diff --git a/static/utils.css b/static/utils.css index f6842fc3fea0beafc6c85cee69f3d598b8f6465a..07960c8cc8eab9d97c91163bdd7cf70be428db71 100644 --- a/static/utils.css +++ b/static/utils.css @@ -236,6 +236,10 @@ color: #279704; } +.c-purple { + color: #A020F0; +} + /* ======================== * container * =======================*/ diff --git a/wiki/pages/model.html b/wiki/pages/model.html index 05ca803836616ea3c8fb32bcf4ed50d9413fe67d..c1d66dc1eb934481c235fe2e5d0740f0002b4507 100644 --- a/wiki/pages/model.html +++ b/wiki/pages/model.html @@ -10,8 +10,107 @@ <h1 class="content-header2">Modeling</h1> <section> - <h2></h2> - <p></p> + <p> + The numerical model was applied to investigate the relationship between the concentration concentration of DNA + and the fluorescence value of the plasmids transferred into gene fragments, including pUC57-cagA, pUC57-inVA, + pUC57-ipaH and Puc57-16S. Table 1 presents the experimental data of the fluorescence values of the four + plasmids and concentration of DNA. + </p> + <div class="table-container"> + <span class="figure rw-75"> + Table 1 presents the experimental data of the fluorescence values of the four plasmids and concentration of + DNA. + </span> + <table class="rw-65 mx-auto text-center"> + <tbody> + <tr> + <th>Plasmids DNA</th> + <th>0</th> + <th>1</th> + <th>2</th> + <th>4</th> + </tr> + <tr> + <td>pUC57-cagA</td> + <td>2635808</td> + <td>2929973</td> + <td>2943897</td> + <td>3593478</td> + </tr> + <tr> + <td>pUC57-inVA</td> + <td>2875848</td> + <td>3142023</td> + <td>3833321</td> + <td>5684118</td> + </tr> + <tr> + <td>pUC57-ipaH</td> + <td>2953330</td> + <td>2952940</td> + <td>3667676</td> + <td>4101707</td> + </tr> + <tr> + <td>Puc57-16S</td> + <td>2316060</td> + <td>2381208</td> + <td>3289690</td> + <td>3759060</td> + </tr> + </tbody> + </table> + </div> + </section> + + <section> + <h2>Modeling steps:</h2> + <p> + 1. Analyze the scatter plot of 4 groups of samples; <br> + 2. Select the appropriate model <br> + 3. Analyze the comparison curve.Model : Hermite interpolation model <br> + (Emmett interpolation model <br> + The specific procedures are as follows: <br> + </p> + <pre class="m-t-2" style="font-size: 18px !important;"> +DNA_0=[0 1 2 4]; +PUC57_cagA_0=[2635808 2929973 2943897 3593478]; +PUC57_inVA_0=[2875848 3142023 3833321 5684118]; +PUC57_ipaH_0=[2953330 2952940 3667676 4101707]; +PUC57_16S_0=[2316060 2381208 3289690 3759060]; +DNA=[0:0.05:4.1]; +PUC57_cagA=interp1(DNA_0,PUC57_cagA_0,DNA,<font class="c-purple">'pchip'</font>); +PUC57_inVA=interp1(DNA_0,PUC57_inVA_0,DNA,<font class="c-purple">'pchip'</font>); +PUC57_ipaH=interp1(DNA_0,PUC57_ipaH_0,DNA,<font class="c-purple">'pchip'</font>); +PUC57_16S=interp1(DNA_0,PUC57_16S_0,DNA,<font class="c-purple">'pchip'</font>); +subplot(2,2,1) +plot(DNA,PUC57_cagA,<font class="c-purple">'b'</font>,DNA_0,PUC57_cagA_0,<font class="c-purple">'r*'</font>,<font + class="c-purple">'LineWidth'</font>,2) +subplot(2,2,2) +plot(DNA,PUC57_inVA,<font class="c-purple">'b'</font>,DNA_0,PUC57_inVA_0,<font class="c-purple">'r*'</font>,<font + class="c-purple">'LineWidth'</font>,2) +subplot(2,2,3) +plot(DNA,PUC57_ipaH,<font class="c-purple">'b'</font>,DNA_0,PUC57_ipaH_0,<font class="c-purple">'r*'</font>,<font + class="c-purple">'LineWidth'</font>,2) +subplot(2,2,4) +plot(DNA,PUC57_16S,<font class="c-purple">'b'</font>,DNA_0,PUC57_16S_0,<font class="c-purple">'r*'</font>,<font + class="c-purple">'LineWidth'</font>,2) + </pre> + <div class="imager"> + <img class="rw-100" src="https://static.igem.wiki/teams/4304/wiki/modeling/t-ykpao-modeling-01.jpg" alt=""> + <span class="figure rw-65"> + Figure 1. The relationship between the concentration concentration of DNA and the fluorescence value of the + four plasmids. + </span> + </div> + <p> + The fluorescence value of pUC57-cagA increased slowly and then increased linearly with increasing DNA + concentration. The fluorescence value of pUC57-inVA increased linearly with increasing DNA concentration. + However, the fluorescence value of pUC57-ipaH and Puc57-16S increased first, and then stabilized with + increasing DNA concentration.The model was used to predict the relationship between the fluorescence value and + the DNA content of pathogenic bacteria in the sample.It has important biological significance for studying + pathogenic bacteria. + </p> </section> </div> </div>