From 66267a74d82ef0e7f2eebc3337883ee5538d5615 Mon Sep 17 00:00:00 2001
From: Devmc <dev.mcrf@qq.com>
Date: Wed, 14 Sep 2022 21:13:11 +0800
Subject: [PATCH] notebook
---
static/utils.css | 191 +++++++++++++++++++++++++++++++++++++++
wiki/pages/notebook.html | 118 +++++++++++++++++++++++-
wiki/pages/safety.html | 77 +++++++++++++++-
3 files changed, 382 insertions(+), 4 deletions(-)
diff --git a/static/utils.css b/static/utils.css
index 07960c8..f93e715 100644
--- a/static/utils.css
+++ b/static/utils.css
@@ -96,6 +96,97 @@
margin-bottom: 5rem !important;
}
+
+/*m-s*/
+.m-s-0 {
+ margin-left: 0 !important;
+}
+
+.m-s-1 {
+ margin-left: 0.5rem !important;
+}
+
+.m-s-2 {
+ margin-left: 1rem !important;
+}
+
+.m-s-3 {
+ margin-left: 1.5rem !important;
+}
+
+.m-s-4 {
+ margin-left: 2rem !important;
+}
+
+.m-s-5 {
+ margin-left: 2.5rem !important;
+}
+
+.m-s-6 {
+ margin-left: 3rem !important;
+}
+
+.m-s-7 {
+ margin-left: 3.5rem !important;
+}
+
+.m-s-8 {
+ margin-left: 4rem !important;
+}
+
+.m-s-9 {
+ margin-left: 4.5rem !important;
+}
+
+.m-s-10 {
+ margin-left: 5rem !important;
+}
+
+/*m-e*/
+.m-e-0 {
+ margin-right: 0 !important;
+}
+
+.m-e-1 {
+ margin-right: 0.5rem !important;
+}
+
+.m-e-2 {
+ margin-right: 1rem !important;
+}
+
+.m-e-3 {
+ margin-right: 1.5rem !important;
+}
+
+.m-e-4 {
+ margin-right: 2rem !important;
+}
+
+.m-e-5 {
+ margin-right: 2.5rem !important;
+}
+
+.m-e-6 {
+ margin-right: 3rem !important;
+}
+
+.m-e-7 {
+ margin-right: 3.5rem !important;
+}
+
+.m-e-8 {
+ margin-right: 4rem !important;
+}
+
+.m-e-9 {
+ margin-right: 4.5rem !important;
+}
+
+.m-e-10 {
+ margin-right: 5rem !important;
+}
+
/*p*/
.p-0 {
padding: 0 !important;
@@ -191,6 +282,96 @@
padding-bottom: 5rem !important;
}
+/*p-s*/
+.p-s-0 {
+ padding-left: 0 !important;
+}
+
+.p-s-1 {
+ padding-left: 0.5rem !important;
+}
+
+.p-s-2 {
+ padding-left: 1rem !important;
+}
+
+.p-s-3 {
+ padding-left: 1.5rem !important;
+}
+
+.p-s-4 {
+ padding-left: 2rem !important;
+}
+
+.p-s-5 {
+ padding-left: 2.5rem !important;
+}
+
+.p-s-6 {
+ padding-left: 3rem !important;
+}
+
+.p-s-7 {
+ padding-left: 3.5rem !important;
+}
+
+.p-s-8 {
+ padding-left: 4rem !important;
+}
+
+.p-s-9 {
+ padding-left: 4.5rem !important;
+}
+
+.p-s-10 {
+ padding-left: 5rem !important;
+}
+
+/*p-e*/
+.p-e-0 {
+ padding-right: 0 !important;
+}
+
+.p-e-1 {
+ padding-right: 0.5rem !important;
+}
+
+.p-e-2 {
+ padding-right: 1rem !important;
+}
+
+.p-e-3 {
+ padding-right: 1.5rem !important;
+}
+
+.p-e-4 {
+ padding-right: 2rem !important;
+}
+
+.p-e-5 {
+ padding-right: 2.5rem !important;
+}
+
+.p-e-6 {
+ padding-right: 3rem !important;
+}
+
+.p-e-7 {
+ padding-right: 3.5rem !important;
+}
+
+.p-e-8 {
+ padding-right: 4rem !important;
+}
+
+.p-e-9 {
+ padding-right: 4.5rem !important;
+}
+
+.p-e-10 {
+ padding-right: 5rem !important;
+}
+
/*response width*/
.rw-100 {
width: 100% !important;
@@ -682,6 +863,16 @@ section > embed {
padding-left: 1rem;
}
+.article ul.l-start-2,
+.article ol.l-start-2 {
+ padding-left: 2rem;
+}
+
+.article ul.l-start-3,
+.article ol.l-start-3 {
+ padding-left: 3rem;
+}
+
.article ul.l-none,
.article ol.l-none {
list-style-type: none;
diff --git a/wiki/pages/notebook.html b/wiki/pages/notebook.html
index 2d3e747..5755b70 100644
--- a/wiki/pages/notebook.html
+++ b/wiki/pages/notebook.html
@@ -10,8 +10,122 @@
<h1 class="content-header2">Notebook</h1>
<section>
- <h2></h2>
- <p></p>
+ <h2 class="c-green">August</h2>
+
+ <section>
+ <h3>11<sup>th</sup></h3>
+ <p>- Culture <i>E. coli</i></p>
+
+ <div class="imager">
+ <img class="rw-35" src="https://static.igem.wiki/teams/4304/wiki/notebook/t-ykpao-notebook-01.jpg" alt="">
+ </div>
+ </section>
+
+ <section>
+ <br>
+ <h3>12<sup>th</sup></h3>
+ <p>- Cas12a protein expression:</p>
+ <ul>
+ <li>Addition of IPTG</li>
+ <li>Test OD<sub>600</sub> value of pET-28a integrated <i>E. coli</i> BL21(DE3)</li>
+ </ul>
+ <p>- pUC57 plasmid extraction:</p>
+ <ul>
+ <li>Measure the concentration using Nanodrop</li>
+ </ul>
+ <p>PCR of cagA, ipaH, invA, 16S_ pUC57 plasmids</p>
+ <div class="imager">
+ <img class="rw-100" src="https://static.igem.wiki/teams/4304/wiki/notebook/t-ykpao-notebook-02.jpg" alt="">
+ </div>
+ </section>
+
+ <section>
+ <br>
+ <h3>13<sup>th</sup></h3>
+ <p>- Cas12a protein extraction:</p>
+ <ul>
+ <li>Ultrasound lysis</li>
+ <li>Nickel affinity purification</li>
+ </ul>
+ <p>- Gel electrophoresis of cagA, ipaH, invA, and 16S plasmids:</p>
+ <ul>
+ <li>Extraction of target DNA fragments</li>
+ <li>Measure the concentration using Nanodrop</li>
+ </ul>
+
+ <div class="imager">
+ <img class="rw-35" src="https://static.igem.wiki/teams/4304/wiki/notebook/t-ykpao-notebook-03.png" alt="">
+ </div>
+ </section>
+
+ <section>
+ <br>
+ <h3>14<sup>th</sup></h3>
+ <p>- Cas12a protein electrophoresis,SDS-PAGE</p>
+ <div class="imager">
+ <img class="rw-65" src="https://static.igem.wiki/teams/4304/wiki/notebook/t-ykpao-notebook-04.jpg" alt="">
+ </div>
+ <p class="m-t-2">- cagA, ipaH, invA, and 16S DNA in vitro transcription</p>
+ <p>- sgRNA purification</p>
+ <div class="imager">
+ <img class="rw-65" src="https://static.igem.wiki/teams/4304/wiki/notebook/t-ykpao-notebook-05.jpg" alt="">
+ </div>
+ </section>
+
+ <section>
+ <br>
+ <h3>15<sup>th</sup></h3>
+ <p>- Incubation of sgRNA and Cas12a proteins</p>
+ <p>- Test the reaction system using (cagA, ipaH, invA, and 16S) pUC57 plasmid</p>
+ <ul>
+ <li>ssDNA was included in the system and the result will be based on fluorescent intensity</li>
+ </ul>
+ <p>- Test the reaction system using oligo DNA (cagA, ipaH, invA, and 16S)</p>
+ <ul>
+ <li>verify using gel electrophoresis after PCR</li>
+ </ul>
+ <div class="imager">
+ <img class="rw-65" src="https://static.igem.wiki/teams/4304/wiki/notebook/t-ykpao-notebook-06.jpg" alt="">
+ </div>
+ </section>
+
+ <section>
+ <br>
+ <h3>16<sup>th</sup></h3>
+ <p>- Incubation of sgRNA and Cas12a proteins</p>
+ <p>- Test reaction system using oligo DNA (cagA, ipaH, invA, and 16S)</p>
+ <ul>
+ <li>prove using gel electrophoresis after PCR</li>
+ </ul>
+ <p>- Test reaction system using (cagA, ipaH, invA, and 16S) pUC57 plasmid</p>
+ <ul>
+ <li>ssDNA was included in the system and the result will be based on fluorescent intensity</li>
+ </ul>
+ <div class="imager">
+ <img class="rw-100" src="https://static.igem.wiki/teams/4304/wiki/notebook/t-ykpao-notebook-07.jpg" alt="">
+ </div>
+ </section>
+
+ <section>
+ <br>
+ <h3>17<sup>th</sup></h3>
+ <p>- Test resulting system (cagA, ipaH, invA, and 16S) pUC57 plasmid </p>
+ <ul>
+ <li>ssDNA was included in the system and the result will be based on fluorescent intensity detected by multiscan ascent</li>
+ </ul>
+ <div class="imager">
+ <img class="rw-65" src="https://static.igem.wiki/teams/4304/wiki/notebook/t-ykpao-notebook-08.jpg" alt="">
+ </div>
+ <p class="m-t-2">- Test the reaction system using bacteria culture</p>
+ <ul>
+ <li>Bacteria lysis</li>
+ <li>Test PCR results using gel electrophoresis</li>
+ <li>Add ssDNA into the system and test using multiscan ascent</li>
+ </ul>
+ <div class="imager">
+ <img class="rw-50" src="https://static.igem.wiki/teams/4304/wiki/notebook/t-ykpao-notebook-09.png" alt="">
+ </div>
+ </section>
</section>
</div>
</div>
diff --git a/wiki/pages/safety.html b/wiki/pages/safety.html
index 90fc05e..8d58c3f 100644
--- a/wiki/pages/safety.html
+++ b/wiki/pages/safety.html
@@ -10,8 +10,81 @@
<h1 class="content-header2">Safety</h1>
<section>
- <h2></h2>
- <p></p>
+ <h2 class="c-green">1. General Lab safety</h2>
+ <ul class="l-top-05 l-start-3">
+ <li>Wear gloves and lab coats</li>
+ <li>Wear shoes with close ends</li>
+ <li>No eating, drinking or running in Lab</li>
+ <li>Tie up long hair</li>
+ <li>Dispose biological wastes in specific bins</li>
+ <li>Carry our experiments with poisonous or pungent gases in a fume cupboard</li>
+ <li>Clean up the lab before leaving</li>
+ </ul>
+ </section>
+
+ <section>
+ <h2 class="c-green">2. Experimental safety</h2>
+
+ <section>
+ <h3 class="upper p-s-3">Operation of the clean bench/fume cupboard:</h3>
+ <ul class="l-top-05 l-start-3">
+ <li>Sterilize the cabinet by opening UV light before usage for 30 minutes</li>
+ <li>Do not stare directly at the UV light</li>
+ <li>Open laminar airflow before usage</li>
+ <li>Sterilize all apparatus over the Bunsen burner before using them</li>
+ <li>All caps and lids should be placed with their bottoms up, and sterilized above the flame before usage
+ </li>
+ <li>Spray alcohol and scrub hands</li>
+ </ul>
+ </section>
+
+ <section>
+ <h3 class="p-s-3">Operation using a pipette</h3>
+ <ul class="l-top-05 l-start-3">
+ <li>The non-disposable part of the pipette shouldn’t be in contact with any reagents to prevent
+ contamination
+ </li>
+ </ul>
+ </section>
+
+ <section>
+ <h3 class="p-s-3">Operations using Coomassie brilliant blue dye</h3>
+ <ul class="l-top-05 l-start-3">
+ <li>Carry out all procedures in a fume cupboard</li>
+ </ul>
+ </section>
+
+ <section>
+ <h3 class="p-s-3">Operations regarding gel electrophoresis</h3>
+ <ul class="l-top-05 l-start-3">
+ <li>Wear gloves as the gel is poisonous</li>
+ </ul>
+ </section>
+ </section>
+
+ <section>
+ <h2 class="c-green">3. Micro-organism safety</h2>
+ <p>
+ Our team only uses one type of bacteria in the experiment: E. coli BL21(DE3), a non-pathogenic strain of
+ microorganisms. This is due to the biohazard applications <i>H.pylori</i>, <i>S. Typhimurium</i>, and <i>S.
+ flexneri</i> pose.
+ However, in consideration of the environmental and ecological issues, all substances contaminated by <i>E.
+ coli</i>
+ BL21(DE3) will be sterilized before disposal and will be discarded in the biohazardous bin.
+ </p>
+ <p>
+ All enzymes and genetic materials (including cagA_ pUC57, 16S_ pUC57, ipaH_ pUC57, invA_ pUC57, FnCas-12a_
+ pET-28a, and so on) pose minimum risks to our health. Furthermore, all lab apparatus is uncontaminated and was
+ sterilized or cleaned before use.
+ </p>
+ </section>
+
+ <section>
+ <h2 class="c-green">4. Ethics</h2>
+ <p>
+ Our experiment doesn’t consist of any human or animal parts or genes. And all genetic modifications that were
+ established in our process are limited to research purposes.
+ </p>
</section>
</div>
</div>
--
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