{% extends "layout.html" %} {% block title %}Special Prizes{% endblock %} {% block page_content %}
Within our parts collection, we curated a comprehensive list of alternative components including Reverse Transcriptase (RT), prime editing guide RNAs (pegRNAs), and nicking guide RNAs (ngRNAs).
The primary focus in this project has been on developing the alternative RTs, a crucial element of the Prime Editor (PE). This curated list encompasses a comprehensive range of Retroviral RTs sourced from various genera and host organisms, each characterized by distinct structures and lengths.
pegRNAs and ngRNAs play distinct roles, targeting specific endogenous sites and facilitating diverse types of edits. Notably, four of the provided RNA sequences were exclusively designed in our laboratory. They play a significant role in evaluating the performance of the Prime Editor under various conditions. Moreover, they enable us to pinpoint distinctive traits, such as proficiency in handling complex edits or displaying specific site preferences, etc.
Of particular significance is one of the designated sites (POAG), which holds therapeutic potential. This represents a significant stride towards advancing prime editing for practical therapeutic applications.
Registry Part ID | Name | Part Type |
---|---|---|
BBa_K4830000 | RNF pegRNA | pegRNA |
BBa_K4830001 | RNF pegRNA | ngRNA |
BBa_K4830002 | RNF2 AtoC pegRNA | pegRNA |
BBa_K4830003 | RNF2 AtoC ngRNA | ngRNA |
BBa_K4830004 | EMX1 pegRNA | pegRNA |
BBa_K4830005 | EMX1 ngRNA | ngRNA |
BBa_K4830006 | HEK3 pegRNA | pegRNA |
BBa_K4830007 | HEK3 ngRNA | ngRNA |
BBa_K4830008 | POAG snp1-3 pegRNA | pegRNA |
BBa_K4830009 | POAG snp1-3 ngRNA | ngRNA |
BBa_K4830010 | ADAR pegRNA | pegRNA |
BBa_K4830011 | ADAR ngRNA | ngRNA |
BBa_K4830012 | FANCF pegRNA | pegRNA |
BBa_K4830013 | FANCF ngRNA | ngRNA |
BBa_K4830014 | nickase Cas9 | Cas Nuclease |
BBa_K4830015 | PFV RT | Reverse transcriptase |
BBa_K4830016 | CMV enhancer and promoter | Promoter |
BBa_K4830017 | Ll.ltrA RT | Reverse Transcriptase |
BBa_K4830018 | MMTV RT | Reverse Transcriptase |
BBa_K4830019 | TLR pegRNA 1 | pegRNA |
BBa_K4830020 | TLR ngRNA 1 | ngRNA |
BBa_K4830021 | TLR pegRNA 2 | pegRNA |
BBa_K4830022 | TLR ngRNA 2 | ngRNA |
BBa_K4830023 | PETLR pegRNA 1 | pegRNA |
BBa_K4830024 | PETLR ngRNA 1 | ngRNA |
BBa_K4830025 | TLR-76 pegRNA | pegRNA |
BBa_K4830026 | 777BFP ngRNA | ngRNA |
BBa_K4830027 | mCherry_C-terminus stop codon_Clover | CDS |
BBa_K4830028 | eGFP_simple premature stop codon | CDS |
BBa_K4830029 | eGFP_complex premature stop codon | CDS |
BBa_K4830030 | MiniRT2 | Reverse transcriptase |
BBa_K4830031 | BLV RT | Reverse transcriptase |
BBa_K4830032 | CaMV RT | Reverse transcriptase |
BBa_K4830033 | RSV RT | Reverse transcriptase |
BBa_K4830034 | Line 1 RT | Reverse transcriptase |