
Coding
Part:BBa_K916000
Designed by: Joseph Elsherbini Group: iGEM12_Georgia_Tech (2012-09-26)
TraR Coding Sequence
TraR Coding Sequence from Agrobacterium tumefaciens
NOTE: This part has no promoter, and so can be used with any BioBrick promoter in the registry. See the list of promoters here.
Usage and Biology
3-oxo-C8-HSL Figure 1 Luo ZQ, Su S, Farrand SK. In situ activation of the quorum-sensing transcription factor TraR by cognate and noncognate acyl-homoserine lactone ligands: kinetics and consequences. J Bacteriol. 2003 Oct;185(19):5665-72. PMID: 13129937 http://www.ncbi.nlm.nih.gov/pubmed/13129937
TraR is the A. tumefaciens transcriptional regulator responsible for its quorum sensing response. It binds N-(3-Oxo-octanoyl)-l-homoserine lactone (3-oxo-C8-HSL), and upon binding forms a homodimer. Most similar LuxR family quorum sensing regulators dimerize without their cognate autoinducer, but TraR will only dimerize upon binding 3-oxo-C8-HSL.
However, it has been shown that TraR cannot activate transcription in E. coli without adding other accessory factors from Agrobacterium. SOURCE.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |