
Coding
CapH
Part:BBa_K314015
Designed by: Chris Eiben, Tomas Huber, David Zong Group: iGEM10_Washington (2010-10-08)
CapD_CP-F24H
A mutated CapD_CP protein aimed at increasing hydrolysis and decreasing transpeptidation of PDG.
Usage and Biology
To test BBa _K314015, it was inserted into a pET29b+ vector using Kunkel Mutagenesis. CapD_CP-F24H was then produced and purified as described in the UW 2010 iGEM Team's Small Scale Protein Purification Protocol. The purified protein was then tested for activity against CapD_CP. For a detailed description of the assay, please see the 2010 UW iGEM wiki. The resulting data is shown below.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1489
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |