
Part:BBa_K2881000
mMBD-eGFP-avi-His
MBD stands for Methyl-CpG-binding proteins. This family of proteins contain MBD domains specifically binding to methylated CpG sites in vitro. Our part aims to utilize MBD domain to quantify methylation levels of circulating tumor DNA (ctDNA); it is also useful for characterizing other methylated DNA substrates. A single mMBD domain is fused to eGFP to provide a way to visualize the signal; eGFP also significantly enhances expression of this part in BL-21(DE3). His-tag enables the protein to be purified by Nickel column. Avi-tag serves as a versatile platform for other signal detection/amplification possibilities: it could be specifically recognized by BirA enzyme in vitro to attach a biotin.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 201
Illegal BamHI site found at 1287 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2080
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