diff --git a/wiki/pages/description.html b/wiki/pages/description.html index d69d625de419c6eb14ab76a9e73b09a8aeaf0a03..b1b4e981f133017a559a14bdfd3753aba1558052 100644 --- a/wiki/pages/description.html +++ b/wiki/pages/description.html @@ -58,7 +58,7 @@ <div class="col-lg-12"> <h2>System</h2> <hr> - <p>We aim to design plasmids with inserts of homologous variants on these three genes from species such as <i>B. thuringiensis</i> <a href="https://doi.org/10.1016/j.enzmictec.2022.109999"><sup>[11]</sup></a> and <i>T. pubescens</i> <a href="https://www.cabdirect.org/cabdirect/abstract/20183369455"><sup>[13]</sup></a> . The plasmid will first be transformed into DH5a E. coli for cloning, then electroporation for homologous recombination-based genomic insertion will be performed based on a protocol by Omayra et al. (2022) into the genome of S. elongatus. Expression of our vectors will be confirmed by PCR and MB-degrading activity will be quantified by spectrophotometric analysis. </p> + <p>We aim to design plasmids with inserts of homologous variants on these three genes from species such as <i>B. thuringiensis</i> <a href="https://doi.org/10.1016/j.enzmictec.2022.109999"><sup>[11]</sup></a> and <i>T. pubescens</i> <a href="https://www.cabdirect.org/cabdirect/abstract/20183369455"><sup>[14]</sup></a> . The plasmid will first be transformed into DH5a E. coli for cloning, then electroporation for homologous recombination-based genomic insertion will be performed based on a protocol by Omayra et al. (2022) into the genome of S. elongatus. Expression of our vectors will be confirmed by PCR and MB-degrading activity will be quantified by spectrophotometric analysis. </p> <p>MB degraded metabolites' toxicity has been found to be lower than that of the parent compound <a href="https://doi.org/10.1016/j.enzmictec.2022.109999"><sup>[11]</sup></a>. To investigate the toxicity of the degradation products, which have been proposed to be aromatic amines, raw and treated EC50 values will be used for comparison</p> </div> @@ -79,7 +79,7 @@ <ul>[10] Salimi, A., & Roosta, A. (2019). Experimental solubility and thermodynamic aspects of methylene blue in different solvents. Thermochimica Acta, 675, 134-139. https://doi.org/10.1016/j.tca.2019.03.024</ul> <ul>[11] Wu, K., Shi, M., Pan, X., Zhang, J., Zhang, X., Shen, T., & Tian, Y. (2022). Decolourization and biodegradation of methylene blue dye by a ligninolytic enzyme-producing Bacillus thuringiensis: degradation products and pathway. Enzyme and Microbial Technology, 156, 109999. https://doi.org/10.1016/j.enzmictec.2022.109999</ul> <ul>[12] Xu W. (2022). Current Situation and Prospect of waste-water Treatment in Dye Industry [J]. Dye Industry, 39(6): 35- 39.</ul> - <ul>[13] Zhang, T., Zhang, B., & Li, Y. (2018). Decolorization of thiazine dye methylene blue by three white-rot fungi. Mycosystema, 37(9), 1243-1250 https://www.cabdirect.org/cabdirect/abstract/20183369455</ul> + <ul>[14] Zhang, T., Zhang, B., & Li, Y. (2018). Decolorization of thiazine dye methylene blue by three white-rot fungi. Mycosystema, 37(9), 1243-1250 https://www.cabdirect.org/cabdirect/abstract/20183369455</ul> </div>