{% extends "layout.html" %} {% block title %}Results{% endblock %} {% block lead %}You can describe the results of your project and your future plans here.{% endblock %} {% block page_content %}
Initial functioning and proof of concept experiments
Gel #1
Visible bands only in the DNA ladder lane. Our miRNA and experimental results are not visible.
Gel #2
Well #1 contained our Cas13+crRNA+miRNA reaction; well #2 contained just miRNA. The reaction mixture showed a bit of smearing, indicating possibly positive results. However, we cannot confirm that the smearing is due to successful cutting. We decided to run it again, this time with several other controls to help visualize and interpret the results. We also decided to add a denaturing step to prevent the miRNA from forming secondary structures and not separating properly in the gel.
Gel #3
8 of the 10 wells contained a product, with the first well being an RNA ladder. The ladder showed unsuccessful separating, indicating an issue with our gel or with excess ladder used. We also noticed the EtBr began running off the gel, cutting off visibility where we would see our results. We ran it again to correct the EtBr and ladder issues.
Gel #4
Ladder separated successfully. No product visible in any other well. This gel also contained random splotches of illumination. We did not denature the product for this gel, so we ran the experiment one last time to include that step.
Gel #5
Only visible bands in the ladder lane. We suspect that the gel ran for too long, causing our small product to run off the gel. If we had the time, we would run this again for a shorter amount of time.
Due to the timing of our project, we were unable to complete any more experiments. However, we have several directions we want to go with our project.