From 46379541e6f7af70056f547eca0f2572f64542b7 Mon Sep 17 00:00:00 2001
From: Kathleen Susat <kathleen.susat@uni-bielefeld.de>
Date: Sun, 1 Dec 2024 16:14:54 +0100
Subject: [PATCH] HE PAS results
---
src/contents/results.tsx | 21 +++++++++++++++++----
1 file changed, 17 insertions(+), 4 deletions(-)
diff --git a/src/contents/results.tsx b/src/contents/results.tsx
index 1f99385..dab15ab 100644
--- a/src/contents/results.tsx
+++ b/src/contents/results.tsx
@@ -496,7 +496,20 @@ export function Results() {
<p>Characterisation of the cells in both culture systems was performed by a combination of microscopic examination and histological staining. In particular, haematoxylin-eosin (HE) and periodic acid-Schiff (PAS) staining was performed in the ALI culture to analyse cell differentiation and the formation of mucus-forming goblet cells. HE staining was used for general visualisation of cell morphology and cell stratification, while PAS staining was used specifically to identify the mucins in the goblet cells. These histological techniques allowed a detailed assessment of epithelial differentiation, in particular the maturation of ciliated and mucus-producing cells. </p>
<p>Once the cell cultures had been successfully established, a key objective of the project was pursued: To test gene our therapy approach in these cell models. The cells would be used to assess the efficacy and safety of new gene therapies before they could potentially be used in clinical applications. </p>
<H4 text="Conclusion"/>
- <p>The <strong>successful cultivation of primary human nasal epithelial cells (hNECs)</strong> was clearly demonstrated in both two-dimensional and three-dimensional cultures. In the ALI culture, a well-differentiated ciliated epithelium was observed microscopically, exhibiting the characteristic ciliary dynamics. Histological staining, in particular HE and PAS staining, provided confirmation of the differentiation of the cells. The multilayered epithelial structure and the presence of mucus-producing goblet cells were both clearly demonstrated. </p>
+ <div className="row align-items-center">
+ <div className="col">
+
+ <OneFigure
+ pic1="https://static.igem.wiki/teams/5247/photos/results/a.webp"
+ num={35}
+ description="Histochemical staining of air liquid interface culture. A) HE staining with goblet cell visible. B) HE staining with goblet cell visible. C) Overview of differnetiated epithelium after PAS staining"
+ alt1="HE and PAS staining"
+ />
+ </div>
+ <div className="col">
+ <p>The <strong>successful cultivation of primary human nasal epithelial cells (hNECs)</strong> was clearly demonstrated in both two-dimensional and three-dimensional cultures. In the ALI culture, a well-differentiated ciliated epithelium was observed microscopically, exhibiting the characteristic ciliary dynamics. Histological staining, in particular HE and PAS staining, provided confirmation of the differentiation of the cells. The multilayered epithelial structure and the presence of mucus-producing goblet cells were both clearly demonstrated (Figure 38 A, B). </p>
+ </div>
+ </div>
<p>The apical out-airway organoid culture also demonstrated successful differentiation of the cells, which formed an epithelial cell network. The cells exhibited a spherical morphology and were observed to float in the culture, thereby mimicking the natural physiological conditions of the respiratory epithelium. The various 2D and 3D models thus provide a promising foundation for further research and the application of gene therapy approaches. </p>
</Subesction >
@@ -507,7 +520,7 @@ export function Results() {
<div className='col'>
<OneFigure
pic1="https://static.igem.wiki/teams/5247/photos/results/patchclamp/pc.webp"
- num={35}
+ num={37}
bg="white"
description="Current density of HEK293, HEK293T CFTR WT and HEK293T CFTR F508del showing significant differences of both HEK293T cell lines compared to HEK293 but no significant differences between them. For statistics one-way ANOVA was performed."
alt1="Current density differences between HEK293 and HEK293T CFTR cell lines"
@@ -526,7 +539,7 @@ export function Results() {
<OneFigure
alt1=""
pic1="https://static.igem.wiki/teams/5247/photos/results/patchclamp/pc2.webp"
- num={36}
+ num={38}
bg="white"
description="
Repeated validation of current density measurements in HEK293T CFTR WT and HEK293T CFTR-F508del, showing consistent results with the initial experiment. Mean +/- SEM for n=5. For statistics one-way ANOYA was performed"
@@ -534,7 +547,7 @@ export function Results() {
<OneFigure
pic1=""
alt1="PC1"
- num={37}
+ num={39}
description=""
/>
</div>
--
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