LB (Lysogeny broth)is the most commonly used nutrient medium in microbiology experiments for culturing bacteria such as E. coli, and is divided into liquid medium and solid medium made by adding agar. LB medium with antibiotics added can be used to screen selected colonies with E. coli as the host.
The method for the preparation of 1 litre of LB:
Because 1 L of LB is too much for our experiment, we’ve divided each dosage by 4
Yeast extract peptone dextrose (YEPD) is a complete medium for yeast growth. It contains yeast extract, peptone, double-distilled water, and glucose (dextrose). It can be used as solid medium by including agar. The yeast extract will typically contain all the amino acids necessary for growth.
Yeast extract 10 g
Tryptone 20 g
Dexyrose 20 g
1000 mL of distilled water
We’ve also divide each dosage by 4:
Yeast extract 2.5 g
Tryptone 5 g
Dexyrose 5 g
250 mL of distilled water
medium into petri dish in the clean bench.