From d4d27192bf6096eca7ace624a7c3bd2d53781a2c Mon Sep 17 00:00:00 2001
From: Liliana Sanfilippo <lsanfilippo@techfak.uni-bielefeld.de>
Date: Mon, 2 Dec 2024 18:01:05 +0100
Subject: [PATCH] Center Patch Clamp Video
---
src/contents/methods.tsx | 6 +++++-
1 file changed, 5 insertions(+), 1 deletion(-)
diff --git a/src/contents/methods.tsx b/src/contents/methods.tsx
index 81604f0..b2b7e75 100644
--- a/src/contents/methods.tsx
+++ b/src/contents/methods.tsx
@@ -23,7 +23,11 @@ export function Methods() {
<p>Patch clamp recording involves the use of a glass micropipette which is manufactured from a glass capillary through the use of a Micropipette Puller. The micropipette is then filled with an electrolyte solution, which is subsequently brought into contact with the cell membrane. By applying gentle suction, a high-resistance seal called giga seal is formed between the pipette tip and the membrane patch. This enables the measurement of ionic currents with minimal noise interference [3]. <strong>Whole-Cell Configuration</strong> records currents from the entire cell by rupturing the membrane patch, accessing the intracellular environment, and is useful for analysing overall ion channel activity and cellular responses. <strong>Single-Channel Recording</strong> measures currents through individual ion channels without rupturing the membrane, enabling high-resolution study of channel conductance, gating, and selectivity [2].</p>
<div className="figure-wrapper">
<figure>
- <iframe title="Bielefeld-CeBiTec: Patch Clamp Measurement (2024)" width="560" height="315" src="https://video.igem.org/videos/embed/0d948e57-5997-430a-a2df-815b71a2fc67?autoplay=1" frameBorder="0" allowFullScreen={true} sandbox="allow-same-origin allow-scripts allow-popups allow-forms"></iframe>
+ <div className="row align-items-center">
+ <div className="col">
+ <iframe title="Bielefeld-CeBiTec: Patch Clamp Measurement (2024)" width="560" height="315" src="https://video.igem.org/videos/embed/0d948e57-5997-430a-a2df-815b71a2fc67?autoplay=1" frameBorder="0" allowFullScreen={true} sandbox="allow-same-origin allow-scripts allow-popups allow-forms"></iframe>
+ </div>
+ </div>
<figcaption> <b>Figure 1. </b> Microscopic recording of micropipette sealing of a HEK293 cell. </figcaption>
</figure>
</div>
--
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