diff --git a/src/contents/engineering.tsx b/src/contents/engineering.tsx index 08867fa43cbd95918e8c32d7f95aec9bee0f52e4..5c49713c2673667b12515dcf6ec762b0474fb29d 100644 --- a/src/contents/engineering.tsx +++ b/src/contents/engineering.tsx @@ -242,19 +242,19 @@ export function Engineering() { <p> The PreCyse cassette comes in three versions: PreCyseA, the most basic version, comprises of a T7 promoter and an open reading frame, which includes NLS and one typeIIS restriction enzyme cloning site for a nickase and a reverse transcriptase each. For possible future additions like e. g. selection markers, a BamHI restriction site at the end of the coding sequence allows for easy in-frame Gibson cloning. Building on this basis, PreCyseB expands PreCyseA by the La Poly(U)-binding motif. PreCyseC additionally introduces the co-expressed MLH1dn. The cassettes were ordered in three individual parts to be put together with a pCMV-PE6c backbone via Gibson Cloning in different configurations to create the three variants. In the plasmid the cassette is expressed under a CMV promoter and followed by a polyadenylation signal. The PreCyse Casettes themselves can be used as a BioBrick RFC[10] standard compatible composite part can thus be freely combined with other parts. The nickase and RT slots can be used for inserting any basic or composite part compatible with the Type IIS RCF[1000] standard for fusion proteins. The PreCyse Casette is meant to be a contribution to the iGEM community and a base for other teams to join us and researchers around the world to innovate in the exciting field of prime editing. </p> - <div className='row align-items-top'> - <div className='col align-items-center'> + <div className="row align-items-center"> + < div className='col align-items-center'> <H5 text="PreCyseA" id="PreCyseA"/> - <img src="https://static.igem.wiki/teams/5247/engineering-cycle/precysea-casette.svg" alt="PreCyseA modular PE casette" style={{height: "80pt"}}/> + <img src="https://static.igem.wiki/teams/5247/engineering-cycle/precysea-casette.svg" alt="PreCyseA modular PE casette" style={{height: "50%", width: "auto"}}/> </div> <div className='col align-items-center'> <H5 text="PreCyseB" id="PreCyseB"/> - <img src="https://static.igem.wiki/teams/5247/engineering-cycle/precyseb-casette.svg" alt="PreCyseB modular PE casetter" style={{height: "80pt"}}/> + <img src="https://static.igem.wiki/teams/5247/engineering-cycle/precyseb-casette.svg" alt="PreCyseB modular PE casetter" style={{height: "50%", width: "auto"}}/> </div> - </div> - <div className='row align-items-center'> + <div className='row align-items-center'> <H5 text="PreCyseC" id="PreCyseC"/> - <img src="https://static.igem.wiki/teams/5247/engineering-cycle/precysec-casette.svg" alt="PreCyseC modular PE casette" style={{height: "80pt"}}/> + <img src="https://static.igem.wiki/teams/5247/engineering-cycle/precysec-casette.svg" alt="PreCyseC modular PE casette" style={{height: "50%", width: "auto"}}/> + </div> </div> {/* <H4 text="Test" id="text"/> <p></p> @@ -519,7 +519,7 @@ export function Engineering() { <p>We collaborated with <a onClick={() => goToPagesAndOpenTab('corden', '/human-practices')}>Corden Pharma</a>, a specialist in LNP technologies, to address these concerns. Based on their recommendations, we opted for <strong>mPEG-2000-DSPE</strong> as our PEG-lipid of choice. This variant minimizes cytotoxicity while providing excellent stability and circulation time. It has also proven effective in reducing immune-related side effects while preserving the integrity and performance of our nanoparticles. </p> <H4 text="DMG-PEG2000 vs mPEG-2000-DSPE" id="text" /> While mPEG-2000-DSPE has traditionally been used for stabilizing LNPs and enhancing delivery efficiency, we decided to incorporate DMG-PEG2000 into our SORT LNP-based AirBuddy due to its superior properties. DMG-PEG2000 offers better biodegradability and enhanced stability in pulmonary applications. Unlike mPEG-2000-DSPE, which tends to accumulate in the body and may lead to immune activation over time, DMG-PEG2000 is known for its faster clearance and reduced potential for long-term toxicity. For lung-specific delivery, where stability and safety are critical, DMG-PEG2000 ensures that the nanoparticles remain stable long enough to deliver the therapeutic material effectively, but also degrade at a rate that minimizes unwanted immune responses. This makes DMG-PEG2000 a more suitable choice for therapies targeting CFTR-related diseases, where precise and safe delivery to the lungs is essential for treatment success. - <p>Details about the biosafety aspects of our LNP can be read // <a onClick={() => goToPageAndScroll ('Biosafety2H', '/safety')}> here </a>. </p> + <p>Details about the biosafety aspects of our LNP can be read <a onClick={() => goToPageAndScroll ('sort-lnp-and-cytotoxicity', '/safety')}> here </a>. </p> <p>Click this Button to gain the LNP Handbook</p> <DownloadLink url="https://static.igem.wiki/teams/5387/liposomes-handbook.pdf" fileName="liposomes-handbook.pdf" />