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Overview

Throughout the year, we have been trying to obtain antimicrobial peptides from eukaryotic organisms in bacteria. In addition, we have designed constructs to obtain double-stranded siRNAs that can help us reduce the pathogenicity of wilt-causing organisms. On this page you can see the parts we created, the parts we use and the expression cassettes we designed.


Accession number
Description Purpose Diagram Length (pb)
Status
Basic parts
BBa_K4147000 An antimicrobial peptide from Piper colubrinum
(PcOSM)
To have the sequence of the antimicrobial peptide. PcOSM schematic representation 711 In progress
BBa_K4147001 An antimicrobial peptide from Phyllomedusa bicolor that contains a tandem repeat of a chitin binding domai (CBD) as an improvement.
(CBD)2-DrsB1
To improve the antifungal efficiency of dermaseptin in the registry. (CBD)2-DrsB1 schematic representation 711 In progress
BBa_K4147016 siRNA designed to target RXLR gene of P. capsici. To suppress the RXLR gene in P. capsici to decrease its pathogenicity.
Irnaldo waving
18 In progress
BBa_K4147014 Reverse of BBa_K525998 To produce the double-stranded siRNAs. Reverse promoter schematic representation 32 In progress
BBa_K4147015 Reverse of BBa K864600 To produce the double-stranded siRNAs. reverse terminator schematic representation 18 In progress
BBa_K4147006 Disulfide interchange protein (DsbA) from Pseudomonas aeruginosa To enhance the formation of disulfide bridges of antimicrobial peptides by expressing them in E. coli BL21 (DE3). DsbA schematic representation 636 In progress
BBa_K4147017 siRNA against ODC gene To produce the double-stranded siRNAs. reverse terminator schematic representation 18 In progress
BBa_K4147009 MsrA2 antimicrobial protein for purification To produce MsrA2 fused with 6X-Hist tag for purification by metal affinity chromatography Expression cassette schematic representation 117 In progress
Composite parts
BBa_K4147002 Expression cassette for (CBD)2-DrsB1 peptide To produce the antimicrobial peptide (CBD)2-DrsB1 in E. coli BL21 (DE3). Expression cassette schematic representation 1075 Ligated
BBa_K4147003 Expression cassette for PcOSM peptide To produce the antimicrobial peptide PcOSM in E. coli BL21 (DE3). Expression cassette schematic representation 1054 Ligated
BBa_K4147007 Expression cassette for PcOSM peptide with DsbA To produce the antimicrobial peptide PcOSM in E. coli BL21 (DE3) while enhancing disulfide bridge formation. Expression cassette schematic representation 1738 Ligated
BBa_K4147008 Expression cassette for (CBD)2-DrsB1 peptide with DsbA To produce the antimicrobial peptide PcOSM in E. coli BL21 (DE3) while enhancing disulfide bridge formation. Expression cassette schematic representation 1738 Ligated
BBa_K4147010 Expression cassette for mCherry with BBa_R0010 promoter Experiments to improve the mathematical model by measuring the amount of protein produced under this expression construct. Expression cassette schematic representation 1738 Ligated
BBa_K4147011 Expression cassette for mCherry with BBa_R0011 promoter Experiments to improve the mathematical model by measuring the amount of protein produced under this expression construct. Expression cassette schematic representation 1738 Ligated
BBa_K4147004 Expression cassette for DrsB1 construct with lacI promoter To produce the antimicrobial peptide DrsB1 in E. coli BL21 (DE3). Expression cassette schematic representation 481 Ligated
BBa_K4147012 Up part of cassette for siRNA production of RXLR gene for P. capsici Experiments to improve the mathematical model by measuring the amount of protein produced under this expression construct. Expression cassette schematic representation 118 In progress
BBa_K4147013 Down part of cassette for siRNA production Experiments to improve the mathematical model by measuring the amount of protein produced under this expression construct. Expression cassette schematic representation 92 In progress
Existing parts used
BBa_R0011 Hybrid region regulatory promoter. Consists of phage lambda promoter P(L) with cl binding sites replaced by lacO1. Can be repressed by LacI inhibitor and can be induced by IPTG. Express antimicrobial peptides and test our model. Promoter schematic representation 55 Works
BBa_R0010 LacI regulated promoter. It can be induced by IPTG. Express antimicrobial peptides and test our model. Promoter schematic representation 200 Works
BBa_B0032 Weak RBS based on Ron Weiss's thesis Express antimicrobial peptides. RBS schematic representation 13 Works
BBa_B0015 Double terminator Express antimicrobial peptides. Double terminator schematic representation 13 Works
BBa_K525998 T7 promoter and RBS siRNA production.  representation 20 Works
BBa K864600 Double terminator Express antimicrobial peptides. Terminator schematic representation 52 Works
BBa_K2577001 MsrA2 antimicrobial protein To compare the fungal activity of this protein alone compared to that fused with CBD (BBa_R0001). Drsb1 schematic representation 96 In progress


Go to Experiments

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