diff --git a/code/bibtex.bib b/code/bibtex.bib
index 29da6e14203c160ad43ca7f369d1cdfca05b4a47..d958a04b4dee6504cd63e31f57283c5586a05608 100644
--- a/code/bibtex.bib
+++ b/code/bibtex.bib
@@ -1,77 +1,63 @@
-@article{article1, 
-author={Rodrigues, Roberta; Gabetta, Carmen S.; Pedro, Karla P.; Valdetaro, Fabio; Fernandes, Maria I. M.; Magalhães, PatrÃcia K. R.; Januário, José N., Maciel, Léa M. Z.}, 
+@article{article1,
-year={2008}, 
+author={Roth, F. C., Numberger, M., and Draguhn, A.},
-month={ }, 
+year={2023},
-pages={475-484}, 
+month={},
-title={Cystic fibrosis and neonatal screening}, 
+pages={1-14},
-volume={24}, 
+title={Die Entwicklung der Patch-Clamp-Technik},
-journal={Cadernos de Saúde Pública}, 
+volume={},
-doi={10.1590/S0102-311X2008001600002} 
+journal={Springer eBooks},
-} 
+doi={10.1007/978-3-662-66053-9_1}
-@article{article2, 
+}
-author={Farrell, Philip M.; Rock, Michael J.; Baker, Mei W.}, 
-year={2020}, 
+@article{article2,
-month={04}, 
+author={Mete, V.},
-pages={401}, 
+year={2023},
-title={The Impact of the CFTR Gene Discovery on Cystic Fibrosis Diagnosis, Counseling, and Preventive Therapy}, 
+month={ },
-volume={11(4)}, 
+pages={ },
-journal={Genes}, 
+title={Entwicklung und Validierung neuer nicht-invasiver Diagnosesysteme für Mucociliary Clearance Disorders (MCCD)},
-doi={10.3390/genes11040401} 
+volume={ },
-} 
-@article{article3, 
+journal={Dissertation, Westfälische Wilhelms-Universität Münster},
-author={Lukacs, Gergely L.; Verkman, A. S.}, 
+doi={10.17879/98958441905}
-year={2012}, 
+}
-month={2}, 
-pages={81-91}, 
-title={ CFTR: folding, misfolding and correcting the ΔF508 conformational defect}, 
+@article{article3,
-volume={18(2)}, 
+author={Giaever, I. and Keese, C. },
-journal={Trends in molecular medicine}, 
+year={1993},
-doi={10.1016/j.molmed.2011.10.003} 
+month={ },
-} 
-@article{article4, 
+pages={591-592},
-author={Amico, Giulia; Brandas, Chiara; Moran, Oscar; Baroni, Debora}, 
+title={A morphological biosensor for mammalian cells},
-year={2019}, 
+volume={366},
-month={1}, 
+journal={Nature},
-pages={5463}, 
+doi={10.1038/366591a0}
-title={Unravelling the Regions of Mutant F508del-CFTR More Susceptible to the Action of Four Cystic Fibrosis Correctors}, 
-
-volume={20(21)}, 
-
-journal={International Journal of Molecular Sciences}, 
-
-doi={10.3390/ijms20215463} 
-
-} 
\ No newline at end of file
+}
\ No newline at end of file
diff --git a/code/cit.py b/code/cit.py
index 3496dcd78720e5e03a2d979f4507143666f0cfdc..f10449ec6330c5c8b3cccbf25ded5d211c2f36b9 100644
--- a/code/cit.py
+++ b/code/cit.py
@@ -69,8 +69,8 @@ def main():
def articleHTML(dictio, x, out):
print("Writing html code for entry "+ str(x+1) + "...")
- out.write("{/*<!-- Citation num " + str(x+14) + "--> */}" + "\n")
- out.write("<li typeof=\"schema:ScolarlyArticle\" role=\"doc-biblioentry\" property=\"schema:citation\" id=\"desc-" + str(x+14) + "\">"+ "\n")
+ out.write("{/*<!-- Citation num " + str(x+1) + "--> */}" + "\n")
+ out.write("<li typeof=\"schema:ScolarlyArticle\" role=\"doc-biblioentry\" property=\"schema:citation\" id=\"desc-" + str(x+1) + "\">"+ "\n")
out.write("\t" + "<span property=\"schema:author\" typeof=\"schema:Person\">"+ "\n")
print("Just a sec, seperating authors...")
diff --git a/code/output.txt b/code/output.txt
index 75f9ceeae99847655e245348e2aa33a4b973cb99..aed766d1601c011c07e6b7db5dd59df875754c4d 100644
--- a/code/output.txt
+++ b/code/output.txt
@@ -1,52 +1,41 @@
-{/*<!-- Citation num 14--> */}
-<li typeof="schema:ScolarlyArticle" role="doc-biblioentry" property="schema:citation" id="desc-14">
+{/*<!-- Citation num 1--> */}
+<li typeof="schema:ScolarlyArticle" role="doc-biblioentry" property="schema:citation" id="desc-1">
<span property="schema:author" typeof="schema:Person">
- <span property="schema:Name"> Rodrigues, R.</span>
+ <span property="schema:Name"> Roth, F.</span>;
+ <span property="schema:Name"> Draguhn, A.</span>
</span>
- <span property="schema:name"> Cystic fibrosis and neonatal screening. </span>
- <i property="schema:publisher" typeof="schema:Organization"> Cadernos de Saúde Pública</i>
- <b property="issueNumber" typeof="PublicationIssue"> 24</b>,
- <span property="schema:pageBegin"> 475</span>-<span property="schema:pageEnd">484</span>
- (<time property="schema:datePublished" datatype="xsd:gYear" dateTime=" 2008">2008</time>).
- <a className="doi" href="https://doi.org/10.1590/S0102-311X2008001600002"> doi: 10.1590/S0102-311X2008001600002</a>
+ <span property="schema:name"> Die Entwicklung der Patch-Clamp-Technik. </span>
+ <i property="schema:publisher" typeof="schema:Organization"> Springer eBooks</i>
+ <b property="issueNumber" typeof="PublicationIssue"> </b>,
+ <span property="schema:pageBegin"> 1</span>-<span property="schema:pageEnd">14</span>
+ (<time property="schema:datePublished" datatype="xsd:gYear" dateTime=" 2023">2023</time>).
+ <a className="doi" href="https://doi.org/10.1007/978-3-662-66053-9_1"> doi: 10.1007/978-3-662-66053-9_1</a>
</li>
-{/*<!-- Citation num 15--> */}
-<li typeof="schema:ScolarlyArticle" role="doc-biblioentry" property="schema:citation" id="desc-15">
+{/*<!-- Citation num 2--> */}
+<li typeof="schema:ScolarlyArticle" role="doc-biblioentry" property="schema:citation" id="desc-2">
<span property="schema:author" typeof="schema:Person">
- <span property="schema:Name"> Farrell, P.</span>
+ <span property="schema:Name"> Mete, V.</span>
</span>
- <span property="schema:name"> The Impact of the CFTR Gene Discovery on Cystic Fibrosis Diagnosis, Counseling, and Preventive Therapy. </span>
- <i property="schema:publisher" typeof="schema:Organization"> Genes</i>
- <b property="issueNumber" typeof="PublicationIssue"> 11(4)</b>,
- <span property="schema:pageBegin">401</span>
- (<time property="schema:datePublished" datatype="xsd:gYear" dateTime=" 2020">2020</time>).
- <a className="doi" href="https://doi.org/10.3390/genes11040401"> doi: 10.3390/genes11040401</a>
+ <span property="schema:name"> Entwicklung und Validierung neuer nicht-invasiver Diagnosesysteme für Mucociliary Clearance Disorders (MCCD). </span>
+ <i property="schema:publisher" typeof="schema:Organization"> Dissertation, Westfälische Wilhelms-Universität Münster</i>
+ <b property="issueNumber" typeof="PublicationIssue"> </b>,
+ <span property="schema:pageBegin"> </span>
+ (<time property="schema:datePublished" datatype="xsd:gYear" dateTime=" 2023">2023</time>).
+ <a className="doi" href="https://doi.org/10.17879/98958441905"> doi: 10.17879/98958441905</a>
</li>
-{/*<!-- Citation num 16--> */}
-<li typeof="schema:ScolarlyArticle" role="doc-biblioentry" property="schema:citation" id="desc-16">
+{/*<!-- Citation num 3--> */}
+<li typeof="schema:ScolarlyArticle" role="doc-biblioentry" property="schema:citation" id="desc-3">
<span property="schema:author" typeof="schema:Person">
- <span property="schema:Name"> Lukacs, G.</span>
+ <span property="schema:Name"> Giaever, I.</span>;
+ <span property="schema:Name"> Keese, C.</span>
</span>
- <span property="schema:name"> CFTR: folding, misfolding and correcting the ΔF508 conformational defect. </span>
- <i property="schema:publisher" typeof="schema:Organization"> Trends in molecular medicine</i>
- <b property="issueNumber" typeof="PublicationIssue"> 18(2)</b>,
- <span property="schema:pageBegin"> 81</span>-<span property="schema:pageEnd">91</span>
- (<time property="schema:datePublished" datatype="xsd:gYear" dateTime=" 2012">2012</time>).
- <a className="doi" href="https://doi.org/10.1016/j.molmed.2011.10.003"> doi: 10.1016/j.molmed.2011.10.003</a>
-</li>
-
-{/*<!-- Citation num 17--> */}
-<li typeof="schema:ScolarlyArticle" role="doc-biblioentry" property="schema:citation" id="desc-17">
- <span property="schema:author" typeof="schema:Person">
- <span property="schema:Name"> Amico, G.</span>
- </span>
- <span property="schema:name"> Unravelling the Regions of Mutant F508del-CFTR More Susceptible to the Action of Four Cystic Fibrosis Correctors. </span>
- <i property="schema:publisher" typeof="schema:Organization"> International Journal of Molecular Sciences</i>
- <b property="issueNumber" typeof="PublicationIssue"> 20(21)</b>,
- <span property="schema:pageBegin">5463</span>
- (<time property="schema:datePublished" datatype="xsd:gYear" dateTime=" 2019">2019</time>).
- <a className="doi" href="https://doi.org/10.3390/ijms20215463"> doi: 10.3390/ijms20215463</a>
+ <span property="schema:name"> A morphological biosensor for mammalian cells. </span>
+ <i property="schema:publisher" typeof="schema:Organization"> Nature</i>
+ <b property="issueNumber" typeof="PublicationIssue"> 366</b>,
+ <span property="schema:pageBegin"> 591</span>-<span property="schema:pageEnd">592</span>
+ (<time property="schema:datePublished" datatype="xsd:gYear" dateTime=" 1993">1993</time>).
+ <a className="doi" href="https://doi.org/10.1038/366591a0"> doi: 10.1038/366591a0</a>
</li>
diff --git a/src/components/HorizontalTimeline.tsx b/src/components/HorizontalTimeline.tsx
index 37dc72c2493f04a481da78c5145fa657db01aab4..0cf09a757cadbee61814b8941ed0b092304aa1dc 100644
--- a/src/components/HorizontalTimeline.tsx
+++ b/src/components/HorizontalTimeline.tsx
@@ -363,7 +363,7 @@ function TimeItem({tag, title, pic, author, tabid}: ItemProps){
text: "Text",
},
{
- title: "" ,
+ title: "Research Group Cellular Neurophysiology" ,
pic: "https://static.igem.wiki/teams/5247/placeholders/placehilderperson.jpeg",
tag: "Academia",
author: "Prof. Dr. Erhard Wischmeyer",
@@ -450,6 +450,14 @@ function TimeItem({tag, title, pic, author, tabid}: ItemProps){
tabid: "InvBulcaen2",
text: "Text",
},
+ {
+ title: "Research Group Cellular Neurophysiology" ,
+ pic: "https://static.igem.wiki/teams/5247/placeholders/placehilderperson.jpeg",
+ tag: "Academia",
+ author: "Dr. Oliver Dräger",
+ tabid: "InvWischmeyer",
+ text: "Text",
+ },
diff --git a/src/contents/human-practices.tsx b/src/contents/human-practices.tsx
index 02b6e008c8024a45b809b4556ee6eeb65819d75a..6289f72ec3fddfba76e19760f70ea7a81e252b9c 100644
--- a/src/contents/human-practices.tsx
+++ b/src/contents/human-practices.tsx
@@ -98,7 +98,8 @@ let timelinepersontabs =[
Patient
</div>
</div>
- <div className="col">Original language: German</div>
+ <div className="col-3">Original language: German</div>
+ <div className="col"><button>Go to interview</button></div>
</div>
<div className="row">
<div className="col">
@@ -131,7 +132,8 @@ let timelinepersontabs =[
Patient
</div>
</div>
- <div className="col">Original language: German</div>
+ <div className="col-3">Original language: German</div>
+ <div className="col"><button>Go to interview</button></div>
</div>
<div className="row">
<div className="col">
@@ -163,7 +165,8 @@ let timelinepersontabs =[
Ethics Committee
</div>
</div>
- <div className="col">Original language: German</div>
+ <div className="col-3">Original language: German</div>
+ <div className="col"><button>Go to interview</button></div>
</div>
<div className="row">
<div className="col">
@@ -187,15 +190,17 @@ let timelinepersontabs =[
{
node: <>
<br/>
- <h3>Prof. Dr. Erhard Wischmeyer</h3>
+ <h3>Prof. Dr. Erhard Wischmeyer und Dr. Oliver Dräger </h3>
+ <h4>Research Group Cellular Neurophysiology, Bielefeld University</h4>
<hr/>
<div className="row">
<div className="col-2">
<div className="t-tag Academia">
- Beruf
+ Research Group
</div>
</div>
- <div className="col">Original language: German</div>
+ <div className="col-3">Original language: German</div>
+ <div className="col"><button>Got to Interview with Prof. Wischmeyer</button></div>
</div>
<div className="row">
<div className="col">
@@ -206,13 +211,92 @@ let timelinepersontabs =[
</div>
</div>
<h4>Aim of contact</h4>
- <p></p>
+ <p>As part of our project, we aimed to demonstrate the functionality of the CFTR ion channel, after restoring
+ it through our optimized Prime Editing, by using Patch-Clamp measurements. To ensure the optimal use of the
+ Patch-Clamp and to gain an insight into electrophysiology, we asked experts from the medical faculty at
+ Bielefeld University to critically examine our measurement planning. Prof. Dr. Erhard Wischmeyer, an
+ experienced scientist in this field who has worked at the Max Planck Institute for Biophysical Chemistry
+ in Göttingen, the development site of the Patch-Clamp technique<a href="desc-1"><sup>1</sup></a>, and currently leads the Cellular
+ Neurophysiology working group at Bielefeld University, seemed to be an ideal interviewee. His
+ knowledge and experience promised valuable insights and advice for conducting and optimizing our
+ experiments. </p>
+ <img src="https://static.vecteezy.com/system/resources/previews/016/916/479/original/placeholder-icon-design-free-vector.jpg" style={{width: "30%"}}/>
<h4>Insights</h4>
- <p></p>
- <h4>Clarification</h4>
- <p></p>
+ <p>Prof. Dr. Wischmeyer taught us about the workflow of the Patch-Clamp technique. He highlighted the need
+ for specialized electrodes and glass pipettes that must form a smooth surface devoid of the extracellular
+ matrix (ECM). Additionally, he pointed out that measuring CFTR conductivity with the Patch-Clamp technique
+ poses a technical challenge due to the low currents involved<a href="desc-2"><sup>2</sup></a>. He recommended using expression vectors
+ for overexpressing the CFTR gene in HEK cells instead of epithelial cells from a nasal swab to achieve
+ better results. Since Patch-Clamp measurements require a very sensitive testing environment, even
+ challenging for the most experienced scientists, Prof. Dr. Wischmeyer invited us to conduct the
+ measurements together with members of his group.
+ </p>
+ <p>In addition to the Patch-Clamp technique, Prof. Dr. Wischmeyer informed us about E-cis measurements as a
+ current electrophysiological measurement method alongside the Patch-Clamp technique. This method allows
+ the measurement of the membrane potential above and below a monolayer of confluent cells<a href="desc-3"><sup>3</sup></a>. Consequently,
+ it enables precise measurement of conductivity dependent on CFTR expression. </p>
<h4>Implementation</h4>
- <p></p>
+ <p>We decided to use HEK293T cells lines from Mattijs Bulcaen from KU Leuven [Link] which do overexpress the
+ correct CFTR and those which express CFTR with F508del for the Patch-Clamp measurements. To conduct the
+ Patch-Clamp experiments, we contacted the Cellular Neurophysiology group to perform the necessary
+ measurements. It was a pleasure to work together with Dr. Oliver Dräger, who is working as a post-doc for
+ the Cellular Neurophysiology working group at Bielefeld University. He taught us about the Patch-Clamp
+ method and spent his valuable time supporting our project by guiding our Patch-Clamp measurements. </p>
+ <div className="row align-items-center">
+ <div className="col">
+ <img src="https://static.vecteezy.com/system/resources/previews/016/916/479/original/placeholder-icon-design-free-vector.jpg" style={{width: "30%"}}/>
+ </div>
+ <div className="col">
+ <img src="https://static.vecteezy.com/system/resources/previews/016/916/479/original/placeholder-icon-design-free-vector.jpg" style={{width: "30%"}}/>
+ </div>
+ </div>
+ <p>In summary, through the interview with Prof. Dr. Wischmeyer and the collaboration with his employee
+ Oliver Dräger, we gained valuable insights and optimized our approach to effectively investigate and
+ measure the functionality of the CFTR ion channel, thereby determining the efficiency of our Prime
+ Editing strategy. </p>
+ <h4>References</h4>
+ {/*<!-- Citation num 1--> */}
+<li typeof="schema:ScolarlyArticle" role="doc-biblioentry" property="schema:citation" id="desc-1">
+ <span property="schema:author" typeof="schema:Person">
+ <span property="schema:Name"> Roth, F.</span>;
+ <span property="schema:Name"> Draguhn, A.</span>
+ </span>
+ <span property="schema:name"> Die Entwicklung der Patch-Clamp-Technik. </span>
+ <i property="schema:publisher" typeof="schema:Organization"> Springer eBooks</i>
+ <b property="issueNumber" typeof="PublicationIssue"> </b>,
+ <span property="schema:pageBegin"> 1</span>-<span property="schema:pageEnd">14</span>
+ (<time property="schema:datePublished" datatype="xsd:gYear" dateTime=" 2023">2023</time>).
+ <a className="doi" href="https://doi.org/10.1007/978-3-662-66053-9_1"> doi: 10.1007/978-3-662-66053-9_1</a>
+</li>
+
+{/*<!-- Citation num 2--> */}
+<li typeof="schema:ScolarlyArticle" role="doc-biblioentry" property="schema:citation" id="desc-2">
+ <span property="schema:author" typeof="schema:Person">
+ <span property="schema:Name"> Mete, V.</span>
+ </span>
+ <span property="schema:name"> Entwicklung und Validierung neuer nicht-invasiver Diagnosesysteme für Mucociliary Clearance Disorders (MCCD). </span>
+ <i property="schema:publisher" typeof="schema:Organization"> Dissertation, Westfälische Wilhelms-Universität Münster</i>
+ <b property="issueNumber" typeof="PublicationIssue"> </b>,
+ <span property="schema:pageBegin"> </span>
+ (<time property="schema:datePublished" datatype="xsd:gYear" dateTime=" 2023">2023</time>).
+ <a className="doi" href="https://doi.org/10.17879/98958441905"> doi: 10.17879/98958441905</a>
+</li>
+
+{/*<!-- Citation num 3--> */}
+<li typeof="schema:ScolarlyArticle" role="doc-biblioentry" property="schema:citation" id="desc-3">
+ <span property="schema:author" typeof="schema:Person">
+ <span property="schema:Name"> Giaever, I.</span>;
+ <span property="schema:Name"> Keese, C.</span>
+ </span>
+ <span property="schema:name"> A morphological biosensor for mammalian cells. </span>
+ <i property="schema:publisher" typeof="schema:Organization"> Nature</i>
+ <b property="issueNumber" typeof="PublicationIssue"> 366</b>,
+ <span property="schema:pageBegin"> 591</span>-<span property="schema:pageEnd">592</span>
+ (<time property="schema:datePublished" datatype="xsd:gYear" dateTime=" 1993">1993</time>).
+ <a className="doi" href="https://doi.org/10.1038/366591a0"> doi: 10.1038/366591a0</a>
+</li>
+
+
</>,
cssname: "InvWischmeyer"
},
@@ -227,7 +311,8 @@ let timelinepersontabs =[
Phd
</div>
</div>
- <div className="col">Original language: German</div>
+ <div className="col-3">Original language: German</div>
+ <div className="col"><button>Go to interview</button></div>
</div>
<div className="row">
<div className="col">
@@ -291,7 +376,8 @@ let timelinepersontabs =[
Phd. Student (Molecular Virology and Gene Therapy)
</div>
</div>
- <div className="col">Original language: English</div>
+ <div className="col-3">Original language: English</div>
+ <div className="col"><button>Go to interview</button></div>
</div>
<div className="row">
<div className="col">
@@ -356,7 +442,8 @@ let timelinepersontabs =[
Parent
</div>
</div>
- <div className="col">Original language: German</div>
+ <div className="col-3">Original language: German</div>
+ <div className="col"><button>Go to interview</button></div>
</div>
<div className="row">
<div className="col">
@@ -388,7 +475,8 @@ let timelinepersontabs =[
Parent
</div>
</div>
- <div className="col">Original language: English</div>
+ <div className="col-3">Original language: English</div>
+ <div className="col"><button>Go to interview</button></div>
</div>
<div className="row">
<div className="col">
@@ -421,7 +509,8 @@ let timelinepersontabs =[
Physiotherapist
</div>
</div>
- <div className="col">Original language: German</div>
+ <div className="col-3">Original language: German</div>
+ <div className="col"><button>See interview</button></div>
</div>
<div className="row">
<div className="col">
@@ -439,7 +528,7 @@ let timelinepersontabs =[
<p></p> */}
<h4>Implementation</h4>
<p>The following statement by Katrin Westhoff had a particularly profound impact on our project: "The more we know, the more opportunities we have." We learned from the interview that the current medication is already helping many patients to a huge extent, but that there is still a significant opportunity for improvement. After all, successful gene therapy would markedly enhance the quality of life for those affected. The findings of this project will be disseminated to the relevant researchers in order to facilitate the rapid improvement of the quality of life of all cystic fibrosis patients, regardless of their mutation. </p>
- <button>Jump to visit</button>
+ <button>Jump to visit</button>
</>,
cssname: "InvWesthoff"
},
diff --git a/src/contents/interviews.tsx b/src/contents/interviews.tsx
index 634420b3a2609a1c06e46b20a95dfb5844da246d..c9902efb76c70d9b7d7f3fc36ab840473ab38660 100644
--- a/src/contents/interviews.tsx
+++ b/src/contents/interviews.tsx
@@ -141,7 +141,7 @@ export function Ints() {
<hr/>
<div className="row">
<div className="col-2">
- <div className="t-tag Patient">
+ <div className="t-tag Academia">
Academia
</div>
</div>
@@ -350,6 +350,62 @@ export function Ints() {
a="Answer"
/>
</div>
+ </section>
+ <br/>
+ <section className="col" id="nicole">
+ <h3>Prof. Dr. Erhard Wischmeyer</h3>
+ <hr/>
+ <div className="row">
+ <div className="col-6">
+ <div className="t-tag Academia">
+ Research Group Cellular Neurophysiology, Bielefeld University
+ </div>
+ </div>
+ <div className="col">Original language: German</div>
+ </div>
+ <br/>
+ <div className="row">
+ <div className="col"></div>
+ <div className="col-3">
+ <img className="interview-img" src="https://static.igem.wiki/teams/5247/placeholders/placehilderperson.jpeg"/>
+ </div>
+ </div>
+ <div className="col">
+ <h3>Notes from the interview</h3>
+ <br/>
+ <QaBox
+ q="Can you educate us about your academic career? "
+ a="I did my doctorate 30 years ago at Bielefeld University and then worked at the Max Planck Institute in Göttingen a lot with the Patch-Clamp p technique. Today, I’m head of the working group Cellular Neurophysiology of the medicine faculty of Bielefeld University. "
+ />
+ <QaBox
+ q="What new methods are currently available in electrophysiological research? "
+ a="One of the latest methods is E-cis measurements. These make it possible to examine a monolayer of confluent cells and to measure the membrane potential both above and below. The change in conductivity can be analyzed for instance as a function of CFTR expression. "
+ />
+ <QaBox
+ q="How can we proceed with the investigation of CFTR in different cell cultures by Patch-Clamp? "
+ a="You can study CFTR expression in HEK cells, which allows for a measurable change in chloride conductance. I am not sure whether we will be able to investigate CFTR sufficiently in epithelial cells which you want to collect from your CF patient friend and your team members. That is something we have to try out. "
+ />
+ <QaBox
+ q="How challenging is the measurement of CFTR conductance in epithelial cells? "
+ a="CFTR in epithelial cells has very low conductivity in the femtoampere range. Therefore, extremely sensitive testing is necessary to obtain meaningful results. "
+ />
+ <QaBox
+ q="How challenging is the Patch-Clamp measurement of CFTR conductance in epithelial cells? "
+ a="The project will could take at least one year, even for experienced researchers. "
+ />
+ <QaBox
+ q="What technical challenges do we face in implementing the Patch-Clamp measurements? "
+ a="One of the biggest challenges is measuring the current across the entire cell, as we do not want to carry out single-channel measurements, but rather record the current across cells with a strongly expressing vector carrying the gene for the ion channel. "
+ />
+ <QaBox
+ q="What requirements must be met for cultivation and transfection before the Patch-Clamp measurement? "
+ a="You have to cultivate the cells on poly-lysine and laminin and use round coverslips of 10 mm diameter to prepare them for measurement. For identification of positive transfectants, we use GFP co-transfected cells in our working group, you should think of something like that as well. A transfection rate of 10 % is sufficient to gain enough cells for the measurement. You can think of optimizing your transfection by using Lipofectamin2000 than Lipofectamine2000, which works good for our working group. "
+ />
+ <QaBox
+ q="Who could help us with the Patch-Clamp measurements? "
+ a="The Patch-Clamp devices are heavily utilized in our working group, so you probably cannot perform measurements on your own. However, postdocs could support you for some measurements. Dr. Oliver Dräger is available as a contact person of my working group. "
+ />
+ </div>
</section>
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