From 5bd8ce9b63c7f0e99727456c175fab1c31205938 Mon Sep 17 00:00:00 2001
From: Jincheng Lyu <1254883431@qq.com>
Date: Thu, 14 Dec 2023 07:59:37 +0000
Subject: [PATCH] Update results.html
---
wiki/pages/results.html | 67 +++++++++++++++++++----------------------
1 file changed, 31 insertions(+), 36 deletions(-)
diff --git a/wiki/pages/results.html b/wiki/pages/results.html
index 335d6cd..2e53ae9 100644
--- a/wiki/pages/results.html
+++ b/wiki/pages/results.html
@@ -121,9 +121,6 @@
<h3 class="myPage-paragraph-headline-h3" id="childSection1"><b>Highlight</b></h3>
<ul class="myPage-paragraph-ul">
<li>Confirm the secretion function of the signal peptide KpSP and LMT</li>
- <li>Construct a well-functioning transcription system based on the cold-adapted VSW-3 RNAP and
- its cognate
- promoter
</li>
<li>Improve the growth ability and protein expression ability of bacteria at low temperature
</li>
@@ -146,9 +143,7 @@
verified the
secretion function of the two signal peptides KpSP and LMT. Second, we combine a logic AND gate
with the
- CspA CRE to alleviate oxidative stress in low temperatures. Third, we design another
- low-temperature
- response AND gate expression system to weaken the leakage expression based on VSW-3 RNAP. At
+ CspA CRE to alleviate oxidative stress in low temperatures. At
last, we use
MV<sup>140</sup> to display the CBM on the surface of engineered <i>E. coli</i> to adsorb
cellulose, which prevents the
@@ -702,7 +697,7 @@
ability of bacteria at 4 °C, characterized the CspA CREC system and constructed AND-logic gate
based
on
- <i>hrp</i> or the novel VSW-3 RNAP to reduce the leakage of CspA CREC system. And for the second
+ <i>hrp</i> to reduce the leakage of CspA CREC system. And for the second
theme,
we demonstrated the function both of signal peptide Kp-SP and LMT to direct GFP to the
extracellular
@@ -873,7 +868,7 @@
</div>
<p class="myPage-paragraph-content">
- As shown in Fig. 23, the concentration of hyaluronic acid produced by our engineered strain was
+ As shown in Fig. 22, the concentration of hyaluronic acid produced by our engineered strain was
higher
than
that of the control group. It demonstrated that the engineered strain was successfully
@@ -985,7 +980,7 @@
</div>
</div>
<p class="myPage-paragraph-content">
- As shown in Fig. 27, the concentration of bacterial cellulose by our engineered strain was
+ As shown in Fig. 26, the concentration of bacterial cellulose by our engineered strain was
higher
than
that
@@ -1169,7 +1164,7 @@
regulation. The determination of OD<sub>600</sub>-t and fluorescence-t showed the
growth of EcNP. At the same time, we observed that when OD<sub>600</sub>>2, the
fluorescence of EcNP expression showed a linear relationship with its OD<sub>600</sub>
- value (R<sup>2</sup>=0.9727) (Fig 32), which was consistent with the results in the
+ value (R<sup>2</sup>=0.9727) (Fig 33), which was consistent with the results in the
references (2). This means that when the OD<sub>600</sub> value of bacteria is higher
than 2, we can calculate the bacterial population density of EcNP in the mixed bacterial
broth through the fluorescence intensity measured, which provides strong support for the
@@ -1434,11 +1429,11 @@
, mazF (<a href=" http://parts.igem.org/Part:BBa_ K1096002 "><b>BBa_ K1096002</b></a>)
to construct the composite part BBa_K3332081. </p>
- <!---Fig. biosafety 1--->
+ <!---Fig. biosafety 42--->
<div class="myPage-paragraph-fig">
<img src=" https://static.igem.wiki/teams/4907/wiki/result/fig-56.png" alt=""
- style="max-width:100%">
- <div class="myPage-paragraph-fig-description"><b> Fig. 1 CFU assay for characterizing
+ style="max-width:50%">
+ <div class="myPage-paragraph-fig-description"><b> Fig. 42 CFU assay for characterizing
the
killing
effect
@@ -1457,7 +1452,7 @@
decreased significantly after 8 hours. And there was almost no bacterial growth in 15
hours
(Fig.
- 1),
+ 42),
which could verify that BBa_K3332081 has the killing function without L-arabinose. In
conclusion,
This
@@ -1489,11 +1484,11 @@
composite part BBa_K3332083, which were assembled on pSB1C3 backbone by standard
assembly. </p>
- <!---Fig. biosafety 2--->
+ <!---Fig. biosafety 43--->
<div class="myPage-paragraph-fig">
<img src=" https://static.igem.wiki/teams/4907/wiki/result/fig-57.png" alt=""
- style="max-width:100%">
- <div class="myPage-paragraph-fig-description"><b> Fig. 2 Survival Ratio of different
+ style="max-width:50%">
+ <div class="myPage-paragraph-fig-description"><b> Fig. 43 Survival Ratio of different
groups
against
time
@@ -1505,7 +1500,7 @@
CFU assay was implemented to characterize the effect of toxin MazF. We observed that the
number
of
- colonies on the plate decreased significantly after induction (Fig. 2). This
+ colonies on the plate decreased significantly after induction (Fig. 43). This
demonstrated that
toxin
MazF could be lethal to the bacteria and the kill switch (<a
@@ -1559,13 +1554,13 @@
transformed into <i>E. coli</i>DB3.1, followed by positive transformant selection using
kanamycin
and
- confirmation through colony PCR (Fig. 50) and sequencing. </p>
+ confirmation through colony PCR (Fig. 44) and sequencing. </p>
<!---Fig. biosafety 3--->
<div class="myPage-paragraph-fig">
<img src=" https://static.igem.wiki/teams/4907/wiki/result/fig-50.png" alt=""
- style="max-width:100%">
- <div class="myPage-paragraph-fig-description"><b> Fig. 50 DNA gel electrophoresis of the
+ style="max-width:50%">
+ <div class="myPage-paragraph-fig-description"><b> Fig. 44 DNA gel electrophoresis of the
colony
PCR
products of BBa_K4907139.</b>
@@ -1582,13 +1577,13 @@
was
transformed into <i>E. coli</i>DH10β, followed by positive transformant selection using
chloramphenicol
- and confirmation through colony PCR (Fig. 51) and sequencing. </p>
+ and confirmation through colony PCR (Fig. 45) and sequencing. </p>
<!---Fig. biosafety 4--->
<div class="myPage-paragraph-fig">
<img src=" https://static.igem.wiki/teams/4907/wiki/result/fig-51.png" alt=""
- style="max-width:100%">
- <div class="myPage-paragraph-fig-description"><b> Fig. 51 DNA gel electrophoresis of the
+ style="max-width:50%">
+ <div class="myPage-paragraph-fig-description"><b> Fig. 45 DNA gel electrophoresis of the
colony
PCR
products of BBa_K4907138.</b>
@@ -1683,13 +1678,13 @@
transformed into<i>E.
coli</i>DH10β, followed by positive transformant selection using kanamycin through
colony PCR
- (Fig. 52) and sequencing. </p>
+ (Fig. 46) and sequencing. </p>
<!---Fig. biosafety 5--->
<div class="myPage-paragraph-fig">
<img src=" https://static.igem.wiki/teams/4907/wiki/result/fig-52.png" alt=""
- style="max-width:100%">
- <div class="myPage-paragraph-fig-description"><b> Fig. 52 DNA gel electrophoresis of the
+ style="max-width:50%">
+ <div class="myPage-paragraph-fig-description"><b> Fig. 46 DNA gel electrophoresis of the
colony
PCR products of BBa_K4907131.</b>
</div>
@@ -1769,14 +1764,14 @@
assembly. This
constructed circuit was transformed into <i>E. coli</i>DH10β, followed by positive
transformant
- selection using kanamycin and confirmation through colony PCR (Fig. 53) and sequencing.
+ selection using kanamycin and confirmation through colony PCR (Fig. 47) and sequencing.
</p>
<!---Fig. biosafety 6--->
<div class="myPage-paragraph-fig">
<img src=" https://static.igem.wiki/teams/4907/wiki/result/fig-53.png" alt=""
- style="max-width:100%">
- <div class="myPage-paragraph-fig-description"><b> Fig. 53 DNA gel electrophoresis of the
+ style="max-width:50%">
+ <div class="myPage-paragraph-fig-description"><b> Fig. 47 DNA gel electrophoresis of the
colony
PCR products (BBa_K4907140).</b>
</div>
@@ -1796,8 +1791,8 @@
<!---Fig. biosafety 7--->
<div class="myPage-paragraph-fig">
<img src=" https://static.igem.wiki/teams/4907/wiki/result/fig-54.png" alt=""
- style="max-width:100%">
- <div class="myPage-paragraph-fig-description"><b> Fig. 54 The function of BBa_I0500 was
+ style="max-width:50%">
+ <div class="myPage-paragraph-fig-description"><b> Fig. 48 The function of BBa_I0500 was
characterized by survival. a: OD<sub>600</sub> values of bacteria upon addition of
L-arabinose (left) and glucose (right). b: CFU values of bacteria upon addition of
glucose
@@ -1823,8 +1818,8 @@
<!---Fig. biosafety 8--->
<div class="myPage-paragraph-fig">
<img src=" https://static.igem.wiki/teams/4907/wiki/result/fig-55.png" alt=""
- style="max-width:100%">
- <div class="myPage-paragraph-fig-description"><b> Fig. 55 Growth curve and survival
+ style="max-width:50%">
+ <div class="myPage-paragraph-fig-description"><b> Fig. 49 Growth curve and survival
assay for
characterizing the function of <i>ccdB</i>. a The value of OD<sub>600</sub> against
time (h)
@@ -1841,13 +1836,13 @@
<i>ccdB</i>
expressed grew rapidly, while the one expressing <i>ccdB</i> showed a significant growth
defect,
- as the optical density (at 600 nm) increased very slightly (Fig. 55a).
+ as the optical density (at 600 nm) increased very slightly (Fig. 49a).
</p>
<p class="myPage-paragraph-content">
At each time, the spot assay was also performed, then the cell viability was measured by
CFU
- count (Fig. 55b). Consistent with the trend of OD<sub>600</sub> value against time, only
+ count (Fig. 49b). Consistent with the trend of OD<sub>600</sub> value against time, only
the
absence of <i>ccdB</i> allowed the host cells to survive. All these results indicated
that <i>ccdB</i>
--
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