Primer sequence:
16S:
F: CCGTAAGGAGGAGGAAGGT
R: GCAACATGGCTGATTTGCG
FIP: TTGCTTCTCTTTTGTGCACCCCCAGTCAAGTCATCATGGCCCTT
RIP: ACTGCGAAGTGGGGAGCCAATCTTTGCTTCATGCAGGCAGTT
cagA:
F: GATTTAATCAACAAAGACGCTC
R: TCTGCTTTTTCTTTGTCATCAGG
FIP: CCAACTTGTGAAAATTCGGTAACGGTAGAATCTTCCACAAAGAGCT
RIP: GTGTCCCATCAAAACGATCCGTAGGGGGTTGTATGgTATgTTCC
invA:
F:GGCGATATTGGTGTTTATGGGG
R: AACGATAAACTGGACCACGG
FIP: GACGACTGGTACTGATCGATAGTTTTTCAACGTTTCCTGCGG
BIP: CCGGTGAAATTATCGCCACACAAAACCCACCGCCAGG
ipaH:
F:GCTTCGACAGCAGTCTTT
R: GGCCAGGTAGACTTCTATCT
FIP: CCTTCTGATGCCTGATGGACCCACTGAGAGCTGTGAGGA
BIP: ATAATGATACCGGCGCTCTGCCCTCCAGAATTTCGAGGC
Procedure:
Reagent | 25μl Reaction system | Final concentration |
---|---|---|
2×Lamp Master Mix | 12.5 μl | 1× |
FIP (10μM) | 2μl | 0.8μM |
BIP (10μM) | 2μl | 0.8μM |
F3 (10μM) | 0.5μl | 0.2μM |
B3 (10μM) | 0.5μl | 0.2μM |
Template DNA | 1.5μl | 1-4 ng/μl |
DNA polymerase | 0.5μl | 0.16 U/μl |
Sterilized ddH2O | 5.5μl | - |