From 3d21d650c7f8842ceed4509991d821af095cbf59 Mon Sep 17 00:00:00 2001
From: Xiyue Ai <1158482239@qq.com>
Date: Thu, 13 Oct 2022 14:30:50 +0000
Subject: [PATCH] Update wiki/pages/notebook.html
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<p>
<br>
<b>2022-07-01~2022-07-07</b>   <br><br>We designed and improved the following experiments:
- Chlamydomonas reinhardtii culture experiment, paddle plate conservation and conservation recovery
+ <i>Chlamydomonas reinhardtii</i> culture experiment, paddle plate conservation and conservation recovery
amplification experiment, TEA electrophoresis experiment, electro-transformation and glass bead
transformation experiment, OD measurement by Thermo Fisher ELISA, and plasmid extraction protocol.<br>
- We reviewed the literature to identify genes and pathways in the metabolic pathway of Chlamydomonas
- reinhardtii that can significantly affect its lipid production. <br>
- We did liquid and solid cultures of Chlamydomonas reinhardtii and finalized six different stress conditions
- to be performed on Chlamydomonas
- reinhardtii: N stress, Fe stress, salt stress, N+S stress, N+P stress, and S+P stress. <br>
- We found that our cultivation of Chlamydomonas reinhardtii was contaminated by stray bacteria, so we
+ We reviewed the literature to identify genes and pathways in the metabolic pathway of <i>Chlamydomonas reinhardtii</i> that can significantly affect its lipid production. <br>
+ We did liquid and solid cultures of <i>Chlamydomonas reinhardtii</i> and finalized six different stress conditions
+ to be performed on <i>Chlamydomonas reinhardtii</i>: N stress, Fe stress, salt stress, N+S stress, N+P stress, and S+P stress. <br>
+ We found that our cultivation of <i>Chlamydomonas reinhardtii</i> was contaminated by stray bacteria, so we
disinfected our laboratory and light incubator. <br><br>
<b>2022-07-08~2022-07-14</b>  <br><br> We have designed and improved the protocol for lipid
extraction.<br>
We reviewed the literature and identified seven genes to be knocked out by the Crispr-cas9 system to promote
- lipid production in Chlamydomonas reinhardtii: LACS1, LACS2, DTH1, CHT7, PEPC1, ACX2, PLA2, and ICL1.<br>
- We designed protocols for six stress conditions of Chlamydomonas reinhardtii.<br>
+ lipid production in <i>Chlamydomonas reinhardtii</i>: LACS1, LACS2, DTH1, CHT7, PEPC1, ACX2, PLA2, and ICL1.<br>
+ We designed protocols for six stress conditions of <i>Chlamydomonas reinhardtii</i>.<br>
Under the guidance of our advisor Xu Tang we started to build the CRISPR-Cas9 system plasmid.<br><br>
<b>2022-07-15~2022-07-21</b>  <br><br> We designed experiments to measure the OD values and cell
- numbers at 750 nm of Chlamydomonas reinhardtii at different gradient dilutions under normal culture
- conditions, and worked with modeling members to establish the growth curve of Chlamydomonas reinhardtii
+ numbers at 750 nm of <i>Chlamydomonas reinhardtii</i> at different gradient dilutions under normal culture
+ conditions, and worked with modeling members to establish the growth curve of <i>Chlamydomonas reinhardtii</i>
under normal cultivation.<br>
We obtained the plasmid PZHY989 (with fluorescent protein YFP), which was kindly donated by the university
laboratory, and used it to start experimenting with glass bead transformation and electrotransformation
@@ -123,7 +121,7 @@
<br>We Started experimenting with Nile Red staining and kept improving the protocol while observing the
staining results under fluorescence microscope.
<br>We did pre-experiments with six groups of stress conditions. Besides, a method was devised for freezing
- and preserving the seeds of Chlamydomonas reinhardtii.
+ and preserving the seeds of <i>Chlamydomonas reinhardtii</i>.
<br>We improved the treatment conditions in the electrotransformation protocol we learned from others, made
a second attempt, and set up different concentration gradients of screening pressure for thaumatin so as to
find the most suitable conditions for selecting algae.
--
GitLab