diff --git a/src/contents/engineering.tsx b/src/contents/engineering.tsx index c158a0939707ff7e421436b1c0b1fb4486a11e94..a35830aabd3c7c8cd69bb387a9a593e635735fed 100644 --- a/src/contents/engineering.tsx +++ b/src/contents/engineering.tsx @@ -534,12 +534,33 @@ export function Engineering() { </figcaption> </figure> <Collapsible id="Col2" open={false} title="Composition of our SORT LNP called Airbuddy"> - <p>Our lung-specific formulation uses <strong>Selective Organ-Targeting (SORT) LNPs</strong> [1], optimized for stability, targeting, and delivery efficiency. Combined with the <strong>spray-drying technique</strong>, our LNP <strong>AirBuddy</strong> offers an efficient way to deliver mRNA therapeutics to the lungs. The successful delivery of the prime editing complex via inhalation is key to our project.</p> <H4 text="Components of AirBuddy" id="text" /> - <p>erster absatz</p> - <p>zweiter absatz</p> + <H5 text="Ionizable Lipid" id="text" /> + <p>The primary ingredient, 4A3-SC8 or MC3, are ionizable cationic lipids that forms the core of the LNP. Ionizable cationic lipids become positively charged in acidic environments, such as when a pH change occurs for example in acidic buffers or in the endosome. This allows them to bind to negatively charged nucleic acids and form protective capsules around it. In the endosome these lipids facilitate endosomal escape through electrostatic interactions between the LNPs and the endosomal or cellular membranes.</p> + <H5 text="Helper Lipids" id="text" /> + <p>DOTAP (Dioleoyltrimethyl-ammonium propane) is a cationic lipid that makes up 50 % of the total molar lipid ratio. It plays a crucial role in binding to the negatively charged surface of lung epithelial cells. This enhances transfection efficiency and helps make the LNP formulation more lung-specific, improving targeted delivery. The neutral helper lipid DOPE (Dioleoylphosphatidylethanolamine) enhances endosomal escape by fusing with the endosomal membrane and improves transfection efficiency.</p> + <H5 text="Sterol" id="text" /> + <p>Cholesterol, is an important cationic lipid, providing structural stability, fluidity and permeability to the LNPs, thereby improving their overall transfection efficiency. </p> + <H5 text="PEGylated Lipids" id="text" /> + <p>DMG-PEG (Dimyristoylglycerin-polyethyleneglycol) is an important component by improving the LNP stability and preventing aggregation of the LNPs. </p> + <H4 text="Production Methods" id="text" /> + <H5 text="LNP Assembly" id="text" /> + <p>Our LNP can be formulated using various methods depending on the scale of production, including pipette mixing, vortex mixing, or microfluidic mixing. After mixing the lipids with mRNA in carefully controlled ratios, the mixture is typically dialyzed to remove organic solvents like ethanol and citrate buffer. The choice of lipid composition and preparation method influences the tissue-targeting capabilities of the LNPs, allowing for selective delivery to organs like the liver, lungs, or spleen. For more detailed information on formulation methods and lipid selection, refer to our LNP Handbook deigned in cooperation with iGEM Team Linköping and others.</p> + <H5 text="Spray drying Procedure" id="text" /> + <p>By combining these components with the spray drying method from RNhale [2] we offer a versatile and efficient method for delivering mRNA therapeutics to the lung, paving the way for gene therapy, especially our Prime Guide. The effective delivery of the prime editing complex is a crucial point in our project. </p> + <H5 text="Storage" id="text" /> + <p>The final LNP solution can be stored at 4 °C for a few days. It is recommended to use the formulated LNPs as soon as possible to maintain consistent results. Storage at RT is not recommended. Storage at freezing temperatures is also not recommended unless optimized cryoprotectants are used.</p> + </Collapsible> - <p>The final innovation for our LNP to become <strong>AirBuddy</strong> came through consultation with Benjamin Winkeljann from <a onClick={() => goToPagesAndOpenTab('rnhale', '/human-practices')}> RNhale</a>, where the use of spray-drying techniques was discussed. Spray-drying the LNPs, instead of using traditional methods, helped improve stability and eco-friendliness of the product [8]. The spray-dried SORT LNPs demonstrated lower cytotoxicity, and the technique proved effective in maintaining particle integrity.</p> + <p>The final innovation for our LNP to become <strong>AirBuddy</strong> came through consultation with Benjamin Winkeljann from <a onClick={() => goToPagesAndOpenTab('rnhale', '/human-practices')}> RNhale</a>, where the use of spray-drying techniques was discussed. Spray-drying the LNPs, instead of using traditional methods, helped improve stability and eco-friendliness of the product [8]. The spray-dried SORT LNPs demonstrated lower cytotoxicity, and the technique proved effective in maintaining particle integrity. In conclusion, we created a stable LNP for efficient delivery of mRNA therapeutics to the lungs since the successful delivery of the prime editing complex via inhalation is key to our project. </p> + <figure> + <img src="https://static.igem.wiki/teams/5247/delivery/big-plan-inhalation-teil-del.webp" alt="Flow DEL"/> + <figcaption> + <b>Figure. </b> + Application stategy - AirBuddy is inhaled by the patient, enabling uptake of PrimeGuide in lung epithelial cells via endocytosis. + </figcaption> + </figure> + </p> </div> <div className="box" > diff --git a/src/contents/safety.tsx b/src/contents/safety.tsx index a938fb170444e7e75a3ae7654c340395a565d419..ea3760061a350690631986ec112119e4152d2041 100644 --- a/src/contents/safety.tsx +++ b/src/contents/safety.tsx @@ -134,14 +134,20 @@ export const Safety: React.FC = () =>{ <p> In our cell culture laboratory, we work under sterile conditions to ensure optimal growth conditions for human cell lines. Among other things, we carry out transfections in order to introduce genetic material into cells and investigate their behavior. Strict protocols and state-of-the-art technology ensure the precision and reproducibility of our experiments. </p> + <div className="figure-wrapper"> + <figure> + <img src="https://static.igem.wiki/teams/5247/photos/biosafety/kollage/new/img-2040.jpeg" style={{height: "10%"}}/> + <figcaption> <b>Photo-gallery of S1 laboratory. A: Safety cabinets. B: Incubator. C: Safety cabinet.</b> </figcaption> + </figure> + </div> <p> In our S2 laboratory, the harvested nasal epithelial cells that serve as primary cultures undergo a comprehensive HHH test (link zu primär Kulturen) to ensure their safety and suitability for further experiments. This test is crucial to ensure that we can subsequently work safely with these cells in the S1 range without the risk of contamination or unwanted release of biological material. </p> <div className="figure-wrapper"> <figure> - <img src="https://static.igem.wiki/teams/5247/photos/biosafety/s2/s2-lab.jpeg" style={{height: "10%"}}/> - <figcaption> <b>Figure x.</b> </figcaption> + <img src="https://static.igem.wiki/teams/5247/photos/biosafety/kollage/new/img-2042.jpeg" style={{height: "10%"}}/> + <figcaption> <b>Photo-gallery of S2 laboratory. A: Door of S2 lab with S2 sign. B: Emergency shower and fire distinguisher. C: Clean bench with centrifuge. D: Incubator. E: Safety cabinet. F: Emergeny telephone. G: S2 lab coat with S2 sign. H: Microscope. I: Autoclave.</b> </figcaption> </figure> </div> </Section> diff --git a/src/data/hptimelinedata.tsx b/src/data/hptimelinedata.tsx index b499542361668d9475cca85fc61294240c60cca1..6a4e6424860790dc49082dcc22cce509d7f9074f 100644 --- a/src/data/hptimelinedata.tsx +++ b/src/data/hptimelinedata.tsx @@ -1120,7 +1120,7 @@ export const timelinedata: Array<TimelineDatenpunkt> = [ interviewtabid: "corden", cardtext: "", language: "de", - quote: "", + quote: "X", aimofcontact: [<p>The goal of the discussion with Steffen Bira and Serra Gürcan from Corden Pharma was to gain insight into the design, stability, and practical application of lipid nanoparticles (LNPs) for use in gene therapy. The conversation focused on the possibility of using Corden Pharma’s LNP starter kits, understanding the factors affecting the stability of LNPs, and exploring options for incorporating antibodies into LNPs to target specific cells. </p>], insights: [<p>Steffen Bira and Serra Gürcan of Corden Pharma[Link Corden Pharmahttps://cordenpharma.com/] indicated that our planned spray drying approach of LNPs has not been extensively explored within their operations. The company focuses primarily on the aseptic fill and finish of LNPs, particularly for injectable formulations. However, they acknowledged the potential for spray drying and recommended consulting with specialized companies to assess the feasibility. The stability of the lipids used in LNPs during the spray drying process was also identified as an area requiring further investigation. @@ -1206,6 +1206,7 @@ export const timelinedata: Array<TimelineDatenpunkt> = [ aimofcontact: [<p>We contacted the university because of the urgent need to address the issue of hygiene for students and staff, particularly those with immunocomprised students and staff. There was a need to develop an effective hygiene concept to ensure the health and safety of these people. We developed this concept in collaboration with Max, our CF friend. [Link] </p>], insights: [<p>We learnt that our hygiene concept is very well-developed. But although a well-developed hygiene concept is already existing, strategic development and a step-by-step approach are needed. In particular, the step-by-step implementation was emphasized, like starting with equipping the toilets. Bureaucratic hurdles, such as the need to apply to the rectorate, were identified as a major challenge. In addition, it became clear that there is a great need for sanitary facilities and facilities for the disabled, especially due to the needs of students and staff with health problems. Interaction and networking with other universities was also considered valuable. </p>], implementation: [<p>The next phase of developing a new hygiene concept is to maintain contact with Mr. Johannfunke in order to continue to advance the hygiene concept in collaboration. The strategic approach entails the incremental implementation of measures, exemplified by the establishment of the inaugural toilet facility within the main building. It is of the utmost importance to ensure the uninterrupted implementation of the hygiene concept. In order to achieve this, it is essential to draw upon the existing plans and measures that have already been implemented in new buildings. We are working on advancing the plans at a higher level and are in regular dialogue with the Central contact point Barrier-free in order to overcome bureaucratic hurdles and actively promote the topic. Furthermore, it is necessary to intensify lobbying work in order to gain greater support for this issue at both the university and political levels. </p>], + language: "de", interview: <> <QaBox q="What do you think of our hygiene concept and our plan?" a="This is a very acute problem. It particularly affects students with disabilities and immune-compromised staff, such as those with cancer or cystic fibrosis, who are forced to work from home. The problem is: Employees can work from home, but students cannot. There is a great need for hygiene measures, as contact must be avoided to minimise the risk of infection."/> <QaBox q="What are the challenges in implementing the hygiene concept?" a="There is a lack of strategic development, although your hygiene concept is well developed. It is necessary to proceed in small steps, e.g. starting with a toilet in the main building. However, bureaucracy is a major obstacle. To be implemented, an application has to be submitted to the rectorate, and these processes are often lengthy and complicated."/> @@ -1249,6 +1250,7 @@ export const timelinedata: Array<TimelineDatenpunkt> = [ aimofcontact: [<p>The aim of our contact with Philipp Kühnel, a PhD student from the Otorhinolaryngology working group of Bielefeld University, was to gain expertise in working with primary cultures, particularly focusing on air-liquid interface (ALI) cultures. Given his experience in this area, we sought his guidance to ensure that we were following best practices and to address any technical challenges we might encounter.</p>], insights: [<p>Through our discussions with Philipp, we gained valuable insights into the optimal conditions for cultivating primary cells and maintaining ALI cultures. He provided practical advice on troubleshooting of common issues, such as cell differentiation and culture stability, which were crucial for the success of our experiments. We also maintained close contact to exchange information about fungi that frequently contaminate ALI cultures. The expertise shared on combating these fungal contaminations was particularly valuable and greatly enhanced our understanding of effective prevention and treatment methods. </p>], implementation: [<p>We incorporated Philipp’s advice by refining our culture protocols, particularly adjusting the conditions for ALI cultures to improve cell differentiation and overall culture health. This directly enhanced the reliability of our experimental results, ensuring that our work with primary cultures was both accurate and reproducible. </p>], + language:"de", summary: "The contact aimed to leverage Philipp’s expertise in ALI cultures to improve our experimental protocols Gained insights into optimizing conditions for primary cell cultures and managing common challenges like fungal contamination" }, { @@ -1267,6 +1269,7 @@ export const timelinedata: Array<TimelineDatenpunkt> = [ insights: "We were provided with invaluable insights into the quality and project management of the biobank and storage of patient samples. It was of particular interest to note that Biobank OWL occupies a distinctive position in this context, insofar as a trustee is not a mandatory figure within its system and is therefore not provided for as a standard component. However, Biobank OWL has elected to integrate a trustee in order to enhance the security standards for the safeguarding of patient data. This illustrates the biobank's dedication to ensuring the optimal protection and security of sensitive patient data.", implementation: "The insights gained have facilitated a deeper comprehension of the significance of quality management in the processing of patient samples. This understanding has been integrated into our project processes, thereby enhancing the accuracy and reliability of our procedures. ", summary: "The interview focused on understanding the operations of the Biobank OWL, particularly in the areas of quality management and sample processing. Provided a detailed overview of biobank activities, including sample collection, storage conditions, and data protection measures", + language: "de", interview: <> <QaBox q="Can you briefly explain to us what exactly a biobank is and what its main tasks are?" a="A biobank is a specialized facility that collects, stores, and manages biological samples and associated data for research purposes. Each biobank is unique in its operations and functions. In Bielefeld and Lippe, the Biobank BOWL (Biobank OWL) is responsible for the storage of patient samples. The Data Integration Centre (DIZ) stores data pertaining to these samples. A trustee oversees the pseudonymisation of data, acting as an interface between BOWL and DIZ, ensuring that patient data cannot be directly linked to patient samples." />