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End-over-end microcentrifuge tube spinner

Many protocols—in particular, protein purification—require constant, gentle mixing of small quantities of liquids for an extended period of time. We designed and produced an end-over-end microcentrifuge tube rotating device to enable our team to carry out protein purification. In our case, our goal was to elute purified IL-6 protein from lysate of HEK293 cells expressing an IL-6 gene, but our hardware can certainly be used for other protein purification and similar protocols!

Below is a video demonstrating how our rotator functions in practice.



With the understanding that many other teams may similarly lack access to industrial rotating devicecs, we have published all of the code, files, and documentation necessary for any future team to construct their own hardware. This includes design files for the 3D printing process, code for the electrical components, and clearly written explanation of how to piece together the various components of building this device. You can access all of this documentation at this repository.

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