
Coding
lasI
Part:BBa_C0078
Designed by: Chris Walsh (Fighting Darwins) Group: Antiquity (2004-01-27)
autoinducer synthetase for PAI from Pseudomonas aeruginosa
coding region for lasI protein, which produces the chemical signal AI-1
Usage and Biology
214 c->t EcoRI restriction site was present. Ttc -> ttt codon (good for E. coli)
402 g->a PstI restriction site was present. Cag -> Caa codon (slightly less optimal for E. Coli)
Sequence and Features
Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 661
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 223
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//function/biosynthesis/ahl
//function/cellsignalling
//function/cellsignalling
direction | Forward |
ec_num | none |
kegg | pae:PA1432 |
protein | lasI |
swisspro | P33883 |
tag | LVA |