
Regulatory
Part:BBa_K1351018
Designed by: Philipp Popp & Roman Herzog Group: iGEM14_LMU-Munich (2014-09-15)
This part was generated with the RFC10 standard and has the following prefix and suffix:
prefix with EcoRI, NotI and XbaI: | GAATTCGCGGCCGCTTCTAGAG |
suffix with SpeI, NotI and PstI: | TACTAGTAGCGGCCGCTGCAG |
Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red.
This part is used in the 2014 LMU-Munich iGEM project BaKillus.
The Promoter regulates the sdpI (immunity against the cannibalism toxin sdpC) and the regulator sdpR in Bacillus subtilis. The overhangs were designed according to RFC10.
Primer fwd.: GAATTCGCGGCCGCTTCTAGAG gggttgtttttaaaaaaattcaagttataatg
Primer rev.: CTGCAGCGGCCGCTACTAGTA aaaaacatttagacatttagatatttgtataaatg
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/bsubtilis
//regulation
//regulation
None |