.",{"type":13,"tag":14,"props":276,"children":277},{},[278],{"type":13,"tag":52,"props":279,"children":281},{"alt":52,"src":280},"https://static.igem.wiki/teams/5358/experiment/ligation.png",[],{"type":13,"tag":14,"props":283,"children":284},{},[285],{"type":13,"tag":67,"props":286,"children":288},{"className":287},[70],[289],{"type":18,"value":290},"Figure 9. Enzyme Ligation Product of psB1C3-Pj23100-LuxI-BL21 and Plux-LuxR-EGFP-BL21.",{"title":5,"searchDepth":292,"depth":293,"links":294},1,3,[295,300,301,302],{"id":27,"depth":296,"text":30,"children":297},2,[298,299],{"id":34,"depth":293,"text":37},{"id":76,"depth":293,"text":79},{"id":159,"depth":296,"text":162},{"id":193,"depth":296,"text":196},{"id":227,"depth":296,"text":230},"markdown","content:wetlab-results.md","content","wetlab-results.md","wetlab-results","md",1727868995288]
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They enter the human body through the food chain or through direct contact, interfering with hormonal balance, and may cause reproductive health problems, developmental disorders, metabolic disorders and even some types of cancer.",{"type":10,"children":11,"toc":291},"root",[12,19,24,31,38,43,48,56,63,74,80,85,92,99,108,113,120,127,136,141,148,157,163,168,175,182,191,197,202,209,216,225,231,238,243,250,259,266,275,282],{"type":13,"tag":14,"props":15,"children":16},"element","p",{},[17],{"type":18,"value":8},"text",{"type":13,"tag":14,"props":20,"children":21},{},[22],{"type":18,"value":23},"When endocrine disruptors affect hormone activity, they may also induce cellular oxidative stress because they increase the generation of reactive oxygen species (ROS), highly reactive chemicals that damage cellular structures, including proteins, lipids, and DNA. Long-term oxidative stress accelerates cellular aging and may also lead to male infertility and the development of multiple chronic diseases. Thus, there is a close link between exposure to endocrine disruptors and cellular oxidative damage, a potential promoter to multiple health problems. Therefore, it is necessary to detect the endocrine interference effects of endocrine disruptors and oxidative damage effects in the relevant location samples.",{"type":13,"tag":25,"props":26,"children":28},"h2",{"id":27},"_1-puc19-preca-bsfbfp-bl21",[29],{"type":18,"value":30},"1. pUC19-PrecA-BsFbFp-BL21",{"type":13,"tag":32,"props":33,"children":35},"h3",{"id":34},"strain-construction",[36],{"type":18,"value":37},"Strain construction",{"type":13,"tag":14,"props":39,"children":40},{},[41],{"type":18,"value":42},"We plan to design a portable microbial sensor, in which the sensor chamber will be maintained in a nearly anoxic state. Under anoxic conditions, the fluorescence intensity of EGFP diminishes, and its detection limit decreases; therefore, traditional EGFP is not the optimal choice for our application. We have constructed the PrecA-BsFbFP circuit, where BsFbFP is an anoxic fluorescent protein that exhibits fluorescence intensity nearly equivalent to that of EGFP under anoxic conditions, while significantly improving the detection limit. Consequently, we will utilize BsFbFP as the expression product of our engineered strain for detection characterization.",{"type":13,"tag":14,"props":44,"children":45},{},[46],{"type":18,"value":47},"We obtained the PrecA-BsFbFp circuit and the recA-eGFP (hereafter referred to as wild) circuit through PCR.The gene circuits were ligated to the pUC19 vector using enzyme ligation and transformed into BL21 competent cells . The transformed cells were spread onto LB solid medium supplemented with ampicillin. Single colonies that grew were selected for amplification and sent to Jinweizhi (Suzhou) Biochemistry Co. for sequencing, and the remaining bacteria were preserved in glycerol.",{"type":13,"tag":14,"props":49,"children":50},{},[51],{"type":13,"tag":52,"props":53,"children":55},"img",{"alt":52,"src":54},"https://static.igem.wiki/teams/5358/experiment/fig1.png",[],{"type":13,"tag":14,"props":57,"children":58},{},[59],{"type":13,"tag":52,"props":60,"children":62},{"alt":52,"src":61},"https://static.igem.wiki/teams/5358/experiment/bsfbfp.png",[],{"type":13,"tag":14,"props":64,"children":65},{},[66],{"type":13,"tag":67,"props":68,"children":71},"span",{"className":69},[70],"fig-title",[72],{"type":18,"value":73},"Figure 1. Results of the enzyme digestion verification experiment for pUC19-PrecA-BsFbFP-BL21.",{"type":13,"tag":32,"props":75,"children":77},{"id":76},"characterization-of-experiments",[78],{"type":18,"value":79},"Characterization of experiments",{"type":13,"tag":14,"props":81,"children":82},{},[83],{"type":18,"value":84},"Firstly, we performed screening of the pUC19-PrecA-BsFbFp-BL21. The transformed wild-type bacteria were cultured on a shaker until the OD value reached 0.2, followed by the addition of NA for induction for 2 h. In aerobic conditions, BsFbFp is significantly less characterized than EGFP, while the RFU is close, and BsFbFp has a high detection limit, indicating that BsFbFp is an excellent choice for characterization in anaerobic conditions.",{"type":13,"tag":14,"props":86,"children":87},{},[88],{"type":13,"tag":52,"props":89,"children":91},{"alt":52,"src":90},"https://static.igem.wiki/teams/5358/experiment/o2-fluo.png",[],{"type":13,"tag":14,"props":93,"children":94},{},[95],{"type":13,"tag":52,"props":96,"children":98},{"alt":52,"src":97},"https://static.igem.wiki/teams/5358/experiment/o2-rfu.png",[],{"type":13,"tag":14,"props":100,"children":101},{},[102],{"type":13,"tag":67,"props":103,"children":105},{"className":104},[70],[106],{"type":18,"value":107},"Figure 2. Characterization results of BsFbFP and EGFP under anoxic and NA gradient injury conditions.",{"type":13,"tag":14,"props":109,"children":110},{},[111],{"type":18,"value":112},"We have characterized the pUC19-PrecA-BsFbFp-BL21 under anaerobic conditions.The transformed wild-type bacteria were cultured on a shaker until the OD value reached 0.2, followed by the addition of NA for induction for 2 h.As the concentration of NA increased, the fluorescence intensity of engineering strains increased accordingly. Several experiments proved that the RFU value increased with the concentration of NA in the anaerobic environment.",{"type":13,"tag":14,"props":114,"children":115},{},[116],{"type":13,"tag":52,"props":117,"children":119},{"alt":52,"src":118},"https://static.igem.wiki/teams/5358/experiment/10-rfu.png",[],{"type":13,"tag":14,"props":121,"children":122},{},[123],{"type":13,"tag":52,"props":124,"children":126},{"alt":52,"src":125},"https://static.igem.wiki/teams/5358/experiment/10-rfu-more.png",[],{"type":13,"tag":14,"props":128,"children":129},{},[130],{"type":13,"tag":67,"props":131,"children":133},{"className":132},[70],[134],{"type":18,"value":135},"Figure 3. Fluorescence intensity characterization of BsFbFP under NA gradient injury conditions in 10-lianpao chip cultures, expressed as RFU.",{"type":13,"tag":14,"props":137,"children":138},{},[139],{"type":18,"value":140},"Later, we compared the characterization difference between DH5α strain and BL21 strain, and showed that the BL21 strain has higher sensitivity to injury, reflecting its advantage as a representative strain.",{"type":13,"tag":14,"props":142,"children":143},{},[144],{"type":13,"tag":52,"props":145,"children":147},{"alt":52,"src":146},"https://static.igem.wiki/teams/5358/experiment/o2-fluo-more.png",[],{"type":13,"tag":14,"props":149,"children":150},{},[151],{"type":13,"tag":67,"props":152,"children":154},{"className":153},[70],[155],{"type":18,"value":156},"Figure 4. Fluorescence characterization gradients of DH5α and BL21 under aerobic and NA gradient injury conditions.",{"type":13,"tag":25,"props":158,"children":160},{"id":159},"_2-pet28-preca-hrpr-hrps-phrpl-bsfbfp-bl21",[161],{"type":18,"value":162},"2. pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21",{"type":13,"tag":14,"props":164,"children":165},{},[166],{"type":18,"value":167},"The gene circuits were ligated to the pET28 vector using enzyme ligation and transformed into BL21 competent cells . The transformed cells were spread onto LB solid medium supplemented with ampicillin.Preserved strains were stored in glycerol.",{"type":13,"tag":14,"props":169,"children":170},{},[171],{"type":13,"tag":52,"props":172,"children":174},{"alt":52,"src":173},"https://static.igem.wiki/teams/5358/experiment/hrp.png",[],{"type":13,"tag":14,"props":176,"children":177},{},[178],{"type":13,"tag":52,"props":179,"children":181},{"alt":52,"src":180},"https://static.igem.wiki/teams/5358/experiment/2-enzyme-digestion.png",[],{"type":13,"tag":14,"props":183,"children":184},{},[185],{"type":13,"tag":67,"props":186,"children":188},{"className":187},[70],[189],{"type":18,"value":190},"Figure 5. Results of the enzyme digestion verification experiment for pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21.",{"type":13,"tag":25,"props":192,"children":194},{"id":193},"_3-puc19-pbad-n-t7rnap-er-lbd-intein-c-t7rnap-bsfbfp",[195],{"type":18,"value":196},"3. pUC19-PBAD-N-T7RNAP-ER-LBD intein-C-T7RNAP-BsFbFp",{"type":13,"tag":14,"props":198,"children":199},{},[200],{"type":18,"value":201},"The gene sequencing conducted by our company has successfully validated the synthesis of the intein of the biological circuit.",{"type":13,"tag":14,"props":203,"children":204},{},[205],{"type":13,"tag":52,"props":206,"children":208},{"alt":52,"src":207},"https://static.igem.wiki/teams/5358/experiment/fig3.png",[],{"type":13,"tag":14,"props":210,"children":211},{},[212],{"type":13,"tag":52,"props":213,"children":215},{"alt":52,"src":214},"https://static.igem.wiki/teams/5358/experiment/gene-squencing.png",[],{"type":13,"tag":14,"props":217,"children":218},{},[219],{"type":13,"tag":67,"props":220,"children":222},{"className":221},[70],[223],{"type":18,"value":224},"Figure 6. Gene sequencing results of the ER-LBD intein.",{"type":13,"tag":25,"props":226,"children":228},{"id":227},"_4-psb1c3-pj23100-luxi-bl21-and-psb1c3-plux-luxr-egfp-bl21",[229],{"type":18,"value":230},"4. psB1C3-Pj23100-LuxI-BL21 and psB1C3-Plux-LuxR-EGFP-BL21",{"type":13,"tag":14,"props":232,"children":233},{},[234],{"type":13,"tag":52,"props":235,"children":237},{"alt":52,"src":236},"https://static.igem.wiki/teams/5358/experiment/fig4.png",[],{"type":13,"tag":14,"props":239,"children":240},{},[241],{"type":18,"value":242},"We obtained by PCR the Pj233100, Plux-LuxR, LuxI, and EGFP and ligated it to the psB1C3 plasmid into E. coli BL21. The transformed cells were spread onto LB solid medium supplemented with chloramphenicol. Recombinants were used for characterization experiments and stored in glycerol.",{"type":13,"tag":14,"props":244,"children":245},{},[246],{"type":13,"tag":52,"props":247,"children":249},{"alt":52,"src":248},"https://static.igem.wiki/teams/5358/experiment/pcr.png",[],{"type":13,"tag":14,"props":251,"children":252},{},[253],{"type":13,"tag":67,"props":254,"children":256},{"className":255},[70],[257],{"type":18,"value":258},"Figure 7. PCR products of the Pj23100-LuxI fragment and the Plux-LuxR-EGFP fragment.",{"type":13,"tag":14,"props":260,"children":261},{},[262],{"type":13,"tag":52,"props":263,"children":265},{"alt":52,"src":264},"https://static.igem.wiki/teams/5358/experiment/lux-digestion.png",[],{"type":13,"tag":14,"props":267,"children":268},{},[269],{"type":13,"tag":67,"props":270,"children":272},{"className":271},[70],[273],{"type":18,"value":274},"Figure 8. Enzyme digestion verification of psB1C3-Pj23100-LuxI-BL21 and Plux-LuxR-EGFP-BL21.",{"type":13,"tag":14,"props":276,"children":277},{},[278],{"type":13,"tag":52,"props":279,"children":281},{"alt":52,"src":280},"https://static.igem.wiki/teams/5358/experiment/ligation.png",[],{"type":13,"tag":14,"props":283,"children":284},{},[285],{"type":13,"tag":67,"props":286,"children":288},{"className":287},[70],[289],{"type":18,"value":290},"Figure 9. Enzyme Ligation Product of psB1C3-Pj23100-LuxI-BL21 and Plux-LuxR-EGFP-BL21.",{"title":5,"searchDepth":292,"depth":293,"links":294},1,3,[295,300,301,302],{"id":27,"depth":296,"text":30,"children":297},2,[298,299],{"id":34,"depth":293,"text":37},{"id":76,"depth":293,"text":79},{"id":159,"depth":296,"text":162},{"id":193,"depth":296,"text":196},{"id":227,"depth":296,"text":230},"markdown","content:wetlab-results.md","content","wetlab-results.md","wetlab-results","md",1727871092328]
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.el-alert__description{color:var(--el-color-info)}.el-alert--info.is-dark{background-color:var(--el-color-info);color:var(--el-color-white)}.el-alert--warning{--el-alert-bg-color:var(--el-color-warning-light-9)}.el-alert--warning.is-light{background-color:var(--el-alert-bg-color)}.el-alert--warning.is-light,.el-alert--warning.is-light .el-alert__description{color:var(--el-color-warning)}.el-alert--warning.is-dark{background-color:var(--el-color-warning);color:var(--el-color-white)}.el-alert--error{--el-alert-bg-color:var(--el-color-error-light-9)}.el-alert--error.is-light{background-color:var(--el-alert-bg-color)}.el-alert--error.is-light,.el-alert--error.is-light .el-alert__description{color:var(--el-color-error)}.el-alert--error.is-dark{background-color:var(--el-color-error);color:var(--el-color-white)}.el-alert__content{display:flex;flex-direction:column;gap:4px}.el-alert .el-alert__icon{font-size:var(--el-alert-icon-size);margin-right:8px;width:var(--el-alert-icon-size)}.el-alert .el-alert__icon.is-big{font-size:var(--el-alert-icon-large-size);margin-right:12px;width:var(--el-alert-icon-large-size)}.el-alert__title{font-size:var(--el-alert-title-font-size);line-height:24px}.el-alert__title.with-description{font-size:var(--el-alert-title-with-description-font-size)}.el-alert .el-alert__description{font-size:var(--el-alert-description-font-size);margin:0}.el-alert .el-alert__close-btn{cursor:pointer;font-size:var(--el-alert-close-font-size);opacity:1;position:absolute;right:16px;top:12px}.el-alert .el-alert__close-btn.is-customed{font-size:var(--el-alert-close-customed-font-size);font-style:normal;line-height:24px;top:8px}.el-alert-fade-enter-from,.el-alert-fade-leave-active{opacity:0}.el-aside{box-sizing:border-box;flex-shrink:0;overflow:auto;width:var(--el-aside-width,300px)}.el-autocomplete{--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;display:inline-block;position:relative;width:var(--el-input-width)}.el-autocomplete__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-autocomplete__popper.el-popper,.el-autocomplete__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-border-color-light)}.el-autocomplete__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-autocomplete__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-autocomplete__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-autocomplete__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-autocomplete-suggestion{border-radius:var(--el-border-radius-base);box-sizing:border-box}.el-autocomplete-suggestion__wrap{box-sizing:border-box;max-height:280px;padding:10px 0}.el-autocomplete-suggestion__list{margin:0;padding:0}.el-autocomplete-suggestion li{color:var(--el-text-color-regular);cursor:pointer;font-size:var(--el-font-size-base);line-height:34px;list-style:none;margin:0;overflow:hidden;padding:0 20px;text-align:left;text-overflow:ellipsis;white-space:nowrap}.el-autocomplete-suggestion li.highlighted,.el-autocomplete-suggestion li:hover{background-color:var(--el-fill-color-light)}.el-autocomplete-suggestion li.divider{border-top:1px solid var(--el-color-black);margin-top:6px}.el-autocomplete-suggestion li.divider:last-child{margin-bottom:-6px}.el-autocomplete-suggestion.is-loading li{color:var(--el-text-color-secondary);font-size:20px;height:100px;line-height:100px;text-align:center}.el-autocomplete-suggestion.is-loading li:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-autocomplete-suggestion.is-loading li:hover{background-color:var(--el-bg-color-overlay)}.el-autocomplete-suggestion.is-loading .el-icon-loading{vertical-align:middle}.el-avatar{--el-avatar-text-color:var(--el-color-white);--el-avatar-bg-color:var(--el-text-color-disabled);--el-avatar-text-size:14px;--el-avatar-icon-size:18px;--el-avatar-border-radius:var(--el-border-radius-base);--el-avatar-size-large:56px;--el-avatar-size-small:24px;--el-avatar-size:40px;align-items:center;background:var(--el-avatar-bg-color);box-sizing:border-box;color:var(--el-avatar-text-color);display:inline-flex;font-size:var(--el-avatar-text-size);height:var(--el-avatar-size);justify-content:center;outline:none;overflow:hidden;text-align:center;width:var(--el-avatar-size)}.el-avatar>img{display:block;height:100%;width:100%}.el-avatar--circle{border-radius:50%}.el-avatar--square{border-radius:var(--el-avatar-border-radius)}.el-avatar--icon{font-size:var(--el-avatar-icon-size)}.el-avatar--small{--el-avatar-size:24px}.el-avatar--large{--el-avatar-size:56px}.el-backtop{--el-backtop-bg-color:var(--el-bg-color-overlay);--el-backtop-text-color:var(--el-color-primary);--el-backtop-hover-bg-color:var(--el-border-color-extra-light);align-items:center;background-color:var(--el-backtop-bg-color);border-radius:50%;box-shadow:var(--el-box-shadow-lighter);color:var(--el-backtop-text-color);cursor:pointer;display:flex;font-size:20px;height:40px;justify-content:center;position:fixed;width:40px;z-index:5}.el-backtop:hover{background-color:var(--el-backtop-hover-bg-color)}.el-backtop__icon{font-size:20px}.el-badge{--el-badge-bg-color:var(--el-color-danger);--el-badge-radius:10px;--el-badge-font-size:12px;--el-badge-padding:6px;--el-badge-size:18px;display:inline-block;position:relative;vertical-align:middle;width:-moz-fit-content;width:fit-content}.el-badge__content{align-items:center;background-color:var(--el-badge-bg-color);border:1px solid var(--el-bg-color);border-radius:var(--el-badge-radius);color:var(--el-color-white);display:inline-flex;font-size:var(--el-badge-font-size);height:var(--el-badge-size);justify-content:center;padding:0 var(--el-badge-padding);white-space:nowrap}.el-badge__content.is-fixed{position:absolute;right:calc(1px + var(--el-badge-size)/2);top:0;transform:translateY(-50%) translateX(100%);z-index:var(--el-index-normal)}.el-badge__content.is-fixed.is-dot{right:5px}.el-badge__content.is-dot{border-radius:50%;height:8px;padding:0;right:0;width:8px}.el-badge__content--primary{background-color:var(--el-color-primary)}.el-badge__content--success{background-color:var(--el-color-success)}.el-badge__content--warning{background-color:var(--el-color-warning)}.el-badge__content--info{background-color:var(--el-color-info)}.el-badge__content--danger{background-color:var(--el-color-danger)}.el-breadcrumb{font-size:14px;line-height:1}.el-breadcrumb:after,.el-breadcrumb:before{content:"";display:table}.el-breadcrumb:after{clear:both}.el-breadcrumb__separator{color:var(--el-text-color-placeholder);font-weight:700;margin:0 9px}.el-breadcrumb__separator.el-icon{font-weight:400;margin:0 6px}.el-breadcrumb__separator.el-icon svg{vertical-align:middle}.el-breadcrumb__item{align-items:center;display:inline-flex;float:left}.el-breadcrumb__inner{color:var(--el-text-color-regular)}.el-breadcrumb__inner a,.el-breadcrumb__inner.is-link{color:var(--el-text-color-primary);font-weight:700;text-decoration:none;transition:var(--el-transition-color)}.el-breadcrumb__inner a:hover,.el-breadcrumb__inner.is-link:hover{color:var(--el-color-primary);cursor:pointer}.el-breadcrumb__item:last-child .el-breadcrumb__inner,.el-breadcrumb__item:last-child .el-breadcrumb__inner a,.el-breadcrumb__item:last-child .el-breadcrumb__inner a:hover,.el-breadcrumb__item:last-child .el-breadcrumb__inner:hover{color:var(--el-text-color-regular);cursor:text;font-weight:400}.el-breadcrumb__item:last-child .el-breadcrumb__separator{display:none}.el-button-group{display:inline-block;vertical-align:middle}.el-button-group:after,.el-button-group:before{content:"";display:table}.el-button-group:after{clear:both}.el-button-group>.el-button{float:left;position:relative}.el-button-group>.el-button+.el-button{margin-left:0}.el-button-group>.el-button:first-child{border-bottom-right-radius:0;border-top-right-radius:0}.el-button-group>.el-button:last-child{border-bottom-left-radius:0;border-top-left-radius:0}.el-button-group>.el-button:first-child:last-child{border-bottom-left-radius:var(--el-border-radius-base);border-bottom-right-radius:var(--el-border-radius-base);border-top-left-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-button-group>.el-button:first-child:last-child.is-round{border-radius:var(--el-border-radius-round)}.el-button-group>.el-button:first-child:last-child.is-circle{border-radius:50%}.el-button-group>.el-button:not(:first-child):not(:last-child){border-radius:0}.el-button-group>.el-button:not(:last-child){margin-right:-1px}.el-button-group>.el-button.is-active,.el-button-group>.el-button:active,.el-button-group>.el-button:focus,.el-button-group>.el-button:hover{z-index:1}.el-button-group>.el-dropdown>.el-button{border-bottom-left-radius:0;border-left-color:var(--el-button-divide-border-color);border-top-left-radius:0}.el-button-group .el-button--primary:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--primary:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--primary:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--success:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--success:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--success:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--warning:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--warning:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--warning:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--danger:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--danger:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--danger:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--info:first-child{border-right-color:var(--el-button-divide-border-color)}.el-button-group .el-button--info:last-child{border-left-color:var(--el-button-divide-border-color)}.el-button-group .el-button--info:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button{--el-button-font-weight:var(--el-font-weight-primary);--el-button-border-color:var(--el-border-color);--el-button-bg-color:var(--el-fill-color-blank);--el-button-text-color:var(--el-text-color-regular);--el-button-disabled-text-color:var(--el-disabled-text-color);--el-button-disabled-bg-color:var(--el-fill-color-blank);--el-button-disabled-border-color:var(--el-border-color-light);--el-button-divide-border-color:hsla(0,0%,100%,.5);--el-button-hover-text-color:var(--el-color-primary);--el-button-hover-bg-color:var(--el-color-primary-light-9);--el-button-hover-border-color:var(--el-color-primary-light-7);--el-button-active-text-color:var(--el-button-hover-text-color);--el-button-active-border-color:var(--el-color-primary);--el-button-active-bg-color:var(--el-button-hover-bg-color);--el-button-outline-color:var(--el-color-primary-light-5);--el-button-hover-link-text-color:var(--el-color-info);--el-button-active-color:var(--el-text-color-primary);align-items:center;-webkit-appearance:none;background-color:var(--el-button-bg-color);border:var(--el-border);border-color:var(--el-button-border-color);box-sizing:border-box;color:var(--el-button-text-color);cursor:pointer;display:inline-flex;font-weight:var(--el-button-font-weight);height:32px;justify-content:center;line-height:1;outline:none;text-align:center;transition:.1s;-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-button:hover{background-color:var(--el-button-hover-bg-color);border-color:var(--el-button-hover-border-color);color:var(--el-button-hover-text-color);outline:none}.el-button:active{background-color:var(--el-button-active-bg-color);border-color:var(--el-button-active-border-color);color:var(--el-button-active-text-color);outline:none}.el-button:focus-visible{outline:2px solid var(--el-button-outline-color);outline-offset:1px;transition:outline-offset 0s,outline 0s}.el-button>span{align-items:center;display:inline-flex}.el-button+.el-button{margin-left:12px}.el-button{border-radius:var(--el-border-radius-base);font-size:var(--el-font-size-base)}.el-button,.el-button.is-round{padding:8px 15px}.el-button::-moz-focus-inner{border:0}.el-button [class*=el-icon]+span{margin-left:6px}.el-button [class*=el-icon] 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20px;position:relative}.el-cascader-node.is-selectable.in-active-path{color:var(--el-cascader-menu-text-color)}.el-cascader-node.in-active-path,.el-cascader-node.is-active,.el-cascader-node.is-selectable.in-checked-path{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader-node:not(.is-disabled){cursor:pointer}.el-cascader-node:not(.is-disabled):focus,.el-cascader-node:not(.is-disabled):hover{background:var(--el-cascader-node-background-hover)}.el-cascader-node.is-disabled{color:var(--el-cascader-node-color-disabled);cursor:not-allowed}.el-cascader-node__prefix{left:10px;position:absolute}.el-cascader-node__postfix{position:absolute;right:10px}.el-cascader-node__label{flex:1;overflow:hidden;padding:0 8px;text-align:left;text-overflow:ellipsis;white-space:nowrap}.el-cascader-node>.el-checkbox,.el-cascader-node>.el-radio{margin-right:0}.el-cascader-node>.el-radio .el-radio__label{padding-left:0}.el-cascader{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);display:inline-block;font-size:var(--el-font-size-base);line-height:32px;outline:none;position:relative;vertical-align:middle}.el-cascader:not(.is-disabled):hover .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset;cursor:pointer}.el-cascader .el-input{cursor:pointer;display:flex}.el-cascader .el-input .el-input__inner{cursor:pointer;text-overflow:ellipsis}.el-cascader .el-input .el-input__suffix-inner .el-icon{height:calc(100% - 2px)}.el-cascader .el-input .el-input__suffix-inner .el-icon svg{vertical-align:middle}.el-cascader .el-input .icon-arrow-down{font-size:14px;transition:transform var(--el-transition-duration)}.el-cascader .el-input .icon-arrow-down.is-reverse{transform:rotate(180deg)}.el-cascader .el-input .icon-circle-close:hover{color:var(--el-input-clear-hover-color,var(--el-text-color-secondary))}.el-cascader .el-input.is-focus .el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-focus-border-color,var(--el-color-primary)) inset}.el-cascader--large{font-size:14px;line-height:40px}.el-cascader--small{font-size:12px;line-height:24px}.el-cascader.is-disabled .el-cascader__label{color:var(--el-disabled-text-color);z-index:calc(var(--el-index-normal) + 1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper .el-popper__arrow:before{border:var(--el-cascader-menu-border)}.el-cascader__dropdown.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-cascader__dropdown.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-cascader__dropdown.el-popper{box-shadow:var(--el-cascader-menu-shadow)}.el-cascader__tags{box-sizing:border-box;display:flex;flex-wrap:wrap;left:0;line-height:normal;position:absolute;right:30px;text-align:left;top:50%;transform:translateY(-50%)}.el-cascader__tags .el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags .el-tag{align-items:center;background:var(--el-fill-color);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag.el-tag--dark,.el-cascader__collapse-tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__collapse-tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__collapse-tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__collapse-tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__collapse-tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__collapse-tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__suggestion-panel{border-radius:var(--el-cascader-menu-radius)}.el-cascader__suggestion-list{color:var(--el-cascader-menu-text-color);font-size:var(--el-font-size-base);margin:0;max-height:204px;padding:6px 0;text-align:center}.el-cascader__suggestion-item{align-items:center;cursor:pointer;display:flex;height:34px;justify-content:space-between;outline:none;padding:0 15px;text-align:left}.el-cascader__suggestion-item:focus,.el-cascader__suggestion-item:hover{background:var(--el-cascader-node-background-hover)}.el-cascader__suggestion-item.is-checked{color:var(--el-cascader-menu-selected-text-color);font-weight:700}.el-cascader__suggestion-item>span{margin-right:10px}.el-cascader__empty-text{color:var(--el-cascader-color-empty);margin:10px 0}.el-cascader__search-input{background:transparent;border:none;box-sizing:border-box;color:var(--el-cascader-menu-text-color);flex:1;height:24px;margin:2px 0 2px 11px;min-width:60px;outline:none;padding:0}.el-cascader__search-input::-moz-placeholder{color:transparent}.el-cascader__search-input::placeholder{color:transparent}.el-check-tag{background-color:var(--el-color-info-light-9);border-radius:var(--el-border-radius-base);color:var(--el-color-info);cursor:pointer;display:inline-block;font-size:var(--el-font-size-base);font-weight:700;line-height:var(--el-font-size-base);padding:7px 15px;transition:var(--el-transition-all)}.el-check-tag:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--primary.is-checked{background-color:var(--el-color-primary-light-8);color:var(--el-color-primary)}.el-check-tag.el-check-tag--primary.is-checked:hover{background-color:var(--el-color-primary-light-7)}.el-check-tag.el-check-tag--primary.is-checked.is-disabled{background-color:var(--el-color-primary-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-checked.is-disabled:hover{background-color:var(--el-color-primary-light-8)}.el-check-tag.el-check-tag--primary.is-disabled{background-color:var(--el-color-info-light-9);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--primary.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-check-tag.el-check-tag--success.is-checked{background-color:var(--el-color-success-light-8);color:var(--el-color-success)}.el-check-tag.el-check-tag--success.is-checked:hover{background-color:var(--el-color-success-light-7)}.el-check-tag.el-check-tag--success.is-checked.is-disabled{background-color:var(--el-color-success-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-checked.is-disabled:hover{background-color:var(--el-color-success-light-8)}.el-check-tag.el-check-tag--success.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--success.is-disabled,.el-check-tag.el-check-tag--success.is-disabled:hover{background-color:var(--el-color-success-light-9)}.el-check-tag.el-check-tag--warning.is-checked{background-color:var(--el-color-warning-light-8);color:var(--el-color-warning)}.el-check-tag.el-check-tag--warning.is-checked:hover{background-color:var(--el-color-warning-light-7)}.el-check-tag.el-check-tag--warning.is-checked.is-disabled{background-color:var(--el-color-warning-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-checked.is-disabled:hover{background-color:var(--el-color-warning-light-8)}.el-check-tag.el-check-tag--warning.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--warning.is-disabled,.el-check-tag.el-check-tag--warning.is-disabled:hover{background-color:var(--el-color-warning-light-9)}.el-check-tag.el-check-tag--danger.is-checked{background-color:var(--el-color-danger-light-8);color:var(--el-color-danger)}.el-check-tag.el-check-tag--danger.is-checked:hover{background-color:var(--el-color-danger-light-7)}.el-check-tag.el-check-tag--danger.is-checked.is-disabled{background-color:var(--el-color-danger-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-checked.is-disabled:hover{background-color:var(--el-color-danger-light-8)}.el-check-tag.el-check-tag--danger.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--danger.is-disabled,.el-check-tag.el-check-tag--danger.is-disabled:hover{background-color:var(--el-color-danger-light-9)}.el-check-tag.el-check-tag--error.is-checked{background-color:var(--el-color-error-light-8);color:var(--el-color-error)}.el-check-tag.el-check-tag--error.is-checked:hover{background-color:var(--el-color-error-light-7)}.el-check-tag.el-check-tag--error.is-checked.is-disabled{background-color:var(--el-color-error-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-checked.is-disabled:hover{background-color:var(--el-color-error-light-8)}.el-check-tag.el-check-tag--error.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--error.is-disabled,.el-check-tag.el-check-tag--error.is-disabled:hover{background-color:var(--el-color-error-light-9)}.el-check-tag.el-check-tag--info.is-checked{background-color:var(--el-color-info-light-8);color:var(--el-color-info)}.el-check-tag.el-check-tag--info.is-checked:hover{background-color:var(--el-color-info-light-7)}.el-check-tag.el-check-tag--info.is-checked.is-disabled{background-color:var(--el-color-info-light-8);color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-checked.is-disabled:hover{background-color:var(--el-color-info-light-8)}.el-check-tag.el-check-tag--info.is-disabled{color:var(--el-disabled-text-color);cursor:not-allowed}.el-check-tag.el-check-tag--info.is-disabled,.el-check-tag.el-check-tag--info.is-disabled:hover{background-color:var(--el-color-info-light-9)}.el-checkbox-button{--el-checkbox-button-checked-bg-color:var(--el-color-primary);--el-checkbox-button-checked-text-color:var(--el-color-white);--el-checkbox-button-checked-border-color:var(--el-color-primary);display:inline-block;position:relative}.el-checkbox-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left-color:transparent;bord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15px;position:relative;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-checkbox-button__inner.is-round{padding:8px 15px}.el-checkbox-button__inner:hover{color:var(--el-color-primary)}.el-checkbox-button__inner [class*=el-icon-]{line-height:.9}.el-checkbox-button__inner [class*=el-icon-]+span{margin-left:5px}.el-checkbox-button__original{margin:0;opacity:0;outline:none;position:absolute;z-index:-1}.el-checkbox-button.is-checked .el-checkbox-button__inner{background-color:var(--el-checkbox-button-checked-bg-color);border-color:var(--el-checkbox-button-checked-border-color);box-shadow:-1px 0 0 0 var(--el-color-primary-light-7);color:var(--el-checkbox-button-checked-text-color)}.el-checkbox-button.is-checked:first-child .el-checkbox-button__inner{border-left-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button.is-disabled .el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large .el-checkbox-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-checkbox-button--large .el-checkbox-button__inner.is-round{padding:12px 19px}.el-checkbox-button--small .el-checkbox-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-checkbox-button--small .el-checkbox-button__inner.is-round{padding:5px 11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) .el-checkbox__original:not(:focus-visible){border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__input.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-checked-icon-color);content:"";display:block;height:2px;left:0;position:absolute;right:0;top:5px;transform:scale(.5)}.el-checkbox__input.is-indeterminate .el-checkbox__inner:after{display:none}.el-checkbox__inner{background-color:var(--el-checkbox-bg-color);border:var(--el-checkbox-input-border);border-radius:var(--el-checkbox-border-radius);box-sizing:border-box;display:inline-block;height:var(--el-checkbox-input-height);position:relative;transition:border-color .25s cubic-bezier(.71,-.46,.29,1.46),background-color .25s cubic-bezier(.71,-.46,.29,1.46),outline .25s cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 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29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 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54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 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var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid 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var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker .el-picker-panel__content{margin:0}.el-date-range-picker__header{height:28px;position:relative;text-align:center}.el-date-range-picker__header [class*=arrow-left]{float:left}.el-date-range-picker__header [class*=arrow-right]{float:right}.el-date-range-picker__header div{font-size:16px;font-weight:500;margin-right:50px}.el-date-range-picker__content{box-sizing:border-box;float:left;margin:0;padding:16px;width:50%}.el-date-range-picker__content.is-left{border-right:1px solid var(--el-datepicker-inner-border-color)}.el-date-range-picker__content .el-date-range-picker__header div{margin-left:50px;margin-right:50px}.el-date-range-picker__editors-wrap{box-sizing:border-box;display:table-cell}.el-date-range-picker__editors-wrap.is-right{text-align:right}.el-date-range-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid var(--el-timepicker-inner-border-color,var(--el-border-color-light));box-sizing:border-box;height:36px;line-height:25px;padding:4px;text-align:right}.el-time-panel__btn{background-color:transparent;border:none;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:28px;margin:0 5px;outline:none;padding:0 5px}.el-time-panel__btn.confirm{color:var(--el-timepicker-active-color,var(--el-color-primary));font-weight:800}.el-descriptions{--el-descriptions-table-border:1px solid var(--el-border-color-lighter);--el-descriptions-item-bordered-label-background:var(--el-fill-color-light);box-sizing:border-box;color:var(--el-text-color-primary);font-size:var(--el-font-size-base)}.el-descriptions__header{align-items:center;display:flex;justify-content:space-between;margin-bottom:16px}.el-descriptions__title{color:var(--el-text-color-primary);font-size:16px;font-weight:700}.el-descriptions__body{background-color:var(--el-fill-color-blank)}.el-descriptions__body 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var(--el-menu-base-level-padding)}.horizontal-collapse-transition .el-sub-menu__title 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.el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small .el-radio__inner{height:12px;width:12px}.el-rate{--el-rate-height:20px;--el-rate-font-size:var(--el-font-size-base);--el-rate-icon-size:18px;--el-rate-icon-margin:6px;--el-rate-void-color:var(--el-border-color-darker);--el-rate-fill-color:#f7ba2a;--el-rate-disabled-void-color:var(--el-fill-color);--el-rate-text-color:var(--el-text-color-primary);align-items:center;display:inline-flex;height:32px}.el-rate:active,.el-rate:focus{outline:none}.el-rate__item{color:var(--el-rate-void-color);cursor:pointer;display:inline-block;font-size:0;line-height:normal;position:relative;vertical-align:middle}.el-rate .el-rate__icon{display:inline-block;font-size:var(--el-rate-icon-size);margin-right:var(--el-rate-icon-margin);position:relative;transition:var(--el-transition-duration)}.el-rate .el-rate__icon.hover{transform:scale(1.15)}.el-rate .el-rate__icon .path2{left:0;position:absolute;top:0}.el-rate .el-rate__icon.is-active{color:var(--el-rate-fill-color)}.el-rate__decimal{color:var(--el-rate-fill-color);display:inline-block;overflow:hidden}.el-rate__decimal,.el-rate__decimal--box{left:0;position:absolute;top:0}.el-rate__text{color:var(--el-rate-text-color);font-size:var(--el-rate-font-size);vertical-align:middle}.el-rate--large{height:40px}.el-rate--small{height:24px}.el-rate--small .el-rate__icon{font-size:14px}.el-rate.is-disabled .el-rate__item{color:var(--el-rate-disabled-void-color);cursor:auto}.el-result{--el-result-padding:40px 30px;--el-result-icon-font-size:64px;--el-result-title-font-size:20px;--el-result-title-margin-top:20px;--el-result-subtitle-margin-top:10px;--el-result-extra-margin-top:30px;align-items:center;box-sizing:border-box;display:flex;flex-direction:column;justify-content:center;padding:var(--el-result-padding);text-align:center}.el-result__icon svg{height:var(--el-result-icon-font-size);width:var(--el-result-icon-font-size)}.el-result__title{margin-top:var(--el-result-title-margin-top)}.el-result__title p{color:var(--el-text-color-primary);font-size:var(--el-result-title-font-size);line-height:1.3;margin:0}.el-result__subtitle{margin-top:var(--el-result-subtitle-margin-top)}.el-result__subtitle p{color:var(--el-text-color-regular);font-size:var(--el-font-size-base);line-height:1.3;margin:0}.el-result__extra{margin-top:var(--el-result-extra-margin-top)}.el-result .icon-primary{--el-result-color:var(--el-color-primary);color:var(--el-result-color)}.el-result .icon-success{--el-result-color:var(--el-color-success);color:var(--el-result-color)}.el-result .icon-warning{--el-result-color:var(--el-color-warning);color:var(--el-result-color)}.el-result .icon-danger{--el-result-color:var(--el-color-danger);color:var(--el-result-color)}.el-result .icon-error{--el-result-color:var(--el-color-error);color:var(--el-result-color)}.el-result 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.el-tag__close{color:var(--el-tag-text-color);flex-shrink:0}.el-tag .el-tag__close:hover{background-color:var(--el-tag-hover-color);color:var(--el-color-white)}.el-tag.el-tag--primary{--el-tag-text-color:var(--el-color-primary)}.el-tag.el-tag--success{--el-tag-text-color:var(--el-color-success)}.el-tag.el-tag--warning{--el-tag-text-color:var(--el-color-warning)}.el-tag.el-tag--danger{--el-tag-text-color:var(--el-color-danger)}.el-tag.el-tag--error{--el-tag-text-color:var(--el-color-error)}.el-tag.el-tag--info{--el-tag-text-color:var(--el-color-info)}.el-tag .el-icon{border-radius:50%;cursor:pointer;font-size:calc(var(--el-icon-size) - 2px);height:var(--el-icon-size);width:var(--el-icon-size)}.el-tag 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0;min-width:0}.time-select .el-picker-panel__content{margin:0;max-height:200px}.time-select-item{font-size:14px;line-height:20px;padding:8px 10px}.time-select-item.disabled{color:var(--el-datepicker-border-color);cursor:not-allowed}.time-select-item:hover{background-color:var(--el-fill-color-light);cursor:pointer;font-weight:700}.time-select .time-select-item.selected:not(.disabled){color:var(--el-color-primary);font-weight:700}.el-timeline-item{padding-bottom:20px;position:relative}.el-timeline-item__wrapper{padding-left:28px;position:relative;top:-3px}.el-timeline-item__tail{border-left:2px solid var(--el-timeline-node-color);height:100%;left:4px;position:absolute}.el-timeline-item .el-timeline-item__icon{color:var(--el-color-white);font-size:var(--el-font-size-small)}.el-timeline-item__node{align-items:center;background-color:var(--el-timeline-node-color);border-color:var(--el-timeline-node-color);border-radius:50%;box-sizing:border-box;display:flex;justify-content:center;position:absolute}.el-timeline-item__node--normal{height:var(--el-timeline-node-size-normal);left:-1px;width:var(--el-timeline-node-size-normal)}.el-timeline-item__node--large{height:var(--el-timeline-node-size-large);left:-2px;width:var(--el-timeline-node-size-large)}.el-timeline-item__node.is-hollow{background:var(--el-color-white);border-style:solid;border-width:2px}.el-timeline-item__node--primary{background-color:var(--el-color-primary);border-color:var(--el-color-primary)}.el-timeline-item__node--success{background-color:var(--el-color-success);border-color:var(--el-color-success)}.el-timeline-item__node--warning{background-color:var(--el-color-warning);border-color:var(--el-color-warning)}.el-timeline-item__node--danger{background-color:var(--el-color-danger);border-color:var(--el-color-danger)}.el-timeline-item__node--info{background-color:var(--el-color-info);border-color:var(--el-color-info)}.el-timeline-item__dot{align-items:center;display:flex;justify-content:center;position:absolute}.el-timeline-item__content{color:var(--el-text-color-primary)}.el-timeline-item__timestamp{color:var(--el-text-color-secondary);font-size:var(--el-font-size-small);line-height:1}.el-timeline-item__timestamp.is-top{margin-bottom:8px;padding-top:4px}.el-timeline-item__timestamp.is-bottom{margin-top:8px}.el-timeline{--el-timeline-node-size-normal:12px;--el-timeline-node-size-large:14px;--el-timeline-node-color:var(--el-border-color-light);font-size:var(--el-font-size-base);list-style:none;margin:0}.el-timeline 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srcset="https://static.igem.wiki/teams/5358/banner/wetlab.webp 1x, https://static.igem.wiki/teams/5358/banner/wetlab.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/wetlab.webp" data-v-0a95154d><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg" data-v-0a95154d>Results</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10" data-v-0a95154d><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[400px] md:mr-10" data-v-0a95154d><h1 class="mt-0" data-v-0a95154d>TOC</h1><ul data-v-0a95154d><!--[--><li class="my-5" data-v-0a95154d><a class="cursor-pointer" data-v-0a95154d>1. pUC19-PrecA-BsFbFp-BL21</a></li><li class="my-5" data-v-0a95154d><a class="cursor-pointer" data-v-0a95154d>2. pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21</a></li><li class="my-5" data-v-0a95154d><a class="cursor-pointer" data-v-0a95154d>3. pUC19-PBAD-N-T7RNAP-ER-LBD intein-C-T7RNAP-BsFbFp</a></li><li class="my-5" data-v-0a95154d><a class="cursor-pointer" data-v-0a95154d>4. psB1C3-Pj23100-LuxI-BL21 and psB1C3-Plux-LuxR-EGFP-BL21</a></li><!--]--></ul></aside><div class="content" data-v-0a95154d><p><!--[-->Endocrine disruptors are a class of chemicals that can interfere with the normal function of the endocrine system, and they may mimic or inhibit the effects of natural hormones, thereby affecting the physiological processes of the body. These substances are widely found in plastics, pesticides, industrial chemicals, and daily consumer goods. They enter the human body through the food chain or through direct contact, interfering with hormonal balance, and may cause reproductive health problems, developmental disorders, metabolic disorders and even some types of cancer.<!--]--></p><p><!--[-->When endocrine disruptors affect hormone activity, they may also induce cellular oxidative stress because they increase the generation of reactive oxygen species (ROS), highly reactive chemicals that damage cellular structures, including proteins, lipids, and DNA. Long-term oxidative stress accelerates cellular aging and may also lead to male infertility and the development of multiple chronic diseases. Thus, there is a close link between exposure to endocrine disruptors and cellular oxidative damage, a potential promoter to multiple health problems. Therefore, it is necessary to detect the endocrine interference effects of endocrine disruptors and oxidative damage effects in the relevant location samples.<!--]--></p><h2 id="_1-puc19-preca-bsfbfp-bl21"><!--[-->1. pUC19-PrecA-BsFbFp-BL21<!--]--></h2><h3 id="strain-construction"><!--[-->Strain construction<!--]--></h3><p><!--[-->We plan to design a portable microbial sensor, in which the sensor chamber will be maintained in a nearly anoxic state. Under anoxic conditions, the fluorescence intensity of EGFP diminishes, and its detection limit decreases; therefore, traditional EGFP is not the optimal choice for our application. We have constructed the PrecA-BsFbFP circuit, where BsFbFP is an anoxic fluorescent protein that exhibits fluorescence intensity nearly equivalent to that of EGFP under anoxic conditions, while significantly improving the detection limit. Consequently, we will utilize BsFbFP as the expression product of our engineered strain for detection characterization.<!--]--></p><p><!--[-->We obtained the PrecA-BsFbFp circuit and the recA-eGFP (hereafter referred to as wild) circuit through PCR.The gene circuits were ligated to the pUC19 vector using enzyme ligation and transformed into BL21 competent cells . The transformed cells were spread onto LB solid medium supplemented with ampicillin. Single colonies that grew were selected for amplification and sent to Jinweizhi (Suzhou) Biochemistry Co. for sequencing, and the remaining bacteria were preserved in glycerol.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/fig1.png 1x, https://static.igem.wiki/teams/5358/experiment/fig1.png 2x" src="https://static.igem.wiki/teams/5358/experiment/fig1.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/bsfbfp.png 1x, https://static.igem.wiki/teams/5358/experiment/bsfbfp.png 2x" src="https://static.igem.wiki/teams/5358/experiment/bsfbfp.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 1. Results of the enzyme digestion verification experiment for pUC19-PrecA-BsFbFP-BL21.</span><!--]--></p><h3 id="characterization-of-experiments"><!--[-->Characterization of experiments<!--]--></h3><p><!--[-->Firstly, we performed screening of the pUC19-PrecA-BsFbFp-BL21. The transformed wild-type bacteria were cultured on a shaker until the OD value reached 0.2, followed by the addition of NA for induction for 2 h. In aerobic conditions, BsFbFp is significantly less characterized than EGFP, while the RFU is close, and BsFbFp has a high detection limit, indicating that BsFbFp is an excellent choice for characterization in anaerobic conditions.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/o2-fluo.png 1x, https://static.igem.wiki/teams/5358/experiment/o2-fluo.png 2x" src="https://static.igem.wiki/teams/5358/experiment/o2-fluo.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/o2-rfu.png 1x, https://static.igem.wiki/teams/5358/experiment/o2-rfu.png 2x" src="https://static.igem.wiki/teams/5358/experiment/o2-rfu.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 2. Characterization results of BsFbFP and EGFP under anoxic and NA gradient injury conditions.</span><!--]--></p><p><!--[-->We have characterized the pUC19-PrecA-BsFbFp-BL21 under anaerobic conditions.The transformed wild-type bacteria were cultured on a shaker until the OD value reached 0.2, followed by the addition of NA for induction for 2 h.As the concentration of NA increased, the fluorescence intensity of engineering strains increased accordingly. Several experiments proved that the RFU value increased with the concentration of NA in the anaerobic environment.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/10-rfu.png 1x, https://static.igem.wiki/teams/5358/experiment/10-rfu.png 2x" src="https://static.igem.wiki/teams/5358/experiment/10-rfu.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/10-rfu-more.png 1x, https://static.igem.wiki/teams/5358/experiment/10-rfu-more.png 2x" src="https://static.igem.wiki/teams/5358/experiment/10-rfu-more.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 3. Fluorescence intensity characterization of BsFbFP under NA gradient injury conditions in 10-lianpao chip cultures, expressed as RFU.</span><!--]--></p><p><!--[-->Later, we compared the characterization difference between DH5α strain and BL21 strain, and showed that the BL21 strain has higher sensitivity to injury, reflecting its advantage as a representative strain.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/o2-fluo-more.png 1x, https://static.igem.wiki/teams/5358/experiment/o2-fluo-more.png 2x" src="https://static.igem.wiki/teams/5358/experiment/o2-fluo-more.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 4. Fluorescence characterization gradients of DH5α and BL21 under aerobic and NA gradient injury conditions.</span><!--]--></p><h2 id="_2-pet28-preca-hrpr-hrps-phrpl-bsfbfp-bl21"><!--[-->2. pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21<!--]--></h2><p><!--[-->The gene circuits were ligated to the pET28 vector using enzyme ligation and transformed into BL21 competent cells . The transformed cells were spread onto LB solid medium supplemented with ampicillin.Preserved strains were stored in glycerol.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/hrp.png 1x, https://static.igem.wiki/teams/5358/experiment/hrp.png 2x" src="https://static.igem.wiki/teams/5358/experiment/hrp.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/2-enzyme-digestion.png 1x, https://static.igem.wiki/teams/5358/experiment/2-enzyme-digestion.png 2x" src="https://static.igem.wiki/teams/5358/experiment/2-enzyme-digestion.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 5. Results of the enzyme digestion verification experiment for pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21.</span><!--]--></p><h2 id="_3-puc19-pbad-n-t7rnap-er-lbd-intein-c-t7rnap-bsfbfp"><!--[-->3. pUC19-PBAD-N-T7RNAP-ER-LBD intein-C-T7RNAP-BsFbFp<!--]--></h2><p><!--[-->The gene sequencing conducted by our company has successfully validated the synthesis of the intein of the biological circuit.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/fig3.png 1x, https://static.igem.wiki/teams/5358/experiment/fig3.png 2x" src="https://static.igem.wiki/teams/5358/experiment/fig3.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/gene-squencing.png 1x, https://static.igem.wiki/teams/5358/experiment/gene-squencing.png 2x" src="https://static.igem.wiki/teams/5358/experiment/gene-squencing.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 6. Gene sequencing results of the ER-LBD intein.</span><!--]--></p><h2 id="_4-psb1c3-pj23100-luxi-bl21-and-psb1c3-plux-luxr-egfp-bl21"><!--[-->4. psB1C3-Pj23100-LuxI-BL21 and psB1C3-Plux-LuxR-EGFP-BL21<!--]--></h2><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/fig4.png 1x, https://static.igem.wiki/teams/5358/experiment/fig4.png 2x" src="https://static.igem.wiki/teams/5358/experiment/fig4.png"><!--]--></p><p><!--[-->We obtained by PCR the Pj233100, Plux-LuxR, LuxI, and EGFP and ligated it to the psB1C3 plasmid into E. coli BL21. The transformed cells were spread onto LB solid medium supplemented with chloramphenicol. Recombinants were used for characterization experiments and stored in glycerol.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/pcr.png 1x, https://static.igem.wiki/teams/5358/experiment/pcr.png 2x" src="https://static.igem.wiki/teams/5358/experiment/pcr.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 7. PCR products of the Pj23100-LuxI fragment and the Plux-LuxR-EGFP fragment.</span><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/lux-digestion.png 1x, https://static.igem.wiki/teams/5358/experiment/lux-digestion.png 2x" src="https://static.igem.wiki/teams/5358/experiment/lux-digestion.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 8. Enzyme digestion verification of psB1C3-Pj23100-LuxI-BL21 and Plux-LuxR-EGFP-BL21.</span><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/ligation.png 1x, https://static.igem.wiki/teams/5358/experiment/ligation.png 2x" src="https://static.igem.wiki/teams/5358/experiment/ligation.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 9. Enzyme Ligation Product of psB1C3-Pj23100-LuxI-BL21 and Plux-LuxR-EGFP-BL21.</span><!--]--></p></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a aria-current="page" href="/bit/result" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/result/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-WfQ6VD3q4y":11},null,"/result",1727868995288]</script>
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src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div class="grow"></div><!--[--><span class="el-avatar el-avatar--icon el-avatar--circle self-center cursor-pointer el-tooltip__trigger el-tooltip__trigger" style=""><i class="el-icon" style=""><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M160 448a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zM160 896a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32z"></path></svg><!--]--></i></span><!--teleport start--><!--teleport end--><!--]--></ul></header><div class="pt-[8rem]"><!--[--><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="cover" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/wetlab.webp 1x, https://static.igem.wiki/teams/5358/banner/wetlab.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/wetlab.webp" data-v-0a95154d><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg" data-v-0a95154d>Results</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10" data-v-0a95154d><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[400px] md:mr-10" data-v-0a95154d><h1 class="mt-0" data-v-0a95154d>TOC</h1><ul data-v-0a95154d><!--[--><li class="my-5" data-v-0a95154d><a class="cursor-pointer" data-v-0a95154d>1. pUC19-PrecA-BsFbFp-BL21</a></li><li class="my-5" data-v-0a95154d><a class="cursor-pointer" data-v-0a95154d>2. pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21</a></li><li class="my-5" data-v-0a95154d><a class="cursor-pointer" data-v-0a95154d>3. pUC19-PBAD-N-T7RNAP-ER-LBD intein-C-T7RNAP-BsFbFp</a></li><li class="my-5" data-v-0a95154d><a class="cursor-pointer" data-v-0a95154d>4. psB1C3-Pj23100-LuxI-BL21 and psB1C3-Plux-LuxR-EGFP-BL21</a></li><!--]--></ul></aside><div class="content" data-v-0a95154d><p><!--[-->Endocrine disruptors are a class of chemicals that can interfere with the normal function of the endocrine system, and they may mimic or inhibit the effects of natural hormones, thereby affecting the physiological processes of the body. These substances are widely found in plastics, pesticides, industrial chemicals, and daily consumer goods. They enter the human body through the food chain or through direct contact, interfering with hormonal balance, and may cause reproductive health problems, developmental disorders, metabolic disorders and even some types of cancer.<!--]--></p><p><!--[-->When endocrine disruptors affect hormone activity, they may also induce cellular oxidative stress because they increase the generation of reactive oxygen species (ROS), highly reactive chemicals that damage cellular structures, including proteins, lipids, and DNA. Long-term oxidative stress accelerates cellular aging and may also lead to male infertility and the development of multiple chronic diseases. Thus, there is a close link between exposure to endocrine disruptors and cellular oxidative damage, a potential promoter to multiple health problems. Therefore, it is necessary to detect the endocrine interference effects of endocrine disruptors and oxidative damage effects in the relevant location samples.<!--]--></p><h2 id="_1-puc19-preca-bsfbfp-bl21"><!--[-->1. pUC19-PrecA-BsFbFp-BL21<!--]--></h2><h3 id="strain-construction"><!--[-->Strain construction<!--]--></h3><p><!--[-->We plan to design a portable microbial sensor, in which the sensor chamber will be maintained in a nearly anoxic state. Under anoxic conditions, the fluorescence intensity of EGFP diminishes, and its detection limit decreases; therefore, traditional EGFP is not the optimal choice for our application. We have constructed the PrecA-BsFbFP circuit, where BsFbFP is an anoxic fluorescent protein that exhibits fluorescence intensity nearly equivalent to that of EGFP under anoxic conditions, while significantly improving the detection limit. Consequently, we will utilize BsFbFP as the expression product of our engineered strain for detection characterization.<!--]--></p><p><!--[-->We obtained the PrecA-BsFbFp circuit and the recA-eGFP (hereafter referred to as wild) circuit through PCR.The gene circuits were ligated to the pUC19 vector using enzyme ligation and transformed into BL21 competent cells . The transformed cells were spread onto LB solid medium supplemented with ampicillin. Single colonies that grew were selected for amplification and sent to Jinweizhi (Suzhou) Biochemistry Co. for sequencing, and the remaining bacteria were preserved in glycerol.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/fig1.png 1x, https://static.igem.wiki/teams/5358/experiment/fig1.png 2x" src="https://static.igem.wiki/teams/5358/experiment/fig1.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/bsfbfp.png 1x, https://static.igem.wiki/teams/5358/experiment/bsfbfp.png 2x" src="https://static.igem.wiki/teams/5358/experiment/bsfbfp.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 1. Results of the enzyme digestion verification experiment for pUC19-PrecA-BsFbFP-BL21.</span><!--]--></p><h3 id="characterization-of-experiments"><!--[-->Characterization of experiments<!--]--></h3><p><!--[-->Firstly, we performed screening of the pUC19-PrecA-BsFbFp-BL21. The transformed wild-type bacteria were cultured on a shaker until the OD value reached 0.2, followed by the addition of NA for induction for 2 h. In aerobic conditions, BsFbFp is significantly less characterized than EGFP, while the RFU is close, and BsFbFp has a high detection limit, indicating that BsFbFp is an excellent choice for characterization in anaerobic conditions.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/o2-fluo.png 1x, https://static.igem.wiki/teams/5358/experiment/o2-fluo.png 2x" src="https://static.igem.wiki/teams/5358/experiment/o2-fluo.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/o2-rfu.png 1x, https://static.igem.wiki/teams/5358/experiment/o2-rfu.png 2x" src="https://static.igem.wiki/teams/5358/experiment/o2-rfu.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 2. Characterization results of BsFbFP and EGFP under anoxic and NA gradient injury conditions.</span><!--]--></p><p><!--[-->We have characterized the pUC19-PrecA-BsFbFp-BL21 under anaerobic conditions.The transformed wild-type bacteria were cultured on a shaker until the OD value reached 0.2, followed by the addition of NA for induction for 2 h.As the concentration of NA increased, the fluorescence intensity of engineering strains increased accordingly. Several experiments proved that the RFU value increased with the concentration of NA in the anaerobic environment.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/10-rfu.png 1x, https://static.igem.wiki/teams/5358/experiment/10-rfu.png 2x" src="https://static.igem.wiki/teams/5358/experiment/10-rfu.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/10-rfu-more.png 1x, https://static.igem.wiki/teams/5358/experiment/10-rfu-more.png 2x" src="https://static.igem.wiki/teams/5358/experiment/10-rfu-more.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 3. Fluorescence intensity characterization of BsFbFP under NA gradient injury conditions in 10-lianpao chip cultures, expressed as RFU.</span><!--]--></p><p><!--[-->Later, we compared the characterization difference between DH5α strain and BL21 strain, and showed that the BL21 strain has higher sensitivity to injury, reflecting its advantage as a representative strain.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/o2-fluo-more.png 1x, https://static.igem.wiki/teams/5358/experiment/o2-fluo-more.png 2x" src="https://static.igem.wiki/teams/5358/experiment/o2-fluo-more.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 4. Fluorescence characterization gradients of DH5α and BL21 under aerobic and NA gradient injury conditions.</span><!--]--></p><h2 id="_2-pet28-preca-hrpr-hrps-phrpl-bsfbfp-bl21"><!--[-->2. pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21<!--]--></h2><p><!--[-->The gene circuits were ligated to the pET28 vector using enzyme ligation and transformed into BL21 competent cells . The transformed cells were spread onto LB solid medium supplemented with ampicillin.Preserved strains were stored in glycerol.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/hrp.png 1x, https://static.igem.wiki/teams/5358/experiment/hrp.png 2x" src="https://static.igem.wiki/teams/5358/experiment/hrp.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/2-enzyme-digestion.png 1x, https://static.igem.wiki/teams/5358/experiment/2-enzyme-digestion.png 2x" src="https://static.igem.wiki/teams/5358/experiment/2-enzyme-digestion.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 5. Results of the enzyme digestion verification experiment for pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21.</span><!--]--></p><h2 id="_3-puc19-pbad-n-t7rnap-er-lbd-intein-c-t7rnap-bsfbfp"><!--[-->3. pUC19-PBAD-N-T7RNAP-ER-LBD intein-C-T7RNAP-BsFbFp<!--]--></h2><p><!--[-->The gene sequencing conducted by our company has successfully validated the synthesis of the intein of the biological circuit.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/fig3.png 1x, https://static.igem.wiki/teams/5358/experiment/fig3.png 2x" src="https://static.igem.wiki/teams/5358/experiment/fig3.png"><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/gene-squencing.png 1x, https://static.igem.wiki/teams/5358/experiment/gene-squencing.png 2x" src="https://static.igem.wiki/teams/5358/experiment/gene-squencing.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 6. Gene sequencing results of the ER-LBD intein.</span><!--]--></p><h2 id="_4-psb1c3-pj23100-luxi-bl21-and-psb1c3-plux-luxr-egfp-bl21"><!--[-->4. psB1C3-Pj23100-LuxI-BL21 and psB1C3-Plux-LuxR-EGFP-BL21<!--]--></h2><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/fig4.png 1x, https://static.igem.wiki/teams/5358/experiment/fig4.png 2x" src="https://static.igem.wiki/teams/5358/experiment/fig4.png"><!--]--></p><p><!--[-->We obtained by PCR the Pj233100, Plux-LuxR, LuxI, and EGFP and ligated it to the psB1C3 plasmid into E. coli BL21. The transformed cells were spread onto LB solid medium supplemented with chloramphenicol. Recombinants were used for characterization experiments and stored in glycerol.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/pcr.png 1x, https://static.igem.wiki/teams/5358/experiment/pcr.png 2x" src="https://static.igem.wiki/teams/5358/experiment/pcr.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 7. PCR products of the Pj23100-LuxI fragment and the Plux-LuxR-EGFP fragment.</span><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/lux-digestion.png 1x, https://static.igem.wiki/teams/5358/experiment/lux-digestion.png 2x" src="https://static.igem.wiki/teams/5358/experiment/lux-digestion.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 8. Enzyme digestion verification of psB1C3-Pj23100-LuxI-BL21 and Plux-LuxR-EGFP-BL21.</span><!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="img" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/experiment/ligation.png 1x, https://static.igem.wiki/teams/5358/experiment/ligation.png 2x" src="https://static.igem.wiki/teams/5358/experiment/ligation.png"><!--]--></p><p><!--[--><span class="fig-title">Figure 9. Enzyme Ligation Product of psB1C3-Pj23100-LuxI-BL21 and Plux-LuxR-EGFP-BL21.</span><!--]--></p></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a href="/bit/safety" class="mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a aria-current="page" href="/bit/result" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" data-src="/bit/result/_payload.json">[{"state":1,"once":7,"_errors":9,"serverRendered":5,"path":12,"prerenderedAt":13},["Reactive",2],{"$scolor-mode":3},{"preference":4,"value":4,"unknown":5,"forced":6},"system",true,false,["Reactive",8],["Set"],["Reactive",10],{"content-query-WfQ6VD3q4y":11},null,"/result",1727871092328]</script>
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diff --git a/.output/public/safety/_payload.json b/.output/public/safety/_payload.json
index 9f7a2c0f7e471b3447eee5e5014c70892b18fd1f..2fcd2d849aadf1e7c68429008b83e16c28bd199c 100644
--- a/.output/public/safety/_payload.json
+++ b/.output/public/safety/_payload.json
@@ -1 +1 @@
-[{"data":1,"prerenderedAt":357},["Reactive",2],{"content-query-zna4rCgBuI":3},{"_path":4,"_dir":5,"_draft":6,"_partial":6,"_locale":5,"title":7,"description":8,"body":9,"_type":352,"_id":353,"_source":354,"_file":355,"_stem":78,"_extension":356},"/project-safety","",false,"Safety","Synthetic biology has the ability to change the world in amazing ways. From treating cancer to alleviating the climate crisis, we do have the ability to build a better world at iGEM. However, by working with some unpredictable organisms, we must also understand that the good works we create can actually cause harm. When looking at the big picture, we must also understand that we must be carefully every day. As researchers, we need to manage the community and environmental risks.",{"type":10,"children":11,"toc":340},"root",[12,19,26,76,82,89,94,99,105,134,140,145,154,159,164,182,188,202,207,235,240,258,263,291,296,301,312,317,326,331],{"type":13,"tag":14,"props":15,"children":16},"element","p",{},[17],{"type":18,"value":8},"text",{"type":13,"tag":20,"props":21,"children":23},"h2",{"id":22},"the-rules-and-policies-of-the-igem",[24],{"type":18,"value":25},"The rules and policies of the iGEM",{"type":13,"tag":27,"props":28,"children":29},"ol",{},[30,36,41,46,51,56,61,66,71],{"type":13,"tag":31,"props":32,"children":33},"li",{},[34],{"type":18,"value":35},"Release Beyond Containment: You cannot release or deploy any genetically modified organisms outside the lab. You must Check In before bringing any product of synthetic biology outside of the lab.",{"type":13,"tag":31,"props":37,"children":38},{},[39],{"type":18,"value":40},"White List: You must Check In before beginning experiments with any organism, part, or activity that is not on the White List.",{"type":13,"tag":31,"props":42,"children":43},{},[44],{"type":18,"value":45},"No Human Experimentation: Testing your project on humans (including yourselves) is strictly prohibited. You must check in before beginning any laboratory experiments involving humans or human samples (including but not limited to blood, DNA, other bodily specimens, and health or psychological outcomes).",{"type":13,"tag":31,"props":47,"children":48},{},[49],{"type":18,"value":50},"Animal Use: You must Check In and submit an Animal Use Form before beginning any experiments involving animals or animal samples. This includes vertebrates (e.g. rats, mice, guinea pigs, hamsters, amphibians, and fishes) and higher order invertebrates(e.g. cuttlefish, octopus, squid, lobster, bees, or American or German cockroaches).",{"type":13,"tag":31,"props":52,"children":53},{},[54],{"type":18,"value":55},"Human Subjects Research: All human subjects research (e.g.surveys, interviews, public engagement) you carry out must be done in accordance with relevant laws and regulations, as well as any institutional rules or guidance.",{"type":13,"tag":31,"props":57,"children":58},{},[59],{"type":18,"value":60},"Antimicrobial Resistance: You must Check In before beginning experiments likely to increase the risk posed by antimicrobial resistance (e.g. by using novel resistance factors, adding known factors into new organisms, or resistance factors not previously used in your facility).",{"type":13,"tag":31,"props":62,"children":63},{},[64],{"type":18,"value":65},"Gene Drive: Gene Drive is not allowed in iGEM projects without a special exception from the Safety & Security Committee.",{"type":13,"tag":31,"props":67,"children":68},{},[69],{"type":18,"value":70},"Coronavirus: Any work using live SARS-CoV-2 virus(the causative agent of COVID-19) must follow World Health Organization (WHO) guidance, in addition to all local and institutional rules. WHO guidance may be stricter than local safety rules. You must Check In before beginning experiments with live SARS-CoV-2 or parts from SARS-CoV-2.",{"type":13,"tag":31,"props":72,"children":73},{},[74],{"type":18,"value":75},"Environmental Samples: As long as parts or organisms are not isolated from them, samples from the environment, the food industry, or other non- traditional suppliers may be used in your experiments You must Check In before using any parts organisms obtained from outside the lab or from non- traditional suppliers.",{"type":13,"tag":20,"props":77,"children":79},{"id":78},"project-safety",[80],{"type":18,"value":81},"Project safety",{"type":13,"tag":83,"props":84,"children":86},"h3",{"id":85},"safety-of-project-design",[87],{"type":18,"value":88},"Safety of project design",{"type":13,"tag":14,"props":90,"children":91},{},[92],{"type":18,"value":93},"Our project employs the insertion of RecA intein into T7 RNAP to detect endocrine disruption effects and utilizes the SOS response mechanism of Escherichia coli to detect DNA damage. Additionally, the LuxI/R system is introduced to achieve an AND gate detection of both effects. Furthermore, the Hrp gene regulation system and BsFbFp fluorescent gene are combined to amplify the transcriptional signal and ensure effective signal expression under anaerobic conditions; in the hardware section, we have designed a high-throughput platform for automated sample detection. Throughout our entire experimental process, our team has conducted experiments using qualified E. coli strains such as BL21, DH5a, and MG1655, as well as plasmid vectors pUC19 and pET28. These E. coli strains are classified as Biosafety Level I and have been handled in a sterile environment.",{"type":13,"tag":14,"props":95,"children":96},{},[97],{"type":18,"value":98},"According to the WHO Laboratory Biosafety Manual, the risks involved in our experiments are Level I (no risk or very low risk).",{"type":13,"tag":83,"props":100,"children":102},{"id":101},"project-hardware-security",[103],{"type":18,"value":104},"Project Hardware Security",{"type":13,"tag":106,"props":107,"children":108},"ul",{},[109,114,119,124,129],{"type":13,"tag":31,"props":110,"children":111},{},[112],{"type":18,"value":113},"Disposable Reaction Vessels: We utilize disposable PCR tubes as reaction vessels, which means that there is no need for contact with the biological system at the start of the reaction, thus eliminating concerns about genetic modification organism contamination.",{"type":13,"tag":31,"props":115,"children":116},{},[117],{"type":18,"value":118},"No Direct Contact: Users only need to add the sample liquid to the reaction chamber; unless necessary, it is not recommended to open our device.",{"type":13,"tag":31,"props":120,"children":121},{},[122],{"type":18,"value":123},"Sealed Containers: The reaction chamber is completely sealed, so unless the user intentionally breaks it, there will be no opportunity for the user to come into contact with the reagents inside.",{"type":13,"tag":31,"props":125,"children":126},{},[127],{"type":18,"value":128},"Automated Control: All operations after adding the sample can be controlled automatically through a mobile app, without the need for manual operation of the device.",{"type":13,"tag":31,"props":130,"children":131},{},[132],{"type":18,"value":133},"Modular Design: If the device is damaged, only the specific part needs to be replaced, without the need for disassembly.",{"type":13,"tag":83,"props":135,"children":137},{"id":136},"engineering-bacterial-system",[138],{"type":18,"value":139},"Engineering Bacterial System",{"type":13,"tag":14,"props":141,"children":142},{},[143],{"type":18,"value":144},"Our project utilizes Escherichia coli as an engineered strain, relying on genetically designed circuits to specifically identify trace amounts of endocrine disruptors and elicit a response. 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As researchers, we need to manage the community and environmental risks.",{"type":10,"children":11,"toc":340},"root",[12,19,26,76,82,89,94,99,105,134,140,145,154,159,164,182,188,202,207,235,240,258,263,291,296,301,312,317,326,331],{"type":13,"tag":14,"props":15,"children":16},"element","p",{},[17],{"type":18,"value":8},"text",{"type":13,"tag":20,"props":21,"children":23},"h2",{"id":22},"the-rules-and-policies-of-the-igem",[24],{"type":18,"value":25},"The rules and policies of the iGEM",{"type":13,"tag":27,"props":28,"children":29},"ol",{},[30,36,41,46,51,56,61,66,71],{"type":13,"tag":31,"props":32,"children":33},"li",{},[34],{"type":18,"value":35},"Release Beyond Containment: You cannot release or deploy any genetically modified organisms outside the lab. 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.el-button--info:not(:first-child):not(:last-child){border-left-color:var(--el-button-divide-border-color);border-right-color:var(--el-button-divide-border-color)}.el-button{--el-button-font-weight:var(--el-font-weight-primary);--el-button-border-color:var(--el-border-color);--el-button-bg-color:var(--el-fill-color-blank);--el-button-text-color:var(--el-text-color-regular);--el-button-disabled-text-color:var(--el-disabled-text-color);--el-button-disabled-bg-color:var(--el-fill-color-blank);--el-button-disabled-border-color:var(--el-border-color-light);--el-button-divide-border-color:hsla(0,0%,100%,.5);--el-button-hover-text-color:var(--el-color-primary);--el-button-hover-bg-color:var(--el-color-primary-light-9);--el-button-hover-border-color:var(--el-color-primary-light-7);--el-button-active-text-color:var(--el-button-hover-text-color);--el-button-active-border-color:var(--el-color-primary);--el-button-active-bg-color:var(--el-button-hover-bg-color);--el-button-outline-color:var(--el-color-primary-light-5);--el-button-hover-link-text-color:var(--el-color-info);--el-button-active-color:var(--el-text-color-primary);align-items:center;-webkit-appearance:none;background-color:var(--el-button-bg-color);border:var(--el-border);border-color:var(--el-button-border-color);box-sizing:border-box;color:var(--el-button-text-color);cursor:pointer;display:inline-flex;font-weight:var(--el-button-font-weight);height:32px;justify-content:center;line-height:1;outline:none;text-align:center;transition:.1s;-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-button:hover{background-color:var(--el-button-hover-bg-color);border-color:var(--el-button-hover-border-color);color:var(--el-button-hover-text-color);outline:none}.el-button:active{background-color:var(--el-button-active-bg-color);border-color:var(--el-button-active-border-color);color:var(--el-button-active-text-color);outline:none}.el-button:focus-visible{outline:2px 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1)}.el-cascader__dropdown{--el-cascader-menu-text-color:var(--el-text-color-regular);--el-cascader-menu-selected-text-color:var(--el-color-primary);--el-cascader-menu-fill:var(--el-bg-color-overlay);--el-cascader-menu-font-size:var(--el-font-size-base);--el-cascader-menu-radius:var(--el-border-radius-base);--el-cascader-menu-border:solid 1px var(--el-border-color-light);--el-cascader-menu-shadow:var(--el-box-shadow-light);--el-cascader-node-background-hover:var(--el-fill-color-light);--el-cascader-node-color-disabled:var(--el-text-color-placeholder);--el-cascader-color-empty:var(--el-text-color-placeholder);--el-cascader-tag-background:var(--el-fill-color);border-radius:var(--el-cascader-menu-radius);font-size:var(--el-cascader-menu-font-size)}.el-cascader__dropdown.el-popper{background:var(--el-cascader-menu-fill)}.el-cascader__dropdown.el-popper,.el-cascader__dropdown.el-popper 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.el-tag{align-items:center;background:var(--el-cascader-tag-background);display:inline-flex;margin:2px 0 2px 6px;max-width:100%;text-overflow:ellipsis}.el-cascader__tags .el-tag.el-tag--dark,.el-cascader__tags .el-tag.el-tag--plain{background-color:var(--el-tag-bg-color)}.el-cascader__tags .el-tag:not(.is-hit){border-color:transparent}.el-cascader__tags .el-tag:not(.is-hit).el-tag--dark,.el-cascader__tags .el-tag:not(.is-hit).el-tag--plain{border-color:var(--el-tag-border-color)}.el-cascader__tags .el-tag>span{flex:1;overflow:hidden;text-overflow:ellipsis}.el-cascader__tags .el-tag .el-icon-close{background-color:var(--el-text-color-placeholder);color:var(--el-color-white);flex:none}.el-cascader__tags .el-tag .el-icon-close:hover{background-color:var(--el-text-color-secondary)}.el-cascader__tags.is-validate{right:55px}.el-cascader__collapse-tags{white-space:normal;z-index:var(--el-index-normal)}.el-cascader__collapse-tags 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.el-checkbox-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox-button.is-disabled:first-child .el-checkbox-button__inner{border-left-color:var(--el-button-disabled-border-color,var(--el-border-color-light))}.el-checkbox-button:first-child .el-checkbox-button__inner{border-bottom-left-radius:var(--el-border-radius-base);border-left:var(--el-border);border-top-left-radius:var(--el-border-radius-base);box-shadow:none!important}.el-checkbox-button.is-focus .el-checkbox-button__inner{border-color:var(--el-checkbox-button-checked-border-color)}.el-checkbox-button:last-child .el-checkbox-button__inner{border-bottom-right-radius:var(--el-border-radius-base);border-top-right-radius:var(--el-border-radius-base)}.el-checkbox-button--large 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11px}.el-checkbox-group{font-size:0;line-height:0}.el-checkbox{--el-checkbox-font-size:14px;--el-checkbox-font-weight:var(--el-font-weight-primary);--el-checkbox-text-color:var(--el-text-color-regular);--el-checkbox-input-height:14px;--el-checkbox-input-width:14px;--el-checkbox-border-radius:var(--el-border-radius-small);--el-checkbox-bg-color:var(--el-fill-color-blank);--el-checkbox-input-border:var(--el-border);--el-checkbox-disabled-border-color:var(--el-border-color);--el-checkbox-disabled-input-fill:var(--el-fill-color-light);--el-checkbox-disabled-icon-color:var(--el-text-color-placeholder);--el-checkbox-disabled-checked-input-fill:var(--el-border-color-extra-light);--el-checkbox-disabled-checked-input-border-color:var(--el-border-color);--el-checkbox-disabled-checked-icon-color:var(--el-text-color-placeholder);--el-checkbox-checked-text-color:var(--el-color-primary);--el-checkbox-checked-input-border-color:var(--el-color-primary);--el-checkbox-checked-bg-color:var(--el-color-primary);--el-checkbox-checked-icon-color:var(--el-color-white);--el-checkbox-input-border-color-hover:var(--el-color-primary);align-items:center;color:var(--el-checkbox-text-color);cursor:pointer;display:inline-flex;font-size:var(--el-font-size-base);font-weight:var(--el-checkbox-font-weight);height:var(--el-checkbox-height,32px);margin-right:30px;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-checkbox.is-disabled{cursor:not-allowed}.el-checkbox.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-checkbox.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-checkbox.is-bordered.is-disabled{border-color:var(--el-border-color-lighter)}.el-checkbox.is-bordered.el-checkbox--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__label{font-size:var(--el-font-size-base)}.el-checkbox.is-bordered.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.is-bordered.el-checkbox--small{border-radius:calc(var(--el-border-radius-base) - 1px);padding:0 11px 0 7px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner{height:12px;width:12px}.el-checkbox.is-bordered.el-checkbox--small .el-checkbox__inner:after{height:6px;width:2px}.el-checkbox input:focus-visible+.el-checkbox__inner{border-radius:var(--el-checkbox-border-radius);outline:2px solid var(--el-checkbox-input-border-color-hover);outline-offset:1px}.el-checkbox__input{cursor:pointer;display:inline-flex;outline:none;position:relative;white-space:nowrap}.el-checkbox__input.is-disabled .el-checkbox__inner{background-color:var(--el-checkbox-disabled-input-fill);border-color:var(--el-checkbox-disabled-border-color);cursor:not-allowed}.el-checkbox__input.is-disabled .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-icon-color);cursor:not-allowed}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner{background-color:var(--el-checkbox-disabled-checked-input-fill);border-color:var(--el-checkbox-disabled-checked-input-border-color)}.el-checkbox__input.is-disabled.is-indeterminate .el-checkbox__inner:before{background-color:var(--el-checkbox-disabled-checked-icon-color);border-color:var(--el-checkbox-disabled-checked-icon-color)}.el-checkbox__input.is-disabled+span.el-checkbox__label{color:var(--el-disabled-text-color);cursor:not-allowed}.el-checkbox__input.is-checked .el-checkbox__inner{background-color:var(--el-checkbox-checked-bg-color);border-color:var(--el-checkbox-checked-input-border-color)}.el-checkbox__input.is-checked .el-checkbox__inner:after{border-color:var(--el-checkbox-checked-icon-color);transform:rotate(45deg) scaleY(1)}.el-checkbox__input.is-checked+.el-checkbox__label{color:var(--el-checkbox-checked-text-color)}.el-checkbox__input.is-focus:not(.is-checked) 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cubic-bezier(.71,-.46,.29,1.46);width:var(--el-checkbox-input-width);z-index:var(--el-index-normal)}.el-checkbox__inner:hover{border-color:var(--el-checkbox-input-border-color-hover)}.el-checkbox__inner:after{border:1px solid transparent;border-left:0;border-top:0;box-sizing:content-box;content:"";height:7px;left:4px;position:absolute;top:1px;transform:rotate(45deg) scaleY(0);transform-origin:center;transition:transform .15s ease-in .05s;width:3px}.el-checkbox__original{height:0;margin:0;opacity:0;outline:none;position:absolute;width:0;z-index:-1}.el-checkbox__label{display:inline-block;font-size:var(--el-checkbox-font-size);line-height:1;padding-left:8px}.el-checkbox.el-checkbox--large{height:40px}.el-checkbox.el-checkbox--large .el-checkbox__label{font-size:14px}.el-checkbox.el-checkbox--large .el-checkbox__inner{height:14px;width:14px}.el-checkbox.el-checkbox--small{height:24px}.el-checkbox.el-checkbox--small .el-checkbox__label{font-size:12px}.el-checkbox.el-checkbox--small 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79.1666666667%;max-width:79.1666666667%}.el-col-xs-19.is-guttered{display:block}.el-col-xs-offset-19{margin-left:79.1666666667%}.el-col-xs-pull-19{position:relative;right:79.1666666667%}.el-col-xs-push-19{left:79.1666666667%;position:relative}.el-col-xs-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xs-20.is-guttered{display:block}.el-col-xs-offset-20{margin-left:83.3333333333%}.el-col-xs-pull-20{position:relative;right:83.3333333333%}.el-col-xs-push-20{left:83.3333333333%;position:relative}.el-col-xs-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xs-21.is-guttered{display:block}.el-col-xs-offset-21{margin-left:87.5%}.el-col-xs-pull-21{position:relative;right:87.5%}.el-col-xs-push-21{left:87.5%;position:relative}.el-col-xs-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xs-22.is-guttered{display:block}.el-col-xs-offset-22{margin-left:91.6666666667%}.el-col-xs-pull-22{position:relative;right:91.6666666667%}.el-col-xs-push-22{left:91.6666666667%;position:relative}.el-col-xs-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xs-23.is-guttered{display:block}.el-col-xs-offset-23{margin-left:95.8333333333%}.el-col-xs-pull-23{position:relative;right:95.8333333333%}.el-col-xs-push-23{left:95.8333333333%;position:relative}.el-col-xs-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xs-24.is-guttered{display:block}.el-col-xs-offset-24{margin-left:100%}.el-col-xs-pull-24{position:relative;right:100%}.el-col-xs-push-24{left:100%;position:relative}}@media only screen and (min-width:768px){.el-col-sm-0{display:none;flex:0 0 0%;max-width:0}.el-col-sm-0.is-guttered{display:none}.el-col-sm-offset-0{margin-left:0}.el-col-sm-pull-0{position:relative;right:0}.el-col-sm-push-0{left:0;position:relative}.el-col-sm-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-sm-1,.el-col-sm-1.is-guttered{display:block}.el-col-sm-offset-1{margin-left:4.1666666667%}.el-col-sm-pull-1{position:relative;right:4.1666666667%}.el-col-sm-push-1{left:4.1666666667%;position:relative}.el-col-sm-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-sm-2,.el-col-sm-2.is-guttered{display:block}.el-col-sm-offset-2{margin-left:8.3333333333%}.el-col-sm-pull-2{position:relative;right:8.3333333333%}.el-col-sm-push-2{left:8.3333333333%;position:relative}.el-col-sm-3{flex:0 0 12.5%;max-width:12.5%}.el-col-sm-3,.el-col-sm-3.is-guttered{display:block}.el-col-sm-offset-3{margin-left:12.5%}.el-col-sm-pull-3{position:relative;right:12.5%}.el-col-sm-push-3{left:12.5%;position:relative}.el-col-sm-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-sm-4,.el-col-sm-4.is-guttered{display:block}.el-col-sm-offset-4{margin-left:16.6666666667%}.el-col-sm-pull-4{position:relative;right:16.6666666667%}.el-col-sm-push-4{left:16.6666666667%;position:relative}.el-col-sm-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-sm-5,.el-col-sm-5.is-guttered{display:block}.el-col-sm-offset-5{margin-left:20.8333333333%}.el-col-sm-pull-5{position:relative;right:20.8333333333%}.el-col-sm-push-5{left:20.8333333333%;position:relative}.el-col-sm-6{flex:0 0 25%;max-width:25%}.el-col-sm-6,.el-col-sm-6.is-guttered{display:block}.el-col-sm-offset-6{margin-left:25%}.el-col-sm-pull-6{position:relative;right:25%}.el-col-sm-push-6{left:25%;position:relative}.el-col-sm-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-sm-7,.el-col-sm-7.is-guttered{display:block}.el-col-sm-offset-7{margin-left:29.1666666667%}.el-col-sm-pull-7{position:relative;right:29.1666666667%}.el-col-sm-push-7{left:29.1666666667%;position:relative}.el-col-sm-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-sm-8,.el-col-sm-8.is-guttered{display:block}.el-col-sm-offset-8{margin-left:33.3333333333%}.el-col-sm-pull-8{position:relative;right:33.3333333333%}.el-col-sm-push-8{left:33.3333333333%;position:relative}.el-col-sm-9{flex:0 0 37.5%;max-width:37.5%}.el-col-sm-9,.el-col-sm-9.is-guttered{display:block}.el-col-sm-offset-9{margin-left:37.5%}.el-col-sm-pull-9{position:relative;right:37.5%}.el-col-sm-push-9{left:37.5%;position:relative}.el-col-sm-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-sm-10.is-guttered{display:block}.el-col-sm-offset-10{margin-left:41.6666666667%}.el-col-sm-pull-10{position:relative;right:41.6666666667%}.el-col-sm-push-10{left:41.6666666667%;position:relative}.el-col-sm-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-sm-11.is-guttered{display:block}.el-col-sm-offset-11{margin-left:45.8333333333%}.el-col-sm-pull-11{position:relative;right:45.8333333333%}.el-col-sm-push-11{left:45.8333333333%;position:relative}.el-col-sm-12{display:block;flex:0 0 50%;max-width:50%}.el-col-sm-12.is-guttered{display:block}.el-col-sm-offset-12{margin-left:50%}.el-col-sm-pull-12{position:relative;right:50%}.el-col-sm-push-12{left:50%;position:relative}.el-col-sm-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-sm-13.is-guttered{display:block}.el-col-sm-offset-13{margin-left:54.1666666667%}.el-col-sm-pull-13{position:relative;right:54.1666666667%}.el-col-sm-push-13{left:54.1666666667%;position:relative}.el-col-sm-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-sm-14.is-guttered{display:block}.el-col-sm-offset-14{margin-left:58.3333333333%}.el-col-sm-pull-14{position:relative;right:58.3333333333%}.el-col-sm-push-14{left:58.3333333333%;position:relative}.el-col-sm-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-sm-15.is-guttered{display:block}.el-col-sm-offset-15{margin-left:62.5%}.el-col-sm-pull-15{position:relative;right:62.5%}.el-col-sm-push-15{left:62.5%;position:relative}.el-col-sm-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-sm-16.is-guttered{display:block}.el-col-sm-offset-16{margin-left:66.6666666667%}.el-col-sm-pull-16{position:relative;right:66.6666666667%}.el-col-sm-push-16{left:66.6666666667%;position:relative}.el-col-sm-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-sm-17.is-guttered{display:block}.el-col-sm-offset-17{margin-left:70.8333333333%}.el-col-sm-pull-17{position:relative;right:70.8333333333%}.el-col-sm-push-17{left:70.8333333333%;position:relative}.el-col-sm-18{display:block;flex:0 0 75%;max-width:75%}.el-col-sm-18.is-guttered{display:block}.el-col-sm-offset-18{margin-left:75%}.el-col-sm-pull-18{position:relative;right:75%}.el-col-sm-push-18{left:75%;position:relative}.el-col-sm-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-sm-19.is-guttered{display:block}.el-col-sm-offset-19{margin-left:79.1666666667%}.el-col-sm-pull-19{position:relative;right:79.1666666667%}.el-col-sm-push-19{left:79.1666666667%;position:relative}.el-col-sm-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-sm-20.is-guttered{display:block}.el-col-sm-offset-20{margin-left:83.3333333333%}.el-col-sm-pull-20{position:relative;right:83.3333333333%}.el-col-sm-push-20{left:83.3333333333%;position:relative}.el-col-sm-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-sm-21.is-guttered{display:block}.el-col-sm-offset-21{margin-left:87.5%}.el-col-sm-pull-21{position:relative;right:87.5%}.el-col-sm-push-21{left:87.5%;position:relative}.el-col-sm-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-sm-22.is-guttered{display:block}.el-col-sm-offset-22{margin-left:91.6666666667%}.el-col-sm-pull-22{position:relative;right:91.6666666667%}.el-col-sm-push-22{left:91.6666666667%;position:relative}.el-col-sm-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-sm-23.is-guttered{display:block}.el-col-sm-offset-23{margin-left:95.8333333333%}.el-col-sm-pull-23{position:relative;right:95.8333333333%}.el-col-sm-push-23{left:95.8333333333%;position:relative}.el-col-sm-24{display:block;flex:0 0 100%;max-width:100%}.el-col-sm-24.is-guttered{display:block}.el-col-sm-offset-24{margin-left:100%}.el-col-sm-pull-24{position:relative;right:100%}.el-col-sm-push-24{left:100%;position:relative}}@media only screen and (min-width:992px){.el-col-md-0{display:none;flex:0 0 0%;max-width:0}.el-col-md-0.is-guttered{display:none}.el-col-md-offset-0{margin-left:0}.el-col-md-pull-0{position:relative;right:0}.el-col-md-push-0{left:0;position:relative}.el-col-md-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-md-1,.el-col-md-1.is-guttered{display:block}.el-col-md-offset-1{margin-left:4.1666666667%}.el-col-md-pull-1{position:relative;right:4.1666666667%}.el-col-md-push-1{left:4.1666666667%;position:relative}.el-col-md-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-md-2,.el-col-md-2.is-guttered{display:block}.el-col-md-offset-2{margin-left:8.3333333333%}.el-col-md-pull-2{position:relative;right:8.3333333333%}.el-col-md-push-2{left:8.3333333333%;position:relative}.el-col-md-3{flex:0 0 12.5%;max-width:12.5%}.el-col-md-3,.el-col-md-3.is-guttered{display:block}.el-col-md-offset-3{margin-left:12.5%}.el-col-md-pull-3{position:relative;right:12.5%}.el-col-md-push-3{left:12.5%;position:relative}.el-col-md-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-md-4,.el-col-md-4.is-guttered{display:block}.el-col-md-offset-4{margin-left:16.6666666667%}.el-col-md-pull-4{position:relative;right:16.6666666667%}.el-col-md-push-4{left:16.6666666667%;position:relative}.el-col-md-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-md-5,.el-col-md-5.is-guttered{display:block}.el-col-md-offset-5{margin-left:20.8333333333%}.el-col-md-pull-5{position:relative;right:20.8333333333%}.el-col-md-push-5{left:20.8333333333%;position:relative}.el-col-md-6{flex:0 0 25%;max-width:25%}.el-col-md-6,.el-col-md-6.is-guttered{display:block}.el-col-md-offset-6{margin-left:25%}.el-col-md-pull-6{position:relative;right:25%}.el-col-md-push-6{left:25%;position:relative}.el-col-md-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-md-7,.el-col-md-7.is-guttered{display:block}.el-col-md-offset-7{margin-left:29.1666666667%}.el-col-md-pull-7{position:relative;right:29.1666666667%}.el-col-md-push-7{left:29.1666666667%;position:relative}.el-col-md-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-md-8,.el-col-md-8.is-guttered{display:block}.el-col-md-offset-8{margin-left:33.3333333333%}.el-col-md-pull-8{position:relative;right:33.3333333333%}.el-col-md-push-8{left:33.3333333333%;position:relative}.el-col-md-9{flex:0 0 37.5%;max-width:37.5%}.el-col-md-9,.el-col-md-9.is-guttered{display:block}.el-col-md-offset-9{margin-left:37.5%}.el-col-md-pull-9{position:relative;right:37.5%}.el-col-md-push-9{left:37.5%;position:relative}.el-col-md-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-md-10.is-guttered{display:block}.el-col-md-offset-10{margin-left:41.6666666667%}.el-col-md-pull-10{position:relative;right:41.6666666667%}.el-col-md-push-10{left:41.6666666667%;position:relative}.el-col-md-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-md-11.is-guttered{display:block}.el-col-md-offset-11{margin-left:45.8333333333%}.el-col-md-pull-11{position:relative;right:45.8333333333%}.el-col-md-push-11{left:45.8333333333%;position:relative}.el-col-md-12{display:block;flex:0 0 50%;max-width:50%}.el-col-md-12.is-guttered{display:block}.el-col-md-offset-12{margin-left:50%}.el-col-md-pull-12{position:relative;right:50%}.el-col-md-push-12{left:50%;position:relative}.el-col-md-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-md-13.is-guttered{display:block}.el-col-md-offset-13{margin-left:54.1666666667%}.el-col-md-pull-13{position:relative;right:54.1666666667%}.el-col-md-push-13{left:54.1666666667%;position:relative}.el-col-md-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-md-14.is-guttered{display:block}.el-col-md-offset-14{margin-left:58.3333333333%}.el-col-md-pull-14{position:relative;right:58.3333333333%}.el-col-md-push-14{left:58.3333333333%;position:relative}.el-col-md-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-md-15.is-guttered{display:block}.el-col-md-offset-15{margin-left:62.5%}.el-col-md-pull-15{position:relative;right:62.5%}.el-col-md-push-15{left:62.5%;position:relative}.el-col-md-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-md-16.is-guttered{display:block}.el-col-md-offset-16{margin-left:66.6666666667%}.el-col-md-pull-16{position:relative;right:66.6666666667%}.el-col-md-push-16{left:66.6666666667%;position:relative}.el-col-md-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-md-17.is-guttered{display:block}.el-col-md-offset-17{margin-left:70.8333333333%}.el-col-md-pull-17{position:relative;right:70.8333333333%}.el-col-md-push-17{left:70.8333333333%;position:relative}.el-col-md-18{display:block;flex:0 0 75%;max-width:75%}.el-col-md-18.is-guttered{display:block}.el-col-md-offset-18{margin-left:75%}.el-col-md-pull-18{position:relative;right:75%}.el-col-md-push-18{left:75%;position:relative}.el-col-md-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-md-19.is-guttered{display:block}.el-col-md-offset-19{margin-left:79.1666666667%}.el-col-md-pull-19{position:relative;right:79.1666666667%}.el-col-md-push-19{left:79.1666666667%;position:relative}.el-col-md-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-md-20.is-guttered{display:block}.el-col-md-offset-20{margin-left:83.3333333333%}.el-col-md-pull-20{position:relative;right:83.3333333333%}.el-col-md-push-20{left:83.3333333333%;position:relative}.el-col-md-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-md-21.is-guttered{display:block}.el-col-md-offset-21{margin-left:87.5%}.el-col-md-pull-21{position:relative;right:87.5%}.el-col-md-push-21{left:87.5%;position:relative}.el-col-md-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-md-22.is-guttered{display:block}.el-col-md-offset-22{margin-left:91.6666666667%}.el-col-md-pull-22{position:relative;right:91.6666666667%}.el-col-md-push-22{left:91.6666666667%;position:relative}.el-col-md-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-md-23.is-guttered{display:block}.el-col-md-offset-23{margin-left:95.8333333333%}.el-col-md-pull-23{position:relative;right:95.8333333333%}.el-col-md-push-23{left:95.8333333333%;position:relative}.el-col-md-24{display:block;flex:0 0 100%;max-width:100%}.el-col-md-24.is-guttered{display:block}.el-col-md-offset-24{margin-left:100%}.el-col-md-pull-24{position:relative;right:100%}.el-col-md-push-24{left:100%;position:relative}}@media only screen and (min-width:1200px){.el-col-lg-0{display:none;flex:0 0 0%;max-width:0}.el-col-lg-0.is-guttered{display:none}.el-col-lg-offset-0{margin-left:0}.el-col-lg-pull-0{position:relative;right:0}.el-col-lg-push-0{left:0;position:relative}.el-col-lg-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-lg-1,.el-col-lg-1.is-guttered{display:block}.el-col-lg-offset-1{margin-left:4.1666666667%}.el-col-lg-pull-1{position:relative;right:4.1666666667%}.el-col-lg-push-1{left:4.1666666667%;position:relative}.el-col-lg-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-lg-2,.el-col-lg-2.is-guttered{display:block}.el-col-lg-offset-2{margin-left:8.3333333333%}.el-col-lg-pull-2{position:relative;right:8.3333333333%}.el-col-lg-push-2{left:8.3333333333%;position:relative}.el-col-lg-3{flex:0 0 12.5%;max-width:12.5%}.el-col-lg-3,.el-col-lg-3.is-guttered{display:block}.el-col-lg-offset-3{margin-left:12.5%}.el-col-lg-pull-3{position:relative;right:12.5%}.el-col-lg-push-3{left:12.5%;position:relative}.el-col-lg-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-lg-4,.el-col-lg-4.is-guttered{display:block}.el-col-lg-offset-4{margin-left:16.6666666667%}.el-col-lg-pull-4{position:relative;right:16.6666666667%}.el-col-lg-push-4{left:16.6666666667%;position:relative}.el-col-lg-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-lg-5,.el-col-lg-5.is-guttered{display:block}.el-col-lg-offset-5{margin-left:20.8333333333%}.el-col-lg-pull-5{position:relative;right:20.8333333333%}.el-col-lg-push-5{left:20.8333333333%;position:relative}.el-col-lg-6{flex:0 0 25%;max-width:25%}.el-col-lg-6,.el-col-lg-6.is-guttered{display:block}.el-col-lg-offset-6{margin-left:25%}.el-col-lg-pull-6{position:relative;right:25%}.el-col-lg-push-6{left:25%;position:relative}.el-col-lg-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-lg-7,.el-col-lg-7.is-guttered{display:block}.el-col-lg-offset-7{margin-left:29.1666666667%}.el-col-lg-pull-7{position:relative;right:29.1666666667%}.el-col-lg-push-7{left:29.1666666667%;position:relative}.el-col-lg-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-lg-8,.el-col-lg-8.is-guttered{display:block}.el-col-lg-offset-8{margin-left:33.3333333333%}.el-col-lg-pull-8{position:relative;right:33.3333333333%}.el-col-lg-push-8{left:33.3333333333%;position:relative}.el-col-lg-9{flex:0 0 37.5%;max-width:37.5%}.el-col-lg-9,.el-col-lg-9.is-guttered{display:block}.el-col-lg-offset-9{margin-left:37.5%}.el-col-lg-pull-9{position:relative;right:37.5%}.el-col-lg-push-9{left:37.5%;position:relative}.el-col-lg-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-lg-10.is-guttered{display:block}.el-col-lg-offset-10{margin-left:41.6666666667%}.el-col-lg-pull-10{position:relative;right:41.6666666667%}.el-col-lg-push-10{left:41.6666666667%;position:relative}.el-col-lg-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-lg-11.is-guttered{display:block}.el-col-lg-offset-11{margin-left:45.8333333333%}.el-col-lg-pull-11{position:relative;right:45.8333333333%}.el-col-lg-push-11{left:45.8333333333%;position:relative}.el-col-lg-12{display:block;flex:0 0 50%;max-width:50%}.el-col-lg-12.is-guttered{display:block}.el-col-lg-offset-12{margin-left:50%}.el-col-lg-pull-12{position:relative;right:50%}.el-col-lg-push-12{left:50%;position:relative}.el-col-lg-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-lg-13.is-guttered{display:block}.el-col-lg-offset-13{margin-left:54.1666666667%}.el-col-lg-pull-13{position:relative;right:54.1666666667%}.el-col-lg-push-13{left:54.1666666667%;position:relative}.el-col-lg-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-lg-14.is-guttered{display:block}.el-col-lg-offset-14{margin-left:58.3333333333%}.el-col-lg-pull-14{position:relative;right:58.3333333333%}.el-col-lg-push-14{left:58.3333333333%;position:relative}.el-col-lg-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-lg-15.is-guttered{display:block}.el-col-lg-offset-15{margin-left:62.5%}.el-col-lg-pull-15{position:relative;right:62.5%}.el-col-lg-push-15{left:62.5%;position:relative}.el-col-lg-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-lg-16.is-guttered{display:block}.el-col-lg-offset-16{margin-left:66.6666666667%}.el-col-lg-pull-16{position:relative;right:66.6666666667%}.el-col-lg-push-16{left:66.6666666667%;position:relative}.el-col-lg-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-lg-17.is-guttered{display:block}.el-col-lg-offset-17{margin-left:70.8333333333%}.el-col-lg-pull-17{position:relative;right:70.8333333333%}.el-col-lg-push-17{left:70.8333333333%;position:relative}.el-col-lg-18{display:block;flex:0 0 75%;max-width:75%}.el-col-lg-18.is-guttered{display:block}.el-col-lg-offset-18{margin-left:75%}.el-col-lg-pull-18{position:relative;right:75%}.el-col-lg-push-18{left:75%;position:relative}.el-col-lg-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-lg-19.is-guttered{display:block}.el-col-lg-offset-19{margin-left:79.1666666667%}.el-col-lg-pull-19{position:relative;right:79.1666666667%}.el-col-lg-push-19{left:79.1666666667%;position:relative}.el-col-lg-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-lg-20.is-guttered{display:block}.el-col-lg-offset-20{margin-left:83.3333333333%}.el-col-lg-pull-20{position:relative;right:83.3333333333%}.el-col-lg-push-20{left:83.3333333333%;position:relative}.el-col-lg-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-lg-21.is-guttered{display:block}.el-col-lg-offset-21{margin-left:87.5%}.el-col-lg-pull-21{position:relative;right:87.5%}.el-col-lg-push-21{left:87.5%;position:relative}.el-col-lg-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-lg-22.is-guttered{display:block}.el-col-lg-offset-22{margin-left:91.6666666667%}.el-col-lg-pull-22{position:relative;right:91.6666666667%}.el-col-lg-push-22{left:91.6666666667%;position:relative}.el-col-lg-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-lg-23.is-guttered{display:block}.el-col-lg-offset-23{margin-left:95.8333333333%}.el-col-lg-pull-23{position:relative;right:95.8333333333%}.el-col-lg-push-23{left:95.8333333333%;position:relative}.el-col-lg-24{display:block;flex:0 0 100%;max-width:100%}.el-col-lg-24.is-guttered{display:block}.el-col-lg-offset-24{margin-left:100%}.el-col-lg-pull-24{position:relative;right:100%}.el-col-lg-push-24{left:100%;position:relative}}@media only screen and (min-width:1920px){.el-col-xl-0{display:none;flex:0 0 0%;max-width:0}.el-col-xl-0.is-guttered{display:none}.el-col-xl-offset-0{margin-left:0}.el-col-xl-pull-0{position:relative;right:0}.el-col-xl-push-0{left:0;position:relative}.el-col-xl-1{flex:0 0 4.1666666667%;max-width:4.1666666667%}.el-col-xl-1,.el-col-xl-1.is-guttered{display:block}.el-col-xl-offset-1{margin-left:4.1666666667%}.el-col-xl-pull-1{position:relative;right:4.1666666667%}.el-col-xl-push-1{left:4.1666666667%;position:relative}.el-col-xl-2{flex:0 0 8.3333333333%;max-width:8.3333333333%}.el-col-xl-2,.el-col-xl-2.is-guttered{display:block}.el-col-xl-offset-2{margin-left:8.3333333333%}.el-col-xl-pull-2{position:relative;right:8.3333333333%}.el-col-xl-push-2{left:8.3333333333%;position:relative}.el-col-xl-3{flex:0 0 12.5%;max-width:12.5%}.el-col-xl-3,.el-col-xl-3.is-guttered{display:block}.el-col-xl-offset-3{margin-left:12.5%}.el-col-xl-pull-3{position:relative;right:12.5%}.el-col-xl-push-3{left:12.5%;position:relative}.el-col-xl-4{flex:0 0 16.6666666667%;max-width:16.6666666667%}.el-col-xl-4,.el-col-xl-4.is-guttered{display:block}.el-col-xl-offset-4{margin-left:16.6666666667%}.el-col-xl-pull-4{position:relative;right:16.6666666667%}.el-col-xl-push-4{left:16.6666666667%;position:relative}.el-col-xl-5{flex:0 0 20.8333333333%;max-width:20.8333333333%}.el-col-xl-5,.el-col-xl-5.is-guttered{display:block}.el-col-xl-offset-5{margin-left:20.8333333333%}.el-col-xl-pull-5{position:relative;right:20.8333333333%}.el-col-xl-push-5{left:20.8333333333%;position:relative}.el-col-xl-6{flex:0 0 25%;max-width:25%}.el-col-xl-6,.el-col-xl-6.is-guttered{display:block}.el-col-xl-offset-6{margin-left:25%}.el-col-xl-pull-6{position:relative;right:25%}.el-col-xl-push-6{left:25%;position:relative}.el-col-xl-7{flex:0 0 29.1666666667%;max-width:29.1666666667%}.el-col-xl-7,.el-col-xl-7.is-guttered{display:block}.el-col-xl-offset-7{margin-left:29.1666666667%}.el-col-xl-pull-7{position:relative;right:29.1666666667%}.el-col-xl-push-7{left:29.1666666667%;position:relative}.el-col-xl-8{flex:0 0 33.3333333333%;max-width:33.3333333333%}.el-col-xl-8,.el-col-xl-8.is-guttered{display:block}.el-col-xl-offset-8{margin-left:33.3333333333%}.el-col-xl-pull-8{position:relative;right:33.3333333333%}.el-col-xl-push-8{left:33.3333333333%;position:relative}.el-col-xl-9{flex:0 0 37.5%;max-width:37.5%}.el-col-xl-9,.el-col-xl-9.is-guttered{display:block}.el-col-xl-offset-9{margin-left:37.5%}.el-col-xl-pull-9{position:relative;right:37.5%}.el-col-xl-push-9{left:37.5%;position:relative}.el-col-xl-10{display:block;flex:0 0 41.6666666667%;max-width:41.6666666667%}.el-col-xl-10.is-guttered{display:block}.el-col-xl-offset-10{margin-left:41.6666666667%}.el-col-xl-pull-10{position:relative;right:41.6666666667%}.el-col-xl-push-10{left:41.6666666667%;position:relative}.el-col-xl-11{display:block;flex:0 0 45.8333333333%;max-width:45.8333333333%}.el-col-xl-11.is-guttered{display:block}.el-col-xl-offset-11{margin-left:45.8333333333%}.el-col-xl-pull-11{position:relative;right:45.8333333333%}.el-col-xl-push-11{left:45.8333333333%;position:relative}.el-col-xl-12{display:block;flex:0 0 50%;max-width:50%}.el-col-xl-12.is-guttered{display:block}.el-col-xl-offset-12{margin-left:50%}.el-col-xl-pull-12{position:relative;right:50%}.el-col-xl-push-12{left:50%;position:relative}.el-col-xl-13{display:block;flex:0 0 54.1666666667%;max-width:54.1666666667%}.el-col-xl-13.is-guttered{display:block}.el-col-xl-offset-13{margin-left:54.1666666667%}.el-col-xl-pull-13{position:relative;right:54.1666666667%}.el-col-xl-push-13{left:54.1666666667%;position:relative}.el-col-xl-14{display:block;flex:0 0 58.3333333333%;max-width:58.3333333333%}.el-col-xl-14.is-guttered{display:block}.el-col-xl-offset-14{margin-left:58.3333333333%}.el-col-xl-pull-14{position:relative;right:58.3333333333%}.el-col-xl-push-14{left:58.3333333333%;position:relative}.el-col-xl-15{display:block;flex:0 0 62.5%;max-width:62.5%}.el-col-xl-15.is-guttered{display:block}.el-col-xl-offset-15{margin-left:62.5%}.el-col-xl-pull-15{position:relative;right:62.5%}.el-col-xl-push-15{left:62.5%;position:relative}.el-col-xl-16{display:block;flex:0 0 66.6666666667%;max-width:66.6666666667%}.el-col-xl-16.is-guttered{display:block}.el-col-xl-offset-16{margin-left:66.6666666667%}.el-col-xl-pull-16{position:relative;right:66.6666666667%}.el-col-xl-push-16{left:66.6666666667%;position:relative}.el-col-xl-17{display:block;flex:0 0 70.8333333333%;max-width:70.8333333333%}.el-col-xl-17.is-guttered{display:block}.el-col-xl-offset-17{margin-left:70.8333333333%}.el-col-xl-pull-17{position:relative;right:70.8333333333%}.el-col-xl-push-17{left:70.8333333333%;position:relative}.el-col-xl-18{display:block;flex:0 0 75%;max-width:75%}.el-col-xl-18.is-guttered{display:block}.el-col-xl-offset-18{margin-left:75%}.el-col-xl-pull-18{position:relative;right:75%}.el-col-xl-push-18{left:75%;position:relative}.el-col-xl-19{display:block;flex:0 0 79.1666666667%;max-width:79.1666666667%}.el-col-xl-19.is-guttered{display:block}.el-col-xl-offset-19{margin-left:79.1666666667%}.el-col-xl-pull-19{position:relative;right:79.1666666667%}.el-col-xl-push-19{left:79.1666666667%;position:relative}.el-col-xl-20{display:block;flex:0 0 83.3333333333%;max-width:83.3333333333%}.el-col-xl-20.is-guttered{display:block}.el-col-xl-offset-20{margin-left:83.3333333333%}.el-col-xl-pull-20{position:relative;right:83.3333333333%}.el-col-xl-push-20{left:83.3333333333%;position:relative}.el-col-xl-21{display:block;flex:0 0 87.5%;max-width:87.5%}.el-col-xl-21.is-guttered{display:block}.el-col-xl-offset-21{margin-left:87.5%}.el-col-xl-pull-21{position:relative;right:87.5%}.el-col-xl-push-21{left:87.5%;position:relative}.el-col-xl-22{display:block;flex:0 0 91.6666666667%;max-width:91.6666666667%}.el-col-xl-22.is-guttered{display:block}.el-col-xl-offset-22{margin-left:91.6666666667%}.el-col-xl-pull-22{position:relative;right:91.6666666667%}.el-col-xl-push-22{left:91.6666666667%;position:relative}.el-col-xl-23{display:block;flex:0 0 95.8333333333%;max-width:95.8333333333%}.el-col-xl-23.is-guttered{display:block}.el-col-xl-offset-23{margin-left:95.8333333333%}.el-col-xl-pull-23{position:relative;right:95.8333333333%}.el-col-xl-push-23{left:95.8333333333%;position:relative}.el-col-xl-24{display:block;flex:0 0 100%;max-width:100%}.el-col-xl-24.is-guttered{display:block}.el-col-xl-offset-24{margin-left:100%}.el-col-xl-pull-24{position:relative;right:100%}.el-col-xl-push-24{left:100%;position:relative}}.el-collapse{--el-collapse-border-color:var(--el-border-color-lighter);--el-collapse-header-height:48px;--el-collapse-header-bg-color:var(--el-fill-color-blank);--el-collapse-header-text-color:var(--el-text-color-primary);--el-collapse-header-font-size:13px;--el-collapse-content-bg-color:var(--el-fill-color-blank);--el-collapse-content-font-size:13px;--el-collapse-content-text-color:var(--el-text-color-primary);border-bottom:1px solid var(--el-collapse-border-color);border-top:1px solid var(--el-collapse-border-color)}.el-collapse-item.is-disabled .el-collapse-item__header{color:var(--el-text-color-disabled);cursor:not-allowed}.el-collapse-item__header{align-items:center;background-color:var(--el-collapse-header-bg-color);border:none;border-bottom:1px solid var(--el-collapse-border-color);color:var(--el-collapse-header-text-color);cursor:pointer;display:flex;font-size:var(--el-collapse-header-font-size);font-weight:500;height:var(--el-collapse-header-height);line-height:var(--el-collapse-header-height);outline:none;padding:0;transition:border-bottom-color var(--el-transition-duration);width:100%}.el-collapse-item__arrow{font-weight:300;margin:0 8px 0 auto;transition:transform var(--el-transition-duration)}.el-collapse-item__arrow.is-active{transform:rotate(90deg)}.el-collapse-item__header.focusing:focus:not(:hover){color:var(--el-color-primary)}.el-collapse-item__header.is-active{border-bottom-color:transparent}.el-collapse-item__wrap{background-color:var(--el-collapse-content-bg-color);border-bottom:1px solid var(--el-collapse-border-color);box-sizing:border-box;overflow:hidden;will-change:height}.el-collapse-item__content{color:var(--el-collapse-content-text-color);font-size:var(--el-collapse-content-font-size);line-height:1.7692307692;padding-bottom:25px}.el-collapse-item:last-child{margin-bottom:-1px}.el-color-predefine{display:flex;font-size:12px;margin-top:8px;width:280px}.el-color-predefine__colors{display:flex;flex:1;flex-wrap:wrap}.el-color-predefine__color-selector{border-radius:4px;cursor:pointer;height:20px;margin:0 0 8px 8px;width:20px}.el-color-predefine__color-selector:nth-child(10n+1){margin-left:0}.el-color-predefine__color-selector.selected{box-shadow:0 0 3px 2px var(--el-color-primary)}.el-color-predefine__color-selector>div{border-radius:3px;display:flex;height:100%}.el-color-predefine__color-selector.is-alpha{background-image:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAwAAAAMCAIAAADZF8uwAAAAGUlEQVQYV2M4gwH+YwCGIasIUwhT25BVBADtzYNYrHvv4gAAAABJRU5ErkJggg==)}.el-color-hue-slider{background-color:red;box-sizing:border-box;float:right;height:12px;padding:0 2px;position:relative;width:280px}.el-color-hue-slider__bar{background:linear-gradient(90deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red);height:100%;position:relative}.el-color-hue-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-hue-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-hue-slider.is-vertical{height:180px;padding:2px 0;width:12px}.el-color-hue-slider.is-vertical .el-color-hue-slider__bar{background:linear-gradient(180deg,red 0,#ff0 17%,#0f0 33%,#0ff 50%,#00f 67%,#f0f 83%,red)}.el-color-hue-slider.is-vertical .el-color-hue-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-svpanel{height:180px;position:relative;width:280px}.el-color-svpanel__black,.el-color-svpanel__white{bottom:0;left:0;position:absolute;right:0;top:0}.el-color-svpanel__white{background:linear-gradient(90deg,#fff,hsla(0,0%,100%,0))}.el-color-svpanel__black{background:linear-gradient(0deg,#000,transparent)}.el-color-svpanel__cursor{position:absolute}.el-color-svpanel__cursor>div{border-radius:50%;box-shadow:0 0 0 1.5px #fff,inset 0 0 1px 1px rgba(0,0,0,.3),0 0 1px 2px rgba(0,0,0,.4);cursor:head;height:4px;transform:translate(-2px,-2px);width:4px}.el-color-alpha-slider{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px;box-sizing:border-box;height:12px;position:relative;width:280px}.el-color-alpha-slider__bar{background:linear-gradient(to right,hsla(0,0%,100%,0) 0,var(--el-bg-color) 100%);height:100%;position:relative}.el-color-alpha-slider__thumb{background:#fff;border:1px solid var(--el-border-color-lighter);border-radius:1px;box-shadow:0 0 2px rgba(0,0,0,.6);box-sizing:border-box;cursor:pointer;height:100%;left:0;position:absolute;top:0;width:4px;z-index:1}.el-color-alpha-slider__thumb:focus-visible{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-alpha-slider.is-vertical{height:180px;width:20px}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__bar{background:linear-gradient(180deg,hsla(0,0%,100%,0) 0,#fff)}.el-color-alpha-slider.is-vertical .el-color-alpha-slider__thumb{height:4px;left:0;top:0;width:100%}.el-color-dropdown{width:300px}.el-color-dropdown__main-wrapper{margin-bottom:6px}.el-color-dropdown__main-wrapper:after{clear:both;content:"";display:table}.el-color-dropdown__btns{margin-top:12px;text-align:right}.el-color-dropdown__value{color:#000;float:left;font-size:12px;line-height:26px;width:160px}.el-color-picker{display:inline-block;line-height:normal;outline:none;position:relative}.el-color-picker:hover:not(.is-disabled,.is-focused) .el-color-picker__trigger{border-color:var(--el-border-color-hover)}.el-color-picker:focus-visible:not(.is-disabled) .el-color-picker__trigger{outline:2px solid var(--el-color-primary);outline-offset:1px}.el-color-picker.is-focused .el-color-picker__trigger{border-color:var(--el-color-primary)}.el-color-picker.is-disabled .el-color-picker__trigger{cursor:not-allowed}.el-color-picker--large{height:40px}.el-color-picker--large .el-color-picker__trigger{height:40px;width:40px}.el-color-picker--large .el-color-picker__mask{height:38px;width:38px}.el-color-picker--small{height:24px}.el-color-picker--small .el-color-picker__trigger{height:24px;width:24px}.el-color-picker--small .el-color-picker__mask{height:22px;width:22px}.el-color-picker--small .el-color-picker__empty,.el-color-picker--small .el-color-picker__icon{transform:scale(.8)}.el-color-picker__mask{background-color:hsla(0,0%,100%,.7);border-radius:4px;cursor:not-allowed;height:30px;left:1px;position:absolute;top:1px;width:30px;z-index:1}.el-color-picker__trigger{align-items:center;border:1px solid var(--el-border-color);border-radius:4px;box-sizing:border-box;cursor:pointer;display:inline-flex;font-size:0;height:32px;justify-content:center;padding:4px;position:relative;width:32px}.el-color-picker__color{border:1px solid var(--el-text-color-secondary);border-radius:var(--el-border-radius-small);box-sizing:border-box;display:block;height:100%;position:relative;text-align:center;width:100%}.el-color-picker__color.is-alpha{background-image:linear-gradient(45deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-a) 25%,var(--el-color-picker-alpha-bg-b) 25%),linear-gradient(45deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%),linear-gradient(135deg,var(--el-color-picker-alpha-bg-b) 75%,var(--el-color-picker-alpha-bg-a) 75%);background-position:0 0,6px 0,6px -6px,0 6px;background-size:12px 12px}.el-color-picker__color-inner{align-items:center;display:inline-flex;height:100%;justify-content:center;width:100%}.el-color-picker .el-color-picker__empty{color:var(--el-text-color-secondary);font-size:12px}.el-color-picker .el-color-picker__icon{align-items:center;color:#fff;display:inline-flex;font-size:12px;justify-content:center}.el-color-picker__panel{background-color:#fff;border-radius:var(--el-border-radius-base);box-shadow:var(--el-box-shadow-light);box-sizing:content-box;padding:6px;position:absolute;z-index:10}.el-color-picker__panel.el-popper{border:1px solid var(--el-border-color-lighter)}.el-color-picker,.el-color-picker__panel{--el-color-picker-alpha-bg-a:#ccc;--el-color-picker-alpha-bg-b:transparent}.dark .el-color-picker,.dark .el-color-picker__panel{--el-color-picker-alpha-bg-a:#333}.el-container{box-sizing:border-box;display:flex;flex:1;flex-basis:auto;flex-direction:row;min-width:0}.el-container.is-vertical{flex-direction:column}.el-date-table{font-size:12px;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-date-table.is-week-mode .el-date-table__row:hover .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table.is-week-mode .el-date-table__row:hover td.available:hover{color:var(--el-datepicker-text-color)}.el-date-table.is-week-mode .el-date-table__row:hover td:first-child .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table.is-week-mode .el-date-table__row:hover td:last-child .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table.is-week-mode .el-date-table__row.current .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td{box-sizing:border-box;cursor:pointer;height:30px;padding:4px 0;position:relative;text-align:center;width:32px}.el-date-table td .el-date-table-cell{box-sizing:border-box;height:30px;padding:3px 0}.el-date-table td .el-date-table-cell .el-date-table-cell__text{border-radius:50%;display:block;height:24px;left:50%;line-height:24px;margin:0 auto;position:absolute;transform:translateX(-50%);width:24px}.el-date-table td.next-month,.el-date-table td.prev-month{color:var(--el-datepicker-off-text-color)}.el-date-table td.today{position:relative}.el-date-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-date-table td.today.end-date .el-date-table-cell__text,.el-date-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-date-table td.available:hover{color:var(--el-datepicker-hover-text-color)}.el-date-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-date-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-date-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-date-table td.current:not(.disabled):focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-date-table td.end-date .el-date-table-cell,.el-date-table td.start-date .el-date-table-cell{color:#fff}.el-date-table td.end-date .el-date-table-cell__text,.el-date-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color)}.el-date-table td.start-date .el-date-table-cell{border-bottom-left-radius:15px;border-top-left-radius:15px;margin-left:5px}.el-date-table td.end-date .el-date-table-cell{border-bottom-right-radius:15px;border-top-right-radius:15px;margin-right:5px}.el-date-table td.disabled .el-date-table-cell{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed;opacity:1}.el-date-table td.selected .el-date-table-cell{border-radius:15px;margin-left:5px;margin-right:5px}.el-date-table td.selected .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);border-radius:15px;color:#fff}.el-date-table td.week{color:var(--el-datepicker-header-text-color);font-size:80%}.el-date-table td:focus{outline:none}.el-date-table th{border-bottom:1px solid var(--el-border-color-lighter);color:var(--el-datepicker-header-text-color);font-weight:400;padding:5px}.el-month-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-month-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-month-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-month-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-month-table td.today.end-date .el-date-table-cell__text,.el-month-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-month-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-month-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-month-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:54px}.el-month-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-month-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-month-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-month-table td.end-date .el-date-table-cell,.el-month-table td.start-date .el-date-table-cell{color:#fff}.el-month-table td.end-date .el-date-table-cell__text,.el-month-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px;margin-left:3px}.el-month-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell{border-radius:24px;margin-left:3px;margin-right:3px}.el-month-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-month-table td:focus-visible{outline:none}.el-month-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-year-table{border-collapse:collapse;font-size:12px;margin:-1px}.el-year-table .el-icon{color:var(--el-datepicker-icon-color)}.el-year-table td{cursor:pointer;padding:8px 0;position:relative;text-align:center;width:68px}.el-year-table td .el-date-table-cell{box-sizing:border-box;height:48px;padding:6px 0}.el-year-table td.today .el-date-table-cell__text{color:var(--el-color-primary);font-weight:700}.el-year-table td.today.end-date .el-date-table-cell__text,.el-year-table td.today.start-date .el-date-table-cell__text{color:#fff}.el-year-table td.disabled .el-date-table-cell__text{background-color:var(--el-fill-color-light);color:var(--el-text-color-placeholder);cursor:not-allowed}.el-year-table td.disabled .el-date-table-cell__text:hover{color:var(--el-text-color-placeholder)}.el-year-table td .el-date-table-cell__text{border-radius:18px;color:var(--el-datepicker-text-color);display:block;height:36px;left:50%;line-height:36px;margin:0 auto;position:absolute;transform:translateX(-50%);width:60px}.el-year-table td .el-date-table-cell__text:hover{color:var(--el-datepicker-hover-text-color)}.el-year-table td.in-range .el-date-table-cell{background-color:var(--el-datepicker-inrange-bg-color)}.el-year-table td.in-range .el-date-table-cell:hover{background-color:var(--el-datepicker-inrange-hover-bg-color)}.el-year-table td.end-date .el-date-table-cell,.el-year-table td.start-date .el-date-table-cell{color:#fff}.el-year-table td.end-date .el-date-table-cell__text,.el-year-table td.start-date .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td.start-date .el-date-table-cell{border-bottom-left-radius:24px;border-top-left-radius:24px}.el-year-table td.end-date .el-date-table-cell{border-bottom-right-radius:24px;border-top-right-radius:24px}.el-year-table td.current:not(.disabled) .el-date-table-cell__text{background-color:var(--el-datepicker-active-color);color:#fff}.el-year-table td:focus-visible{outline:none}.el-year-table td:focus-visible .el-date-table-cell__text{outline:2px solid var(--el-datepicker-active-color);outline-offset:1px}.el-time-spinner.has-seconds .el-time-spinner__wrapper{width:33.3%}.el-time-spinner__wrapper{display:inline-block;max-height:192px;overflow:auto;position:relative;vertical-align:top;width:50%}.el-time-spinner__wrapper.el-scrollbar__wrap:not(.el-scrollbar__wrap--hidden-default){padding-bottom:15px}.el-time-spinner__wrapper.is-arrow{box-sizing:border-box;overflow:hidden;text-align:center}.el-time-spinner__wrapper.is-arrow .el-time-spinner__list{transform:translateY(-32px)}.el-time-spinner__wrapper.is-arrow .el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:default}.el-time-spinner__arrow{color:var(--el-text-color-secondary);cursor:pointer;font-size:12px;height:30px;left:0;line-height:30px;position:absolute;text-align:center;width:100%;z-index:var(--el-index-normal)}.el-time-spinner__arrow:hover{color:var(--el-color-primary)}.el-time-spinner__arrow.arrow-up{top:10px}.el-time-spinner__arrow.arrow-down{bottom:10px}.el-time-spinner__input.el-input{width:70%}.el-time-spinner__input.el-input .el-input__inner,.el-time-spinner__list{padding:0;text-align:center}.el-time-spinner__list{list-style:none;margin:0}.el-time-spinner__list:after,.el-time-spinner__list:before{content:"";display:block;height:80px;width:100%}.el-time-spinner__item{color:var(--el-text-color-regular);font-size:12px;height:32px;line-height:32px}.el-time-spinner__item:hover:not(.is-disabled):not(.is-active){background:var(--el-fill-color-light);cursor:pointer}.el-time-spinner__item.is-active:not(.is-disabled){color:var(--el-text-color-primary);font-weight:700}.el-time-spinner__item.is-disabled{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-picker__popper{--el-datepicker-border-color:var(--el-disabled-border-color)}.el-picker__popper.el-popper{background:var(--el-bg-color-overlay);box-shadow:var(--el-box-shadow-light)}.el-picker__popper.el-popper,.el-picker__popper.el-popper .el-popper__arrow:before{border:1px solid var(--el-datepicker-border-color)}.el-picker__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-picker__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-date-editor{--el-date-editor-width:220px;--el-date-editor-monthrange-width:300px;--el-date-editor-daterange-width:350px;--el-date-editor-datetimerange-width:400px;--el-input-text-color:var(--el-text-color-regular);--el-input-border:var(--el-border);--el-input-hover-border:var(--el-border-color-hover);--el-input-focus-border:var(--el-color-primary);--el-input-transparent-border:0 0 0 1px transparent inset;--el-input-border-color:var(--el-border-color);--el-input-border-radius:var(--el-border-radius-base);--el-input-bg-color:var(--el-fill-color-blank);--el-input-icon-color:var(--el-text-color-placeholder);--el-input-placeholder-color:var(--el-text-color-placeholder);--el-input-hover-border-color:var(--el-border-color-hover);--el-input-clear-hover-color:var(--el-text-color-secondary);--el-input-focus-border-color:var(--el-color-primary);--el-input-width:100%;position:relative;text-align:left;vertical-align:middle}.el-date-editor.el-input__wrapper{box-shadow:0 0 0 1px var(--el-input-border-color,var(--el-border-color)) inset}.el-date-editor.el-input__wrapper:hover{box-shadow:0 0 0 1px var(--el-input-hover-border-color) inset}.el-date-editor.el-input,.el-date-editor.el-input__wrapper{height:var(--el-input-height,var(--el-component-size));width:var(--el-date-editor-width)}.el-date-editor--monthrange{--el-date-editor-width:var(--el-date-editor-monthrange-width)}.el-date-editor--daterange,.el-date-editor--timerange{--el-date-editor-width:var(--el-date-editor-daterange-width)}.el-date-editor--datetimerange{--el-date-editor-width:var(--el-date-editor-datetimerange-width)}.el-date-editor--dates .el-input__wrapper{text-overflow:ellipsis;white-space:nowrap}.el-date-editor .clear-icon,.el-date-editor .close-icon{cursor:pointer}.el-date-editor .clear-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__icon{color:var(--el-text-color-placeholder);float:left;font-size:14px;height:inherit}.el-date-editor .el-range__icon svg{vertical-align:middle}.el-date-editor .el-range-input{-webkit-appearance:none;-moz-appearance:none;appearance:none;background-color:transparent;border:none;color:var(--el-text-color-regular);display:inline-block;font-size:var(--el-font-size-base);height:30px;line-height:30px;margin:0;outline:none;padding:0;text-align:center;width:39%}.el-date-editor .el-range-input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-input::placeholder{color:var(--el-text-color-placeholder)}.el-date-editor .el-range-separator{align-items:center;color:var(--el-text-color-primary);display:inline-flex;flex:1;font-size:14px;height:100%;justify-content:center;margin:0;overflow-wrap:break-word;padding:0 5px}.el-date-editor .el-range__close-icon{color:var(--el-text-color-placeholder);cursor:pointer;font-size:14px;height:inherit;width:unset}.el-date-editor .el-range__close-icon:hover{color:var(--el-text-color-secondary)}.el-date-editor .el-range__close-icon svg{vertical-align:middle}.el-date-editor .el-range__close-icon--hidden{opacity:0;visibility:hidden}.el-range-editor.el-input__wrapper{align-items:center;display:inline-flex;padding:0 10px;vertical-align:middle}.el-range-editor.is-active,.el-range-editor.is-active:hover{box-shadow:0 0 0 1px var(--el-input-focus-border-color) inset}.el-range-editor--large{line-height:var(--el-component-size-large)}.el-range-editor--large.el-input__wrapper{height:var(--el-component-size-large)}.el-range-editor--large .el-range-separator{font-size:14px;line-height:40px}.el-range-editor--large .el-range-input{font-size:14px;height:38px;line-height:38px}.el-range-editor--small{line-height:var(--el-component-size-small)}.el-range-editor--small.el-input__wrapper{height:var(--el-component-size-small)}.el-range-editor--small .el-range-separator{font-size:12px;line-height:24px}.el-range-editor--small .el-range-input{font-size:12px;height:22px;line-height:22px}.el-range-editor.is-disabled{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled,.el-range-editor.is-disabled:focus,.el-range-editor.is-disabled:hover{border-color:var(--el-disabled-border-color)}.el-range-editor.is-disabled input{background-color:var(--el-disabled-bg-color);color:var(--el-disabled-text-color);cursor:not-allowed}.el-range-editor.is-disabled input::-moz-placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled input::placeholder{color:var(--el-text-color-placeholder)}.el-range-editor.is-disabled .el-range-separator{color:var(--el-disabled-text-color)}.el-picker-panel{background:var(--el-bg-color-overlay);border-radius:var(--el-border-radius-base);color:var(--el-text-color-regular);line-height:30px}.el-picker-panel .el-time-panel{background-color:var(--el-bg-color-overlay);border:1px solid var(--el-datepicker-border-color);box-shadow:var(--el-box-shadow-light);margin:5px 0}.el-picker-panel__body-wrapper:after,.el-picker-panel__body:after{clear:both;content:"";display:table}.el-picker-panel__content{margin:15px;position:relative}.el-picker-panel__footer{background-color:var(--el-bg-color-overlay);border-top:1px solid var(--el-datepicker-inner-border-color);font-size:0;padding:4px 12px;position:relative;text-align:right}.el-picker-panel__shortcut{background-color:transparent;border:0;color:var(--el-datepicker-text-color);cursor:pointer;display:block;font-size:14px;line-height:28px;outline:none;padding-left:12px;text-align:left;width:100%}.el-picker-panel__shortcut:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__shortcut.active{background-color:#e6f1fe;color:var(--el-datepicker-active-color)}.el-picker-panel__btn{background-color:transparent;border:1px solid var(--el-fill-color-darker);border-radius:2px;color:var(--el-text-color-primary);cursor:pointer;font-size:12px;line-height:24px;outline:none;padding:0 20px}.el-picker-panel__btn[disabled]{color:var(--el-text-color-disabled);cursor:not-allowed}.el-picker-panel__icon-btn{background:transparent;border:0;color:var(--el-datepicker-icon-color);cursor:pointer;font-size:12px;margin-top:8px;outline:none}.el-picker-panel__icon-btn:hover{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn:focus-visible{color:var(--el-datepicker-hover-text-color)}.el-picker-panel__icon-btn.is-disabled{color:var(--el-text-color-disabled)}.el-picker-panel__icon-btn.is-disabled:hover{cursor:not-allowed}.el-picker-panel__icon-btn .el-icon{cursor:pointer;font-size:inherit}.el-picker-panel__link-btn{vertical-align:middle}.el-picker-panel [slot=sidebar],.el-picker-panel__sidebar{background-color:var(--el-bg-color-overlay);border-right:1px solid var(--el-datepicker-inner-border-color);bottom:0;box-sizing:border-box;overflow:auto;padding-top:6px;position:absolute;top:0;width:110px}.el-picker-panel [slot=sidebar]+.el-picker-panel__body,.el-picker-panel__sidebar+.el-picker-panel__body{margin-left:110px}.el-date-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:322px}.el-date-picker.has-sidebar.has-time{width:434px}.el-date-picker.has-sidebar{width:438px}.el-date-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-picker .el-picker-panel__content{width:292px}.el-date-picker table{table-layout:fixed;width:100%}.el-date-picker__editor-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-picker__time-header{border-bottom:1px solid var(--el-datepicker-inner-border-color);box-sizing:border-box;display:table;font-size:12px;padding:8px 5px 5px;position:relative;width:100%}.el-date-picker__header{padding:12px 12px 0;text-align:center}.el-date-picker__header--bordered{border-bottom:1px solid var(--el-border-color-lighter);margin-bottom:0;padding-bottom:12px}.el-date-picker__header--bordered+.el-picker-panel__content{margin-top:0}.el-date-picker__header-label{color:var(--el-text-color-regular);cursor:pointer;font-size:16px;font-weight:500;line-height:22px;padding:0 5px;text-align:center}.el-date-picker__header-label:hover{color:var(--el-datepicker-hover-text-color)}.el-date-picker__header-label:focus-visible{color:var(--el-datepicker-hover-text-color);outline:none}.el-date-picker__header-label.active{color:var(--el-datepicker-active-color)}.el-date-picker__prev-btn{float:left}.el-date-picker__next-btn{float:right}.el-date-picker__time-wrap{padding:10px;text-align:center}.el-date-picker__time-label{cursor:pointer;float:left;line-height:30px;margin-left:10px}.el-date-picker .el-time-panel{position:absolute}.el-date-range-picker{--el-datepicker-text-color:var(--el-text-color-regular);--el-datepicker-off-text-color:var(--el-text-color-placeholder);--el-datepicker-header-text-color:var(--el-text-color-regular);--el-datepicker-icon-color:var(--el-text-color-primary);--el-datepicker-border-color:var(--el-disabled-border-color);--el-datepicker-inner-border-color:var(--el-border-color-light);--el-datepicker-inrange-bg-color:var(--el-border-color-extra-light);--el-datepicker-inrange-hover-bg-color:var(--el-border-color-extra-light);--el-datepicker-active-color:var(--el-color-primary);--el-datepicker-hover-text-color:var(--el-color-primary);width:646px}.el-date-range-picker.has-sidebar{width:756px}.el-date-range-picker.has-time .el-picker-panel__body-wrapper{position:relative}.el-date-range-picker table{table-layout:fixed;width:100%}.el-date-range-picker .el-picker-panel__body{min-width:513px}.el-date-range-picker 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5px;position:relative;width:100%}.el-date-range-picker__time-header>.el-icon-arrow-right{color:var(--el-datepicker-icon-color);display:table-cell;font-size:20px;vertical-align:middle}.el-date-range-picker__time-picker-wrap{display:table-cell;padding:0 5px;position:relative}.el-date-range-picker__time-picker-wrap .el-picker-panel{background:#fff;position:absolute;right:0;top:13px;z-index:1}.el-date-range-picker__time-picker-wrap .el-time-panel{position:absolute}.el-time-range-picker{overflow:visible;width:354px}.el-time-range-picker__content{padding:10px;position:relative;text-align:center;z-index:1}.el-time-range-picker__cell{box-sizing:border-box;display:inline-block;margin:0;padding:4px 7px 7px;width:50%}.el-time-range-picker__header{font-size:14px;margin-bottom:5px;text-align:center}.el-time-range-picker__body{border:1px solid var(--el-datepicker-border-color);border-radius:2px}.el-time-panel{border-radius:2px;box-sizing:content-box;left:0;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;width:180px;z-index:var(--el-index-top)}.el-time-panel__content{font-size:0;overflow:hidden;position:relative}.el-time-panel__content:after,.el-time-panel__content:before{box-sizing:border-box;content:"";height:32px;left:0;margin-top:-16px;padding-top:6px;position:absolute;right:0;text-align:left;top:50%;z-index:-1}.el-time-panel__content:after{left:50%;margin-left:12%;margin-right:12%}.el-time-panel__content:before{border-bottom:1px solid var(--el-border-color-light);border-top:1px solid var(--el-border-color-light);margin-left:12%;margin-right:12%;padding-left:50%}.el-time-panel__content.has-seconds:after{left:66.6666666667%}.el-time-panel__content.has-seconds:before{padding-left:33.3333333333%}.el-time-panel__footer{border-top:1px solid 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var(--el-transition-duration)}.dialog-fade-leave-active .el-overlay-dialog{animation:dialog-fade-out var(--el-transition-duration)}@keyframes dialog-fade-in{0%{opacity:0;transform:translate3d(0,-20px,0)}to{opacity:1;transform:translateZ(0)}}@keyframes dialog-fade-out{0%{opacity:1;transform:translateZ(0)}to{opacity:0;transform:translate3d(0,-20px,0)}}@keyframes modal-fade-in{0%{opacity:0}to{opacity:1}}@keyframes modal-fade-out{0%{opacity:1}to{opacity:0}}.el-divider{position:relative}.el-divider--horizontal{border-top:1px var(--el-border-color) var(--el-border-style);display:block;height:1px;margin:24px 0;width:100%}.el-divider--vertical{border-left:1px var(--el-border-color) var(--el-border-style);display:inline-block;height:1em;margin:0 8px;position:relative;vertical-align:middle;width:1px}.el-divider__text{background-color:var(--el-bg-color);color:var(--el-text-color-primary);font-size:14px;font-weight:500;padding:0 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.ttb{transform:translate(0)}.el-drawer__sr-focus:focus{outline:none!important}.el-drawer__header{align-items:center;color:#72767b;display:flex;margin-bottom:32px;padding:var(--el-drawer-padding-primary);padding-bottom:0}.el-drawer__header>:first-child{flex:1}.el-drawer__title{flex:1;font-size:16px;line-height:inherit;margin:0}.el-drawer__footer{padding:var(--el-drawer-padding-primary);padding-top:10px;text-align:right}.el-drawer__close-btn{background-color:transparent;border:none;color:inherit;cursor:pointer;display:inline-flex;font-size:var(--el-font-size-extra-large);outline:none}.el-drawer__close-btn:focus i,.el-drawer__close-btn:hover 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.el-popper__arrow:before{border:1px solid var(--el-border-color-light)}.el-dropdown__popper.el-popper[data-popper-placement^=top] .el-popper__arrow:before{border-left-color:transparent;border-top-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=bottom] .el-popper__arrow:before{border-bottom-color:transparent;border-right-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=left] .el-popper__arrow:before{border-bottom-color:transparent;border-left-color:transparent}.el-dropdown__popper.el-popper[data-popper-placement^=right] .el-popper__arrow:before{border-right-color:transparent;border-top-color:transparent}.el-dropdown__popper .el-dropdown-menu{border:none}.el-dropdown__popper .el-dropdown__popper-selfdefine{outline:none}.el-dropdown__popper .el-scrollbar__bar{z-index:calc(var(--el-dropdown-menu-index) + 1)}.el-dropdown__popper .el-dropdown__list{box-sizing:border-box;list-style:none;margin:0;padding:0}.el-dropdown 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var(--el-transition-duration);width:var(--el-notification-width);z-index:9999}.el-notification.right{right:16px}.el-notification.left{left:16px}.el-notification__group{margin-left:var(--el-notification-group-margin-left);margin-right:var(--el-notification-group-margin-right)}.el-notification__title{color:var(--el-notification-title-color);font-size:var(--el-notification-title-font-size);font-weight:700;line-height:var(--el-notification-icon-size);margin:0}.el-notification__content{color:var(--el-notification-content-color);font-size:var(--el-notification-content-font-size);line-height:24px;margin:6px 0 0}.el-notification__content p{margin:0}.el-notification .el-notification__icon{font-size:var(--el-notification-icon-size);height:var(--el-notification-icon-size);width:var(--el-notification-icon-size)}.el-notification .el-notification__closeBtn{color:var(--el-notification-close-color);cursor:pointer;font-size:var(--el-notification-close-font-size);position:absolute;right:15px;top:18px}.el-notification .el-notification__closeBtn:hover{color:var(--el-notification-close-hover-color)}.el-notification .el-notification--success{--el-notification-icon-color:var(--el-color-success);color:var(--el-notification-icon-color)}.el-notification .el-notification--info{--el-notification-icon-color:var(--el-color-info);color:var(--el-notification-icon-color)}.el-notification .el-notification--warning{--el-notification-icon-color:var(--el-color-warning);color:var(--el-notification-icon-color)}.el-notification .el-notification--error{--el-notification-icon-color:var(--el-color-error);color:var(--el-notification-icon-color)}.el-notification-fade-enter-from.right{right:0;transform:translateX(100%)}.el-notification-fade-enter-from.left{left:0;transform:translateX(-100%)}.el-notification-fade-leave-to{opacity:0}.el-overlay{background-color:var(--el-overlay-color-lighter);bottom:0;height:100%;left:0;overflow:auto;position:fixed;right:0;top:0;z-index:2000}.el-overlay .el-overlay-root{height:0}.el-page-header.is-contentful .el-page-header__main{border-top:1px solid var(--el-border-color-light);margin-top:16px}.el-page-header__header{align-items:center;display:flex;justify-content:space-between;line-height:24px}.el-page-header__left{align-items:center;display:flex;margin-right:40px;position:relative}.el-page-header__back{align-items:center;cursor:pointer;display:flex}.el-page-header__left .el-divider--vertical{margin:0 16px}.el-page-header__icon{align-items:center;display:flex;font-size:16px;margin-right:10px}.el-page-header__icon .el-icon{font-size:inherit}.el-page-header__title{font-size:14px;font-weight:500}.el-page-header__content{color:var(--el-text-color-primary);font-size:18px}.el-page-header__breadcrumb{margin-bottom:16px}.el-pagination{--el-pagination-font-size:14px;--el-pagination-bg-color:var(--el-fill-color-blank);--el-pagination-text-color:var(--el-text-color-primary);--el-pagination-border-radius:2px;--el-pagination-button-color:var(--el-text-color-primary);--el-pagination-button-width:32px;--el-pagination-button-height:32px;--el-pagination-button-disabled-color:var(--el-text-color-placeholder);--el-pagination-button-disabled-bg-color:var(--el-fill-color-blank);--el-pagination-button-bg-color:var(--el-fill-color);--el-pagination-hover-color:var(--el-color-primary);--el-pagination-font-size-small:12px;--el-pagination-button-width-small:24px;--el-pagination-button-height-small:24px;--el-pagination-button-width-large:40px;--el-pagination-button-height-large:40px;--el-pagination-item-gap:16px;align-items:center;color:var(--el-pagination-text-color);display:flex;font-size:var(--el-pagination-font-size);font-weight:400;white-space:nowrap}.el-pagination .el-input__inner{-moz-appearance:textfield;text-align:center}.el-pagination .el-select{width:128px}.el-pagination button{align-items:center;background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;display:flex;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pagination button *{pointer-events:none}.el-pagination button:focus{outline:none}.el-pagination button.is-active,.el-pagination button:hover{color:var(--el-pagination-hover-color)}.el-pagination button.is-active{cursor:default;font-weight:700}.el-pagination button.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pagination button.is-disabled,.el-pagination button:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pagination button:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-pagination .btn-next .el-icon,.el-pagination .btn-prev .el-icon{display:block;font-size:12px;font-weight:700;width:inherit}.el-pagination>.is-first{margin-left:0!important}.el-pagination>.is-last{margin-right:0!important}.el-pagination .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination__sizes,.el-pagination__total{color:var(--el-text-color-regular);font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__total[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__jump{align-items:center;color:var(--el-text-color-regular);display:flex;font-weight:400;margin-left:var(--el-pagination-item-gap)}.el-pagination__jump[disabled=true]{color:var(--el-text-color-placeholder)}.el-pagination__goto{margin-right:8px}.el-pagination__editor{box-sizing:border-box;text-align:center}.el-pagination__editor.el-input{width:56px}.el-pagination__editor .el-input__inner::-webkit-inner-spin-button,.el-pagination__editor .el-input__inner::-webkit-outer-spin-button{-webkit-appearance:none;margin:0}.el-pagination__classifier{margin-left:8px}.el-pagination__rightwrapper{align-items:center;display:flex;flex:1;justify-content:flex-end}.el-pagination.is-background .btn-next,.el-pagination.is-background .btn-prev,.el-pagination.is-background .el-pager li{background-color:var(--el-pagination-button-bg-color);margin:0 4px}.el-pagination.is-background .btn-next.is-active,.el-pagination.is-background .btn-prev.is-active,.el-pagination.is-background .el-pager li.is-active{background-color:var(--el-color-primary);color:var(--el-color-white)}.el-pagination.is-background .btn-next.is-disabled,.el-pagination.is-background .btn-next:disabled,.el-pagination.is-background .btn-prev.is-disabled,.el-pagination.is-background .btn-prev:disabled,.el-pagination.is-background .el-pager li.is-disabled,.el-pagination.is-background .el-pager li:disabled{background-color:var(--el-disabled-bg-color);color:var(--el-text-color-placeholder)}.el-pagination.is-background .btn-next.is-disabled.is-active,.el-pagination.is-background .btn-next:disabled.is-active,.el-pagination.is-background .btn-prev.is-disabled.is-active,.el-pagination.is-background .btn-prev:disabled.is-active,.el-pagination.is-background .el-pager li.is-disabled.is-active,.el-pagination.is-background .el-pager li:disabled.is-active{background-color:var(--el-fill-color-dark);color:var(--el-text-color-secondary)}.el-pagination.is-background .btn-prev{margin-left:var(--el-pagination-item-gap)}.el-pagination--small .btn-next,.el-pagination--small .btn-prev,.el-pagination--small .el-pager li{font-size:var(--el-pagination-font-size-small);height:var(--el-pagination-button-height-small);line-height:var(--el-pagination-button-height-small);min-width:var(--el-pagination-button-width-small)}.el-pagination--small button,.el-pagination--small span:not([class*=suffix]){font-size:var(--el-pagination-font-size-small)}.el-pagination--small .el-select{width:100px}.el-pagination--large .btn-next,.el-pagination--large .btn-prev,.el-pagination--large .el-pager li{height:var(--el-pagination-button-height-large);line-height:var(--el-pagination-button-height-large);min-width:var(--el-pagination-button-width-large)}.el-pagination--large .el-select .el-input{width:160px}.el-pager{font-size:0;list-style:none;margin:0;padding:0;-webkit-user-select:none;-moz-user-select:none;user-select:none}.el-pager,.el-pager li{align-items:center;display:flex}.el-pager li{background:var(--el-pagination-bg-color);border:none;border-radius:var(--el-pagination-border-radius);box-sizing:border-box;color:var(--el-pagination-button-color);cursor:pointer;font-size:var(--el-pagination-font-size);height:var(--el-pagination-button-height);justify-content:center;line-height:var(--el-pagination-button-height);min-width:var(--el-pagination-button-width);padding:0 4px;text-align:center}.el-pager li *{pointer-events:none}.el-pager li:focus{outline:none}.el-pager li.is-active,.el-pager li:hover{color:var(--el-pagination-hover-color)}.el-pager li.is-active{cursor:default;font-weight:700}.el-pager li.is-active.is-disabled{color:var(--el-text-color-secondary);font-weight:700}.el-pager li.is-disabled,.el-pager li:disabled{background-color:var(--el-pagination-button-disabled-bg-color);color:var(--el-pagination-button-disabled-color);cursor:not-allowed}.el-pager li:focus-visible{outline:1px solid var(--el-pagination-hover-color);outline-offset:-1px}.el-popconfirm__main{align-items:center;display:flex}.el-popconfirm__icon{margin-right:5px}.el-popconfirm__action{margin-top:8px;text-align:right}.el-popover{--el-popover-bg-color:var(--el-bg-color-overlay);--el-popover-font-size:var(--el-font-size-base);--el-popover-border-color:var(--el-border-color-lighter);--el-popover-padding:12px;--el-popover-padding-large:18px 20px;--el-popover-title-font-size:16px;--el-popover-title-text-color:var(--el-text-color-primary);--el-popover-border-radius:4px}.el-popover.el-popper{background:var(--el-popover-bg-color);border:1px solid var(--el-popover-border-color);border-radius:var(--el-popover-border-radius);box-shadow:var(--el-box-shadow-light);box-sizing:border-box;color:var(--el-text-color-regular);font-size:var(--el-popover-font-size);line-height:1.4;min-width:150px;overflow-wrap:break-word;padding:var(--el-popover-padding);z-index:var(--el-index-popper)}.el-popover.el-popper--plain{padding:var(--el-popover-padding-large)}.el-popover__title{color:var(--el-popover-title-text-color);font-size:var(--el-popover-title-font-size);line-height:1;margin-bottom:12px}.el-popover__reference:focus:hover,.el-popover__reference:focus:not(.focusing){outline-width:0}.el-popover.el-popper.is-dark{--el-popover-bg-color:var(--el-text-color-primary);--el-popover-border-color:var(--el-text-color-primary);--el-popover-title-text-color:var(--el-bg-color);color:var(--el-bg-color)}.el-popover.el-popper:focus,.el-popover.el-popper:focus:active{outline-width:0}.el-progress{align-items:center;display:flex;line-height:1;position:relative}.el-progress__text{color:var(--el-text-color-regular);font-size:14px;line-height:1;margin-left:5px;min-width:50px}.el-progress__text i{display:block;vertical-align:middle}.el-progress--circle,.el-progress--dashboard{display:inline-block}.el-progress--circle .el-progress__text,.el-progress--dashboard .el-progress__text{left:0;margin:0;position:absolute;text-align:center;top:50%;transform:translateY(-50%);width:100%}.el-progress--circle .el-progress__text i,.el-progress--dashboard .el-progress__text i{display:inline-block;vertical-align:middle}.el-progress--without-text .el-progress__text{display:none}.el-progress--without-text .el-progress-bar{display:block;margin-right:0;padding-right:0}.el-progress--text-inside .el-progress-bar{margin-right:0;padding-right:0}.el-progress.is-success .el-progress-bar__inner{background-color:var(--el-color-success)}.el-progress.is-success .el-progress__text{color:var(--el-color-success)}.el-progress.is-warning .el-progress-bar__inner{background-color:var(--el-color-warning)}.el-progress.is-warning .el-progress__text{color:var(--el-color-warning)}.el-progress.is-exception .el-progress-bar__inner{background-color:var(--el-color-danger)}.el-progress.is-exception .el-progress__text{color:var(--el-color-danger)}.el-progress-bar{box-sizing:border-box;flex-grow:1}.el-progress-bar__outer{background-color:var(--el-border-color-lighter);border-radius:100px;height:6px;overflow:hidden;position:relative;vertical-align:middle}.el-progress-bar__inner{background-color:var(--el-color-primary);border-radius:100px;height:100%;left:0;line-height:1;position:absolute;text-align:right;top:0;transition:width .6s ease;white-space:nowrap}.el-progress-bar__inner:after{content:"";display:inline-block;height:100%;vertical-align:middle}.el-progress-bar__inner--indeterminate{animation:indeterminate 3s infinite;transform:translateZ(0)}.el-progress-bar__inner--striped{background-image:linear-gradient(45deg,rgba(0,0,0,.1) 25%,transparent 0,transparent 50%,rgba(0,0,0,.1) 0,rgba(0,0,0,.1) 75%,transparent 0,transparent);background-size:1.25em 1.25em}.el-progress-bar__inner--striped.el-progress-bar__inner--striped-flow{animation:striped-flow 3s linear infinite}.el-progress-bar__innerText{color:#fff;display:inline-block;font-size:12px;margin:0 5px;vertical-align:middle}@keyframes progress{0%{background-position:0 0}to{background-position:32px 0}}@keyframes indeterminate{0%{left:-100%}to{left:100%}}@keyframes striped-flow{0%{background-position:-100%}to{background-position:100%}}.el-radio-button{--el-radio-button-checked-bg-color:var(--el-color-primary);--el-radio-button-checked-text-color:var(--el-color-white);--el-radio-button-checked-border-color:var(--el-color-primary);--el-radio-button-disabled-checked-fill:var(--el-border-color-extra-light)}.el-radio-button,.el-radio-button__inner{display:inline-block;outline:none;position:relative}.el-radio-button__inner{-webkit-appearance:none;background:var(--el-button-bg-color,var(--el-fill-color-blank));border:var(--el-border);border-left:0;border-radius:0;box-sizing:border-box;color:var(--el-button-text-color,var(--el-text-color-regular));cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-button-font-weight,var(--el-font-weight-primary));line-height:1;margin:0;padding:8px 15px;text-align:center;transition:var(--el-transition-all);-webkit-user-select:none;-moz-user-select:none;user-select:none;vertical-align:middle;white-space:nowrap}.el-radio-button__inner.is-round{padding:8px 15px}.el-radio-button__inner:hover{color:var(--el-color-primary)}.el-radio-button__inner [class*=el-icon-]{line-height:.9}.el-radio-button__inner [class*=el-icon-]+span{margin-left:5px}.el-radio-button:first-child .el-radio-button__inner{border-left:var(--el-border);border-radius:var(--el-border-radius-base) 0 0 var(--el-border-radius-base);box-shadow:none!important}.el-radio-button.is-active .el-radio-button__original-radio:not(:disabled)+.el-radio-button__inner{background-color:var(--el-radio-button-checked-bg-color,var(--el-color-primary));border-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));box-shadow:-1px 0 0 0 var(--el-radio-button-checked-border-color,var(--el-color-primary));color:var(--el-radio-button-checked-text-color,var(--el-color-white))}.el-radio-button__original-radio{opacity:0;outline:none;position:absolute;z-index:-1}.el-radio-button__original-radio:focus-visible+.el-radio-button__inner{border-left:var(--el-border);border-left-color:var(--el-radio-button-checked-border-color,var(--el-color-primary));border-radius:var(--el-border-radius-base);box-shadow:none;outline:2px solid var(--el-radio-button-checked-border-color);outline-offset:1px;z-index:2}.el-radio-button__original-radio:disabled+.el-radio-button__inner{background-color:var(--el-button-disabled-bg-color,var(--el-fill-color-blank));background-image:none;border-color:var(--el-button-disabled-border-color,var(--el-border-color-light));box-shadow:none;color:var(--el-disabled-text-color);cursor:not-allowed}.el-radio-button__original-radio:disabled:checked+.el-radio-button__inner{background-color:var(--el-radio-button-disabled-checked-fill)}.el-radio-button:last-child .el-radio-button__inner{border-radius:0 var(--el-border-radius-base) var(--el-border-radius-base) 0}.el-radio-button:first-child:last-child .el-radio-button__inner{border-radius:var(--el-border-radius-base)}.el-radio-button--large .el-radio-button__inner{border-radius:0;font-size:var(--el-font-size-base);padding:12px 19px}.el-radio-button--large .el-radio-button__inner.is-round{padding:12px 19px}.el-radio-button--small .el-radio-button__inner{border-radius:0;font-size:12px;padding:5px 11px}.el-radio-button--small .el-radio-button__inner.is-round{padding:5px 11px}.el-radio-group{flex-wrap:wrap;font-size:0}.el-radio,.el-radio-group{align-items:center;display:inline-flex}.el-radio{--el-radio-font-size:var(--el-font-size-base);--el-radio-text-color:var(--el-text-color-regular);--el-radio-font-weight:var(--el-font-weight-primary);--el-radio-input-height:14px;--el-radio-input-width:14px;--el-radio-input-border-radius:var(--el-border-radius-circle);--el-radio-input-bg-color:var(--el-fill-color-blank);--el-radio-input-border:var(--el-border);--el-radio-input-border-color:var(--el-border-color);--el-radio-input-border-color-hover:var(--el-color-primary);color:var(--el-radio-text-color);cursor:pointer;font-size:var(--el-font-size-base);font-weight:var(--el-radio-font-weight);height:32px;margin-right:30px;outline:none;position:relative;-webkit-user-select:none;-moz-user-select:none;user-select:none;white-space:nowrap}.el-radio.el-radio--large{height:40px}.el-radio.el-radio--small{height:24px}.el-radio.is-bordered{border:var(--el-border);border-radius:var(--el-border-radius-base);box-sizing:border-box;padding:0 15px 0 9px}.el-radio.is-bordered.is-checked{border-color:var(--el-color-primary)}.el-radio.is-bordered.is-disabled{border-color:var(--el-border-color-lighter);cursor:not-allowed}.el-radio.is-bordered.el-radio--large{border-radius:var(--el-border-radius-base);padding:0 19px 0 11px}.el-radio.is-bordered.el-radio--large .el-radio__label{font-size:var(--el-font-size-base)}.el-radio.is-bordered.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.is-bordered.el-radio--small{border-radius:var(--el-border-radius-base);padding:0 11px 0 7px}.el-radio.is-bordered.el-radio--small .el-radio__label{font-size:12px}.el-radio.is-bordered.el-radio--small .el-radio__inner{height:12px;width:12px}.el-radio:last-child{margin-right:0}.el-radio__input{cursor:pointer;display:inline-flex;outline:none;position:relative;vertical-align:middle;white-space:nowrap}.el-radio__input.is-disabled .el-radio__inner{border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled .el-radio__inner,.el-radio__input.is-disabled .el-radio__inner:after{background-color:var(--el-disabled-bg-color);cursor:not-allowed}.el-radio__input.is-disabled .el-radio__inner+.el-radio__label{cursor:not-allowed}.el-radio__input.is-disabled.is-checked .el-radio__inner{background-color:var(--el-disabled-bg-color);border-color:var(--el-disabled-border-color)}.el-radio__input.is-disabled.is-checked .el-radio__inner:after{background-color:var(--el-text-color-placeholder)}.el-radio__input.is-disabled+span.el-radio__label{color:var(--el-text-color-placeholder);cursor:not-allowed}.el-radio__input.is-checked .el-radio__inner{background:var(--el-color-primary);border-color:var(--el-color-primary)}.el-radio__input.is-checked .el-radio__inner:after{transform:translate(-50%,-50%) scale(1)}.el-radio__input.is-checked+.el-radio__label{color:var(--el-color-primary)}.el-radio__input.is-focus .el-radio__inner{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner{background-color:var(--el-radio-input-bg-color);border:var(--el-radio-input-border);border-radius:var(--el-radio-input-border-radius);box-sizing:border-box;cursor:pointer;display:inline-block;height:var(--el-radio-input-height);position:relative;width:var(--el-radio-input-width)}.el-radio__inner:hover{border-color:var(--el-radio-input-border-color-hover)}.el-radio__inner:after{background-color:var(--el-color-white);border-radius:var(--el-radio-input-border-radius);content:"";height:4px;left:50%;position:absolute;top:50%;transform:translate(-50%,-50%) scale(0);transition:transform .15s ease-in;width:4px}.el-radio__original{bottom:0;left:0;margin:0;opacity:0;outline:none;position:absolute;right:0;top:0;z-index:-1}.el-radio__original:focus-visible+.el-radio__inner{border-radius:var(--el-radio-input-border-radius);outline:2px solid var(--el-radio-input-border-color-hover);outline-offset:1px}.el-radio:focus:not(:focus-visible):not(.is-focus):not(:active):not(.is-disabled) .el-radio__inner{box-shadow:0 0 2px 2px var(--el-radio-input-border-color-hover)}.el-radio__label{font-size:var(--el-radio-font-size);padding-left:8px}.el-radio.el-radio--large .el-radio__label{font-size:14px}.el-radio.el-radio--large .el-radio__inner{height:14px;width:14px}.el-radio.el-radio--small .el-radio__label{font-size:12px}.el-radio.el-radio--small 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.el-transfer-panel__header{align-items:center;background:var(--el-transfer-panel-header-bg-color);border:1px solid var(--el-transfer-border-color);border-top-left-radius:var(--el-transfer-border-radius);border-top-right-radius:var(--el-transfer-border-radius);box-sizing:border-box;color:var(--el-color-black);display:flex;height:var(--el-transfer-panel-header-height);margin:0;padding-left:15px}.el-transfer-panel .el-transfer-panel__header .el-checkbox{align-items:center;display:flex;position:relative;width:100%}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label{color:var(--el-text-color-primary);font-size:16px;font-weight:400}.el-transfer-panel .el-transfer-panel__header .el-checkbox .el-checkbox__label span{color:var(--el-text-color-secondary);font-size:12px;font-weight:400;position:absolute;right:15px;top:50%;transform:translate3d(0,-50%,0)}.el-transfer-panel .el-transfer-panel__footer{background:var(--el-bg-color-overlay);border:1px solid 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0;overflow:hidden;padding:0;width:var(--el-upload-list-picture-card-size)}.el-upload-list--picture-card .el-upload-list__item .el-icon--check,.el-upload-list--picture-card .el-upload-list__item .el-icon--circle-check{color:#fff}.el-upload-list--picture-card .el-upload-list__item .el-icon--close{display:none}.el-upload-list--picture-card .el-upload-list__item:hover .el-upload-list__item-status-label{display:block;opacity:0}.el-upload-list--picture-card .el-upload-list__item:hover .el-progress__text{display:block}.el-upload-list--picture-card .el-upload-list__item .el-upload-list__item-name{display:none}.el-upload-list--picture-card .el-upload-list__item-thumbnail{height:100%;-o-object-fit:contain;object-fit:contain;width:100%}.el-upload-list--picture-card .el-upload-list__item-status-label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-list--picture-card .el-upload-list__item-status-label 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img{display:block;height:100%;width:100%}.el-upload-cover__label{background:var(--el-color-success);height:24px;right:-15px;text-align:center;top:-6px;transform:rotate(45deg);width:40px}.el-upload-cover__label i{color:#fff;font-size:12px;margin-top:11px;transform:rotate(-45deg)}.el-upload-cover__progress{display:inline-block;position:static;vertical-align:middle;width:243px}.el-upload-cover__progress+.el-upload__inner{opacity:0}.el-upload-cover__content{height:100%;left:0;position:absolute;top:0;width:100%}.el-upload-cover__interact{background-color:var(--el-overlay-color-light);bottom:0;height:100%;left:0;position:absolute;text-align:center;width:100%}.el-upload-cover__interact .btn{color:#fff;cursor:pointer;display:inline-block;font-size:14px;margin-top:60px;transition:var(--el-transition-md-fade);vertical-align:middle}.el-upload-cover__interact .btn i{margin-top:0}.el-upload-cover__interact .btn span{opacity:0;transition:opacity .15s linear}.el-upload-cover__interact 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var(--el-border-color-light)}.el-popper.is-light>.el-popper__arrow:before{right:0}.el-popper.is-pure{padding:0}.el-popper__arrow,.el-popper__arrow:before{height:10px;position:absolute;width:10px;z-index:-1}.el-popper__arrow:before{background:var(--el-text-color-primary);box-sizing:border-box;content:" 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data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 2x" class="cursor-pointer py-2" src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div class="grow"></div><li class="el-menu-item" role="menuitem" tabindex="-1"><!--[--><!--[-->HOME<!--]--><!--[--><!--]--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Project<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Wet Lab<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Dry Lab<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Team<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li><li class="el-sub-menu" role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Human Practices<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li></ul><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-mobile px-10 flex lg:hidden"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 2x" class="cursor-pointer py-2" src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div class="grow"></div><!--[--><span class="el-avatar el-avatar--icon el-avatar--circle self-center cursor-pointer el-tooltip__trigger el-tooltip__trigger" style=""><i class="el-icon" style=""><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M160 448a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zM160 896a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32z"></path></svg><!--]--></i></span><!--teleport start--><!--teleport end--><!--]--></ul></header><div class="pt-[8rem]"><!--[--><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="cover" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/project.webp 1x, https://static.igem.wiki/teams/5358/banner/project.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/project.webp"><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg">Safety</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[300px] md:mr-10"><h1 class="mt-0">TOC</h1><ul><!--[--><li class="my-5"><a class="cursor-pointer">The rules and policies of the iGEM</a></li><li class="my-5"><a class="cursor-pointer">Project safety</a></li><li class="my-5"><a class="cursor-pointer">Laboratory security</a></li><!--]--></ul></aside><div class="content"><p><!--[-->Synthetic biology has the ability to change the world in amazing ways. From treating cancer to alleviating the climate crisis, we do have the ability to build a better world at iGEM. However, by working with some unpredictable organisms, we must also understand that the good works we create can actually cause harm. When looking at the big picture, we must also understand that we must be carefully every day. As researchers, we need to manage the community and environmental risks.<!--]--></p><h2 id="the-rules-and-policies-of-the-igem"><!--[-->The rules and policies of the iGEM<!--]--></h2><ol><!--[--><li><!--[-->Release Beyond Containment: You cannot release or deploy any genetically modified organisms outside the lab. You must Check In before bringing any product of synthetic biology outside of the lab.<!--]--></li><li><!--[-->White List: You must Check In before beginning experiments with any organism, part, or activity that is not on the White List.<!--]--></li><li><!--[-->No Human Experimentation: Testing your project on humans (including yourselves) is strictly prohibited. You must check in before beginning any laboratory experiments involving humans or human samples (including but not limited to blood, DNA, other bodily specimens, and health or psychological outcomes).<!--]--></li><li><!--[-->Animal Use: You must Check In and submit an Animal Use Form before beginning any experiments involving animals or animal samples. This includes vertebrates (e.g. rats, mice, guinea pigs, hamsters, amphibians, and fishes) and higher order invertebrates(e.g. cuttlefish, octopus, squid, lobster, bees, or American or German cockroaches).<!--]--></li><li><!--[-->Human Subjects Research: All human subjects research (e.g.surveys, interviews, public engagement) you carry out must be done in accordance with relevant laws and regulations, as well as any institutional rules or guidance.<!--]--></li><li><!--[-->Antimicrobial Resistance: You must Check In before beginning experiments likely to increase the risk posed by antimicrobial resistance (e.g. by using novel resistance factors, adding known factors into new organisms, or resistance factors not previously used in your facility).<!--]--></li><li><!--[-->Gene Drive: Gene Drive is not allowed in iGEM projects without a special exception from the Safety &amp; Security Committee.<!--]--></li><li><!--[-->Coronavirus: Any work using live SARS-CoV-2 virus(the causative agent of COVID-19) must follow World Health Organization (WHO) guidance, in addition to all local and institutional rules. WHO guidance may be stricter than local safety rules. You must Check In before beginning experiments with live SARS-CoV-2 or parts from SARS-CoV-2.<!--]--></li><li><!--[-->Environmental Samples: As long as parts or organisms are not isolated from them, samples from the environment, the food industry, or other non- traditional suppliers may be used in your experiments You must Check In before using any parts organisms obtained from outside the lab or from non- traditional suppliers.<!--]--></li><!--]--></ol><h2 id="project-safety"><!--[-->Project safety<!--]--></h2><h3 id="safety-of-project-design"><!--[-->Safety of project design<!--]--></h3><p><!--[-->Our project employs the insertion of RecA intein into T7 RNAP to detect endocrine disruption effects and utilizes the SOS response mechanism of Escherichia coli to detect DNA damage. Additionally, the LuxI/R system is introduced to achieve an AND gate detection of both effects. Furthermore, the Hrp gene regulation system and BsFbFp fluorescent gene are combined to amplify the transcriptional signal and ensure effective signal expression under anaerobic conditions; in the hardware section, we have designed a high-throughput platform for automated sample detection. Throughout our entire experimental process, our team has conducted experiments using qualified E. coli strains such as BL21, DH5a, and MG1655, as well as plasmid vectors pUC19 and pET28. These E. coli strains are classified as Biosafety Level I and have been handled in a sterile environment.<!--]--></p><p><!--[-->According to the WHO Laboratory Biosafety Manual, the risks involved in our experiments are Level I (no risk or very low risk).<!--]--></p><h3 id="project-hardware-security"><!--[-->Project Hardware Security<!--]--></h3><ul><!--[--><li><!--[-->Disposable Reaction Vessels: We utilize disposable PCR tubes as reaction vessels, which means that there is no need for contact with the biological system at the start of the reaction, thus eliminating concerns about genetic modification organism contamination.<!--]--></li><li><!--[-->No Direct Contact: Users only need to add the sample liquid to the reaction chamber; unless necessary, it is not recommended to open our device.<!--]--></li><li><!--[-->Sealed Containers: The reaction chamber is completely sealed, so unless the user intentionally breaks it, there will be no opportunity for the user to come into contact with the reagents inside.<!--]--></li><li><!--[-->Automated Control: All operations after adding the sample can be controlled automatically through a mobile app, without the need for manual operation of the device.<!--]--></li><li><!--[-->Modular Design: If the device is damaged, only the specific part needs to be replaced, without the need for disassembly.<!--]--></li><!--]--></ul><h3 id="engineering-bacterial-system"><!--[-->Engineering Bacterial System<!--]--></h3><p><!--[-->Our project utilizes Escherichia coli as an engineered strain, relying on genetically designed circuits to specifically identify trace amounts of endocrine disruptors and elicit a response. In this process, the engineered strain&#39;s response is susceptible to factors such as cellular status, physiological conditions, and environmental influences; moreover, there is a possibility of the substances produced by the reaction leaking into the environment.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="safety-hardware" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/safety/hardware.webp 1x, https://static.igem.wiki/teams/5358/safety/hardware.webp 2x" src="https://static.igem.wiki/teams/5358/safety/hardware.webp"><!--]--></p><p><!--[-->Our hardware is capable of addressing these challenges. The device is designed using a modular approach. We provide users with sealed reaction vessels, thereby minimizing the need for direct contact with chemical reagents. The device features a light-blocking cover and a constant temperature control system that provide our Escherichia coli with a stable, light-protected, and temperature-controlled environment to match their optimal working conditions.<!--]--></p><p><!--[-->To prevent contamination of our system and the environment, we have implemented the following designs:<!--]--></p><ol><!--[--><li><!--[-->The fluorescence detection module is covered with a black nylon protective sleeve to provide a dark chamber, reducing the interference of background light on the detection results.<!--]--></li><li><!--[-->The cover is extended around to prevent substances produced by the reaction from escaping into the atmosphere, ensuring biosafety.<!--]--></li><li><!--[-->Disposable reaction vessels are used, and they are subjected to specialized biosafety treatment after the detection is completed.<!--]--></li><!--]--></ol><h2 id="laboratory-security"><!--[-->Laboratory security<!--]--></h2><p><!--[-->All laboratories in China must be accredited by the Office of the Ministry of Environment in accordance with the standard <code class=""><!--[-->LST EN ISO/IEC 17025:2018<!--]--></code>. To ensure safety, team members must comply with all essential safety protocols before working in different laboratories. Prior to entering the lab, each team member is required to familiarize themselves with the experimental manual beforehand, and to receive training on experimental safety measures.<!--]--></p><p><!--[-->All team members must familiar themselves with the safety features in the lab, such as<!--]--></p><ol><!--[--><li><!--[-->Fire Extinguisher;<!--]--></li><li><!--[-->First Aid Kit;<!--]--></li><li><!--[-->Biosafety Cabinet;<!--]--></li><li><!--[-->Flame Retardant Benches;<!--]--></li><li><!--[-->the Nearest Safety Shower;<!--]--></li><!--]--></ol><p><!--[-->Wastes after experiments must be properly disposed, following not only following rules,<!--]--></p><ol><!--[--><li><!--[-->Used pipette tips, gels, eppendorf and so on, should be threw into particular biohazard waste bins;<!--]--></li><li><!--[-->Bacteria solutions and reagents could be disposed only after high-pressure sterilization;<!--]--></li><li><!--[-->Toxic or acidic/basic reagents must be neutralized before disposal;<!--]--></li><!--]--></ol><p><!--[-->Other rules such as,<!--]--></p><ol><!--[--><li><!--[-->Pay attention to the disinfection of the test bench and experimental equipment, and the equipment contacting biological materials should be autoclaved;<!--]--></li><li><!--[-->Properly handle and store hazardous and(or) combustible materials;<!--]--></li><li><!--[-->Dress code: lab coats, masks, trousers(shorts are forbidden);<!--]--></li><li><!--[-->Long hair should be tied up;<!--]--></li><li><!--[-->etc.<!--]--></li><!--]--></ol><p><!--[-->Our experiment team strictly abides by the following experiment manuals or regulations throughout the experiment:<!--]--></p><p><!--[-->WHO,Laboratory Biosafety Manual<!--]--></p><p><!--[--><a href="https://www.who.int/zh/publications/i/item/9789240011335#" rel="nofollow"><!--[-->https://www.who.int/zh/publications/i/item/9789240011335#<!--]--></a><!--]--></p><p><!--[-->Classified Catalogue of Medical Wastes in China<!--]--></p><p><!--[--><a href="https://www.mee.gov.cn/ywgz/gtfwyhxpgl/wxfw/200504/t20050418_65998.shtml" rel="nofollow"><!--[-->https://www.mee.gov.cn/ywgz/gtfwyhxpgl/wxfw/200504/t20050418_65998.shtml<!--]--></a><!--]--></p><p><!--[-->Standards for special packaging bags, containers, and warning symbols for medical waste<!--]--></p><p><!--[--><a href="http://www.mee.gov.cn/gkml/zj/wj/200910/t20091022_172239.htm" rel="nofollow"><!--[-->http://www.mee.gov.cn/gkml/zj/wj/200910/t20091022_172239.htm<!--]--></a><!--]--></p></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a aria-current="page" href="/bit/safety" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a 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role="menuitem" ariahaspopup="true" ariaexpanded="false"><!--[--><div class="el-sub-menu__title el-tooltip__trigger el-tooltip__trigger"><!--[-->Human Practices<!--]--><i class="el-icon el-sub-menu__icon-arrow" style="transform:none;"><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M831.872 340.864 512 652.672 192.128 340.864a30.592 30.592 0 0 0-42.752 0 29.12 29.12 0 0 0 0 41.6L489.664 714.24a32 32 0 0 0 44.672 0l340.288-331.712a29.12 29.12 0 0 0 0-41.728 30.592 30.592 0 0 0-42.752 0z"></path></svg><!--]--></i></div><!--teleport start--><!--teleport end--><!--]--></li></ul><ul role="menubar" style="--el-menu-level:0;" class="el-menu el-menu--horizontal menu-mobile px-10 flex lg:hidden"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 1x, https://static.igem.wiki/teams/5358/icons/logo.png?quality=100 2x" class="cursor-pointer py-2" src="https://static.igem.wiki/teams/5358/icons/logo.png?quality=100"><div class="grow"></div><!--[--><span class="el-avatar el-avatar--icon el-avatar--circle self-center cursor-pointer el-tooltip__trigger el-tooltip__trigger" style=""><i class="el-icon" style=""><!--[--><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 1024 1024"><path fill="currentColor" d="M160 448a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V160.064a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32V416a32 32 0 0 1-32 32zM160 896a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h256a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32zm448 0a32 32 0 0 1-32-32V608a32 32 0 0 1 32-32h255.936a32 32 0 0 1 32 32v256a32 32 0 0 1-32 32z"></path></svg><!--]--></i></span><!--teleport start--><!--teleport end--><!--]--></ul></header><div class="pt-[8rem]"><!--[--><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="cover" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/banner/project.webp 1x, https://static.igem.wiki/teams/5358/banner/project.webp 2x" class="inline-block w-full mt-[-1rem]" src="https://static.igem.wiki/teams/5358/banner/project.webp"><div class="page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg">Safety</div><div class="flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"><!--[--><aside class="md:sticky md:top-[120px] md:w-[200px] md:h-[300px] md:mr-10"><h1 class="mt-0">TOC</h1><ul><!--[--><li class="my-5"><a class="cursor-pointer">The rules and policies of the iGEM</a></li><li class="my-5"><a class="cursor-pointer">Project safety</a></li><li class="my-5"><a class="cursor-pointer">Laboratory security</a></li><!--]--></ul></aside><div class="content"><p><!--[-->Synthetic biology has the ability to change the world in amazing ways. From treating cancer to alleviating the climate crisis, we do have the ability to build a better world at iGEM. However, by working with some unpredictable organisms, we must also understand that the good works we create can actually cause harm. When looking at the big picture, we must also understand that we must be carefully every day. As researchers, we need to manage the community and environmental risks.<!--]--></p><h2 id="the-rules-and-policies-of-the-igem"><!--[-->The rules and policies of the iGEM<!--]--></h2><ol><!--[--><li><!--[-->Release Beyond Containment: You cannot release or deploy any genetically modified organisms outside the lab. You must Check In before bringing any product of synthetic biology outside of the lab.<!--]--></li><li><!--[-->White List: You must Check In before beginning experiments with any organism, part, or activity that is not on the White List.<!--]--></li><li><!--[-->No Human Experimentation: Testing your project on humans (including yourselves) is strictly prohibited. You must check in before beginning any laboratory experiments involving humans or human samples (including but not limited to blood, DNA, other bodily specimens, and health or psychological outcomes).<!--]--></li><li><!--[-->Animal Use: You must Check In and submit an Animal Use Form before beginning any experiments involving animals or animal samples. This includes vertebrates (e.g. rats, mice, guinea pigs, hamsters, amphibians, and fishes) and higher order invertebrates(e.g. cuttlefish, octopus, squid, lobster, bees, or American or German cockroaches).<!--]--></li><li><!--[-->Human Subjects Research: All human subjects research (e.g.surveys, interviews, public engagement) you carry out must be done in accordance with relevant laws and regulations, as well as any institutional rules or guidance.<!--]--></li><li><!--[-->Antimicrobial Resistance: You must Check In before beginning experiments likely to increase the risk posed by antimicrobial resistance (e.g. by using novel resistance factors, adding known factors into new organisms, or resistance factors not previously used in your facility).<!--]--></li><li><!--[-->Gene Drive: Gene Drive is not allowed in iGEM projects without a special exception from the Safety &amp; Security Committee.<!--]--></li><li><!--[-->Coronavirus: Any work using live SARS-CoV-2 virus(the causative agent of COVID-19) must follow World Health Organization (WHO) guidance, in addition to all local and institutional rules. WHO guidance may be stricter than local safety rules. You must Check In before beginning experiments with live SARS-CoV-2 or parts from SARS-CoV-2.<!--]--></li><li><!--[-->Environmental Samples: As long as parts or organisms are not isolated from them, samples from the environment, the food industry, or other non- traditional suppliers may be used in your experiments You must Check In before using any parts organisms obtained from outside the lab or from non- traditional suppliers.<!--]--></li><!--]--></ol><h2 id="project-safety"><!--[-->Project safety<!--]--></h2><h3 id="safety-of-project-design"><!--[-->Safety of project design<!--]--></h3><p><!--[-->Our project employs the insertion of RecA intein into T7 RNAP to detect endocrine disruption effects and utilizes the SOS response mechanism of Escherichia coli to detect DNA damage. Additionally, the LuxI/R system is introduced to achieve an AND gate detection of both effects. Furthermore, the Hrp gene regulation system and BsFbFp fluorescent gene are combined to amplify the transcriptional signal and ensure effective signal expression under anaerobic conditions; in the hardware section, we have designed a high-throughput platform for automated sample detection. Throughout our entire experimental process, our team has conducted experiments using qualified E. coli strains such as BL21, DH5a, and MG1655, as well as plasmid vectors pUC19 and pET28. These E. coli strains are classified as Biosafety Level I and have been handled in a sterile environment.<!--]--></p><p><!--[-->According to the WHO Laboratory Biosafety Manual, the risks involved in our experiments are Level I (no risk or very low risk).<!--]--></p><h3 id="project-hardware-security"><!--[-->Project Hardware Security<!--]--></h3><ul><!--[--><li><!--[-->Disposable Reaction Vessels: We utilize disposable PCR tubes as reaction vessels, which means that there is no need for contact with the biological system at the start of the reaction, thus eliminating concerns about genetic modification organism contamination.<!--]--></li><li><!--[-->No Direct Contact: Users only need to add the sample liquid to the reaction chamber; unless necessary, it is not recommended to open our device.<!--]--></li><li><!--[-->Sealed Containers: The reaction chamber is completely sealed, so unless the user intentionally breaks it, there will be no opportunity for the user to come into contact with the reagents inside.<!--]--></li><li><!--[-->Automated Control: All operations after adding the sample can be controlled automatically through a mobile app, without the need for manual operation of the device.<!--]--></li><li><!--[-->Modular Design: If the device is damaged, only the specific part needs to be replaced, without the need for disassembly.<!--]--></li><!--]--></ul><h3 id="engineering-bacterial-system"><!--[-->Engineering Bacterial System<!--]--></h3><p><!--[-->Our project utilizes Escherichia coli as an engineered strain, relying on genetically designed circuits to specifically identify trace amounts of endocrine disruptors and elicit a response. In this process, the engineered strain&#39;s response is susceptible to factors such as cellular status, physiological conditions, and environmental influences; moreover, there is a possibility of the substances produced by the reaction leaking into the environment.<!--]--></p><p><!--[--><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" alt="safety-hardware" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/safety/hardware.webp 1x, https://static.igem.wiki/teams/5358/safety/hardware.webp 2x" src="https://static.igem.wiki/teams/5358/safety/hardware.webp"><!--]--></p><p><!--[-->Our hardware is capable of addressing these challenges. The device is designed using a modular approach. We provide users with sealed reaction vessels, thereby minimizing the need for direct contact with chemical reagents. The device features a light-blocking cover and a constant temperature control system that provide our Escherichia coli with a stable, light-protected, and temperature-controlled environment to match their optimal working conditions.<!--]--></p><p><!--[-->To prevent contamination of our system and the environment, we have implemented the following designs:<!--]--></p><ol><!--[--><li><!--[-->The fluorescence detection module is covered with a black nylon protective sleeve to provide a dark chamber, reducing the interference of background light on the detection results.<!--]--></li><li><!--[-->The cover is extended around to prevent substances produced by the reaction from escaping into the atmosphere, ensuring biosafety.<!--]--></li><li><!--[-->Disposable reaction vessels are used, and they are subjected to specialized biosafety treatment after the detection is completed.<!--]--></li><!--]--></ol><h2 id="laboratory-security"><!--[-->Laboratory security<!--]--></h2><p><!--[-->All laboratories in China must be accredited by the Office of the Ministry of Environment in accordance with the standard <code class=""><!--[-->LST EN ISO/IEC 17025:2018<!--]--></code>. To ensure safety, team members must comply with all essential safety protocols before working in different laboratories. Prior to entering the lab, each team member is required to familiarize themselves with the experimental manual beforehand, and to receive training on experimental safety measures.<!--]--></p><p><!--[-->All team members must familiar themselves with the safety features in the lab, such as<!--]--></p><ol><!--[--><li><!--[-->Fire Extinguisher;<!--]--></li><li><!--[-->First Aid Kit;<!--]--></li><li><!--[-->Biosafety Cabinet;<!--]--></li><li><!--[-->Flame Retardant Benches;<!--]--></li><li><!--[-->the Nearest Safety Shower;<!--]--></li><!--]--></ol><p><!--[-->Wastes after experiments must be properly disposed, following not only following rules,<!--]--></p><ol><!--[--><li><!--[-->Used pipette tips, gels, eppendorf and so on, should be threw into particular biohazard waste bins;<!--]--></li><li><!--[-->Bacteria solutions and reagents could be disposed only after high-pressure sterilization;<!--]--></li><li><!--[-->Toxic or acidic/basic reagents must be neutralized before disposal;<!--]--></li><!--]--></ol><p><!--[-->Other rules such as,<!--]--></p><ol><!--[--><li><!--[-->Pay attention to the disinfection of the test bench and experimental equipment, and the equipment contacting biological materials should be autoclaved;<!--]--></li><li><!--[-->Properly handle and store hazardous and(or) combustible materials;<!--]--></li><li><!--[-->Dress code: lab coats, masks, trousers(shorts are forbidden);<!--]--></li><li><!--[-->Long hair should be tied up;<!--]--></li><li><!--[-->etc.<!--]--></li><!--]--></ol><p><!--[-->Our experiment team strictly abides by the following experiment manuals or regulations throughout the experiment:<!--]--></p><p><!--[-->WHO,Laboratory Biosafety Manual<!--]--></p><p><!--[--><a href="https://www.who.int/zh/publications/i/item/9789240011335#" rel="nofollow"><!--[-->https://www.who.int/zh/publications/i/item/9789240011335#<!--]--></a><!--]--></p><p><!--[-->Classified Catalogue of Medical Wastes in China<!--]--></p><p><!--[--><a href="https://www.mee.gov.cn/ywgz/gtfwyhxpgl/wxfw/200504/t20050418_65998.shtml" rel="nofollow"><!--[-->https://www.mee.gov.cn/ywgz/gtfwyhxpgl/wxfw/200504/t20050418_65998.shtml<!--]--></a><!--]--></p><p><!--[-->Standards for special packaging bags, containers, and warning symbols for medical waste<!--]--></p><p><!--[--><a href="http://www.mee.gov.cn/gkml/zj/wj/200910/t20091022_172239.htm" rel="nofollow"><!--[-->http://www.mee.gov.cn/gkml/zj/wj/200910/t20091022_172239.htm<!--]--></a><!--]--></p></div><!--]--></div><!--]--><!--]--><div class="fixed right-[-8vw] top-[35vh] opacity-20 z-[-1]"><img onerror="this.setAttribute(&#39;data-error&#39;, 1)" data-nuxt-img srcset="https://static.igem.wiki/teams/5358/icons/logo.png 1x, https://static.igem.wiki/teams/5358/icons/logo.png 2x" class="w-[30vw]" src="https://static.igem.wiki/teams/5358/icons/logo.png"></div></div><div class="text-center text-base mt-10 border-t-2 text-gray-300"><div class="flex flex-wrap justify-between mt-10 text-left mx-[1rem] lg:mx-[7.5rem]"><!--[--><div class="mx-5 mb-10"><span class="font-bold text-lg">Project</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/description" class="mt-1 cursor-pointer hover:underline">Description</a><a href="/bit/engineering" class="mt-1 cursor-pointer hover:underline">Engineering</a><a aria-current="page" href="/bit/safety" class="router-link-active router-link-exact-active mt-1 cursor-pointer hover:underline">Safety</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Web Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/experiment" class="mt-1 cursor-pointer hover:underline">Experiments</a><a href="/bit/result" class="mt-1 cursor-pointer hover:underline">Results</a><a href="/bit/parts" class="mt-1 cursor-pointer hover:underline">Notebook</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Dry Lab</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/hardware" class="mt-1 cursor-pointer hover:underline">Hardware</a><a href="/bit/model" class="mt-1 cursor-pointer hover:underline">Model</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Team</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/member" class="mt-1 cursor-pointer hover:underline">Members</a><a href="/bit/attributions" class="mt-1 cursor-pointer hover:underline">Attributions</a><a href="/bit/contribution" class="mt-1 cursor-pointer hover:underline">Contribution</a><!--]--></div></div><div class="mx-5 mb-10"><span class="font-bold text-lg">Human Practices</span><div class="flex flex-col flex-wrap mt-5"><!--[--><a href="/bit/education" class="mt-1 cursor-pointer hover:underline">Education</a><a href="/bit/human-practices" class="mt-1 cursor-pointer hover:underline">Integrated HP</a><!--]--></div></div><!--]--></div><div></div><div class="mt-[20vh] hidden"> Contact Us:   Instagram: igem_shanghaitech   Facebook: iGEM-ShanghaiTech   E-mail: igemshanghaitech@163.com <br> Bilibili: 上科大iGEM   Weibo: iGEM_ShanghaiTech   Xiaohongshu: iGEM_ShanghaiTech </div><div class="my-[10vh] pb-[8vh]"><div class="mb-1"> © 2024 - Content on this site is licensed under a <a href="https://creativecommons.org/licenses/by/4.0" rel="liscence" class="font-bold hover:underline text-white"> Creative Commons Attribution 4.0 International license </a> . </div><div> The repository used to create this website is available at <a href="https://gitlab.igem.org/2024/bit" rel="noopener noreferrer" class="font-bold hover:underline text-white"> gitlab.igem.org/2024/bit </a> . </div></div></div></div><!--]--></div><script type="application/json" id="__NUXT_DATA__" data-ssr="true" 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similarity index 84%
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@@ -1 +1 @@
-import{g as a,w,ae as y,i as D,u as H,aa as g,y as S,J as _,af as b,k as u}from"./entry.Cn1P5DRm.js";import k from"./ContentRenderer.DLx9YsgF.js";import q from"./ContentQuery.D8uFFL4u.js";import"./ContentRendererMarkdown.vue.DihjwSrL.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.DMaui_6Y.js";import"./query.DUXt7E8c.js";const d=(p,s=y())=>{const e=a(p),f=g();w(()=>a(p),(n=e)=>{if(!s.path||!n)return;const t=Object.assign({},(n==null?void 0:n.head)||{});t.meta=[...t.meta||[]],t.link=[...t.link||[]];const r=t.title||(n==null?void 0:n.title);r&&(t.title=r),f.public.content.host;const c=(t==null?void 0:t.description)||(n==null?void 0:n.description);c&&t.meta.filter(l=>l.name==="description").length===0&&t.meta.push({name:"description",content:c}),t!=null&&t.image||(n==null||n.image),D(()=>H(t))},{immediate:!0})},x=S({name:"ContentDoc",props:{tag:{type:String,required:!1,default:"div"},excerpt:{type:Boolean,default:!1},path:{type:String,required:!1,default:void 0},query:{type:Object,required:!1,default:void 0},head:{type:Boolean,required:!1,default:void 0}},render(p){const{contentHead:s}=g().public.content,e=_(),{tag:f,excerpt:m,path:n,query:t,head:r}=p,c=r===void 0?s:r,l={...t||{},path:n||(t==null?void 0:t.path)||b(y().path),find:"one"},C=(o,i)=>u("pre",null,JSON.stringify({message:"You should use slots with <ContentDoc>",slot:o,data:i},null,2));return u(q,l,{default:e!=null&&e.default?({data:o,refresh:i,isPartial:v})=>{var h;return c&&d(o),(h=e.default)==null?void 0:h.call(e,{doc:o,refresh:i,isPartial:v,excerpt:m,...this.$attrs})}:({data:o})=>(c&&d(o),u(k,{value:o,excerpt:m,tag:f,...this.$attrs},{empty:i=>e!=null&&e.empty?e.empty(i):C("default",o)})),empty:o=>{var i;return((i=e==null?void 0:e.empty)==null?void 0:i.call(e,o))||u("p",null,"Document is empty, overwrite this content with #empty slot in <ContentDoc>.")},"not-found":o=>{var i;return((i=e==null?void 0:e["not-found"])==null?void 0:i.call(e,o))||u("p",null,"Document not found, overwrite this content with #not-found slot in <ContentDoc>.")}})}}),I=x;export{I as default};
+import{g as a,w,ae as y,i as D,u as H,aa as g,y as S,J as _,af as b,k as u}from"./entry.ByxzK2uc.js";import k from"./ContentRenderer.CcHeFhnS.js";import q from"./ContentQuery.BkEWwCWH.js";import"./ContentRendererMarkdown.vue.BNzii3wK.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.CFiP-sCj.js";import"./query.HgboQeEc.js";const d=(p,s=y())=>{const e=a(p),f=g();w(()=>a(p),(n=e)=>{if(!s.path||!n)return;const t=Object.assign({},(n==null?void 0:n.head)||{});t.meta=[...t.meta||[]],t.link=[...t.link||[]];const r=t.title||(n==null?void 0:n.title);r&&(t.title=r),f.public.content.host;const c=(t==null?void 0:t.description)||(n==null?void 0:n.description);c&&t.meta.filter(l=>l.name==="description").length===0&&t.meta.push({name:"description",content:c}),t!=null&&t.image||(n==null||n.image),D(()=>H(t))},{immediate:!0})},x=S({name:"ContentDoc",props:{tag:{type:String,required:!1,default:"div"},excerpt:{type:Boolean,default:!1},path:{type:String,required:!1,default:void 0},query:{type:Object,required:!1,default:void 0},head:{type:Boolean,required:!1,default:void 0}},render(p){const{contentHead:s}=g().public.content,e=_(),{tag:f,excerpt:m,path:n,query:t,head:r}=p,c=r===void 0?s:r,l={...t||{},path:n||(t==null?void 0:t.path)||b(y().path),find:"one"},C=(o,i)=>u("pre",null,JSON.stringify({message:"You should use slots with <ContentDoc>",slot:o,data:i},null,2));return u(q,l,{default:e!=null&&e.default?({data:o,refresh:i,isPartial:v})=>{var h;return c&&d(o),(h=e.default)==null?void 0:h.call(e,{doc:o,refresh:i,isPartial:v,excerpt:m,...this.$attrs})}:({data:o})=>(c&&d(o),u(k,{value:o,excerpt:m,tag:f,...this.$attrs},{empty:i=>e!=null&&e.empty?e.empty(i):C("default",o)})),empty:o=>{var i;return((i=e==null?void 0:e.empty)==null?void 0:i.call(e,o))||u("p",null,"Document is empty, overwrite this content with #empty slot in <ContentDoc>.")},"not-found":o=>{var i;return((i=e==null?void 0:e["not-found"])==null?void 0:i.call(e,o))||u("p",null,"Document not found, overwrite this content with #not-found slot in <ContentDoc>.")}})}}),I=x;export{I as default};
diff --git a/.output/public/static/ContentList.ChPK7_ni.js b/.output/public/static/ContentList.D_khWugl.js
similarity index 78%
rename from .output/public/static/ContentList.ChPK7_ni.js
rename to .output/public/static/ContentList.D_khWugl.js
index c65805209512fcadc0de58dad4d1393f7801f73d..4299bea1162b594c603151d4caea10d2270efee9 100644
--- a/.output/public/static/ContentList.ChPK7_ni.js
+++ b/.output/public/static/ContentList.D_khWugl.js
@@ -1 +1 @@
-import d from"./ContentQuery.D8uFFL4u.js";import{y as c,J as l,k as f}from"./entry.Cn1P5DRm.js";import"./query.DUXt7E8c.js";import"./preview.DMaui_6Y.js";const u=(r,t)=>f("pre",null,JSON.stringify({message:"You should use slots with <ContentList>",slot:r,data:t},null,2)),y=c({name:"ContentList",props:{path:{type:String,required:!1,default:void 0},query:{type:Object,required:!1,default:void 0}},render(r){const t=l(),{path:p,query:a}=r,m={...a||{},path:p||(a==null?void 0:a.path)||"/"};return f(d,m,{default:t!=null&&t.default?({data:e,refresh:o,isPartial:n})=>t.default({list:e,refresh:o,isPartial:n,...this.$attrs}):e=>u("default",e.data),empty:e=>t!=null&&t.empty?t.empty(e):u("default",e==null?void 0:e.data),"not-found":e=>{var o;return t!=null&&t["not-found"]?(o=t==null?void 0:t["not-found"])==null?void 0:o.call(t,e):u("not-found",e==null?void 0:e.data)}})}}),L=y;export{L as default};
+import d from"./ContentQuery.BkEWwCWH.js";import{y as c,J as l,k as f}from"./entry.ByxzK2uc.js";import"./query.HgboQeEc.js";import"./preview.CFiP-sCj.js";const u=(r,t)=>f("pre",null,JSON.stringify({message:"You should use slots with <ContentList>",slot:r,data:t},null,2)),y=c({name:"ContentList",props:{path:{type:String,required:!1,default:void 0},query:{type:Object,required:!1,default:void 0}},render(r){const t=l(),{path:p,query:a}=r,m={...a||{},path:p||(a==null?void 0:a.path)||"/"};return f(d,m,{default:t!=null&&t.default?({data:e,refresh:o,isPartial:n})=>t.default({list:e,refresh:o,isPartial:n,...this.$attrs}):e=>u("default",e.data),empty:e=>t!=null&&t.empty?t.empty(e):u("default",e==null?void 0:e.data),"not-found":e=>{var o;return t!=null&&t["not-found"]?(o=t==null?void 0:t["not-found"])==null?void 0:o.call(t,e):u("not-found",e==null?void 0:e.data)}})}}),L=y;export{L as default};
diff --git a/.output/public/static/ContentNavigation.Dmk15lqZ.js b/.output/public/static/ContentNavigation.B1gOh5Mb.js
similarity index 74%
rename from .output/public/static/ContentNavigation.Dmk15lqZ.js
rename to .output/public/static/ContentNavigation.B1gOh5Mb.js
index c57d4b977b866ab29b054b48b840262dfb557a4a..9a3ce737e190909d738133c43e916287d17a51e2 100644
--- a/.output/public/static/ContentNavigation.Dmk15lqZ.js
+++ b/.output/public/static/ContentNavigation.B1gOh5Mb.js
@@ -1,7 +1,7 @@
-import{q as f,w as m,e as v,s as g,j as d,u as l,a as h}from"./query.DUXt7E8c.js";import{aa as _,ag as p,ah as y,y as C,a3 as w,m as P,ai as $,J as N,k as r}from"./entry.Cn1P5DRm.js";import{u as j}from"./preview.DMaui_6Y.js";import{_ as T}from"./nuxt-link.CLgB-HXB.js";const D=async e=>{const{content:t}=_().public;typeof(e==null?void 0:e.params)!="function"&&(e=f(e));const a=e.params(),s=t.experimental.stripQueryParameters?m(`/navigation/${`${p(a)}.${t.integrity}`}/${v(a)}.json`):m(`/navigation/${p(a)}.${t.integrity}.json`);if(g())return(await y(()=>import("./client-db.BgUG8l1f.js"),__vite__mapDeps([0,1,2,3,4,5]),import.meta.url).then(o=>o.generateNavigation))(a);const n=await $fetch(s,{method:"GET",responseType:"json",params:t.experimental.stripQueryParameters?void 0:{_params:d(a),previewToken:j().getPreviewToken()}});if(typeof n=="string"&&n.startsWith("<!DOCTYPE html>"))throw new Error("Not found");return n},E=C({name:"ContentNavigation",props:{query:{type:Object,required:!1,default:void 0}},async setup(e){const{query:t}=w(e),a=P(()=>{var n;return typeof((n=t.value)==null?void 0:n.params)=="function"?t.value.params():t.value});if(!a.value&&$("dd-navigation").value){const{navigation:n}=l();return{navigation:n}}const{data:s}=await h(`content-navigation-${p(a.value)}`,()=>D(a.value));return{navigation:s}},render(e){const t=N(),{navigation:a}=e,s=o=>r(T,{to:o._path},()=>o.title),n=(o,u)=>r("ul",u?{"data-level":u}:null,o.map(i=>i.children?r("li",null,[s(i),n(i.children,u+1)]):r("li",null,s(i)))),c=o=>n(o,0);return t!=null&&t.default?t.default({navigation:a,...this.$attrs}):c(a)}}),Q=E;export{Q as default};
+import{q as f,w as m,e as v,s as g,j as d,u as l,a as h}from"./query.HgboQeEc.js";import{aa as _,ag as p,ah as y,y as C,a3 as w,m as P,ai as $,J as N,k as r}from"./entry.ByxzK2uc.js";import{u as j}from"./preview.CFiP-sCj.js";import{_ as T}from"./nuxt-link.B-U_8utk.js";const D=async e=>{const{content:t}=_().public;typeof(e==null?void 0:e.params)!="function"&&(e=f(e));const a=e.params(),s=t.experimental.stripQueryParameters?m(`/navigation/${`${p(a)}.${t.integrity}`}/${v(a)}.json`):m(`/navigation/${p(a)}.${t.integrity}.json`);if(g())return(await y(()=>import("./client-db.BXhUscuN.js"),__vite__mapDeps([0,1,2,3,4,5]),import.meta.url).then(o=>o.generateNavigation))(a);const n=await $fetch(s,{method:"GET",responseType:"json",params:t.experimental.stripQueryParameters?void 0:{_params:d(a),previewToken:j().getPreviewToken()}});if(typeof n=="string"&&n.startsWith("<!DOCTYPE html>"))throw new Error("Not found");return n},E=C({name:"ContentNavigation",props:{query:{type:Object,required:!1,default:void 0}},async setup(e){const{query:t}=w(e),a=P(()=>{var n;return typeof((n=t.value)==null?void 0:n.params)=="function"?t.value.params():t.value});if(!a.value&&$("dd-navigation").value){const{navigation:n}=l();return{navigation:n}}const{data:s}=await h(`content-navigation-${p(a.value)}`,()=>D(a.value));return{navigation:s}},render(e){const t=N(),{navigation:a}=e,s=o=>r(T,{to:o._path},()=>o.title),n=(o,u)=>r("ul",u?{"data-level":u}:null,o.map(i=>i.children?r("li",null,[s(i),n(i.children,u+1)]):r("li",null,s(i)))),c=o=>n(o,0);return t!=null&&t.default?t.default({navigation:a,...this.$attrs}):c(a)}}),Q=E;export{Q as default};
 function __vite__mapDeps(indexes) {
   if (!__vite__mapDeps.viteFileDeps) {
-    __vite__mapDeps.viteFileDeps = ["./client-db.BgUG8l1f.js","./entry.Cn1P5DRm.js","./entry.CpJXJ09x.css","./query.DUXt7E8c.js","./preview.DMaui_6Y.js","./index.C-v3KzvZ.js"]
+    __vite__mapDeps.viteFileDeps = ["./client-db.BXhUscuN.js","./entry.ByxzK2uc.js","./entry.DCK6esyZ.css","./query.HgboQeEc.js","./preview.CFiP-sCj.js","./index.C-v3KzvZ.js"]
   }
   return indexes.map((i) => __vite__mapDeps.viteFileDeps[i])
 }
diff --git a/.output/public/static/ContentQuery.D8uFFL4u.js b/.output/public/static/ContentQuery.BkEWwCWH.js
similarity index 92%
rename from .output/public/static/ContentQuery.D8uFFL4u.js
rename to .output/public/static/ContentQuery.BkEWwCWH.js
index a2b801aa22c58c5dfdfc3b50c688ddb2e72662e0..b31e346f800f455f270b1fad6c3ce3013ff7df48 100644
--- a/.output/public/static/ContentQuery.D8uFFL4u.js
+++ b/.output/public/static/ContentQuery.BkEWwCWH.js
@@ -1 +1 @@
-import{a as g,q as m}from"./query.DUXt7E8c.js";import{y as C,a3 as S,m as b,aa as k,w as _,ag as A,J as N,k as O}from"./entry.Cn1P5DRm.js";import"./preview.DMaui_6Y.js";const Q=C({name:"ContentQuery",props:{path:{type:String,required:!1,default:void 0},only:{type:Array,required:!1,default:void 0},without:{type:Array,required:!1,default:void 0},where:{type:Object,required:!1,default:void 0},sort:{type:Object,required:!1,default:void 0},limit:{type:Number,required:!1,default:void 0},skip:{type:Number,required:!1,default:void 0},locale:{type:String,required:!1,default:void 0},find:{type:String,required:!1,default:void 0}},async setup(a){const{path:t,only:r,without:o,where:u,sort:l,limit:f,skip:d,locale:s,find:p}=S(a),h=b(()=>{var e;return(e=t.value)==null?void 0:e.includes("/_")}),y=!k().public.content.experimental.advanceQuery;_(()=>a,()=>n(),{deep:!0});const i=e=>y?e!=null&&e.surround?e.surround:e!=null&&e._id||Array.isArray(e)?e:e==null?void 0:e.result:e.result,{data:v,refresh:n}=await g(`content-query-${A(a)}`,()=>{let e;return t.value?e=m(t.value):e=m(),r.value&&(e=e.only(r.value)),o.value&&(e=e.without(o.value)),u.value&&(e=e.where(u.value)),l.value&&(e=e.sort(l.value)),f.value&&(e=e.limit(f.value)),d.value&&(e=e.skip(d.value)),s.value&&(e=e.where({_locale:s.value})),p.value==="one"?e.findOne().then(i):p.value==="surround"?t.value?y?e.findSurround(t.value):e.withSurround(t.value).findOne().then(i):(console.warn("[Content] Surround queries requires `path` prop to be set."),console.warn("[Content] Query without `path` will return regular `find()` results."),e.find().then(i)):e.find().then(i)});return{isPartial:h,data:v,refresh:n}},render(a){var c;const t=N(),{data:r,refresh:o,isPartial:u,path:l,only:f,without:d,where:s,sort:p,limit:h,skip:y,locale:i,find:v}=a,n={path:l,only:f,without:d,where:s,sort:p,limit:h,skip:y,locale:i,find:v};if(n.find==="one"){if(!r&&(t!=null&&t["not-found"]))return t["not-found"]({props:n,...this.$attrs});if(t!=null&&t.empty&&(r==null?void 0:r._type)==="markdown"&&!((c=r==null?void 0:r.body)!=null&&c.children.length))return t.empty({props:n,...this.$attrs})}else if((!r||!r.length)&&t!=null&&t["not-found"])return t["not-found"]({props:n,...this.$attrs});return t!=null&&t.default?t.default({data:r,refresh:o,isPartial:u,props:n,...this.$attrs}):((w,q)=>O("pre",null,JSON.stringify({message:"You should use slots with <ContentQuery>!",slot:w,data:q},null,2)))("default",{data:r,props:n,isPartial:u})}}),x=Q;export{x as default};
+import{a as g,q as m}from"./query.HgboQeEc.js";import{y as C,a3 as S,m as b,aa as k,w as _,ag as A,J as N,k as O}from"./entry.ByxzK2uc.js";import"./preview.CFiP-sCj.js";const Q=C({name:"ContentQuery",props:{path:{type:String,required:!1,default:void 0},only:{type:Array,required:!1,default:void 0},without:{type:Array,required:!1,default:void 0},where:{type:Object,required:!1,default:void 0},sort:{type:Object,required:!1,default:void 0},limit:{type:Number,required:!1,default:void 0},skip:{type:Number,required:!1,default:void 0},locale:{type:String,required:!1,default:void 0},find:{type:String,required:!1,default:void 0}},async setup(a){const{path:t,only:r,without:o,where:u,sort:l,limit:f,skip:d,locale:s,find:p}=S(a),h=b(()=>{var e;return(e=t.value)==null?void 0:e.includes("/_")}),y=!k().public.content.experimental.advanceQuery;_(()=>a,()=>n(),{deep:!0});const i=e=>y?e!=null&&e.surround?e.surround:e!=null&&e._id||Array.isArray(e)?e:e==null?void 0:e.result:e.result,{data:v,refresh:n}=await g(`content-query-${A(a)}`,()=>{let e;return t.value?e=m(t.value):e=m(),r.value&&(e=e.only(r.value)),o.value&&(e=e.without(o.value)),u.value&&(e=e.where(u.value)),l.value&&(e=e.sort(l.value)),f.value&&(e=e.limit(f.value)),d.value&&(e=e.skip(d.value)),s.value&&(e=e.where({_locale:s.value})),p.value==="one"?e.findOne().then(i):p.value==="surround"?t.value?y?e.findSurround(t.value):e.withSurround(t.value).findOne().then(i):(console.warn("[Content] Surround queries requires `path` prop to be set."),console.warn("[Content] Query without `path` will return regular `find()` results."),e.find().then(i)):e.find().then(i)});return{isPartial:h,data:v,refresh:n}},render(a){var c;const t=N(),{data:r,refresh:o,isPartial:u,path:l,only:f,without:d,where:s,sort:p,limit:h,skip:y,locale:i,find:v}=a,n={path:l,only:f,without:d,where:s,sort:p,limit:h,skip:y,locale:i,find:v};if(n.find==="one"){if(!r&&(t!=null&&t["not-found"]))return t["not-found"]({props:n,...this.$attrs});if(t!=null&&t.empty&&(r==null?void 0:r._type)==="markdown"&&!((c=r==null?void 0:r.body)!=null&&c.children.length))return t.empty({props:n,...this.$attrs})}else if((!r||!r.length)&&t!=null&&t["not-found"])return t["not-found"]({props:n,...this.$attrs});return t!=null&&t.default?t.default({data:r,refresh:o,isPartial:u,props:n,...this.$attrs}):((w,q)=>O("pre",null,JSON.stringify({message:"You should use slots with <ContentQuery>!",slot:w,data:q},null,2)))("default",{data:r,props:n,isPartial:u})}}),x=Q;export{x as default};
diff --git a/.output/public/static/ContentRenderer.DLx9YsgF.js b/.output/public/static/ContentRenderer.CcHeFhnS.js
similarity index 85%
rename from .output/public/static/ContentRenderer.DLx9YsgF.js
rename to .output/public/static/ContentRenderer.CcHeFhnS.js
index 0d7b64531b6d96c6631a11c118dae236e92cd2f8..19ed92d0956beb92358dd2eca25ca4e5c08ee08e 100644
--- a/.output/public/static/ContentRenderer.DLx9YsgF.js
+++ b/.output/public/static/ContentRenderer.CcHeFhnS.js
@@ -1 +1 @@
-import{_ as f}from"./ContentRendererMarkdown.vue.DihjwSrL.js";import{y as l,w as s,J as d,k as c}from"./entry.Cn1P5DRm.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.DMaui_6Y.js";const _=l({name:"ContentRenderer",props:{value:{type:Object,required:!1,default:()=>({})},excerpt:{type:Boolean,default:!1},tag:{type:String,default:"div"}},setup(t){s(()=>t.excerpt,n=>{var e,a,i;n&&!((e=t.value)!=null&&e.excerpt)&&(console.warn(`No excerpt found for document content/${(a=t==null?void 0:t.value)==null?void 0:a._path}.${(i=t==null?void 0:t.value)==null?void 0:i._extension}!`),console.warn("Make sure to use <!--more--> in your content if you want to use excerpt feature."))},{immediate:!0})},render(t){var u,o;const n=d(),{value:e,excerpt:a,tag:i}=t,r=a?e==null?void 0:e.excerpt:e==null?void 0:e.body;return!((u=r==null?void 0:r.children)!=null&&u.length)&&(n!=null&&n.empty)?n.empty({value:e,excerpt:a,tag:i,...this.$attrs}):n!=null&&n.default?n.default({value:e,excerpt:a,tag:i,...this.$attrs}):(r==null?void 0:r.type)==="root"&&((o=r==null?void 0:r.children)!=null&&o.length)?c(f,{value:e,excerpt:a,tag:i,...this.$attrs}):c("pre",null,JSON.stringify({message:"You should use slots with <ContentRenderer>",value:e,excerpt:a,tag:i},null,2))}});export{_ as default};
+import{_ as f}from"./ContentRendererMarkdown.vue.BNzii3wK.js";import{y as l,w as s,J as d,k as c}from"./entry.ByxzK2uc.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.CFiP-sCj.js";const _=l({name:"ContentRenderer",props:{value:{type:Object,required:!1,default:()=>({})},excerpt:{type:Boolean,default:!1},tag:{type:String,default:"div"}},setup(t){s(()=>t.excerpt,n=>{var e,a,i;n&&!((e=t.value)!=null&&e.excerpt)&&(console.warn(`No excerpt found for document content/${(a=t==null?void 0:t.value)==null?void 0:a._path}.${(i=t==null?void 0:t.value)==null?void 0:i._extension}!`),console.warn("Make sure to use <!--more--> in your content if you want to use excerpt feature."))},{immediate:!0})},render(t){var u,o;const n=d(),{value:e,excerpt:a,tag:i}=t,r=a?e==null?void 0:e.excerpt:e==null?void 0:e.body;return!((u=r==null?void 0:r.children)!=null&&u.length)&&(n!=null&&n.empty)?n.empty({value:e,excerpt:a,tag:i,...this.$attrs}):n!=null&&n.default?n.default({value:e,excerpt:a,tag:i,...this.$attrs}):(r==null?void 0:r.type)==="root"&&((o=r==null?void 0:r.children)!=null&&o.length)?c(f,{value:e,excerpt:a,tag:i,...this.$attrs}):c("pre",null,JSON.stringify({message:"You should use slots with <ContentRenderer>",value:e,excerpt:a,tag:i},null,2))}});export{_ as default};
diff --git a/.output/public/static/ContentRendererMarkdown.DT7Eq5Ii.js b/.output/public/static/ContentRendererMarkdown.DT7Eq5Ii.js
new file mode 100644
index 0000000000000000000000000000000000000000..fd78a0fdffdd818c23eda593ab97472e66dbc38b
--- /dev/null
+++ b/.output/public/static/ContentRendererMarkdown.DT7Eq5Ii.js
@@ -0,0 +1 @@
+import{_ as o}from"./ContentRendererMarkdown.vue.BNzii3wK.js";import"./entry.ByxzK2uc.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.CFiP-sCj.js";export{o as default};
diff --git a/.output/public/static/ContentRendererMarkdown.DwwqHW6g.js b/.output/public/static/ContentRendererMarkdown.DwwqHW6g.js
deleted file mode 100644
index 756029074ea37d9df66e8ceeedb82c9039449f80..0000000000000000000000000000000000000000
--- a/.output/public/static/ContentRendererMarkdown.DwwqHW6g.js
+++ /dev/null
@@ -1 +0,0 @@
-import{_ as o}from"./ContentRendererMarkdown.vue.DihjwSrL.js";import"./entry.Cn1P5DRm.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.DMaui_6Y.js";export{o as default};
diff --git a/.output/public/static/ContentRendererMarkdown.vue.DihjwSrL.js b/.output/public/static/ContentRendererMarkdown.vue.BNzii3wK.js
similarity index 99%
rename from .output/public/static/ContentRendererMarkdown.vue.DihjwSrL.js
rename to .output/public/static/ContentRendererMarkdown.vue.BNzii3wK.js
index 01129324ac392f6e4802612a9c046216cd04bbed..cff7ee4d49a9fa51e4aae0551983b0ab2e24ec3e 100644
--- a/.output/public/static/ContentRendererMarkdown.vue.DihjwSrL.js
+++ b/.output/public/static/ContentRendererMarkdown.vue.BNzii3wK.js
@@ -1 +1 @@
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diff --git a/.output/public/static/ContentSlot.B3nA0QNu.js b/.output/public/static/ContentSlot.zbSk5Aai.js
similarity index 92%
rename from .output/public/static/ContentSlot.B3nA0QNu.js
rename to .output/public/static/ContentSlot.zbSk5Aai.js
index 5f4b68443a2a350cb5a57ae309bdcf0133e3507c..5047887b844456ee0b89dc4c11fde56f201ea61e 100644
--- a/.output/public/static/ContentSlot.B3nA0QNu.js
+++ b/.output/public/static/ContentSlot.zbSk5Aai.js
@@ -1 +1 @@
-import{f as i}from"./node.Dnd51l0P.js";import{y as u,J as f,m as c,k as a,L as d}from"./entry.Cn1P5DRm.js";const p=u({name:"MDCSlot",functional:!0,props:{name:{type:String,default:"default"},unwrap:{type:[Boolean,String],default:!1},use:{type:Function,default:void 0}},setup(t){const{parent:s}=d(),{default:o}=f(),r=c(()=>typeof t.unwrap=="string"?t.unwrap.split(" "):["*"]);return{fallbackSlot:o,tags:r,parent:s}},render({use:t,unwrap:s,fallbackSlot:o,tags:r,parent:e}){var l;try{let n=t;return typeof t=="string"&&(n=(e==null?void 0:e.slots[t])||((l=e==null?void 0:e.parent)==null?void 0:l.slots[t]),console.warn(`Please set :use="$slots.${t}" in <MDCSlot> component to enable reactivity`)),n?s?i(n(),r):[n()]:o?o():a("div")}catch{return a("div")}}}),g=u({props:{use:{type:Function,default:void 0},unwrap:{type:[Boolean,String],default:!1}},render(t){return a(p,t)}});export{g as default};
+import{f as i}from"./node.Dnd51l0P.js";import{y as u,J as f,m as c,k as a,L as d}from"./entry.ByxzK2uc.js";const p=u({name:"MDCSlot",functional:!0,props:{name:{type:String,default:"default"},unwrap:{type:[Boolean,String],default:!1},use:{type:Function,default:void 0}},setup(t){const{parent:s}=d(),{default:o}=f(),r=c(()=>typeof t.unwrap=="string"?t.unwrap.split(" "):["*"]);return{fallbackSlot:o,tags:r,parent:s}},render({use:t,unwrap:s,fallbackSlot:o,tags:r,parent:e}){var l;try{let n=t;return typeof t=="string"&&(n=(e==null?void 0:e.slots[t])||((l=e==null?void 0:e.parent)==null?void 0:l.slots[t]),console.warn(`Please set :use="$slots.${t}" in <MDCSlot> component to enable reactivity`)),n?s?i(n(),r):[n()]:o?o():a("div")}catch{return a("div")}}}),g=u({props:{use:{type:Function,default:void 0},unwrap:{type:[Boolean,String],default:!1}},render(t){return a(p,t)}});export{g as default};
diff --git a/.output/public/static/DocumentDrivenEmpty.D3YaS058.js b/.output/public/static/DocumentDrivenEmpty.BegFdCt-.js
similarity index 77%
rename from .output/public/static/DocumentDrivenEmpty.D3YaS058.js
rename to .output/public/static/DocumentDrivenEmpty.BegFdCt-.js
index 01bccb9d7624de99c1e3089cb6eb523767e7b776..a42d56efcdb7be2d6b4c9f78a5940a9b761a51f7 100644
--- a/.output/public/static/DocumentDrivenEmpty.D3YaS058.js
+++ b/.output/public/static/DocumentDrivenEmpty.BegFdCt-.js
@@ -1 +1 @@
-import{y as n,k as e}from"./entry.Cn1P5DRm.js";const r=n({name:"DocumentDrivenEmpty",props:{value:{type:Object,required:!0}},render({value:t}){return e("div",void 0,[e("p","Document is empty"),e("p",`Add content to it by opening ${t._source}/${t._file} file.`)])}});export{r as default};
+import{y as n,k as e}from"./entry.ByxzK2uc.js";const r=n({name:"DocumentDrivenEmpty",props:{value:{type:Object,required:!0}},render({value:t}){return e("div",void 0,[e("p","Document is empty"),e("p",`Add content to it by opening ${t._source}/${t._file} file.`)])}});export{r as default};
diff --git a/.output/public/static/DocumentDrivenNotFound.XK9YseyS.js b/.output/public/static/DocumentDrivenNotFound.x0qNkzO0.js
similarity index 59%
rename from .output/public/static/DocumentDrivenNotFound.XK9YseyS.js
rename to .output/public/static/DocumentDrivenNotFound.x0qNkzO0.js
index f62ce9407fb38ba4399619854268c18ae73793e1..bab8d22802cc5af31f6628b9efd71c121418c257 100644
--- a/.output/public/static/DocumentDrivenNotFound.XK9YseyS.js
+++ b/.output/public/static/DocumentDrivenNotFound.x0qNkzO0.js
@@ -1 +1 @@
-import{y as n,k as e}from"./entry.Cn1P5DRm.js";const t=n({name:"DocumentDrivenNotFound",render(){return e("div","Document not found")}});export{t as default};
+import{y as n,k as e}from"./entry.ByxzK2uc.js";const t=n({name:"DocumentDrivenNotFound",render(){return e("div","Document not found")}});export{t as default};
diff --git a/.output/public/static/Markdown.BtCac_SB.js b/.output/public/static/Markdown.DJL8rxEJ.js
similarity index 64%
rename from .output/public/static/Markdown.BtCac_SB.js
rename to .output/public/static/Markdown.DJL8rxEJ.js
index 1e4819c1db41f3e9ae06ec925db138ec0190f2bf..737faa70a6a40ed4f7f568dfc217acaf42b5f5e6 100644
--- a/.output/public/static/Markdown.BtCac_SB.js
+++ b/.output/public/static/Markdown.DJL8rxEJ.js
@@ -1 +1 @@
-import s from"./ContentSlot.B3nA0QNu.js";import{y as o,J as m,m as u,L as f}from"./entry.Cn1P5DRm.js";import"./node.Dnd51l0P.js";const l=o({name:"Markdown",extends:s,setup(t){const{parent:e}=f(),{between:n,default:a}=m(),r=u(()=>typeof t.unwrap=="string"?t.unwrap.split(" "):["*"]);return{fallbackSlot:a,tags:r,between:n,parent:e}}});export{l as default};
+import s from"./ContentSlot.zbSk5Aai.js";import{y as o,J as m,m as u,L as f}from"./entry.ByxzK2uc.js";import"./node.Dnd51l0P.js";const l=o({name:"Markdown",extends:s,setup(t){const{parent:e}=f(),{between:n,default:a}=m(),r=u(()=>typeof t.unwrap=="string"?t.unwrap.split(" "):["*"]);return{fallbackSlot:a,tags:r,between:n,parent:e}}});export{l as default};
diff --git a/.output/public/static/MdcWrap.DkLJEMKo.js b/.output/public/static/MdcWrap.Bv1YeLc5.js
similarity index 80%
rename from .output/public/static/MdcWrap.DkLJEMKo.js
rename to .output/public/static/MdcWrap.Bv1YeLc5.js
index 4885b68362f99907d88a305e96c14183a020e6cd..e8c51ea579e9a1d80c29b34db7b867b8db8f6947 100644
--- a/.output/public/static/MdcWrap.DkLJEMKo.js
+++ b/.output/public/static/MdcWrap.Bv1YeLc5.js
@@ -1 +1 @@
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+import{f as a}from"./node.Dnd51l0P.js";import{A as n,_ as f,o as d,c as l}from"./entry.ByxzK2uc.js";const o=(r,e,t,...c)=>r[e]?n({...r,[e]:()=>a(r[e](),t==null?void 0:t.unwrap)},e,t,...c):n(r,e,t,...c),u={};function _(r,e){return d(),l("div",null,[o(r.$slots,"default")])}const m=f(u,[["render",_]]);export{m as default};
diff --git a/.output/public/static/NuxtImg.vue.CxZEoKQV.js b/.output/public/static/NuxtImg.vue.f86GhESM.js
similarity index 99%
rename from .output/public/static/NuxtImg.vue.CxZEoKQV.js
rename to .output/public/static/NuxtImg.vue.f86GhESM.js
index d92deabb0418dce63931223c970ccee8a66881c3..961100d9069ba3c02362ae723fe9b0c916930024 100644
--- a/.output/public/static/NuxtImg.vue.CxZEoKQV.js
+++ b/.output/public/static/NuxtImg.vue.f86GhESM.js
@@ -1 +1 @@
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+import{ax as z,ao as R,ap as q,ay as D,az as P,aa as O,aA as $,aB as U,ad as E,m as v,y as C,a6 as G,r as I,b as L,o as T,c as F,a5 as V,g as W}from"./entry.ByxzK2uc.js";async function J(e,t){return await Y(t).catch(r=>(console.error("Failed to get image meta for "+t,r+""),{width:0,height:0,ratio:0}))}async function Y(e){if(typeof Image>"u")throw new TypeError("Image not supported");return new Promise((t,i)=>{const r=new Image;r.onload=()=>{const s={width:r.width,height:r.height,ratio:r.width/r.height};t(s)},r.onerror=s=>i(s),r.src=e})}function j(e){return t=>t?e[t]||t:e.missingValue}function N({formatter:e,keyMap:t,joinWith:i="/",valueMap:r}={}){e||(e=(n,o)=>`${n}=${o}`),t&&typeof t!="function"&&(t=j(t));const s=r||{};return Object.keys(s).forEach(n=>{typeof s[n]!="function"&&(s[n]=j(s[n]))}),(n={})=>Object.entries(n).filter(([a,d])=>typeof d<"u").map(([a,d])=>{const l=s[a];return typeof l=="function"&&(d=l(n[a])),a=typeof t=="function"?t(a):a,e(a,d)}).join(i)}function 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diff --git a/.output/public/static/ProseA.X_Ks2iIv.js b/.output/public/static/ProseA.Bk64ojI8.js
similarity index 67%
rename from .output/public/static/ProseA.X_Ks2iIv.js
rename to .output/public/static/ProseA.Bk64ojI8.js
index 03b270583860c95e8617ad7c622731826ade56a8..07ed57462e6df1f9a5a455cdd79e66246f7ab8e2 100644
--- a/.output/public/static/ProseA.X_Ks2iIv.js
+++ b/.output/public/static/ProseA.Bk64ojI8.js
@@ -1 +1 @@
-import{_ as a}from"./nuxt-link.CLgB-HXB.js";import{y as o,o as n,s,e as f,A as u}from"./entry.Cn1P5DRm.js";const l=o({__name:"ProseA",props:{href:{type:String,default:""},target:{type:String,default:void 0,required:!1}},setup(e){return(t,_)=>{const r=a;return n(),s(r,{href:e.href,target:e.target},{default:f(()=>[u(t.$slots,"default")]),_:3},8,["href","target"])}}});export{l as default};
+import{_ as a}from"./nuxt-link.B-U_8utk.js";import{y as o,o as n,s,e as f,A as u}from"./entry.ByxzK2uc.js";const l=o({__name:"ProseA",props:{href:{type:String,default:""},target:{type:String,default:void 0,required:!1}},setup(e){return(t,_)=>{const r=a;return n(),s(r,{href:e.href,target:e.target},{default:f(()=>[u(t.$slots,"default")]),_:3},8,["href","target"])}}});export{l as default};
diff --git a/.output/public/static/ProseBlockquote.Grm8PA6Y.js b/.output/public/static/ProseBlockquote.BUku3Oup.js
similarity index 66%
rename from .output/public/static/ProseBlockquote.Grm8PA6Y.js
rename to .output/public/static/ProseBlockquote.BUku3Oup.js
index 41b5ec482e0b30d551bf137e60c76b3de54f5d1a..1545a2b7477fad7cccad9ee5ff095263e20b1bf5 100644
--- a/.output/public/static/ProseBlockquote.Grm8PA6Y.js
+++ b/.output/public/static/ProseBlockquote.BUku3Oup.js
@@ -1 +1 @@
-import{_ as o,o as t,c,A as r}from"./entry.Cn1P5DRm.js";const s={};function n(e,l){return t(),c("blockquote",null,[r(e.$slots,"default")])}const _=o(s,[["render",n]]);export{_ as default};
+import{_ as o,o as t,c,A as r}from"./entry.ByxzK2uc.js";const s={};function n(e,l){return t(),c("blockquote",null,[r(e.$slots,"default")])}const _=o(s,[["render",n]]);export{_ as default};
diff --git a/.output/public/static/ProseCode.BKuaT4bp.js b/.output/public/static/ProseCode.BKuaT4bp.js
new file mode 100644
index 0000000000000000000000000000000000000000..fb1b7a04fa3c8e8562540d3f7447d6339b42d51a
--- /dev/null
+++ b/.output/public/static/ProseCode.BKuaT4bp.js
@@ -0,0 +1 @@
+import{_ as m}from"./ProseCode.vue.6rThu75v.js";import"./entry.ByxzK2uc.js";export{m as default};
diff --git a/.output/public/static/ProseCode.Be9Wcu4F.js b/.output/public/static/ProseCode.Be9Wcu4F.js
deleted file mode 100644
index 733483771e185ed048727c1861b2c189a0dd1ac6..0000000000000000000000000000000000000000
--- a/.output/public/static/ProseCode.Be9Wcu4F.js
+++ /dev/null
@@ -1 +0,0 @@
-import{_ as m}from"./ProseCode.vue.BamOH8Eq.js";import"./entry.Cn1P5DRm.js";export{m as default};
diff --git a/.output/public/static/ProseCode.vue.BamOH8Eq.js b/.output/public/static/ProseCode.vue.6rThu75v.js
similarity index 80%
rename from .output/public/static/ProseCode.vue.BamOH8Eq.js
rename to .output/public/static/ProseCode.vue.6rThu75v.js
index afbbd27dad93ab60501536b459cadcb41dae8d95..9265e99db156a3ba76e4b43f198597e6f9ae11f8 100644
--- a/.output/public/static/ProseCode.vue.BamOH8Eq.js
+++ b/.output/public/static/ProseCode.vue.6rThu75v.js
@@ -1 +1 @@
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+import{y as t,A as n}from"./entry.ByxzK2uc.js";const o=t({__name:"ProseCode",props:{code:{type:String,default:""},language:{type:String,default:null},filename:{type:String,default:null},highlights:{type:Array,default:()=>[]},meta:{type:String,default:null}},setup(r){return(e,a)=>n(e.$slots,"default")}});export{o as _};
diff --git a/.output/public/static/ProseCodeInline.CdKinuUb.js b/.output/public/static/ProseCodeInline.DAIK7XPu.js
similarity index 65%
rename from .output/public/static/ProseCodeInline.CdKinuUb.js
rename to .output/public/static/ProseCodeInline.DAIK7XPu.js
index ddae0dbb0acedd0470d15907a8d68b879342d379..986f26a98a20c466889e0c6bad199101a425ca30 100644
--- a/.output/public/static/ProseCodeInline.CdKinuUb.js
+++ b/.output/public/static/ProseCodeInline.DAIK7XPu.js
@@ -1 +1 @@
-import{_ as o,o as n,c as r,A as c}from"./entry.Cn1P5DRm.js";const s={};function t(e,a){return n(),r("code",null,[c(e.$slots,"default")])}const _=o(s,[["render",t]]);export{_ as default};
+import{_ as o,o as n,c as r,A as c}from"./entry.ByxzK2uc.js";const s={};function t(e,a){return n(),r("code",null,[c(e.$slots,"default")])}const _=o(s,[["render",t]]);export{_ as default};
diff --git a/.output/public/static/ProseEm.DGmvzXhY.js b/.output/public/static/ProseEm.DZbJLlHd.js
similarity index 65%
rename from .output/public/static/ProseEm.DGmvzXhY.js
rename to .output/public/static/ProseEm.DZbJLlHd.js
index b0d209c4ffd245bc153829a595a42b4d4599a2e9..4d2af89866178f65c54b258b3ef06e7c30e35d21 100644
--- a/.output/public/static/ProseEm.DGmvzXhY.js
+++ b/.output/public/static/ProseEm.DZbJLlHd.js
@@ -1 +1 @@
-import{_ as o,o as r,c as s,A as t}from"./entry.Cn1P5DRm.js";const c={};function n(e,a){return r(),s("em",null,[t(e.$slots,"default")])}const _=o(c,[["render",n]]);export{_ as default};
+import{_ as o,o as r,c as s,A as t}from"./entry.ByxzK2uc.js";const c={};function n(e,a){return r(),s("em",null,[t(e.$slots,"default")])}const _=o(c,[["render",n]]);export{_ as default};
diff --git a/.output/public/static/ProseH1.uaqQCKch.js b/.output/public/static/ProseH1.zvrFYIzS.js
similarity index 88%
rename from .output/public/static/ProseH1.uaqQCKch.js
rename to .output/public/static/ProseH1.zvrFYIzS.js
index 88378448d61d884f2bc40ed8703c178e0669b1da..6d01a9cce5bef79245540ab07327909687a916b5 100644
--- a/.output/public/static/ProseH1.uaqQCKch.js
+++ b/.output/public/static/ProseH1.zvrFYIzS.js
@@ -1 +1 @@
-import{y as p,aa as f,m as u,o as t,c as s,g as i,A as n}from"./entry.Cn1P5DRm.js";const l=["id"],m=["href"],_=p({__name:"ProseH1",props:{id:{}},setup(r){const c=r,{headings:o}=f().public.mdc,a=u(()=>{var e;return c.id&&(typeof(o==null?void 0:o.anchorLinks)=="boolean"&&(o==null?void 0:o.anchorLinks)===!0||typeof(o==null?void 0:o.anchorLinks)=="object"&&((e=o==null?void 0:o.anchorLinks)==null?void 0:e.h1))});return(e,d)=>(t(),s("h1",{id:e.id},[i(a)?(t(),s("a",{key:0,href:`#${e.id}`},[n(e.$slots,"default")],8,m)):n(e.$slots,"default",{key:1})],8,l))}});export{_ as default};
+import{y as p,aa as f,m as u,o as t,c as s,g as i,A as n}from"./entry.ByxzK2uc.js";const l=["id"],m=["href"],_=p({__name:"ProseH1",props:{id:{}},setup(r){const c=r,{headings:o}=f().public.mdc,a=u(()=>{var e;return c.id&&(typeof(o==null?void 0:o.anchorLinks)=="boolean"&&(o==null?void 0:o.anchorLinks)===!0||typeof(o==null?void 0:o.anchorLinks)=="object"&&((e=o==null?void 0:o.anchorLinks)==null?void 0:e.h1))});return(e,d)=>(t(),s("h1",{id:e.id},[i(a)?(t(),s("a",{key:0,href:`#${e.id}`},[n(e.$slots,"default")],8,m)):n(e.$slots,"default",{key:1})],8,l))}});export{_ as default};
diff --git a/.output/public/static/ProseH2.BAddKv8c.js b/.output/public/static/ProseH2.q4hsESC5.js
similarity index 89%
rename from .output/public/static/ProseH2.BAddKv8c.js
rename to .output/public/static/ProseH2.q4hsESC5.js
index d62d220f86d02d5e3e9d6ec519321ca359d15ebc..4d4961297f870e28e0637d2c054414375f21c0a8 100644
--- a/.output/public/static/ProseH2.BAddKv8c.js
+++ b/.output/public/static/ProseH2.q4hsESC5.js
@@ -1 +1 @@
-import{y as p,aa as f,m as i,o as t,c as s,g as u,A as n}from"./entry.Cn1P5DRm.js";const l=["id"],m=["href"],y=p({__name:"ProseH2",props:{id:{}},setup(r){const a=r,{headings:o}=f().public.mdc,c=i(()=>{var e;return a.id&&(typeof(o==null?void 0:o.anchorLinks)=="boolean"&&(o==null?void 0:o.anchorLinks)===!0||typeof(o==null?void 0:o.anchorLinks)=="object"&&((e=o==null?void 0:o.anchorLinks)==null?void 0:e.h2))});return(e,d)=>(t(),s("h2",{id:e.id},[e.id&&u(c)?(t(),s("a",{key:0,href:`#${e.id}`},[n(e.$slots,"default")],8,m)):n(e.$slots,"default",{key:1})],8,l))}});export{y as default};
+import{y as p,aa as f,m as i,o as t,c as s,g as u,A as n}from"./entry.ByxzK2uc.js";const l=["id"],m=["href"],y=p({__name:"ProseH2",props:{id:{}},setup(r){const a=r,{headings:o}=f().public.mdc,c=i(()=>{var e;return a.id&&(typeof(o==null?void 0:o.anchorLinks)=="boolean"&&(o==null?void 0:o.anchorLinks)===!0||typeof(o==null?void 0:o.anchorLinks)=="object"&&((e=o==null?void 0:o.anchorLinks)==null?void 0:e.h2))});return(e,d)=>(t(),s("h2",{id:e.id},[e.id&&u(c)?(t(),s("a",{key:0,href:`#${e.id}`},[n(e.$slots,"default")],8,m)):n(e.$slots,"default",{key:1})],8,l))}});export{y as default};
diff --git a/.output/public/static/ProseH3.C6POiIR1.js b/.output/public/static/ProseH3.BOc6mzCb.js
similarity index 89%
rename from .output/public/static/ProseH3.C6POiIR1.js
rename to .output/public/static/ProseH3.BOc6mzCb.js
index beef67d3b77c0542b98de6808afecf08bfbfff2b..157478c12b93e99a7430d212f5603ecff4f1dfee 100644
--- a/.output/public/static/ProseH3.C6POiIR1.js
+++ b/.output/public/static/ProseH3.BOc6mzCb.js
@@ -1 +1 @@
-import{y as p,aa as f,m as i,o as t,c as s,g as u,A as n}from"./entry.Cn1P5DRm.js";const l=["id"],m=["href"],y=p({__name:"ProseH3",props:{id:{}},setup(r){const a=r,{headings:o}=f().public.mdc,c=i(()=>{var e;return a.id&&(typeof(o==null?void 0:o.anchorLinks)=="boolean"&&(o==null?void 0:o.anchorLinks)===!0||typeof(o==null?void 0:o.anchorLinks)=="object"&&((e=o==null?void 0:o.anchorLinks)==null?void 0:e.h3))});return(e,d)=>(t(),s("h3",{id:e.id},[e.id&&u(c)?(t(),s("a",{key:0,href:`#${e.id}`},[n(e.$slots,"default")],8,m)):n(e.$slots,"default",{key:1})],8,l))}});export{y as default};
+import{y as p,aa as f,m as i,o as t,c as s,g as u,A as n}from"./entry.ByxzK2uc.js";const l=["id"],m=["href"],y=p({__name:"ProseH3",props:{id:{}},setup(r){const a=r,{headings:o}=f().public.mdc,c=i(()=>{var e;return a.id&&(typeof(o==null?void 0:o.anchorLinks)=="boolean"&&(o==null?void 0:o.anchorLinks)===!0||typeof(o==null?void 0:o.anchorLinks)=="object"&&((e=o==null?void 0:o.anchorLinks)==null?void 0:e.h3))});return(e,d)=>(t(),s("h3",{id:e.id},[e.id&&u(c)?(t(),s("a",{key:0,href:`#${e.id}`},[n(e.$slots,"default")],8,m)):n(e.$slots,"default",{key:1})],8,l))}});export{y as default};
diff --git a/.output/public/static/ProseH4.BhbnGrWY.js b/.output/public/static/ProseH4.D9WB5w3J.js
similarity index 89%
rename from .output/public/static/ProseH4.BhbnGrWY.js
rename to .output/public/static/ProseH4.D9WB5w3J.js
index 3006ce7dfdd5f70928189957bbf5a0df6340e642..6442d3c77fcfb3eba58c7d2e7ea09af99e5d8329 100644
--- a/.output/public/static/ProseH4.BhbnGrWY.js
+++ b/.output/public/static/ProseH4.D9WB5w3J.js
@@ -1 +1 @@
-import{y as p,aa as f,m as i,o as t,c as s,g as u,A as n}from"./entry.Cn1P5DRm.js";const l=["id"],m=["href"],y=p({__name:"ProseH4",props:{id:{}},setup(r){const a=r,{headings:o}=f().public.mdc,c=i(()=>{var e;return a.id&&(typeof(o==null?void 0:o.anchorLinks)=="boolean"&&(o==null?void 0:o.anchorLinks)===!0||typeof(o==null?void 0:o.anchorLinks)=="object"&&((e=o==null?void 0:o.anchorLinks)==null?void 0:e.h4))});return(e,d)=>(t(),s("h4",{id:e.id},[e.id&&u(c)?(t(),s("a",{key:0,href:`#${e.id}`},[n(e.$slots,"default")],8,m)):n(e.$slots,"default",{key:1})],8,l))}});export{y as default};
+import{y as p,aa as f,m as i,o as t,c as s,g as u,A as n}from"./entry.ByxzK2uc.js";const l=["id"],m=["href"],y=p({__name:"ProseH4",props:{id:{}},setup(r){const a=r,{headings:o}=f().public.mdc,c=i(()=>{var e;return a.id&&(typeof(o==null?void 0:o.anchorLinks)=="boolean"&&(o==null?void 0:o.anchorLinks)===!0||typeof(o==null?void 0:o.anchorLinks)=="object"&&((e=o==null?void 0:o.anchorLinks)==null?void 0:e.h4))});return(e,d)=>(t(),s("h4",{id:e.id},[e.id&&u(c)?(t(),s("a",{key:0,href:`#${e.id}`},[n(e.$slots,"default")],8,m)):n(e.$slots,"default",{key:1})],8,l))}});export{y as default};
diff --git a/.output/public/static/ProseH5.CcsuOqm6.js b/.output/public/static/ProseH5.DoxNaJQA.js
similarity index 89%
rename from .output/public/static/ProseH5.CcsuOqm6.js
rename to .output/public/static/ProseH5.DoxNaJQA.js
index a9d6d00cc9383089ce65679f8bd724ea3fcb96f4..ef6681818cb8055671f82e072d0df2a2adde8410 100644
--- a/.output/public/static/ProseH5.CcsuOqm6.js
+++ b/.output/public/static/ProseH5.DoxNaJQA.js
@@ -1 +1 @@
-import{y as p,aa as f,m as i,o as t,c as s,g as u,A as n}from"./entry.Cn1P5DRm.js";const l=["id"],m=["href"],y=p({__name:"ProseH5",props:{id:{}},setup(r){const a=r,{headings:o}=f().public.mdc,c=i(()=>{var e;return a.id&&(typeof(o==null?void 0:o.anchorLinks)=="boolean"&&(o==null?void 0:o.anchorLinks)===!0||typeof(o==null?void 0:o.anchorLinks)=="object"&&((e=o==null?void 0:o.anchorLinks)==null?void 0:e.h5))});return(e,d)=>(t(),s("h5",{id:e.id},[e.id&&u(c)?(t(),s("a",{key:0,href:`#${e.id}`},[n(e.$slots,"default")],8,m)):n(e.$slots,"default",{key:1})],8,l))}});export{y as default};
+import{y as p,aa as f,m as i,o as t,c as s,g as u,A as n}from"./entry.ByxzK2uc.js";const l=["id"],m=["href"],y=p({__name:"ProseH5",props:{id:{}},setup(r){const a=r,{headings:o}=f().public.mdc,c=i(()=>{var e;return a.id&&(typeof(o==null?void 0:o.anchorLinks)=="boolean"&&(o==null?void 0:o.anchorLinks)===!0||typeof(o==null?void 0:o.anchorLinks)=="object"&&((e=o==null?void 0:o.anchorLinks)==null?void 0:e.h5))});return(e,d)=>(t(),s("h5",{id:e.id},[e.id&&u(c)?(t(),s("a",{key:0,href:`#${e.id}`},[n(e.$slots,"default")],8,m)):n(e.$slots,"default",{key:1})],8,l))}});export{y as default};
diff --git a/.output/public/static/ProseH6.BIyhl79-.js b/.output/public/static/ProseH6.BTFBvSmu.js
similarity index 89%
rename from .output/public/static/ProseH6.BIyhl79-.js
rename to .output/public/static/ProseH6.BTFBvSmu.js
index 62d14b9ff67a3289d7968902aa84b831f5c3f77f..90651385384d1efa4c194f0d0773d18ab87955fd 100644
--- a/.output/public/static/ProseH6.BIyhl79-.js
+++ b/.output/public/static/ProseH6.BTFBvSmu.js
@@ -1 +1 @@
-import{y as p,aa as f,m as i,o as t,c as s,g as u,A as n}from"./entry.Cn1P5DRm.js";const l=["id"],m=["href"],y=p({__name:"ProseH6",props:{id:{}},setup(r){const a=r,{headings:o}=f().public.mdc,c=i(()=>{var e;return a.id&&(typeof(o==null?void 0:o.anchorLinks)=="boolean"&&(o==null?void 0:o.anchorLinks)===!0||typeof(o==null?void 0:o.anchorLinks)=="object"&&((e=o==null?void 0:o.anchorLinks)==null?void 0:e.h6))});return(e,d)=>(t(),s("h6",{id:e.id},[e.id&&u(c)?(t(),s("a",{key:0,href:`#${e.id}`},[n(e.$slots,"default")],8,m)):n(e.$slots,"default",{key:1})],8,l))}});export{y as default};
+import{y as p,aa as f,m as i,o as t,c as s,g as u,A as n}from"./entry.ByxzK2uc.js";const l=["id"],m=["href"],y=p({__name:"ProseH6",props:{id:{}},setup(r){const a=r,{headings:o}=f().public.mdc,c=i(()=>{var e;return a.id&&(typeof(o==null?void 0:o.anchorLinks)=="boolean"&&(o==null?void 0:o.anchorLinks)===!0||typeof(o==null?void 0:o.anchorLinks)=="object"&&((e=o==null?void 0:o.anchorLinks)==null?void 0:e.h6))});return(e,d)=>(t(),s("h6",{id:e.id},[e.id&&u(c)?(t(),s("a",{key:0,href:`#${e.id}`},[n(e.$slots,"default")],8,m)):n(e.$slots,"default",{key:1})],8,l))}});export{y as default};
diff --git a/.output/public/static/ProseHr.CbrQNIUe.js b/.output/public/static/ProseHr.CT-MOa0V.js
similarity index 55%
rename from .output/public/static/ProseHr.CbrQNIUe.js
rename to .output/public/static/ProseHr.CT-MOa0V.js
index d53734ba2906e6a4043993ebee6b7d2ba443c55d..e5125d6354e8e1b22744e7b43bd141c53fd22fd1 100644
--- a/.output/public/static/ProseHr.CbrQNIUe.js
+++ b/.output/public/static/ProseHr.CT-MOa0V.js
@@ -1 +1 @@
-import{_ as e,o as r,c}from"./entry.Cn1P5DRm.js";const o={};function t(n,s){return r(),c("hr")}const a=e(o,[["render",t]]);export{a as default};
+import{_ as e,o as r,c}from"./entry.ByxzK2uc.js";const o={};function t(n,s){return r(),c("hr")}const a=e(o,[["render",t]]);export{a as default};
diff --git a/.output/public/static/ProseImg.C5mZlKie.js b/.output/public/static/ProseImg.C8cBpZd8.js
similarity index 82%
rename from .output/public/static/ProseImg.C5mZlKie.js
rename to .output/public/static/ProseImg.C8cBpZd8.js
index 7fe75fda448d688d42f4ad50324f7d3dc50e86de..f24329564dc85664c2a2d555bef80ca2e5739f4a 100644
--- a/.output/public/static/ProseImg.C5mZlKie.js
+++ b/.output/public/static/ProseImg.C8cBpZd8.js
@@ -1 +1 @@
-import{_ as c}from"./NuxtImg.vue.CxZEoKQV.js";import{y as m,aa as i,m as h,ao as u,af as l,ap as d,o as f,s as g,g as r,bb as p}from"./entry.Cn1P5DRm.js";const w=m({__name:"ProseImg",props:{src:{type:String,default:""},alt:{type:String,default:""},width:{type:[String,Number],default:void 0},height:{type:[String,Number],default:void 0}},setup(e){const n=i().public.mdc.useNuxtImage?c:"img",t=e,o=h(()=>{var s;if((s=t.src)!=null&&s.startsWith("/")&&!t.src.startsWith("//")){const a=u(l(i().app.baseURL));if(a!=="/"&&!t.src.startsWith(a))return d(a,t.src)}return t.src});return(s,a)=>(f(),g(p(r(n)),{src:r(o),alt:e.alt,width:e.width,height:e.height},null,8,["src","alt","width","height"]))}});export{w as default};
+import{_ as c}from"./NuxtImg.vue.f86GhESM.js";import{y as m,aa as i,m as h,ao as u,af as l,ap as d,o as f,s as g,g as r,bb as p}from"./entry.ByxzK2uc.js";const w=m({__name:"ProseImg",props:{src:{type:String,default:""},alt:{type:String,default:""},width:{type:[String,Number],default:void 0},height:{type:[String,Number],default:void 0}},setup(e){const n=i().public.mdc.useNuxtImage?c:"img",t=e,o=h(()=>{var s;if((s=t.src)!=null&&s.startsWith("/")&&!t.src.startsWith("//")){const a=u(l(i().app.baseURL));if(a!=="/"&&!t.src.startsWith(a))return d(a,t.src)}return t.src});return(s,a)=>(f(),g(p(r(n)),{src:r(o),alt:e.alt,width:e.width,height:e.height},null,8,["src","alt","width","height"]))}});export{w as default};
diff --git a/.output/public/static/ProseLi.u1TOcRjB.js b/.output/public/static/ProseLi.CipiNB5C.js
similarity index 65%
rename from .output/public/static/ProseLi.u1TOcRjB.js
rename to .output/public/static/ProseLi.CipiNB5C.js
index 5e9c8a9c43e636f5d6f3bc522e4d329bdc84ddf3..dc1f6ae5d03a4cd0aba6f1c5de38f9cb5dcde728 100644
--- a/.output/public/static/ProseLi.u1TOcRjB.js
+++ b/.output/public/static/ProseLi.CipiNB5C.js
@@ -1 +1 @@
-import{_ as o,o as r,c as s,A as t}from"./entry.Cn1P5DRm.js";const c={};function n(e,a){return r(),s("li",null,[t(e.$slots,"default")])}const _=o(c,[["render",n]]);export{_ as default};
+import{_ as o,o as r,c as s,A as t}from"./entry.ByxzK2uc.js";const c={};function n(e,a){return r(),s("li",null,[t(e.$slots,"default")])}const _=o(c,[["render",n]]);export{_ as default};
diff --git a/.output/public/static/ProseOl.BvWr8QVU.js b/.output/public/static/ProseOl.Dalk-DhC.js
similarity index 65%
rename from .output/public/static/ProseOl.BvWr8QVU.js
rename to .output/public/static/ProseOl.Dalk-DhC.js
index 92e214c6167d10c9c71fcc3ceeb8690d5eaf401e..345d476257e4bc0a990bb7239046a874d638941d 100644
--- a/.output/public/static/ProseOl.BvWr8QVU.js
+++ b/.output/public/static/ProseOl.Dalk-DhC.js
@@ -1 +1 @@
-import{_ as o,o as r,c as s,A as t}from"./entry.Cn1P5DRm.js";const c={};function n(e,l){return r(),s("ol",null,[t(e.$slots,"default")])}const _=o(c,[["render",n]]);export{_ as default};
+import{_ as o,o as r,c as s,A as t}from"./entry.ByxzK2uc.js";const c={};function n(e,l){return r(),s("ol",null,[t(e.$slots,"default")])}const _=o(c,[["render",n]]);export{_ as default};
diff --git a/.output/public/static/ProseP.BgRgHCSi.js b/.output/public/static/ProseP.BMQnyrQ0.js
similarity index 65%
rename from .output/public/static/ProseP.BgRgHCSi.js
rename to .output/public/static/ProseP.BMQnyrQ0.js
index a153a381f6506849594ba171a5a34da70e7fb949..0b68a8c9950ea09f2f7d78abc88467615a6ad706 100644
--- a/.output/public/static/ProseP.BgRgHCSi.js
+++ b/.output/public/static/ProseP.BMQnyrQ0.js
@@ -1 +1 @@
-import{_ as o,o as r,c as s,A as t}from"./entry.Cn1P5DRm.js";const c={};function n(e,a){return r(),s("p",null,[t(e.$slots,"default")])}const _=o(c,[["render",n]]);export{_ as default};
+import{_ as o,o as r,c as s,A as t}from"./entry.ByxzK2uc.js";const c={};function n(e,a){return r(),s("p",null,[t(e.$slots,"default")])}const _=o(c,[["render",n]]);export{_ as default};
diff --git a/.output/public/static/ProsePre.B6iQQGWe.js b/.output/public/static/ProsePre.Ch3kertt.js
similarity index 74%
rename from .output/public/static/ProsePre.B6iQQGWe.js
rename to .output/public/static/ProsePre.Ch3kertt.js
index 2f320a65c92697fdc5c2cd4c65b8e65900809451..384d87ecf106854c2696bd46fa88186751ebb6c4 100644
--- a/.output/public/static/ProsePre.B6iQQGWe.js
+++ b/.output/public/static/ProsePre.Ch3kertt.js
@@ -1 +1 @@
-import{_ as l}from"./ProseCode.vue.BamOH8Eq.js";import{y as n,o as s,s as i,e as o,a as r,n as g,C as u,A as f}from"./entry.Cn1P5DRm.js";const h=n({__name:"ProsePre",props:{code:{type:String,default:""},language:{type:String,default:null},filename:{type:String,default:null},highlights:{type:Array,default:()=>[]},meta:{type:String,default:null},class:{type:String,default:null},style:{type:[String,Object],default:null}},setup(e){return(a,m)=>{const t=l;return s(),i(t,{code:e.code,language:e.language,filename:e.filename,highlights:e.highlights,meta:e.meta},{default:o(()=>[r("pre",{class:g(a.$props.class),style:u(e.style)},[f(a.$slots,"default")],6)]),_:3},8,["code","language","filename","highlights","meta"])}}});export{h as default};
+import{_ as l}from"./ProseCode.vue.6rThu75v.js";import{y as n,o as s,s as i,e as o,a as r,n as g,C as u,A as f}from"./entry.ByxzK2uc.js";const h=n({__name:"ProsePre",props:{code:{type:String,default:""},language:{type:String,default:null},filename:{type:String,default:null},highlights:{type:Array,default:()=>[]},meta:{type:String,default:null},class:{type:String,default:null},style:{type:[String,Object],default:null}},setup(e){return(a,m)=>{const t=l;return s(),i(t,{code:e.code,language:e.language,filename:e.filename,highlights:e.highlights,meta:e.meta},{default:o(()=>[r("pre",{class:g(a.$props.class),style:u(e.style)},[f(a.$slots,"default")],6)]),_:3},8,["code","language","filename","highlights","meta"])}}});export{h as default};
diff --git a/.output/public/static/ProseScript.I8x2nDFO.js b/.output/public/static/ProseScript.CepBX4em.js
similarity index 86%
rename from .output/public/static/ProseScript.I8x2nDFO.js
rename to .output/public/static/ProseScript.CepBX4em.js
index a9a3d38cad33657cfb9e9ae02cf09582719e12d0..634f013299d372d25faebabac5b43e12fec4640a 100644
--- a/.output/public/static/ProseScript.I8x2nDFO.js
+++ b/.output/public/static/ProseScript.CepBX4em.js
@@ -1 +1 @@
-import{y as r,g as n,o as s,c as a,x as e,a as o,q as i}from"./entry.Cn1P5DRm.js";const c={key:0},u=r({__name:"ProseScript",props:{src:{type:String,default:""}},setup(d){return(p,t)=>n(!1)?(s(),a("div",c,t[0]||(t[0]=[e(" Rendering the "),o("code",null,"script",-1),e(" element is dangerous and is disabled by default. Consider implementing your own "),o("code",null,"ProseScript",-1),e(" element to have control over script rendering. ")]))):i("",!0)}});export{u as default};
+import{y as r,g as n,o as s,c as a,x as e,a as o,q as i}from"./entry.ByxzK2uc.js";const c={key:0},u=r({__name:"ProseScript",props:{src:{type:String,default:""}},setup(d){return(p,t)=>n(!1)?(s(),a("div",c,t[0]||(t[0]=[e(" Rendering the "),o("code",null,"script",-1),e(" element is dangerous and is disabled by default. Consider implementing your own "),o("code",null,"ProseScript",-1),e(" element to have control over script rendering. ")]))):i("",!0)}});export{u as default};
diff --git a/.output/public/static/ProseStrong.CKz1sCKS.js b/.output/public/static/ProseStrong.ymVciFfk.js
similarity index 66%
rename from .output/public/static/ProseStrong.CKz1sCKS.js
rename to .output/public/static/ProseStrong.ymVciFfk.js
index 591430d404ceba8b00f9f93878bdd8d5b4239d9c..2b5392360fdd5da14b5b26c6949a9eb671bb1e80 100644
--- a/.output/public/static/ProseStrong.CKz1sCKS.js
+++ b/.output/public/static/ProseStrong.ymVciFfk.js
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+import{_ as o,o as r,c as t,A as n}from"./entry.ByxzK2uc.js";const s={};function c(e,a){return r(),t("strong",null,[n(e.$slots,"default")])}const _=o(s,[["render",c]]);export{_ as default};
diff --git a/.output/public/static/ProseTable.Bf0Bh7DN.js b/.output/public/static/ProseTable.fvZBrCls.js
similarity index 66%
rename from .output/public/static/ProseTable.Bf0Bh7DN.js
rename to .output/public/static/ProseTable.fvZBrCls.js
index 57757676dcaf22cddd715e45ea622c82b8f1851f..55514df827a70f3ad9c683bd66b30c7858115075 100644
--- a/.output/public/static/ProseTable.Bf0Bh7DN.js
+++ b/.output/public/static/ProseTable.fvZBrCls.js
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+import{_ as o,o as r,c as t,A as s}from"./entry.ByxzK2uc.js";const a={};function c(e,n){return r(),t("table",null,[s(e.$slots,"default")])}const _=o(a,[["render",c]]);export{_ as default};
diff --git a/.output/public/static/ProseTbody.XUkBRQ4L.js b/.output/public/static/ProseTbody.Y-ryT7it.js
similarity index 66%
rename from .output/public/static/ProseTbody.XUkBRQ4L.js
rename to .output/public/static/ProseTbody.Y-ryT7it.js
index 90a5c0964493a9246a83b81636722879b7058ee7..d47f424201643fc93f3fe15bd36c3c12a1a89d7f 100644
--- a/.output/public/static/ProseTbody.XUkBRQ4L.js
+++ b/.output/public/static/ProseTbody.Y-ryT7it.js
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+import{_ as o,o as r,c as t,A as s}from"./entry.ByxzK2uc.js";const c={};function n(e,a){return r(),t("tbody",null,[s(e.$slots,"default")])}const _=o(c,[["render",n]]);export{_ as default};
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similarity index 65%
rename from .output/public/static/ProseTd.CWQuzW2_.js
rename to .output/public/static/ProseTd.DRsruXNj.js
index f7bd1ddf0fd9ef9ea8be81a1090db1bdd6219967..3b87c084c6e7523e7f847082ef99cf979a9780ba 100644
--- a/.output/public/static/ProseTd.CWQuzW2_.js
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+import{_ as o,o as r,c as t,A as s}from"./entry.ByxzK2uc.js";const c={};function n(e,a){return r(),t("td",null,[s(e.$slots,"default")])}const _=o(c,[["render",n]]);export{_ as default};
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similarity index 65%
rename from .output/public/static/ProseTh.C0KVQbA1.js
rename to .output/public/static/ProseTh.Cg7RkNwl.js
index 3d1787f31ecc854688ce6b66088abc3f18019f8a..e1110b9f12c0917eda016f0e7d81aedb5027a929 100644
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+import{_ as o,o as r,c as t,A as s}from"./entry.ByxzK2uc.js";const c={};function n(e,a){return r(),t("th",null,[s(e.$slots,"default")])}const _=o(c,[["render",n]]);export{_ as default};
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similarity index 66%
rename from .output/public/static/ProseThead.x5vdkc7d.js
rename to .output/public/static/ProseThead.D5EWyO76.js
index 76c7c032f7b41d39c5a4a1b0b19803bde8821db1..7e0f41e5dfd7bd206fa91bd1b2546df8a927a8bb 100644
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+import{_ as o,o as r,c as t,A as s}from"./entry.ByxzK2uc.js";const a={};function c(e,n){return r(),t("thead",null,[s(e.$slots,"default")])}const _=o(a,[["render",c]]);export{_ as default};
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similarity index 64%
rename from .output/public/static/ProseTr.BRiw-NxU.js
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index 899b2ad5b8f15ffab9da9d83f2902ebfd2411a07..5e787b5ffe79c2387fe8b7c8bc36c95fa553ab86 100644
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similarity index 65%
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index 08f4552312e2d45e889adb0db74857f805902005..7546ccbbc66aa11f8a06fd9f2888eafc46b4dae4 100644
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+++ b/.output/public/static/ProseUl.UtV9B0Ju.js
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+import{_ as o,o as r,c as s,A as t}from"./entry.ByxzK2uc.js";const c={};function n(e,l){return r(),s("ul",null,[t(e.$slots,"default")])}const _=o(c,[["render",n]]);export{_ as default};
diff --git a/.output/public/static/anchorscroll.BUn64R6T.js b/.output/public/static/anchorscroll.V7tjJcvA.js
similarity index 96%
rename from .output/public/static/anchorscroll.BUn64R6T.js
rename to .output/public/static/anchorscroll.V7tjJcvA.js
index efba6714a708615dac98f4204ccdddde26ce8c54..9c1366f1f2ee977e129aa64caa5b6b45d3ad83d5 100644
--- a/.output/public/static/anchorscroll.BUn64R6T.js
+++ b/.output/public/static/anchorscroll.V7tjJcvA.js
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+import{m as n,ac as r,ad as f}from"./entry.ByxzK2uc.js";const v=(l={})=>{const T=n(()=>{var e,c;const o=r(l);return(o==null?void 0:o.surfaces)??((e=o==null?void 0:o.toAnchor)==null?void 0:e.surfaces)??r((c=f().$anchorScroll)==null?void 0:c.defaults.surfaces)??[]}),i=n(()=>{var e,c,t;const o=r(l);return(o==null?void 0:o.scrollOptions)??((e=o==null?void 0:o.toAnchor)==null?void 0:e.scrollOptions)??r((t=(c=f().$anchorScroll)==null?void 0:c.defaults)==null?void 0:t.toAnchor)}),S=n(()=>{var e,c;const o=r(l);return(o==null?void 0:o.surfaces)??((e=o==null?void 0:o.toTop)==null?void 0:e.surfaces)??r((c=f().$anchorScroll)==null?void 0:c.defaults.surfaces)??[]}),d=n(()=>{var e,c,t;const o=r(l);return(o==null?void 0:o.scrollOptions)??((e=o==null?void 0:o.toTop)==null?void 0:e.scrollOptions)??r((t=(c=f().$anchorScroll)==null?void 0:c.defaults)==null?void 0:t.toTop)});return{scrollToAnchor(o){const e=r(o);let c=null;if(typeof e=="string")c=document.getElementById(e.replace(/^#/,""));else if(e instanceof HTMLElement)c=e;else{const s=r(e.id),m=r(e.sr);if(s)c=document.getElementById(s.replace(/^#/,""));else if(m)try{c=document.querySelector(m)}catch(b){console.error("[useAnchorScroll]: While select element from document, next error occurred:",b)}else console.error("[useAnchorScroll]: Wrong object provided to scrollToAnchor composable:","either 'id' and 'sr' (selector) are not provided in object",e)}if(!c)return!1;const{top:t,left:u}=c.getBoundingClientRect(),{behavior:A,offsetLeft:a,offsetTop:h}=r(i)??{},y={behavior:A,...a!==void 0&&{left:u+a},...h!==void 0&&{top:t+h}};for(const s of r(T))s.scrollBy(y);return!0},scrollToTop(){const{behavior:o,offsetLeft:e,offsetTop:c}=r(d)??{},t={behavior:o,left:e,top:c};for(const u of r(S))u.scrollTo(t)}}};export{v as u};
diff --git a/.output/public/static/attributions.BmX6UhlG.js b/.output/public/static/attributions.CuPcG0mW.js
similarity index 86%
rename from .output/public/static/attributions.BmX6UhlG.js
rename to .output/public/static/attributions.CuPcG0mW.js
index 6d2ea1d90d80bc0da17484876bc193ceb61a7993..ab3884df2017b146f316684c67a69c403ca16465 100644
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+import{u as e,c as o,a,o as i}from"./entry.ByxzK2uc.js";const r={class:"attr m-5 p-8 rounded-lg"},d={__name:"attributions",setup(s){e({title:"BIT iGEM 2024 - Attributions",meta:[{name:"description",content:"Welcome to the BIT Wiki"}]});function t(){console.log("iframe loaded")}return(n,c)=>(i(),o("div",r,[a("iframe",{style:{width:"100%",height:"90vh"},id:"igem-attribution-form",src:"https://attributions.igem.org?team=bit&year=2024",onLoad:t},null,32)]))}};export{d as default};
diff --git a/.output/public/static/builds/latest.json b/.output/public/static/builds/latest.json
index 93edea00fedb56210a6969fd8c6c9e2d0a838b3e..8672a8e2cd30553bad15d756746d4300c31a8125 100644
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new file mode 100644
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deleted file mode 100644
index e5a1fbf267932a1067e45784765da865f08b0fa0..0000000000000000000000000000000000000000
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similarity index 99%
rename from .output/public/static/client-db.BgUG8l1f.js
rename to .output/public/static/client-db.BXhUscuN.js
index 9d5e7112501d1ab6a0a104472171a96ee3e218c4..5f828e41b66b128da97f918cc3bbdce495ba2fe0 100644
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similarity index 71%
rename from .output/public/static/contribution.B4fyGVhK.js
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index 592f31906245680b1e8db5277fdb079cef88046f..bfc6018cdaabe546af85df26b3922e22985709cb 100644
--- a/.output/public/static/contribution.B4fyGVhK.js
+++ b/.output/public/static/contribution.zedswWL_.js
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new file mode 100644
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+.el-menu{--el-menu-bg-color:rgba(0,0,0,.3);--tw-backdrop-blur:blur(16px);-webkit-backdrop-filter:var(--tw-backdrop-blur) var(--tw-backdrop-brightness) var(--tw-backdrop-contrast) var(--tw-backdrop-grayscale) var(--tw-backdrop-hue-rotate) var(--tw-backdrop-invert) var(--tw-backdrop-opacity) var(--tw-backdrop-saturate) var(--tw-backdrop-sepia);backdrop-filter:var(--tw-backdrop-blur) var(--tw-backdrop-brightness) var(--tw-backdrop-contrast) var(--tw-backdrop-grayscale) var(--tw-backdrop-hue-rotate) var(--tw-backdrop-invert) var(--tw-backdrop-opacity) var(--tw-backdrop-saturate) var(--tw-backdrop-sepia);--el-menu-border-color:transparent;--el-menu-text-color:#fff;--el-menu-hover-bg-color:hsla(0,0%,100%,.5)}.el-menu .el-menu-item:focus:not(.el-menu-item:hover){--el-menu-hover-bg-color:transparent;--el-menu-hover-text-color:#fff}.el-menu .el-sub-menu{height:100%;padding-left:2.5rem;padding-right:2.5rem;padding-top:1.25rem}.el-menu .el-sub-menu .el-sub-menu__title{border:none;padding:0}.el-menu .el-sub-menu .el-sub-menu__title:hover{--el-menu-bg-color:transparent;--el-menu-hover-bg-color:transparent}.el-menu .el-sub-menu.is-active .el-sub-menu__title{border-style:none;font-weight:700}.el-menu .el-sub-menu .el-icon{display:none}.el-menu .el-sub-menu.is-active{font-weight:700}.el-menu .el-sub-menu.is-active:after{border-bottom:2px solid var(--el-menu-active-color);bottom:1.5rem;content:"";display:block;position:relative;width:100%}.el-menu:not(.el-menu--popup) .el-menu-item{padding-top:1.25rem;--el-menu-base-level-padding:2.5rem}.el-menu:not(.el-menu--popup) .el-menu-item.is-active{border-style:none;font-weight:700}.el-menu:not(.el-menu--popup) .el-menu-item.is-active:after{border-bottom:2px solid var(--el-menu-active-color);content:"";display:block;margin-top:2rem;position:absolute;width:40%}.el-menu .el-avatar--icon{--el-avatar-icon-size:1.5rem;--el-avatar-bg-color:transparent;border:2px solid #fff}.el-menu--horizontal{--el-menu-horizontal-height:100px;align-items:stretch}.el-menu--horizontal>.el-menu-item{border:none}.el-menu--vertical{width:100%}.el-menu--vertical .el-menu-item,.el-menu--vertical .el-sub-menu__title{justify-content:center;padding-top:0!important}.el-menu--vertical .el-sub-menu.is-active:after{bottom:0;margin:0 auto;width:50%}.el-popper{--el-bg-color-overlay:rgba(0,0,0,.3);--tw-backdrop-blur:blur(16px);-webkit-backdrop-filter:var(--tw-backdrop-blur) var(--tw-backdrop-brightness) var(--tw-backdrop-contrast) var(--tw-backdrop-grayscale) var(--tw-backdrop-hue-rotate) var(--tw-backdrop-invert) var(--tw-backdrop-opacity) var(--tw-backdrop-saturate) var(--tw-backdrop-sepia);backdrop-filter:var(--tw-backdrop-blur) var(--tw-backdrop-brightness) var(--tw-backdrop-contrast) var(--tw-backdrop-grayscale) var(--tw-backdrop-hue-rotate) var(--tw-backdrop-invert) var(--tw-backdrop-opacity) var(--tw-backdrop-saturate) var(--tw-backdrop-sepia);--el-border-color-light:transparent;--el-menu-bg-color:transparent}body{background-image:url(https://static.igem.wiki/teams/5358/banner/bg.webp);background-repeat:repeat-y;background-size:cover;color:#fff;margin:0 auto;overflow-x:hidden}body,header{max-width:1920px}header{z-index:10}@media (min-width:1920px){header{justify-self:center;left:0;right:0}}@media (max-width:1920px){header{left:0}}.bg-circle-1{background:radial-gradient(174.6% 46.76% at 49.95818122671177% 50.029158628736816%,#3e22f2cc 0,#3113f203);filter:blur(200px);height:1285.25px;opacity:.6;right:29px;top:-474.06px;transform:rotate(38.39deg);width:788.66px;z-index:-1}.bg-circle-2{background:radial-gradient(81.41% 46.79% at 49.985575316522294% 50.01216625873434%,#cf2dc966 0,#3113f200);bottom:-126px;filter:blur(150px);height:467.79px;left:-118px;opacity:.9;transform:rotate(-7.21deg);width:842.33px;z-index:-1}
diff --git a/.output/public/static/default.Cnt67YMa.js b/.output/public/static/default.z0hlD7_I.js
similarity index 99%
rename from .output/public/static/default.Cnt67YMa.js
rename to .output/public/static/default.z0hlD7_I.js
index f90dfc844c20228ffcdaf03a4fca4f1b66db93a2..b755fe867f8822d613787ae7fdfb0b0e1cdc81ef 100644
--- a/.output/public/static/default.Cnt67YMa.js
+++ b/.output/public/static/default.z0hlD7_I.js
@@ -1 +1 @@
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s=je(),{indexPath:r,parentMenu:o}=lt(s,x(()=>e.index)),a=R("menu"),f=R("sub-menu"),i=xe("rootMenu");i||Me(Ne,"can not inject root menu");const l=xe(`subMenu:${o.value.uid}`);l||Me(Ne,"can not inject sub menu");const b=A({}),g=A({});let M;const C=A(!1),X=A(),ce=A(null),G=x(()=>q.value==="horizontal"&&V.value?"bottom-start":"right-start"),Z=x(()=>q.value==="horizontal"&&V.value||q.value==="vertical"&&!i.props.collapse?e.expandCloseIcon&&e.expandOpenIcon?z.value?e.expandOpenIcon:e.expandCloseIcon:vt:e.collapseCloseIcon&&e.collapseOpenIcon?z.value?e.collapseOpenIcon:e.collapseCloseIcon:mt),V=x(()=>l.level===0),oe=x(()=>{const v=e.teleported;return v===void 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w("div",{class:[a.m(q.value),a.m("popup-container"),ee.value],onMouseenter:U=>E(U,100),onMouseleave:()=>S(!0),onFocus:U=>E(U,100)},[w("ul",{class:[a.b(),a.m("popup"),a.m(`popup-${G.value}`)],style:ie.value},[(O=n.default)==null?void 0:O.call(n)])])},default:()=>w("div",{class:f.e("title"),onClick:m},y)}):w(le,{},[w("div",{class:f.e("title"),ref:X,onClick:m},y),w(fn,{},{default:()=>{var O;return ot(w("ul",{role:"menu",class:[a.b(),a.m("inline")],style:ie.value},[(O=n.default)==null?void 0:O.call(n)]),[[kt,z.value]])}})]);return w("li",{class:[f.b(),f.is("active",Q.value),f.is("opened",z.value),f.is("disabled",e.disabled)],role:"menuitem",ariaHaspopup:!0,ariaExpanded:z.value,onMouseenter:E,onMouseleave:()=>S(),onFocus:E},[I])}}});const xn=fe({mode:{type:String,values:["horizontal","vertical"],default:"vertical"},defaultActive:{type:String,default:""},defaultOpeneds:{type:re(Array),default:()=>Pt([])},uniqueOpened:Boolean,router:Boolean,menuTrigger:{type:String,values:["hover","click"],default:"hover"},collapse:Boolean,backgroundColor:String,textColor:String,activeTextColor:String,closeOnClickOutside:Boolean,collapseTransition:{type:Boolean,default:!0},ellipsis:{type:Boolean,default:!0},popperOffset:{type:Number,default:6},ellipsisIcon:{type:te,default:()=>bt},popperEffect:{type:re(String),default:"dark"},popperClass:String,showTimeout:{type:Number,default:300},hideTimeout:{type:Number,default:300}}),He=e=>Array.isArray(e)&&e.every(n=>K(n)),Mn={close:(e,n)=>K(e)&&He(n),open:(e,n)=>K(e)&&He(n),select:(e,n,t,s)=>K(e)&&He(n)&&_t(t)&&(s===void 0||s instanceof Promise)};var En=L({name:"ElMenu",props:xn,emits:Mn,setup(e,{emit:n,slots:t,expose:s}){const r=je(),o=r.appContext.config.globalProperties.$router,a=A(),f=R("menu"),i=R("sub-menu"),l=A(-1),b=A(e.defaultOpeneds&&!e.collapse?e.defaultOpeneds.slice(0):[]),g=A(e.defaultActive),M=A({}),C=A({}),X=x(()=>e.mode==="horizontal"||e.mode==="vertical"&&e.collapse),ce=()=>{const p=g.value&&M.value[g.value];if(!p||e.mode==="horizontal"||e.collapse)return;p.indexPath.forEach(m=>{const E=C.value[m];E&&G(m,E.indexPath)})},G=(p,h)=>{b.value.includes(p)||(e.uniqueOpened&&(b.value=b.value.filter(m=>h.includes(m))),b.value.push(p),n("open",p,h))},Z=p=>{const h=b.value.indexOf(p);h!==-1&&b.value.splice(h,1)},V=(p,h)=>{Z(p),n("close",p,h)},oe=({index:p,indexPath:h})=>{b.value.includes(p)?V(p,h):G(p,h)},Se=p=>{(e.mode==="horizontal"||e.collapse)&&(b.value=[]);const{index:h,indexPath:m}=p;if(!(Ue(h)||Ue(m)))if(e.router&&o){const E=p.route||h,S=o.push(E).then(v=>(v||(g.value=h),v));n("select",h,m,{index:h,indexPath:m,route:E},S)}else g.value=h,n("select",h,m,{index:h,indexPath:m})},Te=p=>{const h=M.value,m=h[p]||g.value&&h[g.value]||h[e.defaultActive];m?g.value=m.index:g.value=p},z=p=>{const h=getComputedStyle(p),m=Number.parseInt(h.marginLeft,10),E=Number.parseInt(h.marginRight,10);return p.offsetWidth+m+E||0},Q=()=>{var p,h;if(!a.value)return-1;const m=Array.from((h=(p=a.value)==null?void 0:p.childNodes)!=null?h:[]).filter(ue=>ue.nodeName!=="#comment"&&(ue.nodeName!=="#text"||ue.nodeValue)),E=64,S=getComputedStyle(a.value),v=Number.parseInt(S.paddingLeft,10),y=Number.parseInt(S.paddingRight,10),I=a.value.clientWidth-v-y;let O=0,U=0;return m.forEach((ue,pt)=>{O+=z(ue),O<=I-E&&(U=pt+1)}),U===m.length?-1:U},q=p=>C.value[p].indexPath,J=(p,h=33.34)=>{let m;return()=>{m&&clearTimeout(m),m=setTimeout(()=>{p()},h)}};let ie=!0;const pe=()=>{if(l.value===Q())return;const p=()=>{l.value=-1,At(()=>{l.value=Q()})};ie?p():J(p)(),ie=!1};ne(()=>e.defaultActive,p=>{M.value[p]||(g.value=""),Te(p)}),ne(()=>e.collapse,p=>{p&&(b.value=[])}),ne(M.value,ce);let ee;It(()=>{e.mode==="horizontal"&&e.ellipsis?ee=gt(a,pe).stop:ee==null||ee()});const he=A(!1);{const p=S=>{C.value[S.index]=S},h=S=>{delete C.value[S.index]};Oe("rootMenu",ze({props:e,openedMenus:b,items:M,subMenus:C,activeIndex:g,isMenuPopup:X,addMenuItem:S=>{M.value[S.index]=S},removeMenuItem:S=>{delete M.value[S.index]},addSubMenu:p,removeSubMenu:h,openMenu:G,closeMenu:V,handleMenuItemClick:Se,handleSubMenuClick:oe})),Oe(`subMenu:${r.uid}`,{addSubMenu:p,removeSubMenu:h,mouseInChild:he,level:0})}De(()=>{e.mode==="horizontal"&&new hn(r.vnode.el,f.namespace.value)}),s({open:h=>{const{indexPath:m}=C.value[h];m.forEach(E=>G(E,m))},close:Z,handleResize:pe});const we=dt(e,0);return()=>{var p,h;let m=(h=(p=t.default)==null?void 0:p.call(t))!=null?h:[];const E=[];if(e.mode==="horizontal"&&a.value){const y=ye(m),I=l.value===-1?y:y.slice(0,l.value),O=l.value===-1?[]:y.slice(l.value);O!=null&&O.length&&e.ellipsis&&(m=I,E.push(w(Fe,{index:"sub-menu-more",class:i.e("hide-arrow"),popperOffset:e.popperOffset},{title:()=>w(We,{class:i.e("icon-more")},{default:()=>w(e.ellipsisIcon)}),default:()=>O})))}const S=e.closeOnClickOutside?[[on,()=>{b.value.length&&(he.value||(b.value.forEach(y=>n("close",y,q(y))),b.value=[]))}]]:[],v=ot(w("ul",{key:String(e.collapse),role:"menubar",ref:a,style:we.value,class:{[f.b()]:!0,[f.m(e.mode)]:!0,[f.m("collapse")]:e.collapse}},[...m,...E]),S);return e.collapseTransition&&e.mode==="vertical"?w(bn,()=>v):v}}});const Sn=fe({index:{type:re([String,null]),default:null},route:{type:re([String,Object])},disabled:Boolean}),Tn={click:e=>K(e.index)&&Array.isArray(e.indexPath)},$e="ElMenuItem",wn=L({name:$e,components:{ElTooltip:nt},props:Sn,emits:Tn,setup(e,{emit:n}){const t=je(),s=xe("rootMenu"),r=R("menu"),o=R("menu-item");s||Me($e,"can not inject root 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diff --git a/.output/public/static/description.BGAD_OLR.js b/.output/public/static/description.CmC8kAfX.js
similarity index 77%
rename from .output/public/static/description.BGAD_OLR.js
rename to .output/public/static/description.CmC8kAfX.js
index 01adec08c739ca152de43843ac47e01101bbc1d3..321f96798faefd9d1925ebc35ebf451bcf2b99fd 100644
--- a/.output/public/static/description.BGAD_OLR.js
+++ b/.output/public/static/description.CmC8kAfX.js
@@ -1 +1 @@
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diff --git a/.output/public/static/education.AliSrJcd.js b/.output/public/static/education.KnC8vv46.js
similarity index 99%
rename from .output/public/static/education.AliSrJcd.js
rename to .output/public/static/education.KnC8vv46.js
index c1271402b09d6aff0c51e6e0804ce42de1d44a29..03db152c88f17c34ffe52131184e3cef99814390 100644
--- a/.output/public/static/education.AliSrJcd.js
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v=null,S=null;a.rewind&&(e.isBeginning?S=a.virtual&&a.virtual.enabled&&e.virtual?e.virtual.slides.length-1:e.slides.length-1:e.isEnd&&(v=0));const m=(c-u[g])/T,E=g<a.slidesPerGroupSkip-1?1:a.slidesPerGroup;if(p>a.longSwipesMs){if(!a.longSwipes){e.slideTo(e.activeIndex);return}e.swipeDirection==="next"&&(m>=a.longSwipesRatio?e.slideTo(a.rewind&&e.isEnd?v:g+E):e.slideTo(g)),e.swipeDirection==="prev"&&(m>1-a.longSwipesRatio?e.slideTo(g+E):S!==null&&m<0&&Math.abs(m)>a.longSwipesRatio?e.slideTo(S):e.slideTo(g))}else{if(!a.shortSwipes){e.slideTo(e.activeIndex);return}e.navigation&&(s.target===e.navigation.nextEl||s.target===e.navigation.prevEl)?s.target===e.navigation.nextEl?e.slideTo(g+E):e.slideTo(g):(e.swipeDirection==="next"&&e.slideTo(v!==null?v:g+E),e.swipeDirection==="prev"&&e.slideTo(S!==null?S:g))}}function Le(){const t=this,{params:e,el:i}=t;if(i&&i.offsetWidth===0)return;e.breakpoints&&t.setBreakpoint();const{allowSlideNext:s,allowSlidePrev:n,snapGrid:r}=t,a=t.virtual&&t.params.virtual.enabled;t.allowSlideNext=!0,t.allowSlidePrev=!0,t.updateSize(),t.updateSlides(),t.updateSlidesClasses();const o=a&&e.loop;(e.slidesPerView==="auto"||e.slidesPerView>1)&&t.isEnd&&!t.isBeginning&&!t.params.centeredSlides&&!o?t.slideTo(t.slides.length-1,0,!1,!0):t.params.loop&&!a?t.slideToLoop(t.realIndex,0,!1,!0):t.slideTo(t.activeIndex,0,!1,!0),t.autoplay&&t.autoplay.running&&t.autoplay.paused&&(clearTimeout(t.autoplay.resizeTimeout),t.autoplay.resizeTimeout=setTimeout(()=>{t.autoplay&&t.autoplay.running&&t.autoplay.paused&&t.autoplay.resume()},500)),t.allowSlidePrev=n,t.allowSlideNext=s,t.params.watchOverflow&&r!==t.snapGrid&&t.checkOverflow()}function di(t){const e=this;e.enabled&&(e.allowClick||(e.params.preventClicks&&t.preventDefault(),e.params.preventClicksPropagation&&e.animating&&(t.stopPropagation(),t.stopImmediatePropagation())))}function ui(){const t=this,{wrapperEl:e,rtlTranslate:i,enabled:s}=t;if(!s)return;t.previousTranslate=t.translate,t.isHorizontal()?t.translate=-e.scrollLeft:t.translate=-e.scrollTop,t.translate===0&&(t.translate=0),t.updateActiveIndex(),t.updateSlidesClasses();let n;const r=t.maxTranslate()-t.minTranslate();r===0?n=0:n=(t.translate-t.minTranslate())/r,n!==t.progress&&t.updateProgress(i?-t.translate:t.translate),t.emit("setTranslate",t.translate,!1)}function fi(t){const e=this;ne(e,t.target),!(e.params.cssMode||e.params.slidesPerView!=="auto"&&!e.params.autoHeight)&&e.update()}function ci(){const t=this;t.documentTouchHandlerProceeded||(t.documentTouchHandlerProceeded=!0,t.params.touchReleaseOnEdges&&(t.el.style.touchAction="auto"))}const Re=(t,e)=>{const 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u=0;i.isHorizontal()?o=s?-t:t:l=t,n.roundLengths&&(o=Math.floor(o),l=Math.floor(l)),i.previousTranslate=i.translate,i.translate=i.isHorizontal()?o:l,n.cssMode?r[i.isHorizontal()?"scrollLeft":"scrollTop"]=i.isHorizontal()?-o:-l:n.virtualTranslate||(i.isHorizontal()?o-=i.cssOverflowAdjustment():l-=i.cssOverflowAdjustment(),r.style.transform=`translate3d(${o}px, ${l}px, ${u}px)`);let d;const f=i.maxTranslate()-i.minTranslate();f===0?d=0:d=(t-i.minTranslate())/f,d!==a&&i.updateProgress(t),i.emit("setTranslate",i.translate,e)}function kt(){return-this.snapGrid[0]}function Dt(){return-this.snapGrid[this.snapGrid.length-1]}function Vt(t,e,i,s,n){t===void 0&&(t=0),e===void 0&&(e=this.params.speed),i===void 0&&(i=!0),s===void 0&&(s=!0);const r=this,{params:a,wrapperEl:o}=r;if(r.animating&&a.preventInteractionOnTransition)return!1;const l=r.minTranslate(),u=r.maxTranslate();let d;if(s&&t>l?d=l:s&&t<u?d=u:d=t,r.updateProgress(d),a.cssMode){const 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t=="string"&&(t=parseInt(t,10));const r=this;let a=t;a<0&&(a=0);const{params:o,snapGrid:l,slidesGrid:u,previousIndex:d,activeIndex:f,rtlTranslate:p,wrapperEl:c,enabled:h}=r;if(!h&&!s&&!n||r.destroyed||r.animating&&o.preventInteractionOnTransition)return!1;typeof e>"u"&&(e=r.params.speed);const g=Math.min(r.params.slidesPerGroupSkip,a);let T=g+Math.floor((a-g)/r.params.slidesPerGroup);T>=l.length&&(T=l.length-1);const v=-l[T];if(o.normalizeSlideIndex)for(let b=0;b<u.length;b+=1){const M=-Math.floor(v*100),B=Math.floor(u[b]*100),P=Math.floor(u[b+1]*100);typeof u[b+1]<"u"?M>=B&&M<P-(P-B)/2?a=b:M>=B&&M<P&&(a=b+1):M>=B&&(a=b)}if(r.initialized&&a!==f&&(!r.allowSlideNext&&(p?v>r.translate&&v>r.minTranslate():v<r.translate&&v<r.minTranslate())||!r.allowSlidePrev&&v>r.translate&&v>r.maxTranslate()&&(f||0)!==a))return!1;a!==(d||0)&&i&&r.emit("beforeSlideChangeStart"),r.updateProgress(v);let S;a>f?S="next":a<f?S="prev":S="reset";const m=r.virtual&&r.params.virtual.enabled;if(!(m&&n)&&(p&&-v===r.translate||!p&&v===r.translate))return r.updateActiveIndex(a),o.autoHeight&&r.updateAutoHeight(),r.updateSlidesClasses(),o.effect!=="slide"&&r.setTranslate(v),S!=="reset"&&(r.transitionStart(i,S),r.transitionEnd(i,S)),!1;if(o.cssMode){const b=r.isHorizontal(),M=p?v:-v;if(e===0)m&&(r.wrapperEl.style.scrollSnapType="none",r._immediateVirtual=!0),m&&!r._cssModeVirtualInitialSet&&r.params.initialSlide>0?(r._cssModeVirtualInitialSet=!0,requestAnimationFrame(()=>{c[b?"scrollLeft":"scrollTop"]=M})):c[b?"scrollLeft":"scrollTop"]=M,m&&requestAnimationFrame(()=>{r.wrapperEl.style.scrollSnapType="",r._immediateVirtual=!1});else{if(!r.support.smoothScroll)return Ve({swiper:r,targetPosition:M,side:b?"left":"top"}),!0;c.scrollTo({[b?"left":"top"]:M,behavior:"smooth"})}return!0}return 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diff --git a/.output/public/static/engineering.JPUWHJx8.js b/.output/public/static/engineering.C90YXWqp.js
similarity index 84%
rename from .output/public/static/engineering.JPUWHJx8.js
rename to .output/public/static/engineering.C90YXWqp.js
index b0c60e91f82430aa971f6ebf46d4e1410bf4acd9..abb34e55f4e8e446b4fead37540bf0f4098fb0aa 100644
--- a/.output/public/static/engineering.JPUWHJx8.js
+++ b/.output/public/static/engineering.C90YXWqp.js
@@ -1 +1 @@
-import{_ as b}from"./NuxtImg.vue.CxZEoKQV.js";import E from"./ContentRenderer.DLx9YsgF.js";import S from"./ContentDoc.mFdAzJVs.js";import{u as k,r as p,b as A,w as B,c as l,d as u,a as e,e as I,F as m,o as s,f as x,n as f,g as c,t as g,q as $}from"./entry.Cn1P5DRm.js";import{u as N}from"./anchorscroll.BUn64R6T.js";import"./ContentRendererMarkdown.vue.DihjwSrL.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.DMaui_6Y.js";import"./ContentQuery.D8uFFL4u.js";import"./query.DUXt7E8c.js";const j={class:"flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"},q={class:"md:sticky md:top-[120px] md:w-[200px] md:h-[200px] md:mr-10"},F=["onClick"],M=["onClick"],X={__name:"engineering",setup(O){k({title:"BIT iGEM 2024 - Engineering",meta:[{name:"description",content:"Welcome to the BIT Wiki"}]});const{scrollToAnchor:_,scrollToTop:V}=N({toTop:{scrollOptions:{behavior:"smooth",offsetTop:0}},toAnchor:{scrollOptions:{behavior:"smooth",offsetTop:-120}}}),i=p(""),d=p(""),h=p("");function T(){for(var a=document.querySelectorAll(".content h2"),o=a.length-1;o>=0;o--)if(i.value>=a[o].offsetTop-130){console.log(a[o].id),d.value=a[o].id;break}for(var t=document.querySelectorAll(".content h3"),o=t.length-1;o>=0;o--)if(i.value>=t[o].offsetTop-130){console.log(t[o].id),h.value=t[o].id;break}}function w(){i.value=document.documentElement.scrollTop||document.body.scrollTop}return A(()=>{window.addEventListener("scroll",w)}),B(i,()=>T()),(a,o)=>{const t=b,y=E,C=S;return s(),l(m,null,[u(t,{provider:"iGEM",src:"/banner/project.webp",class:"inline-block w-full mt-[-1rem]",alt:"cover"}),o[1]||(o[1]=e("div",{class:"page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg"},"Engineering",-1)),e("div",j,[u(C,{path:"/project-engineering"},{default:I(({doc:v})=>[e("aside",q,[o[0]||(o[0]=e("h1",{class:"mt-0"},"TOC",-1)),e("ul",null,[(s(!0),l(m,null,x(v.body.toc.links,n=>(s(),l("li",{key:n.id,class:f(["my-5",{"text-[#C0F748] font-bold":c(d)==n.id}])},[e("a",{onClick:r=>c(_)(n.id),class:"cursor-pointer"},g(n.text),9,F),e("ul",{class:f([{hidden:c(d)!=n.id},"pl-5 pt-2"])},[(s(!0),l(m,null,x(n.children,r=>(s(),l(m,{key:r.id},[r.depth==3?(s(),l("li",{key:0,class:f([{"text-[#fff] font-normal":c(h)!=r.id},"mb-2"])},[e("a",{onClick:D=>c(_)(r.id),class:"cursor-pointer"},g(r.text),9,M)],2)):$("",!0)],64))),128))],2)],2))),128))])]),u(y,{value:v,class:"content"},null,8,["value"])]),_:1})])],64)}}};export{X as default};
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diff --git a/.output/public/static/entry.Cn1P5DRm.js b/.output/public/static/entry.ByxzK2uc.js
similarity index 97%
rename from .output/public/static/entry.Cn1P5DRm.js
rename to .output/public/static/entry.ByxzK2uc.js
index 5de51d53b539199db990262e0dc3a4f4c948e6a6..7dfdf641951375cf5e3d9900384edd817029becd 100644
--- a/.output/public/static/entry.Cn1P5DRm.js
+++ b/.output/public/static/entry.ByxzK2uc.js
@@ -17,18 +17,18 @@
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similarity index 95%
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diff --git a/.output/public/static/experiment.5t6Tjj_S.js b/.output/public/static/experiment.BCd1fpWU.js
similarity index 82%
rename from .output/public/static/experiment.5t6Tjj_S.js
rename to .output/public/static/experiment.BCd1fpWU.js
index b68201698ef1f62de0f6611c202365a52d0dea12..6b34609a7309e6727fbd6d7efa2ddc80198e47f8 100644
--- a/.output/public/static/experiment.5t6Tjj_S.js
+++ b/.output/public/static/experiment.BCd1fpWU.js
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similarity index 80%
rename from .output/public/static/hardware.ki6bjMyd.js
rename to .output/public/static/hardware.CX4YEX7G.js
index a2acb1d0356d4f4c01bde3942a50f8989ef9a468..fbff94b7460833553d6ee78dbf7e03f49966bc71 100644
--- a/.output/public/static/hardware.ki6bjMyd.js
+++ b/.output/public/static/hardware.CX4YEX7G.js
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similarity index 82%
rename from .output/public/static/human-practices.D9bzgSRK.js
rename to .output/public/static/human-practices.ZNMJVabC.js
index 90d75417b4ff3d42d99647c40b72471d8d0d54a6..c9503998ef6c380af16098c4558a20ca878bd1c8 100644
--- a/.output/public/static/human-practices.D9bzgSRK.js
+++ b/.output/public/static/human-practices.ZNMJVabC.js
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diff --git a/.output/public/static/index.smMrUi_K.js b/.output/public/static/index.DzLNzWGb.js
similarity index 99%
rename from .output/public/static/index.smMrUi_K.js
rename to .output/public/static/index.DzLNzWGb.js
index 3602b29299a91466c2de6c048c443f4d2eb5c669..df0e2f89567935b3f5527616a603bb6e4156f6cf 100644
--- a/.output/public/static/index.smMrUi_K.js
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diff --git a/.output/public/static/index.QreSNRZw.js b/.output/public/static/index.sd8jDE3x.js
similarity index 97%
rename from .output/public/static/index.QreSNRZw.js
rename to .output/public/static/index.sd8jDE3x.js
index 53723fbeeea55de000ba68ce68c12c553edfd5a1..b66e8360a090176ccdc2e78e48e6cdaff0974d37 100644
--- a/.output/public/static/index.QreSNRZw.js
+++ b/.output/public/static/index.sd8jDE3x.js
@@ -1 +1 @@
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A series of strange and inexplicable phenomena began to appear all over the world. Many reports about animals and humans are worrying: abnormal behavior, decreased reproductive capacity, and death of young. </p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left"> A dangerous class of chemicals is harming environmental health - environmental endocrine disruptors: (refers to exogenous chemical substances released into the environment by human production and life that affect the normal hormone levels in the bodies of humans and animals.) </p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left">Many familiar pesticides and material additives belong to environmental endocrine disruptors, and in fact, there are even more endocrine disruptors lurking around us...</p></h2>',4)),e("div",y,[t(a,{provider:"iGEM",src:"/home/border.webp",class:"block px-10 sticky top-[20vh] z-[3]","data-aos":"fade-down","data-aos-duration":"400","data-aos-offset":"0"}),t(a,{provider:"iGEM",src:"/home/p2-cloud.webp",class:"block px-10 sticky top-[20vh]","data-aos":"fade-down","data-aos-duration":"100","data-aos-offset":"400"}),t(a,{provider:"iGEM",src:"/home/p2-bg.webp",class:"block px-10 sticky top-[20vh]","data-aos":"fade-up","data-aos-duration":"500","data-aos-offset":"300"}),t(a,{provider:"iGEM",src:"/home/p2-moon.webp",class:"block px-10 sticky top-[20vh]","data-aos":"fade-down","data-aos-duration":"200","data-aos-offset":"800"}),t(a,{provider:"iGEM",src:"/home/p2-death.webp",class:"block px-10 sticky top-[20vh]","data-aos":"fade-down","data-aos-duration":"200","data-aos-offset":"1000"}),t(a,{provider:"iGEM",src:"/home/p2-hand.webp",class:"block px-10 sticky top-[20vh]","data-aos":"fade-zoom-in","data-aos-duration":"200","data-aos-offset":"1200"}),t(a,{provider:"iGEM",src:"/home/p2-drug.webp",class:"block px-10 sticky top-[20vh]","data-aos":"fade-zoom-in","data-aos-duration":"200","data-aos-offset":"1400"})]),o[3]||(o[3]=i('<h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left text-3xl font-extrabold 2xl:text-5xl">And it is our task to find out these substances.</p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left">The important mechanism of action of environmental endocrine disruptors in living organisms is to bind to hormone receptors, thereby disrupting the regulation of hormones. 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+import{_ as d}from"./NuxtImg.vue.f86GhESM.js";import{_ as p}from"./nuxt-link.B-U_8utk.js";import{_ as f,o as r,c as n,a as e,d as t,v as i,e as h,x as m,u,F as b}from"./entry.ByxzK2uc.js";const v={},x={class:"scrolling-animation m-20"},w={class:"my-20 h-[1000vh]"},y={class:"my-20 h-[800vh]"},g={class:"my-20 h-[400vh]"},k={class:"text-right"},_={class:"mt-20 text-right font-bold text-2xl hover:border-emerald-200 border-2 inline-block rounded-xl p-2 hover:text-emerald-300"};function E(l,o){const a=d,s=p;return r(),n("div",x,[o[1]||(o[1]=e("h2",{"data-aos":"fade-up","data-aos-duration":"1000"},[e("p",null,'"There was a strange stillness. The birds, for example — where had they gone? Many people spoke of them, puzzled and disturbed. The feeding stations in the backyards were deserted. The few birds seen anywhere were moribund; they trembled violently and could not fly. 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A series of strange and inexplicable phenomena began to appear all over the world. Many reports about animals and humans are worrying: abnormal behavior, decreased reproductive capacity, and death of young. </p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left"> A dangerous class of chemicals is harming environmental health - environmental endocrine disruptors: (refers to exogenous chemical substances released into the environment by human production and life that affect the normal hormone levels in the bodies of humans and animals.) </p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left">Many familiar pesticides and material additives belong to environmental endocrine disruptors, and in fact, there are even more endocrine disruptors lurking around us...</p></h2>',4)),e("div",y,[t(a,{provider:"iGEM",src:"/home/border.webp",class:"block px-10 sticky top-[20vh] z-[3]","data-aos":"fade-down","data-aos-duration":"400","data-aos-offset":"0"}),t(a,{provider:"iGEM",src:"/home/p2-cloud.webp",class:"block px-10 sticky top-[20vh]","data-aos":"fade-down","data-aos-duration":"100","data-aos-offset":"400"}),t(a,{provider:"iGEM",src:"/home/p2-bg.webp",class:"block px-10 sticky top-[20vh]","data-aos":"fade-up","data-aos-duration":"500","data-aos-offset":"300"}),t(a,{provider:"iGEM",src:"/home/p2-moon.webp",class:"block px-10 sticky top-[20vh]","data-aos":"fade-down","data-aos-duration":"200","data-aos-offset":"800"}),t(a,{provider:"iGEM",src:"/home/p2-death.webp",class:"block px-10 sticky top-[20vh]","data-aos":"fade-down","data-aos-duration":"200","data-aos-offset":"1000"}),t(a,{provider:"iGEM",src:"/home/p2-hand.webp",class:"block px-10 sticky top-[20vh]","data-aos":"fade-zoom-in","data-aos-duration":"200","data-aos-offset":"1200"}),t(a,{provider:"iGEM",src:"/home/p2-drug.webp",class:"block px-10 sticky top-[20vh]","data-aos":"fade-zoom-in","data-aos-duration":"200","data-aos-offset":"1400"})]),o[3]||(o[3]=i('<h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left text-3xl font-extrabold 2xl:text-5xl">And it is our task to find out these substances.</p></h2><h2 data-aos="fade-up" data-aos-duration="1000" class="my-20"><p class="text-left">The important mechanism of action of environmental endocrine disruptors in living organisms is to bind to hormone receptors, thereby disrupting the regulation of hormones. 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diff --git a/.output/public/static/member.qnUWIOjk.js b/.output/public/static/member.Ci-NnrCc.js
similarity index 98%
rename from .output/public/static/member.qnUWIOjk.js
rename to .output/public/static/member.Ci-NnrCc.js
index ff942e816b0ad6332aa8a1aa2856e7e1e3538fca..6a0920a7ad0bd4da09fdcee988f105b5bb7ce76d 100644
--- a/.output/public/static/member.qnUWIOjk.js
+++ b/.output/public/static/member.Ci-NnrCc.js
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Biological Sciences",content:"This year, I led the strain construction, verification, and characterization of biological circuits in wet lab experiments. Our project aims to advance biotoxicology detection methods.",offset:[10,-3]},{name:"Li Guanlong",pos:"Leader",src:"/team1/leader-liguanlong.jpg",degree:"Senior",major:"Biomedical Engineering",content:"A doer with full of enthusiasm. A dreamer with full of passion.",offset:[-5,-3]},{name:"Wang Jianing",pos:"Leader",src:"/team1/leader-wangjianing.jpg",degree:"Senior",major:"Biomedical Engineering",content:"Always appreciate the past, cherish the present and be enthusiastic to the future.",offset:[-10,0]},{name:"Cai Tong",pos:"Student",src:"/team1/students-caitong.jpg",degree:"Junior",major:"Biotechnology - AI",content:"A spirited young girl, driven by perfection, infuses everyday life with idealism, turning reality’s challenges into a realm of possibilities. Her pursuit of purpose is a continuous journey of discovery.",offset:[10,-2]},{name:"Cui Yuan",pos:"Student",src:"/team1/students-cuiyuan.jpg",degree:"Junior",major:"Biomedical Engineering",content:"As a member of the biology group, I have learned a lot of skills and further explored the field of synthetic biology that I am very interested in. I hope we can have a good result."},{name:"Gao Peng",pos:"Student",src:"/team1/students-gaopeng.jpg",degree:"Senior",major:"Software Engineering",content:"Dive deep into the neuroscience and healthcare. TECH FOR GOOD!"},{name:"Huo Yongyu",pos:"Student",src:"/team1/students-huoyongyu.jpg",degree:"Senior",major:"Software Engineering",content:"A student who likes code, likes to explore and likes the unknown world.",offset:[-10,0]},{name:"Jin Chuan",pos:"Student",src:"/team1/students-jinchuan.jpg",degree:"Sophomore",major:"Optoelectronic Information and Intelligent Sensing",content:"An idealistic girl who wants to become a promoter of the intersection of medicine and engineering."},{name:"Li Sixuan",pos:"Student",src:"/team1/students-lisixuan.jpg",degree:"Sophomore",major:"Product Design",content:"A life enthusiast, a sports aficionado, and a visionary who seeks to elevate the world through the transformative power of art and design."},{name:"Li Tian",pos:"Student",src:"/team1/students-litian.jpg",degree:"Junior",major:"Biomedical Engineering",content:"Hello everyone, my name is Li Tian. Nice to meet you.",offset:[20,-3]},{name:"Li Xichang",pos:"Student",src:"/team1/students-lixichang.jpg",degree:"Junior",major:"Biology",content:"He’s driven to use advanced life science tech for global benefits, believing it can solve key issues like disease and environmental damage, creating a healthier, sustainable world."},{name:"Li Zhuohan",pos:"Student",src:"/team1/students-leezhuohai.jpg",degree:"Twelfth Grade",major:"Beijing 101 High School",content:"Biology is one of the few subjects I am passionate about and willing to devote into a great deal of thought and time.  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If you want to know me, please don't give up because of offline indifference",offset:[-10,0]},{name:"Wen Junjie",pos:"Student",src:"/team1/students-wenjunjie.jpg",degree:"Junior",major:"Biomedical Engineering",content:"Gains Dont Stop!"},{name:"Yao Yixuan",pos:"Student",src:"/team1/students-yaoyixuan.jpg",degree:"Twelfth Grade",major:"Affiliated High School of Renmin University",content:"I have an upbeat personality with a love for literature, music, and sports. In my free time, I compose music and excel in various ball games. Robotics, however, is my true passion, and I’ve won awards in several competitions."},{name:"Zhang Lingyue",pos:"Student",src:"/team1/students-zhanglingyue.jpg",degree:"Junior",major:"Mechanical Engineering",content:"As a creative hardware designer, I aim to bring our innovative biological ideas to life through practical hardware solutions. 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diff --git a/.output/public/static/model.CG79Dgst.js b/.output/public/static/model.CLg9pA01.js
similarity index 99%
rename from .output/public/static/model.CG79Dgst.js
rename to .output/public/static/model.CLg9pA01.js
index 9089ae78bec1314cd6c49d4a7fed86a3e1159fc1..e0c4991f2427776beda2d2cecc441283cef65c92 100644
--- a/.output/public/static/model.CG79Dgst.js
+++ b/.output/public/static/model.CLg9pA01.js
@@ -1,4 +1,4 @@
-import{_ as xg}from"./NuxtImg.vue.CxZEoKQV.js";import{G as Vf,r as At,H as yg,I as Mn,y as fr,J as Gf,K as kn,L as Nh,m as dn,b as Mg,w as ri,M as Ci,o as fn,c as Pn,n as bg,g as Ye,C as wg,N as iu,p as ru,O as Sg,P as Eg,Q as Tg,R as Ag,k as Oh,S as ia,U as kt,V as Cg,W as Rg,X as Pg,Y as Ig,Z as Fh,$ as Lg,a0 as Dg,a1 as Wf,F as Xf,a2 as Ug,a3 as Iu,A as Ng,a4 as Og,a5 as Fg,q as Yf,s as qf,a as Qi,a6 as Bg,d as Si,a7 as zg,a8 as kg,a9 as Hg,_ as Vg,u as Gg,e as Bh,aa as Wg,ab as Xg}from"./entry.Cn1P5DRm.js";/**
+import{_ as xg}from"./NuxtImg.vue.f86GhESM.js";import{G as Vf,r as At,H as yg,I as Mn,y as fr,J as Gf,K as kn,L as Nh,m as dn,b as Mg,w as ri,M as Ci,o as fn,c as Pn,n as bg,g as Ye,C as wg,N as iu,p as ru,O as Sg,P as Eg,Q as Tg,R as Ag,k as Oh,S as ia,U as kt,V as Cg,W as Rg,X as Pg,Y as Ig,Z as Fh,$ as Lg,a0 as Dg,a1 as Wf,F as Xf,a2 as Ug,a3 as Iu,A as Ng,a4 as Og,a5 as Fg,q as Yf,s as qf,a as Qi,a6 as Bg,d as Si,a7 as zg,a8 as kg,a9 as Hg,_ as Vg,u as Gg,e as Bh,aa as Wg,ab as Xg}from"./entry.ByxzK2uc.js";/**
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  * Copyright 2010-2024 Three.js Authors
  * SPDX-License-Identifier: MIT
diff --git a/.output/public/static/notebook.CKeUwtj_.js b/.output/public/static/notebook.DE_cVsbN.js
similarity index 81%
rename from .output/public/static/notebook.CKeUwtj_.js
rename to .output/public/static/notebook.DE_cVsbN.js
index 700e914a4d5b8724bbb5298c629d1c4a2068e4bd..46c8831a47736bc5ebfd3a1c21ee42a6c3c75afd 100644
--- a/.output/public/static/notebook.CKeUwtj_.js
+++ b/.output/public/static/notebook.DE_cVsbN.js
@@ -1 +1 @@
-import{_ as C}from"./NuxtImg.vue.CxZEoKQV.js";import g from"./ContentRenderer.DLx9YsgF.js";import S from"./ContentDoc.mFdAzJVs.js";import{_ as I,u as N,r as p,b as A,w as B,c as r,d as f,a as t,e as E,F as d,o as l,f as x,n as u,g as c,t as b,q as $}from"./entry.Cn1P5DRm.js";import{u as q}from"./anchorscroll.BUn64R6T.js";import"./ContentRendererMarkdown.vue.DihjwSrL.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.DMaui_6Y.js";import"./ContentQuery.D8uFFL4u.js";import"./query.DUXt7E8c.js";const F={class:"flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"},M={class:"md:sticky md:top-[120px] md:w-[200px] md:h-[450px] md:mr-10"},O=["onClick"],V=["onClick"],D={__name:"notebook",setup(G){N({title:"BIT iGEM 2024 - Notebook",meta:[{name:"description",content:"Welcome to the BIT Wiki"}]});const{scrollToAnchor:_,scrollToTop:L}=q({toTop:{scrollOptions:{behavior:"smooth",offsetTop:0}},toAnchor:{scrollOptions:{behavior:"smooth",offsetTop:-120}}}),i=p(""),m=p(""),h=p("");function w(){for(var a=document.querySelectorAll(".content h2"),o=a.length-1;o>=0;o--)if(i.value>=a[o].offsetTop-130){console.log(a[o].id),m.value=a[o].id;break}for(var e=document.querySelectorAll(".content h3"),o=e.length-1;o>=0;o--)if(i.value>=e[o].offsetTop-130){console.log(e[o].id),h.value=e[o].id;break}}function T(){i.value=document.documentElement.scrollTop||document.body.scrollTop}return A(()=>{window.addEventListener("scroll",T)}),B(i,()=>w()),(a,o)=>{const e=C,k=g,y=S;return l(),r(d,null,[f(e,{provider:"iGEM",src:"/banner/wetlab.webp",class:"inline-block w-full mt-[-1rem]",alt:"cover"}),o[1]||(o[1]=t("div",{class:"page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg"},"Notebook",-1)),t("div",F,[f(y,{path:"/wetlab-notebook"},{default:E(({doc:v})=>[t("aside",M,[o[0]||(o[0]=t("h1",{class:"mt-0"},"TOC",-1)),t("ul",null,[(l(!0),r(d,null,x(v.body.toc.links,n=>(l(),r("li",{key:n.id,class:u(["my-5",{"text-[#C0F748] font-bold":c(m)==n.id}])},[t("a",{onClick:s=>c(_)(n.id),class:"cursor-pointer"},b(n.text),9,O),t("ul",{class:u([{hidden:c(m)!=n.id},"pl-5 pt-2"])},[(l(!0),r(d,null,x(n.children,s=>(l(),r(d,{key:s.id},[s.depth==3?(l(),r("li",{key:0,class:u([{"text-[#fff] font-normal":c(h)!=s.id},"mb-2"])},[t("a",{onClick:W=>c(_)(s.id),class:"cursor-pointer"},b(s.text),9,V)],2)):$("",!0)],64))),128))],2)],2))),128))])]),f(k,{value:v,class:"content"},null,8,["value"])]),_:1})])],64)}}},Z=I(D,[["__scopeId","data-v-dabe85f1"]]);export{Z as default};
+import{_ as C}from"./NuxtImg.vue.f86GhESM.js";import g from"./ContentRenderer.CcHeFhnS.js";import S from"./ContentDoc.D4SfD6pm.js";import{_ as I,u as N,r as p,b as A,w as B,c as r,d as f,a as t,e as E,F as d,o as l,f as x,n as u,g as c,t as b,q as $}from"./entry.ByxzK2uc.js";import{u as q}from"./anchorscroll.V7tjJcvA.js";import"./ContentRendererMarkdown.vue.BNzii3wK.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.CFiP-sCj.js";import"./ContentQuery.BkEWwCWH.js";import"./query.HgboQeEc.js";const F={class:"flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"},M={class:"md:sticky md:top-[120px] md:w-[200px] md:h-[450px] md:mr-10"},O=["onClick"],V=["onClick"],D={__name:"notebook",setup(G){N({title:"BIT iGEM 2024 - Notebook",meta:[{name:"description",content:"Welcome to the BIT Wiki"}]});const{scrollToAnchor:_,scrollToTop:L}=q({toTop:{scrollOptions:{behavior:"smooth",offsetTop:0}},toAnchor:{scrollOptions:{behavior:"smooth",offsetTop:-120}}}),i=p(""),m=p(""),h=p("");function w(){for(var a=document.querySelectorAll(".content h2"),o=a.length-1;o>=0;o--)if(i.value>=a[o].offsetTop-130){console.log(a[o].id),m.value=a[o].id;break}for(var e=document.querySelectorAll(".content h3"),o=e.length-1;o>=0;o--)if(i.value>=e[o].offsetTop-130){console.log(e[o].id),h.value=e[o].id;break}}function T(){i.value=document.documentElement.scrollTop||document.body.scrollTop}return A(()=>{window.addEventListener("scroll",T)}),B(i,()=>w()),(a,o)=>{const e=C,k=g,y=S;return l(),r(d,null,[f(e,{provider:"iGEM",src:"/banner/wetlab.webp",class:"inline-block w-full mt-[-1rem]",alt:"cover"}),o[1]||(o[1]=t("div",{class:"page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg"},"Notebook",-1)),t("div",F,[f(y,{path:"/wetlab-notebook"},{default:E(({doc:v})=>[t("aside",M,[o[0]||(o[0]=t("h1",{class:"mt-0"},"TOC",-1)),t("ul",null,[(l(!0),r(d,null,x(v.body.toc.links,n=>(l(),r("li",{key:n.id,class:u(["my-5",{"text-[#C0F748] font-bold":c(m)==n.id}])},[t("a",{onClick:s=>c(_)(n.id),class:"cursor-pointer"},b(n.text),9,O),t("ul",{class:u([{hidden:c(m)!=n.id},"pl-5 pt-2"])},[(l(!0),r(d,null,x(n.children,s=>(l(),r(d,{key:s.id},[s.depth==3?(l(),r("li",{key:0,class:u([{"text-[#fff] font-normal":c(h)!=s.id},"mb-2"])},[t("a",{onClick:W=>c(_)(s.id),class:"cursor-pointer"},b(s.text),9,V)],2)):$("",!0)],64))),128))],2)],2))),128))])]),f(k,{value:v,class:"content"},null,8,["value"])]),_:1})])],64)}}},Z=I(D,[["__scopeId","data-v-dabe85f1"]]);export{Z as default};
diff --git a/.output/public/static/nuxt-link.CLgB-HXB.js b/.output/public/static/nuxt-link.B-U_8utk.js
similarity index 98%
rename from .output/public/static/nuxt-link.CLgB-HXB.js
rename to .output/public/static/nuxt-link.B-U_8utk.js
index a2be65a2b6bb4b0d0e6a99b25c1797710036d411..f90fb666f55d5d562018a15a83b2ea14f8d07eb8 100644
--- a/.output/public/static/nuxt-link.CLgB-HXB.js
+++ b/.output/public/static/nuxt-link.B-U_8utk.js
@@ -1 +1 @@
-import{aC as _,y as A,m as b,ax as S,r as x,b as L,aD as T,aE as B,j as E,aF as N,k as C,as as w,ap as U,ay as I,aG as j,aH as D,aa as F,ad as R,aI as O,af as V,aq as H}from"./entry.Cn1P5DRm.js";async function q(t,r=_()){const{path:s,matched:e}=r.resolve(t);if(!e.length||(r._routePreloaded||(r._routePreloaded=new Set),r._routePreloaded.has(s)))return;const l=r._preloadPromises=r._preloadPromises||[];if(l.length>4)return Promise.all(l).then(()=>q(t,r));r._routePreloaded.add(s);const i=e.map(c=>{var a;return(a=c.components)==null?void 0:a.default}).filter(c=>typeof c=="function");for(const c of i){const a=Promise.resolve(c()).catch(()=>{}).finally(()=>l.splice(l.indexOf(a)));l.push(a)}await Promise.all(l)}const z=(...t)=>t.find(r=>r!==void 0),M="noopener noreferrer";function $(t){const r=t.componentName||"NuxtLink",s=(e,l)=>{if(!e||t.trailingSlash!=="append"&&t.trailingSlash!=="remove")return e;if(typeof e=="string")return P(e,t.trailingSlash);const i="path"in e?e.path:l(e).path;return{...e,name:void 0,path:P(i,t.trailingSlash)}};return A({name:r,props:{to:{type:[String,Object],default:void 0,required:!1},href:{type:[String,Object],default:void 0,required:!1},target:{type:String,default:void 0,required:!1},rel:{type:String,default:void 0,required:!1},noRel:{type:Boolean,default:void 0,required:!1},prefetch:{type:Boolean,default:void 0,required:!1},noPrefetch:{type:Boolean,default:void 0,required:!1},activeClass:{type:String,default:void 0,required:!1},exactActiveClass:{type:String,default:void 0,required:!1},prefetchedClass:{type:String,default:void 0,required:!1},replace:{type:Boolean,default:void 0,required:!1},ariaCurrentValue:{type:String,default:void 0,required:!1},external:{type:Boolean,default:void 0,required:!1},custom:{type:Boolean,default:void 0,required:!1}},setup(e,{slots:l}){const i=_(),c=F(),a=b(()=>{const n=e.to||e.href||"";return s(n,i.resolve)}),d=b(()=>typeof a.value=="string"&&S(a.value,{acceptRelative:!0})),v=b(()=>e.external||e.target&&e.target!=="_self"?!0:typeof a.value=="object"?!1:a.value===""||d.value),y=x(!1),h=x(null),k=n=>{var f;h.value=e.custom?(f=n==null?void 0:n.$el)==null?void 0:f.nextElementSibling:n==null?void 0:n.$el};if(e.prefetch!==!1&&e.noPrefetch!==!0&&e.target!=="_blank"&&!Q()){const f=R();let m,o=null;L(()=>{const p=G();T(()=>{m=B(()=>{var g;(g=h==null?void 0:h.value)!=null&&g.tagName&&(o=p.observe(h.value,async()=>{o==null||o(),o=null;const u=typeof a.value=="string"?a.value:i.resolve(a.value).fullPath;await Promise.all([f.hooks.callHook("link:prefetch",u).catch(()=>{}),!v.value&&q(a.value,i).catch(()=>{})]),y.value=!0}))})})}),E(()=>{m&&N(m),o==null||o(),o=null})}return()=>{var p,g;if(!v.value){const u={ref:k,to:a.value,activeClass:e.activeClass||t.activeClass,exactActiveClass:e.exactActiveClass||t.exactActiveClass,replace:e.replace,ariaCurrentValue:e.ariaCurrentValue,custom:e.custom};return e.custom||(y.value&&(u.class=e.prefetchedClass||t.prefetchedClass),u.rel=e.rel),C(w("RouterLink"),u,l.default)}const n=typeof a.value=="object"?((p=i.resolve(a.value))==null?void 0:p.href)??null:a.value&&!e.external&&!d.value?s(U(c.app.baseURL,a.value),i.resolve):a.value||null,f=e.target||null,m=e.noRel?null:z(e.rel,t.externalRelAttribute,n?M:"")||null,o=()=>O(n,{replace:e.replace});return e.custom?l.default?l.default({href:n,navigate:o,get route(){if(!n)return;const u=I(n);return{path:u.pathname,fullPath:u.pathname,get query(){return j(u.search)},hash:u.hash,params:{},name:void 0,matched:[],redirectedFrom:void 0,meta:{},href:n}},rel:m,target:f,isExternal:v.value,isActive:!1,isExactActive:!1}):null:C("a",{ref:h,href:n,rel:m,target:f},(g=l.default)==null?void 0:g.call(l))}}})}const X=$(D);function P(t,r){const s=r==="append"?V:H;return S(t)&&!t.startsWith("http")?t:s(t,!0)}function G(){const t=R();if(t._observer)return t._observer;let r=null;const s=new Map,e=(i,c)=>(r||(r=new IntersectionObserver(a=>{for(const d of a){const v=s.get(d.target);(d.isIntersecting||d.intersectionRatio>0)&&v&&v()}})),s.set(i,c),r.observe(i),()=>{s.delete(i),r.unobserve(i),s.size===0&&(r.disconnect(),r=null)});return t._observer={observe:e}}function Q(){const t=navigator.connection;return!!(t&&(t.saveData||/2g/.test(t.effectiveType)))}export{X as _};
+import{aC as _,y as A,m as b,ax as S,r as x,b as L,aD as T,aE as B,j as E,aF as N,k as C,as as w,ap as U,ay as I,aG as j,aH as D,aa as F,ad as R,aI as O,af as V,aq as H}from"./entry.ByxzK2uc.js";async function q(t,r=_()){const{path:s,matched:e}=r.resolve(t);if(!e.length||(r._routePreloaded||(r._routePreloaded=new Set),r._routePreloaded.has(s)))return;const l=r._preloadPromises=r._preloadPromises||[];if(l.length>4)return Promise.all(l).then(()=>q(t,r));r._routePreloaded.add(s);const i=e.map(c=>{var a;return(a=c.components)==null?void 0:a.default}).filter(c=>typeof c=="function");for(const c of i){const a=Promise.resolve(c()).catch(()=>{}).finally(()=>l.splice(l.indexOf(a)));l.push(a)}await Promise.all(l)}const z=(...t)=>t.find(r=>r!==void 0),M="noopener noreferrer";function $(t){const r=t.componentName||"NuxtLink",s=(e,l)=>{if(!e||t.trailingSlash!=="append"&&t.trailingSlash!=="remove")return e;if(typeof e=="string")return P(e,t.trailingSlash);const i="path"in e?e.path:l(e).path;return{...e,name:void 0,path:P(i,t.trailingSlash)}};return A({name:r,props:{to:{type:[String,Object],default:void 0,required:!1},href:{type:[String,Object],default:void 0,required:!1},target:{type:String,default:void 0,required:!1},rel:{type:String,default:void 0,required:!1},noRel:{type:Boolean,default:void 0,required:!1},prefetch:{type:Boolean,default:void 0,required:!1},noPrefetch:{type:Boolean,default:void 0,required:!1},activeClass:{type:String,default:void 0,required:!1},exactActiveClass:{type:String,default:void 0,required:!1},prefetchedClass:{type:String,default:void 0,required:!1},replace:{type:Boolean,default:void 0,required:!1},ariaCurrentValue:{type:String,default:void 0,required:!1},external:{type:Boolean,default:void 0,required:!1},custom:{type:Boolean,default:void 0,required:!1}},setup(e,{slots:l}){const i=_(),c=F(),a=b(()=>{const n=e.to||e.href||"";return s(n,i.resolve)}),d=b(()=>typeof a.value=="string"&&S(a.value,{acceptRelative:!0})),v=b(()=>e.external||e.target&&e.target!=="_self"?!0:typeof a.value=="object"?!1:a.value===""||d.value),y=x(!1),h=x(null),k=n=>{var f;h.value=e.custom?(f=n==null?void 0:n.$el)==null?void 0:f.nextElementSibling:n==null?void 0:n.$el};if(e.prefetch!==!1&&e.noPrefetch!==!0&&e.target!=="_blank"&&!Q()){const f=R();let m,o=null;L(()=>{const p=G();T(()=>{m=B(()=>{var g;(g=h==null?void 0:h.value)!=null&&g.tagName&&(o=p.observe(h.value,async()=>{o==null||o(),o=null;const u=typeof a.value=="string"?a.value:i.resolve(a.value).fullPath;await Promise.all([f.hooks.callHook("link:prefetch",u).catch(()=>{}),!v.value&&q(a.value,i).catch(()=>{})]),y.value=!0}))})})}),E(()=>{m&&N(m),o==null||o(),o=null})}return()=>{var p,g;if(!v.value){const u={ref:k,to:a.value,activeClass:e.activeClass||t.activeClass,exactActiveClass:e.exactActiveClass||t.exactActiveClass,replace:e.replace,ariaCurrentValue:e.ariaCurrentValue,custom:e.custom};return e.custom||(y.value&&(u.class=e.prefetchedClass||t.prefetchedClass),u.rel=e.rel),C(w("RouterLink"),u,l.default)}const n=typeof a.value=="object"?((p=i.resolve(a.value))==null?void 0:p.href)??null:a.value&&!e.external&&!d.value?s(U(c.app.baseURL,a.value),i.resolve):a.value||null,f=e.target||null,m=e.noRel?null:z(e.rel,t.externalRelAttribute,n?M:"")||null,o=()=>O(n,{replace:e.replace});return e.custom?l.default?l.default({href:n,navigate:o,get route(){if(!n)return;const u=I(n);return{path:u.pathname,fullPath:u.pathname,get query(){return j(u.search)},hash:u.hash,params:{},name:void 0,matched:[],redirectedFrom:void 0,meta:{},href:n}},rel:m,target:f,isExternal:v.value,isActive:!1,isExactActive:!1}):null:C("a",{ref:h,href:n,rel:m,target:f},(g=l.default)==null?void 0:g.call(l))}}})}const X=$(D);function P(t,r){const s=r==="append"?V:H;return S(t)&&!t.startsWith("http")?t:s(t,!0)}function G(){const t=R();if(t._observer)return t._observer;let r=null;const s=new Map,e=(i,c)=>(r||(r=new IntersectionObserver(a=>{for(const d of a){const v=s.get(d.target);(d.isIntersecting||d.intersectionRatio>0)&&v&&v()}})),s.set(i,c),r.observe(i),()=>{s.delete(i),r.unobserve(i),s.size===0&&(r.disconnect(),r=null)});return t._observer={observe:e}}function Q(){const t=navigator.connection;return!!(t&&(t.saveData||/2g/.test(t.effectiveType)))}export{X as _};
diff --git a/.output/public/static/parts.CUfivJBH.js b/.output/public/static/parts.DweU74X9.js
similarity index 80%
rename from .output/public/static/parts.CUfivJBH.js
rename to .output/public/static/parts.DweU74X9.js
index c757beded6e2fc27c6ccfdf78d8e8aebd7d404e6..3b92e23c1b2c4927e462d7b10ec5ec9f6fed97e2 100644
--- a/.output/public/static/parts.CUfivJBH.js
+++ b/.output/public/static/parts.DweU74X9.js
@@ -1 +1 @@
-import{_ as b}from"./NuxtImg.vue.CxZEoKQV.js";import w from"./ContentRenderer.DLx9YsgF.js";import T from"./ContentDoc.mFdAzJVs.js";import{_ as y,u as C,r as i,b as g,w as k,c as n,d as l,a as t,e as B,F as p,o as a,f as E,n as I,g as d,t as S}from"./entry.Cn1P5DRm.js";import{u as A}from"./anchorscroll.BUn64R6T.js";import"./ContentRendererMarkdown.vue.DihjwSrL.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.DMaui_6Y.js";import"./ContentQuery.D8uFFL4u.js";import"./query.DUXt7E8c.js";const $={class:"flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"},F={class:"md:sticky md:top-[120px] md:w-[200px] md:h-[200px] md:mr-10"},M=["onClick"],N={__name:"parts",setup(O){C({title:"BIT iGEM 2024 - Parts",meta:[{name:"description",content:"Welcome to the BIT Wiki"}]});const{scrollToAnchor:u,scrollToTop:D}=A({toTop:{scrollOptions:{behavior:"smooth",offsetTop:0}},toAnchor:{scrollOptions:{behavior:"smooth",offsetTop:-120}}}),r=i(""),c=i("");function f(){for(var e=document.querySelectorAll(".content h2"),o=e.length-1;o>=0;o--)if(r.value>=e[o].offsetTop-130){c.value=e[o].id;break}}function _(){r.value=document.documentElement.scrollTop||document.body.scrollTop}return g(()=>{window.addEventListener("scroll",_)}),k(r,()=>f()),(e,o)=>{const x=b,v=w,h=T;return a(),n(p,null,[l(x,{provider:"iGEM",src:"/banner/wetlab.webp",class:"inline-block w-full mt-[-1rem]",alt:"cover"}),o[1]||(o[1]=t("div",{class:"page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg"},"Parts",-1)),t("div",$,[l(h,{path:"/wetlab-parts"},{default:B(({doc:m})=>[t("aside",F,[o[0]||(o[0]=t("h1",{class:"mt-0"},"TOC",-1)),t("ul",null,[(a(!0),n(p,null,E(m.body.toc.links,s=>(a(),n("li",{key:s.id,class:I(["my-5",{"text-[#C0F748] font-bold":d(c)==s.id}])},[t("a",{onClick:G=>d(u)(s.id),class:"cursor-pointer"},S(s.text),9,M)],2))),128))])]),l(v,{value:m,class:"content"},null,8,["value"])]),_:1})])],64)}}},Q=y(N,[["__scopeId","data-v-1b4324c9"]]);export{Q as default};
+import{_ as b}from"./NuxtImg.vue.f86GhESM.js";import w from"./ContentRenderer.CcHeFhnS.js";import T from"./ContentDoc.D4SfD6pm.js";import{_ as y,u as C,r as i,b as g,w as k,c as n,d as l,a as t,e as B,F as p,o as a,f as E,n as I,g as d,t as S}from"./entry.ByxzK2uc.js";import{u as A}from"./anchorscroll.V7tjJcvA.js";import"./ContentRendererMarkdown.vue.BNzii3wK.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.CFiP-sCj.js";import"./ContentQuery.BkEWwCWH.js";import"./query.HgboQeEc.js";const $={class:"flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"},F={class:"md:sticky md:top-[120px] md:w-[200px] md:h-[200px] md:mr-10"},M=["onClick"],N={__name:"parts",setup(O){C({title:"BIT iGEM 2024 - Parts",meta:[{name:"description",content:"Welcome to the BIT Wiki"}]});const{scrollToAnchor:u,scrollToTop:D}=A({toTop:{scrollOptions:{behavior:"smooth",offsetTop:0}},toAnchor:{scrollOptions:{behavior:"smooth",offsetTop:-120}}}),r=i(""),c=i("");function f(){for(var e=document.querySelectorAll(".content h2"),o=e.length-1;o>=0;o--)if(r.value>=e[o].offsetTop-130){c.value=e[o].id;break}}function _(){r.value=document.documentElement.scrollTop||document.body.scrollTop}return g(()=>{window.addEventListener("scroll",_)}),k(r,()=>f()),(e,o)=>{const x=b,v=w,h=T;return a(),n(p,null,[l(x,{provider:"iGEM",src:"/banner/wetlab.webp",class:"inline-block w-full mt-[-1rem]",alt:"cover"}),o[1]||(o[1]=t("div",{class:"page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg"},"Parts",-1)),t("div",$,[l(h,{path:"/wetlab-parts"},{default:B(({doc:m})=>[t("aside",F,[o[0]||(o[0]=t("h1",{class:"mt-0"},"TOC",-1)),t("ul",null,[(a(!0),n(p,null,E(m.body.toc.links,s=>(a(),n("li",{key:s.id,class:I(["my-5",{"text-[#C0F748] font-bold":d(c)==s.id}])},[t("a",{onClick:G=>d(u)(s.id),class:"cursor-pointer"},S(s.text),9,M)],2))),128))])]),l(v,{value:m,class:"content"},null,8,["value"])]),_:1})])],64)}}},Q=y(N,[["__scopeId","data-v-1b4324c9"]]);export{Q as default};
diff --git a/.output/public/static/preview.DMaui_6Y.js b/.output/public/static/preview.CFiP-sCj.js
similarity index 88%
rename from .output/public/static/preview.DMaui_6Y.js
rename to .output/public/static/preview.CFiP-sCj.js
index 1174c47ffa580485e05313f9cbab642a79fa8f76..e17901d310ff16b2661e840c4a487c2dedcdee98 100644
--- a/.output/public/static/preview.DMaui_6Y.js
+++ b/.output/public/static/preview.CFiP-sCj.js
@@ -1 +1 @@
-import{aw as r,ae as o}from"./entry.Cn1P5DRm.js";const v=()=>({isEnabled:()=>{const e=o().query;return Object.prototype.hasOwnProperty.call(e,"preview")&&!e.preview?!1:!!(e.preview||r("previewToken").value||sessionStorage.getItem("previewToken"))},getPreviewToken:()=>r("previewToken").value||sessionStorage.getItem("previewToken")||void 0,setPreviewToken:e=>{r("previewToken").value=e,o().query.preview=e||"",e?sessionStorage.setItem("previewToken",e):sessionStorage.removeItem("previewToken"),window.location.reload()}});export{v as u};
+import{aw as r,ae as o}from"./entry.ByxzK2uc.js";const v=()=>({isEnabled:()=>{const e=o().query;return Object.prototype.hasOwnProperty.call(e,"preview")&&!e.preview?!1:!!(e.preview||r("previewToken").value||sessionStorage.getItem("previewToken"))},getPreviewToken:()=>r("previewToken").value||sessionStorage.getItem("previewToken")||void 0,setPreviewToken:e=>{r("previewToken").value=e,o().query.preview=e||"",e?sessionStorage.setItem("previewToken",e):sessionStorage.removeItem("previewToken"),window.location.reload()}});export{v as u};
diff --git a/.output/public/static/query.DUXt7E8c.js b/.output/public/static/query.HgboQeEc.js
similarity index 95%
rename from .output/public/static/query.DUXt7E8c.js
rename to .output/public/static/query.HgboQeEc.js
index 475bec79590adc5b2b0ea828c219a453f254b4fd..155fa70c40dcabe83bc5a13b3a492249e64b6b59 100644
--- a/.output/public/static/query.DUXt7E8c.js
+++ b/.output/public/static/query.HgboQeEc.js
@@ -1,7 +1,7 @@
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as b,h as c,se as d,J as e,re as f,b as g,ne as h,ae as i,O as j,G as k,te as o,ce as q,V as s,oe as u,x as w};
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as b,h as c,se as d,J as e,re as f,b as g,ne as h,ae as i,O as j,G as k,te as o,ce as q,V as s,oe as u,x as w};
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+    __vite__mapDeps.viteFileDeps = ["./client-db.BXhUscuN.js","./entry.ByxzK2uc.js","./entry.DCK6esyZ.css","./index.C-v3KzvZ.js","./preview.CFiP-sCj.js"]
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diff --git a/.output/public/static/result.jFe2nWI0.js b/.output/public/static/result.CzO6ZXLT.js
similarity index 80%
rename from .output/public/static/result.jFe2nWI0.js
rename to .output/public/static/result.CzO6ZXLT.js
index a56a9672defa86936118bbecb8d8154ceb063481..e6fe3cc9daa8c622539428817dbce14756d99477 100644
--- a/.output/public/static/result.jFe2nWI0.js
+++ b/.output/public/static/result.CzO6ZXLT.js
@@ -1 +1 @@
-import{_ as b}from"./NuxtImg.vue.CxZEoKQV.js";import w from"./ContentRenderer.DLx9YsgF.js";import T from"./ContentDoc.mFdAzJVs.js";import{_ as y,u as C,r as i,b as g,w as k,c as n,d as r,a as t,e as B,F as p,o as a,f as E,n as I,g as d,t as S}from"./entry.Cn1P5DRm.js";import{u as A}from"./anchorscroll.BUn64R6T.js";import"./ContentRendererMarkdown.vue.DihjwSrL.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.DMaui_6Y.js";import"./ContentQuery.D8uFFL4u.js";import"./query.DUXt7E8c.js";const $={class:"flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"},F={class:"md:sticky md:top-[120px] md:w-[200px] md:h-[400px] md:mr-10"},M=["onClick"],N={__name:"result",setup(O){C({title:"BIT iGEM 2024 - Results",meta:[{name:"description",content:"Welcome to the BIT Wiki"}]});const{scrollToAnchor:u,scrollToTop:R}=A({toTop:{scrollOptions:{behavior:"smooth",offsetTop:0}},toAnchor:{scrollOptions:{behavior:"smooth",offsetTop:-120}}}),l=i(""),c=i("");function f(){for(var e=document.querySelectorAll(".content h2"),o=e.length-1;o>=0;o--)if(l.value>=e[o].offsetTop-130){c.value=e[o].id;break}}function _(){l.value=document.documentElement.scrollTop||document.body.scrollTop}return g(()=>{window.addEventListener("scroll",_)}),k(l,()=>f()),(e,o)=>{const x=b,v=w,h=T;return a(),n(p,null,[r(x,{provider:"iGEM",src:"/banner/wetlab.webp",class:"inline-block w-full mt-[-1rem]",alt:"cover"}),o[1]||(o[1]=t("div",{class:"page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg"},"Results",-1)),t("div",$,[r(h,{path:"/wetlab-results"},{default:B(({doc:m})=>[t("aside",F,[o[0]||(o[0]=t("h1",{class:"mt-0"},"TOC",-1)),t("ul",null,[(a(!0),n(p,null,E(m.body.toc.links,s=>(a(),n("li",{key:s.id,class:I(["my-5",{"text-[#C0F748] font-bold":d(c)==s.id}])},[t("a",{onClick:D=>d(u)(s.id),class:"cursor-pointer"},S(s.text),9,M)],2))),128))])]),r(v,{value:m,class:"content"},null,8,["value"])]),_:1})])],64)}}},Q=y(N,[["__scopeId","data-v-0a95154d"]]);export{Q as default};
+import{_ as b}from"./NuxtImg.vue.f86GhESM.js";import w from"./ContentRenderer.CcHeFhnS.js";import T from"./ContentDoc.D4SfD6pm.js";import{_ as y,u as C,r as i,b as g,w as k,c as n,d as r,a as t,e as B,F as p,o as a,f as E,n as I,g as d,t as S}from"./entry.ByxzK2uc.js";import{u as A}from"./anchorscroll.V7tjJcvA.js";import"./ContentRendererMarkdown.vue.BNzii3wK.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.CFiP-sCj.js";import"./ContentQuery.BkEWwCWH.js";import"./query.HgboQeEc.js";const $={class:"flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"},F={class:"md:sticky md:top-[120px] md:w-[200px] md:h-[400px] md:mr-10"},M=["onClick"],N={__name:"result",setup(O){C({title:"BIT iGEM 2024 - Results",meta:[{name:"description",content:"Welcome to the BIT Wiki"}]});const{scrollToAnchor:u,scrollToTop:R}=A({toTop:{scrollOptions:{behavior:"smooth",offsetTop:0}},toAnchor:{scrollOptions:{behavior:"smooth",offsetTop:-120}}}),l=i(""),c=i("");function f(){for(var e=document.querySelectorAll(".content h2"),o=e.length-1;o>=0;o--)if(l.value>=e[o].offsetTop-130){c.value=e[o].id;break}}function _(){l.value=document.documentElement.scrollTop||document.body.scrollTop}return g(()=>{window.addEventListener("scroll",_)}),k(l,()=>f()),(e,o)=>{const x=b,v=w,h=T;return a(),n(p,null,[r(x,{provider:"iGEM",src:"/banner/wetlab.webp",class:"inline-block w-full mt-[-1rem]",alt:"cover"}),o[1]||(o[1]=t("div",{class:"page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg"},"Results",-1)),t("div",$,[r(h,{path:"/wetlab-results"},{default:B(({doc:m})=>[t("aside",F,[o[0]||(o[0]=t("h1",{class:"mt-0"},"TOC",-1)),t("ul",null,[(a(!0),n(p,null,E(m.body.toc.links,s=>(a(),n("li",{key:s.id,class:I(["my-5",{"text-[#C0F748] font-bold":d(c)==s.id}])},[t("a",{onClick:D=>d(u)(s.id),class:"cursor-pointer"},S(s.text),9,M)],2))),128))])]),r(v,{value:m,class:"content"},null,8,["value"])]),_:1})])],64)}}},Q=y(N,[["__scopeId","data-v-0a95154d"]]);export{Q as default};
diff --git a/.output/public/static/safety.GVCLZ269.js b/.output/public/static/safety.DHiad1Ci.js
similarity index 76%
rename from .output/public/static/safety.GVCLZ269.js
rename to .output/public/static/safety.DHiad1Ci.js
index 09ad2b5c39b66336e260849b3d24740e1ef292ba..f3788fa2b7a0b7acbd84680510c7a3f735bd6bde 100644
--- a/.output/public/static/safety.GVCLZ269.js
+++ b/.output/public/static/safety.DHiad1Ci.js
@@ -1 +1 @@
-import{_ as y}from"./NuxtImg.vue.CxZEoKQV.js";import T from"./ContentRenderer.DLx9YsgF.js";import b from"./ContentDoc.mFdAzJVs.js";import{u as w,r as i,b as C,w as g,c as r,d as l,a as t,e as k,F as p,o as a,f as S,n as B,g as d,t as E}from"./entry.Cn1P5DRm.js";import{u as A}from"./anchorscroll.BUn64R6T.js";import"./ContentRendererMarkdown.vue.DihjwSrL.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.DMaui_6Y.js";import"./ContentQuery.D8uFFL4u.js";import"./query.DUXt7E8c.js";const I={class:"flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"},$={class:"md:sticky md:top-[120px] md:w-[200px] md:h-[300px] md:mr-10"},j=["onClick"],K={__name:"safety",setup(F){w({title:"BIT iGEM 2024 - Safety",meta:[{name:"description",content:"Welcome to the BIT Wiki"}]});const{scrollToAnchor:f,scrollToTop:M}=A({toTop:{scrollOptions:{behavior:"smooth",offsetTop:0}},toAnchor:{scrollOptions:{behavior:"smooth",offsetTop:-120}}}),n=i(""),c=i("");function u(){for(var e=document.querySelectorAll(".content h2"),o=e.length-1;o>=0;o--)if(n.value>=e[o].offsetTop-130){c.value=e[o].id;break}}function _(){n.value=document.documentElement.scrollTop||document.body.scrollTop}return C(()=>{window.addEventListener("scroll",_)}),g(n,()=>u()),(e,o)=>{const x=y,h=T,v=b;return a(),r(p,null,[l(x,{provider:"iGEM",src:"/banner/project.webp",class:"inline-block w-full mt-[-1rem]",alt:"cover"}),o[1]||(o[1]=t("div",{class:"page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg"},"Safety",-1)),t("div",I,[l(v,{path:"/project-safety"},{default:k(({doc:m})=>[t("aside",$,[o[0]||(o[0]=t("h1",{class:"mt-0"},"TOC",-1)),t("ul",null,[(a(!0),r(p,null,S(m.body.toc.links,s=>(a(),r("li",{key:s.id,class:B(["my-5",{"text-[#C0F748] font-bold":d(c)==s.id}])},[t("a",{onClick:N=>d(f)(s.id),class:"cursor-pointer"},E(s.text),9,j)],2))),128))])]),l(h,{value:m,class:"content"},null,8,["value"])]),_:1})])],64)}}};export{K as default};
+import{_ as y}from"./NuxtImg.vue.f86GhESM.js";import T from"./ContentRenderer.CcHeFhnS.js";import b from"./ContentDoc.D4SfD6pm.js";import{u as w,r as i,b as C,w as g,c as r,d as l,a as t,e as k,F as p,o as a,f as S,n as B,g as d,t as E}from"./entry.ByxzK2uc.js";import{u as A}from"./anchorscroll.V7tjJcvA.js";import"./ContentRendererMarkdown.vue.BNzii3wK.js";import"./index.C-v3KzvZ.js";import"./node.Dnd51l0P.js";import"./preview.CFiP-sCj.js";import"./ContentQuery.BkEWwCWH.js";import"./query.HgboQeEc.js";const I={class:"flex flex-row flex-wrap md:flex-nowrap justify-center mx-10"},$={class:"md:sticky md:top-[120px] md:w-[200px] md:h-[300px] md:mr-10"},j=["onClick"],K={__name:"safety",setup(F){w({title:"BIT iGEM 2024 - Safety",meta:[{name:"description",content:"Welcome to the BIT Wiki"}]});const{scrollToAnchor:f,scrollToTop:M}=A({toTop:{scrollOptions:{behavior:"smooth",offsetTop:0}},toAnchor:{scrollOptions:{behavior:"smooth",offsetTop:-120}}}),n=i(""),c=i("");function u(){for(var e=document.querySelectorAll(".content h2"),o=e.length-1;o>=0;o--)if(n.value>=e[o].offsetTop-130){c.value=e[o].id;break}}function _(){n.value=document.documentElement.scrollTop||document.body.scrollTop}return C(()=>{window.addEventListener("scroll",_)}),g(n,()=>u()),(e,o)=>{const x=y,h=T,v=b;return a(),r(p,null,[l(x,{provider:"iGEM",src:"/banner/project.webp",class:"inline-block w-full mt-[-1rem]",alt:"cover"}),o[1]||(o[1]=t("div",{class:"page-title m-10 mb-16 font-normal text-3xl p-5 pl-8 rounded-lg"},"Safety",-1)),t("div",I,[l(v,{path:"/project-safety"},{default:k(({doc:m})=>[t("aside",$,[o[0]||(o[0]=t("h1",{class:"mt-0"},"TOC",-1)),t("ul",null,[(a(!0),r(p,null,S(m.body.toc.links,s=>(a(),r("li",{key:s.id,class:B(["my-5",{"text-[#C0F748] font-bold":d(c)==s.id}])},[t("a",{onClick:N=>d(f)(s.id),class:"cursor-pointer"},E(s.text),9,j)],2))),128))])]),l(h,{value:m,class:"content"},null,8,["value"])]),_:1})])],64)}}};export{K as default};
diff --git a/assets/css/content.scss b/assets/css/content.scss
index 6108bd1cac350142d49db20bd20575369f8e165e..428a25d43a77e554251bbf5f5a66231f359a054c 100644
--- a/assets/css/content.scss
+++ b/assets/css/content.scss
@@ -87,9 +87,25 @@
     .table-fig {
         @apply my-10;
 
+        tbody {
+            @apply leading-normal;
+        }
+
         td {
             @apply pt-5;
         }
+
+        th {
+            @apply border-none p-0;
+        }
+
+        tr:nth-child(odd) {
+            @apply bg-transparent;
+        }
+
+        img {
+            @apply m-2;
+        }
     }
 
     .paren-ol {
@@ -105,6 +121,30 @@
         }
     }
 
+
+    table {
+        @apply mx-auto;
+
+        thead {
+            @apply text-center;
+        }
+        th {
+            @apply border-y px-5 text-wrap break-keep whitespace-pre-wrap;
+            border-color: rgba(200, 213, 279, 1.0);
+        }
+        td {
+            // @apply border-y border-white;
+        }
+
+        tbody {
+            @apply text-center leading-10;
+
+            tr:nth-child(odd) {
+                background-color: rgba(218, 227, 245, 0.4);
+            }
+        }
+    }
+
     .reference {
         position: relative;
         padding-left: 2.5rem;
diff --git a/components/Header.vue b/components/Header.vue
index ecf59211a029745f547532d2c2659496c3fdd5ae..29413f3b783dc99aef7b9a675c43913f52a7e532 100644
--- a/components/Header.vue
+++ b/components/Header.vue
@@ -137,6 +137,11 @@ watch(
         .el-sub-menu__title {
             padding: 0;
             border: none;
+
+            &:hover {
+                --el-menu-bg-color: transparent;
+                --el-menu-hover-bg-color: transparent;
+            }
         }
 
         &.is-active .el-sub-menu__title {
diff --git a/content/drylab-hardware.md b/content/drylab-hardware.md
index 45590d1edb4458f353f9278a29149a67e4fc1ddd..88d44980153c7274e8f7caf0f54728994d7901ee 100644
--- a/content/drylab-hardware.md
+++ b/content/drylab-hardware.md
@@ -8,13 +8,10 @@ As EDCs continue to threaten aquatic environments and the quality of drinking wa
 
 To ensure that the hardware device truly meets the needs of system construction, we have adopted a typical engineering and project design approach, namely: **demand analysis, scheme proposal, feasibility verification, feedback , system improvement, and goal achievement.** This process is an iterative one, and after several cycles, it ultimately meets all the needs and objectives.
 
-
-
 ![](https://static.igem.wiki/teams/5358/hardware/1.png)
 
 [Figure 1 Hardware Design Flowchart]{.fig-title}
 
- 
 
 ## Demand Analysis
 
@@ -26,9 +23,6 @@ Since the concentration of **EDCs** in the detection samples is very low，which
 
 In order to enable smartphone software to automate the control of hardware devices and visualize the detection results, the construction of hardware devices needs to consider the transmission of data between software and hardware.
 
- 
-
-
 
 ## Scheme Proposal
 
@@ -36,31 +30,22 @@ In order to enable smartphone software to automate the control of hardware devic
 
 [Figure 2 First Edition Design Scheme]{.fig-title}
 
- 
 
 ![](https://static.igem.wiki/teams/5358/hardware/3.png)
 
 [Figure 3 Circuit Diagram]{.fig-title}
 
- 
 
 In the construction of the **temperature control module**, we employed an NTC thermistor as the **temperature sensor**, utilizing heating FILM and fan as the heat and cold sources respectively. To achieve **PID regulation** for **temperature sensing and control**, we used the PWM wave output from the **TB6612FNG motor drive** to adjust the power of the fan and heating FILM, thereby maintaining dynamic temperature stability.
 
 For the selection of the detection scheme, we comprehensively considered the detection accuracy and the portability of the device, ultimately choosing the **fluorescence detection module**. We initially selected the AS7341 as **fluorescence sensor** [1]. Concurrently, to ensure consistent fluorescence generation, we redesigned and optimized the optical path.
 
- After the marked fluorescence is excited by a blue LED, the fluorescence sensor collect and record real-time fluorescence signals, which can be visualized on the mobile software via a **communication module**.
+After the marked fluorescence is excited by a blue LED, the fluorescence sensor collect and record real-time fluorescence signals, which can be visualized on the mobile software via a **communication module**.
 
 For the communication module, we used the HC-05 Bluetooth module to connect the hardware system with the mobile software, enabling real-time monitoring of the hardware device's operating status and feedback of the detection results.
 
 In the initial version of the scheme, we used an STM32 chip as the **microcontroller Unit(MCU)**, connecting temperature control module, fluorescence detection module, and Bluetooth communication module, ensuring the normal operation of the device through programming instructions.
 
- 
-<!-- [
-    ![](https://static.igem.wiki/teams/5358/hardware/4.png)
-    [Figure 5:  First Edition Implementation Scheme]{.block .fig-title}
-    ![](https://static.igem.wiki/teams/5358/hardware/5.png)
-    [Figure 6: First Edition Equipment Model Diagram]{.block .fig-title}
-]{.double-fig} -->
 
 ::mdc-wrap{:class="table-fig"}
 
@@ -70,8 +55,6 @@ In the initial version of the scheme, we used an STM32 chip as the **microcontro
 
 ::
 
- 
-
 
 
 ### Feasibility Verification
@@ -81,7 +64,6 @@ In the initial version of the scheme, we used an STM32 chip as the **microcontro
 To verify the constant temperature effect of the temperature control system, we designed a temperature stability test experiment. When the temperature first reached 37°C, the temperature was recorded every 10 seconds for an additional 60 seconds. From the results shown in Figure 6, it can be concluded that the absolute difference between the average temperature and the set temperature was no greater than 1.1°C, and the temperature curve consistently fluctuated around 37°C. This indicates that the temperature control system can achieve dynamic temperature regulation, capable of maintaining the temperature close to 37°C. However, this temperature control method generates relatively large temperature fluctuations.
 
 
-
 [![](https://static.igem.wiki/teams/5358/hardware/6.png) ![](https://static.igem.wiki/teams/5358/hardware/7.png)]{.flex .gap-4}
 
 [Figure 7 Measurement Results of  Initial Scheme's Temperature Stability]{.fig-title}
@@ -116,7 +98,6 @@ In response to the issues raised and the suggestions from the instructors, we ha
 
 [Figure 9 Improved Design Scheme]{.fig-title}
 
- 
 
 Firstly, we replaced the STM32F103C8T6 with the Arduino Nano ESP32 chip as the MCU. The ESP32 features built-in Bluetooth communication capabilities and a rich set of peripheral interfaces. It supports the CMake and Ninja build systems, which will greatly simplify the design complexity. Its widespread adoption globally will also make it more universal and laying a foundation for future teams looking to design similar devices. For temperature control and detection, we replaced the original scheme with the TCM1040 temperature control module and the FM-4A digital fluorescence detection module from LIDE Technology, respectively. Compared to the original scheme, these modules offer more integrated features, further enhancing the accuracy and stability of the platform.
 
@@ -130,7 +111,6 @@ To meet the demand for high-throughput detection, we proposed two solutions: a m
 
 [Figure 11 Implementation Plan of the New Equipment]{.fig-title}
 
- 
 
 ![](https://static.igem.wiki/teams/5358/engineering-success/new.png)
 
@@ -149,7 +129,6 @@ Temperature Control Capability Test
 
 We conducted another temperature stability test on the latest version of the system. The results shown in Figure 12 indicate that the absolute difference between the average temperature and the set temperature is less than 0.3°C, suggesting that the stability of the improved temperature control system has been further enhanced. This can well meet practical requirements.
 
-
  
 [![](https://static.igem.wiki/teams/5358/hardware/13.png) ![](https://static.igem.wiki/teams/5358/hardware/14.png)]{.flex .gap-4}
 
diff --git a/content/hp-ihp.md b/content/hp-ihp.md
index a5b540f3000dc5c963b347b690b6a403dc14933b..2a0777cd1b156f949ef221e04928b8f32c96aae5 100644
--- a/content/hp-ihp.md
+++ b/content/hp-ihp.md
@@ -10,7 +10,6 @@ The core of HP is to present project designs and products to the community and s
 ![](https://static.igem.wiki/teams/5358/ihp/brief.jpg)
 
 
-
 The "BRIEF" concept consists of five phases of IHP implementation, They are Brainstorming, Researching, Insight Integrating, Executing and Adjusting, and Feedback Recollecting five aspects. Among them, Executing and Adjusting the project is a bridge connecting Brainstorming, Researching, Insight Integrating and Feedback Recollecting. Throughout IHP, we build a cycle of finding meaning, reaching out, and connecting our projects to the world.
 
 ## Brainstorming
@@ -19,8 +18,6 @@ Our team is focusing on detecting new pollutants this year. At the beginning of
 
 Since then, endocrine disruptors have been screened according to the difficulty of detection, the harm to the environment and the significance of positive results. In the traditional evaluation system of endocrine disruptors, most of the main methods are to detect their binding with hormone receptors. However, due to the extensive and complex toxic effects of endocrine disruptors and their metabolites on organisms, the traditional detection methods are obviously inadequate.
 
-
-
 The result of our brainstorming is to build a gate-based multi-toxicological screening sensor that can simultaneously detect endocrine disrupting effects and DNA damage effects in factory and enterprise products, or unknown samples in the environment.
 
 ## Researching
@@ -39,8 +36,6 @@ In order to identify the social needs of our project with stakeholders, we conta
 On May 23, 2024, our team members approached Ms. Luo, an official of Chongqing Ecological Environment and Environmental Protection Bureau, to consult the social needs of our project.
 First of all, we consulted the definition method, detection and registration process and control measures of endocrine disruptors. Luo pointed out that after confirming that a substance is polluting, the government departments will commission professional technical institutions to conduct research and investigation every year, and if the result is ultra-ultra-trace, relatively safe, no further measures will be taken. But if the concentration is high, the government will take measures and test the water quality of the test site or its source to track its possible source. In the analysis of new pollutants, including endocrine disruptors, government departments will conduct toxicological tests on chemical substances based on cutting-edge international research to determine that a substance may be harmful to the human body. This is a very important part of the testing process, because the government departments will concentrate the samples, which will lead to a variety of substances will be detected, and only when these substances are associated with toxicological effects and confirmed to be harmful to the human body, it is necessary to define endocrine disruptors and monitor. In terms of control, the government has corresponding emission standards for well-known endocrine disruptors; For new pollutants such as endocrine disruptors detected, the government will continue to confirm whether they belong to the emission substances already on the control list. For the situation where the pollutant index exceeds the standard, the government department will require the enterprise to take corresponding treatment measures and implement management measures such as regular review in the follow-up to supervise the rectification results.
 
-
-
 As for endocrine disruptors, Luo pointed out that in some studies, all organic compounds were classified as endocrine disruptors under investigation, which is not accurate; The current approach is to study each emerging substance independently and then classify it. The test site of endocrine disruptors is generally in other important water bodies such as drinking water sources, livestock and poultry farms and chemical plants and other emission sources.
 
 Secondly, we proposed a method to determine whether the water quality contains endocrine disruptors by using the biosensor constructed by the engineering bacteria that specifically recognizes endocrine disruptors. Luo first confirmed that in the environmental field, the emergency equipment launched abroad in the past few years does include microbial sensors and fluorescence detection technology to deal with sudden emergency environmental accidents. For endocrine disruptors, the government does not include them in routine testing tasks, but they are regularly included in testing efforts. The government prefers to use more accurate tests to detect endocrine disruptors. Therefore, from this point of view, improving the accuracy and sensitivity of the detection is also very beneficial and important.
@@ -57,8 +52,6 @@ In order to continue to understand the definition method and testing registratio
 
 Guo pointed out that the determination of pollutants is first based on those already found by the country, and their emissions often exceed the prescribed standards, while classification and the formulation of standards are needed. The study of these pollutants needs to determine whether they have harmful effects on the human body through effect analysis, toxicology analysis and process analysis. Secondly, the use of foreign literature research combined with the actual situation in China to develop a standardized detection process to find endocrine disruptors. The definition of most new pollutants, especially endocrine disruptors, is that they are first found to be harmful to human health, and then they are analyzed in detail.
 
-
-
 For the analysis standards of new pollutants such as endocrine disruptors, Guo mentioned that with the development of industry, some new substances will be continuously studied, but whether it is harmful to the human body needs to be determined through a scientific judgment process, and if it is found to be harmful to the human body, it needs to be immediately included in the supervision. According to the requirements of the state, Shanghai has put the new pollutants to be studied into the supervision. Both testing organizations and industry systems are constantly developing methods to detect new pollutants, including endocrine disruptors, and current detection instruments are sophisticated and expensive.
 
 In the subsequent improvement process, the hardware device has undergone a simplified design to achieve the above design goals of portability and miniaturization.
@@ -67,8 +60,6 @@ In the communication with Luo and Guo, they both affirmed our thinking and impor
 
 ### Factory and Enterprise
 
-
-
 Our next step is to understand the current status of the detection and analysis of endocrine disruptors from the perspective of relevant units and companies, as well as the specific needs currently existing, and ask whether there are applicable scenarios for the project. In accordance with Luo's previous guidance, we found a sewage plant and a water plant in Shanghai on August 1, 2024.
 
 #### Shanghai Taihe Wastewater Treatment Plant
@@ -89,8 +80,6 @@ Therefore, for factories and enterprises, our project has a demand in the rapid
 
 In short, the need for endocrine disruptor testing that we investigated is actually confirmed. At present, the detection of new pollutants including endocrine disruptors is more passive, and the method is more heavy on toxicological analysis, and has high cost and cumbersome operation. Therefore, there is a real need for a highly sensitive and specific detection of endocrine disruption and DNA damage in unknown samples, two important toxicological effects, and the hardware of such sensors must be portable and miniaturized.![](https://static.igem.wiki/teams/5358/ihp/projectpropose.png)
 
-
-
 ### Professional research personnel
 
 ![](https://static.igem.wiki/teams/5358/ihp/yang.jpg)
@@ -99,11 +88,8 @@ We had an in-depth discussion on Modeling with Professor Yang Guanghui of China
 
 Inspired by this, and after subsequent investigation, we will use Chimera1.13 software to analyze the hydrophobic interaction of the protein conformation obtained by molecular dynamics simulation (MDS), and measure the hydrophobic interaction intensity by comparing the hydrophobic potential around the recAN end and the C-end, so as to compare the degree of folding of this domain in the protein
 
-
 ## Feedback Recollecting
 
-
-
 We plan to bring the prototype design to endocrine disruptor experts and previous stakeholders, and continue to receive their comments and feedback to continue to adapt our project; At the same time, we will also find new innovation points and needs from the feedback, so as to launch a new round of "BRIEF" cycle in the future and complete the closed loop.
 
 ![](https://static.igem.wiki/teams/5358/ihp/executingandadjusting.png)
diff --git a/content/models.md b/content/models.md
deleted file mode 100644
index 52ff63e79538bd892a724ce5d98b9e6ab28f81ab..0000000000000000000000000000000000000000
--- a/content/models.md
+++ /dev/null
@@ -1,48 +0,0 @@
-## Models
-
-### Overview
-
-在我们的设计中，我们的项目主要分为建立基于门控的微生物传感器和便携易使用的小型化设备。我们将主题分为三个层次：基因、蛋白质和应用，并为三种不同情况建立了数学模型：在基因层面主要通过Hill方程……；在蛋白质模型中我们先预测了每种可能性的蛋白质结构模型，再通过多种软件工具进行综合考虑，选择出最优插入方案；在应用层面利用comsol软件对于微控流芯片的设计构型进行模拟，辅助做出判断。
-
-
-
-#### 基因层模型：
-
-为了进一步探索本项目设计的遗传电路的内在机制，对复杂的响应系统进行合理的分析和预测，并能够对实验提供一些反馈和建议，我们建立了数学模型，并利用多个软件工具来指导实验获取用于拟合模型的数据，并进一步优化模型。这可以为我们的项目提供更多的灵感。
-在本模块中，我们的最终目标是验证BsFbFp基因可以在无氧环境下有效表达荧光和实现与门线路的协同检测的可行性，并从基因成分的角度给出遗传回路的优化方向。为了达到这一目的，我们的主要工作分为两部分……得出了……的结论，为
-
-
-
-
-
-### 蛋白质模型：
-
-我们设计的生物传感器使用了来自结核分枝杆菌 (Mtu)的内含肽RecA。我们想将其放入我们的工程菌中，其中我们需要稳定的RecA intein并将其插入T7 RNAP中，以检测内分泌干扰效应。这就提出了一个有趣的问题，即“我们将RecA插入在哪里才能得到最高效稳定的T7RNAP-intein？“这为使用 Dry Lab 来扩展我们在 Wet Lab 中所做的工作提供了机会，因为鉴于没有评价蛋白质效果的统一方法，我们决定模拟 T7 RNAP的每种可能的插入位点所形成的蛋白质，以及从与雌二醇的结合效果、静电势分析、能量分析等角度综合评估选择最优蛋白质
-
-最优蛋白质结构分析
-
-1.蛋白质结构预测
-
-查阅资料得知，RecA可能的插入位点集中在半胱氨酸旁，于是根据T7 RNAP的肽链得到十二种可能的工程蛋白T7RNAP-intein。并使用alphafold3来进行结构预测。同时作为参照采取……的同源建模方式加以佐证
-
-2.雌激素结合效果
-
-
-
-3.静电势分析
-
-pymol的APBS插件能够分析并计算电势分布，而电势分布较为均匀的蛋白质
-
-
-
-4.能量分析
-
-
-
-### 微控流芯片建模
-
-
-
-
-
-### reference
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diff --git a/content/project-engineering.md b/content/project-engineering.md
index 8d73f7644168989e7d4d85c0b961cd6204b471ad..816d1e65bbf691abdff9c60e702d04cd2ebe7656 100644
--- a/content/project-engineering.md
+++ b/content/project-engineering.md
@@ -16,7 +16,7 @@
 [Figure 1. BsFbFp circuits]{.fig-title}
 
 
-​	Genetic circuit: Gene recA and BsFbFp were all synthesized by Sangon Biotech according to BIOBRICK standard, and the gene circuit was obtained by PCR and verified by agarose gel electrophoresis.
+​Genetic circuit: Gene recA and BsFbFp were all synthesized by Sangon Biotech according to BIOBRICK standard, and the gene circuit was obtained by PCR and verified by agarose gel electrophoresis.
 
 
 
@@ -128,7 +128,8 @@ Construction of plasmid: In the multi-round adjustment experiments of the ligati
 
 ​
 The agarose gel electrophoresis results showed that after redesigning the PCR primers and increasing the number of protective bases on both sides of the restriction enzyme cutting sites from one or none to three, only one band was observed for the target fragment, indicating that the new PCR primers have strong specificity and the target fragment was successfully obtained.
-​	However, due to time constraints, we did not proceed with the subsequent experiments.
+​
+However, due to time constraints, we did not proceed with the subsequent experiments.
 
 ##### Test
 
@@ -173,7 +174,7 @@ In terms of material selection, we have adopted a black silicone kit and an alum
 
 #### Establishment
 
-​	The system construction utilized common integrated modules, with the main controller being an ESP32 chip, and temperature control and detection were accomplished using the TCM temperature control module and LIDE Technology's FM series digital fluorescence detection module, respectively. We employed industrial processing techniques to fabricate the aluminum alloy temperature-controlled slots, while the casing and other components were created using 3D printing technology.
+​The system construction utilized common integrated modules, with the main controller being an ESP32 chip, and temperature control and detection were accomplished using the TCM temperature control module and LIDE Technology's FM series digital fluorescence detection module, respectively. We employed industrial processing techniques to fabricate the aluminum alloy temperature-controlled slots, while the casing and other components were created using 3D printing technology.
 
 
 ![Schematic Diagram of Hardware Equipment](https://static.igem.wiki/teams/5358/engineering-success/new.png)
@@ -187,7 +188,7 @@ In terms of material selection, we have adopted a black silicone kit and an alum
 
 #### Test
 
-​	After finalizing the initial plan, we first carried out feasibility tests to verify the functionality of the fluorescence system and the temperature control system. After assembling the initial version of the model, numerous issues were identified through usage testing and preliminary risk analysis, such as the susceptibility of the fluorescence system to interference and the instability of the temperature control system. Through a cycle of testing, feedback, improvement, and retesting, the final version of the model demonstrated excellent performance across all aspects. Experiments showed that fluorescence identification was highly accurate, and the overall aesthetics were significantly enhanced.
+​After finalizing the initial plan, we first carried out feasibility tests to verify the functionality of the fluorescence system and the temperature control system. After assembling the initial version of the model, numerous issues were identified through usage testing and preliminary risk analysis, such as the susceptibility of the fluorescence system to interference and the instability of the temperature control system. Through a cycle of testing, feedback, improvement, and retesting, the final version of the model demonstrated excellent performance across all aspects. Experiments showed that fluorescence identification was highly accurate, and the overall aesthetics were significantly enhanced.
 
 
 ![Results of Temperature Control Experiment](https://static.igem.wiki/teams/5358/engineering-success/results-of-temperature-control-experiment.png)
@@ -201,7 +202,7 @@ In terms of material selection, we have adopted a black silicone kit and an alum
 
 #### Learn
 
-​	During the process of designing the device, we encountered various challenges, such as overly complex control systems, insufficient stability, inability to conduct multi-sample testing, and issues with the optical path design. Throughout the real-time testing and discussion phases, we continuously documented various issues, proposed improvement plans, and actively sought help from knowledgeable teachers and consultants. Eventually, we completed a hardware design that met the requirements. Through continuous learning, improvement, and questioning, our expertise in the relevant fields has been greatly boosted.
+​During the process of designing the device, we encountered various challenges, such as overly complex control systems, insufficient stability, inability to conduct multi-sample testing, and issues with the optical path design. Throughout the real-time testing and discussion phases, we continuously documented various issues, proposed improvement plans, and actively sought help from knowledgeable teachers and consultants. Eventually, we completed a hardware design that met the requirements. Through continuous learning, improvement, and questioning, our expertise in the relevant fields has been greatly boosted.
 
 ## Software
 
diff --git a/content/team-contribution.md b/content/team-contribution.md
new file mode 100644
index 0000000000000000000000000000000000000000..ba827289098b9ae88e2a4b3a8a811bbefd657ee1
--- /dev/null
+++ b/content/team-contribution.md
@@ -0,0 +1,121 @@
+---
+title: Contribution
+---
+
+## Abstrct
+
+| Type     | Introduction                                                 |
+| -------- | ------------------------------------------------------------ |
+| Part     | Update experimental data of existing part                    |
+| Hardware | Provide modeling files and size parameters of hardware devices |
+| Software | Provide the source code of software                          |
+| HP       | Provide a novel and integrated concept to conduct human practice |
+| model    | Provide protein model and                                    |
+| design   | Provide editable illustration materials designed by team BIT |
+
+## Part：Experimental data 
+
+This year, Team BIT is committed to developing a synergistic toxicological assessment system for endocrine disruptors and DNA-damaging substances. The core goal is to develop a highly sensitive microbial sensor. The principle of the sensor's response to DNA damage is based on the intein's cleavage and the bacterial SOS response, which initiates downstream transcriptional activity and expresses fluorescent proteins. We will collaboratively develop portable detection hardware to achieve efficient and portable detection outdoors. The hardware will provide an approximately anaerobic environment to ensure stable characterization under anoxic conditions. To ensure sufficient fluorescence intensity while achieving a high limit of detection, we have used BsFbFp as the final characterization signal and registered part [BBa_K5358010](https://parts.igem.org/Part:BBa_K5358010).
+
+See more in [**result**](https://2024.igem.wiki/bit/result)
+
+## **Hardware :Modeling files and Size parameters**
+
+This year's BIT team has developed a high-throughput EDCs detection platform characterized by its compact, portable, and highly integrated design, demonstrating excellent performance in testing. We will provide detailed insights into the hardware design philosophy, structural construction, and implementation methods. Additionally, we will make all parts' SolidWorks 3D models publicly available, hoping that other teams can replicate our product or draw some inspiration from it.
+
+### **Design Philosophy:** 
+
+The goal of the hardware device is to construct a platform that includes a reaction vessel, temperature control, and fluorescence detection. To achieve this, we adopted the typical engineering and project design approach as shown in Figure 1, underwent several design iterations, and ultimately selected an integrated, high-throughput solution.
+
+ 
+
+![img](https://static.igem.wiki/teams/5358/contribution/hardware-fig1.png)
+
+<div style="text-align: center;">
+Figure 1 Hardware Design Flowchart 
+</div>
+
+### **Structural Construction:**
+
+The final structure is primarily divided into a sampling area and a detection area.
+
+ 
+
+l Sampling Area: An aluminum alloy temperature-controlled slot houses multiple reaction tubes, with temperature control achieved through a temperature control module.
+
+l Detection Area: A linear slide rail drives the fluorescence detector to sequentially inspect each reaction tube, enabling high-throughput detection. 
+
+The functions of each module are centrally controlled using an MCU chip.
+
+ 
+
+![img](https://static.igem.wiki/teams/5358/contribution/hardware-fig2.png) 
+
+<div style="text-align: center;">
+Figure 2 Hardware Structure Diagram
+</div>
+### **System Implementation:**
+
+The main components selected for the hardware device are shown in the table below:
+
+|                                  | ***components*** |
+| -------------------------------- | -------------------- |
+| ***MCU***                    | Arduino Nano ESP32   |
+| ***Temperature Control***    | TCM1040              |
+| ***Temperature Sensor***     | NTC                  |
+| ***Heat Source***            | Heating FILM         |
+| ***Fluorescence Detection*** | FM-4A                |
+| ***Reaction Platform***      | Slide Rail Platform  |
+| ***Motor Drive***            | 2830 Stepper Motor   |
+ 
+
+ 
+
+![img](https://static.igem.wiki/teams/5358/contribution/hardware-fig3.png) 
+
+<div style="text-align: center;">
+Figure 3 Final Model Diagram
+</div>
+Please contact us via the email address at the bottom to obtain all component models and the complete modeling files!
+
+## **Software :The Source Code**
+
+The software portion of this project involved the design of a mobile app that leverages Bluetooth communication to automate control of the hardware device and to acquire fluorescence signals in real-time. The signals, after data processing, yield detection results that are then visualized.
+
+ 
+
+The project's software is written in QT. Please contact us  to obtain the source code!
+
+
+
+## Human Practice：New concept of Conducting HP 
+
+### “BRIEF”cycle for conducting HP
+
+![](https://static.igem.wiki/teams/5358/ihp/brief.jpg)
+
+This year, we innovatively put forward the "BRIEF" cycle to guide the development of Human Practice. This system divides HP's work into five phases: Brainstorming, Researching, Insight Integrating, Executing and Adjusting, and Feedback recollecting. iGEMer will propose a project with both research and practical significance through Brainstorming, and conduct in-depth investigation on the relevant field through Researching. After Insight Integrating, Executing and Adjusting, Feedback Recollecting, a logical closed loop will be formed, putting forward ideas, searching for significance, seeking external insights, and constantly connecting our project with the world.
+
+See more in **Human-practices**
+
+## Model: Protein Model
+
+In our bio-detection project this year, we've integrated a hormone-sensitive intein into T7 RNA polymerase to create a recognition element for environmental endocrine disruptors (EDCs). The intein is inserted following a cysteine residue at the C-terminus of the host protein. With 12 cysteines in T7 RNA polymerase, this provides 12 potential insertion sites.
+
+To analyze all possible insertions, we first modeled the protein with the intein at each of the 12 sites. These models serve as a reference for other iGEMers. We then analyzed these models from various perspectives: molecular docking, spatial folding, protein SASA (Surface Area Solvent Accessible) analysis, and electrostatic potential analysis. These processes offer a roadmap for other iGEMers in protein analysis work.
+
+For spatial folding analysis, we wrote a script to identify hydrogen bonds in proteins. The script is available on our wiki for any iGEMers who need it.
+
+See more in [**Model**](https://2024.igem.wiki/bit/model)
+
+## Design: Editable Illustration Materials
+
+This year, our graphic designer, Li Sixuan, has created a series of wiki elements with a unique style, including animations for the home page as well as borders and decorations for each interface. These wiki elements can be referred to by all iGEMers and can be downloaded at any time from our website.
+
+![](https://static.igem.wiki/teams/5358/contribution/art3.png)
+
+
+
+![](https://static.igem.wiki/teams/5358/contribution/art2.png)
+
+![](https://static.igem.wiki/teams/5358/contribution/home-3.png)
diff --git a/content/wetlab-experiment.md b/content/wetlab-experiment.md
index 19fb3587bac413cc87075210a544127037536f65..b0a35b5eab99ced1a338b7c59743b84d1fee0b53 100644
--- a/content/wetlab-experiment.md
+++ b/content/wetlab-experiment.md
@@ -35,7 +35,6 @@ Protocol
 - The product is stored at -10°C;
 
 
-
 ##### **Agarose gel electrophoresis and sample recovery**
 
 Material
@@ -92,7 +91,6 @@ Protocol
 
 After adding the following enzyme digestion system to a 0.2 mL centrifuge tube, incubate it in an incubator at 37°C for 2 h.
 
- 
 
 | Enzyme digestion system | Volume |
 | ----------------------- | ------ |
@@ -157,11 +155,11 @@ Transfer The bacteria solution cultured overnight to 30mL fresh non-resistant LB
 Method 1：The bacterial solution was added to DNA damaging agent, dropped into anaerobic row and incubated for 2h in a 37 C incubator. Measure OD600 and fluorescence intensity of bacterial solution by microplate reader;
 
 Method 2：The bacterial solution was sealed using paraffin oil and DNA damaging agents were added under the paraffin oil page and incubated for 2h in a 37 C incubator. Measure OD600 and fluorescence intensity of bacterial solution by microplate reader;
- 
 
-| DNA damaging reagent | Concentration gradient |      |      |      |      |      |      |
-| -------------------- | ---------------------- | ---- | ---- | ---- | ---- | ---- | ---- |
-| NA                   | 0μL                    | 1μL  | 5μL  | 10μL | 15μL | 20μL | 25μL |
+
+| DNA damaging reagent | Concentration gradient |      |
+| -------------------- | ---------------------- | ---- |
+| NA                   | 0μL                    | 1μL 5μL 10μL 15μL 20μL 25μL| 
 
 
 
@@ -177,8 +175,6 @@ Transfer 200μL of the bacteria solution to a 96-hole black microplate and place
 Transfer 200μL of the bacteria solution to a 96-hole black microplate and place it in a microplate reader. Set the excitation wavelength to 488 nm, emission wavelength to 520 nm, and gain to 75. Measure the fluorescence intensity.
 
 
-
 Each group of data was measured three times and averaged.
 
-
  
\ No newline at end of file
diff --git a/content/wetlab-parts.md b/content/wetlab-parts.md
index c1e91fbd80b07b9cf4c0b8fb9d31ef4eb9dbd825..23d9a4a34c65d384fafa8e0d6d42c2792c36405b 100644
--- a/content/wetlab-parts.md
+++ b/content/wetlab-parts.md
@@ -85,4 +85,3 @@ Our part successfully detected DNA damage and endocrine disruptors caused by the
 | BBa_R0062    | Regulatory        | The inducible promoter in lux gene regulatory network        | 55bp   |
 | BBa_C0062    | Coding            | The activator protein luxR in lux gene regulatory network    | 781bp  |
 
-
diff --git a/content/wetlab-results.md b/content/wetlab-results.md
index 0136dc83fe6b553292a618545bc206a9f165d6fd..1c3eea7b551eb76895e3addfd7596a07a10e642b 100644
--- a/content/wetlab-results.md
+++ b/content/wetlab-results.md
@@ -44,7 +44,6 @@ Later, we compared the characterization difference between DH5α strain and BL21
 
 [Figure 4. Fluorescence characterization gradients of DH5α and BL21 under aerobic and NA gradient injury conditions.]{.fig-title}
 
- 
 
 ## 2. pET28-PrecA-hrpR-hrpS-PhrpL-BsFbFp-BL21
 
@@ -62,7 +61,7 @@ The gene circuits were ligated to the pET28 vector using enzyme ligation and tra
 
 The gene sequencing conducted by our company has successfully validated the synthesis of the intein of the biological circuit.
 
- 
+
 ![img](https://static.igem.wiki/teams/5358/experiment/fig3.png) 
 
 ![img](https://static.igem.wiki/teams/5358/experiment/gene-squencing.png) 
@@ -88,20 +87,3 @@ We obtained by PCR the Pj233100, Plux-LuxR, LuxI, and EGFP and ligated it to the
 
 [Figure 9. Enzyme Ligation Product of psB1C3-Pj23100-LuxI-BL21 and Plux-LuxR-EGFP-BL21.]{.fig-title}
 
- 
-
- 
-
- 
-
- 
-
- 
-
- 
-
- 
-
- 
-
- 
\ No newline at end of file