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Coding

Part:BBa_K771103

Designed by: Guo Huaqing   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-23)

ssDsbA-PDZdomain

Component of interacting proteins of Membrane Anchor/Rudder system.


Usage and Biology

12SJTU 2PDZ.gif

The PDZ domain along with its ligand(Part:BBa_K771104) comes from Mus musculus, which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by 2012 SJTU-BioX-Shanghai Team, the PDZ domain is located in Membrane Anchor 4, while the PDZ ligands on Membrane Anchor 3. Due to the fact that the PDZ domain and ligand is in the N-terminus of the whole protein, we added a SRP-dependent signal peptide, ssdsbA, to help the Membrane Anchor localize to the membrane correctly.

Related Parts

Part:BBa_K771104, PDZ ligand

Part:BBa_K771003, Membrane Anchor 3, containing PDZ ligand

Part:BBa_K771004, Membrane Anchor 4, containing PDZ domaim

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 115
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 345


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Categories
Parameters
n/assDsbA-PDZdomain