From 7d265b57e04e0545dac730e75f457fe473fe8939 Mon Sep 17 00:00:00 2001 From: Xiaotian Zhang <410473713@qq.com> Date: Thu, 12 Oct 2023 15:50:55 +0000 Subject: [PATCH] Update file protocol.html --- wiki/pages/protocol.html | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/wiki/pages/protocol.html b/wiki/pages/protocol.html index 4021842..07196d5 100644 --- a/wiki/pages/protocol.html +++ b/wiki/pages/protocol.html @@ -935,6 +935,7 @@ header{ <br><br>     2. pL2R digestion (Digestion of pL2R with BsaI generated a linearized plasmid having protruding repeat sequences of 4 nt at both ends) <br> + <br>         System: 50 µL <br>         pL2R: 2 µg @@ -950,7 +951,7 @@ header{ <br><br>     3.gRNA annealing (The spacer fragments are designed to carry 4 nt protruding ends complementary to those in the linearized pL2R) <br> - + <br>         System: 10 μL <br>         Primer F/R: 1 μL -- GitLab