
Part:BBa_K200028
Protease resistant PAH
The Homo sapiens endogenous enzyme is synthesised by the liver. However, as part of the Imperial College iGEM 2009 project, The E.ncapsulator, the enzyme is released in the intestine of the individual. The presence of proteases in the intestine was therefore a serious problem which would jeopardise the viability of the curative enzyme. We thus performed site directed mutagenesis in order to alter a serine residue (Ser16) to a glutamine. This change has previously been correlated with resistance against intestinal proteases#PRPAH1. In fact, it is thought that phosphorylation of the serine 16 is responsible for the resistance to proteases#PRPAH2. This phosphorylated state is mimicked by replacing the serine with a glutamine residue.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 287
Illegal BamHI site found at 814
Illegal XhoI site found at 524 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
<biblio>
- PRPAH1 pmid=7887915
- PRPAH2 pmid=8573072
</biblio>
None |