diff --git a/src/contents/Human Practices/Further Engagement/SupMaterial.tsx b/src/contents/Human Practices/Further Engagement/SupMaterial.tsx index 4648a1d3c5237d0e0a345d3abcedd37b704bcfe8..41947fec972e5849f80e11cfac32a6587ae3be27 100644 --- a/src/contents/Human Practices/Further Engagement/SupMaterial.tsx +++ b/src/contents/Human Practices/Further Engagement/SupMaterial.tsx @@ -1,10 +1,18 @@ +import { DownloadLink } from "../../../components/Buttons"; import { Section } from "../../../components/sections"; export function HPSupplement(){ return( <Section title="Supplementary Material" id="Supplementary Material"> - child + <div className="row"> + <div className="col"> + <p>Click here to see our raw data of our surverys</p> + </div> + <div className="col"> + <DownloadLink url="https://static.igem.wiki/teams/5247/pdfs/raw-data-surveys.pdf" fileName="raw-data-surveys.pdf"></DownloadLink> + </div> + </div> </Section> ) } \ No newline at end of file diff --git a/src/contents/notebook.tsx b/src/contents/notebook.tsx index 5ed64e29370c0aeee5f8f08d77c0c624a363322a..a272bc16c6065987bb1aa78395441b692752e48e 100644 --- a/src/contents/notebook.tsx +++ b/src/contents/notebook.tsx @@ -36,7 +36,7 @@ export function Notebook() { </div> <div className='row'> <div className="col"> - <DownloadImageButton url="" fileName=""> + <DownloadImageButton url="https://static.igem.wiki/teams/5247/pdfs/lab-book-5-downstream-experiments.pdf" fileName="lab-book-5-downstream.webp"> <img src="https://static.igem.wiki/teams/5247/lab-journals/titelseite-lab-book-5-downstream.webp" style={{height: "75%", width: "auto"}}/> </DownloadImageButton> </div> diff --git a/src/contents/safety.tsx b/src/contents/safety.tsx index 0c4bae6c2789ceda92011652b144d6a3089d2c38..82493769209ba05c587276a90ee075573cd7925d 100644 --- a/src/contents/safety.tsx +++ b/src/contents/safety.tsx @@ -16,7 +16,7 @@ export const Safety: React.FC = () =>{ <> <Section title="Role in iGEM" id="Role"> <p> - As part of our project [PreCyse] to develop a prime-editing complex to correct the F508del mutation in cystic fibrosis, we place great emphasis on safety at all stages of research. Our final construct will be tested in primary cultures of epithelial cells obtained from nasal swabs, isolated from both patients and healthy individuals. [link primär Kulturen]. To guarantee safety and ensure the highest level of precision and reliability of our results, we have introduced a series of carefully planned checkpoints during the experiments. These milestones allow for continuous monitoring, timely adjustments and validation at each critical stage. This ensures that potential issues are identified and addressed immediately, minimizing risk and improving the overall quality of the experimental results. [link zu den Experimenten] + As part of our project <PreCyse/> to develop a prime-editing complex to correct the F508del mutation in cystic fibrosis, we place great emphasis on safety at all stages of research. Our final construct will be tested in primary cultures of epithelial cells obtained from nasal swabs, isolated from both patients and healthy individuals. [link primär Kulturen]. To guarantee safety and ensure the highest level of precision and reliability of our results, we have introduced a series of carefully planned checkpoints during the experiments. These milestones allow for continuous monitoring, timely adjustments and validation at each critical stage. This ensures that potential issues are identified and addressed immediately, minimizing risk and improving the overall quality of the experimental results. [link zu den Experimenten] </p> </Section> <Section title="Check-Ins" id="Check-Ins"> @@ -29,16 +29,16 @@ export const Safety: React.FC = () =>{ Before commencing laboratory work, all participants were required to attend a mandatory safety briefing. In compliance with German regulations, each team member's participation had to be confirmed with a personal signature. The briefing, conducted by Prof. Dr. Kristian Müller must be renewed annually in accordance with §12 ArbSchG. It covered the following areas: </p> <p> - General laboratory safety + - General laboratory safety </p> <p> - Regulations regarding hazardous and toxic substances + - Regulations regarding hazardous and toxic substances </p> <p> - Regulations concerning biological materials + - Regulations concerning biological materials </p> <p> - Regulations on genetic engineering. + - Regulations on genetic engineering. </p> <p> In addition to the general safety briefing, specific instructions for the safe operation of each device were provided. The Safety and Security Officer within the laboratory highlighted the potential hazards and necessary precautionary measures. We have focused on planning our laboratory activities to minimize risk for safer practices. This ensures not only the safe and proper use of equipment but also the generation of reliable data. To meet all safety requirements, additional safety protocols have been put in place for all targeted areas of the laboratory equipment. @@ -80,7 +80,7 @@ export const Safety: React.FC = () =>{ </p> <H4 text="Check-in for Cloning"></H4> <p> - For our cloning experiments and the development of our prime editing complexes, we have amplified various plasmids in <i>E. coli</i> K-12 strains (DH5α,10-Beta). When working with microbial strains such as <i>E. coli</i> K-12 strains,it's important to consider potential risks associated with their use, even though they are generally regarded as safe in laboratory settings. All experiments were performed under strict S1 conditions, following all relevant safety protocols. Below you will find an overview of the <i>E. coli</i> K-12 strains for our cloning experiments, submitted by us as a check-In and the specific safety measures: + For our cloning experiments and the development of our prime editing complexes, we have amplified various plasmids in <i>E. coli</i> K-12 strains (DH5α,10-Beta). When working with microbial strains such as <i>E. coli</i> K-12 strains, it's important to consider potential risks associated with their use, even though they are generally regarded as safe in laboratory settings. All experiments were performed under strict S1 conditions, following all relevant safety protocols. Below you will find an overview of the <i>E. coli</i> K-12 strains for our cloning experiments, submitted by us as a check-In and the specific safety measures: </p> <p> <strong><i>E. coli K-12</i> strains (DH5α, 10-Beta):</strong> Although these strains are non-pathogenic and have been modified to minimize the risk of spreading antibiotic resistance, there remains a low risk of horizontal gene transfer, where genetic material could be transferred to other microorganisms, potentially leading to the spread of resistance genes or other traits. If accidentally released into the environment, <i>E. coli</i> K-12 strains could potentially interact with native microbial communities. While they are typically outcompeted in natural environments, there's a remote possibility of ecological disruption, particularly in microenvironments where they could find a niche.While these strains are non-virulent, they still pose a minimal risk to humans, particularly immunocompromised individuals, through accidental ingestion or inhalation in a laboratory setting. @@ -113,7 +113,7 @@ export const Safety: React.FC = () =>{ <strong>Human nasal epithelial cells (hNECs):</strong> Human nasal epithelial cells (hNECs) were harvested using a nasal brush, a minimally invasive procedure, and cultured in air-liquid interface (ALI) cultures to model the airway epithelium. Human nasal epithelial cells (hNECs) were obtained using a nasal brush, a minimally invasive technique, and then cultured in air-liquid interface (ALI) cultures to model the airway epithelium. Using these primary cultures, derived from donors with airway diseases such as cystic fibrosis, we were able to simulate the in vivo conditions of such diseases. Due to the sensitive nature of these primary human cells, we performed all experiments with hNECs in our S2 laboratory, where increased safety precautions were taken. This included strict safety controls, safe handling of samples and proper disposal of materials after testing. In particular, the hNECs underwent HHH (Triple H: HIV, HCV and HBV) testing to ensure that no contamination occurred during sample collection or experimentation. These tests included sterility testing, viability assessments and contamination testing to ensure the safety and integrity of both the samples and the laboratory environment. After a negative HHH test, the primary cultures can be treated as S1. In addition, the nasal epithelial cells were handled with the utmost care during collection, ensuring that all procedures were performed under sterile conditions to avoid any risk of contaminationFor this purpose, the intensive examination of ethical questions was fundamental and a constant companion of our project. The numerous results from the interviews in the areas of: Ethics, storage and training in the handling of samples have been summarized in a guideline for patient consent for Germany and are intended to provide iGEM teams with the scope, critical examination and observance of iGEM rules, international and national guidelines.  </p> - <H4 text="Checkin for Delivery "></H4> + <H4 text="Check-in for Delivery "></H4> <p> Our finished construct is designed to be delivered into the lung via an inhaler using lipid nanoparticles (LNPs). To be more spezific a selective organ-targeting (SORT)- LNPs were developed to deliver mRNA specifically to the lung, with special measures taken to increase biocompatibility and safety. Since the LNP composition is very specific and also differs from other formulas, we submitted the LNP as a checkin: </p> diff --git a/src/data/hptimelinedata.tsx b/src/data/hptimelinedata.tsx index 6a4e6424860790dc49082dcc22cce509d7f9074f..de0ce75a61df57213e9fb7beafc8c3fb6dde3a49 100644 --- a/src/data/hptimelinedata.tsx +++ b/src/data/hptimelinedata.tsx @@ -1,6 +1,14 @@ import { QaBox } from "../components/Boxes"; import { TabScrollLink } from "../components/Link"; import { ScrollLinkWithChild } from "../components/ScrollLink"; +import { useNavigation } from "../utils"; + + +export function cellCultures () { + const {goToPageAndScroll} = useNavigation(); + + return (<a onClick={() => goToPageAndScroll('hek293-and-hek293t-cell-lines', '/materials-methods')}> linktext </a> ); +} export interface TimelineDatenpunkt { title?: string; /* Prof. , Dr., ... */ @@ -838,6 +846,7 @@ export const timelinedata: Array<TimelineDatenpunkt> = [ Neurophysiology working group at Bielefeld University, seemed to be an ideal interviewee. His knowledge and experience promised valuable insights and advice for conducting and optimizing our experiments. </p>], + pictureurl_aim: "https://static.igem.wiki/teams/5247/photos/for-wiki-texts/hp-patch-clamp/wischmeyer-interview.webp", insights: [<><p>Prof. Dr. Wischmeyer taught us about the workflow of the Patch-Clamp technique. He highlighted the need for specialized electrodes and glass pipettes that must form a smooth surface devoid of the extracellular matrix (ECM). Additionally, he pointed out that measuring CFTR conductivity with the Patch-Clamp technique @@ -852,7 +861,7 @@ export const timelinedata: Array<TimelineDatenpunkt> = [ the measurement of the membrane potential above and below a monolayer of confluent cells<ScrollLinkWithChild targetId="desc-3"><sup>3</sup></ScrollLinkWithChild>. Consequently, it enables precise measurement of conductivity dependent on CFTR expression. </p> </>], - implementation: [<> <p>We decided to use HEK293T cells lines from Mattijs Bulcaen from KU Leuven [Link] which do overexpress the + implementation: [<> <p>We decided to use HEK293T cells lines which do overexpress the correct CFTR and those which express CFTR with F508del for the Patch-Clamp measurements. To conduct the Patch-Clamp experiments, we contacted the Cellular Neurophysiology group to perform the necessary measurements. It was a pleasure to work together with Dr. Oliver Dräger[Link], who is working as a post-doc for @@ -862,8 +871,8 @@ export const timelinedata: Array<TimelineDatenpunkt> = [ Oliver Dräger, we gained valuable insights and optimized our approach to effectively investigate and measure the functionality of the CFTR ion channel, thereby determining the efficiency of our Prime Editing strategy. </p></>], - pictureurl_implementation: "https://static.igem.wiki/teams/5247/photos/for-wiki-texts/hp-patch-clamp/bild-interssierte-wissenschaftler-oho.jpeg", - pictureurl_aim: "https://static.igem.wiki/teams/5247/photos/for-wiki-texts/hp-patch-clamp/20240625-184032.jpg", + pictureurl_implementation: "https://static.igem.wiki/teams/5247/photos/for-wiki-texts/hp-patch-clamp/bild-patch-clamp-isi-oliver.webp", + pictureurl_interview: "https://static.igem.wiki/teams/5247/photos/for-wiki-texts/hp-patch-clamp/bild-interssierte-wissenschaftler-oho.webp", references: [ <ol> {/*<!-- Citation num 1--> */} @@ -908,7 +917,19 @@ export const timelinedata: Array<TimelineDatenpunkt> = [ </li> </ol> ], - summary: "" + interview: + <> + <QaBox q="Can you educate us about your academic career?" a="I did my doctorate 30 years ago at Bielefeld University and then worked at the Max Planck Institute in Göttingen a lot with the patch-clamp technique. Today, I’m head of the working group Cellular Neurophysiology of the medicine faculty of Bielefeld University." /> + <QaBox q="What new methods are currently available in electrophysiological research?" a="One of the latest methods is E-cis measurements. These make it possible to examine a monolayer of confluent cells and to measure the membrane potential both above and below. The change in conductivity can be analyzed for instance as a function of CFTR expression." /> + <QaBox q="How can we proceed with the investigation of CFTR in different cell cultures by patch-clamp?" a="You can study CFTR expression in HEK cells, which allows for a measurable change in chloride conductance. I am not sure whether we will be able to investigate CFTR sufficiently in epithelial cells which you want to collect from your CF patient friend and your team members. That is something we have to try out." /> + <QaBox q="How challenging is the measurement of CFTR conductance in epithelial cells?" a="CFTR in epithelial cells has very low conductivity in the femtoampere range. Therefore, extremely sensitive testing is necessary to obtain meaningful results." /> + <QaBox q="How challenging is the patch-clamp measurement of CFTR conductance in epithelial cells?" a="The project could take at least one year, even for experienced researchers." /> + <QaBox q="What technical challenges do we face in implementing the patch-clamp measurements?" a="One of the biggest challenges is measuring the current across the entire cell, as we do not want to carry out single-channel measurements, but rather record the current across cells with a strongly expressing vector carrying the gene for the ion channel." /> + <QaBox q="What requirements must be met for cultivation and transfection before the patch-clamp measurement?" a="You have to cultivate the cells on poly-lysine and laminin and use round coverslips of 10 mm diameter to prepare them for measurement. For identification of positive transfectants, we use GFP co-transfected cells in our working group, you should think of something like that as well. A transfection rate of 10 % is sufficient to gain enough cells for the measurement. You can think of optimizing your transfection by using Lipofectamine2000, which works well for our working group." /> + <QaBox q="Who could help us with the patch-clamp measurements?" a="The patch-clamp devices are heavily utilized in our working group, so you probably cannot perform measurements on your own. However, postdocs could support you for some measurements. Dr. Oliver Dräger is available as a contact person of my working group." /> + + </>, + summary: "n summary, through the interview with Prof. Dr. Wischmeyer and the collaboration with his employee Dr. Oliver Dräger, we gained valuable insights and optimized our approach to effectively investigate and measure the functionality of the CFTR ion channel, thereby determining the efficiency of our prime editing strategy." }, { title: "Prof. Dr.",