diff --git a/src/App/App.css b/src/App/App.css
index c7fc6c88c5baf88f499588056e8e8ab0d5d318e8..12907f07d3f754fc3f9493c14dc911e0cd217ef0 100644
--- a/src/App/App.css
+++ b/src/App/App.css
@@ -3478,9 +3478,16 @@ svg a:hover text{
.hp-timeline-img{
margin-top: 0 !important;
height: 100%; /* Das Bild wird in der Höhe an den Container angepasst */
- max-height: 100%;
width: 100%; /* Bild füllt den Image-Container aus */
- object-fit: cover; /* Optional: Skaliert das Bild so, dass es den Container proportional füllt */
+ object-fit: contain; /* Optional: Skaliert das Bild so, dass es den Container proportional füllt */
+ margin: auto;
+}
+
+.hti-right{
+ padding-right: var(--simple-padding) !important;
+}
+.hti-left{
+ padding-left: var(--simple-padding) !important;
}
.hti-box{
diff --git a/src/App/Timelines.css b/src/App/Timelines.css
index cfcd4b11d11021362271a53aa6692cf4183f6a32..fe6400a9873dd7d082955dbe3a1b99dc8d2814f4 100644
--- a/src/App/Timelines.css
+++ b/src/App/Timelines.css
@@ -7,7 +7,7 @@
white-space: nowrap;
min-height: 700px;
width: 75vw;
- overflow-x: hidden !important;
+ overflow-x: auto !important;
max-width: inherit !important;
overflow-y: hidden !important;
width: 100%;
diff --git a/src/contents/description.tsx b/src/contents/description.tsx
index 5d8eb830c58376c971a192de0e24c3d70fa37e60..d597eeaa769be89837d1a8b1667fee8b888fc2f6 100644
--- a/src/contents/description.tsx
+++ b/src/contents/description.tsx
@@ -342,8 +342,8 @@ export function Description() {
</Section>
<Section title="Our Achievement" id="Our Achievement">
- <p>We have successfully demonstrated a <b>proof of concept</b> for our gene therapy approach targeting Cystic Fibrosis. In initial experiments, HEK cells carrying a 3-base deletion analogous to the <i>F508del</i> mutation were transfected with our prime editing complex. The results met our expectations, confirming the viability of our approach for precise gene correction. Based on these findings, we optimized the prime editing complex, leading to the creation of <i>PrimeGuide</i>, a more compact and efficient editing tool. </p>
- <p>Central to our <b>delivery system</b> is <b>AirBuddy</b>, a lung-specific lipid nanoparticle designed to stabilize and protect the prime editing complex during transport to lung epithelial cells. <i>AirBuddy</i> ensures that the protein complex is delivered specifically to lung cells, enhancing the efficiency of the gene-editing process. By modifying the lipid nanoparticle with protective features, we achieved increased stability, ensuring effective delivery to the target cells. </p>
+ <p>We have successfully demonstrated a <b>proof of concept</b> for our gene therapy approach targeting Cystic Fibrosis. In initial experiments, HEK cells carrying a 3-base deletion analogous to the <i>F508del</i> mutation were transfected with our prime editing complex. The results met our expectations, confirming the viability of our approach for precise gene correction. Based on these findings, we optimized the prime editing complex, leading to the creation of <b>PrimeGuide</b>, a more compact and efficient editing tool. </p>
+ <p>Central to our <b>delivery system</b> is <b>AirBuddy</b>, a lung-specific lipid nanoparticle designed to stabilize and protect the prime editing complex during transport to lung epithelial cells. <b>AirBuddy</b> ensures that the protein complex is delivered specifically to lung cells, enhancing the efficiency of the gene-editing process. By modifying the lipid nanoparticle with protective features, we achieved increased stability, ensuring effective delivery to the target cells. </p>
<p>We further optimized the prime editing fusion protein, <b>PrimeGuide</b>, to streamline its components, resulting in a smaller and more efficient prime editing complex. This improvement significantly enhances the precision of the gene editing process, reducing off-target effects and increasing the overall success of mutation correction. </p>
<p>In subsequent experiments, <b>HEK and lung (CFBE41o-)cells</b> carrying the CFTR <i>F508del</i> mutation were successfully <b>transfected</b> with the optimized prime editing complex. Our results indicated successful correction of the mutation, confirming the potential of our approach for treating Cystic Fibrosis. </p>
<p>Additionally, we explored <b>downstream applications</b>. Primary cell cultures were treated with lipid nanoparticles to introduce a reporter RNA. We also established 2D cultures transfected with YFP, a sodium-sensitive reporter protein, to assess ion channel functionality. Finally, in CFTR-deficient organoids, our system facilitated repair of the CFTR channel, evidenced by an increase in organoid volume upon treatment. This suggests successful functional restoration of CFTR activity. </p>
diff --git a/src/data/hptimelinedata.tsx b/src/data/hptimelinedata.tsx
index 9747c5a861077519db31af41c36d93afeaa5f232..2b63eabed756165431afb259f4ffb96d348dea3a 100644
--- a/src/data/hptimelinedata.tsx
+++ b/src/data/hptimelinedata.tsx
@@ -162,11 +162,11 @@ export const timelinedata: Array<TimelineDatenpunkt> = [
type: "meta",
summary: [<p>
Building a successful interdisciplinary team, like the one involved in this iGEM project, requires careful coordination and a shared vision for addressing complex scientific challenges. This team is an excellent example of how bringing together individuals from various academic backgrounds fosters innovation and facilitates problem-solving in the fields of synthetic biology and biotechnology.</p>,
- <p><strong>The team members come from diverse fields of study, including Molecular Biotechnology, Bioinformatics, Molecular Cell Biology, Genome-Based System Biology, Interdisciplinary Biomedicine, and even Media Production.</strong></p>, <p>This variety of expertise allows them to approach problems from multiple perspectives, ensuring that the project is well-rounded and capable of addressing both
+ <p><b>The team members come from diverse fields of study, including Molecular Biotechnology, Bioinformatics, Molecular Cell Biology, Genome-Based System Biology, Interdisciplinary Biomedicine, and even Media Production.</b></p>, <p>This variety of expertise allows them to approach problems from multiple perspectives, ensuring that the project is well-rounded and capable of addressing both
experimental and computational challenges. For instance, while the biotechnologists and molecular biologists focus on designing and conducting lab experiments, the bioinformaticians contribute crucial insights for data analysis and genome sequencing. The inclusion of a media production expert adds another layer to the team’s capabilities, ensuring that science communication and
project documentation are handled professionally, which is particularly important for presenting their work at conferences and competitions.
The multidisciplinary structure of this team evolved naturally as they identified the project’s needs and recruited members who could fill specific roles. Over time, this multidisciplinary structure has continued to develop as the team’s project evolved. Roles became more defined as team members took on specific responsibilities, whether it was refining prime editing systems or crafting
- communication strategies for iGEM. The range of experience across the team, from younger students like Vincent Carl Stöckl in the early stages of their academic careers to more advanced students in later semesters, allows for a blend of fresh perspectives and seasoned expertise. This balance helps ensure both the immediate success of the project and the continuity of its progress in the future.
+ communication strategies for iGEM. The range of experience across the team, from younger students like Malte Lenger in the early stages of their academic careers to more advanced students in later semesters, allows for a blend of fresh perspectives and seasoned expertise. This balance helps ensure both the immediate success of the project and the continuity of its progress in the future.
In summary, this interdisciplinary iGEM team demonstrates how a diverse group of individuals with specialized skills can come together to tackle a complex biological project. By combining their expertise in molecular biology, bioinformatics, cell biology, and media production, the team is able to execute their project holistically, ensuring that each aspect, from experimental design to public outreach, is handled with precision and creativity.</p>],
months: "February"
},
@@ -184,11 +184,11 @@ export const timelinedata: Array<TimelineDatenpunkt> = [
quoteVorname: "Vera",
quote: "Firstly, we discussed various project ideas, including the use of magnetic microswimmers for targeted medical applications, gene editing approaches for fibrosis, treatments for muscular dystrophy and combating cyanobacteria with algae.",
type: "meta",
- summary: [<p>During our initial discussions about project ideas, the team explored several innovative concepts before honing in on <strong> fibrosis</strong>. Each project presented unique scientific challenges and potential impacts.</p>,
- <p>One idea involved the development of <strong>magnetic microswimmers</strong> designed for targeted cancer therapy, particularly for ovarian cancer. The proposal aimed to overcome the limitations of traditional treatments, such as radiotherapy and cisplatin, which often damage healthy cells and result in numerous side effects. The microswimmers would be biodegradable algae-based vehicles, magnetically guided to deliver drugs directly to cancerous cells while minimizing damage to surrounding tissue. This approach offered a novel, non-invasive targeting mechanism, leveraging the potential of magnetic fields to direct the swimmers precisely to the affected areas.</p>,
- <p>Another compelling project was focused on <strong>Duchenne muscular dystrophy (DMD)</strong>. This genetic disorder leads to muscle degeneration as muscle tissue is gradually replaced by fibrotic tissue. The team proposed several treatment approaches, including exon skipping and gene therapy using viral vectors. Key strategies included enhancing Cas protein efficiency through engineering, using liposomal vectors for gene delivery, and combining CRISPR technology with integrase fusion proteins. The team also discussed previous iGEM projects that had explored similar methodologies, emphasizing the need for specificity and efficacy in delivering therapeutic genes.</p>,
- <p>We also considered a project aimed at preventing <strong>cyanobacteria blooms</strong> using peptides or proteins derived from algae. Cyanobacteria can produce harmful toxins that degrade water quality and threaten ecosystems. The proposed approach focused on utilizing natural algae to develop mechanisms that inhibit the growth of harmful cyanobacteria without disrupting the ecosystem. This project emphasized conservation and biodiversity while addressing a significant environmental issue linked to the climate crisis.</p>,
- <p>Throughout our discussions, we recognized that while each project had merit, the <strong> fibrosis</strong> initiative offered the most promise for a targeted approach to a well-defined problem. CF, affecting a substantial number of individuals, provided a clear opportunity for meaningful impact through gene therapy. The insights gained from our examination of other projects informed our approach to CF, allowing us to integrate knowledge from diverse fields and methodologies, ultimately shaping a comprehensive and innovative project direction.</p>
+ summary: [<p>During our initial discussions about project ideas, the team explored several innovative concepts before honing in on <b> Cystic Fibrosis</b>. Each project presented unique scientific challenges and potential impacts.</p>,
+ <p>One idea involved the development of <b>magnetic microswimmers</b> designed for targeted cancer therapy, particularly for ovarian cancer. The proposal aimed to overcome the limitations of traditional treatments, such as radiotherapy and cisplatin, which often damage healthy cells and result in numerous side effects. The microswimmers would be biodegradable algae-based vehicles, magnetically guided to deliver drugs directly to cancerous cells while minimizing damage to surrounding tissue. This approach offered a novel, non-invasive targeting mechanism, leveraging the potential of magnetic fields to direct the swimmers precisely to the affected areas.</p>,
+ <p>Another compelling project was focused on <b>duchenne muscular dystrophy (DMD)</b>. This genetic disorder leads to muscle degeneration as muscle tissue is gradually replaced by fibrotic tissue. The team proposed several treatment approaches, including exon skipping and gene therapy using viral vectors. Key strategies included enhancing Cas protein efficiency through engineering, using liposomal vectors for gene delivery, and combining CRISPR technology with integrase fusion proteins. The team also discussed previous iGEM projects that had explored similar methodologies, emphasizing the need for specificity and efficacy in delivering therapeutic genes.</p>,
+ <p>We also considered a project aimed at preventing <b>cyanobacteria blooms</b> using peptides or proteins derived from algae. Cyanobacteria can produce harmful toxins that degrade water quality and threaten ecosystems. The proposed approach focused on utilizing natural algae to develop mechanisms that inhibit the growth of harmful cyanobacteria without disrupting the ecosystem. This project emphasized conservation and biodiversity while addressing a significant environmental issue linked to the climate crisis.</p>,
+ <p>Throughout our discussions, we recognized that while each project had merit, the <b> Cystic Fibrosis</b> initiative offered the most promise for a targeted approach to a well-defined problem. CF, affecting a substantial number of individuals, provided a clear opportunity for meaningful impact through gene therapy. The insights gained from our examination of other projects informed our approach to CF, allowing us to integrate knowledge from diverse fields and methodologies, ultimately shaping a comprehensive and innovative project direction.</p>
],
months: "March"
},
@@ -310,9 +310,9 @@ export const timelinedata: Array<TimelineDatenpunkt> = [
interviewtabid: "hannovermesse",
cardtext: "",
quote: "Visiting Hannover Messe was inspiring! We connected with industry leaders and discovered exciting innovations in synthetic biology that could shape our future projects.",
- quoteNachname: "Stöckl, Teammember",
- quoteVorname: "Vincent",
- summary: "Our team members Liliana Sanfilippo and Vincent Stöckl had a productive visit to the Hannover Messe, focusing on synthetic biology and research advancements. They explored innovative technologies and networked with potential sponsors, gaining valuable insights to help enhance our project and drive future developments. The event was a great opportunity to connect with industry leaders and learn about cutting-edge solutions in the field.",
+ quoteNachname: "Sanfilipo, Teammember",
+ quoteVorname: "Liliana",
+ summary: "Our team members had a productive visit to the Hannover Messe, focusing on synthetic biology and research advancements. They explored innovative technologies and networked with potential sponsors, gaining valuable insights to help enhance our project and drive future developments. The event was a great opportunity to connect with industry leaders and learn about cutting-edge solutions in the field.",
months: "April",
pictureurl_implementation: "https://static.igem.wiki/teams/5247/photos/hp/hannover.webp",
},
@@ -360,19 +360,19 @@ export const timelinedata: Array<TimelineDatenpunkt> = [
Through connections with the University Hospital Münster and our local hospital, we aimed to gain a comprehensive overview of the clinical applications of gene therapy and the current research in fibrosis. These consultations with specialists allowed us to acquire valuable insights into different therapeutic options and laid the groundwork for our own exploration of potential strategies, particularly in the area of prime editing as a promising treatment avenue.</p>,
<ul>
<li>
- <strong>Team Formation & Research:</strong> Chose gene therapy for fibrosis and explored mechanisms and delivery strategies.
+ <b>Team Formation & Research:</b> Chose gene therapy for fibrosis and explored mechanisms and delivery strategies.
</li>
<li>
- <strong>Expert Engagement:</strong> Consulted with specialists to refine approach, focusing on prime editing.
+ <b>Expert Engagement:</b> Consulted with specialists to refine approach, focusing on prime editing.
</li>
<li>
- <strong>Medical Collaboration:</strong> Gained clinical insights through partnerships with hospitals.
+ <b>Medical Collaboration:</b> Gained clinical insights through partnerships with hospitals.
</li>
<li>
- <strong>Project Development:</strong> Developed detailed plans for gene therapy, incorporating expert feedback.
+ <b>Project Development:</b> Developed detailed plans for gene therapy, incorporating expert feedback.
</li>
<li>
- <strong>Testing & Future Application:</strong> Plan to test strategies and prepare for potential clinical trials.
+ <b>Testing & Future Application:</b> Plan to test strategies and prepare for potential clinical trials.
</li>
</ul>
@@ -507,13 +507,13 @@ export const timelinedata: Array<TimelineDatenpunkt> = [
At this stage, we are eager to expand our perspectives by seeking input from industry and business professionals, while also striving to increase our local impact. To evaluate this impact, we plan to develop a survey aimed at understanding the interest in gene therapy and the community’s knowledge of fibrosis within our local area. This will help us gauge awareness and ensure our project addresses both scientific and societal needs effectively.</p>,
<ul>
<li>
- <strong>Expert Feedback Integration:</strong> Refined the project focus on optimizing Prime Editing strategies and lung-specific gene delivery based on clinical and academic insights.
+ <b>Expert Feedback Integration:</b> Refined the project focus on optimizing Prime Editing strategies and lung-specific gene delivery based on clinical and academic insights.
</li>
<li>
- <strong>Focus on Lung-Specific Correction:</strong> Shifted toward lung-specific CFTR gene correction for fibrosis treatment.
+ <b>Focus on Lung-Specific Correction:</b> Shifted toward lung-specific CFTR gene correction for fibrosis treatment.
</li>
<li>
- <strong>Community Engagement Plan:</strong> Initiated plans for a local survey to assess awareness of fibrosis and interest in gene therapy, aiming to increase local impact.
+ <b>Community Engagement Plan:</b> Initiated plans for a local survey to assess awareness of fibrosis and interest in gene therapy, aiming to increase local impact.
</li>
</ul>
],
@@ -599,19 +599,19 @@ export const timelinedata: Array<TimelineDatenpunkt> = [
<ul>
<li>
- <strong>Commitment to Human-Centric Approach:</strong> Early in the project, the decision to follow a human-centric approach ensured that societal impact, ethical considerations, and the needs of patients would be central to all scientific developments.
+ <b>Commitment to Human-Centric Approach:</b> Early in the project, the decision to follow a human-centric approach ensured that societal impact, ethical considerations, and the needs of patients would be central to all scientific developments.
</li>
<li>
- <strong>Selection of Special Prizes (Biosafety and Security, Best Integrated Human Practices):</strong> Prioritizing biosafety, security, and human practices from the start highlighted the project’s focus on safety, ethical responsibility, and community engagement, aligning with broader human-centric goals.
+ <b>Selection of Special Prizes (Biosafety and Security, Best Integrated Human Practices):</b> Prioritizing biosafety, security, and human practices from the start highlighted the project’s focus on safety, ethical responsibility, and community engagement, aligning with broader human-centric goals.
</li>
<li>
- <strong>Collaboration with Stemcell Technologies:</strong> Partnering with industry leaders provided technical expertise that allowed the team to deepen their understanding of human cell cultivation and gene editing, ensuring that the project’s technological developments were informed by real-world applications.
+ <b>Collaboration with Stemcell Technologies:</b> Partnering with industry leaders provided technical expertise that allowed the team to deepen their understanding of human cell cultivation and gene editing, ensuring that the project’s technological developments were informed by real-world applications.
</li>
<li>
- <strong>Expansion of Biosafety Standards:</strong> Extending biosafety protocols to mimic preclinical trial conditions reinforced the commitment to safe, ethical research practices and laid the groundwork for clinical relevance, demonstrating responsibility toward future patients.
+ <b>Expansion of Biosafety Standards:</b> Extending biosafety protocols to mimic preclinical trial conditions reinforced the commitment to safe, ethical research practices and laid the groundwork for clinical relevance, demonstrating responsibility toward future patients.
</li>
<li>
- <strong>Local and International Community Engagement:</strong> Efforts to raise awareness at both local and global levels ensured that the project was not only scientifically sound but also socially responsible, with a focus on educating and involving the public in the conversation around fibrosis and gene therapy.
+ <b>Local and International Community Engagement:</b> Efforts to raise awareness at both local and global levels ensured that the project was not only scientifically sound but also socially responsible, with a focus on educating and involving the public in the conversation around fibrosis and gene therapy.
</li>
</ul>
],
@@ -762,13 +762,13 @@ export const timelinedata: Array<TimelineDatenpunkt> = [
<ul>
<li>
- <strong>Survey Insights:</strong> Many participants were motivated to try gene therapies but lacked knowledge about them and fibrosis, highlighting the need for better public education.
+ <b>Survey Insights:</b> Many participants were motivated to try gene therapies but lacked knowledge about them and fibrosis, highlighting the need for better public education.
</li>
<li>
- <strong>Awareness Campaign:</strong> Partnered with Mukoviszidose e.V. Deutschland to support the Muko Move campaign, raising awareness about fibrosis in the local community.
+ <b>Awareness Campaign:</b> Partnered with Mukoviszidose e.V. Deutschland to support the Muko Move campaign, raising awareness about fibrosis in the local community.
</li>
<li>
- <strong>Scientific Advancements:</strong> Improved the efficiency of Prime Editing by incorporating a novel RNA structural element, with further efforts to optimize the Prime Editing protein complex for broader applicability beyond fibrosis.
+ <b>Scientific Advancements:</b> Improved the efficiency of Prime Editing by incorporating a novel RNA structural element, with further efforts to optimize the Prime Editing protein complex for broader applicability beyond fibrosis.
</li>
</ul>],
months: "june"
@@ -969,19 +969,19 @@ export const timelinedata: Array<TimelineDatenpunkt> = [
implementation: "The most important thing was that both Katrin and the parents agreed that the children were able to inhale at an early age and that there were generally no physical problems with inhalation in general. This reinforced our decision to work towards delivery by inhalation. It was very interesting to see the different ways children deal with their exercises and hear about the progress they made. ",
text: [<ol>
<li>
- <strong>Robin (>10)</strong>
+ <b>Robin (>10)</b>
<p>Robin will soon start 4th grade and takes modulators. Since taking them, many problems have subsided. No regular pneumonia with long hospital stays and the mucus comes out easier. Nevertheless, Robin still goes to physiotherapy regularly to do manual breathing therapy to get the mucus out. Katrin tells us how the mucus changes color the longer it stays in the lungs. The new mucus is white, and the older mucus gets yellow first and then gets darker with time until it reaches a black color. Nowadays, Robin rarely has dark mucus or clumps, but we can still hear the rustling as Katrin starts the autogenous drainage (Autogene Drainage) by pressing on Robin's chest. The goal is to get out the mucus deep in the lungs. To do that, Robin must repeat the routine – breathing in deeply, holding, breathing out – multiple times and then cough and spit the mucus out. Sometimes it works, but other times the mucus does not come out easily. While according to Katrin the autogenous drainage is the gold standard, they do other useful exercises, too. For example, pressing the Vojta points (which the children call “the magic pointsâ€) on the chest to activate a deep breathing reflex and get air into parts of the lungs that may not have been used previously. Or physical activity such as climbing a few steps on a climbing ladder and hanging on it to stretch the thorax muscles.</p>
</li>
<li>
- <strong>Sam (<10) & Alex (<10)</strong>
+ <b>Sam (<10) & Alex (<10)</b>
<p>Sam and Alex are siblings and do not have CF but another affliction that causes a persistent cough. They come together with a parent twice a week and do hanging exercises from the ceiling, nasal showers with needleless syringes, and the “magic points.†Katrin also checks their lungs for mucus in a similar manner to autogenous drainage. We, too, tried to do the nasal shower, and being a grown-up really does not guarantee being able to do that properly! This highlighted that the children know all their exercises by heart at a young age. On request, their parent told us that the physiotherapy made a big difference for both of them.</p>
</li>
<li>
- <strong>Toni (<5)</strong>
+ <b>Toni (<5)</b>
<p>Toni has a light version of CF and has been doing physiotherapy with Katrin since shortly after birth. In contrast to most children we met or talked about, Toni refuses medication. Modulators are a possibility, but them and 'everything stinky' is a no-go, even though inhaling would be very beneficial due to the mucus buildup. Most exercises result in crying and screaming, which is very exhausting for the child. Due to the light nature of Toni's variant, they are not in danger, but a permanent therapy would be very beneficial.</p>
</li>
<li>
- <strong>Chrissi (>10)</strong>
+ <b>Chrissi (>10)</b>
<p>Chrissi takes modulators and will soon take a trip to a water park with some friends. Katrin teaches us that when the children do not breathe out properly, air stays in the lungs and causes hyperinflation – with which it is actually harder to float in water! After the manual drainage, Katrin gets all of us glasses with water and dish soap and straws. Blowing bubbles is a playful way to train how to properly breathe out by either trying to blow bubbles as long as possible or trying to make an existing bubble as big as possible!</p>
</li>
</ol>,],
@@ -1143,19 +1143,19 @@ export const timelinedata: Array<TimelineDatenpunkt> = [
</p>,
<ul>
<li>
- <strong>Overcoming Experimental Challenges:</strong> Improved delivery strategies based on stakeholder feedback, successfully using lipid nanoparticles to transfect HEK and CFTR-specific cells.
+ <b>Overcoming Experimental Challenges:</b> Improved delivery strategies based on stakeholder feedback, successfully using lipid nanoparticles to transfect HEK and CFTR-specific cells.
</li>
<li>
- <strong>Proof of Concept:</strong> Successfully replicated the Prime Editing proof of concept established by David Liu et al. (2019), gaining valuable insights into system functionality.
+ <b>Proof of Concept:</b> Successfully replicated the Prime Editing proof of concept established by David Liu et al. (2019), gaining valuable insights into system functionality.
</li>
<li>
- <strong>Development of Fluorescence-Based Efficiency Measurement:</strong> Created a highly selective fluorescence-based system to accurately measure the efficiency of Prime Editing constructs.
+ <b>Development of Fluorescence-Based Efficiency Measurement:</b> Created a highly selective fluorescence-based system to accurately measure the efficiency of Prime Editing constructs.
</li>
<li>
- <strong>Industry Collaboration:</strong> Established connections with industry members for sponsorships, collaboration, and feedback on broader challenges such as insurance issues tied to fibrosis treatment.
+ <b>Industry Collaboration:</b> Established connections with industry members for sponsorships, collaboration, and feedback on broader challenges such as insurance issues tied to fibrosis treatment.
</li>
<li>
- <strong>Optimization of LNPs for Lung-Specific Delivery:</strong> Began optimizing lipid nanoparticles for lung-specific delivery, while preparing for enzyme engineering to enhance the Prime Editing system.
+ <b>Optimization of LNPs for Lung-Specific Delivery:</b> Began optimizing lipid nanoparticles for lung-specific delivery, while preparing for enzyme engineering to enhance the Prime Editing system.
</li>
</ul>
@@ -1219,13 +1219,13 @@ export const timelinedata: Array<TimelineDatenpunkt> = [
including offering discounts in exchange for recognition in publications.
The interaction with Corden Pharma provided several key insights:</p>,
<ul>
- <li><strong>Spray Drying Feasibility:</strong> Corden Pharma hasn’t explored spray drying extensively; consultation with specialists is recommended for assessing feasibility and lipid stability.</li>
- <li><strong>LNP Stability:</strong> Stability of LNPs, particularly for inhalation therapies, needs empirical testing, considering shear forces and the potential use of cryoprotectants or temperature control.</li>
- <li><strong>Lipid Selection in Kits:</strong> Starter kits use well-researched lipid combinations, tested for stability, encapsulation efficiency, and potency. They provide materials for multiple experimental batches.</li>
- <li><strong>Lipid Modifications:</strong> Exploring alternative lipids (e.g., cholesterol derivatives) could enhance stability and cellular uptake, tailored to project needs.</li>
- <li><strong>Antibody Incorporation:</strong> Antibodies can be incorporated into LNPs during preparation or afterward, depending on targeting requirements.</li>
- <li><strong>Intellectual Property:</strong> IP considerations are crucial when selecting lipids for LNP formulations, as many lipids are patented.</li>
- <li><strong>Collaboration Opportunities:</strong> Corden Pharma is open to offering discounts or forming partnerships, with recognition in publications or acknowledgments.</li>
+ <li><b>Spray Drying Feasibility:</b> Corden Pharma hasn’t explored spray drying extensively; consultation with specialists is recommended for assessing feasibility and lipid stability.</li>
+ <li><b>LNP Stability:</b> Stability of LNPs, particularly for inhalation therapies, needs empirical testing, considering shear forces and the potential use of cryoprotectants or temperature control.</li>
+ <li><b>Lipid Selection in Kits:</b> Starter kits use well-researched lipid combinations, tested for stability, encapsulation efficiency, and potency. They provide materials for multiple experimental batches.</li>
+ <li><b>Lipid Modifications:</b> Exploring alternative lipids (e.g., cholesterol derivatives) could enhance stability and cellular uptake, tailored to project needs.</li>
+ <li><b>Antibody Incorporation:</b> Antibodies can be incorporated into LNPs during preparation or afterward, depending on targeting requirements.</li>
+ <li><b>Intellectual Property:</b> IP considerations are crucial when selecting lipids for LNP formulations, as many lipids are patented.</li>
+ <li><b>Collaboration Opportunities:</b> Corden Pharma is open to offering discounts or forming partnerships, with recognition in publications or acknowledgments.</li>
</ul>
],
implementation: [<p>The insights from Corden Pharma had a major impact on our project, especially in selecting lipids critical for LNP stability and optimizing gene therapy applications. Initially, we used the Cayman kit, but it was suboptimal for delivering our Primeguide. After receiving feedback, we switched to Corden Pharma’s kit #2, which includes advanced lipid components like cationic lipids that improve cellular uptake and enhance LNP stability. This shift has significantly boosted the efficiency and robustness of our formulations.
@@ -1487,19 +1487,19 @@ export const timelinedata: Array<TimelineDatenpunkt> = [
</p>,
<ul>
<li>
- <strong>Enzyme Engineering Strategy Development:</strong> Developed an enzyme engineering strategy and identified potential nickase candidates in collaboration with Prof. Dr. Stefan Hammer.
+ <b>Enzyme Engineering Strategy Development:</b> Developed an enzyme engineering strategy and identified potential nickase candidates in collaboration with Prof. Dr. Stefan Hammer.
</li>
<li>
- <strong>Implementation of Primary Cell Cultures:</strong> Successfully implemented the use of primary cell cultures with the guidance of the Safety and Security Committee, including the creation of patient consent forms, BSL2 human biomaterial handling guidelines, and hygiene concepts.
+ <b>Implementation of Primary Cell Cultures:</b> Successfully implemented the use of primary cell cultures with the guidance of the Safety and Security Committee, including the creation of patient consent forms, BSL2 human biomaterial handling guidelines, and hygiene concepts.
</li>
<li>
- <strong>Ethics Committee Collaboration:</strong> Worked with the head of the Ethics Committee at Bielefeld University to ensure compliance with ethical standards, opening new opportunities for advancing research.
+ <b>Ethics Committee Collaboration:</b> Worked with the head of the Ethics Committee at Bielefeld University to ensure compliance with ethical standards, opening new opportunities for advancing research.
</li>
<li>
- <strong>Lipid Nanoparticle (LNP) Characterization:</strong> Completed initial characterization of LNPs and collaborated with other iGEM 2024 teams to innovate in LNP design.
+ <b>Lipid Nanoparticle (LNP) Characterization:</b> Completed initial characterization of LNPs and collaborated with other iGEM 2024 teams to innovate in LNP design.
</li>
<li>
- <strong>Wiki Development Focus:</strong> Shifted focus towards developing the project wiki to document and share progress with the iGEM community.
+ <b>Wiki Development Focus:</b> Shifted focus towards developing the project wiki to document and share progress with the iGEM community.
</li>
</ul>
],
@@ -1606,12 +1606,12 @@ export const timelinedata: Array<TimelineDatenpunkt> = [
implementation:[<p>We directly applied Katharina’s insights to improve our nanoparticle design and testing methods. Her recommendations on using nano-capsules and OptiMEM as the transfection medium helped refine our experimental approach. She emphasized using a medium with fewer additives, like OptiMEM, and suggested removing antibiotics 24 hours before transfection to avoid interference, ensuring more controlled and effective conditions. We also explored new stability testing ideas, including nasal spray solutions and dry powder formulations.
We incorporated her insights as follows: </p>,
<ul>
- <li><strong>Nano-Capsules Focus:</strong> Based on Katharina’s advice, we prioritized nano-capsules for their enhanced stability over nano-complexes.</li>
- <li><strong>Chitosan for Stability:</strong> We're exploring chitosan to improve mRNA delivery system stability due to its positive charge, which binds mRNA to the nanoparticle surface.</li>
- <li><strong>OptiMEM for Transfection:</strong> OptiMEM is now our chosen transfection medium, with the suggestion to remove antibiotics 24 hours prior.</li>
- <li><strong>MTT Test for Cytotoxicity:</strong> We adopted the MTT test for cytotoxicity due to its simplicity and reliability.</li>
- <li><strong>Nasal Spray and Dry Powder Testing:</strong> We are considering testing nanoparticle stability using nasal spray solutions and exploring dry powder formulations.</li>
- <li><strong>Capsaicin Exclusion:</strong> As capsaicin did not significantly impact transfection efficiency in Katharina’s research, we decided not to include it in our project.</li>
+ <li><b>Nano-Capsules Focus:</b> Based on Katharina’s advice, we prioritized nano-capsules for their enhanced stability over nano-complexes.</li>
+ <li><b>Chitosan for Stability:</b> We're exploring chitosan to improve mRNA delivery system stability due to its positive charge, which binds mRNA to the nanoparticle surface.</li>
+ <li><b>OptiMEM for Transfection:</b> OptiMEM is now our chosen transfection medium, with the suggestion to remove antibiotics 24 hours prior.</li>
+ <li><b>MTT Test for Cytotoxicity:</b> We adopted the MTT test for cytotoxicity due to its simplicity and reliability.</li>
+ <li><b>Nasal Spray and Dry Powder Testing:</b> We are considering testing nanoparticle stability using nasal spray solutions and exploring dry powder formulations.</li>
+ <li><b>Capsaicin Exclusion:</b> As capsaicin did not significantly impact transfection efficiency in Katharina’s research, we decided not to include it in our project.</li>
</ul>
] ,
interview:<>
@@ -1704,16 +1704,16 @@ export const timelinedata: Array<TimelineDatenpunkt> = [
<ul>
<li>
- <strong>Community Engagement:</strong> Connected with community organizations and advocacy groups to understand the societal implications of our research and garner support.
+ <b>Community Engagement:</b> Connected with community organizations and advocacy groups to understand the societal implications of our research and garner support.
</li>
<li>
- <strong>Social Media Outreach:</strong> Utilized platforms like LinkedIn to share progress and findings, promoting awareness of fibrosis and gene therapy.
+ <b>Social Media Outreach:</b> Utilized platforms like LinkedIn to share progress and findings, promoting awareness of fibrosis and gene therapy.
</li>
<li>
- <strong>Collaborative Discussions:</strong> Engaged in discussions with peers, faculty, and industry professionals, exchanging insights and refining methodologies.
+ <b>Collaborative Discussions:</b> Engaged in discussions with peers, faculty, and industry professionals, exchanging insights and refining methodologies.
</li>
<li>
- <strong>Broader Perspectives:</strong> Leveraged diverse interactions to gain new perspectives that enhanced the project's impact and direction.
+ <b>Broader Perspectives:</b> Leveraged diverse interactions to gain new perspectives that enhanced the project's impact and direction.
</li>
</ul>],
months: "September"