From ea6332b90ab32e98e0396c0dda9308b303089dc9 Mon Sep 17 00:00:00 2001
From: Liliana Sanfilippo <liliana.sanfilippo@uni-bielefeld.de>
Date: Fri, 27 Sep 2024 13:24:31 +0200
Subject: [PATCH] Video Patchclamp
---
src/contents/methods.tsx | 5 +----
1 file changed, 1 insertion(+), 4 deletions(-)
diff --git a/src/contents/methods.tsx b/src/contents/methods.tsx
index b9692eab..bc9f3aa2 100644
--- a/src/contents/methods.tsx
+++ b/src/contents/methods.tsx
@@ -15,10 +15,7 @@ export function Methods() {
<H4 text="Principles of the patch clamp technique"></H4>
<p>Patch clamp recording involves the use of a glass micropipette which is manufactured from a glass capillary through the use of a Micropipette Puller. The micropipette is then filled with an electrolyte solution, which is subsequently brought into contact with the cell membrane. By applying gentle suction, a high-resistance seal called giga seal is formed between the pipette tip and the membrane patch. This enables the measurement of ionic currents with minimal noise interference [3]. <strong>Whole-Cell Configuration</strong> records currents from the entire cell by rupturing the membrane patch, accessing the intracellular environment, and is useful for analysing overall ion channel activity and cellular responses. <strong>Single-Channel Recording</strong> measures currents through individual ion channels without rupturing the membrane, enabling high-resolution study of channel conductance, gating, and selectivity [2].</p>
<figure>
- <video controls>
- <source src="https://video.igem.org/w/2FggDvrNQNUH5CTtrzFR38" type="video/mp4"></source>
- <source src="https://video.igem.org/w/2FggDvrNQNUH5CTtrzFR38" type="video/webm"></source>
- </video>
+ <iframe title="Bielefeld-CeBiTec: Patch Clamp Measurement (2024)" width="560" height="315" src="https://video.igem.org/videos/embed/0d948e57-5997-430a-a2df-815b71a2fc67?autoplay=1" frameBorder="0" allowFullScreen={true} sandbox="allow-same-origin allow-scripts allow-popups allow-forms"></iframe>
<figcaption>microscopic recording of micropipette sealing of a HEK293 cell </figcaption>
</figure>
--
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