{% extends "layout.html" %} {% block title %}Contribution{% endblock %} {% block page_content %}
Soybeans (Glycine max) are a globally important source of livestock food, biofuels, oils, and direct food products. Despite soybeans comprising 50% of all bioengineered crops1, there have been no iGEM teams who have bioengineered Glycine max. We think beans are the best, and hope to see future iGEM teams fully utilize this mighty plant to its full potential. As our contribution to iGEM and its future teams, we have established a protocol for agrobacterium-mediated plant transformation.
Agrobacterium (A. tumefaciens) is much tricker to work with than E. coli, and traditional agrobacterium-mediated tissue cultures of plants involve weeks of subculturing on many different media. This attached protocol is an improvement upon many others, especially for groups with time sensitive needs such as iGEM teams. It is particularly engineered to work with EHA 105s and soybean cultivars Williams 82 and Maverick.
A simplified version of this protocol is also available on our Experiments page.
OD 600 Reading