diff --git a/src/pages/description.mdx b/src/pages/description.mdx
index 12f110d807ad639405edee1c76b01c19aa4f8709..76b193d08d22c2a36c42a7771e16fcc0785c7054 100644
--- a/src/pages/description.mdx
+++ b/src/pages/description.mdx
@@ -36,11 +36,11 @@ Currently, various detection and diagnosis methods are used for CAD. These inclu
 Coronary artery disease is caused by plaque buildup which is initially clustered in fat and blood cells in the body (see Fig. 2). As the collection worsens, arteries narrow in a process called atherosclerosis. The following describes the key process in three crucial steps from the normal condition of the artery (Shahjehan, 2023).
 
 <div className="pl-2">
- Formation - The first stages of the formation of atherosclerosis include the deposition of foam cells, also known as lipid macrophages (CDC, 2021). They disrupt the tunica intima, which is the innermost layer of the artery. Due to this disruption, a “fatty streak” is formed. 
+ Formation - The first stages of the formation of atherosclerosis include the deposition of foam cells, also known as lipid macrophages. They disrupt the tunica intima, which is the innermost layer of the artery. Due to this disruption, a “fatty streak” is formed (CDC, 2021).
 
- Progression - The progression of low-density lipoprotein (LDL) particles and foam cells activate T cells, a type of white blood cell that leads to immune responses promoting the nourishment of the plaque (CDC, 2021). Thus, the plaque’s size increases and this process is known as angiogenesis (CDC, 2021). 
+ Progression - The progression of low-density lipoprotein (LDL) particles and foam cells activate T cells, a type of white blood cell that leads to immune responses promoting the nourishment of the plaque. Thus, the plaque’s size increases and this process is known as angiogenesis (CDC, 2021). 
 
- Blockage - As the plaque continues to grow, it undergoes calcification which leads to the narrowing of the coronary arteries and restriction of blood flow (CDC, 2021). If there is complete blockage in the arteries, there is a severe chance of myocardial infarction, or a heart attack (CDC, 2021).
+ Blockage - As the plaque continues to grow, it undergoes calcification which leads to the narrowing of the coronary arteries and restriction of blood flow. If there is complete blockage in the arteries, there is a severe chance of myocardial infarction, or a heart attack (CDC, 2021).
 </div>
 
 <Image src='https://static.igem.wiki/teams/4683/wiki/homepage/pathophys.png' caption='Figure 2. The progression of CAD' />
diff --git a/src/pages/human-practices.mdx b/src/pages/human-practices.mdx
index fb207f5c68d5a79d50f4eba6d392da5a151997e3..ac520236614e6b86d9ae22bbd1c892c66e1440bf 100644
--- a/src/pages/human-practices.mdx
+++ b/src/pages/human-practices.mdx
@@ -18,7 +18,9 @@ import Reference from "../components/md_components/Reference";
 
 Last year, Lambert iGEM identified patients as the end goal of CADlock’s implementation. In order to accomplish this, we interviewed multiple leading microRNA researchers and cardiologists, who contributed to identifying the direction of our project as a point-of-care diagnostic screening tool to track the progression of coronary artery disease (CAD). Inspired to expand last year’s project with a focus on inclusivity, we improved and implemented wet lab mechanisms, including exponential rolling circle amplification (eRCA) from RCA [see RCA](https://2023.igem.wiki/lambert-ga/rca/). We also used protein purification to create our own phi29 DNA polymerase to minimize reaction costs. In addition, we expanded upon MicroQ, implementing an adaptation called MicroQ Pro. Furthermore, after talking with a graduate student, we developed LabPilot, an automated pipetter. We created a podcast and spoke to multiple doctors and professionals about the direction of our project. Drawing on the expertise of exceptional stakeholders, our journey transformed from conceptualization to implementation (see Fig. 1). 
 
-<Image src='https://static.igem.wiki/teams/4683/wiki/human-practices/hp-graphic-1.png' caption='Figure 1. Overview graphic of the 2023 inclusivity approach.' />
+<Image src='https://static.igem.wiki/teams/4683/wiki/human-practices/hp-graphic-1.png' 
+size="800"
+caption='Figure 1. Overview graphic of the 2023 inclusivity approach.' />
 
 
 ## Doctors
@@ -27,7 +29,8 @@ Last year, Lambert iGEM identified patients as the end goal of CADlock’s imple
 
 We spoke to Dr. Mindy B. Gentry, a cardiologist in Marietta, Georgia,  about sex inclusivity and CAD. We originally planned to use a gene called SVEP1, a gene that influences atherosclerosis. Specifically, SVEP1 expresses a protein that stimulates the development of plaque in arteries. This gene has a negative correlation with a miRNA named miR-1269b.  Instead, she guided us to look at CAD correlation with an emphasis on estrogen-induced pathways. With her help, we were able to find a correlation between CAD and an estrogen-specific miRNA. Dr. Gentry, who runs a female cardiac health center, expressed the need for proactive measures to be taken to preserve heart health for females. The incorporation of Lambert iGEM’s estrogen initiative, guided by her aid, strengthens the proactive approach by making a diagnostic for a specialized risk group.
 
-<Image src='https://static.igem.wiki/teams/4683/wiki/human-practices/gentry.png' caption='Figure 2. Dr. Mindy B. Gentry' />
+<Image src='https://static.igem.wiki/teams/4683/wiki/human-practices/gentry.png' 
+caption='Figure 2. Dr. Mindy B. Gentry' />
 
 ### Dr. Fairweather
 
diff --git a/src/pages/inclusivity.mdx b/src/pages/inclusivity.mdx
index 94e9b13e97c1538854eb826cbfa2b65924c0f2df..efbeeb3efef122143912ba4a9faaa72718cfadf9 100644
--- a/src/pages/inclusivity.mdx
+++ b/src/pages/inclusivity.mdx
@@ -16,6 +16,7 @@ Healthcare is broken down into one of two categories: reactive and proactive. Th
 
 <Image
   src="https://static.igem.wiki/teams/4683/wiki/inclusivity/hp-hierarchy.png"
+size="600"
   caption="Figure 1. The expansion of our inclusivity focus this year, combining accessibility, affordability, and accuracy under proactivity."
 />
 
@@ -44,11 +45,11 @@ Preventative care is two-fold, relying on early detection and educating patients
 
 ## RCA
 
-Furthering last year's approach with accessibility, affordability, and accuracy, we continued utilizing and improving RCA to assess CAD risk groups while also addressing underrepresented demographics of people. Improvements to last year’s RCA mechanism include testing the biosensor’s specificity, comparing reporter mechanisms, and optimizing reaction times. In addition to RCA modifications, Lambert iGEM pursued a new wet-lab initiative called exponential rolling circle amplification (eRCA). eRCA provides a more sensitive reading of microRNAs with a faster reading time. The emphasis on additional accuracy through eRCA and affordability to end users enables CADlock to be accessible to a wide range of demographics, not limited to race, sex, and economic status (See Wetlab: eRCA).
+Furthering last year's approach with accessibility, affordability, and accuracy, we continued utilizing and improving RCA to assess CAD risk groups while also addressing underrepresented demographics of people. Improvements to last year’s RCA mechanism include testing the biosensor’s specificity, comparing reporter mechanisms, and optimizing reaction times. In addition to RCA modifications, Lambert iGEM pursued a new wet-lab initiative called exponential rolling circle amplification (eRCA). eRCA provides a more sensitive reading of microRNAs with a faster reading time. The emphasis on additional accuracy through eRCA and affordability to end users enables CADlock to be accessible to a wide range of demographics, not limited to race, sex, and economic status (See [RCA: eRCA](https://2023.igem.wiki/lambert-ga/rca/)).
 
 ## Capillary
 
-Lambert iGEM explored optimizing the reaction time and resources by running the reaction in capillary tubes instead of PCR tubes for DNA amplification and imaging. This quicker response time allows for more patients to be tested in a point-of-care setting. This increase in healthcare accessibility and thus patient retention time allows for an emphasis to be placed on proactive care. MicroRNAs (miRNAs) can easily diffuse through the capillary tube due to the tube's small diameter. This results in high amounts of DNA in isolated locations (see Fig. 4). When exposed to a specific wavelength of light, individual specks of DNA can be seen by the naked eye. DNA can then be counted without the use of complex fluorescence quantification devices such as fluorometers or plate readers, which makes the test more accessible and lowers the technology barrier. Overall, capillary amplification is a reliable, efficient, and user-friendly assay. (See Wetlab: Capillary)
+Lambert iGEM explored optimizing the reaction time and resources by running the reaction in capillary tubes instead of PCR tubes for DNA amplification and imaging. This quicker response time allows for more patients to be tested in a point-of-care setting. This increase in healthcare accessibility and thus patient retention time allows for an emphasis to be placed on proactive care. MicroRNAs (miRNAs) can easily diffuse through the capillary tube due to the tube's small diameter. This results in high amounts of DNA in isolated locations (see Fig. 4). When exposed to a specific wavelength of light, individual specks of DNA can be seen by the naked eye. DNA can then be counted without the use of complex fluorescence quantification devices such as fluorometers or plate readers, which makes the test more accessible and lowers the technology barrier. Overall, capillary amplification is a reliable, efficient, and user-friendly assay. [See Wetlab: Capillary](https://2023.igem.wiki/lambert-ga/rca/).
 
 <Image
   src="https://static.igem.wiki/teams/4683/wiki/inclusivity/screenshot-2023-10-11-102911.png"
@@ -58,11 +59,11 @@ size="500"
 
 ## Protein Purification
 
-In addition to frugal technologies, Lambert iGEM aimed to decrease the cost of Rolling Circle Amplification (RCA) reagents by producing the enzymes required through protein purification. Phi29 DNA polymerase, priced at $251.00 for 1,250 units from New England Biolabs, not only covers the majority of the cost of RCA reagents but also possesses invaluable qualities such as strand displacement and processive synthesis properties that are necessary for RCA (New England Biolabs, n.d). By producing phi29 DNA polymerase through protein purification, combined with the accuracy of exponential RCA, Lambert iGEM aims to  increase the affordability and in turn accessibility, both of which are contributing factors to inclusive care. This allows for more tests because the proteins are much more affordable, increasing the preventative aspect of healthcare. (See Wetlab: Protein Purification)
+In addition to frugal technologies, Lambert iGEM aimed to decrease the cost of Rolling Circle Amplification (RCA) reagents by producing the enzymes required through protein purification. Phi29 DNA polymerase, priced at $251.00 for 1,250 units from New England Biolabs, not only covers the majority of the cost of RCA reagents but also possesses invaluable qualities such as strand displacement and processive synthesis properties that are necessary for RCA (New England Biolabs, n.d). By producing phi29 DNA polymerase through protein purification, combined with the accuracy of exponential RCA, Lambert iGEM aims to  increase the affordability and in turn accessibility, both of which are contributing factors to inclusive care. This allows for more tests because the proteins are much more affordable, increasing the preventative aspect of healthcare. [See Wetlab: Protein Purification](https://2023.igem.wiki/lambert-ga/protein-purification/).
 
 ## Estrogen
 
-While researching CAD risk factors and statistics, we came across literature that informed us about disparities among the sexes concerning early CAD diagnosis. We first conferred with Dr. Mindy Gentry, a women’s cardiologist, to get her opinion on the sex differences in cardiovascular health. She confirmed our insights about estrogen being a factor in increased susceptibility to CAD in women. To further our scientific research, we spoke to Dr. DeLisa Fairweather, a director of translational research in the Department of Cardiovascular Medicine at Mayo Clinic's Florida campus. She educated us on estrogen and its role as a CAD suppressor, and explained how women after menopause are more likely to develop heart disease because estrogen levels drastically decrease after menopause, causing women to be more likely to develop inflammation. Dr. Fairweather detailed that women who have inflammation in the heart are more likely to develop autoimmune disease and CAD. Additionally, she also guided us in the further development of our inclusivity by discussing how the hormone affects men as well. She told us that since men have a low estrogen level that also remains stable, they would be an ideal demographi to test for consistency in results, unlike women who have fluctuating levels of the hormone. Under the guidance of Dr. Fairweather, we tested miRNA-20b using Rolling Circle Amplification. In the presence of estrogen, CAD suppressors are activated and miRNA-20b is produced. miR-20b causes a negative feedback loop and suppresses estrogen, which will in turn inhibit the CAD suppressors. Dr. Fairweather explained how in men, a high level of miR-20b means that they are likely to develop CAD, however, in premenopausal women with inflammation symptoms, miR-20b protects them from developing CAD. She explained how if there is a high level of 20b, it will inhibit the estrogen from causing a catalyst between the developing autoimmune disease and CAD. We tested miR-20b with the RCA mechanism and ran a 1% gel to validate the presence of our product (see Fig. 5). Ultimately, increasing the factors that can be tested to signify CAD correlation encompasses a wider yet specified group allows for quality proactive care to be implemented. (See Wetlab: Inclusivity) 
+While researching CAD risk factors and statistics, we came across literature that informed us about disparities among the sexes concerning early CAD diagnosis. We first conferred with Dr. Mindy Gentry, a women’s cardiologist, to get her opinion on the sex differences in cardiovascular health. She confirmed our insights about estrogen being a factor in increased susceptibility to CAD in women. To further our scientific research, we spoke to Dr. DeLisa Fairweather, a director of translational research in the Department of Cardiovascular Medicine at Mayo Clinic's Florida campus. She educated us on estrogen and its role as a CAD suppressor, and explained how women after menopause are more likely to develop heart disease because estrogen levels drastically decrease after menopause, causing women to be more likely to develop inflammation. Dr. Fairweather detailed that women who have inflammation in the heart are more likely to develop autoimmune disease and CAD. Additionally, she also guided us in the further development of our inclusivity by discussing how the hormone affects men as well. She told us that since men have a low estrogen level that also remains stable, they would be an ideal demographic to test for consistency in results, unlike women who have fluctuating levels of the hormone. Under the guidance of Dr. Fairweather, we tested miRNA-20b using Rolling Circle Amplification. In the presence of estrogen, CAD suppressors are activated and miRNA-20b is produced. miR-20b causes a negative feedback loop and suppresses estrogen, which will in turn inhibit the CAD suppressors. Dr. Fairweather explained how in men, a high level of miR-20b means that they are likely to develop CAD, however, in premenopausal women with inflammation symptoms, miR-20b protects them from developing CAD. She explained how if there is a high level of 20b, it will inhibit the estrogen from causing a catalyst between the developing autoimmune disease and CAD. We tested miR-20b with the RCA mechanism and ran a 1% gel to validate the presence of our product (see Fig. 5). Ultimately, increasing the factors that can be tested to signify CAD correlation encompasses a wider yet specified group, allowing for quality proactive care to be implemented. [See Wetlab: Inclusivity](https://2023.igem.wiki/lambert-ga/rca/). 
 
 <Image
   src="https://static.igem.wiki/teams/4683/wiki/inclusivity/gel1.png"
@@ -73,7 +74,7 @@ While researching CAD risk factors and statistics, we came across literature tha
 
 ## CADmir
 
-Lambert iGEM developed CADmir as a centralized microRNA (miRNA) database to enhance researchers’ access to functional information about miRNAs more efficiently. (see Fig. 6) Currently, valuable insights on miRNAs are dispersed throughout academic literature, posing a challenge for researchers seeking detailed information about specific miRNAs. CADmir aims to address these issues by offering a cohesive and easily accessible platform that organizes all of the current scientific literature about miRNAs, facilitating quick and efficient searches. Lambert iGEM hopes that with CADmir, more researchers will explore miRNA research and incorporate miRNAs into more projects without requiring substantial amounts of time dedicated to reading through papers. CADmir aims to advance the field of miRNA research by improving information accessibility and reducing the time barrier associated with obtaining high-quality scientific information on these topics.(See Wetlab: Estrogen).
+Lambert iGEM developed CADmir as a centralized microRNA (miRNA) database to enhance researchers’ access to functional information about miRNAs more efficiently. (see Fig. 6) Currently, valuable insights on miRNAs are dispersed throughout academic literature, posing a challenge for researchers seeking detailed information about specific miRNAs. CADmir aims to address these issues by offering a cohesive and easily accessible platform that organizes all of the current scientific literature about miRNAs, facilitating quick and efficient searches. Lambert iGEM hopes that with CADmir, more researchers will explore miRNA research and incorporate miRNAs into more projects without requiring substantial amounts of time dedicated to reading through papers. CADmir aims to advance the field of miRNA research by improving information accessibility and reducing the time barrier associated with obtaining high-quality scientific information on these topics. [See Drylab: Software](https://2023.igem.wiki/lambert-ga/software/). 
 
 <Image
   src="https://static.igem.wiki/teams/4683/wiki/inclusivity/cadmir-time.png"
@@ -82,11 +83,11 @@ Lambert iGEM developed CADmir as a centralized microRNA (miRNA) database to enha
 
 ## LabPilot
 
-LabPilot was targeted towards underfunded laboratories and independent researchers to increase accessibility to this crucial equipment. The leading retailing liquid handler costs around \$10,000, prohibiting less-privileged laboratories from purchasing liquid handlers and instead manually pipetting (OT-2 Robot, 2023). LabPilot is primarily made with 3D printed parts, making it affordable and easily replicable by any community with a 3D printer at a build cost of \$250 and a pipette cost of \$409 (Eppendorf Research Plus Adjustable Volume, Single Channel Pipette, 2 - 20 uL, Yellow)(See Parts List), and about 6.5% the cost of a commercial device (OT-2 Robot). LabPilot upholds the standard of accuracy found in other liquid handlers by having a significant difference when comparing the volume of liquid pipetted by LabPilot and a human (See Proof of Concept). LabPilot’s intuitive software utilizes critical user experience (UX) principles: a user can quickly transfer liquid from one location to another with just three taps. Additionally, LabPilot’s software conforms to the Web Content Accessibility Guidelines 2.1 standards, ensuring a quality user experience for people with disabilities. (See Hardware: LabPilot)
+LabPilot was targeted towards underfunded laboratories and independent researchers to increase accessibility to this crucial equipment. The leading retailing liquid handler costs around \$10,000, prohibiting less-privileged laboratories from purchasing liquid handlers and instead manually pipetting (OT-2 Robot, 2023). LabPilot is primarily made with 3D printed parts, making it affordable and easily replicable by any community with a 3D printer at a build cost of \$250 and a pipette cost of \$409 (Eppendorf Research Plus Adjustable Volume, Single Channel Pipette, 2 - 20 uL, Yellow)(See Parts List), and about 6.5% the cost of a commercial device (OT-2 Robot). LabPilot upholds the standard of accuracy found in other liquid handlers by having a significant difference when comparing the volume of liquid pipetted by LabPilot and a human (See Proof of Concept). LabPilot’s intuitive software utilizes critical user experience (UX) principles: a user can quickly transfer liquid from one location to another with just three taps. Additionally, LabPilot’s software conforms to the Web Content Accessibility Guidelines 2.1 standards, ensuring a quality user experience for people with disabilities. (See Hardware: LabPilot) [See Drylab: Hardware](https://2023.igem.wiki/lambert-ga/hardware/). 
 
 ## Micro-Q
 
-Many medical diagnostics utilize fluorescence reporter mechanisms with mathematical models to quantify substances. However, commercial fluorometers are often bulky and cost over \$10,000, making these diagnostics inaccessible. Last year, Micro-Q exhibited single-tube green fluorescence quantification for \$16 but was limited to one sample. This year, Micro-Q Plus allows multi-sample, multi-spectral quantification with camera based fluorescence detection enabling automatic generation of fluorescence/protein expression curves. All these capabilities fit in a pocket-sized portable box, increasing accessibility to underfunded labs and the public. (See Measurement: MicroQ)
+Many medical diagnostics utilize fluorescence reporter mechanisms with mathematical models to quantify substances. However, commercial fluorometers are often bulky and cost over \$10,000, making these diagnostics inaccessible. Last year, Micro-Q exhibited single-tube green fluorescence quantification for \$16 but was limited to one sample. This year, Micro-Q Plus allows multi-sample, multi-spectral quantification with camera based fluorescence detection enabling automatic generation of fluorescence/protein expression curves. All these capabilities fit in a pocket-sized portable box, increasing accessibility to underfunded labs and the public. (See Measurement: MicroQ)[See Drylab: Measurement](https://2023.igem.wiki/lambert-ga/measurement/). 
 
 # Education
 
@@ -136,10 +137,11 @@ Human Practices involves speaking with various professionals, doctors, and resea
 
 ## Heart Health Awareness month
 
-Lambert iGEM planned to expand upon the SynbioOutLoud podcast by hosting an audience consisting of Lambert High School’s HOSA chapter, Science Olympiad, and Women in STEM club members to listen to highlighted guest speakers and professionals in the field. However, we wanted to expand the outreach by increasing the number of people who had access to this opportunity, so we converted the event to virtual webinars that focus on all aspects of heart health, both proactive and reactive, for the entire month of September. As the name suggests, the “Science for All” purpose was to access a wider audience, thus further creating an inclusive approach to education. Lambert iGEM hosted webinars discussing the importance of nutrition and exercise to prevent heart disease, and the process of First Aid/CPR/AED as a response system for when heart emergencies occur. Lambert iGEM was also accompanied by the American Heart Association (AHA), who co-hosted the webinar, teaching the audience about emergency response and CPR. While the members were not certified due to safety concerns, they were equipped with the proper knowledge to be an upstander rather than a bystander in the event of a cardiac emergency (see Fig. 11). To conclude the event, we specifically presented our gender research of all iGEM teams, along with the analysis of the impact created and the perceived trend for future demographic distribution in science roles. By advertising the “Science for All'' webinars through the county for greater demographic participation, Lambert iGEM was able to address a larger number of people through social media connections, the involvement of the AHA in promoting the webinar, and outreach towards other STEM clubs such as Women in STEM, Science Olympiad, and HOSA.  This event attempts to spread the knowledge of synthetic biology and STEM. We are also currently in the process of updating a formal inclusive Cardiac Emergency Response Plan to be implemented and piloted at Lambert High School.
+Lambert iGEM planned to expand upon the SynbioOutLoud podcast by hosting an audience consisting of Lambert High School’s HOSA chapter, Science Olympiad, and Women in STEM club members to listen to highlighted guest speakers and professionals in the field. However, we wanted to expand the outreach by increasing the number of people who had access to this opportunity, so we converted the event to virtual webinars that focus on all aspects of heart health, both proactive and reactive, for the entire month of September. As the name suggests, the “Science for All” purpose was to access a wider audience, thus further creating an inclusive approach to education. Lambert iGEM hosted webinars discussing the importance of nutrition and exercise to prevent heart disease, and the process of First Aid/CPR/AED as a response system for when heart emergencies occur. Lambert iGEM was also accompanied by the American Heart Association (AHA), who co-hosted the webinar, teaching the audience about emergency response and CPR. While the members were not certified due to safety concerns, they were equipped with the proper knowledge to be an upstander rather than a bystander in the event of a cardiac emergency (see Fig. 11). To conclude the event, we specifically presented our gender research of all iGEM teams, along with the analysis of the impact created and the perceived trend for future demographic distribution in science roles. By advertising the “Science for All'' webinars through the county for greater demographic participation, Lambert iGEM was able to address a larger number of people through social media connections, the involvement of the AHA in promoting the webinar, and outreach towards other STEM clubs such as Women in STEM, Science Olympiad, and HOSA. This event attempts to spread the knowledge of synthetic biology and STEM. We are also currently in the process of updating a formal inclusive Cardiac Emergency Response Plan to be implemented and piloted at Lambert High School.
 
 <Image
   src="https://static.igem.wiki/teams/4683/wiki/inclusivity/webinar-listenres.png"
+size="600"
   caption="Figure 11. Webinar participants at our school receive prizes for answering questions correctly. "
 />
 
diff --git a/src/pages/measurement.mdx b/src/pages/measurement.mdx
index 72c069e97f0edf4fef19c4a32be1788cb229635d..5867a0c7d13ed3c93e16d38eabbeb759c0dc34ec 100644
--- a/src/pages/measurement.mdx
+++ b/src/pages/measurement.mdx
@@ -1,7 +1,7 @@
 ---
 layout: ../layouts/PageLayout.astro
 title: Measurement
-header: https://static.igem.wiki/teams/4683/wiki/header-images/screen-shot-2023-10-11-at-9-18-19-pm.png
+header: https://static.igem.wiki/teams/4683/wiki/hardware/image-10-12-23-at-11-19-am.jpg
 ---
 
 import Wip from "../components/md_components/Wip";
@@ -17,8 +17,10 @@ Traditional methods of diagnosing coronary artery disease (CAD) such as angiogra
 
 In 2022, our team developed Micro-Q: a ~$15 PCR-tube fluorometer capable of quantifying fluorescent samples in seconds with 97.01% accuracy (See Lambert iGEM Hardware, 2022). This affordable solution, paired with its user-friendly mobile app, increases the accessibility of POCT in underfunded labs. However, after testing Micro-Q in Thailand, a couple of limitations were revealed: 1) quantifying batches of samples is tedious; 2) tuning excitation spectra for diverse fluorophores is complicated. Ultimately, these issues make testing and experimentation inefficient.
 
+
 This year, we addressed these issues by developing Micro-Q Pro—a camera-based fluorescence viewer under $10 capable of quantifying up to 8 PCR tubes simultaneously across the full visible wavelength spectrum. Since this detection uses camera imaging rather than photodiodes, users can record fluorescence videos and create real-time curves by snipping images at certain time points with 95.37% accuracy. This open-sourced fluorometer enables accurate and rapid sample quantification for diverse applications.
 
+
 ## Fluorescence Background
 
 Fluorescence is a type of luminescence that occurs when particular substances absorb light at one wavelength and then emit light at a longer wavelength. When a fluorescent sample is exposed to a specific excitation wavelength, it absorbs the light energy, raising its molecules to higher energy states. As these excited molecules return to lower energy ground states, they release excess energy as fluorescent light (see Fig. 1).
@@ -27,104 +29,91 @@ This fundamental process enables diverse applications for fluorescence, includin
 
 <Image
   src="https://static.igem.wiki/teams/4683/wiki/micro-q-figure-demonstrates-the-process-of-how-fluorescence-is-emitted-from-a-sample-2.png"
-
-caption="Figure 1. The process of fluorescence excitation and emission from a sample."
+size=""
+  caption="Figure 1. The process of fluorescence excitation and emission from a sample."
 />
 
+
+
 ## Design
 
-<ImageCarousel
-  client:load
-  caption="Figure 2. Different angles of Micro-Q Pro design"
-  images={[
-    "https://static.igem.wiki/teams/4683/wiki/measurement/fig2-1.jpg",
-    "https://static.igem.wiki/teams/4683/wiki/measurement/fig2-2.jpg",
-    "https://static.igem.wiki/teams/4683/wiki/measurement/fig2-3-1.jpg",
-    "https://static.igem.wiki/teams/4683/wiki/measurement/fig2-4.jpg",
-    "https://static.igem.wiki/teams/4683/wiki/measurement/fig2-5-1.jpg",
-    "https://static.igem.wiki/teams/4683/wiki/measurement/fig2-6.jpg",
-  ]}
-/>
+<ImageCarousel client:load caption="Figure 2. Different angles of Micro-Q Pro design" images={[ "https://static.igem.wiki/teams/4683/wiki/measurement/fig2-1.jpg", "https://static.igem.wiki/teams/4683/wiki/measurement/fig2-2.jpg", "https://static.igem.wiki/teams/4683/wiki/measurement/fig2-3-1.jpg", "https://static.igem.wiki/teams/4683/wiki/measurement/fig2-4.jpg", 
+"https://static.igem.wiki/teams/4683/wiki/measurement/fig2-5-1.jpg", 
+"https://static.igem.wiki/teams/4683/wiki/measurement/fig2-6.jpg"]}/>
 
 ### Parts List
+|Part|Price|
+|-----|-----|
+|3D-printed shell PLA (217.25g)|$2.33|
+|5000K LED Strip (4 inches)|$0.68|
+|Musou Black Paint (0.1 Fl Oz)| $0.58|
+|ESP-32 Camera |$6.33|
+|__Total:__ |__$9.92__|
+
+All the codes, steps for assembly, and CAD files can be found in the open-source Github repository:  [Micro-Q Pro Files](https://github.com/Lambert-iGEM-2023/Micro-Q-Pro). 
 
-| Part                           | Price     |
-| ------------------------------ | --------- |
-| 3D-printed shell PLA (217.25g) | $2.33     |
-| 5000K LED Strip (4 inches)     | $0.68     |
-| Musou Black Paint (0.1 Fl Oz)  | $0.58     |
-| ESP-32 Camera                  | $6.33     |
-| **Total:**                     | **$9.92** |
 
-All the codes, steps for assembly, and CAD files can be found in the open-source Github repository: [Micro-Q Pro Files](https://github.com/Lambert-iGEM-2023/Micro-Q-Pro).
 
 ### Light Source
 
 Last year, Micro-Q used a singular 405 nm laser for high-precision green fluorescent sample excitation, minimizing needed software processing. However, when presented to Kiatichai Faksri of Khon Kaen University in Thailand, he commended its single-tube quantification accuracy but noted difficulties switching excitation lasers and filters to quantify different materials.
 Commercial spectrophotometers utilize white light lasers, which cover the full visible spectrum, in conjunction with diffraction gratings to choose the optimal excitation wavelength for a given biological sample (Dondelinger, 2011). However, these lasers often exceed $3,000, as they combine an array of laser wavelengths into a single compressed laser. Additionally, the many moving parts make these systems prone to breaking, while increasing size and bulkiness. A cost-effective alternative is a 5000K LED emitting the full 350-750 nm visible spectrum (Liu et al., 2018). To test our LED, we excited green fluorescein dyes at 30°, 45°, and 60°, finding 45° optimal for fluorescence visualization with minimal glare (Fig. 3). Additionally, we coated the inside of Micro-Q Pro with Musou Black Paint, which absorbs 99% of visible light, to eliminate excess ambient light for clearer imaging (Chu, 2019).
+ 
 
 <Image
   src="https://static.igem.wiki/teams/4683/wiki/measurement/fig3-2.png"
-  caption="Figure 3. Fluorescein sample excited at A) 30° B) 45° C) 60°; 45° showed least glare and best fluorescence."
-  size="350"
+  caption="Figure 3. Fluorescein sample excited at A) 30° B) 45° C) 60°; 45° showed least glare and best fluorescence." 
+size: "350"
 />
 
 ### Fluorescence Quantification
-
 The original Micro-Q design utilized a photoresistor (a light intensity sensor) behind an emission filter to quantify the fluorescence intensity of the sample. While this design worked relatively well for discerning between high fluorescence concentrations, it struggled to differentiate between lower ones. Its accuracy could significantly be improved by replacing the photoresistor with a photodiode (a more sensitive light intensity sensor), however this year, Lambert iGEM implemented a camera-based detection system to enable real-time visualizations and quantification of sample fluorescence. Additionally, with this quantification mechanism, more samples can be quantified in a fraction of the time. This camera-based detection enables users to automate fluorescence curves by quantifying fluorescence across video segments.
 
-To quantify fluorescence, the Micro-Q Pro ESP32 camera captures two images: 1) a blank image with just light; 2) a sample image with fluorescent tubes (Fig. 4). Then, the algorithm subtracts the blank pixels (image 1) from the sample image (image 2) to remove non-fluorescent features, and isolate the sample's fluorescence intensity. The image is then preprocessed to improve quality before calculating RFU values. Preprocessing involves median blurring to reduce noise, inverting the image, and applying gamma correction. This compensates for the non-linear relationship between true and measured sample brightness (see Fig. 5).
+To quantify fluorescence, the Micro-Q Pro ESP32 camera captures two images: 1) a blank image with just light; 2) a sample image with fluorescent tubes (Fig. 4). Then, the algorithm subtracts the blank pixels (image 1) from the sample image (image 2) to remove non-fluorescent features, and isolate the sample's fluorescence intensity. The image is then preprocessed to improve quality before calculating RFU values. Preprocessing involves median blurring to reduce noise, inverting the image, and applying gamma correction. This compensates for the non-linear relationship between true and measured sample brightness (see Fig. 5). 
 
 <Image
   src="https://static.igem.wiki/teams/4683/wiki/measurement/fig4.png"
   caption="Figure 4. Software pipeline of Micro-Q Pro ESP-32 Camera."
 />
 
+
 <Image
   src="https://static.igem.wiki/teams/4683/wiki/measurement/fig5-1.png"
   caption="Figure 5. Tubes of fluorescein from 200µm 1.5µm a) before preprocessing b) after preprocessing."
 />
 
-After preprocessing, Otsu's thresholding masks out the remaining background noise, leaving only the foreground fluorescence signal. Individual binary masks are created for each PCR tube, and the average pixel brightness within each mask is calculated to determine the RFU values (Fig. 6). Since the masking algorithm will isolate the sample of fluorescence from excess light, users can quantify multiple samples without emission filters, unless a tube contains multiple fluorophores.
+
+After preprocessing, Otsu's thresholding masks out the remaining background noise, leaving only the foreground fluorescence signal. Individual binary masks are created for each PCR tube, and the average pixel brightness within each mask is calculated to determine the RFU values (Fig. 6). Since the masking algorithm will isolate the sample of fluorescence from excess light, users can quantify multiple samples without emission filters, unless a tube contains multiple fluorophores. 
 
 <Image
   src="https://static.igem.wiki/teams/4683/wiki/measurement/fig6-1.png"
   caption="Figure 6. Tubes of fluorescein from 200µm 1.5µm a) original b) after masking fluorescence."
 />
 
-### 3D-Printed Shell:
 
-The original Micro-Q device was initially designed to easily quantify individual PCR tubes. To enable multisample and multispectral quantification, Micro-Q Pro was redesigned as a 10.8 cm x 8.7cm x 8.3 cm 3D printed box with three key compartments. A sample chamber, an exchangeable emission filter, and a camera holster (see Fig. 7). The sample chamber was modified to hold a strip of 8 PCR tubes separated by dividers to minimize crosstalk between fluorescent signals, which could skew the data (Arrpe et al. 2017).
+### 3D-Printed Shell:
+The original Micro-Q device was initially designed to easily quantify individual PCR tubes. To enable multisample and multispectral quantification, Micro-Q Pro was redesigned as a 10.8 cm x  8.7cm x 8.3 cm 3D printed box with three key compartments. A sample chamber, an exchangeable emission filter, and a camera holster (see Fig. 7). The sample chamber was modified to hold a strip of 8 PCR tubes separated by dividers to minimize crosstalk between fluorescent signals, which could skew the data (Arrpe et al. 2017).
 
 Moreover, Micro-Q Pro uses a 45-degree angled white LED strip paired with the emission filter to detect fluorescence across all relevant wavelengths. The ESP-32 camera module is placed 7.8 cm away from the samples to ensure it can view all 8 tubes at once. These optimized elements work seamlessly together to enable robust, high-quality fluorescence imaging of multiple samples.
 
-<ImageCarousel
-  client:load
-  caption="Figure 7. Schematic diagram of the redesigned Micro-Q Pro device highlighting the sample chamber, emission filter compartment, and camera holster."
-  images={[
-    "https://static.igem.wiki/teams/4683/wiki/measurement/fig7-1.jpg",
-    "https://static.igem.wiki/teams/4683/wiki/measurement/fig7-2.jpg",
-    "https://static.igem.wiki/teams/4683/wiki/measurement/fig7-3.jpg",
-    "https://static.igem.wiki/teams/4683/wiki/measurement/fig7-4.jpg",
-  ]}
-/>
+<ImageCarousel client:load caption="Figure 7. Schematic diagram of the redesigned Micro-Q Pro device highlighting the sample chamber, emission filter compartment, and camera holster." images={[ "https://static.igem.wiki/teams/4683/wiki/measurement/fig7-1.jpg", "https://static.igem.wiki/teams/4683/wiki/measurement/fig7-2.jpg", "https://static.igem.wiki/teams/4683/wiki/measurement/fig7-3.jpg", "https://static.igem.wiki/teams/4683/wiki/measurement/fig7-4.jpg"]}/>
 
-## Results and Analysis
 
+## Results and Analysis
 ### Preliminary Testing
-
 To validate Micro-Q Pro's accuracy, we quantified a range of fluorescein concentrations (0-200 μM) similar to GFP working ranges (de Jonge et al.). Triplicate PCR tubes of 8 were quantified for each of the concentrations below.
 
-| Tube   | Fluorescein Concentration (μM) |
-| ------ | ------------------------------ |
-| Tube 1 | 200                            |
-| Tube 2 | 97                             |
-| Tube 3 | 48                             |
-| Tube 4 | 24.25                          |
-| Tube 5 | 12.3                           |
-| Tube 6 | 6.06                           |
-| Tube 7 | 3.032                          |
-| Tube 8 | 0                              |
+|Tube| Fluorescein Concentration (μM)|
+|-----|-----|
+|Tube 1|200|
+|Tube 2|97|
+|Tube 3|48|
+|Tube 4|24.25|
+|Tube 5|12.3|
+|Tube 6|6.06|
+|Tube 7|3.032|
+|Tube 8|0|
+
 
 Initial comparisons of raw relative fluorescence values (RFU) from Micro-Q Pro, and a plate reader revealed a logarithmic relationship, rather than the expected linear correlation between fluorescence and concentration (Fig. 8)(Itagaki, 2000). After further investigation, we discovered this was due to the camera's reduced sensitivity at higher concentrations, causing oversaturation. This means small perceived camera differences equate to larger fluorescence intensity differences, as proven by the plate reader. To account for this non-linearity, the data requires processing.
 
@@ -135,20 +124,23 @@ Initial comparisons of raw relative fluorescence values (RFU) from Micro-Q Pro,
 
 To linearize our data, we applied a power series regression on all the trials from the 3 triplicates. The curve below achieved the highest R2 value when linearized, so we chose to apply this formula to linearize any further results (See Fig. 9).
 
+
 <Image
   src="https://static.igem.wiki/teams/4683/wiki/measurement/fig9.png"
   caption="Figure 9. Power series regression to linearize Micro-Q values to plate reader values."
 />
 
-Applying the power series regression formula to the previous triplicate test, we get a linear relationship (See Fig. 10).
+
+
+Applying the power series regression formula to the previous triplicate test, we get a linear relationship (See Fig. 10). 
 
 <Image
   src="https://static.igem.wiki/teams/4683/wiki/measurement/fig10.png"
   caption="Figure 10. Micro-Q Pro triplicate results after power series linearization."
 />
 
-### Final Results
 
+### Final Results
 After confirming the linearization of the RFU values from Micro-Q pro, we reran triplicates of the same experiment, and compared the results to the plate reader. Since relative fluorescence units (RFU) are relative, we scaled the output of Micro-Q to match the scale of the output from the plate reader so that they can be compared (see Fig. 11).
 
 <Image
@@ -156,76 +148,40 @@ After confirming the linearization of the RFU values from Micro-Q pro, we reran
   caption="Figure 11. Measurements from plate reader and Micro-Q Pro from 0-200 μM. "
 />
 
+
+
 In order to have an accurate comparison of the data from the plate reader and Micro-Q, we added points at the origin to our data set, and calculated a slope from a linear regression for each measurement device, assuming a y-intercept of 0. The slopes of the data are \~72.1 and \~75.6 from Micro-Q Pro and the Plate reader, respectively, achieving a percent error of -4.629%
 
-## Future Directions & Conclusion
 
-Micro-Q Pro demonstrates promising accuracy in quantifying green fluorescent samples, with no significant differences between Micro-Q Pro and plate reader outputs. This validates the fluorescence measurement capabilities of our hardware device. Although we primarily characterized performance with green fluorophores, preliminary data with red fluorescent materials is also encouraging.
+
+## Future Directions & Conclusion
+Micro-Q Pro demonstrates promising accuracy in quantifying green fluorescent samples, with no significant differences between Micro-Q Pro and plate reader outputs. This validates the fluorescence measurement capabilities of our hardware device. Although we primarily characterized performance with green fluorophores, preliminary data with red fluorescent materials is also encouraging. 
 
 Future iterations could incorporate internal heating pads, enabling fully autonomous fluorescence curves creation during incubations. We also aim to develop an intuitive mobile app for live-streaming fluorescence data and make sample quantification as simple as a click of a button. Additionally, we will resume full characterization across additional spectra once we return to the lab.
 
+
 Micro-Q Pro retains the core functionalities of the original Micro-Q, while dramatically enhancing the ability to efficiently quantify multiple samples across diverse fluorescent spectra—all within a simple $10 device.
 
 As characterization of Micro-Q Pro continues on different fluorophores, Micro-Q Pro makes robust fluorescence quantification widely accessible regardless of financial limitations. Ultimately, this technology enables portable, efficient point-of-care diagnostic testing our miRNA biosensors and other fluorescence-based assays.
 
 ## References
 
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-  fluorescence enabled bioimaging using a model system. PloS one.
-  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703511/
-</Reference>
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-</Reference>
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-  Vivo.” Journal of Experimental Botany, vol. 68, no. 7, 1 Mar. 2017, pp.
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-  performance of low-cost fluorometers. Sensors (Basel, Switzerland).
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-</Reference>
+<Reference>Arppe, R., Carro-Temboury, M. R., Hempel, C., Vosch, T., & Just Sørensen, T. (2017, November 27). Investigating dye performance and crosstalk in fluorescence enabled bioimaging using a model system. PloS one. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703511/  </Reference>
+
+<Reference>Bio-Rad. (n.d.-b). Fluorophores for Violet (405 nm) laser: Bio-Rad. Fluorophores for the Violet (405 nm) Laser. https://www.bio-rad-antibodies.com/flow-cytometry-violet-laser-fluorophores.html </Reference>
+
+<Reference>Chu, J. (2019, September 12). MIT engineers develop “blackest black” material to date. MIT News | Massachusetts Institute of Technology. https://news.mit.edu/2019/blackest-black-material-cnt-0913</Reference>
+
+<Reference>Dondelinger, R. M. (2011, March 1). Spectrophotometers. Allen Press. https://meridian.allenpress.com/bit/article/45/2/139/142090/Spectrophotometers</Reference>
+
+<Reference>De Jonge, Jennifer, et al. “Salicylic Acid Interferes with GFP Fluorescence in Vivo.” Journal of Experimental Botany, vol. 68, no. 7, 1 Mar. 2017, pp. 1689–1696, www.ncbi.nlm.nih.gov/pmc/articles/PMC5441896 /, https://doi.org/10.1093/jxb/erx031.  Accessed 17 Oct. 2022.</Reference>
+
+<Reference>Hixson, J. L., & Ward, A. S. (2022, March 17). Hardware selection and performance of low-cost fluorometers. Sensors (Basel, Switzerland). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954410/#:~:text=Modern%20laboratory%20fluorometers%20range%20in,like%20uranine%20(Table%201).</Reference>
+
+<Reference>Itagaki, H. “Chapter 3 - Fluorescence Spectroscopy.” ScienceDirect, Academic Press, 1 Jan. 2000, www.sciencedirect.com/science/article/abs/pii/B978008050612850009X. </Reference>
+
+<Reference>NHS. (2023, January 30). What happens -Angiography. NHS choices. https://www.nhs.uk/conditions/angiography/what-happens/#:~:text=After%20angiography&text=It%20may%20be%20possible%20to,available%20for%20a%20few%20weeks </Reference>
+
+<Reference>Quantitative analysis of full spectrum LEDs for high quality lighting. (2018, October 1). IEEE Conference Publication | IEEE Xplore. https://ieeexplore.ieee.org/abstract/document/8587355 </Reference>
+
+<Reference>Richard N. Fogoros, M. (2023, February 3). Echocardiogram: Uses, side effects, procedure, results. Verywell Health. https://www.verywellhealth.com/the-echocardiogram-1745246#:~:text=The%20results%20of%20your%20echo,you%20to%20receive%20the%20report </Reference>