{% extends "layout.html" %} {% block title %}Special Prizes{% endblock %} {% block page_content %}

Best Part Collection

Within our parts collection, we curated a comprehensive list of alternative components including Reverse Transcriptase (RT), prime editing guide RNAs (pegRNAs), and nicking guide RNAs (ngRNAs).

The primary focus in this project has been on developing the alternative RTs, a crucial element of the Prime Editor (PE). This curated list encompasses a comprehensive range of Retroviral RTs sourced from various genera and host organisms, each characterized by distinct structures and lengths.

pegRNAs and ngRNAs play distinct roles, targeting specific endogenous sites and facilitating diverse types of edits. Notably, four of the provided RNA sequences were exclusively designed in our laboratory. They play a significant role in evaluating the performance of the Prime Editor under various conditions. Moreover, they enable us to pinpoint distinctive traits, such as proficiency in handling complex edits or displaying specific site preferences, etc.

Of particular significance is one of the designated sites (POAG), which holds therapeutic potential. This represents a significant stride towards advancing prime editing for practical therapeutic applications.

Registry Part ID Name Part Type
BBa_K4830000 RNF pegRNA pegRNA
BBa_K4830001 RNF pegRNA ngRNA
BBa_K4830002 RNF2 AtoC pegRNA pegRNA
BBa_K4830003 RNF2 AtoC ngRNA ngRNA
BBa_K4830004 EMX1 pegRNA pegRNA
BBa_K4830005 EMX1 ngRNA ngRNA
BBa_K4830006 HEK3 pegRNA pegRNA
BBa_K4830007 HEK3 ngRNA ngRNA
BBa_K4830008 POAG snp1-3 pegRNA pegRNA
BBa_K4830009 POAG snp1-3 ngRNA ngRNA
BBa_K4830010 ADAR pegRNA pegRNA
BBa_K4830011 ADAR ngRNA ngRNA
BBa_K4830012 FANCF pegRNA pegRNA
BBa_K4830013 FANCF ngRNA ngRNA
BBa_K4830014 nickase Cas9 Cas Nuclease
BBa_K4830015 PFV RT Reverse transcriptase
BBa_K4830016 CMV enhancer and promoter Promoter
BBa_K4830017 Ll.ltrA RT Reverse Transcriptase
BBa_K4830018 MMTV RT Reverse Transcriptase
BBa_K4830019 TLR pegRNA 1 pegRNA
BBa_K4830020 TLR ngRNA 1 ngRNA
BBa_K4830021 TLR pegRNA 2 pegRNA
BBa_K4830022 TLR ngRNA 2 ngRNA
BBa_K4830023 PETLR pegRNA 1 pegRNA
BBa_K4830024 PETLR ngRNA 1 ngRNA
BBa_K4830025 TLR-76 pegRNA pegRNA
BBa_K4830026 777BFP ngRNA ngRNA
BBa_K4830027 mCherry_C-terminus stop codon_Clover CDS
BBa_K4830028 eGFP_simple premature stop codon CDS
BBa_K4830029 eGFP_complex premature stop codon CDS
BBa_K4830030 MiniRT2 Reverse transcriptase
BBa_K4830031 BLV RT Reverse transcriptase
BBa_K4830032 CaMV RT Reverse transcriptase
BBa_K4830033 RSV RT Reverse transcriptase
BBa_K4830034 Line 1 RT Reverse transcriptase
{% endblock %} {% block footerpage %} {% endblock %}