diff --git a/src/contents/methods.tsx b/src/contents/methods.tsx index b9692eab8f4103fe922ea996099cb88e665e2b44..bc9f3aa2859b1f832a9bc38c01ae1f4fcc63f6de 100644 --- a/src/contents/methods.tsx +++ b/src/contents/methods.tsx @@ -15,10 +15,7 @@ export function Methods() { <H4 text="Principles of the patch clamp technique"></H4> <p>Patch clamp recording involves the use of a glass micropipette which is manufactured from a glass capillary through the use of a Micropipette Puller. The micropipette is then filled with an electrolyte solution, which is subsequently brought into contact with the cell membrane. By applying gentle suction, a high-resistance seal called giga seal is formed between the pipette tip and the membrane patch. This enables the measurement of ionic currents with minimal noise interference [3]. <strong>Whole-Cell Configuration</strong> records currents from the entire cell by rupturing the membrane patch, accessing the intracellular environment, and is useful for analysing overall ion channel activity and cellular responses. <strong>Single-Channel Recording</strong> measures currents through individual ion channels without rupturing the membrane, enabling high-resolution study of channel conductance, gating, and selectivity [2].</p> <figure> - <video controls> - <source src="https://video.igem.org/w/2FggDvrNQNUH5CTtrzFR38" type="video/mp4"></source> - <source src="https://video.igem.org/w/2FggDvrNQNUH5CTtrzFR38" type="video/webm"></source> - </video> + <iframe title="Bielefeld-CeBiTec: Patch Clamp Measurement (2024)" width="560" height="315" src="https://video.igem.org/videos/embed/0d948e57-5997-430a-a2df-815b71a2fc67?autoplay=1" frameBorder="0" allowFullScreen={true} sandbox="allow-same-origin allow-scripts allow-popups allow-forms"></iframe> <figcaption>microscopic recording of micropipette sealing of a HEK293 cell </figcaption> </figure>