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CapH

Part:BBa_K314015

Designed by: Chris Eiben, Tomas Huber, David Zong   Group: iGEM10_Washington   (2010-10-08)

CapD_CP-F24H

A mutated CapD_CP protein aimed at increasing hydrolysis and decreasing transpeptidation of PDG.

Usage and Biology

To test BBa _K314015, it was inserted into a pET29b+ vector using Kunkel Mutagenesis. CapD_CP-F24H was then produced and purified as described in the UW 2010 iGEM Team's Small Scale Protein Purification Protocol. The purified protein was then tested for activity against CapD_CP. For a detailed description of the assay, please see the 2010 UW iGEM wiki. The resulting data is shown below.

Kinetic constants, kcat and Km, taken from our plotted curves are shown in the table above.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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