1. Plate all 5 E. coli strains on the LB medium plates, respectively (Cas12a strain on the plate with Kana antibiotics and the other 4 plates with Ampicillin antibiotics)
  2. incubate overnight and use a pipette to pick a single colony of E. coli
  3. inoculate single colonies in 3ml LB. A total of 5 flasks will be prepared, incubate overnight at 37℃, 200rpm.