Coding

Part:BBa_K3981005

Designed by: Yulin Peng   Group: iGEM21_AHUT_China   (2021-08-27)


Vgb

Vireoscilla hemoglobin (VHb) of Gram-negative aerobic bacterium vireoscilla is the first bacterial hemoglobin found. Its function is to isolate oxygen, especially when it is at low concentration, and transport it to terminal respiratory oxidase, so as to enhance oxidative phosphorylation. Vitreoscilla hemoglobin gene (vgb) was introduced into E. coli, cell growth and target protein production increased significantly. The vgb promoter is regulated by oxygen at the transcriptional level, and hypoventilation is sufficient to induce VHb gene expression.[2] We used vgb promoter to replace the T7 promoter of pET-28A (+) to enable E. coli Nissle 1917 (ECN) to transcribe under hypoxic conditions.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None