diff --git a/wiki/pages/communication.html b/wiki/pages/communication.html
index 276e04f1b1082b9078df4cf59724409d702fe878..de05bec00d0e1b5eb7136f34a4f7ae8b4deca476 100644
--- a/wiki/pages/communication.html
+++ b/wiki/pages/communication.html
@@ -41,20 +41,20 @@
<tr>
<td>Chinese shadow puppetry</td>
<td>Children & Elderly & College students</td>
- <td>The elderly are disseminators of traditional culture, so we popularize science through the shadow puppetry that they can easily accept. Children are the inheritors of traditional culture and the future innovators of synthetic biology, so we popularize science and traditioanl culture for them. College students are learners of science, so we perform shadow puppetry in the school sports ground to popularize synthetic biology while spreading traditional culture for them. </td>
+ <td>The elderly are disseminators of traditional culture, so we popularize science through the shadow puppetry which they can easily accept. Children are the inheritors of traditional culture and the future innovators of synthetic biology, so we popularize science and traditioanl culture for them. College students are learners of science, so we perform shadow puppetry in the school sports ground to popularize synthetic biology while spreading traditional culture for them. </td>
</tr>
<tr>
<td>Chinese paper cutting</td>
<td>Members of Traditional Culture Club</td>
- <td>The Traditional Culture Club in our school specializes in traditional culture. The paper-cutting form not only conforms to the nature of their club, but also allows them to learn about biological bacteria via this figurative way.</td>
+ <td>The Traditional Culture Club in our school specializes in traditional culture. The paper-cutting form not only conforms to the nature of their club, but also allows them to learn about biological bacteria via a figurative way.</td>
<tr>
<td> Monopoly Game</td>
<td>Children & Teenagers</td>
- <td>The history of synthetic biology will be popularized through the game methods that children and teenagers like.</td>
+ <td>The history of synthetic biology will be popularized through the game methods that children and teenagers prefer.</td>
</tr>
<tr>
<td>DNA model</td>
- <td>Childeren & Elderly without biological background</td>
+ <td>Children & Elderly without biological background</td>
<td>To explain what is DNA abstractly will only make people lose interest in synthetic biology. By visualizing DNA through DNA models, people can learn and play at the same time. </td>
</tr>
<tr>
@@ -79,18 +79,18 @@
</tr>
<tr>
<td>Breathing masks</td>
- <td>Science poplarized audiences & Interviewed enterprises</td>
- <td>We gave it to people that participated in the activities as a gift. </td>
+ <td>Science poplarized aundiences & Interviewed enterprises</td>
+ <td>We gave it to people who participated in the activities as a gift. </td>
</tr>
<tr>
<td>Social media</td>
<td>Public</td>
- <td>Social media can be able the public to learn about synthetic biology online in the situation of epidemic.</td>
+ <td>Social media can enable the public to learn about synthetic biology online in the situation of epidemic.</td>
</tr>
</table>
</div>
<h2 class='animate wiki-title2' data-animate='zoomIn'>Introduction</h2>
- <p>We believe that in addition to education, communication is also an important way to take synthetic biology anywhere. We used the materials which we've created to bring our project and synthetic biology to more people. </p>
+ <p>In addition to Education, Communication is also an important way to spread synthetic biology. Through self-made materials, we hope that more people will know about our project and synthetic biology. </p>
<h2 class='animate wiki-title2' data-animate='zoomIn'>Chinese shadow puppetry</h2>
<h3 class='animate wiki-title3'>Community</h3>
<h3 class='display-3'>Materials</h3>
@@ -98,7 +98,8 @@
<li>Chinese shadow puppetry.</li>
</ul>
<h3 class='display-3'>Targets</h3>
- <p>Children and the old who were in Liyayuan Community in Shenzhen.</p>
+ <p>Children and elderly who live in Liyayuan Community in Shenzhen.
+ </p>
<h3 class='display-3'>Process</h3>
<figure class='figure imgbox center-block'>
<img src='https://static.igem.wiki/teams/4325/wiki/page/communication/1/com-1-1.jpg' class='figure-img img-fluid rounded' alt='...'>
diff --git a/wiki/pages/contribution.html b/wiki/pages/contribution.html
index b954a0f9b909b5596d5fdea8cca5fa474201ce59..0d249c8a529b043f0a24c73da3094b8ebe737926 100644
--- a/wiki/pages/contribution.html
+++ b/wiki/pages/contribution.html
@@ -25,7 +25,7 @@
<img src='https://static.igem.wiki/teams/4325/wiki/page/contribution/figure-1.png' class='figure-img img-fluid rounded' alt='...'>
<figcaption class='figure-caption text-center'>Figure 1: Colony growth of <i>E. coli</i> TOP10 containing pDawn-RBSNNN-X174E-rrnB T1 (a) in the dark and (b) under blue light.</figcaption>
</figure>
- <p>We performed a new round of phenotyping assay for pDawn-RBSNNN-X174E-rrnB T1 (<a title="BBa_K3740044" href="http://parts.igem.org/Part:BBa_K3740044" target="_blank" rel="noopener">BBa_K3740044</a>). First, the monoclonal transformants of <em>E. coli </em>TOP10 with pDawn-RBSNNN-X174E-rrnB T1 was picked and grown on two parallel new LB plates, with one cultured in the dark and the other under blue light respectively to screen for correct builds. As shown in Figure 1, pSEVA331-pDawn-RBSNNN-X174E-rrnB T1 had a total of 10 strains which survived in the dark but failed to grow under blue light. This suggests that the blue light induced lysis system can function as intended in <em>E. coli</em> Top10.</p>
+ <p>We performed a new round of phenotyping assay for pDawn-RBSNNN-X174E-rrnB T1 (<a title="BBa_K3740044" href="http://parts.igem.org/Part:BBa_K3740044" target="_blank" rel="noopener">BBa_K3740044</a>). First, the monoclonal transformants of <em>E. coli </em>TOP10 with pDawn-RBSNNN-X174E-rrnB T1 were picked and grown on two parallel new LB plates, with one cultured in the dark and the other under blue light respectively to screen for correct builds. As shown in Figure 1, pSEVA331-pDawn-RBSNNN-X174E-rrnB T1 had a total of 10 strains which survived in the dark but failed to grow under blue light. This suggests that the blue light induced lysis system can function as intended in <em>E. coli</em> Top10.</p>
<p>From the above successful recombinant candidates (red squares in Figure 1), two bacterial strains were picked and grown in two parallel tubes of LB medium respectively, with one tube in the dark and the other tube under blue light to track. The OD<sub>600</sub><sub> </sub>values of the two strains cultured under the two different illumination conditions were measured at hourly intervals. As shown in Figure 2, both strains grew normally in the dark. For the #1 strain, the OD<sub>600</sub> value increased during the first four hours of blue light illumination and then declined significantly. This delay in lysis indicates it takes time for the lysis protein X174E to accumulate to lethal concentration under blue light. That is to say, the blue light sensitive lysis system of this strain is not efficient enough. For the #11 strain, the OD<sub>600</sub> value remained low during the first five hours of blue light illumination indicating the effectiveness of the system, but then increased dramatically implying that loss-of-function mutations could occurred in these genes or in the host genome and that the favorable mutant overtakes the parental population. Therefore, the blue light sensitive lysis system of this strain is not evolutionarily stable.</p>
<figure class='figure imgbox center-block'>
<img src='https://static.igem.wiki/teams/4325/wiki/page/contribution/figure-2.png' class='figure-img img-fluid rounded' alt='...'>
@@ -37,7 +37,7 @@
<img src='https://static.igem.wiki/teams/4325/wiki/page/contribution/figure-3.png' class='figure-img img-fluid rounded' alt='...'>
<figcaption class='figure-caption text-center'>Figure 3: Glutathione (GSH) content in the cell lysate of <em>Gluconacetobacter hansenii</em> ATCC53582 (<em>G. hansenii</em> ATCC53582) containing the plasmid of pSEVA331-J23102-RBS003422-gshF-T0 (CmR) and<em> G. hansenii</em> containing the plasmid of pSEVA331-J23102-RBS003422-gshF-T0 (AmpR).</figcaption>
</figure>
- <p>To enhance glutathione production in <em>G. hansenii</em> ATCC53582 , we constructed two expression systems of the bifunctional glutathione synthetase GshF on the basis of two pSEVA331 plasmid derivates, with one harboring the CmR gene while the other one carrying the AmpR gene. The resultant two plasmids of pSEVA331-gshF were introduced into <em>G. hansenii</em> by electroporation separately. As shown in Figure 3, the two recombinant strains with different antibiotic resistance have significantly different levels of glutathione. One possible reason is that resistance gene influences the expression of GshF. It is also likely that the antibiotic molecules It is also likely that the antibiotic molecules affect the activity of GshF. We hope. that future iGEMers will make different attempts on the resistance genes in their vectors to optimize their system. </p>
+ <p>To enhance glutathione production in <em>G. hansenii</em> ATCC53582 , we constructed two expression systems of the bifunctional glutathione synthetase GshF on the basis of two pSEVA331 plasmid derivates, with one harboring the CmR gene while the other one carrying the AmpR gene. The resultant two plasmids of pSEVA331-gshF were introduced into <em>G. hansenii</em> by electroporation separately. As shown in Figure 3, the two recombinant strains with different antibiotic resistance have significantly different levels of glutathione. One possible reason is that resistance gene influences the expression of GshF. It is also likely that the antibiotic molecules affect the activity of GshF. We hope that future iGEMers will make different attempts on the resistance genes in their vectors to optimize their system. </p>
<h2 class='animate wiki-title2' data-animate='zoomIn'>Antibiotic concentration in medium could have an influence on bacterial growth</h2>
<figure class='figure imgbox center-block'>
<img src='https://static.igem.wiki/teams/4325/wiki/page/contribution/figure-4.png' class='figure-img img-fluid rounded' alt='...'>
diff --git a/wiki/pages/hp-dialogue-2.html b/wiki/pages/hp-dialogue-2.html
index 8accabb978d44f1b0fb7ca5079c140050910993b..4058de6e5376c67a9d6bebe7461671b4d20f12a6 100644
--- a/wiki/pages/hp-dialogue-2.html
+++ b/wiki/pages/hp-dialogue-2.html
@@ -144,7 +144,7 @@
<span style="text-align: center">Dr. Penggao Dai</span>
</div>
<div class="hp-diacontent">
- <div class="tip right"><p>If your project will fully developed into a real product that real people could use, I recommend that you choose a squeeze mask (activating liquid and freeze-dried powder) as your product form, which can improve bacterial activity. And the shelf life of the squeeze mask will be longer than the shelf life of the bacterial emulsion.</p></div>
+ <div class="tip right"><p>In addition to the live bacterial emulsion, I think that freeze-dried powder is also a good product format considering its longer shelf life.</p></div>
</div>
</div>
diff --git a/wiki/pages/human-practices.html b/wiki/pages/human-practices.html
index bc9908534b25e9da0249a0d9425d6bdb341c892a..36abb9bce1683a70a9ced2bb64f5f92434a5d306 100644
--- a/wiki/pages/human-practices.html
+++ b/wiki/pages/human-practices.html
@@ -106,16 +106,13 @@
After certain research, <strong>we found that glutathione(GSH) small molecules can be absorbed by the skin</strong>, which again proves the feasibility of our project product. Also, considering the safety when users use our product on nonintact skin raised by the dermatologist, we decided to further improve the product. Later, we will indicate on our product package that "It is not recommended to use the product on nonintact skin". In addition, <strong>in order to avoid others</strong><strong>' </strong><strong>feeling that our project is too abstract, we have made different versions of brochures to better promote our project</strong>. <strong>(See "<a href="{{ url_for('pages', page='communication') }}" target='_blank'>Communication</a>" for more details)</strong>
</p>
<h2 class='animate wiki-title2' data-animate='zoomIn'>Experimental Design</h2>
- <h3 class='animate wiki-title3'>The GshF expression and GSH production module</h3>
+ <h3 class='animate wiki-title3'>The lysis and safety module</h3>
<h3 class='display-3'>Interview with Dr. Mingxing Tang</h3>
<figure class='figure imgbox center-block'>
<img src='https://static.igem.wiki/teams/4325/wiki/page/ihp/experiment-design/ihp1.jpg' class='figure-img img-fluid rounded' alt='...'>
<figcaption class='figure-caption text-center'>Figure 12: Interviewed with Dr. Mingxing Tang on June 16<sup>th</sup></figcaption>
</figure>
- <p>Our team completed the preliminary design of the experiment under the guidance of the PI. We contacted Dr. Mingxing Tang (Figure 12). We introduced our project, and he was very optimistic about the preliminary design of our experiments. He suggested codon optimization could increase the production of glutathione.</p>
- <h3 class='animate wiki-title3'>The Lysis and Safety module</h3>
- <h3 class='display-3'>Interview with Dr. Mingxing Tang</h3>
- <p>Based on the research foundation of last year's project, we optimized the lysis and safety module of the project to minimize the pollution to the environment. Our team paid a return visit to Dr. Mingxing Tang. At the beginning of the experiment of lysing <em>G. hansenii </em>under blue light, we found that the constructed blue light responsive system is unstable. He suggested removing the LVA tage from pDawn (cl-LVA) to reduce the background expression of the lysis gene and improve the stability of the blue light responsive system. Based on the above suggestions, we found that strains containing the modified plasmids grown to high concentrations with blue light on were able to lyse through a series of experiments. (See "<a href="{{ url_for('pages', page='improve') }}" target='_blank'>improvement</a>" for more details)</p>
+ <p>Based on our project from last year, we tried to optimize the lysis and safety modules. When we repeated the lysis experiment, we found that the constructed blue light reaction system did not work well in rapidly growing cells. Therefore, we contacted Dr. Mingxing Tang (Figure 12). He suggested removing the LVA tag from the repressor cI in pDawn to reduce the leaky expression of lysis genes, which might contribute to the stability of the blue light response system. (See "<a href="{{ url_for('pages', page='improve') }}" target='_blank'>improvement</a>" for more details)</p>
<h2 class='animate wiki-title2' data-animate='zoomIn'>Product Form</h2>
<figure class='figure imgbox center-block'>
<img src='https://static.igem.wiki/teams/4325/wiki/page/ihp/product-form/ihp13.png' class='figure-img img-fluid rounded' alt='...' style="width: 65%;max-height: none;">
@@ -143,7 +140,7 @@
<p>During the communication and cooperation with the NWU-China-A, we helped each other to find the company we needed ( Dr. Penggao Dai, Technical Director Shaanxi Lifegne Co., Ltd.). Due to the severe of epidemic, we interviewed Dr. Penggao Dai of Shaanxi Lifegne Co., Ltd. online (Figure 15, 16). He provided us with useful information from the perspective of working in the field for almost 20 years. Here's the conversation we had:</p>
{% include 'pages/hp-dialogue-2.html' %}
<h3 class='display-3'>Conclusion</h3>
- <p>After brainstorming, we decided on two product forms: bacterial emulsion and squeeze mask (activating liquid and freeze-dried powder)</p>
+ <p>Considering that the bacterial emulsion is easier to use, we finally decided not to use freeze-dried powder as our product form.</p>
<h3 class='animate wiki-title3'>Interview with Bode Chuangyan (Guangzhou) Biotechnology Co., Ltd.</h3>
<figure class='figure imgbox center-block'>
<img src='https://static.igem.wiki/teams/4325/wiki/page/ihp/product-form/ihp17.png' class='figure-img img-fluid rounded' alt='...'>
@@ -155,7 +152,7 @@
</figure>
<p>Is there any better methods can we use to evaluate the efficacy of the product? How to adjust the bacterial emulsion formulations? Therefore, we interviewed and consulted the technician of Bode Chuangyan company offline(Figure 17, 18). Our team visited Bode's lab and presented our project to the technician of Bode Chuangyan. She suggested that we may improve the toughness of the bacterial cellulose film by adjusting the ratio of the thickening agent.</p>
<h3 class='display-3'>Conclusion</h3>
- <p>The product form originally envisaged was the bacterial emulsion, but after many formulation and performance experiments, our product is presented in the form of live bacterial emulsion/gel. This is more conducive to later use and product efficacy (See "<a href="{{ url_for('pages', page='proof-of-concept') }}" target='_blank'>Proof of Concept</a>" for more details). In addition, we learned that zebrafish eggs can be used for antioxidant efficacy assays. However, considering of biodiversity, we finally decided to abandon the method of using zebrafish eggs for the detection of antioxidant efficacy, but to use the free radical kit (See "<a href="{{ url_for('pages', page='experiments') }}#GSHconcentrationtestmodule" target='_blank'>Experiments</a>" for more details). This interview pushed our project forward.</p>
+ <p>The product form originally envisaged was the bacterial emulsion, but after many formulation and performance experiments, our product is presented in the form of live bacterial emulsion/gel. This is more conducive to later use and product efficacy. (See "<a href="{{ url_for('pages', page='proof-of-concept') }}" target='_blank'>Proof of Concept</a>" for more details) In addition, we learned that zebrafish eggs can be used for antioxidant efficacy assays. However, considering the welfare of animals, we finally decided to abandon the method of using zebrafish eggs for the detection of antioxidant efficacy, but used Glutathione Colorimetric Detection Kit (Beyotime). (See "<a href="{{ url_for('pages', page='experiments') }}#GSHconcentrationtestmodule" target='_blank'>Experiments</a>" for more details). This interview pushed our project forward.</p>
<h3 class='animate wiki-title3'>Equipment Design</h3>
<figure class='figure imgbox center-block'>
<img src='https://static.igem.wiki/teams/4325/wiki/page/ihp/product-form/ihp19.png' class='figure-img img-fluid rounded' alt='...'>
diff --git a/wiki/pages/improve.html b/wiki/pages/improve.html
index b3ac9ba2150b3951e45b6c1010e8e5d21e428acb..6bbb57e5588bc873b2c03da39a97982408c3031b 100644
--- a/wiki/pages/improve.html
+++ b/wiki/pages/improve.html
@@ -21,7 +21,7 @@
<img src='https://static.igem.wiki/teams/4325/wiki/page/design/project4.jpeg' class='figure-img img-fluid rounded' alt='...'>
<figcaption class='figure-caption text-center'>Figure 1: The genetic circuit of the lysis and safety module</figcaption>
</figure>
- <p>The blue light response part pDawn is used to control the expression of lysis genes and characterized in the resultant system (Figure 1). The host chassis expressing this system is expected to grow normally without blue light and to lyse under blue light. However, we found that a significant proportion of the recombinant colonies did not grow very well in the dark, indicating a leak expression of the lysis genes (Figure 2-a). The colony which grew normally in the dark was picked and grown to logarithmic growth phase. But the strain continued growing under blue light, which means that the system became disabled in rapidly growing cells (Figure 2-b). It is suggestd that the abovementioned leaky expression of the lysis genes leads to loss-of function mutation and that the favorable mutant overtakes the parental population.</p>
+ <p>The blue light response part pDawn is used to control the expression of lysis genes and characterized in the resultant system (Figure 1). The host chassis expressing this system is expected to grow normally without blue light and to lyse under blue light. However, we found that a significant proportion of the recombinant colonies did not grow very well in the dark, indicating a leak expression of the lysis genes (Figure 2-a). The colony which grew normally in the dark was picked and grown to logarithmic growth phase. But the strain continued growing under blue light, which means that the system became disabled in rapidly growing cells (Figure 2-b). It is suggestd that the above mentioned leaky expression of the lysis genes leads to loss-of function mutation and that the favorable mutant overtakes the parental population.</p>
<figure class='figure imgbox center-block'>
<img src='https://static.igem.wiki/teams/4325/wiki/page/improvement/improvement2.jpg' class='figure-img img-fluid rounded' alt='...'>
<figcaption class='figure-caption text-center'>Figure 2: (a) Colony growth of <i>E. coli</i> TOP10 containing pDawn(cI-LVA)-RBS070-LKD in the dark<br>(b) Growth curve of <i>E. coli</i> TOP10 containing pDawn(cI-LVA)-RBS070-LKD</figcaption>
diff --git a/wiki/pages/timeline.html b/wiki/pages/timeline.html
index b3a4ac63db398b8a02ebc39745e1d3c38fc9828f..d37d89f67a7b94d03d9d2d929831fd09a1e59cc0 100644
--- a/wiki/pages/timeline.html
+++ b/wiki/pages/timeline.html
@@ -418,8 +418,8 @@
<img src="https://static.igem.wiki/teams/4325/wiki/page/ihp/timeline/ihp14.png" class="rounded-circle shadow imgbox-img" >
</div>
<ul>
- <li>They suggested that we can consider squeeze mask in the form of our product with activating liquid and freeze-dried powder, which can increase bacterial activity.</li>
- <li>We took the suggestion and decided to develop two forms of the product: 1. emulsion, 2. Squeeze mask (activating liquid and freeze-dried powder)</li>
+ <li>After we expressed our preliminary idea of using a live bacterial emulsion as the product form, he proposed that freeze-dried powder is also a good product format considering its longer shelf life.</li>
+ <li>Considering that the bacterial emulsion is easier to use, we finally decided not to use freeze-dried powder as our product form.</li>
</ul>
</div>
</div>
@@ -441,7 +441,7 @@
<li>We learned that zebrafish eggs can be used for antioxidant efficacy testing.</li>
<li style="list-style: circle;">The product form originally envisaged by our project was an emulsion, but after several preparations and performance experiments, we have improved the emulsion product to a gel product. It also facilitated the progress of our project.</li>
- <li>Considering of biodiversity, we finally decided to abandon the method of using zebrafish eggs for the detection of antioxidant efficacy, and used a free radical kit to assay glutathione content.</li>
+ <li>Considering the welfare of animals, we finally decided to abandon the method of using zebrafish eggs for the detection of antioxidant efficacy, but used Glutathione Colorimetric Detection Kit (Beyotime).</li>
</ul>
</div>
</div>