From 75c25854a58b09e21a5bd155ed062264189ff935 Mon Sep 17 00:00:00 2001
From: Devmc <dev.mcrf@qq.com>
Date: Fri, 9 Sep 2022 15:29:44 +0800
Subject: [PATCH] model
---
static/utils.css | 4 ++
wiki/pages/model.html | 103 +++++++++++++++++++++++++++++++++++++++++-
2 files changed, 105 insertions(+), 2 deletions(-)
diff --git a/static/utils.css b/static/utils.css
index f6842fc..07960c8 100644
--- a/static/utils.css
+++ b/static/utils.css
@@ -236,6 +236,10 @@
color: #279704;
}
+.c-purple {
+ color: #A020F0;
+}
+
/* ========================
* container
* =======================*/
diff --git a/wiki/pages/model.html b/wiki/pages/model.html
index 05ca803..c1d66dc 100644
--- a/wiki/pages/model.html
+++ b/wiki/pages/model.html
@@ -10,8 +10,107 @@
<h1 class="content-header2">Modeling</h1>
<section>
- <h2></h2>
- <p></p>
+ <p>
+ The numerical model was applied to investigate the relationship between the concentration concentration of DNA
+ and the fluorescence value of the plasmids transferred into gene fragments, including pUC57-cagA, pUC57-inVA,
+ pUC57-ipaH and Puc57-16S. Table 1 presents the experimental data of the fluorescence values of the four
+ plasmids and concentration of DNA.
+ </p>
+ <div class="table-container">
+ <span class="figure rw-75">
+ Table 1 presents the experimental data of the fluorescence values of the four plasmids and concentration of
+ DNA.
+ </span>
+ <table class="rw-65 mx-auto text-center">
+ <tbody>
+ <tr>
+ <th>Plasmids DNA</th>
+ <th>0</th>
+ <th>1</th>
+ <th>2</th>
+ <th>4</th>
+ </tr>
+ <tr>
+ <td>pUC57-cagA</td>
+ <td>2635808</td>
+ <td>2929973</td>
+ <td>2943897</td>
+ <td>3593478</td>
+ </tr>
+ <tr>
+ <td>pUC57-inVA</td>
+ <td>2875848</td>
+ <td>3142023</td>
+ <td>3833321</td>
+ <td>5684118</td>
+ </tr>
+ <tr>
+ <td>pUC57-ipaH</td>
+ <td>2953330</td>
+ <td>2952940</td>
+ <td>3667676</td>
+ <td>4101707</td>
+ </tr>
+ <tr>
+ <td>Puc57-16S</td>
+ <td>2316060</td>
+ <td>2381208</td>
+ <td>3289690</td>
+ <td>3759060</td>
+ </tr>
+ </tbody>
+ </table>
+ </div>
+ </section>
+
+ <section>
+ <h2>Modeling steps:</h2>
+ <p>
+ 1. Analyze the scatter plot of 4 groups of samples; <br>
+ 2. Select the appropriate model <br>
+ 3. Analyze the comparison curve.Model : Hermite interpolation model <br>
+ (Emmett interpolation model <br>
+ The specific procedures are as follows: <br>
+ </p>
+ <pre class="m-t-2" style="font-size: 18px !important;">
+DNA_0=[0 1 2 4];
+PUC57_cagA_0=[2635808 2929973 2943897 3593478];
+PUC57_inVA_0=[2875848 3142023 3833321 5684118];
+PUC57_ipaH_0=[2953330 2952940 3667676 4101707];
+PUC57_16S_0=[2316060 2381208 3289690 3759060];
+DNA=[0:0.05:4.1];
+PUC57_cagA=interp1(DNA_0,PUC57_cagA_0,DNA,<font class="c-purple">'pchip'</font>);
+PUC57_inVA=interp1(DNA_0,PUC57_inVA_0,DNA,<font class="c-purple">'pchip'</font>);
+PUC57_ipaH=interp1(DNA_0,PUC57_ipaH_0,DNA,<font class="c-purple">'pchip'</font>);
+PUC57_16S=interp1(DNA_0,PUC57_16S_0,DNA,<font class="c-purple">'pchip'</font>);
+subplot(2,2,1)
+plot(DNA,PUC57_cagA,<font class="c-purple">'b'</font>,DNA_0,PUC57_cagA_0,<font class="c-purple">'r*'</font>,<font
+ class="c-purple">'LineWidth'</font>,2)
+subplot(2,2,2)
+plot(DNA,PUC57_inVA,<font class="c-purple">'b'</font>,DNA_0,PUC57_inVA_0,<font class="c-purple">'r*'</font>,<font
+ class="c-purple">'LineWidth'</font>,2)
+subplot(2,2,3)
+plot(DNA,PUC57_ipaH,<font class="c-purple">'b'</font>,DNA_0,PUC57_ipaH_0,<font class="c-purple">'r*'</font>,<font
+ class="c-purple">'LineWidth'</font>,2)
+subplot(2,2,4)
+plot(DNA,PUC57_16S,<font class="c-purple">'b'</font>,DNA_0,PUC57_16S_0,<font class="c-purple">'r*'</font>,<font
+ class="c-purple">'LineWidth'</font>,2)
+ </pre>
+ <div class="imager">
+ <img class="rw-100" src="https://static.igem.wiki/teams/4304/wiki/modeling/t-ykpao-modeling-01.jpg" alt="">
+ <span class="figure rw-65">
+ Figure 1. The relationship between the concentration concentration of DNA and the fluorescence value of the
+ four plasmids.
+ </span>
+ </div>
+ <p>
+ The fluorescence value of pUC57-cagA increased slowly and then increased linearly with increasing DNA
+ concentration. The fluorescence value of pUC57-inVA increased linearly with increasing DNA concentration.
+ However, the fluorescence value of pUC57-ipaH and Puc57-16S increased first, and then stabilized with
+ increasing DNA concentration.The model was used to predict the relationship between the fluorescence value and
+ the DNA content of pathogenic bacteria in the sample.It has important biological significance for studying
+ pathogenic bacteria.
+ </p>
</section>
</div>
</div>
--
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