From 78aba5a385ea517bc421cf6c6a9360d2a61d3033 Mon Sep 17 00:00:00 2001
From: Isabell Guckes <isabell.guckes@uni-bielefeld.de>
Date: Wed, 6 Nov 2024 12:06:22 +0100
Subject: [PATCH] correction 2
---
src/contents/methods.tsx | 8 ++++----
1 file changed, 4 insertions(+), 4 deletions(-)
diff --git a/src/contents/methods.tsx b/src/contents/methods.tsx
index 316c8378..657dfb38 100644
--- a/src/contents/methods.tsx
+++ b/src/contents/methods.tsx
@@ -22,14 +22,14 @@ export function Methods() {
<p>Patch clamp recording involves the use of a glass micropipette which is manufactured from a glass capillary through the use of a Micropipette Puller. The micropipette is then filled with an electrolyte solution, which is subsequently brought into contact with the cell membrane. By applying gentle suction, a high-resistance seal called giga seal is formed between the pipette tip and the membrane patch. This enables the measurement of ionic currents with minimal noise interference [3]. <strong>Whole-Cell Configuration</strong> records currents from the entire cell by rupturing the membrane patch, accessing the intracellular environment, and is useful for analysing overall ion channel activity and cellular responses. <strong>Single-Channel Recording</strong> measures currents through individual ion channels without rupturing the membrane, enabling high-resolution study of channel conductance, gating, and selectivity [2].</p>
<figure>
<iframe title="Bielefeld-CeBiTec: Patch Clamp Measurement (2024)" width="560" height="315" src="https://video.igem.org/videos/embed/0d948e57-5997-430a-a2df-815b71a2fc67?autoplay=1" frameBorder="0" allowFullScreen={true} sandbox="allow-same-origin allow-scripts allow-popups allow-forms"></iframe>
- <figcaption> <b>Figure 1.</b> Microscopic recording of micropipette sealing of a HEK293 cell </figcaption>
+ <figcaption> <b>Figure 1. </b> Microscopic recording of micropipette sealing of a HEK293 cell. </figcaption>
</figure>
<p>The success of patch clamp experiments heavily depends on the composition of the solutions used. Typically, two main types of solutions are employed: The <strong>Pipette Solution</strong> in the micropipette mimics the intracellular environments, while the <strong>Bath Solution</strong> surrounds the cell and usually contains components that replicate the extracellular environment. Both solutions are meticulously designed to reflect the physiological conditions under which the cells operate, thereby ensuring that the measurements accurately reflect ion channel activity in a natural setting [2].</p>
<figure>
<img src="https://static.igem.wiki/teams/5247/photos/for-wiki-texts/meth-patch-clamp/bild-meth-patch-clamp.png" alt="Patch clamp setup"/>
- <figcaption><b>Figure 2.</b> Patch clamp setup</figcaption>
+ <figcaption><b>Figure 2. </b> Patch clamp setup.</figcaption>
</figure>
</Subesction>
<Subesction title="Application in CFTR gene prime editing validation" id="Patch Clamp3">
@@ -79,7 +79,7 @@ export function Methods() {
<img src="https://static.igem.wiki/teams/5247/integrated-human-practices/mttassay.webp" alt="PC1" style={{maxHeight: "200pt"}}/>
<figcaption>
<b>Figure 6. </b>
- MTT Assay: formation of purple formazan crystals by living cells.
+ MTT Assay: Formation of purple formazan crystals by living cells.
</figcaption>
</figure>
</div>
@@ -115,7 +115,7 @@ In addition to Cryo-EM, we employed scanning electron microscopy (SEM) to furthe
<img src="https://static.igem.wiki/teams/5247/delivery/plasmatem.webp" alt="PC1" style={{maxHeight: "200pt"}}/>
<figcaption>
<b>Figure 7. </b>
- Sample preparation for SEM: sputtering in Argon plasma.
+ Sample preparation for SEM: Sputtering in Argon plasma.
</figcaption>
</figure>
</div>
--
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