diff --git a/wiki/pages/composite.html b/wiki/pages/composite.html index 3ad8db48edf84f2f384e19cbce99b2a8b52960ae..4f610656d645eede857d919528627d425844429e 100644 --- a/wiki/pages/composite.html +++ b/wiki/pages/composite.html @@ -260,16 +260,15 @@ We hope this part will allow future iGEM teams to work with their local plant sp <figure> <img class="img-inline" - src="https://static.igem.wiki/teams/4729/wiki/composite-part/at-3and10days-psrk.png" + src="https://static.igem.wiki/teams/4729/wiki/composite-part/trafo3days.png" alt="" /> - <figcaption>Figure 7: Constructs containing combinations of the endogenous <i>A. rhizogenes</i> ARqua1 <i>virG</i>, <i>virG</i> (pTiBo542), <i>virG</i> (pTiBo542 N80D), and the promoters Ptau and Ptac. This combinatory library was then transformed in <i>A. rhizogenes</i> ARqua1 for determining if an increase of plant transformation efficiency could be detected.</figcaption> + <figcaption>Figure 7: Transformation efficiency after 3 days. Constructs containing combinations of the endogenous <i>A. rhizogenes</i> ARqua1 <i>virG</i>, <i>virG</i> (pTiBo542), <i>virG</i> (pTiBo542 N80D), and the promoters Ptau and Ptac. This combinatory library was then transformed in <i>A. rhizogenes</i> ARqua1 for determining if an increase of plant transformation efficiency could be detected.</figcaption> </figure> - <p> - After the initial observation of results three days post-transformation with Ptau_super80_pSRK (Figure 7A), Ptac_TiBo542_pSRK (Figure 7B) and Ptac_super80_pSRK (Figure 7C), there was a noticeable decline in transformation - efficiency which went <a class="igem" href="https://2023.igem.wiki/marburg/results">from previous 46% to a range between 29% to 37%</a> (Figure 7A-C). To address + After the initial observation of results three days post-transformation with Ptau_super80_pSRK , Ptac_TiBo542_pSRK and Ptac_super80_pSRK (Figure 7), there was a noticeable decline in transformation + efficiency which went <a class="igem" href="https://2023.igem.wiki/marburg/results">from previous 46% to a range between 29% to 37%</a> (Figure 7). To address this issue promptly and avoid any unnecessary delays, our team initiated troubleshooting procedures. This involved conducting stability assay tests to gain a deeper understanding of the factors contributing to the reduction in transformation efficiency. @@ -328,11 +327,18 @@ We hope this part will allow future iGEM teams to work with their local plant sp and plasmid loss seems to occur after 4 days. Moreover, the pABCa plasmid exhibited stability and demonstrated comparable transformation efficiency after 3 days when compared to constructs with the pSRK backbone. </p> - +<figure> + <img + class="img-inline" + src="https://static.igem.wiki/teams/4729/wiki/composite-part/trafo10days.png" + alt="" + /> + <figcaption>Figure 8: Trandformation efficiency after 10 days. Constructs containing combinations of the endogenous <i>A. rhizogenes</i> ARqua1 <i>virG</i>, <i>virG</i> (pTiBo542), <i>virG</i> (pTiBo542 N80D), and the promoters Ptau and Ptac. This combinatory library was then transformed in <i>A. rhizogenes</i> ARqua1 for determining if an increase of plant transformation efficiency could be detected.</figcaption> +</figure> <p> - Upon evaluating our most recent results 10 days post-transformation with constructs containing the pSRK backbone, we + Upon evaluating our most recent results 10 days post-transformation (see Figure 8) with constructs containing the pSRK backbone, we were surprised to witness an unexpected increase in the number of RUBY-positive plants compared to the 3-day - post-transformation results. <strong>We were especially surprised by the Ptac_TiBo542_pSRK transformation results which showed a + post-transformation results (see Figure7). <strong>We were especially surprised by the Ptac_TiBo542_pSRK transformation results which showed a transformation efficiency gain of 9% in comparison to the 35S:RUBY:KanR baseline</strong>. The current efficiency levels now seem to be on par with the outcomes from the baseline experiments. With the assumption that pABCa behaves similarly, we anticipate obtaining comparable results ten days after transformation, and we anticipate these results to be available