
Part:BBa_K1218007
HisC E. coli (Ancestral)
HisC is a gene that makes a phosphate aminotransferase essential to the production of histidine. Histidine does not grow abiotically, so this part was characterized in a pUC19 expression vector by rescuing HisC knockout strains grown on M9 minimal plates using IPTG as an inducer.
We gathered data on extant HisC genes using the seed sequences from the PFAM data base to create a phylogenetic tree using PhyML. We then used ProtTest to determine that the best ancestral reconstruction model was the WAG model, which we performed using PAML with Lazarus. See the Stanford-Brown 2013 iGEM wiki for process details and authenticity. The resulting phosphate aminotransferase is 39.1% similar to the extant protein in E. coli.
Usage and Biology

Negative HisC control plated on M9 minimal media (Exhibits few misshaped colonies)
Positive Control HisC plated on M9 minimal media (Exhibits many regularly spaced, healthy colonies)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 433
- 1000COMPATIBLE WITH RFC[1000]
None |