
Coding
FtsZ
Part:BBa_K1011000
Designed by: VGEM 2013 Group: iGEM13_Virginia (2013-09-12)
ftsZ gene from E. coli on pSB1C3
This is an E. coli ftsZ gene. FtsZ is a protein that polymerizes into the Z ring that is formed at the septum site during cellular division. The over expression of ftsZ will lead to non-specific septum sites at the polar ends, which result in the production of E. coli minicells.
As this picture illustrates, under normal WT conditions, FtsZ will form the Z ring at the middle of the cell. When overexpressed, FtsZ will polymerize non specifically at the polar ends of the cell, thereby cleaving the cell to form a minicell.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 322
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
None |