From 71e2f8214a40d5bcb6c2fc9b630917210a1944a7 Mon Sep 17 00:00:00 2001
From: Isabell Guckes <isabell.guckes@uni-bielefeld.de>
Date: Wed, 2 Oct 2024 08:00:49 +0200
Subject: [PATCH] fix del
---
src/contents/engineering.tsx | 4 ++--
1 file changed, 2 insertions(+), 2 deletions(-)
diff --git a/src/contents/engineering.tsx b/src/contents/engineering.tsx
index d4a2898c..c310d865 100644
--- a/src/contents/engineering.tsx
+++ b/src/contents/engineering.tsx
@@ -1086,7 +1086,7 @@ export function Engineering() {
<p>The final LNP solution can be stored at 4 °C for a few days. It is recommended to use the formulated LNPs as soon as possible to maintain consistent results. Storage at RT is not recommended. Storage at freezing temperatures is also not recommended unless optimized cryoprotectants are used.</p>
</Collapsible>
- <p>The final innovation for our LNP to become <strong>AirBuddy</strong> came through consultation with Benjamin Winkeljann from <a onClick={() => goToPagesAndOpenTab('rnhale', '/human-practices')}> RNhale</a>, where the use of spray-drying techniques was discussed. Spray-drying the LNPs, instead of using traditional methods, helped improve stability and eco-friendliness of the product<TabScrollLink tab="tab-delivery" num="8" scrollId="desc-8"/>. The spray-dried SORT LNPs demonstrated lower cytotoxicity, and the technique proved effective in maintaining particle integrity. In conclusion, we created a stable LNP for efficient delivery of mRNA therapeutics to the lungs since the successful delivery of the prime editing complex via inhalation is key to our project. </p>
+ <p>The final innovation for our LNP to become <strong>AirBuddy</strong> came through consultation with Benjamin Winkeljann from <a onClick={() => goToPagesAndOpenTab('rnhale', '/human-practices')}> RNhale</a>, where the use of spray-drying techniques was discussed. Spray-drying the LNPs, instead of using traditional methods, helps improve stability and eco-friendliness of the product<TabScrollLink tab="tab-delivery" num="8" scrollId="desc-8"/>. Unfortunately, we were not in time for our samples to be delivered to RNhale for spray-drying before the wiki freeze, but we have already scheduled the transfer. Instead, the discussion with <a onClick={() => goToPagesAndOpenTab('moorlach', '/human-practices')}>Benjamin Moorlach</a>, chitosan expert working at FH Bielefeld, provided new ideas for improvement by <strong>complexing the mRNA with chitosan</strong> to improve the stability of the cargo during spray drying and nebulization. The positive effect of RNA chitosan complexing for opimized LNP delivery could be confimed in our lab. In conclusion, we created a stable LNP for efficient delivery of mRNA therapeutics to the lungs since the successful delivery of the prime editing complex via inhalation is key to our project. </p>
<figure>
<img src="https://static.igem.wiki/teams/5247/delivery/big-plan-inhalation-teil-del.webp" alt="Flow DEL"/>
<figcaption>
@@ -1100,7 +1100,7 @@ export function Engineering() {
<div className="box" >
<p id="del5">
<H3 text="Outlook" id="del5head" />
- Ultimately, through continuous cycles of experimentation, feedback, and optimization, a LNP formulation called AirBuddy was designed using SORT LNPs and a spray-drying process, achieving lung specificity and improved safety. We also want to state that for our LNP is further room for improvement. Intensive research led us to the realization that, among other modifications, <strong>antibody conjugation</strong> as a surface modification of our LNP for cell type-specific administration, more specifically club cells<TabScrollLink tab="tab-delivery" num="9" scrollId="desc-9"/> and ionocytes<TabScrollLink tab="tab-delivery" num="10" scrollId="desc-10"/> as most CFTR-expressing lung epithelial cells, would round off our most important aspect of precision. In addition, the discussion with <a onClick={() => goToPagesAndOpenTab('moorlach', '/human-practices')}>Benjamin Moorlach</a>, chitosan expert working at FH Bielefeld, provided new ideas for improvement by <strong>complexing the mRNA with chitosan</strong> to improve the stability of the cargo during spray drying and nebulization.
+ Ultimately, through continuous cycles of experimentation, feedback, and optimization, a LNP formulation called AirBuddy was designed using SORT LNPs and a spray-drying process, achieving lung specificity and improved safety. We also want to state that for our LNP is further room for improvement. Intensive research led us to the realization that, among other modifications, <strong>antibody conjugation</strong> as a surface modification of our LNP for cell type-specific administration, more specifically club cells<TabScrollLink tab="tab-delivery" num="9" scrollId="desc-9"/> and ionocytes<TabScrollLink tab="tab-delivery" num="10" scrollId="desc-10"/> as most CFTR-expressing lung epithelial cells, would round off our most important aspect of precision.
</p>
</div>
<Section title="References" id="references">
--
GitLab