diff --git a/wiki/pages/engineering.html b/wiki/pages/engineering.html index 2adf614e9390507cba45b85e6957779ea65c47ef..7aa79115d506095536427b5db6e55c2ace3f3971 100644 --- a/wiki/pages/engineering.html +++ b/wiki/pages/engineering.html @@ -5,15 +5,46 @@ {% block page_content %} -<div class="row mt-4"> +<div class="row"> <div class="col"> - <div class="bd-callout bd-callout-info"> - <h4>Silver Medal Criterion #1</h4> - <p>Demonstrate engineering success in a part of your project by going through at least one iteration of the engineering design cycle. This achievement should be distinct from your Contribution for Bronze.<p> - <p>If you plan to show engineering success by creating a new Part that has been shown to work as expected, you must document your contribution on the Part's Main Page on the <a href="http://parts.igem.org/Main_Page">Registry</a> for your team to be eligible for this criteria.</p> - <hr> - <p>Please see the <a href="https://competition.igem.org/judging/medals">2022 Medals Page</a> for more information.</p> - </div> + <h2><b>Engineering</b></h2> + <hr> + <h3><b>Design</b></h3> + <hr> + <p>At the design stage, we first did the preliminary research for our project. Through this, necessary enzymes and substances were identified, and expression vectors and genes were selected.<br> + The four enzymes we selected are as follows. Nitrite Reductase, Hydrazine Synthase, and Hydrazine Oxideneductase will be used for Nitrite (NO2-) and Ammonium (NH4+) removal.<br> + And superoxide dismutase enzymes will be used to perform H2O2 production.<br> + And we used this to construct parts. First, BBa_K4371000 (Anammox cluster: HZS + HZO + NIR) was created by clustering three enzymes used in Nitrite (NO2-) and Ammonium (NH4+) removal.<br> + It was inserted into pEXP5-NT, a specific cell free expression (CFE) vector. +Similarly, BBa_K38007 (SOD1 protein), a parts of Superoxide dismutase, an enzyme that conducts H2O2 production, was inserted into the expression vector pEXP5-NT. +</p> + + <h3><b>Build</b></h3> + <hr> + <p>In order to obtain the sequence found in the previous design stage, we ordered the sequence through Twist Bioscience. However, there was a problem that the sequence of the alpha subunit of the hydrazine synthase was longer than 1.8 kb. So we decided to get the gene by sequencing in the plasmid method.<br> + And we construct cell free expression system's experiment methods.</p> + + <h3><b>Test</b></h3> + <hr> + <p>The T7 promoter shows the most active protein expression at 25°C.<br> + <img src="https://static.igem.wiki/teams/4371/wiki/ic07-cfspt7.png"> + Figure. pT7 maximum Temperature (http://parts.igem.org/Help:Cell-free_chassis/Modelling#References)<br> + When checked through modeling, it can be seen that it is generated to the maximum at 25°C. </p> + + <h3><b>Learn</b></h3> + <hr> + <p>In order to obtain more production, it is necessary to replace it with plasmid origin with a higher CN value or with a stronger RBS. Through this, more enzymes can be obtained and it can have advantages in producing filters.</p> + + </div> +</div> + +<div class="row-mt-4"> + <div class="col"> + <h3><b>Reference</b></h3> + <hr> + <ul><li>http://parts.igem.org/Help:Cell-free_chassis/Modelling#References</li></ul> + + </div> </div> diff --git a/wiki/pages/model.html b/wiki/pages/model.html index a270b5fa0dc3e46b74da2afb0c668207d191c18f..2edf23c59eaaf01ba06a79a9c655de24efefcb92 100644 --- a/wiki/pages/model.html +++ b/wiki/pages/model.html @@ -19,4 +19,14 @@ </div> </div> +<div class="row"> + <div class="col"> + <h2><b>References</b></h2> + <hr> + <ul><li></li> + <li></li> + <li></li></ul> + </div> +</div> + {% endblock %}