From 41afb555769974d0e301d36d5fcb2245340f426d Mon Sep 17 00:00:00 2001
From: fangfang2333 <3101867908@qq.com>
Date: Thu, 13 Oct 2022 21:56:04 +0800
Subject: [PATCH] 5556655
---
wiki/pages/attributions.html | 16 ++++++++--------
wiki/pages/collaborations.html | 6 ++----
wiki/pages/human-practices.html | 2 +-
wiki/pages/improve.html | 4 ++--
wiki/pages/timeline.html | 2 +-
5 files changed, 14 insertions(+), 16 deletions(-)
diff --git a/wiki/pages/attributions.html b/wiki/pages/attributions.html
index 447b4fa..2103778 100644
--- a/wiki/pages/attributions.html
+++ b/wiki/pages/attributions.html
@@ -78,7 +78,7 @@
<div class="col-lg-9">
<div id="postContentDiv" class="span9">
<h2 class="animate wiki-title2" data-animate="zoomIn" >Overview</h2>
- <p>SZPT-CHINA is a team composed of 15 young students who are passionate about synthetic biology. <strong>The goal of our project</strong> is to use <em>Beauty G. hansenii</em> to produce a live bacterial skin care product with various efficacy, based on the project of SZPT-CHINA in <a href='https://2021.igem.org/Team:SZPT-CHINA' target='_blank'>"2021 self-growth artificial skin for burn treatment"</a>. After months of research, experiments and collaboration, SZPT-CHINA has developed a live bacterial skin care prototype with anti-oxidant and skin microbiome maintenance efficacy. <strong>This not only uncovers a new possibility for skincare products</strong><strong>, </strong><strong>but also provides a brand-new method of preserving active ingredients.</strong> We would like to express our sincere gratitude to all the team members, as well as to the other iGEM team members and experts who have helped us tremendously in this process.</p>
+ <p>SZPT-CHINA is a team composed of 15 young students who are passionate about synthetic biology. <strong>The goal of our project</strong> is to use <em>Beauty G. hansenii</em> to produce a live bacterial skin care product with various efficacy, based on the project of SZPT-CHINA in <a href='https://2021.igem.org/Team:SZPT-CHINA' target='_blank'>"2021 self-growth artificial skin for burn treatment"</a>. After months of researchs, experiments and collaborations, SZPT-CHINA has developed a live bacterial skin care prototype with anti-oxidant and skin microbiome maintenance efficacy. <strong>This not only uncovers a new possibility for skincare products</strong><strong>, </strong><strong>but also provides a brand-new method of preserving active ingredients.</strong> We would like to express our sincere gratitude to all the team members, as well as to the other iGEM team members and experts who have helped us tremendously in this process.</p>
<h2 class='animate wiki-title2' data-animate='zoomIn'>Team members</h2>
<h3 class='animate wiki-title3'>In a table</h3>
@@ -99,13 +99,13 @@
<div class="teacher-say-box row">
<div class="tea-say-content col-md-12 col-xs-12">
- <p> <b>Jiayi Chen: </b>Leader of the HP group, responsible for social outreach and practice. She is also in charge of the beautification of the PowerPoint resentation and writing the wiki pages of integrated hunman practices. </p>
+ <p> <b>Jiayi Chen: </b>Leader of the HP group, responsible for social outreach and practice. She is also in charge of the beautification of the PowerPoint presentation and writing the wiki page of integrated human practices. </p>
</div>
</div>
<div class="teacher-say-box row">
<div class="tea-say-content col-md-12 col-xs-12">
- <p> <b>Jiali Chen: </b>Leader of the art group, responsible for science education through social media. She is also participated in some Art work, such as the design of wiki pages and the project's brochures and posters, etc. In addition, she responsible for writing the wiki pages of members and attributions.</p>
+ <p> <b>Jiali Chen: </b>Leader of the ART group, responsible for science education through social media. She is also participated in some Art work, such as the design of wiki pages and the project's brochures and posters, etc. In addition, she responsible for writing the wiki pages of members and attributions.</p>
</div>
</div>
@@ -127,12 +127,12 @@
</div>
<div class="teacher-say-box row">
<div class="tea-say-content col-md-12 col-xs-12">
- <p> <b>Yiyuan Zhang: </b>Team member in art group, responsible for art design of wiki and project illustration. He is also actively involved in some education activities.</p>
+ <p> <b>Yiyuan Zhang: </b>Team member in ART group, responsible for art design of wiki and project illustration. He is also actively involved in some education activities.</p>
</div>
</div>
<div class="teacher-say-box row">
<div class="tea-say-content col-md-12 col-xs-12">
- <p> <b>Zijian Zhu: </b>Team member in HP group, one of presenter of our team, responsible for project presentation and English proofreading. He also contributed to writing the wiki pages of description.</p>
+ <p> <b>Zijian Zhu: </b>Team member in HP group, one of presenters of our team, responsible for project presentation and English proofreading. He also contributed to writing the wiki pages of description.</p>
</div>
</div>
<div class="teacher-say-box row">
@@ -154,13 +154,13 @@
<div class="teacher-say-box row">
<div class="tea-say-content col-md-12 col-xs-12">
- <p> <b>Minchun He: </b>Team member of EXP group, responsible for bacterial fluid and GSH production. She is also contributed to writing the wiki pages of results.</p>
+ <p> <b>Minchun He: </b>Team member of EXP group, responsible for bacterial fluid and GSH production. She is also contributed to writing the wiki page of results.</p>
</div>
</div>
<div class="teacher-say-box row">
<div class="tea-say-content col-md-12 col-xs-12">
- <p> <b>Laiyi Teng: </b>Team member of EXP group, responsible for tracking experiment data and providing experiment materials. She is also in charge of writing the wiki pages of contribution.</p>
+ <p> <b>Laiyi Teng: </b>Team member of EXP group, responsible for tracking experiment data and providing experiment materials. She is also in charge of writing the wiki page of contribution.</p>
</div>
</div>
@@ -172,7 +172,7 @@
<div class="teacher-say-box row">
<div class="tea-say-content col-md-12 col-xs-12">
- <p> <b>Chujuan Cai: </b>Team member of EXP group, contributed to exploring the influence of different factors on <em>G. hansenni</em> emulsion. She is also responsible for writing the wiki pages of Engineering Success.</p>
+ <p> <b>Chujuan Cai: </b>Team member of EXP group, contributed to exploring the influence of different factors on <em>G. hansenni</em> emulsion. She is also responsible for writing the wiki page of Engineering Success.</p>
</div>
</div>
diff --git a/wiki/pages/collaborations.html b/wiki/pages/collaborations.html
index 34e44de..c070dc7 100644
--- a/wiki/pages/collaborations.html
+++ b/wiki/pages/collaborations.html
@@ -27,16 +27,14 @@
<img src="https://static.igem.wiki/teams/4325/wiki/page/collaboration/collaboration1.jpeg" class="figure-img img-fluid rounded" alt="...">
<figcaption class="figure-caption text-center">Figure 1: We had our online meeting with NWU-CHINA-A on July 19<sup>th</sup></figcaption>
</figure>
- <p>Last year, our team has cooperated with NWU-CHINA-A. Based on our common interests and friendly cooperation, we continue to cooperate with NWU-CHINA-A this year. So far, we have <b>conducted three joint presentations</b> with their team, namely the 2022 China iGEM Online Meetup on July 16<sup>th</sup>, the 4th Northwestern China iGEM Meetup hosted by them on July 23<sup>rd</sup>, and the one we hosted on August 6<sup>th</sup> with nearly 300 participants. In addition, we also carried out extensive and in-depth exchanges on HP and experiments. In terms of HP, NWU-CHINA-A recommended us an expert in pharmacogenomics, namely Professor Penggao Dai of Northwest University, and introduced us the Shaanxi Lifegen CO. ,Ltd., On August 10<sup>th</sup> this year, we conducted an online interview with this company and learned a lot in the process. In terms of experiments, we hold regular exchange meetings every week to discuss and supervise each other, and jointly <b>solved the difficulties </b>and <b>bottlenecks encountered in our project</b>.</p>
+ <p>Last year, our team has cooperated with NWU-CHINA-A. Based on our common interests and friendly cooperation, we continue to cooperate with NWU-CHINA-A this year. So far, we have <b>conducted three joint presentations</b> with their team, namely the 2022 China iGEM Online Meetup on July 16<sup>th</sup>, the 4th Northwestern China iGEM Meetup hosted by them on July 23<sup>rd</sup>, and the one we hosted on August 6<sup>th</sup> with nearly 300 participants. We also carried out extensive and in-depth collaborations on HP and experiments. In terms of HP, NWU-CHINA-A recommended us an expert in pharmacogenomics, namely Professor Penggao Dai of Northwest University, and introduced us the Shaanxi Lifegen CO. ,Ltd., On August 10<sup>th</sup> this year, we conducted an online interview with this company and learned a lot in the process. In terms of experiments, we hold regular exchange meetings every week to discuss and supervise each other, and jointly <b>solved the difficulties </b>and <b>bottlenecks encountered in our project</b>.</p>
<h2 class="animate wiki-title2" data-animate="zoomIn" >BNUZH-China</h2>
<figure class="figure imgbox center-block">
<img src="https://static.igem.wiki/teams/4325/wiki/page/collaboration/collaboration2.png" class="figure-img img-fluid rounded" alt="...">
<figcaption class="figure-caption text-center">Figure 2: We conducted a joint presentation of five schools on August 6<sup>th</sup></figcaption>
</figure>
- <p>When communicating with BNUZH-China, we shared our projects' progress with each other and solved problems together. For example, when they could not contact the experts, we suggested them to go to the local hospital to register and interview the experts as patients. All these suggestions are based on our own practice and experience last year and have helped their team solve many practical difficulties. On June 3<sup>rd</sup> this year, we participated in the Southern China Regional Meeting with BNUZH-China. On August 6<sup>th</sup>, we also invited BNUZH-China to participate in the online joint presentation of "Into Synthetic Biology" hosted by us to jointly introduce related projects.</p>
-
-
+ <p>When communicating with BNUZH-China, we shared our projects' progress with each other and solved problems together. For example, when they could not contact the experts, we suggested them to go to the local hospital to register and interview the experts as patients. All the suggestions are based on our own practice and experience last year and have helped them solve many practical difficulties. On June 3<sup>rd</sup> this year, we participated in the Southern China Regional Meeting with BNUZH-China. On August 6<sup>th</sup>, we also invited BNUZH-China to participate in an online joint presentation -- "Into Synthetic Biology" hosted by us to jointly introduce related projects.</p>
<h2 class="animate wiki-title2" data-animate="zoomIn" >HBUT-China</h2>
<figure class="figure imgbox center-block">
<img src="https://static.igem.wiki/teams/4325/wiki/page/collaboration/collaboration3.png" class="figure-img img-fluid rounded" alt="...">
diff --git a/wiki/pages/human-practices.html b/wiki/pages/human-practices.html
index 36abb9b..3b453f3 100644
--- a/wiki/pages/human-practices.html
+++ b/wiki/pages/human-practices.html
@@ -152,7 +152,7 @@
</figure>
<p>Is there any better methods can we use to evaluate the efficacy of the product? How to adjust the bacterial emulsion formulations? Therefore, we interviewed and consulted the technician of Bode Chuangyan company offline(Figure 17, 18). Our team visited Bode's lab and presented our project to the technician of Bode Chuangyan. She suggested that we may improve the toughness of the bacterial cellulose film by adjusting the ratio of the thickening agent.</p>
<h3 class='display-3'>Conclusion</h3>
- <p>The product form originally envisaged was the bacterial emulsion, but after many formulation and performance experiments, our product is presented in the form of live bacterial emulsion/gel. This is more conducive to later use and product efficacy. (See "<a href="{{ url_for('pages', page='proof-of-concept') }}" target='_blank'>Proof of Concept</a>" for more details) In addition, we learned that zebrafish eggs can be used for antioxidant efficacy assays. However, considering the welfare of animals, we finally decided to abandon the method of using zebrafish eggs for the detection of antioxidant efficacy, but used Glutathione Colorimetric Detection Kit (Beyotime). (See "<a href="{{ url_for('pages', page='experiments') }}#GSHconcentrationtestmodule" target='_blank'>Experiments</a>" for more details). This interview pushed our project forward.</p>
+ <p>The product form originally envisaged was the bacterial emulsion, but after many formulation and performance experiments, our product is presented in the form of live bacterial emulsion/gel. This is more conducive to later use and product efficacy. (See "<a href="{{ url_for('pages', page='proof-of-concept') }}" target='_blank'>Proof of Concept</a>" for more details) In addition, we learned that zebrafish eggs can be used for antioxidant efficacy assays. However, Considering the welfare of animals, we finally decided to abandon the method of using zebrafish eggs for the detection of antioxidant efficacy, but used a free radical kit (in vitro). (See "<a href="{{ url_for('pages', page='experiments') }}#GSHconcentrationtestmodule" target='_blank'>Experiments</a>" for more details) This interview pushed our project forward.</p>
<h3 class='animate wiki-title3'>Equipment Design</h3>
<figure class='figure imgbox center-block'>
<img src='https://static.igem.wiki/teams/4325/wiki/page/ihp/product-form/ihp19.png' class='figure-img img-fluid rounded' alt='...'>
diff --git a/wiki/pages/improve.html b/wiki/pages/improve.html
index 6bbb57e..792f785 100644
--- a/wiki/pages/improve.html
+++ b/wiki/pages/improve.html
@@ -21,7 +21,7 @@
<img src='https://static.igem.wiki/teams/4325/wiki/page/design/project4.jpeg' class='figure-img img-fluid rounded' alt='...'>
<figcaption class='figure-caption text-center'>Figure 1: The genetic circuit of the lysis and safety module</figcaption>
</figure>
- <p>The blue light response part pDawn is used to control the expression of lysis genes and characterized in the resultant system (Figure 1). The host chassis expressing this system is expected to grow normally without blue light and to lyse under blue light. However, we found that a significant proportion of the recombinant colonies did not grow very well in the dark, indicating a leak expression of the lysis genes (Figure 2-a). The colony which grew normally in the dark was picked and grown to logarithmic growth phase. But the strain continued growing under blue light, which means that the system became disabled in rapidly growing cells (Figure 2-b). It is suggestd that the above mentioned leaky expression of the lysis genes leads to loss-of function mutation and that the favorable mutant overtakes the parental population.</p>
+ <p>The blue light response part pDawn is used to control the expression of lysis genes and characterized in the resultant system (Figure 1). The host chassis expressing this system is expected to grow normally without blue light and to lyse under blue light. However, we found that a significant proportion of the recombinant colonies did not grow very well in the dark, indicating a leak expression of the lysis genes (Figure 2-a). The colony which grew normally in the dark was picked and grown to logarithmic growth phase. But the strain continued growing under blue light, which means that the system became disabled in rapidly growing cells (Figure 2-b). It is suggestd that the above mentioned leaky expression of the lysis genes leads to loss-of-function mutation and that the favorable mutant overtakes the parental population.</p>
<figure class='figure imgbox center-block'>
<img src='https://static.igem.wiki/teams/4325/wiki/page/improvement/improvement2.jpg' class='figure-img img-fluid rounded' alt='...'>
<figcaption class='figure-caption text-center'>Figure 2: (a) Colony growth of <i>E. coli</i> TOP10 containing pDawn(cI-LVA)-RBS070-LKD in the dark<br>(b) Growth curve of <i>E. coli</i> TOP10 containing pDawn(cI-LVA)-RBS070-LKD</figcaption>
@@ -50,7 +50,7 @@
<figcaption class='figure-caption text-center'>Figure 5:
Colony growth of <i>E. coli</i> TOP10 containing the plasmid of (a) pDawn(cI-LVA)-RBS070-LKD in the dark and (b) pDawn(cI)-RBS070-LKD in the dark</figcaption>
</figure>
- <p>From the above two groups of successful recombinant candidates (red squares in Figure 1), one colony was picked and grow in LB media in the dark until ~OD<sub>600</sub> 0.6 was reached to track the growth condition under blue light. Then the OD<sub>600</sub> values of the two selected strains under blue light was measured to compare the effectiveness of the two systems in rapidly growing cells. We found that both of the strain continued to grow during the first few hours of blue light illumination. However, only the strain containing the plasmid of pDawn(cI)-RBS070-LKD showed a significant decrease in OD<sub>600</sub> value, indicating maintenance of lethality. And the OD<sub>600</sub> value of the strain containing the plasmid of pDawn(cI)-RBS070-LKD increases a little bit, implying that loss-of-function mutations could occurred in these genes or in the host genome and that the favorable mutant overtakes the parental population. Therefore, the new pDawn in which the LVA tag of cI is deleted increases the evolutionary stability of the blue light sensitive kill switch by negating the deleterious evolutionary pressure of leaky toxin expression.</p>
+ <p>From the above two groups of successful recombinant candidates (red squares in Figure 1), one colony was picked and grown in LB media in the dark until ~OD<sub>600</sub> 0.6 was reached to track the growth condition under blue light. Then the OD<sub>600</sub> values of the two selected strains under blue light was measured to compare the effectiveness of the two systems in rapidly growing cells. We found that both of the strain continued to grow during the first few hours of blue light illumination. However, only the strain containing the plasmid of pDawn(cI)-RBS070-LKD showed a significant decrease in OD<sub>600</sub> value, indicating maintenance of lethality. And the OD<sub>600</sub> value of the strain containing the plasmid of pDawn(cI)-RBS070-LKD increases a little bit, implying that loss-of-function mutations could occurred in these genes or in the host genome and that the favorable mutant overtakes the parental population. Therefore, the new pDawn in which the LVA tag of cI is deleted increases the evolutionary stability of the blue light sensitive kill switch by negating the deleterious evolutionary pressure of leaky toxin expression.</p>
<figure class='figure imgbox center-block'>
<img src='https://static.igem.wiki/teams/4325/wiki/page/improvement/improvement6.jpg
diff --git a/wiki/pages/timeline.html b/wiki/pages/timeline.html
index d37d89f..5fe6ab7 100644
--- a/wiki/pages/timeline.html
+++ b/wiki/pages/timeline.html
@@ -441,7 +441,7 @@
<li>We learned that zebrafish eggs can be used for antioxidant efficacy testing.</li>
<li style="list-style: circle;">The product form originally envisaged by our project was an emulsion, but after several preparations and performance experiments, we have improved the emulsion product to a gel product. It also facilitated the progress of our project.</li>
- <li>Considering the welfare of animals, we finally decided to abandon the method of using zebrafish eggs for the detection of antioxidant efficacy, but used Glutathione Colorimetric Detection Kit (Beyotime).</li>
+ <li>Considering the welfare of animals, we finally decided to abandon the method of using zebrafish eggs for the detection of antioxidant efficacy, but used a free radical kit (in vitro).</li>
</ul>
</div>
</div>
--
GitLab