From 227f97ee50c753540778b51a4bf85ea5d3100df3 Mon Sep 17 00:00:00 2001
From: Lisa Wiesner <lisa.wiesner@uni-bielefeld.de>
Date: Tue, 3 Dec 2024 13:50:16 +0000
Subject: [PATCH] Update file results.tsx
---
src/contents/results.tsx | 8 ++++----
1 file changed, 4 insertions(+), 4 deletions(-)
diff --git a/src/contents/results.tsx b/src/contents/results.tsx
index 9b620c5..80e2f03 100644
--- a/src/contents/results.tsx
+++ b/src/contents/results.tsx
@@ -566,10 +566,7 @@ export function Results() {
<Subesction title="Combining Primeguide and AirBuddy" id="Experimental Design6">
<H5 text="Transfection"/>
<div className='col'>
- <p>Fluorescence microscopy was performed using the Leica DMI6000 B microscope at 20x magnification to analyze CFBE4o- cells. These cells were initially pre-transfected with pPEAR_CFTR to establish a baseline for Prime Editing activity. Subsequently, transfection ofPE6cpegRNA4, delivered via the AirBuddy system, was carried out. LNPs without cargo acted as the negative control. Imaging was conducted at 24h, 48h, and 72h post-transfection to assess fluorescence intensity and evaluate editing efficiency over time.
- The results sho a sucsessful transfection of PE6c and pegRNA4 encapsulated in our LNPs was achieved following prior transfection with pPEAR_CFTR. The experimental setup demonstrated a significant increase in fluorescence intensity, indicative of enhanced Prime Editing efficiency under these conditions.
-Moreover, the PreCyse results confirm the effectiveness of our strategy, showcasing the synergy between our proprietary PrimeGuide system and the AirBuddy platform. This combination has proven to be pivotal in optimizing the editing outcomes and advancing the precision of the Prime Editing process.
-These findings underscore the robustness of our delivery approach and the innovative integration of the PrimeGuide and AirBuddy systems in driving Prime Editing to new levels of efficiency and accuracy.</p>
+ <p>Fluorescence microscopy was performed using the Leica DMI6000 B microscope at 20x magnification to analyze CFBE4o- cells. These cells were initially pre-transfected with pPEAR_CFTR to establish a baseline for Prime Editing activity. Subsequently, transfection ofPE6cpegRNA4, delivered via the AirBuddy system, was carried out. LNPs without cargo acted as the negative control. Imaging was conducted at 24h, 48h, and 72h post-transfection to assess fluorescence intensity and evaluate editing efficiency over time.</p>
</div>
<div className='col'>
<OneFigure
@@ -579,6 +576,9 @@ These findings underscore the robustness of our delivery approach and the innova
bg="white"
description="Microscopy of CFBE4o- 48h aftr transfection. Cells were previoulsy transfectet with pPEAR_CFTR and later tresnfected with PE6c+pegRNA4 via AirBuddy. BF = Brightfield "
/>
+ <p>The results show a sucsessful transfection of PE6c and pegRNA4 encapsulated in our LNPs was achieved following prior transfection with pPEAR_CFTR. The experimental setup demonstrated a significant increase in fluorescence intensity, indicative of enhanced Prime Editing efficiency under these conditions.
+Moreover, the PreCyse results confirm the effectiveness of our strategy, showcasing the synergy between our proprietary PrimeGuide system and the AirBuddy platform. This combination has proven to be pivotal in optimizing the editing outcomes and advancing the precision of the Prime Editing process.
+These findings underscore the robustness of our delivery approach and the innovative integration of the PrimeGuide and AirBuddy systems in driving Prime Editing to new levels of efficiency and accuracy.</p>
</div>
--
GitLab