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{% block title %}Part Collection{% endblock %}
{% block lead %}We developed part collection for a Cyanophage toolkit to facilitate Cyanobacterial engineering.{% endblock %}
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Our team has put together a parts collection. This collection is intended for use in the construction of a replication-incompetent ghost Cyanophage. The phage is constructed from capsid components of S-TIP37, a Cyanophage with known host specificity for Synechococcus sp. WH8102 and WH8109, lab adapted marine strains of Cyanobacteria that have similarities to Synechococcus PCC11901, an emerging synthetic biology chassis.
This parts collection is intended for MoClo assembly and has been designed to be compatible with the iGEM Type IIS assembly via Golden Gate. Basic parts represent the level 0 assembly and are taken from S-TIP37 coding sequences published on The National Institutes of Health National Center for Biotechnology Information (NIH NCBI) Gene bank. Level 1 parts include a promoter, RBS, and terminator sequence to create transcriptional units. Level 2 parts are multi-transcriptional unit assemblies, and level 3 parts represent the assembly of the multi-transcriptional units into the final phagemid. All parts are published in the iGEM parts repository. The table below provides links to each part. An annotated Master List, which provides more details about the design of each part, can be accessed here.
Part | Part Type | Accession # | Description |
---|---|---|---|
BBa_K4268000 | Coding/Basic | NC_048026.1 | S-TIP37 Head-Tail Connector |
BBa_K4268001 | Coding/Basic | NC_048026.1 | S-TIP37 Capsid Assembly Protein |
BBa_K4268002 | Coding/Basic | NC_048026.1 | S-TIP37 Capsid Protein |
BBa_K4268003 | Coding/Basic | NC_048026.1 | S-TIP37 Capsid Protein with Biotin Tag |
BBa_K4268004 | Coding/Basic | NC_048026.1 | S-TIP37 Tail Tubular Protein A |
BBa_K4268005 | Coding/Basic | NC_048026.1 | S-TIP37 Tail Tubular Protein B |
BBa_K4268006 | Coding/Basic | NC_048026.1 | S-TIP37 Internal Core Protein |
BBa_K4268007 | Coding/Basic | NC_048026.1 | S-TIP37 Tail Fiber-Like Protein |
BBa_K4268008 | Composite | Level 1 Head-Tail Connector | |
BBa_K4268009 | Composite | Level 1 Capsid Assembly Protein | |
BBa_K42680010 | Composite | Level 1 Capsid Protein | |
BBa_K4268011 | Composite | Level 1 Capsid Protein with Biotin Tag | |
BBa_K4268012 | Composite | Level 1 Tail Tubular Protein A | |
BBa_K4268013 | Composite | Level 1 Tail Tubular Protein B | |
BBa_K4268014 | Composite | Level 1 Internal Core Protein | |
BBa_K4268015 | Composite | Level 1 Tail Fiber-Like Protein | |
BBa_K4268016 | Composite | Neck and Tail Multi-Transcriptional Unit | |
BBa_K4268017 | Composite | Untagged Capsid and Core Multi-Transcriptional Unit | |
BBa_K4268018 | Composite | Biotin Tagged Capsid and Core Multi-Transcriptional Unit | |
BBa_K4268019 | Composite | S-TIP 37 Ghost Phagemid | |
BBa_K4268020 | Composite | S-TIP 37 Ghost Phagemid with Biotin-tagged Capsid |