From 3dd672db54fc2f8a674b81425a01306e4db1a93a Mon Sep 17 00:00:00 2001
From: liliana <liliana.sanfilippo@uni-bielefeld.de>
Date: Thu, 22 Aug 2024 21:14:22 +0200
Subject: [PATCH] patchclamp
---
src/App/App.css | 4 ++-
src/contents/human-practices.tsx | 42 +++++++++++++++++++++-----------
2 files changed, 31 insertions(+), 15 deletions(-)
diff --git a/src/App/App.css b/src/App/App.css
index 14564710..333731dd 100644
--- a/src/App/App.css
+++ b/src/App/App.css
@@ -67,7 +67,9 @@
.col-1{
width: 8.33333333% !important;
}
-
+.col2punkt5{
+ width: 20% !important;
+}
.col-max-10{
flex: 0 0 auto;
max-width: 83.33333333% !important;
diff --git a/src/contents/human-practices.tsx b/src/contents/human-practices.tsx
index 6289f72e..c5f46e2c 100644
--- a/src/contents/human-practices.tsx
+++ b/src/contents/human-practices.tsx
@@ -210,6 +210,8 @@ let timelinepersontabs =[
<img className="middle sechpro" src="https://static.igem.wiki/teams/5247/placeholders/placehilderperson.jpeg"/>
</div>
</div>
+ <img className="img-right img-half" src="https://static.igem.wiki/teams/5247/photos/for-wiki-texts/hp-patch-clamp/20240625-184032.jpg"/>
+ <div>
<h4>Aim of contact</h4>
<p>As part of our project, we aimed to demonstrate the functionality of the CFTR ion channel, after restoring
it through our optimized Prime Editing, by using Patch-Clamp measurements. To ensure the optimal use of the
@@ -220,9 +222,15 @@ let timelinepersontabs =[
Neurophysiology working group at Bielefeld University, seemed to be an ideal interviewee. His
knowledge and experience promised valuable insights and advice for conducting and optimizing our
experiments. </p>
- <img src="https://static.vecteezy.com/system/resources/previews/016/916/479/original/placeholder-icon-design-free-vector.jpg" style={{width: "30%"}}/>
- <h4>Insights</h4>
- <p>Prof. Dr. Wischmeyer taught us about the workflow of the Patch-Clamp technique. He highlighted the need
+ </div>
+ <br/>
+ <div className="row">
+ <div className="col2punkt5">
+ <img src="https://static.igem.wiki/teams/5247/photos/for-wiki-texts/hp-patch-clamp/bild-patch-clamp-isi-oliver.jpeg" />
+ </div>
+ <div className="col">
+ <h4>Insights</h4>
+ <p>Prof. Dr. Wischmeyer taught us about the workflow of the Patch-Clamp technique. He highlighted the need
for specialized electrodes and glass pipettes that must form a smooth surface devoid of the extracellular
matrix (ECM). Additionally, he pointed out that measuring CFTR conductivity with the Patch-Clamp technique
poses a technical challenge due to the low currents involved<a href="desc-2"><sup>2</sup></a>. He recommended using expression vectors
@@ -235,25 +243,31 @@ let timelinepersontabs =[
current electrophysiological measurement method alongside the Patch-Clamp technique. This method allows
the measurement of the membrane potential above and below a monolayer of confluent cells<a href="desc-3"><sup>3</sup></a>. Consequently,
it enables precise measurement of conductivity dependent on CFTR expression. </p>
- <h4>Implementation</h4>
- <p>We decided to use HEK293T cells lines from Mattijs Bulcaen from KU Leuven [Link] which do overexpress the
+ </div>
+ </div>
+
+
+ <h4>Implementation</h4>
+ <div className="row align-items-center">
+ <div className="col">
+ <p>We decided to use HEK293T cells lines from Mattijs Bulcaen from KU Leuven [Link] which do overexpress the
correct CFTR and those which express CFTR with F508del for the Patch-Clamp measurements. To conduct the
Patch-Clamp experiments, we contacted the Cellular Neurophysiology group to perform the necessary
measurements. It was a pleasure to work together with Dr. Oliver Dräger, who is working as a post-doc for
the Cellular Neurophysiology working group at Bielefeld University. He taught us about the Patch-Clamp
method and spent his valuable time supporting our project by guiding our Patch-Clamp measurements. </p>
- <div className="row align-items-center">
- <div className="col">
- <img src="https://static.vecteezy.com/system/resources/previews/016/916/479/original/placeholder-icon-design-free-vector.jpg" style={{width: "30%"}}/>
- </div>
- <div className="col">
- <img src="https://static.vecteezy.com/system/resources/previews/016/916/479/original/placeholder-icon-design-free-vector.jpg" style={{width: "30%"}}/>
- </div>
- </div>
- <p>In summary, through the interview with Prof. Dr. Wischmeyer and the collaboration with his employee
+ <p>In summary, through the interview with Prof. Dr. Wischmeyer and the collaboration with his employee
Oliver Dräger, we gained valuable insights and optimized our approach to effectively investigate and
measure the functionality of the CFTR ion channel, thereby determining the efficiency of our Prime
Editing strategy. </p>
+ </div>
+ <div className="col-5">
+ <img src="https://static.igem.wiki/teams/5247/photos/for-wiki-texts/hp-patch-clamp/bild-interssierte-wissenschaftler-oho.jpeg" style={{maxHeight: "300px"}}/>
+ </div>
+ </div>
+
+
+
<h4>References</h4>
{/*<!-- Citation num 1--> */}
<li typeof="schema:ScolarlyArticle" role="doc-biblioentry" property="schema:citation" id="desc-1">
--
GitLab