
Part:BBa_K916002
This part is a work in progress and does not yet work as expected.
See a full description of the progress on our wiki. |
TraR-CGFP Translational Fusion
NOTE: This part has no promoter, and so can be used with any BioBrick promoter in the registry. See the list of promoters here.

http://www.ncbi.nlm.nih.gov/pubmed/18481985
Usage and Biology
This is a fusion protein consisting of TraR from Agrobacterium tumefaciens and the C-terminal fragment of GFP (CGFP). This is for use with part BBa_K916001 as a GFP-fragment complementation based biosensor.
TraR is the A. tumefaciens transcriptional regulator responsible for its quorum sensing response. It binds N-(3-Oxo-octanoyl)-l-homoserine lactone (3-oxo-C8-HSL), and upon binding forms a homodimer. Most similar LuxR family quorum sensing regulators dimerize without their cognate autoinducer, but TraR will only dimerize upon binding 3-oxo-C8-HSL. We exploit this property of TraR by fusing it to two seperate fragments of GFP (NGFP and CGFP). Our expectation is that in the presence of its autoinducer, TraR will dimerize and bring the two fragments of GFP into proximity, allowing them to interact and fluoresce like an intact GFP peptide.
Please see the design notes and our wiki for additional information.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 984
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 910
None |