
Coding
Part:BBa_K3981005
Designed by: Yulin Peng Group: iGEM21_AHUT_China (2021-08-27)
Vgb
Vireoscilla hemoglobin (VHb) of Gram-negative aerobic bacterium vireoscilla is the first bacterial hemoglobin found. Its function is to isolate oxygen, especially when it is at low concentration, and transport it to terminal respiratory oxidase, so as to enhance oxidative phosphorylation. Vitreoscilla hemoglobin gene (vgb) was introduced into E. coli, cell growth and target protein production increased significantly. The vgb promoter is regulated by oxygen at the transcriptional level, and hypoventilation is sufficient to induce VHb gene expression.[2] We used vgb promoter to replace the T7 promoter of pET-28A (+) to enable E. coli Nissle 1917 (ECN) to transcribe under hypoxic conditions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |