1. Design primer using Snapgene with Helicobacter pylori, Salmonella Typhimurium, and Shigella Flexner’s genome as templates. 10 templates will be designed in total. See the chart below for more details:
    Bacteria Origin Number of primers
    Primer-F Primer-R Target Sequence(s)
    H. Pylori 2 2 cagA, 16S
    S. Typhimurium 1 1 invA
    S. flexneri 1 1 ipaH
    Francisella tularensis subsp. novicida FTG 1 1 FnCas12a

See primer sequences as attached.

(BBa_K4304021; BBa_K4304022; BBa_K4304023; BBa_K4304024; BBa_K4304025; BBa_K4304026; BBa_K4304027; BBa_K4304028; BBa_K4304029; BBa_K4304030)