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Overview


All these parts have the same function of defluorinating. Both haloacid dehalogenase (HAcD) and fluoroacetate dehalogenase (FAcD) are enzymes that catalyze the cleavage of the C-F bond. In our project this was used to cleave the C-F bonds that make PFOA extremely hard to degrade, but it can also be applied to a variety of environmental conditions and other projects where either the C-F bond needs to be cleaved.

Table


Part Name Short Description
BBa_K4191004 HAcD CDS, derived from X. autotrophicus, codon optimized for E. coli
BBa_K4191001 FAcD CDS, derived from R. palustris, codon optimized for E. coli
BBa_K4191002 PJ23119 + RB0034 + FAcD + TB0010
BBa_K4191003 PJ23119 + RB0034 + HAcD + TB0010
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