From 7392f55b776587d71267ef9b386e5f2261abaed4 Mon Sep 17 00:00:00 2001
From: Isabell Guckes <isabell.guckes@uni-bielefeld.de>
Date: Tue, 1 Oct 2024 17:44:00 +0200
Subject: [PATCH] bild tem
---
src/contents/methods.tsx | 22 ++++++++++++++++------
1 file changed, 16 insertions(+), 6 deletions(-)
diff --git a/src/contents/methods.tsx b/src/contents/methods.tsx
index 7b63f4ad..5d47b8f5 100644
--- a/src/contents/methods.tsx
+++ b/src/contents/methods.tsx
@@ -84,14 +84,24 @@ export function Methods() {
</Subesction>
<Subesction title="In-Depth Characterization of LNPs" id="In-Depth Characterization of LNPs">
- <H4 text="Dynamic Light Scattering (DLS) and Zeta Potential"></H4>
- <p>We used dynamic light scattering (DLS) to measure the size distribution and polydispersity index (PDI) of our LNPs. This technique allowed us to confirm that the LNPs had a consistent size distribution with minimal aggregation, which is essential for their stability. Additionally, we measured the zeta potential of the LNPs to assess their surface charge. A high zeta potential confirmed that the LNPs were stable in suspension, which is critical for their effectiveness in biological environments. </p>
- <H4 text="SEM and Cryo-EM for Structural Analysis"></H4>
+ <H4 text="Dynamic Light Scattering (DLS) and Zeta Potential"></H4>
+ <p>We used dynamic light scattering (DLS) to measure the size distribution and polydispersity index (PDI) of our LNPs. This technique allowed us to confirm that the LNPs had a consistent size distribution with minimal aggregation, which is essential for their stability. Additionally, we measured the zeta potential of the LNPs to assess their surface charge. A high zeta potential confirmed that the LNPs were stable in suspension, which is critical for their effectiveness in biological environments. </p>
+ <H4 text="SEM and Cryo-EM for Structural Analysis"></H4>
+ <div className='row align-items-center'>
+ <div className='col'>
<p>To further characterize the morphology and surface structure of the LNPs, we employed scanning electron microscopy (SEM). SEM provided high-resolution images that confirmed the spherical shape and uniformity of the LNPs. Additionally, cryo-electron microscopy (cryo-EM) allowed us to investigate the internal structure of the LNPs, revealing the presence of lipid layers and encapsulated materials, which are crucial for understanding their function in drug delivery. </p>
- {/* <H4 text="DNase Assay for Stability of Encapsulated Material "></H4>
- <p>Finally, we conducted a DNase assay to evaluate whether the LNPs could protect encapsulated nucleic acids, such as mRNA, from enzymatic degradation. This assay demonstrated that the LNPs successfully shielded the genetic material, ensuring its stability until it reaches target cells. </p> */}
+ </div>
+ <div className='col'>
+ <figure>
+ <img src="https://static.igem.wiki/teams/5247/delivery/plasmatem.webp" alt="PC1" style={{maxHeight: "200pt"}}/>
+ <figcaption>
+ <b>Figure 6. </b>
+ Sample preparation for SEM: sputtering in Argon plasma.
+ </figcaption>
+ </figure>
+ </div>
+ </div>
</Subesction>
-
<Subesction title="Conclusion" id="Conclusion">
<H4 text="Importance of Safety in LNP Development"></H4>
<p>Testing the safety of our LNPs was a critical step in their development. LNPs are increasingly being used in cutting-edge therapies, such as mRNA vaccines and targeted drug delivery systems. For these technologies to be viable, the nanoparticles must not harm the cells they are intended to interact with. The MTT and proliferation assays provided robust data, confirming the biocompatibility of our LNPs and reinforcing their potential for safe use in further research and clinical applications. </p>
--
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