NEFU_China

Our target product is red, after we introduced the exogenous synthesis pathway into the brewer's yeast genome, the bacteria that produce the target product will turn red, we coated the plate medium and found that at first there were red single colonies, slowly white strains grew on the red single colonies, what is the reason? Is it degraded or is the yeast single colony impure (not really a single colony)?

Wenwen Xiao

Naked eye observation sometimes has bias and you can try to use microscope for further observation.

Shengwei fu

It is also recommended to delineate the red colonies several times and sequence the promoter and RBS of the white colonies to see if they are mutated.