Coding

Part:BBa_K3134007

Designed by: Shiyuan Li   Group: iGEM19_Nanjing_High_School   (2019-10-16)


YF1

YF1 is especially sensitive to blue light. With FixJ, it can record the light. In the absence of blue light, it phosphorylates its cognate response regulator FixJ, which then drives robust gene expression from the FixK2 promoter. In the presence of blue light, however, net kinase activity of YF1 and consequent gene expression are greatly reduced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 544
    Illegal NgoMIV site found at 616
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 724
    Illegal AgeI site found at 258
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 157


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