diff --git a/wiki/pages/protocol.html b/wiki/pages/protocol.html index 4021842a2bacaa306fcd1b2f88fb6ed82e70b61b..07196d5067c9f7806e13716b6d573c77555cc234 100644 --- a/wiki/pages/protocol.html +++ b/wiki/pages/protocol.html @@ -935,6 +935,7 @@ header{ <br><br>     2. pL2R digestion (Digestion of pL2R with BsaI generated a linearized plasmid having protruding repeat sequences of 4 nt at both ends) <br> + <br>         System: 50 µL <br>         pL2R: 2 µg @@ -950,7 +951,7 @@ header{ <br><br>     3.gRNA annealing (The spacer fragments are designed to carry 4 nt protruding ends complementary to those in the linearized pL2R) <br> - + <br>         System: 10 μL <br>         Primer F/R: 1 μL