Coding

Part:BBa_K200028

Designed by: Charles Fracchia, Royah Vaezi   Group: iGEM09_Imperial College London   (2009-10-15)

Protease resistant PAH

The Homo sapiens endogenous enzyme is synthesised by the liver. However, as part of the Imperial College iGEM 2009 project, The E.ncapsulator, the enzyme is released in the intestine of the individual. The presence of proteases in the intestine was therefore a serious problem which would jeopardise the viability of the curative enzyme. We thus performed site directed mutagenesis in order to alter a serine residue (Ser16) to a glutamine. This change has previously been correlated with resistance against intestinal proteases#PRPAH1. In fact, it is thought that phosphorylation of the serine 16 is responsible for the resistance to proteases#PRPAH2. This phosphorylated state is mimicked by replacing the serine with a glutamine residue.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


<biblio>

  1. PRPAH1 pmid=7887915
  2. PRPAH2 pmid=8573072

</biblio>

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Categories
Parameters
None