diff --git a/src/contents/safety.tsx b/src/contents/safety.tsx index 38e85969535fd28c4e1e45b4aec8eaf69279280c..609c68957de2297ff657423aecd85d9c959d82fc 100644 --- a/src/contents/safety.tsx +++ b/src/contents/safety.tsx @@ -4,20 +4,21 @@ import { LoremMedium } from "../components/Loremipsum"; import PreCyse from "../components/precyse"; import { SupScrollLink } from "../components/ScrollLink"; import { Section, Subesction } from "../components/sections"; -import { useTabNavigation } from "../utils/TabNavigation"; import Collapsible from "../components/Collapsible"; +import { useNavigation } from "../utils"; // message for test commit. export const Safety: React.FC = () =>{ - useTabNavigation(); + const {goToPageAndScroll} = useNavigation(); + return ( <> <Section title="Role in iGEM" id="Role"> <p> - As part of our project <PreCyse/> to develop a prime-editing complex to correct the F508del mutation in cystic fibrosis, we place great emphasis on safety at all stages of research. Our final construct will be tested in <a href="methods?scrollTo=Cell Culture3">primary cultures of epithelial cells</a> obtained from nasal swabs, isolated from both patients and healthy individuals. To guarantee safety and ensure the highest level of precision and reliability of our results, we have introduced a series of carefully planned checkpoints during the experiments. These milestones allow for continuous monitoring, timely adjustments and validation at each critical stage. This ensures that potential issues are identified and addressed immediately, minimizing risk and improving the overall quality of the experimental results. + As part of our project <PreCyse/> to develop a prime-editing complex to correct the F508del mutation in cystic fibrosis, we place great emphasis on safety at all stages of research. Our final construct will be tested in <a onClick={() => goToPageAndScroll ('Cell Culture3H', '/materials-methods')}> primary cultures of epithelial cells </a> obtained from nasal swabs, isolated from both patients and healthy individuals. To guarantee safety and ensure the highest level of precision and reliability of our results, we have introduced a series of carefully planned checkpoints during the experiments. These milestones allow for continuous monitoring, timely adjustments and validation at each critical stage. This ensures that potential issues are identified and addressed immediately, minimizing risk and improving the overall quality of the experimental results. </p> </Section> <Section title="Check-Ins" id="Check-Ins"> @@ -43,7 +44,7 @@ export const Safety: React.FC = () =>{ <Section title="Check-Ins" id="Check-Ins"> <div> <p> - As part of our project to develop a prime-editing complex to correct the F508del mutation in cystic fibrosis, we place great emphasis on safety at all stages of research. Our final construct will be tested in <a href="methods?scrollTo=Cell Culture3">primary cultures of epithelial cells</a> obtained from nasal swabs, isolated from both patients and healthy individuals. To guarantee safety and ensure the highest level of precision and reliability of our results, we have introduced a series of carefully planned checkpoints during the experiments. These milestones allow for continuous monitoring, timely adjustments and validation at each critical stage. This ensures that potential issues are identified and addressed immediately, minimizing risk and improving the overall quality of the experimental results. [link zu den Experimenten] . iGEM places great emphasis on biosafety, ensuring that all projects adhere to strict safety standards. One of these measures is the iGEM White List, which includes organisms and parts that are pre-approved for use based on their safety profile. Any components or organisms not covered by this White List must be submitted as 'Check-Ins' to the iGEM Safety Committee for approval. Check-Ins are formal safety evaluations that allow the committee to assess the potential risks and ensure proper containment and handling procedures are in place. Although we used some parts and organisms that were not included on the White List, these were assessed as critical for our project and submitted as Check-Ins to the iGEM Safety Committee. Furthermore, we were in active exchange with the committee throughout the process. The Check-ins provide a clear picture of the biosafety aspects of our project, reflecting our commitment to safety and compliance with iGEM standards. + As part of our project to develop a prime-editing complex to correct the F508del mutation in cystic fibrosis, we place great emphasis on safety at all stages of research. Our final construct will be tested in <a onClick={() => goToPageAndScroll ('Cell Culture3H', '/materials-methods')}> primary cultures of epithelial cells </a> obtained from nasal swabs, isolated from both patients and healthy individuals. To guarantee safety and ensure the highest level of precision and reliability of our results, we have introduced a series of carefully planned checkpoints during the experiments. These milestones allow for continuous monitoring, timely adjustments and validation at each critical stage. This ensures that potential issues are identified and addressed immediately, minimizing risk and improving the overall quality of the experimental results. [link zu den Experimenten] . iGEM places great emphasis on biosafety, ensuring that all projects adhere to strict safety standards. One of these measures is the iGEM White List, which includes organisms and parts that are pre-approved for use based on their safety profile. Any components or organisms not covered by this White List must be submitted as 'Check-Ins' to the iGEM Safety Committee for approval. Check-Ins are formal safety evaluations that allow the committee to assess the potential risks and ensure proper containment and handling procedures are in place. Although we used some parts and organisms that were not included on the White List, these were assessed as critical for our project and submitted as Check-Ins to the iGEM Safety Committee. Furthermore, we were in active exchange with the committee throughout the process. The Check-ins provide a clear picture of the biosafety aspects of our project, reflecting our commitment to safety and compliance with iGEM standards. The main safety measures we have implemented include: </p> <p> @@ -96,7 +97,7 @@ export const Safety: React.FC = () =>{ <H4 text="Check-in for Testing in cell lines "></H4> <Collapsible id="CheckcellLines" open={false} title="open to see full results"> <p> - In our project, we paid attention to safety at every step, especially when working with specific <a href="methods?scrollTo=Cell Culture">cell lines</a>. All experiments were performed under strict S1 conditions, following all relevant safety protocols. Given the sensitivity of the human cell lines we used, we placed great emphasis on controlled and well-designed workflows. All transfections were performed in our own transfection laboratory to ensure a high level of safety and compliance. Below you will find an overview of the cell lines submitted by us as a checkin and the specific safety measures: + In our project, we paid attention to safety at every step, especially when working with specific <a onClick={() => goToPageAndScroll ('cell-culture', '/materials-methods')}> cell lines </a> . All experiments were performed under strict S1 conditions, following all relevant safety protocols. Given the sensitivity of the human cell lines we used, we placed great emphasis on controlled and well-designed workflows. All transfections were performed in our own transfection laboratory to ensure a high level of safety and compliance. Below you will find an overview of the cell lines submitted by us as a checkin and the specific safety measures: </p> <p> <strong>HEK293 cell line: </strong>HEK 293 (Human Embryonic Kidney 293) cells are an immortal cell line originally derived from the kidney cells of a human embryo. They are characterized by their fast division rate and high transfection efficiency, which makes them a popular model in biomedical research. For our studies, the basic HEK293 cells were provided to us by the Cellular and Molecular Biotechnology Group at Bielefeld University, headed by Prof. Dr. Kristian Müller. Prof. Dr. Müller is also one of the Principal Investigators of our team. We use this cell line in our proof-of-concept studies and for testing the Prime Editing Guide pegRNA (pegRNA) to evaluate the efficiency and functionality of our constructs.