From 350c2bec8d6a564efeeb7da7f88f567d5fe78204 Mon Sep 17 00:00:00 2001
From: Liliana Sanfilippo <lsanfilippo@techfak.uni-bielefeld.de>
Date: Thu, 21 Nov 2024 17:07:29 +0100
Subject: [PATCH] PE6c statt P6C
---
src/contents/description.tsx | 2 +-
1 file changed, 1 insertion(+), 1 deletion(-)
diff --git a/src/contents/description.tsx b/src/contents/description.tsx
index d22e4bc1..5d8eb830 100644
--- a/src/contents/description.tsx
+++ b/src/contents/description.tsx
@@ -254,7 +254,7 @@ export function Description() {
<p><b>Improving Editing Efficiency: PBS and RTT length adjustments with Silent Edits</b></p>
<p>To maximize editing efficiency, various combinations of primer binding site (PBS) and reverse transcriptase template (RTT) lengths were evaluated. The RTT, which provides the template for the desired genetic change, was carefully optimized, including the introduction of silent edits—changes in the RTT that do not alter the protein sequence but can improve the editing process. Both shortened and extended versions of the PBS and RTT were tested in combinations with each other, with and without these silent edits, to identify the optimal configuration that would result in the highest editing efficiency. This step-by-step screening process allowed for the selection of the most efficient pegRNA for targeting the CFTR gene, ensuring that the system could achieve high levels of successful edits with minimal unintended consequences.</p>
</Collapsible>
- <p>For the reverse transcriptase, we selected the P6C variant, which has shown to provide the best editing efficiency and a more compact structure compared to alternatives. Its advanced development stage and ability to offer high precision and editing performance made it the ideal choice for Prime Guide. </p>
+ <p>For the reverse transcriptase, we selected the PE6c variant, which has shown to provide the best editing efficiency and a more compact structure compared to alternatives. Its advanced development stage and ability to offer high precision and editing performance made it the ideal choice for Prime Guide. </p>
<p>Together, these components form a highly optimized Prime Editing system that balances size, stability, and efficiency. Our aim with Prime Guide is to create a robust and precise solution for correcting the F508del mutation in Cystic Fibrosis, building on the guidance from our expert collaborators and extensive testing of each individual component.</p>
<H4 text="Our PreCyse cassette" id="PreCyse-cassette"/>
<p>We have developed our PrimeGuide, an optimized version of the Prime Editing system, designed to enhance editing efficiency, precision, and versatility. As part of our continued efforts to improve and streamline the Prime Editing workflow, we introduce to you the PreCyse-Cassette—a universal plasmid backbone specifically tailored for any Prime Editing system.</p>
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