{% extends "layout.html" %} {% block title %}Contribution{% endblock %} {% block lead %}Make a useful contribution for future iGEM teams. Use this page to document that contribution.{% endblock %} {% block page_content %}
Make a useful contribution for future iGEM teams. Use this page to document that contribution.
If you are making a contribution by adding information to an existing Part or creating a new Part, you must document your contribution on the Part's Main Page on the Registry for your team to be eligible for this criteria. You can use this page to link to that part and include additional information about your contribution.
Please see the 2023 Medals Page for more information.
The software contribution of the project offers a complement to the Stealth program in the form of a software pipeline titled Chameleon that takes the input of a synthetic plasmid sequence and outputs a codon-optimized and Stealth motif removed version of the same plasmid sequence. Chameleon aims to accomplish: reading in plasmid sequence and target host genome, performing Stealth analysis to identify target motifs, performing codon frequency analysis for codon optimization, and finally outputting a Stealth motif reduced plasmid that preserves the desired functionality while improving transformation efficiency.
The specific strain of M. aeruginosa, UTEX 2385, has not had a ribosome binding site (RBS) identified prior to this project. The putative M. aeruginosa RBS was identified bioinformatically from nucleotide sequences directly upstream of the M. aeruginosa PCC 7806 ribosome genes, and verified by the 16S rRNA anti-RBS sequence. Here we contribute an RBS for cyanobacteria engineering to the iGEM community.