Coding

Part:BBa_K909009

Designed by: Gintautas Vainorius   Group: iGEM12_ETH_Zurich   (2012-09-23)

cDNA of UV-B sensing protein UVR8 from Arabidopsis thaliana

UVR8 as a symmetric homodimer. Upon UV-B exposure the the dimer dissociates into two monomers. (Heijde 2012)

UVR8 (UV resistance locus 8) is UV-B photoreceptor found in plants as a crucial mediator of plants response to UV-B response. In absence of UV-B the protein occurs as a dimer, which breaks upon UV-B irradiation.

Usage and Biology

Differently from other photoreceptors, UVR8 uses tryptophane residues present in the dimer interfase, rather than external chromophores. Energy from UV-B light is absorbed by these triptophanes and later used to break dimer cross-salt bridges resulting in the dissociation of the UVR8 monomers (Christie 2012). Monomeric UVR8 is then able to initiate downstream reactions that trigger the transcription of proteins needed for the UV-B caused stress response (Heijde 2012).

We used UVR8 to create a novel UV-B switch in E.coli, by fusing it with TetR DNA binding domain (BBa_K909008). Additionally we inserted BamHI site after ATG codon for similar protein-protein fusions with this biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 76
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 91


[edit]
Categories
//cds/receptor
Parameters
None