diff --git a/src/pages/measurement.mdx b/src/pages/measurement.mdx index 5f624b80646523a955ad1d650ae07a4fff7d25f9..ef87869e44198422dcaf59474dbf27619c5b91f5 100644 --- a/src/pages/measurement.mdx +++ b/src/pages/measurement.mdx @@ -58,7 +58,7 @@ All the codes, steps for assembly, and CAD files can be found in the open-source Last year, Micro-Q used a singular 405 nm laser for high-precision green fluorescent sample excitation, minimizing needed software processing. However, when presented to Kiatichai Faksri of Khon Kaen University in Thailand, he commended its single-tube quantification accuracy but noted difficulties switching excitation lasers and filters to quantify different materials. Commercial spectrophotometers utilize white light lasers, which cover the full visible spectrum, in conjunction with diffraction gratings to choose the optimal excitation wavelength for a given biological sample (Dondelinger, 2011). However, these lasers often exceed $3,000, as they combine an array of laser wavelengths into a single compressed laser. Additionally, the many moving parts make these systems prone to breaking, while increasing size and bulkiness. A cost-effective alternative is a 5000K LED emitting the full 350-750 nm visible spectrum (Liu et al., 2018). To test our LED, we excited green fluorescein dyes at 30°, 45°, and 60°, finding 45° optimal for fluorescence visualization with minimal glare (Fig. 3). Additionally, we coated the inside of Micro-Q Pro with Musou Black Paint, which absorbs 99% of visible light, to eliminate excess ambient light for clearer imaging (Chu, 2019). - + <Image src="https://static.igem.wiki/teams/4683/wiki/measurement/fig3-2.png"