diff --git a/wiki/pages/experiments.html b/wiki/pages/experiments.html
index c10ce78d045a45a1ff8ed4dfc97abc0dbfc36daf..d4d1fb19a007c83070675f425b2a463022c40604 100644
--- a/wiki/pages/experiments.html
+++ b/wiki/pages/experiments.html
@@ -4,26 +4,28 @@
{% block page_content %}
-<h1>Cloning and Expression System </h1>
+<h1 class="reveal-on-scroll">Cloning and Expression System </h1>
-<p>For our experimentation, expression of MAM7 was done by using our chassis– <i>E.coli</i> BL21 and the vector
+<p class="reveal-on-scroll">For our experimentation, expression of MAM7 was done by using our chassis– <i>E.coli</i>
+ BL21 and the vector
pET-22b(+).</p>
-<figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/mam7-cloning-plasmid.png"
+<figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/mam7-cloning-plasmid.png"
class="figure-img img-fluid rounded" alt="MAM7 cloning" width="50%">
- <figcaption class="figure-caption text-center">
- <i>Fig 1: MAM7 cloning and expression vector system</i>[1]
+ <figcaption class="figure-caption text-center"> <i>Fig 1: MAM7 cloning and expression vector system</i>[1]
</figcaption>
</figure>
-<p>With our progress in the project, our Wet Lab geared up on a detailed itinerary of planned experiments. This
+<p class="reveal-on-scroll">With our progress in the project, our Wet Lab geared up on a detailed itinerary of planned
+ experiments. This
curated
handbook includes various experiments like plasmid isolation, competent cell preparation, Ni-NTA column
chromatography, etc. The primary objective is to help other iGEM teams carry out these recombinant experiments and
provide them with the opportunity to improve their current experimental designs.</p>
-<p>With our progress in the project, our Wet Lab geared up on a detailed itinerary of experiments ready to go ahead
+<p class="reveal-on-scroll">With our progress in the project, our Wet Lab geared up on a detailed itinerary of
+ experiments ready to go ahead
with. Unfortunately due to lack of time we could not execute the lab work as planned. The <a
href="{{ url_for('pages', page='contribution') }}">contribution</a> page consists of our
experimentation handbook and a lab bulletin that summarises our journey so far in the lab. We curated a handbook
@@ -33,65 +35,55 @@
purification. Despite our iGEM season coming to an end soon enough, we intend to try to continue our
experimentation.</p>
-<h1>Experimentation Timeline</h1>
+<h1 class="reveal-on-scroll">Experimentation Timeline</h1>
-<p>Here's our week wise experimentation timeline!</p>
+<p class="reveal-on-scroll">Here's our week wise experimentation timeline!</p>
<div class="boxesParent">
- <div class="boxesGrid">
- <button type="button" class="btn btn-primary" data-bs-toggle="modal" data-bs-target="#wet-lab-modal-0">
- Week 0
- </button>
- <button type="button" class="btn btn-primary" data-bs-toggle="modal" data-bs-target="#wet-lab-modal-1">
- Week 1
- </button>
- <button type="button" class="btn btn-primary" data-bs-toggle="modal" data-bs-target="#wet-lab-modal-2">
- Week 2
- </button>
- <button type="button" class="btn btn-primary" data-bs-toggle="modal" data-bs-target="#wet-lab-modal-3">
- Week 3
- </button>
- <button type="button" class="btn btn-primary" data-bs-toggle="modal" data-bs-target="#wet-lab-modal-4">
- Week 4
- </button>
- <button type="button" class="btn btn-primary" data-bs-toggle="modal" data-bs-target="#wet-lab-modal-5">
- Week 5
- </button>
- <button type="button" class="btn btn-primary" data-bs-toggle="modal" data-bs-target="#wet-lab-modal-6">
- Week 6
- </button>
- <button type="button" class="btn btn-primary" data-bs-toggle="modal" data-bs-target="#wet-lab-modal-7">
- Week 7
- </button>
- </div>
+ <div class="boxesGrid"> <button type="button" class="btn btn-primary reveal-on-scroll" data-bs-toggle="modal"
+ data-bs-target="#wet-lab-modal-0"> Week 0
+ </button> <button type="button" class="btn btn-primary reveal-on-scroll" data-bs-toggle="modal"
+ data-bs-target="#wet-lab-modal-1"> Week 1
+ </button> <button type="button" class="btn btn-primary reveal-on-scroll" data-bs-toggle="modal"
+ data-bs-target="#wet-lab-modal-2"> Week 2
+ </button> <button type="button" class="btn btn-primary reveal-on-scroll" data-bs-toggle="modal"
+ data-bs-target="#wet-lab-modal-3"> Week 3
+ </button> <button type="button" class="btn btn-primary reveal-on-scroll" data-bs-toggle="modal"
+ data-bs-target="#wet-lab-modal-4"> Week 4
+ </button> <button type="button" class="btn btn-primary reveal-on-scroll" data-bs-toggle="modal"
+ data-bs-target="#wet-lab-modal-5"> Week 5
+ </button> <button type="button" class="btn btn-primary reveal-on-scroll" data-bs-toggle="modal"
+ data-bs-target="#wet-lab-modal-6"> Week 6
+ </button> <button type="button" class="btn btn-primary reveal-on-scroll" data-bs-toggle="modal"
+ data-bs-target="#wet-lab-modal-7"> Week 7
+ </button> </div>
</div>
-<div class="row">
- <!-- Week-0 -->
- <div class="row">
- <!-- Modal -->
+<div class="row"> <!-- Week-0 -->
+ <div class="row"> <!-- Modal -->
<div class="modal fade" id="wet-lab-modal-0" tabindex="-1" aria-labelledby="wet-lab-modal-0Label" aria-hidden="true"
style="width: 100%;">
<div class="modal-dialog modal-fullscreen wet-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
- <h3 class="modal-title" id="wet-lab-modal-0Label">Week 0</h3>
- <button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
+ <h3 class="modal-title reveal-on-scroll" id="wet-lab-modal-0Label">Week 0</h3> <button type="button"
+ class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-6">
<div class="row">
- <p>We visited the Enteric Disease Division at KMC, Manipal and observed the protocols for culturing,
- storage and detection of <i>Vibrio parahaemolyticus</i> strains under supervision.</p>
+ <p class="reveal-on-scroll">We visited the Enteric Disease Division at KMC, Manipal and observed the
+ protocols for culturing, storage and detection of <i>Vibrio parahaemolyticus</i> strains under
+ supervision.</p>
</div>
</div>
<div class="col-6">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-0-picture.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-0-picture.jpg"
class="figure-img img-fluid rounded" alt="Vibrio parahaemolyticus blood agar plate culture">
- <figcaption class="figure-caption text-center">
- <i>Vibrio parahaemolyticus</i> blood agar plate culture
+ <figcaption class="figure-caption text-center"> <i>Vibrio parahaemolyticus</i> blood agar plate
+ culture
</figcaption>
</figure>
</div>
@@ -101,94 +93,61 @@
</div>
</div>
</div>
-
<!-- Week-1 -->
- <div class="row">
- <!-- Modal -->
+ <div class="row"> <!-- Modal -->
<div class="modal fade" id="wet-lab-modal-1" tabindex="-1" aria-labelledby="wet-lab-modal-1Label" aria-hidden="true"
style="width: 100%;">
<div class="modal-dialog modal-fullscreen wet-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
- <h3 class="modal-title" id="wet-lab-modal-1Label">Week 1</h3>
- <button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
+ <h3 class="modal-title reveal-on-scroll" id="wet-lab-modal-1Label">Week 1</h3> <button type="button"
+ class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-6">
<div class="row" style="margin-left: 15px;">
- <ul>
+ <ul class="reveal-on-scroll">
<li>Primary Sub-culturing of <i>E. coli</i> DH5α.</li>
</li>
<li>Quantifying growth of Primary culture of DH5α</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b><br>
- OD<sub>600</sub> = 2.5
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b><br> OD<sub>600</sub> = 2.5
</p>
-
- <ul>
+ <ul class="reveal-on-scroll">
<li>Gram staining was performed for the first subculture.</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- <i>E. coli</i> DH5α cells were observed to be pink.
- <br>
- Cell debris were observed.
- </p>
-
- <p>
- <b class="wet-lab-troubleshoot">Troubleshoot</b>
- <br>
- Initial smearing could be done more gently.
- </p>
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> <i>E. coli</i> DH5α cells were
+ observed to be pink. <br> Cell debris were observed. </p>
+ <p class="reveal-on-scroll"> <b class="wet-lab-troubleshoot">Troubleshoot</b> <br> Initial smearing
+ could be done more gently. </p>
+ <ul class="reveal-on-scroll">
<li>Secondary subculture was performed</li>
<li>Quantifying growth of secondary subculture of the culture</li>
</ul>
-
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- OD<sub>600</sub> = 1.4
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> OD<sub>600</sub> = 1.4
</p>
-
- <ul>
+ <ul class="reveal-on-scroll">
<li>Gram staining was performed for the secondary subculture</li>
</ul>
-
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- <i>E. coli</i> DH5α cells were observed to be pink.
- <br>
- Some crystal violet stain residues were observed
- <br>
- Many cells were clumped together
- <br>
- Lesser cell debris were observed
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> <i>E. coli</i> DH5α cells were
+ observed to be pink. <br> Some crystal violet stain residues were observed
+ <br> Many cells were clumped together
+ <br> Lesser cell debris were observed
</p>
-
- <p>
- <b class="wet-lab-troubleshoot">Troubleshoot</b>
- <br>
- Washing with water can be done better and longer.
- </p>
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-troubleshoot">Troubleshoot</b> <br> Washing with water
+ can be done better and longer. </p>
+ <ul class="reveal-on-scroll">
<li>Multiple subcultures were prepared, and <i>E. Coli</i> DH5α growth was confirmed using
Indole test and Gram staining</li>
</ul>
</div>
</div>
<div class="col-6">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-1-gram-staining.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-1-gram-staining.jpg"
class="figure-img img-fluid rounded" alt="E.coli DH5-alpha gram staining observation">
- <figcaption class="figure-caption text-center">
- <i>E. coli</i> DH5α gram staining observation
+ <figcaption class="figure-caption text-center"> <i>E. coli</i> DH5α gram staining observation
</figcaption>
</figure>
</div>
@@ -198,76 +157,55 @@
</div>
</div>
</div>
-
<!-- Week-2 -->
- <div class="row">
- <!-- Modal -->
+ <div class="row"> <!-- Modal -->
<div class="modal fade" id="wet-lab-modal-2" tabindex="-1" aria-labelledby="wet-lab-modal-2Label" aria-hidden="true"
style="width: 100%;">
<div class="modal-dialog modal-fullscreen wet-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
- <h3 class="modal-title" id="wet-lab-modal-2Label">Week 2</h3>
- <button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
+ <h3 class="modal-title reveal-on-scroll" id="wet-lab-modal-2Label">Week 2</h3> <button type="button"
+ class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-6">
<div class="row" style="margin-left: 15px;">
- <ul>
+ <ul class="reveal-on-scroll">
<li>Primary culturing of pET-22b(+) transformed <i>E. coli</i> DH5α from prepared glycerol stock.
</li>
<li>Quantification of transformed <i>E. coli</i> culture. </li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b><br>
- OD<sub>600</sub> = 3
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b><br> OD<sub>600</sub> = 3
</p>
-
- <ul>
+ <ul class="reveal-on-scroll">
<li>Secondary culturing of <i>E. coli</i> DH5α. </li>
<li>Quantification of transformed <i>E. coli</i></li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- OD<sub>600</sub> = 0.7
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> OD<sub>600</sub> = 0.7
</p>
-
- <ul>
+ <ul class="reveal-on-scroll">
<li>Plasmid isolation of pET-22b(+) from transformed <i>E. coli</i> (Trial 1)</li>
<li>Quantification of Plasmid DNA. </li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- DNA concentration was found to be 38.1 ng/μL.
- </p>
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> DNA concentration was found to
+ be 38.1 ng/μL. </p>
+ <ul class="reveal-on-scroll">
<li>Gel electrophoresis to confirm the presence of isolated plasmid.</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br> - The plasmid was not observed.
- <br> - No RNA contamination was observed.
- </p>
-
- <p>
- <b class="wet-lab-troubleshoot">Troubleshoot</b>
- <br> - The culture was advised to be kept until an OD<sub>600</sub>value of 1-1.2 was obtained.
- <br> - The concentration of plasmid should be around 100 ng/μL.
- </p>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> - The plasmid was not observed.
+ <br> - No RNA contamination was observed. </p>
+ <p class="reveal-on-scroll"> <b class="wet-lab-troubleshoot">Troubleshoot</b> <br> - The culture was
+ advised to be kept until an OD<sub>600</sub>value of 1-1.2 was obtained. <br> - The concentration of
+ plasmid should be around 100 ng/μL. </p>
</div>
</div>
<div class="col-6">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-2-isolated-plasmid.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-2-isolated-plasmid.jpg"
class="figure-img img-fluid rounded" alt="pET-22b+ plasmid Isolation">
- <figcaption class="figure-caption text-center">
- pET-22b(+) plasmid Isolation:
- <br>
- No vector bands were observed
+ <figcaption class="figure-caption text-center"> pET-22b(+) plasmid Isolation: <br> No vector bands
+ were observed
</figcaption>
</figure>
</div>
@@ -277,64 +215,49 @@
</div>
</div>
</div>
-
<!-- Week-3 -->
- <div class="row">
- <!-- Modal -->
+ <div class="row"> <!-- Modal -->
<div class="modal fade" id="wet-lab-modal-3" tabindex="-1" aria-labelledby="wet-lab-modal-3Label" aria-hidden="true"
style="width: 100%;">
<div class="modal-dialog modal-fullscreen wet-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
- <h3 class="modal-title" id="wet-lab-modal-3Label">Week 3</h3>
- <button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
+ <h3 class="modal-title reveal-on-scroll" id="wet-lab-modal-3Label">Week 3</h3> <button type="button"
+ class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-6">
<div class="row" style="margin-left: 15px;">
- <ul>
+ <ul class="reveal-on-scroll">
<li>Primary subculturing of pET-22b(+) transformed <i>E. coli</i> DH5α from prepared transformed
- plates.
- </li>
+ plates. </li>
<li>Quantification of transformed <i>E. coli</i> culture </li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b><br>
- DH5α
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b><br> DH5α
<br> -> Culture 1 OD<sub>600</sub> = 0.4
<br> -> Culture 2 OD<sub>600</sub> = 0.6
</p>
-
- <ul>
+ <ul class="reveal-on-scroll">
<li>Secondary overnight sub-culturing of pET-22b(+) transformed <i>E. coli</i></li>
<li>Plasmid isolation of pET-22b(+) from transformed <i>E. coli</i> (Trial 2)</li>
<li>Quantification of Isolated plasmid. </li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- DNA concentration was found to be 69 ng/μL.
- </p>
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> DNA concentration was found to
+ be 69 ng/μL. </p>
+ <ul class="reveal-on-scroll">
<li>Gel electrophoresis for Isolated Plasmid</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- The plasmid was successfully isolated.
- </p>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> The plasmid was successfully
+ isolated. </p>
</div>
</div>
<div class="col-6">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-3-isolated-plasmid.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-3-isolated-plasmid.jpg"
class="figure-img img-fluid rounded" alt="pET-22b+ plasmid isolation.">
- <figcaption class="figure-caption text-center">
- pET-22b(+) plasmid isolation.
- <br>
- Vector bands were observed in well number 4 and 5
+ <figcaption class="figure-caption text-center"> pET-22b(+) plasmid isolation. <br> Vector bands were
+ observed in well number 4 and 5
</figcaption>
</figure>
</div>
@@ -344,68 +267,48 @@
</div>
</div>
</div>
-
<!-- Week-4 -->
- <div class="row">
- <!-- Modal -->
+ <div class="row"> <!-- Modal -->
<div class="modal fade" id="wet-lab-modal-4" tabindex="-1" aria-labelledby="wet-lab-modal-4Label" aria-hidden="true"
style="width: 100%;">
<div class="modal-dialog modal-fullscreen wet-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
- <h3 class="modal-title" id="wet-lab-modal-4Label">Week 4</h3>
- <button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
+ <h3 class="modal-title reveal-on-scroll" id="wet-lab-modal-4Label">Week 4</h3> <button type="button"
+ class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-6">
<div class="row" style="margin-left: 15px;">
- <ul>
+ <ul class="reveal-on-scroll">
<li>Primary Subculturing of pET-22b(+) transformed <i>E. coli</i> DH5α </li>
<li>Quantification of Transformed <i>E. coli</i></li>
</ul>
-
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- Glycerol stock culture OD<sub>600</sub> = 2.1
- <br>
- Plate culture OD<sub>600</sub>not measurable
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> Glycerol stock culture
+ OD<sub>600</sub> = 2.1
+ <br> Plate culture OD<sub>600</sub>not measurable
</p>
-
-
- <ul>
+ <ul class="reveal-on-scroll">
<li>Plasmid isolation and checking sample concentrations</li>
<li>Checking plasmid DNA concentration using nanocuvette</li>
</ul>
-
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- The concentration of DNA isolated from plate culture was found to be 48.2 ng/μL.
- <br>
- The concentration of DNA isolated from stock culture was found to be 18.3 ng/μL.
- </p>
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> The concentration of DNA
+ isolated from plate culture was found to be 48.2 ng/μL. <br> The concentration of DNA isolated from
+ stock culture was found to be 18.3 ng/μL. </p>
+ <ul class="reveal-on-scroll">
<li>Gel electrophoresis for isolated plasmid samples</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- The plasmid was successfully isolated.
- </p>
-
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> The plasmid was successfully
+ isolated. </p>
</div>
</div>
<div class="col-6">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-4-isolated-plasmid.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-4-isolated-plasmid.jpg"
class="figure-img img-fluid rounded" alt="pET-22b+ plasmid isolation.">
- <figcaption class="figure-caption text-center">
- pET-22b(+) plasmid isolation.
- <br>
- Vector bands were observed in well number 4 and 5
+ <figcaption class="figure-caption text-center"> pET-22b(+) plasmid isolation. <br> Vector bands were
+ observed in well number 4 and 5
</figcaption>
</figure>
</div>
@@ -415,139 +318,101 @@
</div>
</div>
</div>
-
<!-- Week-5 -->
- <div class="row">
- <!-- Modal -->
+ <div class="row"> <!-- Modal -->
<div class="modal fade" id="wet-lab-modal-5" tabindex="-1" aria-labelledby="wet-lab-modal-5Label" aria-hidden="true"
style="width: 100%;">
<div class="modal-dialog modal-fullscreen wet-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
- <h3 class="modal-title" id="wet-lab-modal-5Label">Week 5</h3>
- <button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
+ <h3 class="modal-title reveal-on-scroll" id="wet-lab-modal-5Label">Week 5</h3> <button type="button"
+ class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-6">
<div class="row" style="margin-left: 15px;">
- <ul>
+ <ul class="reveal-on-scroll">
<li>Single digestion of pET-22b(+). Was performed to check the efficiency of our enzymes. Reaction
1
was performed using NdeI enzyme. Reaction 2 was performed using the XhoI enzyme. </li>
<li>Gel electrophoresis of single Digested pET-22b(+)</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b><br>
- The digested band was in the 5.5kb range according to the DNA ladder, while the plasmid itself is
- 5493bp. Thus, single digestion of pET-22b(+) using NdeI and XhoI was successful.
- </p>
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b><br> The digested band was in the
+ 5.5kb range according to the DNA ladder, while the plasmid itself is
+ 5493bp. Thus, single digestion of pET-22b(+) using NdeI and XhoI was successful. </p>
+ <ul class="reveal-on-scroll">
<li>Double digestion of pET-22b(+) </li>
<li>Gel electrophoresis of double digested pET-22b(+) samples</li>
<li>Primary Subculturing of pET-22b(+) transformed <i>E. coli</i> DH5α </li>
<li>Quantification of Transformed <i>E. coli</i></li>
</ul>
-
- <p>
- <b class="wet-lab-result">Result</b>
- <br> - Plate culture 1: OD<sub>600</sub> = 0.2
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> - Plate culture 1:
+ OD<sub>600</sub> = 0.2
<br> - Plate Culture 2: OD<sub>600</sub> = 0.6
</p>
-
- <ul>
+ <ul class="reveal-on-scroll">
<li>Plasmid Isolation of pET-22b(+) from <i>E. coli</i> DH5α </li>
<li>Concentrations of Isolated plasmid </li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- The concentration of DNA from plate culture 1 was found to be 54.4 ng/μL.
- <br>
- The concentration of DNA from plate culture 2 was found to be 48.8 ng/μL.
- </p>
-
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> The concentration of DNA from
+ plate culture 1 was found to be 54.4 ng/μL. <br> The concentration of DNA from plate culture 2 was
+ found to be 48.8 ng/μL. </p>
+ <ul class="reveal-on-scroll">
<li>Gel electrophoresis of isolated plasmid samples </li>
<li>Dissolving lyophilized IDT MAM7 gene fragment </li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- The concentration of isolated plasmid was found to be 11.3 ng/μL.
- </p>
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> The concentration of isolated
+ plasmid was found to be 11.3 ng/μL. </p>
+ <ul class="reveal-on-scroll">
<li>PCR Amplification of MAM7</li>
<li>Running of gel electrophoresis of amplified MAM7</li>
<li>Purification of MAM7 amplicon using PCR cleanup kit</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- After purification, the concentration of purified MAM7 was way too low.
- </p>
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> After purification, the
+ concentration of purified MAM7 was way too low. </p>
+ <ul class="reveal-on-scroll">
<li>pET-22b(+) Plasmid Isolation using Miniprep Kit</li>
</ul>
-
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- Plasmid DNA concentration from plate culture 1 was found to be 55.1 ng/μL.
- <br>
- Plasmid DNA concentration from plate culture 2 was found to be 56.9 ng/μL.
- </p>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> Plasmid DNA concentration from
+ plate culture 1 was found to be 55.1 ng/μL. <br> Plasmid DNA concentration from plate culture 2 was
+ found to be 56.9 ng/μL. </p>
</div>
</div>
<div class="col-6">
<div class="row">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-5-double-digest.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-5-double-digest.jpg"
class="figure-img img-fluid rounded" alt="Double Digestion of pET22-B+.">
- <figcaption class="figure-caption text-center">
- Double Digestion of pET-22b(+)
- <br>
- The digested vector was observed In well number 6
+ <figcaption class="figure-caption text-center"> Double Digestion of pET-22b(+) <br> The digested
+ vector was observed In well number 6
</figcaption>
</figure>
</div>
-
<div class="row">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-5-isolated-plasmid.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-5-isolated-plasmid.jpg"
class="figure-img img-fluid rounded" alt="pET22-b+ plasmid isolation">
- <figcaption class="figure-caption text-center">
- pET-22b(+) plasmid isolation
- <br>
- Vector bands were observed in well number 4 and 5
+ <figcaption class="figure-caption text-center"> pET-22b(+) plasmid isolation
+ <br> Vector bands were observed in well number 4 and 5
</figcaption>
</figure>
</div>
-
<div class="row">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-5-mam7-pcr.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-5-mam7-pcr.jpg"
class="figure-img img-fluid rounded" alt="PCR Amplification of MAM7">
- <figcaption class="figure-caption text-center">
- PCR Amplification of MAM7
- <br>
- The amplified MAM7 gene was observed in well 2
+ <figcaption class="figure-caption text-center"> PCR Amplification of MAM7
+ <br> The amplified MAM7 gene was observed in well 2
</figcaption>
</figure>
</div>
-
<div class="row">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-5-single-digest.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-5-single-digest.jpg"
class="figure-img img-fluid rounded" alt="Single digestion of pET22-B+.">
- <figcaption class="figure-caption text-center">
- Single digestion of pET-22b(+)
- <br>
- The digested vector bands were observed in well number 4 and 5
+ <figcaption class="figure-caption text-center"> Single digestion of pET-22b(+) <br> The digested
+ vector bands were observed in well number 4 and 5
</figcaption>
</figure>
</div>
@@ -558,32 +423,27 @@
</div>
</div>
</div>
-
<!-- Week-6 -->
- <div class="row">
- <!-- Modal -->
+ <div class="row"> <!-- Modal -->
<div class="modal fade" id="wet-lab-modal-6" tabindex="-1" aria-labelledby="wet-lab-modal-6Label" aria-hidden="true"
style="width: 100%;">
<div class="modal-dialog modal-fullscreen wet-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
- <h3 class="modal-title" id="wet-lab-modal-6Label">Week 6</h3>
- <button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
+ <h3 class="modal-title reveal-on-scroll" id="wet-lab-modal-6Label">Week 6</h3> <button type="button"
+ class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-6">
<div class="row" style="margin-left: 15px;">
- <ul>
+ <ul class="reveal-on-scroll">
<li>PCR Amplification of the MAM7 gene</li>
<li>Dissolving lyophilized IDT MAM7 gene fragment</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b><br>
- OD<sub>600</sub> = 1.3 ng/μL.
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b><br> OD<sub>600</sub> = 1.3 ng/μL.
</p>
-
- <ul>
+ <ul class="reveal-on-scroll">
<li>MAM7 Primer Synthesis for AMplification of gene fragment</li>
<li>PCR Reaction Mix Prep for MAM7 gene amplification </li>
<li>PCR Gel Purification of Amplified MAM7</li>
@@ -591,49 +451,35 @@
<li>Chemical Preparation for PEG Method</li>
<li>Competent cell preparation using the PEG method</li>
</ul>
-
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- The molar concentration of glucose and Mg<sup>2+</sup> was rectified for the TSS preparation
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> The molar concentration of
+ glucose and Mg<sup>2+</sup> was rectified for the TSS preparation
needed
for
- competent cell preparation and transformation.
- </p>
-
- <ul>
+ competent cell preparation and transformation. </p>
+ <ul class="reveal-on-scroll">
<li>Transformation of pET-22b(+) was performed via PEG Method (Trial 1)</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- We determined the transformation efficiency and we obtained the highest number of isolated
- colonies.
- Hence, it was successful.
- </p>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> We determined the
+ transformation efficiency and we obtained the highest number of isolated
+ colonies. Hence, it was successful. </p>
</div>
</div>
<div class="col-6">
<div class="row">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-6-mam7-pcr.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-6-mam7-pcr.jpg"
class="figure-img img-fluid rounded" alt="PCR Amplification of MAM7.">
- <figcaption class="figure-caption text-center">
- PCR Amplification of MAM7.
- <br>
- The amplified MAM7 was observed in well 2, 3 and 4
+ <figcaption class="figure-caption text-center"> PCR Amplification of MAM7. <br> The amplified MAM7
+ was observed in well 2, 3 and 4
</figcaption>
</figure>
</div>
-
<div class="row">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-6-first-ligation.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-6-first-ligation.jpg"
class="figure-img img-fluid rounded" alt="1st Ligation of MAM7 insert and pET22-b+ vector">
- <figcaption class="figure-caption text-center">
- 1st Ligation of MAM7 insert and pET22-b+ vector
- <br>
- The ligated vectors were observed in well 4, 5 and 6
+ <figcaption class="figure-caption text-center"> 1st Ligation of MAM7 insert and pET22-b+ vector
+ <br> The ligated vectors were observed in well 4, 5 and 6
</figcaption>
</figure>
</div>
@@ -644,120 +490,88 @@
</div>
</div>
</div>
-
<!-- Week-7 -->
- <div class="row">
- <!-- Modal -->
+ <div class="row"> <!-- Modal -->
<div class="modal fade" id="wet-lab-modal-7" tabindex="-1" aria-labelledby="wet-lab-modal-7Label" aria-hidden="true"
style="width: 100%;">
<div class="modal-dialog modal-fullscreen wet-lab-modal-dialog modal-dialog-centered modal-dialog-scrollable">
<div class="modal-content">
<div class="modal-header">
- <h3 class="modal-title" id="wet-lab-modal-7Label">Week 7</h3>
- <button type="button" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
+ <h3 class="modal-title reveal-on-scroll" id="wet-lab-modal-7Label">Week 7</h3> <button type="button"
+ class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
</div>
<div class="modal-body">
<div class="row">
<div class="col-6">
<div class="row" style="margin-left: 15px;">
- <ul>
+ <ul class="reveal-on-scroll">
<li>Visualizing MAM7 ligated pET-22b(+)</li>
<li>Transformation of MAM7 ligated pET-22b(+) to <i>E. coli</i> DH5α by PEG method (Trial 2)</li>
</ul>
- <p>
- <b class="wet-lab-troubleshoot">Troubleshoot</b><br>
- Since the competent cells we prepared were a day old despite being stored at -80°C. Hence we
+ <p class="reveal-on-scroll"> <b class="wet-lab-troubleshoot">Troubleshoot</b><br> Since the competent
+ cells we prepared were a day old despite being stored at -80°C. Hence we
assumed
this to be the reason for our failed transformation
</p>
-
- <ul>
+ <ul class="reveal-on-scroll">
<li>Competent Cell preparation using the CaCl<sub>2</sub> method (Trial 1) </li>
<li>Transformation of MAM7 ligated pET-22b(+) to <i>E. coli</i> DH5α by CaCl<sub>2</sub> method
(Trial 1) </li>
<li>Competent cell preparation (Trial 2)</li>
<li>Colony PCR (Trial 1 of CaCl<sub>2</sub> method) = 5 mixtures from 1:3</li>
</ul>
-
- <p>
- <b class="wet-lab-result">Result</b>
- <br> -The vector to insert ratios yielded proper
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> -The vector to insert ratios
+ yielded proper
<br> - Colonies 1,2,4,6 and 10 showed the best
<br> - The MAM7 amplicon band (At around 2.5kb) was distinct for these colonies and showed the
least number of non-specific bands.
</p>
-
- <ul>
+ <ul class="reveal-on-scroll">
<li>Running of gel for Colony PCR (Trial 1)</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- No insert was observed. Instead, a big smear was observed at that location.
- </p>
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> No insert was observed.
+ Instead, a big smear was observed at that location. </p>
+ <ul class="reveal-on-scroll">
<li>Transformed pET-22b(+) plasmid Isolation using Miniprep Kit</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b><br>
- During the gel running, two bands were observed corresponding to one circular and one linear
- plasmid.
- But on checking for the concentration, the value was too low.
- </p>
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b><br> During the gel running, two
+ bands were observed corresponding to one circular and one linear
+ plasmid. But on checking for the concentration, the value was too low. </p>
+ <ul class="reveal-on-scroll">
<li>Colony PCR (Trial 2 of CaCl<sub>2</sub> method) = 3 mixtures from 1:2, 4 reaction mixtures
from
1:3
- transformed plates.
- <br>
- -> 3 reaction mixtures from 1:2 transformed plates
- <br>
- -> 4 reaction mixtures from 1:3 transformed plates
+ transformed plates. <br> -> 3 reaction mixtures from 1:2 transformed plates
+ <br> -> 4 reaction mixtures from 1:3 transformed plates
</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- After running the gel, we observed 3 bands, out of which we observed one band exactly below the
- insert. The reason for the other two nonspecific bands observed is yet to be troubleshot.
- </p>
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> After running the gel, we
+ observed 3 bands, out of which we observed one band exactly below the
+ insert. The reason for the other two nonspecific bands observed is yet to be troubleshot. </p>
+ <ul class="reveal-on-scroll">
<li>Transformed pET-22b(+) Isolation using Miniprep Kit</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br>
- We obtained a good concentration of the plasmid. After running the gel, we could observe only one
- single linear band.
- </p>
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> We obtained a good
+ concentration of the plasmid. After running the gel, we could observe only one
+ single linear band. </p>
+ <ul class="reveal-on-scroll">
<li>Single digestion of the transformed pET-22b(+) ligated with MAM7 using NdeI was carried out.
</li>
<li>We ran the gel and confirmed that our enzymes were working. </li>
<li>Double Digested transformed pET-22b(+) ligated with MAM7 using NdeI and XhoI Enzymes</li>
<li>Gel extraction was performed</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br> - 3/4th of the mixture was subjected to gel electrophoresis.
- <br> - After gel extraction, we obtained the concentration of pET-22b(+) and MAM7 after double
- digestion i.e. 5.5ng/μL and 5.6ng/μL, respectively.
- </p>
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> - 3/4th of the mixture was
+ subjected to gel electrophoresis. <br> - After gel extraction, we obtained the concentration of
+ pET-22b(+) and MAM7 after double
+ digestion i.e. 5.5ng/μL and 5.6ng/μL, respectively. </p>
+ <ul class="reveal-on-scroll">
<li>PCR Purification</li>
</ul>
- <p>
- <b class="wet-lab-result">Result</b>
- <br> - We obtained higher concentrations via PCR purification than Gel extraction.
- <br> - pET-22b(+) =17.1ng/μL, 17.7ng/μL=MAM7; were the obtained concentrations.
- </p>
-
- <ul>
+ <p class="reveal-on-scroll"> <b class="wet-lab-result">Result</b> <br> - We obtained higher
+ concentrations via PCR purification than Gel extraction. <br> - pET-22b(+) =17.1ng/μL,
+ 17.7ng/μL=MAM7; were the obtained concentrations. </p>
+ <ul class="reveal-on-scroll">
<li>pET-22b(+) and MAM7 Ligation
<br> - Higher ratios for vectors to insert were considered
<br> - Vector to Insert ratios that were ligated = 1:4(Gel Extracted), 1:5, 1:6(PCR Purified)
@@ -770,88 +584,74 @@
</div>
<div class="col-6">
<div class="row">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-single-digest.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-single-digest.jpg"
class="figure-img img-fluid rounded" alt="Single Digestion of transformed pET22-b+">
- <figcaption class="figure-caption text-center">
- Single digestion of transformed pET-22b(+)
+ <figcaption class="figure-caption text-center"> Single digestion of transformed pET-22b(+)
</figcaption>
</figure>
</div>
-
<div class="row">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-double-digest.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-double-digest.jpg"
class="figure-img img-fluid rounded" alt="Double Digestion of transformed pET22-b+">
- <figcaption class="figure-caption text-center">
- Double digestion of transformed pET-22b(+)
+ <figcaption class="figure-caption text-center"> Double digestion of transformed pET-22b(+)
</figcaption>
</figure>
</div>
-
<div class="row">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-ligation-try-2.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-ligation-try-2.jpg"
class="figure-img img-fluid rounded" alt="2nd Ligation of MAM7 insert and pET22-b+ vector">
- <figcaption class="figure-caption text-center">
- 2nd Ligation of MAM7 insert and pET-22b(+) vector
- <br>
- The ligated vectors were observed in well 5
+ <figcaption class="figure-caption text-center"> 2nd Ligation of MAM7 insert and pET-22b(+) vector
+ <br> The ligated vectors were observed in well 5
</figcaption>
</figure>
</div>
-
<div class="row">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-isolated-plasmid.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-isolated-plasmid.jpg"
class="figure-img img-fluid rounded" alt="pET-22b+ plasmid isolation.">
- <figcaption class="figure-caption text-center">
- pET-22b(+) plasmid isolation.
- <br>
- Vector bands were observed in well number 2 and 3
+ <figcaption class="figure-caption text-center"> pET-22b(+) plasmid isolation. <br> Vector bands were
+ observed in well number 2 and 3
</figcaption>
</figure>
</div>
-
<div class="row">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-colony-pcr-try-2.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-colony-pcr-try-2.jpg"
class="figure-img img-fluid rounded" alt="Colony PCR Trial 1">
- <figcaption class="figure-caption text-center">
- Colony PCR Trial 1
+ <figcaption class="figure-caption text-center"> Colony PCR Trial 1
</figcaption>
</figure>
</div>
-
<div class="row">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-colony-pcr-try-1.jpg"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-colony-pcr-try-1.jpg"
class="figure-img img-fluid rounded" alt="Colony PCR Trial 2">
- <figcaption class="figure-caption text-center">
- Colony PCR Trial 2
+ <figcaption class="figure-caption text-center"> Colony PCR Trial 2
</figcaption>
</figure>
</div>
-
<div class="row">
<div class="col-6">
<div class="row">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-cacl2-trial-2.png"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-cacl2-trial-2.png"
class="figure-img img-fluid rounded" alt="Transformed plates using CaCl2- Trial 1">
- <figcaption class="figure-caption text-center">
- Transformed plates using CaCl<sub>2</sub>- Trial 1
+ <figcaption class="figure-caption text-center"> Transformed plates using CaCl<sub>2</sub>- Trial
+ 1
</figcaption>
</figure>
</div>
</div>
<div class="col-6">
<div class="row">
- <figure class="figure">
- <img src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-cacl2-trial-1.png"
+ <figure class="figure reveal-on-scroll"> <img
+ src="https://static.igem.wiki/teams/4200/wiki/wet-lab-timeline/week-7-cacl2-trial-1.png"
class="figure-img img-fluid rounded" alt="Transformed plates using CaCl2- Trial 2">
- <figcaption class="figure-caption text-center">
- Transformed plates using CaCl<sub>2</sub>- Trial 2
+ <figcaption class="figure-caption text-center"> Transformed plates using CaCl<sub>2</sub>- Trial
+ 2
</figcaption>
</figure>
</div>
@@ -866,8 +666,9 @@
</div>
</div>
<br>
-<h1>Biosafety</h1>
-<p>Given the conversations we had with the experts in domains like Biosafety and Biosecurity, we decided to not work
+<h1 class="reveal-on-scroll">Biosafety</h1>
+<p class="reveal-on-scroll">Given the conversations we had with the experts in domains like Biosafety and Biosecurity,
+ we decided to not work
with the risk-2 organism <i>Vibrio parahaemolyticus</i>. Instead, we decided on expressing our target protein– MAM7
in
our chassis– <i>E. coli</i> and performing further experiments with it. Here we are making use of the truncated
@@ -878,11 +679,12 @@
dissemination, while complying with the laws and regulations of recombinant research provided by the university.
</p>
-<p>Further, in order to gain some on-sight experience, we visited Dr. Mamatha Ballal’s lab i.e., the Enteric Diseases
+<p class="reveal-on-scroll">Further, in order to gain some on-sight experience, we visited Dr. Mamatha Ballal’s lab
+ i.e., the Enteric Diseases
Division. Here we were provided with the opportunity to witness the culturing and maintenance of <i>V.
parahaemolyticus</i> stocks in a supervised environment.</p>
<b>References</b>
-<p>[1] Benchling [Biology Software]. (2022). Retrieved from https://benchling.com.</p>
+<p class="reveal-on-scroll">[1] Benchling [Biology Software]. (2022). Retrieved from https://benchling.com.</p>
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