
Coding
Part:BBa_K3134007
Designed by: Shiyuan Li Group: iGEM19_Nanjing_High_School (2019-10-16)
YF1
YF1 is especially sensitive to blue light. With FixJ, it can record the light. In the absence of blue light, it phosphorylates its cognate response regulator FixJ, which then drives robust gene expression from the FixK2 promoter. In the presence of blue light, however, net kinase activity of YF1 and consequent gene expression are greatly reduced.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 544
Illegal NgoMIV site found at 616
Illegal NgoMIV site found at 706
Illegal NgoMIV site found at 724
Illegal AgeI site found at 258 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 157
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Categories
Parameters
None |