diff --git a/src/contents/methods.tsx b/src/contents/methods.tsx index db91407dc027371890f3db42200b8dd6b7bc4881..d8005c32366bd2c8b2f273060ae0fe560255d386 100644 --- a/src/contents/methods.tsx +++ b/src/contents/methods.tsx @@ -15,7 +15,7 @@ export function Methods() { <H4 text="Principles of the patch clamp technique"></H4> <p>Patch clamp recording involves the use of a glass micropipette which is manufactured from a glass capillary through the use of a Micropipette Puller. The micropipette is then filled with an electrolyte solution, which is subsequently brought into contact with the cell membrane. By applying gentle suction, a high-resistance seal called giga seal is formed between the pipette tip and the membrane patch. This enables the measurement of ionic currents with minimal noise interference [3]. Whole-Cell Configuration records currents from the entire cell by rupturing the membrane patch, accessing the intracellular environment, and is useful for analysing overall ion channel activity and cellular responses. Single-Channel Recording measures currents through individual ion channels without rupturing the membrane, enabling high-resolution study of channel conductance, gating, and selectivity [2].</p> //TODO: Video Einfügen - <p>The success of patch clamp experiments heavily depends on the composition of the solutions used. Typically, two main types of solutions are employed: The Pipette Solution in the micropipette mimics the intracellular environments, while the Bath Solution surrounds the cell and usually contains components that replicate the extracellular environment. Both solutions are meticulously designed to reflect the physiological conditions under which the cells operate, thereby ensuring that the measurements accurately reflect ion channel activity in a natural setting [2].</p><img src="https://static.igem.wiki/teams/5247/photos/for-wiki-texts/meth-patch-clamp/bild-meth-patch-clamp.png" text="Patch clamp setup"></img> + <p>The success of patch clamp experiments heavily depends on the composition of the solutions used. Typically, two main types of solutions are employed: The Pipette Solution in the micropipette mimics the intracellular environments, while the Bath Solution surrounds the cell and usually contains components that replicate the extracellular environment. Both solutions are meticulously designed to reflect the physiological conditions under which the cells operate, thereby ensuring that the measurements accurately reflect ion channel activity in a natural setting [2].</p> <figure> <img src="https://static.igem.wiki/teams/5247/photos/for-wiki-texts/meth-patch-clamp/bild-meth-patch-clamp.png" alt="Patch clamp setup"/> <figcaption>Patch clamp setup</figcaption>