
Coding
PIF-6
Part:BBa_K365001
Designed by: RENAULT Renaud Group: iGEM10_ESBS-Strasbourg (2010-09-14)
Phytochrome Interacting Factor 6 (aa 1-100)
First hundred aminoacids of Phytochrome Interacting Factor 6 (PIF6) from A. thaliana, which physically interacts with Phytochrome B.
Background:
For the design of the first engineered system that achieved to enable the spatiotemporal control of PhyB-PIF interactions in in-vivo experiments, (Lim & Voigt, 2009.) screened multiple potential phytochrome–PIF pairs by a fluorescence translocation assay in NIH3T3 cells. They measured the red-light-induced translocation of yellow fluorescent protein (YFP) fused to PIF domains to co-expressed phytochrome domains fused through a flexible linker to mCherry and localized to the plasma membrane by a carboxyterminalpolybasic, prenylation sequence from Kras. Of all previously reported PIF domains, only the N terminus of PIF6 is strong enough to cause significant translocation of YFP to the membrane.
pairs in a fluorescence translocation assay.

The stucture below is modeled by homology using the server I-TASSER
You can visit our wiki "visual description" page for more info
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Conception:
We chose used the same sequence of the last 100-residue N-terminal activated phytochrome binding (APB) domain of PIF6, which was already successfully used by (Lim & Voigt, 2009.).
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Categories
Parameters
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