Coding
FtsZ

Part:BBa_K1011000

Designed by: VGEM 2013   Group: iGEM13_Virginia   (2013-09-12)

ftsZ gene from E. coli on pSB1C3

This is an E. coli ftsZ gene. FtsZ is a protein that polymerizes into the Z ring that is formed at the septum site during cellular division. The over expression of ftsZ will lead to non-specific septum sites at the polar ends, which result in the production of E. coli minicells.

As this picture illustrates, under normal WT conditions, FtsZ will form the Z ring at the middle of the cell. When overexpressed, FtsZ will polymerize non specifically at the polar ends of the cell, thereby cleaving the cell to form a minicell.

FtsZ Comparison.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 322
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None