diff --git a/src/App/LandingPage.css b/src/App/LandingPage.css
index 0f71a240c7d0fa7b04a8f73ed7045b3bc85ed25a..c893096a5ea44b1e89d32fb197de76d8132cfde6 100644
--- a/src/App/LandingPage.css
+++ b/src/App/LandingPage.css
@@ -54,4 +54,19 @@
background-size: 100% auto;
background-repeat: no-repeat;
padding: 0 !important;
+}
+
+.button-x{
+
+ align-items: center;
+ display: flex;
+ align-self: center;
+}
+
+.button-x button{
+ margin: auto;
+ padding: 10px;
+ border-radius: 10px;
+ background-color: var(--text-primary);
+ color: var(--ourbeige)
}
\ No newline at end of file
diff --git a/src/contents/Home.tsx b/src/contents/Home.tsx
index 64920a3303f635b36ccb01ae568eeb465b3c7345..0a86d2c3e275db32cfe6e174f3026919e6aa580f 100644
--- a/src/contents/Home.tsx
+++ b/src/contents/Home.tsx
@@ -7,7 +7,6 @@ import { useTabNavigation } from "../utils/TabNavigation";
import { H5 } from "../components/Headings";
import PreCyse from "../components/precyse";
import { useNavigation } from "../utils";
-import SVGComponentOne from "./Home Folder/svg-one";
export function Home() {
useTabNavigation();
@@ -61,6 +60,19 @@ export function Home() {
</div>
</div>
+ <img src="https://static.igem.wiki/teams/5247/landing-page/lp-2.svg"/>
+ <div className="row">
+ <div className="col button-x">
+ <button onClick={() => goToPageAndScroll("Approach2H", "/description")} > Lung-Specific Lipid Nanoparticle</button>
+ </div>
+ <div className="col button-x">
+ <button onClick={() => goToPageAndScroll("Approach1H", "/description")} > Next-Generation Prime Editing Technology
+ </button>
+ </div>
+ </div>
+
+
+
<div className="row mt-4">
<div className="col" id="erstecol">
<div className="col">
diff --git a/src/contents/description.tsx b/src/contents/description.tsx
index 6d54e20e721983a1c85f567cfe856d2168697a35..1202bb2413622005ebe5cf1b961a75b1cb97ca0e 100644
--- a/src/contents/description.tsx
+++ b/src/contents/description.tsx
@@ -288,8 +288,8 @@ export function Description() {
<p>All these challenges complicate the work with LNPs and present scientists with a great challenge, which makes working with LNPs even more important to find solutions.</p>
</Collapsible>
<br/>
- <div className='row align-items-center'>
- <p>To optimize AirBuddy for pulmonary delivery, we collaborated extensively with several experts, including <a onClick={() => goToPagesAndOpenTab('weber', '/human-practices')}>Prof. Weber, Dr. Große-Onnebrink</a> and <a onClick={() => goToPagesAndOpenTab('kolonkofirst', '/human-practices')}>Dr. Kolonko</a> as medical experts, <a onClick={() => goToPagesAndOpenTab('kristian', '/human-practices')}>Prof. Dr. Müller</a>, <a onClick={() => goToPagesAndOpenTab('radukic', '/human-practices')}>Dr. Radukic</a>, <a onClick={() => goToPagesAndOpenTab('moorlach', '/human-practices')}>Benjamin Moorlach</a> and the <a onClick={() => goToPagesAndOpenTab('biophysik', '/human-practices')}>Physical and Biophysical Chemistry working group</a> as academic experts form Bielefeld University and FH Bielefeld as well as <a onClick={() => goToPagesAndOpenTab('corden', '/human-practices')}>Corden Pharma</a> and <a onClick={() => goToPagesAndOpenTab('rnhale', '/human-practices')}>RNhale</a> as industrial experts. Throughout the <a onClick={() => goToPagesAndOpenTab('delivery head', '/engineering')}>development process</a>, we tested two commercially available kits: the <strong>Cayman Chemical LNP Exploration Kit (LNP-102)</strong> and the <strong>Corden Pharma LNP Starter Kit #2</strong>. While the Cayman kit demonstrated limited transfection efficiency, the Corden Pharma formulation significantly enhanced cellular uptake in lung tissues. Building on this, we integrated the <strong>SORT LNP</strong> method based on Wang's research <SupScrollLink label="1"/> , making our nanoparticles lung-specific. Additionally, we are employing the <strong>spray-drying technique</strong> in cooperation with RNhale <SupScrollLink label="2"/> to improve the stability of our LNP and the <strong>complexation of mRNA with chitosan</strong>, ensuring that the LNP and it's cargo withstands the inhalation process without degradation. This stability is crucial for the efficient delivery of mRNA into lung epithelial cells, where PrimeGuide can effectively perform genome editing.</p>
+ <div id="airbuddy-hook" className='row align-items-center'>
+ <p>To optimize AirBuddy for pulmonary delivery, we collaborated extensively with several experts, including <a onClick={() => goToPagesAndOpenTab('weber', '/human-practices')}>Prof. Weber, Dr. Große-Onnebrink</a> and <a onClick={() => goToPagesAndOpenTab('kolonkofirst', '/human-practices')}>Dr. Kolonko</a> as medical experts, <a onClick={() => goToPagesAndOpenTab('kristian', '/human-practices')}>Prof. Dr. Müller</a>, <a onClick={() => goToPagesAndOpenTab('radukic', '/human-practices')}>Dr. Radukic</a>, <a onClick={() => goToPagesAndOpenTab('moorlach', '/human-practices')}>Benjamin Moorlach</a> and the <a onClick={() => goToPagesAndOpenTab('biophysik', '/human-practices')}>Physical and Biophysical Chemistry working group</a> as academic experts form Bielefeld University and FH Bielefeld as well as <a onClick={() => goToPagesAndOpenTab('corden', '/human-practices')}>Corden Pharma</a> and <a onClick={() => goToPagesAndOpenTab('rnhale', '/human-practices')}>RNhale</a> as industrial experts. Throughout the <a onClick={() => goToPagesAndOpenTab('delivery head', '/engineering')}>development process</a>, we tested two commercially available kits: the <strong>Cayman Chemical LNP Exploration Kit (LNP-102)</strong> and the <strong>Corden Pharma LNP Starter Kit #2</strong>. While the Cayman kit demonstrated limited transfection efficiency, the Corden Pharma formulation significantly enhanced cellular uptake in lung tissues. Building on this, we integrated the <strong>SORT LNP</strong> method based on Wang's research <SupScrollLink label="1"/> , making our nanoparticles lung-specific. Additionally, we employed the <strong>spray-drying technique</strong> in cooperation with RNhale <SupScrollLink label="2"/> to improve the stability of our LNP, ensuring that it withstands the inhalation process without degradation. This stability is crucial for the efficient delivery of mRNA into lung epithelial cells, where PrimeGuide can effectively perform genome editing.</p>
<img src="https://static.igem.wiki/teams/5247/delivery/big-plan-inhalation-teil-del.webp"/>
</div>
<p>To evaluate the <strong>delivery efficiency</strong>, we transfected HEK292 and CFBE41o- cells using fluorescent cargo and quantified the results through FACS analysis. We also ensured that AirBuddy meets the necessary standards for safety and efficacy since we conducted extensive <a onClick={() => goToPageAndScroll ('In-Depth Characterization of LNPsH', '/materials-methods')}> characterization of the LNPs </a>using techniques such as Zeta potential analysis, Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM), and Cryogenic Electron Microscopy (cryo-EM). These methods confirmed the uniformity, stability, and optimal size distribution of the nanoparticles. Furthermore, <strong>cytotoxicity assessments</strong> including MTT and proliferation assays demonstrated that our LNPs are biocompatible and do not impede cell growth or function by the incorporation of <a onClick={() => goToPagesAndOpenTab('it4', '/engineering')}>PEG</a> and other ambivalent components. These findings reinforce AirBuddy's potential as a safe and effective tool for pulmonary delivery, with broad implications for gene therapies targeting lung diseases.</p>
diff --git a/src/contents/engineering.tsx b/src/contents/engineering.tsx
index 3a153f2a32205e94c340c4c8f1637bc4776d67dd..a18b18c54c7fb88dc660148b2c62064ce549bbb6 100644
--- a/src/contents/engineering.tsx
+++ b/src/contents/engineering.tsx
@@ -272,15 +272,15 @@ export function Engineering() {
</p>
<H4 text="Build" id="text"/>
<p>
- The protocol entailed the utilization of varying concentrations of Lipofectamine 3000, specifically 1 µl and 1.5 µl, with a DNA quantity of 1 µg or 0.5 µg.
- </p>
+ The protocol entailed the utilization of varying concentrations of Lipofectamine 3000, specifically 1 µl and 1.5 µl, with a DNA quantity of 1 µg or 0.5 µg. In this phase, we developed the transfection method with calcium chloride (CaCl2) as an alternative to conventional lipofectamine transfection. The aim was to test whether this more cost-effective method offers comparable transfection efficiency. Three different DNA concentrations were used to investigate the effect on transfection efficiency.
+ </p>
<H4 text="Test" id="text"/>
<p>
- To enhance transfection efficiency, optimization tests were conducted, in which the quantities of Lipofectamine and DNA were varied. The objective of this iteration was to find the optimal ratio of Lipofectamine 3000 to DNA. To this end, 1 µl and 1.5 µl of Lipofectamine 3000 at a DNA concentration of either 1 µg or 0.5 µg were compared with each other.
+ To enhance transfection efficiency, optimization tests were conducted, in which the quantities of Lipofectamine and DNA were varied. The objective of this iteration was to find the optimal ratio of Lipofectamine 3000 to DNA. To this end, 1 µl and 1.5 µl of Lipofectamine 3000 at a DNA concentration of either 1 µg or 0.5 µg were compared with each other. In the next step, the tests were carried out with the different DNA concentrations using the CaCl2 transfection method. The transfection efficiencies were compared with those from the Lipofectamine transfection to determine whether the new method represents an improvement.
</p>
<H4 text="Learn" id="text"/>
<p>
- The experiment demonstrated that a quantity of 1 µl Lipofectamine 3000 was sufficient for successful transfection, and that increasing the quantity does not result in a notable difference. Additionally, the findings indicated that an amount of 1 µg DNA exhibited a higher efficiency than an amount of 0.5 µg DNA. It can be reasoned that additional factors may have contributed to the previously observed decline in transfection efficiency. One potential explanation is that the cells may have been in an excessively high passage level.
+ The experiment demonstrated that a quantity of 1 µl Lipofectamine 3000 was sufficient for successful transfection, and that increasing the quantity does not result in a notable difference. Additionally, the findings indicated that an amount of 1 µg DNA exhibited a higher efficiency than an amount of 0.5 µg DNA. It can be reasoned that additional factors may have contributed to the previously observed decline in transfection efficiency. One potential explanation is that the cells may have been in an excessively high passage level. It became clear from the tests that CaCl2 transfection did not deliver better results than Lipofectamine transfection. On the contrary, the efficiency was significantly lower, although the method is less expensive. This led to the realisation that the CaCl2 technique in this form was not a suitable alternative for our specific requirements.
</p>
<p>
It can be reasonably deduced that the aforementioned factors may have contributed to the observed decline in transfection efficiency.