
Part:BBa_K909009
cDNA of UV-B sensing protein UVR8 from Arabidopsis thaliana
UVR8 (UV resistance locus 8) is UV-B photoreceptor found in plants as a crucial mediator of plants response to UV-B response. In absence of UV-B the protein occurs as a dimer, which breaks upon UV-B irradiation.
Usage and Biology
Differently from other photoreceptors, UVR8 uses tryptophane residues present in the dimer interfase, rather than external chromophores. Energy from UV-B light is absorbed by these triptophanes and later used to break dimer cross-salt bridges resulting in the dissociation of the UVR8 monomers (Christie 2012). Monomeric UVR8 is then able to initiate downstream reactions that trigger the transcription of proteins needed for the UV-B caused stress response (Heijde 2012).
We used UVR8 to create a novel UV-B switch in E.coli, by fusing it with TetR DNA binding domain (BBa_K909008). Additionally we inserted BamHI site after ATG codon for similar protein-protein fusions with this biobrick.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 560
Illegal PstI site found at 1214 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 76
Illegal PstI site found at 560
Illegal PstI site found at 1214 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 560
Illegal PstI site found at 1214 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 560
Illegal PstI site found at 1214 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 91
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