
Part:BBa_K771103
ssDsbA-PDZdomain
Component of interacting proteins of Membrane Anchor/Rudder system.
Usage and Biology
The PDZ domain along with its ligand(Part:BBa_K771104) comes from Mus musculus, which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by 2012 SJTU-BioX-Shanghai Team, the PDZ domain is located in Membrane Anchor 4, while the PDZ ligands on Membrane Anchor 3. Due to the fact that the PDZ domain and ligand is in the N-terminus of the whole protein, we added a SRP-dependent signal peptide, ssdsbA, to help the Membrane Anchor localize to the membrane correctly.
Related Parts
Part:BBa_K771003, Membrane Anchor 3, containing PDZ ligand
Part:BBa_K771004, Membrane Anchor 4, containing PDZ domaim
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 115
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 345
n/a | ssDsbA-PDZdomain |