Coding
LacI

Part:BBa_I732100

Designed by: Zhan Jian   Group: iGEM07_USTC   (2007-10-14)

LacI

Figure 1 Solution structure of a dimer of Lac repressor DNA-binding domain complexed to its natural operator O1 (From RCSB)

The sequence produce LacI repressor protein.Standard BioBrick Prefix and Suffix are added to both ends of this part for standard assembly.


Usage and Biology

The lac repressor is a DNA-binding protein which inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria. There are three distinct regions in the protein. The headpiece, a fragment contains approximately 51 amino acids from its N-terminal together with a helix-turn-helix motif, is the only region that serves to bind DNA. Figure I shows the NMR structure of the complex with two headpieces of the lac repressor and its nature operator.

(Reference: 1.Lewis, M. (2005), 'The lac repressor.', C R Biol 328 (6), 521--548. 2.Lehming, N.; Sartorius, J.; Oehler, S.; von Wilcken-Bergmann, B. & Müller-Hill, B. Recognition helices of lac and lambda repressor are oriented in opposite directions and recognize similar DNA sequences. PNAS, 1988, 85, 7947-7951 3. Sartorius, J.; Lehming, N.; Kisters, B.; von Wilcken-Bergmann, B. & Müller-Hill, B. (1989), 'lac repressor mutants with double or triple exchanges in the recognition helix bind specifically to lac operator variants with multiple exchanges.', EMBO J 8(4), 1265--1270. )




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/repressor
Parameters
None