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Primers

Primers are not full BioBrick parts. They are short DNA sequences used for PCR. Primers are always "Available" since individual labs are expected to purchase them directly from an oligonucleotide supplier. Primers are not included in any Registry DNA distributions.

VF2 and VR (G00100 and G00101) are the most important primers for BioBrick work. They are used together to verify the length of a part in its plasmid. See the Physical DNA part page for the expected VF2-VR length of the part. VF2 and VR should also be used to sequence a part since they allow coverage of the BioBrick cloning site as well as the insert. They are positioned to allow for the typically poor sequencing results near the primer itself.

Available PrimersShow 1297 more partsEdit
NameDescriptionTargetF/R%GCTmLength
F-R
Length to Part
Start     End
Length

Edit Table specification